CN112603956A - Traditional Chinese medicine compound preparation for preventing acute altitude stress - Google Patents
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Abstract
The invention belongs to the field of medicines, and discloses a traditional Chinese medicine compound preparation capable of preventing acute altitude stress: comprises ethanol extracts of herba Gei, caulis Sargentodoxae, herba Pyrolae, rhizoma Curculiginis, and medicinal adjuvants. The auxiliary materials comprise two or more of glycerol, water, ethanol, polyethylene glycol 400, dihydroxyaluminium aminoacetate, citric acid, sodium carboxymethylcellulose and sodium polyacrylate, and the traditional Chinese medicine compound cataplasm is prepared according to a proper preparation process. The scheme has good hypoxia resistance and fatigue resistance, rapid effect, safety and no toxicity, is suitable for people who rapidly advance into the plateau, and has good prevention effect on acute altitude reaction; the formulation of the scheme is cataplasm which is convenient to carry, transport and use and easy to accept by consumers.
Description
Technical Field
The invention belongs to the field of medicines, and particularly relates to a traditional Chinese medicine compound preparation for preventing acute altitude stress.
Background
The acute altitude reaction is a clinical syndrome which is frequently seen when personnel in plain regions rapidly advance to the plateau, the hypoxia of the plateau can influence the physiological functions of systems such as nerve, circulation, respiration, digestion, endocrine, immunity and the like of the organism, and particularly, for officers and soldiers in the region of the rapidly advancing plateau, the acute altitude reaction is a main cause which influences the body health and the military operation capacity of the officers and soldiers and reduces the maneuverability and the fighting capacity of the officers and soldiers. In addition, the implementation of national strategy is developed in the western world, which is bound to be accompanied by the development of more people to the plateau. Therefore, the medicine can prevent the occurrence of acute altitude stress and has important military and economic significance.
The only drug approved by the FDA in the United states for preventing and treating acute altitude sickness is acetazolamide, and adverse reactions such as numbness of limbs, tingling sensation, nausea, anorexia, drowsiness, diarrhea and the like are common. The Chinese patent medicine, namely the new compound radix codonopsitis tablet, the compound salvia miltiorrhiza tablet, the rhodiola rosea capsule and the like, has the effect of preventing and treating acute altitude reaction, but has long treatment course and is inconvenient to apply by people who are in rapid progress to the altitude. In addition, high altitude hypoxia may cause acute gastric mucosal injury, which is not conducive to absorption and utilization of oral drugs. There is therefore a need to find more convenient and feasible prophylactic agents.
Disclosure of Invention
Based on the problems, the invention provides a traditional Chinese medicine compound preparation for preventing acute altitude stress, which aims to solve the problems of adverse reaction and unfavorable drug absorption of the existing drugs.
The technical solution of the invention is as follows: a Chinese medicinal compound cataplasma for preventing acute altitude sickness comprises ethanol extracts of herba Gei, caulis Sargentodoxae, herba Pyrolae, and rhizoma Curculiginis, and medicinal adjuvants.
Further, the auxiliary materials comprise two or more of glycerol, water, ethanol, polyethylene glycol 400, dihydroxyaluminum glycinate, citric acid, sodium carboxymethylcellulose and sodium polyacrylate.
Further, the weight parts of the traditional Chinese medicine compound ethanol extract are 125-500 parts, and the pharmaceutic adjuvant comprises the following components in parts by weight: 75-400 parts of glycerol, 30-160 parts of absolute ethyl alcohol, 15-80 parts of polyethylene glycol 400, 20-100 parts of sodium polyacrylate, 4-20 parts of sodium carboxymethylcellulose, 5-25 parts of azone, 2.6-14 parts of dihydroxyaluminum glycinate, 5.6-30 parts of citric acid and 300-1600 parts of distilled water; the ethanol extract of the traditional Chinese medicine compound is obtained by heating, refluxing and extracting the aleppo avens bunge, the sargentgloryvine stem, the pyrola and the curculigo orchioides with 70% ethanol according to the weight part ratio of 2:2:1: 1.
Further, the preparation method of the traditional Chinese medicine compound ethanol extract comprises the following steps: adding 70% ethanol into the aleppo avens, the sargentgloryvine stem, the pyrola and the curculigo orchioides in a weight ratio of 2:2:1:1, wherein the addition amount of the ethanol is 8 times of the weight of the medicinal materials; then heating and refluxing for extraction, and carrying out reduced pressure concentration and freeze drying on the extracting solution to obtain the product.
Further, the preparation method of the traditional Chinese medicine preparation comprises the following steps:
(1) pulverizing herba Gei, caulis Sargentodoxae, herba Pyrolae, and rhizoma Curculiginis into coarse powder at a weight ratio of 2:2:1:1, mixing, adding 70% ethanol 8 times of the medicinal materials, extracting under heating and refluxing, concentrating the extractive solution under reduced pressure, and freeze drying to obtain effective component dry powder; mixing an appropriate amount of adjuvants a with the above dry powder of effective components to obtain phase A, wherein the adjuvants a are one or more of aluminum glycollate, citric acid, and distilled water;
(2) taking a proper amount of auxiliary materials B, wherein the auxiliary materials B are one or more of glycerol, absolute ethyl alcohol, polyethylene glycol 400, sodium polyacrylate, sodium carboxymethylcellulose and azone, and uniformly mixing the auxiliary materials B to obtain a phase B. Slowly adding the phase A into the phase B, and slowly stirring uniformly along the same direction to obtain a drug-loaded cataplasm matrix; coating the medicine-carrying cataplasm matrix in the inner ring of the non-woven fabric blank navel patch with a medicine spoon, covering with an isolation film, and standing, curing and molding to obtain the traditional Chinese medicine compound cataplasm.
Compared with the prior art, the scheme has the following beneficial effects:
the scheme has good prevention effect on acute altitude reaction, obvious hypoxia resistance and fatigue resistance, quick response, safety and no toxicity, and is suitable for people who rapidly advance the altitude. The formulation of the scheme is cataplasm which is convenient to carry, transport and use and easy to accept by consumers.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not used as limitations of the present invention.
Example 1:
a Chinese medicinal compound preparation for preventing acute altitude sickness comprises ethanol extracts of herba Gei, caulis Sargentodoxae, herba Pyrolae, and rhizoma Curculiginis, and medicinal adjuvants. The ethanol extract is prepared by adding 70% ethanol into herba Gei, caulis Sargentodoxae, herba Pyrolae and rhizoma Curculiginis at a weight ratio of 2:2:1:1, extracting under heating and refluxing, concentrating the extractive solution under reduced pressure, and freeze drying to obtain effective components; the medicinal auxiliary materials comprise an auxiliary material a and an auxiliary material b, wherein the auxiliary material a is dihydroxyaluminium aminoacetate, citric acid and distilled water, and the auxiliary material b is glycerol, absolute ethyl alcohol, polyethylene glycol 400, sodium polyacrylate, sodium carboxymethylcellulose and azone.
The preparation method of the traditional Chinese medicine compound preparation comprises the following steps:
(1): pulverizing herba Polygoni Avicularis, caulis Sargentodoxae, herba Pyrolae, and rhizoma Curculiginis into coarse powder, mixing, adding 70% ethanol (the weight of ethanol is 8 times of the coarse powder), heating and reflux-extracting for two times (each time for 1 hr), mixing the extractive solutions for 2 times, concentrating at 70 deg.C under reduced pressure, and freeze-drying at-20 deg.C for 96 hr to obtain dry powder of effective components. Weighing 125 parts of active ingredient dry powder, and uniformly mixing with an auxiliary material a (in the embodiment, the auxiliary material a consists of 2.6 parts of dihydroxyaluminum glycinate, 5.6 parts of citric acid and 300 parts of distilled water) to form a phase A;
(2): taking an auxiliary material B (in the embodiment, the auxiliary material B consists of 75 parts of glycerol, 30 parts of absolute ethyl alcohol, 15 parts of polyethylene glycol 400, 20 parts of sodium polyacrylate, 4 parts of sodium carboxymethyl cellulose and 5 parts of azone), uniformly stirring to obtain a phase B, slowly adding the phase A into the phase B, and slowly and uniformly stirring along the same direction to obtain the drug-loaded cataplasm matrix.
Coating the medicine carrying cataplasm matrix with spoon in the inner ring (inner diameter of 5cm) of non-woven fabric blank navel patch to a thickness of 3mm, coating with isolation film, standing, aging and molding to obtain the final product.
Example 2:
this example differs from example 1 only in that: 1. 250 parts of dry powder of active ingredients; 2. the auxiliary material a consists of 5.3 parts of dihydroxyaluminium glycinate, 11.4 parts of citric acid and 600 parts of distilled water; 3. the auxiliary material b consists of 150 parts of glycerol, 50 parts of absolute ethyl alcohol, 40 parts of polyethylene glycol 400, 50 parts of sodium polyacrylate, 10 parts of sodium carboxymethylcellulose and 12 parts of azone.
Example 3
This example differs from example 1 only in that: 1. 500 parts of dry powder of active ingredients are adopted; 2. the auxiliary material a consists of 14 parts of dihydroxyaluminium glycinate, 30 parts of citric acid and 1600 parts of distilled water; 3. the auxiliary material b consists of 400 parts of glycerol, 160 parts of absolute ethyl alcohol, 80 parts of polyethylene glycol 400, 100 parts of sodium polyacrylate, 20 parts of sodium carboxymethylcellulose and 25 parts of azone.
The scheme is also proved by pharmacodynamic and toxicological tests and the like, and has remarkable effect, safety and reliability:
firstly, pharmacodynamic study:
the low-pressure chamber simulates a plateau acute hypoxia model animal experiment:
the SD rats with 80 healthy males and the weight of 220 +/-20 g are randomly divided into a normal group, a blank matrix group, a traditional Chinese medicine compound cataplasm group and a positive control group, and the number of each group is 20.
Treatment of groups of rats:
the rats of the normal group were normally housed without any treatment.
Rats in the blank matrix group and the traditional Chinese medicine compound cataplasm group are anesthetized by injecting 10% chloral hydrate into abdominal cavity (0.3ml/100g) 6 hours before being placed in a low-pressure chamber, then the hairs on the abdominal part of the rats are removed by depilatory, and blank matrix (the A phase of the cataplasm does not contain active ingredient dry powder and only contains auxiliary material a) and traditional Chinese medicine compound cataplasm (containing traditional Chinese medicine compound active ingredient dry powder) are respectively coated on the umbilical part and the peripheral position. Rats generally recover from waking within 4 hours, and tear off the navel patch by themselves. Then let the rat rest for 2h, freely drink and eat water, and put into the low-pressure chamber.
Rats in the positive control group were subjected to acetazolamide intragastric administration 5 days before entering the low-pressure chamber, the dose was 25mg/kg, and the administration was continued for 5 days.
Except the normal group of rats, all the rats in the other groups were placed in a low-pressure chamber (model: YCP, product of Changsha Huaxi electronic technology Co., Ltd.) simulating a plateau environment with an altitude of 5000m, a vacuum pump was started and the rats were raised at a constant speed of 10m/s until the altimeter was stabilized at an altitude of 5000m for a duration of 12 hours. Rats all breathed hard, panting-like, and even died in the low-pressure chamber. The results show that the traditional Chinese medicine compound cataplasm can obviously improve the survival rate of rats with low pressure and oxygen deficiency, is superior to the positive control medicine, and is shown in the following table 1: survival rates of rats in each group.
TABLE 1 survival rates of rats in each group
And (3) fatigue resistance experiment: after the surviving rats were released from the low-pressure chamber, they were immediately placed on a rat wheel-type treadmill (model: XR-YLS-15A, product of Shanghai Xin Soft information technology Co., Ltd.) and a rat bar-rotating fatigue machine (model: XR1514, product of Shanghai Xin Soft information technology Co., Ltd.) for testing.
Running test: the rats run in a runner type running machine (the speed is constant at 15m/min), when the rats stop, electric shock is given (the voltage is 100V) until the rats run out (the running exhaustion judgment standard is that the rats stop running, the running cannot be continued for 20 seconds by continuous stimulation of an electrode plate, and the running exhaustion is judged at the moment), and the running time of the rats is recorded.
Rotating rod fatigue test: the rat rotameter is 90mm in diameter, accelerated from 0rpm to 20rpm in 6s, observed and recorded the time the rat can crawl on the rotarod (the computer automatically records the time it takes on the rod after the rat falls off the rotarod), and the average value is calculated after each rat has crawled 3 times.
Statistical processing is carried out on the data of the running exhaustion time and the rolling stick crawling time of the rat by SPSS22.0 software, single-factor variance analysis is carried out on each group of data, the data is expressed by mean +/-standard deviation, and the difference is considered to have statistical significance when P is less than 0.05. The results show that the traditional Chinese medicine compound cataplasm can obviously prolong the running exhaustion time (see the following table 2: the running exhaustion time of each group of rats) and the rod-rotating crawling time (see the following table 3: the rod-rotating crawling time of each group of rats) of the low-pressure and anoxic model rats:
TABLE 2 run out time for each group of rats
Note: p < 0.001, the difference was statistically significant compared to the blank matrix group.
TABLE 3 swivel crawling time for each group of rats
Note: p < 0.001, the difference was statistically significant compared to the blank matrix group.
In combination with the above, the traditional Chinese medicine compound cataplasm has better functions of resisting anoxia and fatigue.
II, toxicology research:
acute toxicity test of rabbit skin: randomly dividing 16 rabbits (with weight of 2.0 + -0.5 kg) into 4 groups, which are respectively intact skin blank matrix group, intact skin Chinese medicinal compound cataplasma group, damaged skin blank matrix group, and damaged skin Chinese medicinal compound cataplasma group, wherein the number of each group is 4Only, and the male and female halves. Depilating at the back of rabbit with electric hair clipper before administration, and depilating area is about 150cm2. The blank matrix group of damaged skin and the traditional Chinese medicine compound cataplasm group of damaged skin are firstly sterilized by iodophor, and then a sterile blade is used for depilating and dividing a well on the back, and the degree is determined by blood seepage.
Applying blank matrix on hair removal area of rabbits in intact skin blank matrix group and damaged skin blank matrix group, applying Chinese medicinal compound cataplasma on hair removal area of rabbits in intact skin Chinese medicinal compound cataplasma group and damaged skin Chinese medicinal compound cataplasma group, applying the paste for 24 hr, taking off, and eluting with warm water. After administration, the animals were observed for 14 days continuously, twice a day, and for changes in skin and hair, diet, stool and urine, respiration, eyes and mucous membrane, mental state, activity of limbs and death, and the animals were weighed before administration and 7 days and 14 days after administration, respectively. When the rabbit dies, necropsy and visual observation should be carried out in time, and when pathological changes are visible to the naked eye, pathological histological examination should be carried out.
The results show that: after the traditional Chinese medicine compound cataplasm is applied, the food, the stool, the breath, the eyes, the mucous membranes, the mental state, the activity of the limbs, the weight and the gloss of the fur of the rabbits in the complete skin traditional Chinese medicine compound cataplasm group and the damaged skin traditional Chinese medicine compound cataplasm group are not abnormal, wherein the damaged skin of the rabbits in the damaged skin traditional Chinese medicine compound cataplasm group starts to scab at the 2 nd day and is basically healed at about 6d after administration, no acute toxic reaction occurs in the observation period of 14d, no rabbit dies, and the difference is avoided compared with the blank matrix group.
Third, safety test
Rabbit skin irritation test: 12 rabbits (with a body weight of 2.0 + -0.5 kg, male and female halves) were randomly divided into two groups, the 1 st group was an intact skin group, and the 2 nd group was a damaged skin group. Before administration, hair was removed from the left and right sides of the back by electric hair clippers, and the length and width of the hair removal area were both 3 cm. The damaged skin group was first sterilized with iodophor and then depilated on the back with a sterile blade to isolate the "well" pattern, with blood leakage as the measure. The administration method adopts left and right self-control, and two groups of rabbits are applied with Chinese medicinal compound cataplasma at right hair removal area and blank matrix at left hair removal area, and the ointment is taken off and eluted with warm water after being applied for 24 hr. The local presence or absence of erythema and edema was visually observed at 1h, 24h, 48h, and 72h after the removal of the ointment, respectively.
The results show that: after the rabbits of the intact skin group and the damaged skin group remove the traditional Chinese medicine compound cataplasm for 1 hour, 24 hours, 48 hours and 72 hours, no obvious erythema and edema appear, no difference with the contrast position of the rabbits are generated, and no irritation reaction exists, so that the traditional Chinese medicine compound cataplasm is safe to use.
The scheme is characterized by comprising the following steps:
the medicine composition of the scheme is determined based on cell pharmacology experiments based on literature data. The blue cloth is reported in documents to have obvious effects of resisting fatigue, low temperature, oxygen deficiency, high temperature and stress, can relieve the damage of cerebral ischemia, improve blood and oxygen supply of the brain, and can eliminate phlegm and relieve asthma. The sargentgloryvine stem has the function of relaxing blood vessels, can improve the electrocardiogram ST segment of the myocardial infarction rabbit, reduce the myocardial infarction range and improve the myocardial lactic acid metabolism disorder caused by the myocardial infarction. The sargentgloryvine stem injection can improve the normal-pressure hypoxia tolerance or decompression survival ability of mice and prolong the survival time. Rhizoma Curculiginis has effects of resisting anoxia, tranquilizing mind, relieving convulsion, and resisting male hormone. The herba Pyrolae extract has protective effect on cerebral ischemia and myocardial ischemia reperfusion injury. Meanwhile, the aleppo avens, sargentgloryvine stem, curculigo orchioides and pyrola have strong effects of resisting oxidative damage. Oxidative damage is one of the major mechanisms of body damage in acute altitude sickness. Therefore, theoretically, the combination of the four traditional Chinese medicines of the aleppo avens, the sargentgloryvine stem, the pyrola herb and the curculigo orchioides can prevent the occurrence of acute altitude stress. The invention is based on the hypothesis that the compatibility of Chinese medicines is carried out.
Acute altitude reaction is a series of symptoms caused by the injury of heart, brain and the like due to acute hypoxia, so a cell pharmacology experiment adopts an injury model caused by oxygen deprivation reperfusion of neurons and cardiac muscle cells. Observing the protective effect of the medicament on the primary cultured rat cortical neurons and myocardial cell oxygen deprivation reperfusion injury model, wherein the tested medicament comprises extracts of single or multiple combinations of aleppo avens, sargentgloryvine stem, pyrola and curculigo orchioides. Pre-acting the tested medicine on the cortical spirit of ratPassing through neuron and myocardial cell, acting for 6 hr, removing medicine and culture medium, replacing with PBS, placing neuron and myocardial cell in three-gas incubator (95% N)2，5％CO2Incubation at 37 ℃ for 3h, followed by reperfusion, i.e.restoration of normal culture conditions, for 2 h.
The experimental result shows that the four traditional Chinese medicinal materials of the euonymus japonicus, the sargentgloryvine stem, the pyrola and the curculigo orchioides comprise the following components in parts by weight: 2:1:1, the 70% ethanol extract has good protection effect on rat cortical neurons and myocardial cells damaged by oxygen deprivation reperfusion, can obviously increase the survival rate of cells, reduce the apoptosis rate and reduce the activity of caspase-3. Therefore, according to the scheme, the aleppo avens bunge, the sargentgloryvine stem, the pyrola and the curculigo orchioides are screened out according to the weight proportion, and 70% ethanol extract of the aleppo avens bunge is taken as a test medicine for preventing acute altitude reaction.
Instructions for the selection of the cataplasma formulation: the cataplasm is an external patch taking water-soluble high molecular polymer as a matrix framework material, and has large drug-loading rate; the moisture retention is strong, and the compatibility with the skin is good; strong penetration and good controlled release effect; no toxicity, no stimulation and no allergy; the viscosity is controllable and moderate, and the patch can be repeatedly torn off and stuck. In the plateau environment, hypoxia can cause acute gastric mucosal injury, and most of the traditional medicines for preventing acute altitude reaction are orally taken, so that the absorption of the medicines is not facilitated, and the curative effect is influenced. The cataplasm well solves the problem, is beneficial to medicine absorption, avoids the first pass effect of the liver and improves the bioavailability. The Chinese medicine compound cataplasm is applied to the umbilical region and the peripheral skin, has less subcutaneous tissues of the umbilical region, poor barrier function and easy transdermal absorption of the medicine, directly diffuses to the venous network or the subclinical artery branch to enter the systemic circulation, thereby having quick response.
The above is an embodiment of the present invention. The embodiments and specific parameters in the embodiments are only for the purpose of clearly illustrating the process of verifying the invention and are not intended to limit the scope of the invention, which is defined by the claims, and all the equivalent structural changes made by applying the content of the specification of the invention should be covered by the scope of the invention. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and the scope of the invention is to be considered as the scope of the invention.
Claims (5)
1. A Chinese medicinal compound preparation for preventing acute altitude sickness is characterized by comprising ethanol extracts of aleppo avens, sargentgloryvine stem, pyrola herb and common curculigo rhizome and pharmaceutic adjuvants.
2. The compound traditional Chinese medicine preparation for preventing acute altitude stress as claimed in claim 1, wherein the adjuvant comprises two or more of glycerol, water, ethanol, polyethylene glycol 400, dihydroxyaluminium aminoacetate, citric acid, sodium carboxymethylcellulose and sodium polyacrylate.
3. The compound traditional Chinese medicine preparation for preventing acute altitude stress as claimed in claim 1, wherein the weight portion of the ethanol extract of the compound traditional Chinese medicine is 125-500 parts, and the pharmaceutical excipients comprise the following components in parts by weight: 75-400 parts of glycerol, 30-160 parts of absolute ethyl alcohol, 15-80 parts of polyethylene glycol 400, 20-100 parts of sodium polyacrylate, 4-20 parts of sodium carboxymethylcellulose, 5-25 parts of azone, 2.6-14 parts of dihydroxyaluminum glycinate, 5.6-30 parts of citric acid and 300-1600 parts of distilled water; the ethanol extract of the traditional Chinese medicine compound is obtained by heating, refluxing and extracting traditional Chinese medicinal materials of aleppo avens, sargentgloryvine stem, pyrola and curculigo orchioides with 70% ethanol according to the weight part ratio of 2:2:1: 1.
4. The compound traditional Chinese medicine preparation for preventing acute altitude stress as claimed in claim 3, wherein the preparation method of the ethanol extract of compound traditional Chinese medicine comprises the following steps: adding 70% ethanol into herba Gei, caulis Sargentodoxae, herba Pyrolae and rhizoma Curculiginis at a weight ratio of 2:2:1:1, heating and reflux-extracting, concentrating under reduced pressure, and freeze drying to obtain the final product.
5. The compound Chinese medicine preparation for preventing acute altitude stress as claimed in any one of claims 1-4, wherein the preparation method of the compound Chinese medicine preparation comprises the following steps:
(1) pulverizing herba Gei, caulis Sargentodoxae, herba Pyrolae, and rhizoma Curculiginis into coarse powder at weight ratio of 2:2:1:1, mixing, adding 70% ethanol into the coarse powder, heating for extraction, concentrating under reduced pressure, and freeze drying to obtain effective component dry powder; mixing an appropriate amount of adjuvants a with the above dry powder of effective components to obtain phase A, wherein the adjuvants a are one or more of aluminum glycollate, citric acid, and distilled water;
(2) taking a proper amount of auxiliary materials B, wherein the auxiliary materials B are one or more of glycerol, absolute ethyl alcohol, polyethylene glycol 400, sodium polyacrylate, sodium carboxymethylcellulose and azone, and uniformly mixing the auxiliary materials B to obtain a phase B. Slowly adding the phase A into the phase B, and slowly stirring uniformly along the same direction to obtain a drug-loaded cataplasm matrix; coating the medicine-carrying cataplasm matrix in the inner ring of the non-woven fabric blank navel patch with a medicine spoon, covering with an isolation film, and standing, curing and molding to obtain the traditional Chinese medicine compound cataplasm.
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| CN104095935A (en) * | 2014-06-25 | 2014-10-15 | 北京神石医药科技开发有限公司 | Chinese herbal preparation for preventing and curing acute mountain sickness and preparation method for Chinese herbal preparation |
| CN104644733A (en) * | 2015-03-18 | 2015-05-27 | 杨俊松 | Composition with function of improving anoxia endurance and health food of composition |
| CN104706771A (en) * | 2015-02-27 | 2015-06-17 | 杨俊松 | Composition with anti-anoxia and acute high-altitude reaction prevention and treatment effects |
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- 2021-01-11 CN CN202110029233.4A patent/CN112603956A/en active Pending
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| CN104095935A (en) * | 2014-06-25 | 2014-10-15 | 北京神石医药科技开发有限公司 | Chinese herbal preparation for preventing and curing acute mountain sickness and preparation method for Chinese herbal preparation |
| CN104706771A (en) * | 2015-02-27 | 2015-06-17 | 杨俊松 | Composition with anti-anoxia and acute high-altitude reaction prevention and treatment effects |
| CN104644733A (en) * | 2015-03-18 | 2015-05-27 | 杨俊松 | Composition with function of improving anoxia endurance and health food of composition |
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