CN112586595A - Chinese herbal medicine micro-ecological compound preparation for preventing and treating newborn piglet enteritis and preparation method and application thereof - Google Patents
Chinese herbal medicine micro-ecological compound preparation for preventing and treating newborn piglet enteritis and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets, and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine for livestock and the technical field of feed additives. Is prepared by adding lactobacillus plantarum and starch-eating lactobacillus liquid into the extracting solution of Chinese herbal medicines and performing anaerobic fermentation; the weight ratio of the lactobacillus liquid to the Chinese herbal medicines is 1-3: 40-50. Wherein, the Chinese herbal medicines comprise the following components in parts by weight: 2-8 parts of astragalus membranaceus, 2-6 parts of turpinia formosana, 2-6 parts of dandelion, 2-6 parts of folium isatidis and 1-5 parts of folium artemisiae argyi. The Chinese herbal medicine micro-ecological compound preparation provided by the invention can be used for pig feed production and prevention and treatment of newborn piglet enteritis. By utilizing the synergistic effect of the compatibility of the lactobacillus and the Chinese herbal medicines, the functions of improving the immunity of the organism, inhibiting harmful bacteria, improving the microbial environment of the intestinal tract and the like are achieved. Can effectively prevent and treat piglet bacterial enteritis.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines for livestock and the technical field of feed additives, in particular to a Chinese herbal medicine microecological compound preparation for preventing and treating enteritis of newborn piglets and a preparation method and application thereof.
Background
In recent years, with the advance of large-scale live pig breeding, the problems existing in the breeding are more and more prominent, the disease control of the live pigs is better performed, the survival rate of piglets is effectively improved, and the breeding income is further improved, so that the breeding method becomes one of the key problems needing to be researched in live pig breeding at the present stage. The diarrhea is one of common diseases of piglets, has a wide epidemic range, can reduce the daily gain and the feed conversion rate of the piglets, causes the growth of the piglets to be slow, causes the death rate of the piglets to be increased, and is a main factor for damaging the growth performance of the piglets. The previous control measures for piglet diarrhea are mainly to use antibiotics, and long-term use of the antibiotics can cause pathogenic bacteria to generate drug resistance, so that the autoimmunity of piglets is reduced. Therefore, the search for a safer and more effective piglet diarrhea prevention and treatment strategy becomes a problem which needs to be solved urgently at present. 194 bulletin issued by the rural part of national agriculture, and since 1 month and 1 day of 2020, the medicinal feed additive will be completely withdrawn in 2020. The Chinese herbal medicine microecological preparation has the advantages of no toxic or side effect, no drug residue, low production cost, remarkable effect and the like, and is widely concerned. The Chinese herbal medicine microecological preparation has a certain resistance effect on pathogenic microorganisms, can influence the proliferation of acid-sensitive microorganisms by reducing the pH value in intestinal tracts, can stimulate the intestinal mucosa immune response, and improves the intestinal tract microbial environment, thereby having the effects of preventing and treating piglet diarrhea and improving the survival rate and the resistance of piglets. Therefore, the research and development of novel, safe and efficient antibiotic substitutes are necessary conditions for guaranteeing the healthy and sustainable development of the animal husbandry.
Disclosure of Invention
Aiming at the technical problems, the invention aims to provide a Chinese herbal medicine microecological composite preparation for preventing and treating enteritis of newborn piglets, and a preparation method and application thereof.
In order to realize the aim, the Chinese herbal medicine micro-ecological compound preparation for preventing and treating the enteritis of newborn piglets is prepared by adding lactobacillus liquid into an extracting solution of Chinese herbal medicines and performing anaerobic fermentation; the weight ratio of the lactobacillus liquid to the Chinese herbal medicines is 1-3: 40-50.
Preferably, the lactobacillus in the lactobacillus liquid comprises a mixture of lactobacillus plantarum and lactobacillus amylovorus.
Preferably, the Chinese herbal medicines comprise the following components in parts by weight: 2-8 parts of astragalus membranaceus, 2-6 parts of turpinia formosana, 2-6 parts of dandelion, 2-6 parts of folium isatidis and 1-5 parts of folium artemisiae argyi.
The invention also provides a preparation method of the Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets, which comprises the following specific steps:
(1) combining radix astragali, folium Turpiniae, herba Taraxaci, folium Isatidis and folium Artemisiae Argyi by weight parts into compound Chinese medicine, adding 3 times of water by volume, boiling for 3 times, each time for 50min, mixing the 3 decoctions and concentrating to obtain Chinese herbal medicine concentrated solution;
(2) adding MRS broth into the concentrated Chinese medicinal liquid to obtain culture solution, adjusting pH to 7.0, sterilizing with 121 deg.C high pressure steam for 20min, cooling to room temperature, inoculating lactobacillus liquid into the culture solution according to weight ratio, fermenting at 37 deg.C for 72h, and packaging to obtain Chinese medicinal microecological compound preparation.
Preferably, the crude drug content in the Chinese herbal medicine concentrated solution in the step (1) is 0.5 g/mL.
Preferably, the Chinese herbal medicine micro-ecological compound preparation in the step (2) is stored at 4 ℃ for later use.
The invention also provides application of the Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets in pig feed.
The invention also provides application of the Chinese herbal medicine micro-ecological compound preparation for preventing and treating newborn piglet enteritis in preventing and treating newborn piglet enteritis.
The medicinal properties and pharmacology of the Chinese herbal medicine micro-ecological compound preparation are described as follows:
radix astragali has effects of invigorating qi, consolidating superficial resistance, expelling toxin, expelling pus, and promoting urination. Can be used for treating deficiency of vital energy, asthenia, chronic diarrhea, proctoptosis, spontaneous perspiration, edema, uterine prolapse, albuminuria due to chronic nephritis, diabetes, etc.
The folium Turpiniae has effects of clearing away heat and toxic materials, relieving sore throat, relieving swelling, resisting bacteria, relieving pain and regulating immunity. Can be used for treating swelling and pain of throat, pyocutaneous disease, toxic swelling, traumatic injury, etc.
The dandelion has the effects of clearing away heat and toxic materials, promoting urination, removing stasis, resisting tumors, resisting gastric ulcer, benefiting gallbladder, protecting liver and the like, and has good killing effect on pathogenic microorganisms such as dysentery bacillus, typhoid bacillus and the like and helicobacter pylori and the like. Can be used for treating lymphadenitis, gastritis, furunculosis, acute conjunctivitis, common cold, fever, acute tonsillitis, and acute bronchitis.
Folium Artemisiae Argyi has effects of warming channels, stopping bleeding, eliminating dampness, dispelling cold, relieving asthma, relieving cough, eliminating phlegm, etc., and can be used for treating lower abdomen psychroalgia, deficiency cold hemorrhage, fetal leakage, menoxenia, cold womb infertility, etc.
Folium Isatidis has effects of resisting pathogenic microorganism, resisting endotoxin, resisting inflammation, enhancing immunity, and relieving fever, and can be used for treating fever, hyperpyrexia, polydipsia, dysentery, unconsciousness, jaundice, erysipelas, pharyngitis, aphtha, etc.
The lactobacillus has the functions of improving the intestinal microbial environment, regulating immunity, resisting inflammation and the like, and can inhibit the growth and the propagation of pathogenic bacteria by competing limited nutrient substances and interacting with the pathogenic bacteria. In animal production, the Chinese herbal medicines and probiotics such as lactobacillus have synergistic effect, and have the effects of improving immunity of organisms, inhibiting harmful bacteria and the like.
The invention has the beneficial effects that:
1) safe and reliable, green and environment-friendly, and conforms to the modern healthy culture concept.
2) By utilizing the synergistic effect of the compatibility of the probiotics and the Chinese herbal medicines, the intestinal microecological system of the newborn piglets is helped to be established in advance, the immunity of the organism is improved, and the colonization of harmful bacteria is inhibited by improving the microbial environment of the intestinal tract.
3) By adopting the theory of maternal-infant microecology, the probiotic lactobacillus comes from the birth canal of a healthy sow and the intestinal tract of a healthy piglet, can effectively prevent and treat piglet bacterial enteritis and improve the daily gain and health condition of the piglet.
4) The Chinese herbal medicine compound preparation containing the turpinia formosana leaves and the probiotic lactobacillus are compatible and applied to preventing the diarrhea of the piglets for the first time.
Detailed Description
In order to more clearly and completely describe the technical scheme of the invention, the invention is further described in detail by the specific embodiments, and it should be understood that the specific embodiments described herein are only used for explaining the invention, and are not used for limiting the invention, and various changes can be made within the scope defined by the claims of the invention.
Example 1 screening of Lactobacillus plantarum R23 and Lactobacillus amylovorus K2
1. Preliminary screening
And separating the dominant bacteria lactobacillus in the intestinal tracts of the healthy sows and the intestinal tracts of the healthy piglets based on the analysis result of the intestinal flora of the healthy/diarrhea piglets and the analysis result of the intestinal flora of the healthy/endometritis sows. Collecting 10 parts of excrement samples of healthy sows and healthy piglets respectively, diluting the samples to proper gradients in a gradient mode respectively by using normal saline, coating the samples on an MRS culture medium, and culturing to obtain 8 strains of lactic acid bacteria, wherein the temporary names are as follows: k1, K2, 12.1, 12.2, H1, R12, R22, R23.
The preparation method of the MRS liquid culture medium comprises the following steps: 10.0g of peptone, 5.0g of beef extract powder, 4.0g of yeast extract powder, 2.0g of triammonium citrate, 20.0g of glucose, 801.0 mL of tween, 5.0g of sodium acetate, 2.0g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, 0.05g of manganese sulfate, pH 6.0-6.4 and 1000mL of distilled water, and sterilizing by high-pressure steam for later use.
The preparation method of the MRS solid culture medium comprises the following steps: 10.0g of peptone, 5.0g of beef extract powder, 4.0g of yeast extract powder, 2.0g of triammonium citrate, 20.0g of glucose, 801.0 mL of tween, 5.0g of sodium acetate, 2.0g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, 0.05g of manganese sulfate, 2% of agar, 6.0-6.4 of pH and 1000mL of distilled water, and sterilizing by high-pressure steam for later use.
The preparation method of the LB liquid culture medium comprises the following steps: 10g of peptone, 10g of sodium chloride, 5g of yeast extract, pH 7.0 and 1000mL of distilled water, and sterilizing the mixture by high-pressure steam for later use.
The preparation method of the LB solid medium comprises the following steps: 10g of peptone, 10g of sodium chloride, 5g of yeast extract, 2% of agar, pH 7.0 and 1000mL of distilled water, and sterilizing the mixture by high-pressure steam for later use.
The TSC solid medium preparation method comprises the following steps: 15.0g of tryptone, 5.0g of soytone, 5.0g of yeast powder, 1.0g of sodium metabisulfite, 1.0g of ferric ammonium citrate, 2% of agar, 7.4-7.8 of pH, 1000mL of distilled water and high-pressure steam sterilization for later use.
The FTG liquid medium preparation method comprises the following steps: 15.0g of casein, 5.0g of yeast extract powder, 5.0g of glucose, 0.5g of sodium thioglycollate, 0.5g of L-cystine, 2.5g of sodium chloride, 0.001g of resazurin, 0.75g of agar, 6.9-7.3 of pH, 1000mL of distilled water and high-pressure steam sterilization for later use.
2. Double sieve
And (3) indication bacteria: common bacteria Escherichia coli K88 and salmonella causing piglet bacterial enteritis are inoculated in LB liquid culture solution and cultured for 12h at 37 ℃ and 180 rpm; and (3) inoculating clostridium perfringens into the FTG culture solution, and performing static culture for 12h at 42 ℃.
Preparing a lactic acid bacteria liquid: the lactic acid bacteria obtained by primary screening and separation are inoculated in MRS liquid culture solution and are statically cultured for 24 hours at 37 ℃.
In vitro bacteriostasis test: respectively and uniformly coating the escherichia coli K88 cultured for 12 hours, the salmonella and the clostridium perfringens suspension on an LB plate and a TSC plate by adopting an agar diffusion method, placing an oxford cup on the plates, respectively adding the lactobacillus suspension cultured for 24 hours into the oxford cup, standing at 37 ℃ for 12 hours, and observing the result.
Through in vitro antibacterial tests, lactobacillus which has obvious inhibition effect on the growth of Escherichia coli K88, clostridium perfringens and salmonella is obtained by screening from the birth canal of a healthy sow and the intestinal tract of a healthy piglet respectively and is named as R23 and K2 respectively. The antibacterial comprehensive effect is optimal, and the antibacterial abilities of the strain and other lactobacillus strains separated from a laboratory are compared as shown in the following table 1:
table 1: determination of bacteriostatic ability of lactobacillus strains
From table 1, it can be seen that the lactobacillus R23 and K2 screened out in the laboratory have more obvious bacteriostatic action than other lactobacilli, and can effectively inhibit the proliferation of common pathogenic bacteria of livestock and poultry.
The selected strains are subjected to 16Sr DNA molecular identification, and the results of NCBI sequence comparison show that the strains with the highest similarity (100%) to lactobacillus R23 are lactobacillus plantarum, and the strains with the highest similarity (100%) to lactobacillus K2 are lactobacillus amylovorus.
EXAMPLE 2 preparation of Chinese herbal medicine micro-ecological composite preparation
1) Mixing 40 parts of astragalus membranaceus, 30 parts of turpinia formosana, 30 parts of dandelion, 25 parts of folium isatidis, 18 parts of folium artemisiae argyi and five Chinese herbal medicines uniformly, adding 3 times of water with volume of 3 times, boiling for 50min each time, combining 3 times of decoction, and concentrating to obtain the compound Chinese herbal medicine with crude drug content of 0.5 g/mL.
2) Adding the compound Chinese herbal medicine prepared in the step 1) into MRS broth to prepare a culture solution, adjusting the pH value to 7.0, sterilizing the mixture for 30min at 115 ℃ by high-pressure steam, cooling the mixture to room temperature, inoculating lactobacillus plantarum R23 and lactobacillus amylovorus K2 into the culture solution in an inoculation amount of 3%, and fermenting the mixture for 72h at 37 ℃ to prepare the Chinese herbal medicine microecological compound preparation I.
Example 3
1) Mixing radix astragali 10 parts, folium Turpiniae 30 parts, herba Taraxaci 10 parts, folium Isatidis 30 parts, folium Artemisiae Argyi 5 parts, and five Chinese medicinal materials, adding 3 times of water, boiling for 3 times, each time for 50min, mixing the decoctions for 3 times, and concentrating to obtain compound Chinese medicinal concentrated solution with crude drug content of 0.5 g/mL.
2) Adding the compound Chinese herbal medicine prepared in the step 1) into MRS broth to prepare a culture solution, adjusting the pH value to 7.0, sterilizing the mixture for 30min at 115 ℃ by high-pressure steam, cooling the mixture to room temperature, inoculating the lactobacillus solution into the culture solution according to the weight ratio of 1:40, and fermenting the mixture for 72h at 37 ℃ to prepare the Chinese herbal medicine microecological composite preparation.
Example 4
1) Mixing 40 parts of astragalus membranaceus, 10 parts of turpinia formosana, 30 parts of dandelion, 10 parts of folium isatidis, 25 parts of folium artemisiae argyi and five Chinese herbal medicines uniformly, adding 3 times of water by volume and boiling for 3 times, 50min each time, combining 3 times of decoction, and concentrating to obtain a compound Chinese herbal medicine concentrated solution with the crude drug content of 0.5 g/mL.
2) Adding the compound Chinese herbal medicine prepared in the step 1) into MRS broth to prepare a culture solution, adjusting the pH value to 7.0, sterilizing the mixture for 30min at 115 ℃ by high-pressure steam, cooling the mixture to room temperature, inoculating lactobacillus liquid into the culture solution according to the weight ratio of 3:50, and fermenting the mixture for 72h at 37 ℃ to prepare the Chinese herbal medicine microecological composite preparation.
Comparative example 1 preparation of a Chinese herbal medicine micro-ecological composite preparation
Mixing radix astragali 40 parts, herba Taraxaci 30 parts, folium Isatidis 25 parts, folium Artemisiae Argyi 18 parts, and four Chinese medicinal materials, adding 3 times of water, boiling for 3 times, each time for 50min, mixing the decoctions for 3 times, and concentrating to obtain compound Chinese medicinal material with crude drug content of 0.5 g/mL.
Adding the compound Chinese herbal medicine prepared in the step 1) into MRS broth to prepare a culture solution, adjusting the pH value to 7.0, sterilizing the mixture for 30min at 115 ℃ by high-pressure steam, cooling the mixture to room temperature, inoculating lactobacillus plantarum R23 and lactobacillus amylovorus K2 into the culture solution in an inoculation amount of 3%, and fermenting the mixture for 72h at 37 ℃ to prepare a Chinese herbal medicine microecological compound preparation II.
Example 5 viable count of Chinese herbal medicine micro-ecological composite preparation
Taking 1mL of the Chinese herbal medicine micro-ecological compound preparation prepared in the example 2 and the comparative example 1, and carrying out 10 and 10 times of treatment by using sterile normal saline2、103、……、108Diluting by multiple times, transferring 200uL to 2 uL of each bacterial liquid by a sterile pipetteAnd (3) uniformly coating in an MRS agar plate by using a sterile triangular coating rod, placing in an anaerobic environment at 37 ℃ for inverted culture for 24h after the bacteria liquid is completely absorbed, and counting in groups. Measuring viable lactobacillus concentration in the Chinese herbal medicine microecological composite preparation to be 6 × 107cfu/ml。
Example 6 determination of bacteriostatic ability of Chinese herbal medicine micro-ecological composite preparation in vitro
Uniformly coating Escherichia coli K88, salmonella and Clostridium perfringens suspension cultured for 12h on an LB plate and a TSC plate respectively by adopting an agar diffusion method, placing an Oxford cup on the plates, adding the Chinese herbal medicine microecological composite preparation I (group I) in the example 2, the Chinese herbal medicine microecological composite preparation II (group II) in the comparative example 1, the compound Chinese herbal medicine decoction (group III) and the lactic acid bacteria suspension (group IV) cultured for 72h into the Oxford cup respectively, standing at 37 ℃ for 12h, and observing the result.
In vitro antibacterial tests show that the Chinese herbal medicine microecological compound preparation has obvious inhibition effect on the growth of Escherichia coli K88, Clostridium perfringens and Salmonella, and the antibacterial abilities of the groups are compared as shown in the following table 2:
table 2: determination of bacteriostatic ability of Chinese herbal medicine microecological compound preparation
From table 2, it can be seen that the bacteriostatic effect of the Chinese herbal medicine microecological compound preparation i containing the turpinia arguta leaf component in example 2 is better than that of the Chinese herbal medicine microecological compound preparation ii without the turpinia arguta leaf component in comparative example 1, and the bacteriostatic ability of the Chinese herbal medicine microecological compound preparation is stronger than that of the compound Chinese herbal medicine decoction and the lactic acid bacteria solution.
Application example 1: influence of the product of the invention on the growth performance of piglets
Test protocol: 96 newborn piglets with similar body weight and good health condition are randomly divided into 4 groups, each group has 4 repetitions, and each repetition has 6 repetitions. I is a control group, and basic ration is fed; II is 0.1 percent of Chinese herbal medicine microecological compound preparation group, and 0.1 percent of Chinese herbal medicine microecological compound preparation I is added into the basic daily ration; III is 0.2 percent of Chinese herbal medicine microecological compound preparation group, and 0.2 percent of Chinese herbal medicine microecological compound preparation I is added into basic daily ration; IV is 0.3% Chinese herbal medicine microecological compound preparation group, and 0.3% Chinese herbal medicine microecological compound preparation I is added into the basic daily ration. The test period was 30 days, and the piglets and the feed were weighed on the first and last days of the test to calculate average daily feed intake, average daily gain and feed-to-meat ratio of the piglets. And observing the shape of the excrement of the piglets and whether the anus of the piglets has excrement adhesion or other dysentery symptoms three times a day in the morning, at noon and at night, and calculating the diarrhea rate of the piglets in the test period.
Data processing: the experimental data were analyzed for variance using ANOVA in SPSS 21.0 software, and multiple comparisons using LSD, with the results expressed as "mean. + -. standard deviation" and significance level P < 0.05.
Test results and analysis: the influence of the herbal microecological compound preparation on the production performance and diarrhea rate of piglets is provided. See table 3 below.
TABLE 3 influence of the Chinese herbal medicine micro-ecological compound preparation on the productivity and diarrhea rate of piglets
Note: the same letter is used for indicating that the difference is not significant (P >0.05) and the different lower case letters indicate that the difference is significant (P < 0.05).
As can be seen from Table 3, compared with the control group, the average daily feed intake and daily weight gain of piglets in each treatment group are both significantly improved (P is less than 0.05), wherein the average daily feed intake and daily weight gain of group III (added with 0.2% of the Chinese herbal medicine microecological compound preparation) are the highest; the feed conversion ratio and diarrhea rate are obviously reduced (P <0.05), wherein the feed conversion ratio and diarrhea rate of the group III are the lowest.
Application example 2: influence of the product of the invention on the growth performance of piglets
Test protocol: 96 newborn piglets with similar body weight and good health condition are randomly divided into 4 groups, each group has 4 repetitions, and each repetition has 6 repetitions. I is a control group, and basic ration is fed; II is a 0.2% herbal microecological complex group, to which 0.2% herbal microecological complex I was added to the basal diet (example 2); III is a Chinese herbal medicine micro-ecological compound preparation group without turpinia formosana components with 0.2 percent, and 0.2 percent of Chinese herbal medicine micro-ecological compound preparation II is added into basic daily ration (comparative example 1); IV is 0.2% of compound Chinese herbal medicine, and 0.2% of compound Chinese herbal medicine is added into basic daily ration. The test period was 30 days, and the piglets and the feed were weighed on the first and last days of the test to calculate average daily feed intake, average daily gain and feed-to-meat ratio of the piglets. And observing the shape of the excrement of the piglets and whether the anus of the piglets has excrement adhesion or other dysentery symptoms three times a day in the morning, at noon and at night, and calculating the diarrhea rate of the piglets in the test period.
Data processing: the experimental data were analyzed for variance using ANOVA in SPSS 21.0 software, and multiple comparisons using LSD, with the results expressed as "mean. + -. standard deviation" and significance level P < 0.05.
Test results and analysis: the influence of the herbal microecological compound preparation on the production performance and diarrhea rate of piglets is provided. See table 3 below.
TABLE 4 influence of the Chinese herbal medicine micro-ecological compound preparation on the productivity and diarrhea rate of piglets
Note: the same letter is used for indicating that the difference is not significant (P >0.05) and the different lower case letters indicate that the difference is significant (P < 0.05).
As can be seen from Table 4, compared with the control group, the average daily feed intake and daily weight gain of piglets in each treatment group are both significantly improved (P is less than 0.05), wherein the average daily feed intake and daily weight gain of group II (added with the Chinese herbal medicine microecological compound preparation I containing the turpinia formosana leaf component) are the highest; the diarrhea rate is obviously reduced (P <0.05), wherein the diarrhea rate of the group II is the lowest; compared with a control group and a group III (added with a Chinese herbal medicine microecological compound preparation II without the turpinia formosana leaf component), the feed-meat ratio of the piglets of the group II is obviously reduced (P is less than 0.05).
From the above experimental examples, it can be seen that the 0.2% Chinese herbal medicine microecological compound preparation I in example 2 has the best addition effect, which is superior to the single addition effect of the compound Chinese herbal medicine, and shows that the lactobacillus and the Chinese herbal medicine have significant synergistic effect; the adding effect of the Chinese herbal medicine microecological composite preparation I containing the turpinia arguta leaf component in the example 2 is better than that of the Chinese herbal medicine microecological composite preparation II without the turpinia arguta leaf component in the comparative example 1, and the turpinia arguta leaf component in the Chinese herbal medicine microecological composite preparation I has a remarkable effect of promoting the growth of piglets. It should be noted that the Chinese herbal medicine micro-ecological compound preparations prepared in examples 3-4 have the same effect as the preparation prepared in example 2.
In conclusion, the Chinese herbal medicine microecological compound preparation is applied to piglet production, can obviously improve the average daily feed intake and average daily gain of piglets, reduce the feed conversion ratio, enhance the immunity, maintain the intestinal homeostasis, obviously reduce the diarrhea rate and the probability of other diseases, improve the survival rate of piglets, and has no pollution to the environment on the premise of improving the economic benefit of livestock breeding.
Finally, it should be emphasized that the above-described preferred embodiments of the present invention are merely examples of implementations, rather than limitations, and that many variations and modifications of the invention are possible to those skilled in the art, without departing from the spirit and scope of the invention.
Claims (8)
1. A Chinese herbal medicine micro-ecological compound preparation for preventing and treating newborn piglet enteritis is prepared by adding lactobacillus liquid into Chinese herbal medicine extract, and performing anaerobic fermentation; the weight ratio of the lactobacillus liquid to the Chinese herbal medicines is 1-3: 40-50.
2. The Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets according to claim 1, which is characterized in that: the lactobacillus in the lactobacillus liquid comprises the mixture of lactobacillus plantarum and lactobacillus amylovorus.
3. The Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets according to claim 1, which is characterized in that: the Chinese herbal medicines comprise the following components in parts by weight: 2-8 parts of astragalus membranaceus, 2-6 parts of turpinia formosana, 2-6 parts of dandelion, 2-6 parts of folium isatidis and 1-5 parts of folium artemisiae argyi.
4. The preparation method of the Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets according to any one of claims 1 to 3, is characterized by comprising the following specific steps:
(1) combining radix astragali, folium Turpiniae, herba Taraxaci, folium Isatidis and folium Artemisiae Argyi by weight parts into compound Chinese medicine, adding 3 times of water by volume, boiling for 3 times, each time for 50min, mixing the 3 decoctions and concentrating to obtain Chinese herbal medicine concentrated solution;
(2) adding MRS broth into the concentrated Chinese medicinal liquid to obtain culture solution, adjusting pH to 7.0, sterilizing with 115 deg.C high pressure steam for 30min, cooling to room temperature, inoculating lactobacillus liquid into the culture solution according to weight ratio, fermenting at 37 deg.C for 72 hr, and packaging to obtain Chinese medicinal microecological compound preparation.
5. The preparation method of the Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets according to claim 4, wherein the crude drug content in the Chinese herbal medicine concentrated solution in the step (1) is 0.5 g/mL.
6. The preparation method of the Chinese herbal medicine microecological compound preparation for preventing and treating enteritis of newborn piglets according to claim 4, wherein the Chinese herbal medicine microecological compound preparation in the step (2) is preserved for standby at 4 ℃.
7. The use of the Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets, which is disclosed by any one of claims 1 to 3, in pig feed.
8. The use of the Chinese herbal medicine micro-ecological compound preparation for preventing and treating enteritis of newborn piglets, which is disclosed by any one of claims 1 to 3, in preventing and treating enteritis of newborn piglets.
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