CN112574883B - Device for culturing animal muscle cells - Google Patents

Device for culturing animal muscle cells Download PDF

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Publication number
CN112574883B
CN112574883B CN202011480209.4A CN202011480209A CN112574883B CN 112574883 B CN112574883 B CN 112574883B CN 202011480209 A CN202011480209 A CN 202011480209A CN 112574883 B CN112574883 B CN 112574883B
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barrel
liquid
culture dish
dish body
container
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CN112574883A (en
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王可
谭秀文
刘昭华
王建英
崔绪奎
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Institute Animal Science and Veterinary Medicine of Shandong AAS
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Institute Animal Science and Veterinary Medicine of Shandong AAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/14—Pressurized fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Microbiology (AREA)
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  • Biomedical Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Clinical Laboratory Science (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides a device for culturing animal muscle cells, and belongs to the technical field of cell culture. The technical scheme is as follows: the device for culturing the animal muscle cells is characterized by comprising a culture dish body and a dish cover, wherein a sealed cavity is arranged at the lower part of the culture dish body, a liquid inlet pipe communicated with the cavity is arranged in the culture dish body, and a liquid outlet of the liquid inlet pipe is higher than the height of the liquid level in the culture dish body and the height of the liquid level in the cavity; the culture dish is characterized by further comprising a container which is communicated with the cavity through a one-way valve and can be adjusted in a liquid storage space, the liquid storage space of the container is reduced, and liquid in the container can pass through the sealed cavity and the liquid inlet pipe into the culture dish body. The invention has the beneficial effects that: the culture solution can be added into the culture dish under the condition that the culture dish is not moved and the cover is opened by people.

Description

Device for culturing animal muscle cells
Technical Field
The invention relates to the technical field of cell culture, in particular to a device for culturing animal muscle cells.
Background
Cell culture usually uses a culture flask or a culture dish. The culture bottle has a small diameter of the bottle mouth, so that the operation is not easy when tissue particles are inoculated in primary culture. The most commonly used today is the petri dish, which suffers from the following disadvantages: firstly, the cover is loosely covered on the culture dish, and the cover can be carelessly moved when the culture dish is taken, so that the culture solution grows; secondly, the side wall of the culture dish is not high, and the culture solution is shaken on the cover by slightly large action amplitude during taking, so that the culture solution can grow bacteria, which is not beneficial to the observation of a microscope.
In the primary culture, after tissue particles to be inoculated adhere to the wall, the culture dish needs to be taken out from the culture box and placed in a super clean bench, and the culture dish is placed back to the culture box after culture solution is added. In the whole moving process, operators can carefully and meticulously operate to avoid causing the growth of bacteria in the culture solution, and time and labor are wasted.
Disclosure of Invention
The invention aims to provide an apparatus for culturing animal muscle cells, which can assist the manual addition of a culture solution into a culture dish without moving the culture dish and opening a cover during primary culture.
The invention is realized by the following measures: the device for culturing the animal muscle cells is characterized by comprising a culture dish body and a dish cover, wherein a sealed cavity is arranged at the lower part of the culture dish body, a liquid inlet pipe communicated with the cavity is arranged in the culture dish body, and a liquid outlet of the liquid inlet pipe is higher than the height of the liquid level in the culture dish body and the height of the liquid level in the cavity;
still include through check valve one with the container that the stock solution space of cavity intercommunication can be adjusted, the stock solution space of container diminishes and to make liquid in the container passes through sealed cavity and the feed liquor pipe gets into this is internal in the culture dish.
The extrusion mechanism is used for controlling the liquid storage space in the container to be reduced;
the extrusion mechanism comprises an extrusion barrel and a first sealing piston arranged in the extrusion barrel, and the container is arranged in the extrusion barrel and below the first sealing piston.
Still including control actuating mechanism of sealed piston one, actuating mechanism include with the stock solution bucket that the extrusion bucket is connected, be provided with the fluid in the stock solution bucket, the stock solution bucket pass through fluid current-limiting structure with the extrusion bucket intercommunication, just be located on the extrusion bucket the top of sealed piston one is provided with toward extrude the outside carminative blast pipe of bucket, be provided with check valve two on the blast pipe, the fluid enters into gradually in the extrusion bucket the action of gravity of fluid is down sealed piston one moves down.
The liquid storage barrel comprises a conical connecting barrel at the lower part, a cylindrical barrel at the upper part and a connecting pipe which is connected with the conical connecting barrel and the cylindrical barrel and shrinks towards the axis, and the fluid flow limiting structure is arranged on the connecting pipe.
The fluid flow limiting structure is a flow limiting valve or a plurality of first plugs all provided with water outlet holes, and the inner diameters of the water outlet holes on the first plugs are different.
The first sealing piston is fixed on the upper part of the side wall of the extrusion barrel through an elastic piece.
The container is a water bag made of flexible materials.
The container and the extrusion barrel are integrally arranged, the bottom of the extrusion barrel is sealed through a bottom plate, and the container is arranged between the bottom plate of the extrusion barrel and the first sealing piston.
The fluid is oil or water.
The feed liquor pipe is followed the inner wall equipartition of culture dish body has a plurality of, the feed liquor pipe all with the cavity intercommunication.
The culture dish comprises a culture dish body and is characterized by further comprising a liquid drainage mechanism arranged below the culture dish body, wherein the liquid drainage mechanism comprises a liquid drainage barrel, the liquid drainage barrel is arranged below the culture dish body, a plurality of liquid drainage holes are formed in the culture dish body, an annular baffle is arranged below the culture dish body and on the periphery of the liquid drainage holes, a second plug for plugging the liquid drainage holes is arranged on the annular baffle, and the second plug is arranged below the culture dish body through a spring;
a sealing piston II is arranged in the liquid discharge barrel, a U-shaped pipe is externally connected to the upper portion of the side wall of the liquid discharge barrel, the upper outlet and the lower outlet of the U-shaped pipe are communicated with the inside of the liquid discharge barrel, a liquid discharge port is formed in the side wall of the liquid discharge barrel and located below the U-shaped pipe, and the thickness of the sealing piston II is smaller than the minimum distance between the two outlets.
The lower end of the liquid discharge barrel is sealed, a piston rod is concentrically arranged on the sealing piston II, and the piston rod is arranged on the liquid discharge barrel through threaded connection.
The culture dish rack comprises a base, and the culture dish body is placed on the base;
still including fixed the solid fixed ring of stock solution bucket, gu fixed ring cover is in the outside of toper connecting cylinder, gu fixed ring's the outside is provided with the connecting plate, the below of connecting plate is provided with the screw rod, the lower extreme of screw rod stretches out the base passes through the nut and fixes.
Compared with the prior art, the invention has the beneficial effects that: the culture solution can be added into the culture dish under the condition that the culture dish is not moved and the cover is opened by people.
Drawings
Fig. 1 is a schematic overall structure diagram of the first embodiment.
Fig. 2 is a schematic view of an internal structure of the first embodiment.
Fig. 3 is a schematic view of the internal structure of the squeezing barrel according to the first embodiment.
Fig. 4 is a schematic overall structure diagram of the second to sixth embodiments.
Fig. 5 is a schematic view illustrating an inner structure of an extruding bucket according to embodiments two to six.
Fig. 6 is a schematic internal structure diagram of the second to sixth embodiments.
Fig. 7 is a partial enlarged view of fig. 6 at B.
Fig. 8 is a schematic structural view of the drain tank.
Wherein the reference numbers are: 1. a culture dish body; 2. a dish cover; 3. extruding the barrel; 4. a first sealing piston; 5. a liquid storage barrel; 6. a container; 7. an exhaust pipe; 8. a liquid discharge mechanism; 10. a pipeline; 11. placing a rack; 12. a fixing ring; 101. a cavity; 102. a liquid inlet pipe; 103. a liquid discharge hole; 104. an annular baffle; 401. an elastic member; 402. a support bar; 501. a tapered connecting cylinder; 502. a cylindrical barrel; 503. a connecting pipe; 504. a first plug; 505. a water outlet hole; 801. a liquid discharge barrel; 802. a second sealing piston; 803. a piston rod; 804. a second plug; 805. a spring; 806. a U-shaped pipe; 807. a liquid discharge port; 1201. a connecting plate; 1202. a screw.
Detailed Description
In order to clearly illustrate the technical features of the present solution, the present solution is explained below by way of specific embodiments.
It should be noted that the embodiments and features of the embodiments of the present invention may be combined with each other without conflict.
The first embodiment is as follows:
referring to fig. 1-3, a device for culturing animal muscle cells comprises a culture dish body 1 and a dish cover 2, wherein a sealed cavity 101 is arranged at the lower part of the culture dish body 1, a liquid inlet pipe 102 communicated with the cavity 101 is arranged in the culture dish body 1, and a liquid outlet of the liquid inlet pipe is higher than the liquid level in the culture dish body 1 and the liquid level in the cavity 101;
the culture dish further comprises a container 6 which is communicated with the cavity 101 through a one-way valve and can adjust the liquid storage space, and the liquid storage space of the container 6 is reduced, so that liquid in the container 6 can enter the culture dish body 1 through the sealed cavity 101 and the liquid inlet pipe 102.
The extrusion mechanism is used for controlling the liquid storage space in the container 6 to be reduced;
the extrusion mechanism comprises an extrusion barrel 3 and a first sealing piston 4 arranged in the extrusion barrel 3, and a container 6 is arranged in the extrusion barrel 3 and below the first sealing piston 4.
Still including the actuating mechanism of control sealed piston 4, actuating mechanism includes the stock solution bucket 5 of being connected with extrusion bucket 3, be provided with the fluid in the stock solution bucket 5, stock solution bucket 5 passes through fluid current-limiting structure and extrudes bucket 3 intercommunication, the top that just is located sealed piston 4 on the extrusion bucket 3 is provided with toward 3 outside carminative blast pipes 7 of extrusion bucket, be provided with check valve two on the blast pipe 7, the fluid enters into extrusion bucket 3 gradually in, sealed piston 4 moves down under fluidic action of gravity.
The liquid storage barrel 5 comprises a conical connecting barrel 501 at the lower part, a cylindrical barrel 502 at the upper part and a connecting pipe 503 which is used for connecting the conical connecting barrel 501 and the cylindrical barrel 502 and is contracted towards the axis, and the fluid flow limiting structure is arranged on the connecting pipe 503.
The fluid flow limiting structure is a flow limiting valve or a plurality of plugs I504 each provided with a water outlet 505, and the inner diameters of the water outlets 505 on different plugs I504 are different.
The sealing piston one 4 is fixed to the upper portion of the sidewall of the pressing tub 3 by an elastic member 401. The elastic member 401 may be a spring or a rubber band.
The container 6 and the extrusion barrel 3 are integrally arranged, the bottom of the extrusion barrel 3 is sealed through a bottom plate, and the container is arranged between the bottom plate of the extrusion barrel 3 and the first sealing piston 4.
A plurality of liquid inlet pipes 102 are evenly distributed along the inner wall of the culture dish body 1, and the liquid inlet pipes 102 are communicated with the cavity 101.
The use mode is as follows: according to the volume of the culture solution injected into the culture dish body 1, a container 6 with a proper volume is selected, proper fluid is injected into the liquid storage barrel 5, the fluid can drip to the upper side of the first sealing piston 4 through the water outlet 505 on the first plug 504, along with the lapse of time, more and more fluid is accumulated on the first sealing piston 4, the air on the upper part of the extrusion barrel 3 can be extruded out through the exhaust valve in the entering process of the fluid, when the weight of the fluid can overcome the friction force of the first sealing piston 4 and the pulling force of the elastic part 401, the first sealing piston 4 can move downwards, the space of the container 6 is smaller and smaller in the moving downwards process, the nutrient solution in the container 6 can be extruded into the cavity 101, and along with the continuous falling of the first sealing piston 4, the culture solution can enter the culture dish body 1 through the cavity 101 and the liquid inlet pipe 102. The volume in the container 6 only needs to be ensured by adding the nutrient solution once, and in addition, the size of the water outlet hole 505 can control the time of adding the nutrient solution into the culture dish body 1, and the smaller the volume is, the longer the waiting time of adding the nutrient solution is.
Example two:
referring to fig. 4-8, a device for culturing animal muscle cells comprises a culture dish body 1 and a dish cover 2, wherein a sealed cavity 101 is arranged at the lower part of the culture dish body 1, a liquid inlet pipe 102 communicated with the cavity 101 is arranged in the culture dish body 1, and a liquid outlet of the liquid inlet pipe is higher than the liquid level in the culture dish body 1 and the liquid level in the cavity 101;
the culture dish further comprises a container 6 which is communicated with the cavity 101 through a one-way valve and can adjust the liquid storage space, and the liquid storage space of the container 6 is reduced, so that liquid in the container 6 can enter the culture dish body 1 through the sealed cavity 101 and the liquid inlet pipe 102.
The extrusion mechanism is used for controlling the liquid storage space in the container 6 to be reduced;
the extrusion mechanism comprises an extrusion barrel 3 and a first sealing piston 4 arranged in the extrusion barrel 3, and a container 6 is arranged in the extrusion barrel 3 and below the first sealing piston 4.
Still including the actuating mechanism of control sealed piston 4, actuating mechanism includes the stock solution bucket 5 of being connected with extrusion bucket 3, be provided with the fluid in the stock solution bucket 5, stock solution bucket 5 passes through fluid current-limiting structure and extrudes bucket 3 intercommunication, the top that just is located sealed piston 4 on the extrusion bucket 3 is provided with toward 3 outside carminative blast pipes 7 of extrusion bucket, be provided with check valve two on the blast pipe 7, the fluid enters into extrusion bucket 3 gradually in, sealed piston 4 moves down under fluidic action of gravity.
The liquid storage barrel 5 comprises a conical connecting barrel 501 at the lower part, a cylindrical barrel 502 at the upper part and a connecting pipe 503 which is used for connecting the conical connecting barrel 501 and the cylindrical barrel 502 and is contracted towards the axis, and the fluid flow limiting structure is arranged on the connecting pipe 503.
The fluid flow limiting structure is a flow limiting valve or a plurality of plugs I504 which are provided with water outlet holes 505, and the inner diameters of the water outlet holes 505 on the different plugs I504 are different.
The sealing piston one 4 is fixed to the upper portion of the sidewall of the pressing tub 3 by an elastic member 401. The elastic frame 401 is fixed by a support bar 402 fixed on the inner wall of the pressing tub 3, and the elastic member 401 may be a spring or a rubber band.
The container 6 and the extrusion barrel 3 are integrally arranged, the bottom of the extrusion barrel 3 is sealed through a bottom plate, and the container is arranged between the bottom plate of the extrusion barrel 3 and the first sealing piston 4.
The liquid inlet pipe 102 is evenly distributed with a plurality of liquid inlet pipes 102 along the inner wall of the culture dish body 1, and the liquid inlet pipes 102 are communicated with the cavity 101.
The culture dish further comprises a liquid discharging mechanism 8 arranged below the culture dish body 1, wherein the liquid discharging mechanism 8 comprises a liquid discharging barrel 801, the liquid discharging barrel 801 is arranged below the culture dish body 1, a plurality of liquid discharging holes 103 are formed in the culture dish body 1, an annular baffle plate 104 is arranged below the culture dish body 1 and positioned on the periphery of the liquid discharging holes 103, a second plug 804 for plugging the liquid discharging holes 103 is arranged on the annular baffle plate 104, and the second plug 804 is arranged below the culture dish body 1 through a spring 805;
a second sealing piston 802 is arranged in the liquid discharge barrel 801, a U-shaped pipe 806 is externally connected to the upper portion of the side wall of the liquid discharge barrel 801, the upper outlet and the lower outlet of the U-shaped pipe 806 are communicated with the inside of the liquid discharge barrel 801, a liquid discharge port 807 is arranged on the side wall of the liquid discharge barrel 801 and below the U-shaped pipe 806, and the thickness of the second sealing piston 802 is smaller than the minimum distance between the two outlets.
The lower end of the liquid discharge barrel 801 is sealed, a piston rod 803 is concentrically arranged on the sealing piston II 802, and the piston rod 803 is arranged on the liquid discharge barrel 801 through threaded connection.
The use method comprises the following steps: the liquid discharge mechanism 8 is adjusted in advance so that the sealing piston 802 is positioned between the two outlets, the liquid discharge barrel 801 is communicated with the outside air due to the existence of the U-shaped pipe 806 and the liquid discharge port 807, and at the moment, the second plug 804 completely seals the liquid discharge hole 103 under the action of the spring 805.
Then according to the amount of the culture solution injected into the culture dish body 1, a container 6 with a proper volume is selected, proper fluid is injected into the liquid storage barrel 5, the fluid can drip above the first sealing piston 4 through the water outlet 505 on the first plug 504, along with the lapse of time, more and more fluid is accumulated on the first sealing piston 4, the air on the upper part of the extrusion barrel 3 can be extruded out through the exhaust valve in the entering process of the fluid, when the weight of the fluid can overcome the friction force of the first sealing piston 4 and the tensile force of the elastic piece 401, the first sealing piston 4 can move downwards, the space of the container 6 is smaller and smaller in the moving downwards process, the nutrient solution in the container 6 can be extruded into the cavity 101, and along with the continuous falling of the first sealing piston 4, the culture solution can enter the interior of the culture dish body 1 through the cavity 101 and the liquid inlet pipe 102. The volume in the container 6 only needs to be ensured by one time of adding, and in addition, the size of the water outlet hole 505 can control the time of adding the nutrient solution into the culture dish body 1, and the smaller the size is, the longer the waiting time for adding the nutrient solution is.
When the culture solution needs to be replaced or the waste culture solution needs to be discharged, the piston rod 803 can be rotated to enable the second sealing piston 802 to move downwards, when the outlet below the U-shaped pipe 806 is blocked, the air is not introduced above the liquid discharging barrel 801, the piston rod 803 is continuously rotated, the second sealing piston 802 continuously moves downwards, the space above the liquid discharging barrel 801 is gradually increased, the quality of air is unchanged, the air pressure is reduced, the air pressure at the upper part of the culture dish body 1 is greater than that at the upper part of the liquid discharging barrel 801, when the air pressure at the upper part of the culture dish body 1 can overcome the pulling force of the spring 805, the second sealing plug 804 is pushed open, waste liquid flows to the upper part of the sealing piston 802 through the liquid discharging hole 103, and due to the fact that the inner diameter of the liquid discharging hole 103 is small, tissue particles cannot flow out, and can be attached to the inner wall of the culture dish body 1, and the difficulty of flow out is increased. The second sealing piston 802 descends continuously, when the liquid outlet 807 is higher than the second sealing piston 802, the waste liquid flows out through the liquid outlet 807, and the spring 805 drives the second plug 804 to reset.
Example three:
in the first or second embodiment, the conical connection cylinder 501 is detachably connected, preferably screwed, to the squeezing barrel 3, the container 6 is detachably connected to the culture dish body 1 through the existing pipe 10, and the first check valve is disposed on the pipe 10. Thus, the cleaning and the disinfection at the later stage can be convenient.
Example four:
on the basis of the first embodiment, the second embodiment or the third embodiment, the culture dish device further comprises a placing frame 11, wherein the placing frame 11 comprises a base, and the culture dish body 1 is placed on the base;
still including the solid fixed ring 12 of fixed liquid storage bucket 5, gu fixed ring 12 cover is in the outside of toper connecting cylinder 501, gu the outside of fixed ring 12 is provided with connecting plate 1201, and the below of connecting plate 1201 is provided with screw rod 1202, and the lower extreme of screw rod 1202 stretches out the base and passes through the nut fixed.
Example five:
on the basis of the first embodiment, the second embodiment, the third embodiment or the fourth embodiment, feed inlets controlled by valves are arranged on the containers 6, so that the culture solution is increased conveniently.
Example six:
on the basis of the first embodiment or the second embodiment or the third embodiment or the fourth embodiment or the fifth embodiment, the culture dish body 1 is circular, an annular boss is arranged at the lower edge of the culture dish body 1, and the cavity 101 is arranged on the annular boss and is in the shape of an annular groove.
Example seven:
on the basis of the first embodiment, the second embodiment, the third embodiment, the fourth embodiment, the fifth embodiment and the sixth embodiment, the container is a water bag made of a flexible material, and rubber is preferable.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings, which are merely for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be construed as limiting the invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, the meaning of "a plurality" is two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the creation of the present invention can be understood by those of ordinary skill in the art through specific situations.
The technical features of the present invention, which are not described in the present application, can be implemented by or using the prior art, and are not described herein again, and of course, the above description is not limited to the above examples, and the present invention is not limited to the above examples, and variations, modifications, additions and substitutions which can be made by those skilled in the art within the spirit of the present invention should also fall within the scope of the present invention.

Claims (7)

1. The device for culturing the animal muscle cells is characterized by comprising a culture dish body and a dish cover, wherein a sealed cavity is arranged at the lower part of the culture dish body, a liquid inlet pipe communicated with the cavity is arranged in the culture dish body, and a liquid outlet of the liquid inlet pipe is higher than the height of the liquid level in the culture dish body and the height of the liquid level in the cavity;
the culture dish further comprises a container, wherein the container is communicated with the cavity through a one-way valve, and the liquid storage space of the container can be adjusted;
the extrusion mechanism is used for controlling the liquid storage space in the container to be reduced;
the extruding mechanism comprises an extruding barrel and a first sealing piston arranged in the extruding barrel, and the container is arranged in the extruding barrel and below the first sealing piston;
the sealing device is characterized by further comprising a driving mechanism for controlling the first sealing piston, the driving mechanism comprises a liquid storage barrel connected with the extrusion barrel, fluid is arranged in the liquid storage barrel, the liquid storage barrel is communicated with the extrusion barrel through a fluid flow limiting structure, an exhaust pipe for exhausting air to the outside of the extrusion barrel is arranged on the extrusion barrel and above the first sealing piston, a second one-way valve is arranged on the exhaust pipe, the fluid gradually enters the extrusion barrel, and the first sealing piston moves downwards under the action of gravity of the fluid;
the fluid flow limiting structure is a flow limiting valve or a plurality of plugs I which are provided with water outlet holes, and the inner diameters of the water outlet holes on different plugs I are different;
the culture dish comprises a culture dish body and is characterized by further comprising a liquid drainage mechanism arranged below the culture dish body, wherein the liquid drainage mechanism comprises a liquid drainage barrel, the liquid drainage barrel is arranged below the culture dish body, a plurality of liquid drainage holes are formed in the culture dish body, an annular baffle is arranged below the culture dish body and on the periphery of the liquid drainage holes, a second plug for plugging the liquid drainage holes is arranged on the annular baffle, and the second plug is arranged below the culture dish body through a spring;
a second sealing piston is arranged in the liquid discharge barrel, a U-shaped pipe is externally connected to the upper portion of the side wall of the liquid discharge barrel, the upper outlet and the lower outlet of the U-shaped pipe are communicated with the inside of the liquid discharge barrel, a liquid discharge port is formed in the side wall of the liquid discharge barrel and located below the U-shaped pipe, the thickness of the second sealing piston is smaller than the minimum distance between the two outlets, and the liquid discharge mechanism is adjusted in advance to enable the sealing piston to be located between the two outlets;
the lower end of the liquid discharge barrel is sealed, a piston rod is concentrically arranged on the sealing piston II, the piston rod is arranged on the liquid discharge barrel through threaded connection, and when culture solution needs to be replaced or waste culture solution is discharged, the piston rod can be rotated to enable the sealing piston II to move downwards.
2. The apparatus for culturing animal muscle cells as claimed in claim 1, wherein the reservoir comprises a conical connecting cylinder at a lower portion, a cylindrical cylinder at an upper portion, and a connecting pipe which is connected to the conical connecting cylinder and the cylindrical cylinder and is contracted toward the axial center, and the fluid restriction structure is disposed on the connecting pipe.
3. The apparatus for culturing animal muscle cells according to claim 1, wherein the sealing piston is fixed to an upper portion of a sidewall of the pressing barrel by an elastic member.
4. The device for culturing animal muscle cells according to claim 1, wherein the container is a water bladder made of a flexible material.
5. The device for culturing animal muscle cells according to claim 1, wherein the container is integrally provided with the squeeze barrel, the bottom of the squeeze barrel is sealed by a bottom plate, and the container is arranged between the bottom plate of the squeeze barrel and the first sealing piston.
6. The device for culturing animal muscle cells according to claim 1, wherein the fluid is oil or water.
7. The device for culturing animal muscle cells of claim 1, wherein a plurality of liquid inlet pipes are uniformly distributed along the inner wall of the culture dish body, and the liquid inlet pipes are communicated with the cavity.
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