CN112552426B - Method and equipment for extracting heparin sodium in porcine small intestine mucosa - Google Patents
Method and equipment for extracting heparin sodium in porcine small intestine mucosa Download PDFInfo
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- CN112552426B CN112552426B CN202011474440.2A CN202011474440A CN112552426B CN 112552426 B CN112552426 B CN 112552426B CN 202011474440 A CN202011474440 A CN 202011474440A CN 112552426 B CN112552426 B CN 112552426B
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- 229920000669 heparin Polymers 0.000 title claims abstract description 38
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 title claims abstract description 36
- 229960001008 heparin sodium Drugs 0.000 title claims abstract description 36
- 210000000813 small intestine Anatomy 0.000 title claims abstract description 20
- 210000004877 mucosa Anatomy 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 75
- 238000003756 stirring Methods 0.000 claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 238000005192 partition Methods 0.000 claims abstract description 43
- 238000007789 sealing Methods 0.000 claims abstract description 32
- 238000001914 filtration Methods 0.000 claims abstract description 29
- 239000011347 resin Substances 0.000 claims abstract description 28
- 229920005989 resin Polymers 0.000 claims abstract description 28
- 238000004140 cleaning Methods 0.000 claims description 18
- 230000035939 shock Effects 0.000 claims description 7
- 239000000203 mixture Substances 0.000 abstract description 6
- 239000012266 salt solution Substances 0.000 abstract description 2
- 239000012045 crude solution Substances 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000011780 sodium chloride Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000003480 eluent Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 244000309464 bull Species 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 239000012043 crude product Substances 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 206010008132 Cerebral thrombosis Diseases 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 201000001429 Intracranial Thrombosis Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000002429 anti-coagulating effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/80—After-treatment of the mixture
- B01F23/808—Filtering the mixture
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/80—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations
- B01F31/83—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations comprising a supplementary stirring element
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/10—Maintenance of mixers
- B01F35/12—Maintenance of mixers using mechanical means
- B01F35/121—Maintenance of mixers using mechanical means using a brush for cleaning out rests of products
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/40—Mounting or supporting mixing devices or receptacles; Clamping or holding arrangements therefor
- B01F35/43—Supporting receptacles on frames or stands
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
- B01F2035/35—Use of other general mechanical engineering elements in mixing devices
- B01F2035/351—Sealings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/2204—Mixing chemical components in generals in order to improve chemical treatment or reactions, independently from the specific application
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Mechanical Engineering (AREA)
- Sustainable Development (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Filtration Of Liquid (AREA)
Abstract
The invention discloses a method and equipment for extracting heparin sodium from porcine small intestine mucosa, wherein a mixing shell of a supporting assembly is connected with a bottom shell, a first partition plate is arranged between the bottom shell and the mixing shell, a circulating pump is arranged on one side of the first partition plate, an ultrasonic generator and a stirring motor of a stirring assembly are connected with the mixing shell, stirring blades are connected with the stirring motor, a sealing plate is connected with the first partition plate through a supporting spring, a control rod is connected with the mixing shell in a sliding manner, a limiting piece is arranged on one side of the control rod in a sliding manner, a mounting plate of a filtering assembly is detachably connected with the bottom shell, and a plurality of resin plates are arranged on the mounting plate. Pour into heparin sodium crude and salt solution and start stirring vane in mixing the shell and mix, start supersonic generator and dissolve with higher speed, remove the slide bar and push open the closing plate and make mixed liquid get into the drain pan, drive at the drain pan mesocycle through the circulating pump to make a plurality of resin boards filter heparin sodium, filtering component has two sets ofly in order further to improve filtration efficiency, make and fully filter effective component.
Description
Technical Field
The invention relates to the field of biochemical raw material medicine production equipment, in particular to a method and equipment for extracting heparin sodium from porcine small intestine mucosa.
Background
Heparin is a mucopolysaccharide widely present in mammalian tissues, is mainly present in mast cells, has an anticoagulant effect, and is widely used in surgery and treatment of cerebral thrombosis, myocardial infarction and the like. Heparin sodium is the sodium salt of heparin. Heparin sodium is regarded as a natural anticoagulant substance by various countries in the world. Is also one of the main export drugs in China. With the progress of research, people find that the heparin sodium not only has the effects of anticoagulation, anti-thrombosis and blood fat regulation, but also has the functions of anti-inflammation, anti-allergy, anti-virus, anti-cancer and the like. At present, heparin sodium is mainly extracted from mucous membranes of small intestines of pigs and sheep, lung of cows, napping sausage casing saline and the like.
The existing heparin sodium has poor filtering effect after being dissolved, so that the material utilization rate is low, and the production efficiency is low.
Disclosure of Invention
The invention aims to provide a method and equipment for extracting heparin sodium from porcine small intestine mucosa, and aims to solve the problems that the existing equipment is poor in filtering effect and low in production efficiency.
In order to achieve the above object, in a first aspect, the present invention provides an apparatus for extracting heparin sodium from porcine small intestine mucosa, comprising: the device comprises a supporting component, a stirring component and a filtering component, wherein the supporting component comprises a mixing shell, a bottom shell, a first partition plate and a circulating pump, the mixing shell is provided with a liquid inlet, the bottom shell is provided with a first groove and a liquid outlet, the liquid outlet is positioned on one side of the first groove, the mixing shell is fixedly connected with the bottom shell and positioned in the first groove, the first partition plate is provided with a second groove, the first partition plate is fixedly connected with the bottom shell and positioned on one side of the mixing shell, the circulating pump is fixedly connected with the bottom shell and positioned on one side of the first partition plate far away from the mixing shell, the stirring component comprises an ultrasonic generator, a stirring motor, stirring blades, a sealing plate, a supporting spring, a control rod and a limiting piece, the ultrasonic generator is fixedly connected with the mixing shell and positioned on one side of the mixing shell, the stirring motor is fixedly connected with the mixing shell and positioned at one side of the liquid inlet, the stirring blade is fixedly connected with the output end of the stirring motor and positioned in the mixing shell, the sealing plate is slidably connected with the first partition plate and contacts with the mixing shell, the supporting spring is fixedly connected with the first partition plate and positioned between the sealing plate and the first partition plate and positioned in the second groove, the control rod is slidably connected with the mixing shell and penetrates through the mixing shell, the limiting piece is slidably connected with the mixing shell and positioned at one side of the control rod, the filtering component comprises a mounting plate and a plurality of resin plates, the mounting plate is detachably connected with the bottom shell and penetrates through the bottom shell, the plurality of resin plates are fixedly connected with the mounting plate and positioned in the bottom shell, and the number of the filtering components is two, respectively located on both sides of the mixing shell.
The supporting assembly further comprises a shock pad, and the shock pad is fixedly connected with the bottom shell and is located on one side, away from the first groove, of the bottom shell.
Wherein, stirring vane includes blade body, extension rod, clearance pole and cleaning brush, the extension rod with blade body fixed connection to be located one side of blade body, the clearance pole with the extension rod rotates to be connected, the extension rod passes the clearance pole, the cleaning brush with clearance pole fixed connection, and be located the clearance pole is kept away from one side of extension rod.
Wherein, spacing piece includes lamellar body, spacing spring and handle, spacing spring with lamellar body fixed connection to be located the lamellar body with between the drain pan, the handle with lamellar body fixed connection, and be located one side of lamellar body.
The filter assembly further comprises a sealing ring, wherein the sealing ring is fixedly connected with the mounting plate and is positioned on one side of the mounting plate close to the resin plate.
The filter assembly further comprises a second partition plate, and the second partition plate is fixedly connected with the mounting plate and is positioned between every two resin plates.
In a second aspect, the invention provides a method for extracting heparin sodium from porcine small intestine mucosa, which comprises the following steps: putting the prepared heparin sodium crude product and saline water through a liquid inlet; starting the stirring blade to stir and mix the materials; after mixing, the stirring motor is turned off, and the control rod is pushed to enable the mixed liquid to enter the bottom shell; starting a circulating pump to circulate the mixed solution in the bottom shell, and filtering the mixed solution through the resin plate; collecting the eluent in the bottom case, adding alcohol for precipitation, and freeze-drying to obtain the final product.
The invention relates to a method and equipment for extracting heparin sodium in porcine small intestine mucosa, wherein a crude product of heparin sodium and saline water are prepared and input from a liquid inlet, a first groove is used as a main filtering place, eluent which is processed is discharged from a liquid outlet after the filtering is finished and is processed in the next step, a mixing shell is fixedly connected with a bottom shell, a first partition plate is fixedly connected with the bottom shell, a circulating pump is fixedly connected with the bottom shell, the liquid in the bottom shell can be rotated through the circulating pump, so that the filtering rate is improved, a stirring motor is fixedly connected with the mixing shell, a stirring blade is fixedly connected with the output end of the stirring motor, the stirring blade can be driven to rotate through the stirring motor, a sealing plate is slidably connected with the first partition plate, and a supporting spring is fixedly connected with the first partition plate, the first partition plate can enable the sealing plate to seal the mixing shell under the action of the supporting spring while partitioning the liquid in the bottom shell, so that the raw material and the saline water can be premixed in the mixing shell, the control rod is connected with the mixing shell in a sliding mode, the limiting piece is connected with the mixing shell in a sliding mode, after the mixing is completed, the stirring blades are closed, the control rod is controlled to move downwards to push open the sealing plate, so that the mixed liquid can enter the bottom shell, then the position of the control rod is limited through the limiting piece, the mounting plate is detachably connected with the bottom shell, the resin plates are fixedly connected with the mounting plate, the resin plates are used for filtering the heparin sodium component and are uniformly mounted on the mounting plate, and multi-layer filtering can be performed, the filter efficiency can be improved, the number of the filter assemblies is two groups, the filter assemblies are respectively positioned on two sides of the mixing shell and two groups of the resin plates can further improve the filter efficiency, so that effective components in the mixed liquid can be fully filtered, the problem that the filter effect of the existing equipment is poor and the production efficiency is reduced is solved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a front structural view of an apparatus for extracting heparin sodium from porcine small intestine mucosa according to the present invention;
FIG. 2 is a schematic cross-sectional view of FIG. 1;
FIG. 3 is a rear structure diagram of an apparatus for extracting heparin sodium from porcine small intestine mucosa according to the present invention;
FIG. 4 is a flow chart of the method for extracting heparin sodium from porcine small intestine mucosa according to the invention.
1-supporting component, 2-stirring component, 3-filtering component, 4-rotating rod, 5-handle, 11-mixing shell, 12-bottom shell, 13-first clapboard, 14-circulating pump, 15-shock pad, 16-limiting column, 20-ultrasonic generator, 21-stirring motor, 22-stirring blade, 23-sealing plate, 24-supporting spring, 25-control rod, 26-limiting sheet, 31-mounting plate, 32-resin plate, 33-sealing ring, 34-second clapboard, 111-liquid inlet, 121-first groove, 122-liquid outlet, 131-second groove, 221-blade body, 222-extension rod, 223-cleaning rod, 224-cleaning brush, 261-sheet body, 262-limit spring, 263-handle.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 3, in a first aspect, the present invention provides an apparatus for extracting heparin sodium from porcine small intestine mucosa, including:
the stirring device comprises a supporting component 1, a stirring component 2 and a filtering component 3, wherein the supporting component 1 comprises a mixing shell 11, a bottom shell 12, a first partition plate 13 and a circulating pump 14, the mixing shell 11 is provided with a liquid inlet 111, the bottom shell 12 is provided with a first groove 121 and a liquid outlet 122, the liquid outlet 122 is positioned on one side of the first groove 121, the mixing shell 11 is fixedly connected with the bottom shell 12 and positioned in the first groove 121, the first partition plate 13 is provided with a second groove 131, the first partition plate 13 is fixedly connected with the bottom shell 12 and positioned on one side of the mixing shell 11, the circulating pump 14 is fixedly connected with the bottom shell 12 and positioned on one side of the first partition plate 13 far away from the mixing shell 11, the stirring component 2 comprises an ultrasonic generator 20, a stirring motor 21, a stirring blade 22, a sealing plate 23, a supporting spring 24, a control rod 25 and a limiting sheet 26, the ultrasonic generator 20 is fixedly connected to the mixing casing 11 and located at one side of the mixing casing 11, the stirring motor 21 is fixedly connected to the mixing casing 11 and located at one side of the liquid inlet 111, the stirring blade 22 is fixedly connected to an output end of the stirring motor 21 and located in the mixing casing 11, the sealing plate 23 is slidably connected to the first partition plate 13 and contacts the mixing casing 11, the supporting spring 24 is fixedly connected to the first partition plate 13 and located between the sealing plate 23 and the first partition plate 13 and located in the second groove 131, the control rod 25 is slidably connected to the mixing casing 11 and passes through the mixing casing 11, the limiting piece 26 is slidably connected to the mixing casing 11 and located at one side of the control rod 25, the filter assembly 3 includes a mounting plate 31 and a plurality of resin plates 32, the mounting panel 31 with the drain pan 12 can be dismantled and be connected, and pass the drain pan 12, it is a plurality of resin plate 32 with mounting panel 31 fixed connection, and be located in the drain pan 12, there are two sets of filter assembly 3's quantity, are located respectively the both sides of mixing shell 11.
In this embodiment, the supporting assembly 1 includes a mixing shell 11, a bottom shell 12, a first partition plate 13 and a circulating pump 14, the mixing shell 11 has a liquid inlet 111 through which crude heparin sodium and saline are prepared and fed, the bottom shell 12 has a first groove 121 and a liquid outlet 122, the liquid outlet 122 is located at one side of the first groove 121, the first groove 121 is used as a main filtering place, eluent after the preparation is filtered and discharged from the liquid outlet 122 for further processing, the mixing shell 11 is fixedly connected with the bottom shell 12 and located in the first groove 121, the first partition plate 13 has a second groove 131, the first partition plate 13 is fixedly connected with the bottom shell 12 and located at one side of the mixing shell 11, the circulating pump 14 is fixedly connected with the bottom shell 12 and located at one side of the first partition plate 13 far from the mixing shell 11, the liquid in the bottom shell 12 can be rotated by the circulating pump 14, so as to improve the filtration rate, the stirring assembly 2 includes an ultrasonic generator 20, a stirring motor 21, a stirring blade 22, a sealing plate 23, a support spring 24, a control rod 25 and a limiting piece 26, the ultrasonic generator 20 is fixedly connected with the mixing shell 11 and is located at one side of the mixing shell 11, and can generate ultrasonic waves to act on the liquid to generate cavitation, thermal effect and give molecular momentum to the liquid, so as to improve the dissolution rate, the stirring motor 21 is fixedly connected with the mixing shell 11 and is located at one side of the liquid inlet 111, the stirring blade 22 is fixedly connected with the output end of the stirring motor 21 and is located in the mixing shell 11, the stirring motor 21 can drive the stirring blade 22 to rotate, and the sealing plate 23 is slidably connected with the first partition plate 13, and contacting the mixing shell 11, the supporting spring 24 is fixedly connected with the first partition plate 13, and is located between the sealing plate 23 and the first partition plate 13, and is located in the second groove 131, the first partition plate 13 can divide the liquid in the bottom shell 12, and at the same time, the sealing plate 23 can seal the mixing shell 11 under the action of the supporting spring 24, so that the preparation raw material and the saline water can be premixed in the mixing shell 11, the control rod 25 is slidably connected with the mixing shell 11 and passes through the mixing shell 11, the limiting piece 26 is slidably connected with the mixing shell 11 and is located at one side of the control rod 25, after the mixing is completed, the stirring blade 22 is closed, the control rod 25 is controlled to move downwards to push the sealing plate 23 open, so that the mixed liquid can enter the bottom shell 12, then pass through spacing piece 26 is right the position of control lever 25 is restricted, filtering component 3 includes mounting panel 31 and a plurality of resin board 32, mounting panel 31 with drain pan 12 can dismantle the connection, and passes drain pan 12, and is a plurality of resin board 32 with mounting panel 31 fixed connection, and lie in the drain pan 12, resin board 32 is used for exchanging impurity composition, installs in unison on the mounting panel 31, can carry out the multistage filtration for can improve and filter exchange efficiency, filtering component 3's quantity has two sets ofly, lies in respectively mix the both sides of shell 11, and is two sets of resin board 32 can further improve filtration efficiency, makes can fully filter the active ingredient in the mixed liquid, thereby solves the problem that current equipment filter effect is not good, makes production efficiency reduce.
Further, the support assembly 1 further includes a shock pad 15, where the shock pad 15 is fixedly connected to the bottom case 12 and is located on a side of the bottom case 12 away from the first groove 121.
In this embodiment, the shock pad 15 is disposed on one side of the bottom case 12 to reduce vibration received during operation, so that the operation can be more stable.
Further, the stirring blade 22 includes a blade body 221, an extension rod 222, a cleaning rod 223 and a cleaning brush 224, the extension rod 222 is fixedly connected to the blade body 221 and located on one side of the blade body 221, the cleaning rod 223 is rotatably connected to the extension rod 222, the extension rod 222 passes through the cleaning rod 223, and the cleaning brush 224 is fixedly connected to the cleaning rod 223 and located on one side of the cleaning rod 223 away from the extension rod 222.
In this embodiment, the blade body 221 is supported by the stirring motor 21 to rotate, the cleaning rod 223 is supported by the extension rod 222 to drive the cleaning brush 224 to rotate so as to clean the side wall of the mixing shell 11, and the extension rod 222 and the cleaning rod 223 are driven by friction force, so that the rotation speed of the cleaning brush 224 can be limited, and the phenomenon that the rotation speed is too fast and the energy consumption is too high is avoided.
Further, the limiting piece 26 includes a sheet body 261, a limiting spring 262 and a handle 263, the limiting spring 262 is fixedly connected to the sheet body 261 and is located between the sheet body 261 and the bottom case 12, and the handle 263 is fixedly connected to the sheet body 261 and is located on one side of the sheet body 261.
In this embodiment, the blade 261 can slide relative to the bottom case 12 under the action of the limiting spring 262, so that after the control rod 25 abuts against the sealing plate 23 downwards, the position of the control rod 25 can be supported to keep the sealing plate 23 in the open position, and when the stirring needs to be recharged, the handle 263 retracts the blade 261, so that the control rod 25 is lifted, and the sealing plate 23 returns to the sealing position again.
Further, the filter assembly 3 further includes a sealing ring 33, wherein the sealing ring 33 is fixedly connected to the mounting plate 31 and is located on one side of the mounting plate 31 close to the resin plate 32.
In the present embodiment, the packing 33 is disposed at one side of the mounting plate 31, so that the sealing performance of the joint can be increased after the mounting plate 31 is coupled to the bottom case 12, thereby preventing leakage during operation.
Further, the filter assembly 3 further includes a second partition plate 34, and the second partition plate 34 is fixedly connected to the mounting plate 31 and is located between each two resin plates 32.
In the present embodiment, the second partition 34 is provided between the two resin plates 32, so that it is possible to avoid direct backflow of the liquid from a position between the resin plates 32, thereby increasing the contact time of the resin plates 32 and the liquid, and thus improving the filtration efficiency.
Further, extract heparin sodium equipment in pig small intestine mucosa still includes two bull sticks 4 and handle 5, two bull stick 4 and two the mounting panel 31 rotates to be connected, and is located respectively one side of mounting panel 31, handle 5 and two 4 sliding connection of bull stick, and be located one side of bull stick 4.
In this embodiment, the two rotating rods 4 are disposed on one side of the mounting plate 31, so that the handle 5 can rotate relative to the mounting plate 31 under the support of the rotating rods 4, and the two mounting plates 31 can be conveniently detached and installed simultaneously, and can also rotate to reduce the occupied space.
Further, the supporting assembly 1 further includes a limiting post 16, and the limiting post 16 is fixedly connected to the bottom case 12 and is located on one side of the bottom case 12 close to the handle 5.
In this embodiment, the position-limiting column 16 is disposed on one side of the bottom case 12, so that the handle 5 can be rotated, and the handle 5 slides relative to the rotating rod 4 to enter the position-limiting column 16, so as to be pressed by the position-limiting column 16, thereby pressing and fixing the mounting plate 31, and avoiding leakage during operation.
In a second aspect, referring to fig. 4, the present invention further provides a method for extracting heparin sodium from porcine small intestine mucosa, comprising:
s101, preparing a heparin sodium crude product and saline water through a liquid inlet 111;
the crude heparin sodium product and saline are prepared according to proper material proportion, so that the crude heparin sodium product and the saline can be mixed, and the heparin sodium can be dissolved in the saline.
S102, starting the stirring blade 22 to stir and mix the materials;
stirring vane 22 can carry out abundant mixture to the material for heparin sodium can fully dissolve in the salt solution, can add cleaning brush 224 on stirring vane 22 in addition and clear up the lateral wall, thereby improves the utilization ratio of material.
S103, after the mixing is finished, the stirring motor 21 is turned off, and the control rod 25 is pushed to enable the mixed liquid to enter the bottom shell 12;
after the stirring blade 22 stops rotating, the control rod 25 slides so that the sealing plate 23 can be covered to make the mixed liquid enter the bottom case 12, and the position of the control rod 25 can be limited and maintained by the limiting piece 26.
S104, starting the circulating pump 14 to circulate the mixed liquid in the bottom shell 12, and filtering the mixed liquid through the resin plate 32;
the circulation pump 14 may circulate the mixed liquid in the bottom case 12 continuously to be filtered by the resin plate 32, so that the filtering efficiency may be improved.
S105, collecting the eluent in the bottom case 12, adding alcohol for precipitation, and freeze-drying to obtain a finished product.
After filtering, collecting eluent from the liquid outlet, adding alcohol for precipitation, and finally obtaining the heparin sodium after ultrafiltration and freeze-drying.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (5)
1. An apparatus for extracting heparin sodium in porcine small intestine mucosa is characterized in that,
the device comprises a supporting component, a stirring component and a filtering component, wherein the supporting component comprises a mixing shell, a bottom shell, a first partition plate and a circulating pump, the mixing shell is provided with a liquid inlet, the bottom shell is provided with a first groove and a liquid outlet, the liquid outlet is positioned on one side of the first groove, the mixing shell is fixedly connected with the bottom shell and positioned in the first groove, the first partition plate is provided with a second groove, the first partition plate is fixedly connected with the bottom shell and positioned on one side of the mixing shell, the circulating pump is fixedly connected with the bottom shell and positioned on one side of the first partition plate far away from the mixing shell, the stirring component comprises an ultrasonic generator, a stirring motor, stirring blades, a sealing plate, a supporting spring, a control rod and a limiting piece, the ultrasonic generator is fixedly connected with the mixing shell and positioned on one side of the mixing shell, the stirring motor is fixedly connected with the mixing shell and positioned at one side of the liquid inlet, the stirring blade is fixedly connected with the output end of the stirring motor and positioned in the mixing shell, the sealing plate is slidably connected with the first partition plate and contacts with the mixing shell, the supporting spring is fixedly connected with the first partition plate and positioned between the sealing plate and the first partition plate and positioned in the second groove, the control rod is slidably connected with the mixing shell and penetrates through the mixing shell, the limiting piece is slidably connected with the mixing shell and positioned at one side of the control rod, the filtering component comprises a mounting plate and a plurality of resin plates, the mounting plate is detachably connected with the bottom shell and penetrates through the bottom shell, the plurality of resin plates are fixedly connected with the mounting plate and positioned in the bottom shell, and the number of the filtering components is two, be located respectively the both sides of mixing the shell, stirring vane includes blade body, extension rod, clearance pole and cleaning brush, the extension rod with blade body fixed connection to be located one side of blade body, the clearance pole with the extension rod rotates to be connected, the extension rod passes the clearance pole, the cleaning brush with clearance pole fixed connection, and be located the clearance pole is kept away from one side of extension rod.
2. The device for extracting the heparin sodium in the porcine small intestine mucosa as claimed in claim 1,
the supporting assembly further comprises a shock pad, and the shock pad is fixedly connected with the bottom shell and is located on one side, away from the first groove, of the bottom shell.
3. The device for extracting the heparin sodium in the porcine small intestine mucosa as claimed in claim 1,
the limiting piece comprises a piece body, a limiting spring and a handle, the limiting spring is fixedly connected with the piece body and is positioned between the piece body and the bottom shell, and the handle is fixedly connected with the piece body and is positioned on one side of the piece body.
4. The device for extracting the heparin sodium in the porcine small intestine mucosa as claimed in claim 1,
the filter assembly further comprises a sealing ring, wherein the sealing ring is fixedly connected with the mounting plate and is positioned at one side of the mounting plate close to the resin plate.
5. The device for extracting the heparin sodium in the porcine small intestine mucosa as claimed in claim 4,
the filter assembly further comprises a second partition plate, wherein the second partition plate is fixedly connected with the mounting plate and is positioned between every two resin plates.
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| CN202011474440.2A CN112552426B (en) | 2020-12-14 | 2020-12-14 | Method and equipment for extracting heparin sodium in porcine small intestine mucosa |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011474440.2A CN112552426B (en) | 2020-12-14 | 2020-12-14 | Method and equipment for extracting heparin sodium in porcine small intestine mucosa |
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| CN112552426A CN112552426A (en) | 2021-03-26 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105001353A (en) * | 2015-08-17 | 2015-10-28 | 江苏联众肠衣有限公司 | Refining optimization technology for crude heparin sodium |
| CN106589167A (en) * | 2016-11-29 | 2017-04-26 | 重庆伊诺生化制品有限公司 | Preparation method of high-titer heparin sodium crude product and production apparatus therefor |
| CN206701272U (en) * | 2017-05-05 | 2017-12-05 | 连云港恒飞制药有限公司 | A kind of multistage purification devices of medicine intermediate |
| CN108359029A (en) * | 2018-04-25 | 2018-08-03 | 南京凯扬生物科技有限公司 | A kind of technique and device of extraction purification heparin |
| CN212998552U (en) * | 2020-07-09 | 2021-04-20 | 固镇县环宇食品有限公司 | Crude heparin sodium liquid precipitation separation device |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105001353A (en) * | 2015-08-17 | 2015-10-28 | 江苏联众肠衣有限公司 | Refining optimization technology for crude heparin sodium |
| CN106589167A (en) * | 2016-11-29 | 2017-04-26 | 重庆伊诺生化制品有限公司 | Preparation method of high-titer heparin sodium crude product and production apparatus therefor |
| CN206701272U (en) * | 2017-05-05 | 2017-12-05 | 连云港恒飞制药有限公司 | A kind of multistage purification devices of medicine intermediate |
| CN108359029A (en) * | 2018-04-25 | 2018-08-03 | 南京凯扬生物科技有限公司 | A kind of technique and device of extraction purification heparin |
| CN212998552U (en) * | 2020-07-09 | 2021-04-20 | 固镇县环宇食品有限公司 | Crude heparin sodium liquid precipitation separation device |
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| CN112552426A (en) | 2021-03-26 |
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Denomination of invention: A Method and Equipment for Extracting Sodium Heparin from Pig Small Intestinal Mucosa Effective date of registration: 20231009 Granted publication date: 20220318 Pledgee: Bank of Nanjing Co.,Ltd. Yancheng branch Pledgor: JIANGSU WANLI BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023980060418 |
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Address after: 224200 Jiangsu Yancheng City Dongtai coastal economic zone port area two road 20 Patentee after: Jiangsu Wanli Biotechnology Co.,Ltd. Country or region after: China Address before: No.20, gangang 2nd Road, Dongtai coastal economic zone, Yancheng City, Jiangsu Province Patentee before: JIANGSU WANLI BIOTECHNOLOGY Co.,Ltd. Country or region before: China |
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