CN112544731A - Preparation process of antarctic krill oil nanoemulsion - Google Patents
Preparation process of antarctic krill oil nanoemulsion Download PDFInfo
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- CN112544731A CN112544731A CN202011609197.0A CN202011609197A CN112544731A CN 112544731 A CN112544731 A CN 112544731A CN 202011609197 A CN202011609197 A CN 202011609197A CN 112544731 A CN112544731 A CN 112544731A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D7/00—Edible oil or fat compositions containing an aqueous phase, e.g. margarines
- A23D7/02—Edible oil or fat compositions containing an aqueous phase, e.g. margarines characterised by the production or working-up
- A23D7/04—Working-up
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Abstract
The invention relates to the technical field of krill oil, in particular to a preparation process of antarctic krill oil nanoemulsion, wherein the preparation process is carried out by utilizing an oil phase and a water phase and adopting a preparation system comprising an improved static mixer, the emulsion prepared by the method has good particle size uniformity, and the emulsification yield is more than 95%.
Description
Technical Field
The invention relates to the technical field of krill oil, and particularly relates to a preparation process of antarctic krill oil nanoemulsion.
Background
The antarctic krill oil is used as pure natural oil extracted from the antarctic krill, and is rich in nutritional ingredients; a large number of studies at home and abroad prove that the compound has various biological activity functions. Its active functions include preventing cardiovascular diseases and improving premenstrual syndrome. Due to the characteristics of weak chemical stability, easy oxidation and water insolubility, the application in the food industry is greatly limited. In the current food industry, antarctic krill oil is mainly prepared into soft capsules to be produced as health care products.
For the industrial production of antarctic krill oil, no better solution for better application in the video industry using antarctic krill oil is proposed at present. For example, some researchers have prepared the krill peptide into microcapsules, and the form of the krill peptide limits the shape of the product, and the preparation process is complex and cannot be applied to the production of the food industry on a large scale.
Based on this, there is a need to design a way for antarctic krill oil suitable for industrial production for use in the food industry.
Disclosure of Invention
The invention aims to provide a preparation process of antarctic krill oil nanoemulsion, which is used for solving the problems existing in the current application of krill oil in the food industry.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a preparation process of antarctic krill oil nanoemulsion comprises the following steps:
step 1, adding 1-3 parts by weight of sodium starch octenyl succinate and 3-5 parts by weight of carboxymethyl cellulose into 100 parts by weight of phosphate buffer, and simultaneously adding a water-based antioxidant and an emulsifier to obtain a water phase;
step 2, dissolving the antarctic krill oil in the mixed organic solvent, wherein the concentration of the antarctic krill oil in the solution is 10-20% ml, and obtaining an oil phase; the mixed solvent is ethanol and acetone, and the volume ratio of the ethanol to the acetone is 3-8: 1;
and 4, removing the organic solvent in the nano emulsion under a vacuum state to obtain the nano emulsion.
Preferably, in step 3, the temperature is heated to 40 ℃ to 60 ℃ before stirring.
Preferably, the volume ratio of ethanol to acetone is 3-5: 1.
Preferably, the preparation system adopted in the step 3 comprises an oil phase tank and a water phase tank, wherein the oil phase tank and the water phase tank are provided with a metering pump through pipelines, and the rear part of the metering pump is connected with a stirring tank and an ultrasonic processor and then is connected with a static mixer through the metering pump; a buffer tank is arranged in front of the static mixer, and a constant pressure tank is connected with the buffer tank.
The oil phase tank and the water phase tank are used for storing an oil phase and a water phase respectively, the metering pump is used for accurately controlling the proportion and the flow rate of the oil phase and the water phase, the oil phase and the water phase enter the stirring tank from ports to be stirred and treated and are subjected to ultrasonic treatment under the driving of the metering pump, then the flow rate is accurately controlled through the metering pump to enter the static mixer, the emulsification is further stabilized in the oil phase and the water phase, and then the emulsified liquid enters a rear-end pipeline with a narrowed pipe diameter, and uniform and controllable emulsion droplets are formed under the action of pressure. The metering pump has fluctuation, and the influence of the fluctuation of the metering pump on the emulsification process is mainly reflected in the emulsification particle size and the emulsification uniformity; therefore, in order to reduce the influence of the metering pump on the emulsification process as much as possible, a buffer tank is arranged in front of the static mixer, and a constant pressure tank is connected to the buffer tank. The fluctuation of the metering pump is further reduced by utilizing the buffer tank, and the influence of the metering pump on the emulsification process is avoided.
Preferably, the lower part of the buffer tank is provided with a liquid inlet pipe and a liquid outlet pipe, the buffer tank is filled with coarse emulsion, the upper part of the buffer tank is filled with buffer gas, and the constant-pressure tank is communicated with the top of the buffer tank.
The invention has the beneficial effects that: the nano emulsion prepared by the method has the average particle size of 80-120 nanometers, the uniformity is good, and the emulsification yield of the antarctic krill oil is not less than 95%.
Drawings
FIG. 1 is a schematic view showing the structure of a production system of the present invention.
In the figure: 1 oil phase tank, 2 water phase tank, 3 metering pump, 4 stirring tank, 5 ultrasonic processor, 6 static mixer, 7 buffer tank and 8 constant pressure tank.
Detailed Description
Further refinements will now be made on the basis of the representative embodiment shown in the figures. It should be understood that the following description is not intended to limit the embodiments to one preferred embodiment. On the contrary, it is intended to cover alternatives, modifications, and equivalents as may be included within the spirit and scope of the embodiments as defined by the appended claims.
In the following detailed description, reference is made to the accompanying drawings which form a part hereof, and in which is shown by way of illustration specific embodiments in accordance with the embodiments. Although these embodiments are described in sufficient detail to enable those skilled in the art to practice the embodiments, it is to be understood that these examples are not limiting, such that other examples may be used and that corresponding modifications may be made without departing from the spirit and scope of the embodiments.
Specifically, the application provides a preparation process of antarctic krill oil nanoemulsion, which comprises the following steps:
step 1, adding 1-3 parts by weight of sodium starch octenyl succinate and 3-5 parts by weight of carboxymethyl cellulose into 100 parts by weight of phosphate buffer, and simultaneously adding a water-based antioxidant and an emulsifier to obtain a water phase;
step 2, dissolving the antarctic krill oil in the mixed organic solvent, wherein the concentration of the antarctic krill oil in the solution is 10-20% ml, and obtaining an oil phase; the mixed solvent is ethanol and acetone, and the volume ratio of the ethanol to the acetone is 3-8: 1; preferably, the volume ratio of the ethanol to the acetone is 3-5: 1;
and 4, removing the organic solvent in the nano emulsion under a vacuum state to obtain the nano emulsion.
Referring to fig. 1, fig. 1 shows a preparation system used in step 3, as shown in fig. 1, comprising an oil phase tank and a water phase tank, wherein the oil phase tank and the water phase tank are provided with a metering pump through a pipeline, the rear part of the metering pump is connected with a stirring tank and an ultrasonic processor, and then is connected with a static mixer through the metering pump; a buffer tank is arranged in front of the static mixer, and a constant pressure tank is connected with the buffer tank. The lower part of the buffer tank is provided with a liquid inlet pipe and a liquid outlet pipe, the buffer tank is filled with coarse emulsion, the upper part of the buffer tank is filled with buffer gas, and the constant pressure tank is communicated with the top of the buffer tank.
This system, at first adopt the buffer tank to carry out the partial elimination to the unstability of measuring pump to, it is further, utilize the buffer tank to connect the constant voltage jar and carry out the constant voltage processing in to the buffer tank, even when the measuring pump is in undulant millet, the buffer tank still can utilize the pressure of constant voltage jar to carry the remaining liquid in the buffer tank to follow-up, further assurance like this can get into the stability of the liquid in the static mixer, thereby guaranteed the homogeneity of the emulsion that the static mixer prepared.
Example 1: a preparation process of antarctic krill oil nanoemulsion comprises the following steps:
step 1, adding 1 part of sodium starch octenyl succinate and 4 parts of carboxymethyl cellulose into 100 parts of phosphate buffer solution according to parts by weight, and simultaneously adding a water-based antioxidant and an emulsifier to obtain a water phase;
step 2, dissolving the antarctic krill oil in the mixed organic solvent, wherein the concentration of the antarctic krill oil in the solution is 20% ml, and obtaining an oil phase; the mixed solvent is ethanol and acetone, and the volume ratio of the ethanol to the acetone is 3: 1;
and 4, removing the organic solvent in the nano emulsion under a vacuum state to obtain the nano emulsion.
The detection shows that the average grain diameter of the emulsion is 80-100 nanometers, and the conversion rate is 96 percent.
Example 2: a preparation process of antarctic krill oil nanoemulsion comprises the following steps:
step 1, adding 2 parts by weight of sodium starch octenyl succinate and 5 parts by weight of carboxymethyl cellulose into 100 parts by weight of phosphate buffer solution, and simultaneously adding a water-based antioxidant and an emulsifier to obtain a water phase;
step 2, dissolving the antarctic krill oil in the mixed organic solvent, wherein the concentration of the antarctic krill oil in the solution is 10% ml, and obtaining an oil phase; the mixed solvent is ethanol and acetone, and the volume ratio of the ethanol to the acetone is 5: 1;
and 4, removing the organic solvent in the nano emulsion under a vacuum state to obtain the nano emulsion.
The detection shows that the average grain diameter of the emulsion is 90-120 nanometers, and the conversion rate is 96 percent.
Example 3: a preparation process of antarctic krill oil nanoemulsion comprises the following steps:
step 1, adding 3 parts by weight of sodium starch octenyl succinate and 3 parts by weight of carboxymethyl cellulose into 100 parts by weight of phosphate buffer solution, and simultaneously adding a water-based antioxidant and an emulsifier to obtain a water phase;
step 2, dissolving the antarctic krill oil in the mixed organic solvent, wherein the concentration of the antarctic krill oil in the solution is 10% ml, and obtaining an oil phase; the mixed solvent is ethanol and acetone, and the volume ratio of the ethanol to the acetone is 8: 1;
and 4, removing the organic solvent in the nano emulsion under a vacuum state to obtain the nano emulsion.
The detection shows that the average particle size of the emulsion is 100-120 nm, and the conversion rate is 95%.
For purposes of explanation, specific nomenclature is used in the above description to provide a thorough understanding of the described embodiments. It will be apparent, however, to one skilled in the art that these specific details are not required in order to practice the embodiments described above. Thus, the foregoing descriptions of specific embodiments described herein are presented for purposes of illustration and description. It is not intended to be exhaustive or to limit the embodiments to the precise form disclosed. It will be apparent to those skilled in the art that certain modifications, combinations, and variations can be made in light of the above teachings.
Claims (5)
1. A preparation process of antarctic krill oil nanoemulsion is characterized by comprising the following steps: the method comprises the following steps:
step 1, adding 1-3 parts by weight of sodium starch octenyl succinate and 3-5 parts by weight of carboxymethyl cellulose into 100 parts by weight of phosphate buffer, and simultaneously adding a water-based antioxidant and an emulsifier to obtain a water phase;
step 2, dissolving the antarctic krill oil in the mixed organic solvent, wherein the concentration of the antarctic krill oil in the solution is 10-20% ml, and obtaining an oil phase; the mixed solvent is ethanol and acetone, and the volume ratio of the ethanol to the acetone is 3-8: 1;
step 3, metering the oil phase and the water phase prepared in the step 1 and the step 2 by a metering pump, mixing, homogenizing and stirring for 5-10min, and performing ultrasonic dispersion, wherein the ultrasonic dispersion is to perform ultrasonic treatment for 10-20 min by using an ultrasonic cell crusher to obtain a coarse emulsion; then processing the crude emulsion by a static processor;
and 4, removing the organic solvent in the nano emulsion under a vacuum state to obtain the nano emulsion.
2. The process for preparing the antarctic krill oil nanoemulsion as claimed in claim 1, wherein the process comprises the following steps: in step 3, the temperature is first heated to 40-60 ℃ before stirring.
3. The process for preparing the antarctic krill oil nanoemulsion as claimed in claim 1, wherein the process comprises the following steps: the volume ratio of the ethanol to the acetone is 3-5: 1.
4. The process for preparing the antarctic krill oil nanoemulsion as claimed in claim 1, wherein the process comprises the following steps: the preparation system adopted in the step 3 comprises an oil phase tank and a water phase tank, wherein the oil phase tank and the water phase tank are provided with a metering pump through pipelines, and the rear part of the metering pump is connected with a stirring tank and an ultrasonic processor and then is connected with a static mixer through the metering pump; a buffer tank is arranged in front of the static mixer, and a constant pressure tank is connected with the buffer tank.
5. The process for preparing the antarctic krill oil nanoemulsion as claimed in claim 1, wherein the process comprises the following steps: the lower part of the buffer tank is provided with a liquid inlet pipe and a liquid outlet pipe, the buffer tank is filled with coarse emulsion, the upper part of the buffer tank is filled with buffer gas, and the constant pressure tank is communicated with the top of the buffer tank.
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| CN202011609197.0A CN112544731A (en) | 2020-12-30 | 2020-12-30 | Preparation process of antarctic krill oil nanoemulsion |
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| CN202011609197.0A CN112544731A (en) | 2020-12-30 | 2020-12-30 | Preparation process of antarctic krill oil nanoemulsion |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860506A (en) * | 2012-08-08 | 2013-01-09 | 辽宁省大连海洋渔业集团公司 | Krill oil microcapsual powder and its preparation method |
| CN203990558U (en) * | 2014-08-19 | 2014-12-10 | 随州市世力达生物科技有限公司 | A kind of preparation system of microcapsules pulvis |
| CN107874257A (en) * | 2017-11-09 | 2018-04-06 | 大连工业大学 | The preparation method of high embedding rate small particle antarctic krill oil nanoparticle |
| CN110338235A (en) * | 2019-07-03 | 2019-10-18 | 浙江工业大学 | A kind of preparation process loading flax oil nano emulsions |
-
2020
- 2020-12-30 CN CN202011609197.0A patent/CN112544731A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860506A (en) * | 2012-08-08 | 2013-01-09 | 辽宁省大连海洋渔业集团公司 | Krill oil microcapsual powder and its preparation method |
| CN203990558U (en) * | 2014-08-19 | 2014-12-10 | 随州市世力达生物科技有限公司 | A kind of preparation system of microcapsules pulvis |
| CN107874257A (en) * | 2017-11-09 | 2018-04-06 | 大连工业大学 | The preparation method of high embedding rate small particle antarctic krill oil nanoparticle |
| CN110338235A (en) * | 2019-07-03 | 2019-10-18 | 浙江工业大学 | A kind of preparation process loading flax oil nano emulsions |
Non-Patent Citations (1)
| Title |
|---|
| 温祖谋 等: "《制革工艺及材料学》", 轻工业出版社, pages: 490 - 491 * |
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