CN112516249A - Preparation method and application of gorgeous leaf juice or gorgeous leaf powder with complete and full-protection gorgeous leaf nutrients - Google Patents
Preparation method and application of gorgeous leaf juice or gorgeous leaf powder with complete and full-protection gorgeous leaf nutrients Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/31—Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a method for preparing a gorgeous leaf juice or a gorgeous leaf powder with complete and full-bright-leaf nutrients and an application thereof, wherein the method for preparing the gorgeous leaf juice with complete and full-bright-leaf nutrients comprises the following steps: (1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice; (2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 2-5: 3000-6000 mixing; placing the mixed liquid in a water bath at 50-70 ℃ for 35-50 min to obtain a mixed liquid; (3) and centrifuging the mixed solution, and concentrating the centrifugate until the sugar degree is 8-15 to obtain the gorgeous leaf juice. The gorgeous leaf powder is obtained by pre-freezing, freeze-drying, sublimation-drying and analysis-drying gorgeous leaf juice, and can effectively protect the nutritional components and the colors of the gorgeous leaves.
Description
Technical Field
The invention relates to the technical field of food, in particular to a preparation method and application of gorgeous leaf juice or gorgeous leaf powder which is complete and full-bright-leaf nutrient substances.
Background
The gorgeous leaves are also called as variegated leaves, which are native to Marugu Islands in Indonesia and spread to Singapore, Malaysia, Srilanca, India, China, Thailand and other countries. At present, fresh leaves are produced in the world year by over 400 ten thousand tons, wherein southeast Asia accounts for more than 80 percent, and the products sold in the market mainly comprise raw materials and intermediate products such as the fresh leaves, tinctures, cattleya powder and the like, and end products such as cakes, ice creams, beverages and the like. Hainan is a planting origin and a dominant production area of leaf of variegated orchid in China, and planting areas mainly comprise Wanning, Qinhai, delirium, tombs, pavilions, guam and the like, wherein the Wanning is used as a main distribution area and a traditional utilization area, and the planting area is about 5000 mu. At present, the southern China has abundant use culture of the leaf of the cymbidium sinense, mostly takes the culture of the southern China as a carrier and a folk comprehensive use mode as a main part, and is widely applied to industries such as special catering, ornamental gardening, leisure travel and the like.
With the continuous improvement of living standard of people, the incidence rate of hyperlipemia is in a trend of obvious rising, and hyperlipemia is an important factor for inducing coronary atherosclerotic heart disease (coronary heart disease), arteriosclerosis, fatty liver, diabetes, obesity and the like. Therefore, the method has important practical significance for searching efficient and nontoxic lipid-lowering drugs.
Disclosure of Invention
The invention provides a preparation method and application of gorgeous leaf juice or gorgeous leaf powder which can completely protect full gorgeous leaf nutrients, wherein the gorgeous leaf powder can obviously reduce blood fat and has high efficiency and no toxic and side effects.
The invention adopts the following technical scheme for solving the technical problems:
a method for preparing a gorgeous leaf juice with complete and full-protection gorgeous leaf nutrients comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 2-5: 3000-6000 mixing; placing the mixed liquid in a water bath at 50-70 ℃ for 35-50 min to obtain a mixed liquid;
(3) and centrifuging the mixed solution, and concentrating the centrifugate until the sugar degree is 8-15 to obtain the gorgeous leaf juice.
Preferably, the disrupted material for cell disruption is prepared from cellulase, pectinase and CTAB at a weight ratio of 1: 0.5-2: 0.1 to 0.3.
Preferably, the disrupted material for cell disruption is a mixture of cellulase, pectinase and CTAB at a weight ratio of 1: 1.8: 0.2.
As a preferable mode, the juice extract, the crushed material for cell disruption, and the deionized water are mixed in a weight ratio of 1000: 4: 5000 mixing.
The invention also provides a preparation method of the gorgeous leaf powder with complete and full-bright-leaf nutrients, which comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 2-5: 3000-6000 mixing; placing the mixed liquid in a water bath at 50-70 ℃ for 35-50 min to obtain a mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifuged solution until the sugar degree is 8-15 to obtain multicolored leaf juice;
(4) placing the multicolored leaf juice into a freeze-drying box, and pre-freezing for 1-4 h at-50 to-40 ℃;
(5) after pre-freezing, starting a vacuum pump, and freeze-drying for 60-100 min at a vacuum degree of 18-22 Pa and a temperature of-25 to-10 ℃;
(6) after freeze drying, carrying out sublimation drying for 80-120 min at a vacuum degree of 12-16 Pa and a temperature of-5 ℃;
(7) after sublimation drying, carrying out resolution drying for 2-4 h at the vacuum degree of 5-10 Pa and the temperature of 8-15 ℃ to obtain the multicolored leaf powder.
Preferably, the disrupted material for cell disruption is prepared from cellulase, pectinase and CTAB at a weight ratio of 1: 0.5-2: 0.1 to 0.3.
Preferably, the disrupted material for cell disruption is prepared from cellulase, pectinase and CTAB at a weight ratio of 1: 1.8: 0.2.
As a preferable mode, the juice extract, the crushed material for cell disruption, and the deionized water are mixed in a weight ratio of 1000: 4: 5000 mixing.
As a preferred scheme, the step (4) is pre-frozen for 2h at-48 ℃.
The invention also provides application of the gorgeous leaf juice or the gorgeous leaf powder which is complete and full-bright-leaf nutrient substances in preparing blood fat reducing medicines.
The invention has the beneficial effects that: (1) the gorgeous leaf juice is obtained by processing broken substances for cell breakage, centrifuging and concentrating, can effectively retain nutrients in the gorgeous leaves, can fully break by selecting cellulase, pectinase and CTAB as the broken substances for cell breakage, and can better extract components in the gorgeous leaves; (2) the gorgeous leaf powder is obtained by prefreezing, freeze-drying, sublimation-drying and analysis-drying gorgeous leaf juice, and can effectively protect the nutrient components and the color (green) of the gorgeous leaves.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for preparing a gorgeous leaf juice with complete and full-protection gorgeous leaf nutrients comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 1000: 4: 5000 mixing; placing the mixed liquid in water bath at 65 deg.C for 40min to obtain mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifugate to sugar degree of 12 to obtain gorgeous leaf juice.
In this example, the disrupted material for cell disruption was prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 1.8: 0.2.
Example 2
A method for preparing a gorgeous leaf juice with complete and full-protection gorgeous leaf nutrients comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 2: 3000 mixing; placing the mixed liquid in water bath at 65 deg.C for 40min to obtain mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifugate to sugar degree of 10 to obtain gorgeous leaf juice.
In this example, the disrupted material for cell disruption was prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 0.5: 0.3.
Example 3
A method for preparing a gorgeous leaf juice with complete and full-protection gorgeous leaf nutrients comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 5: 6000 mixing; placing the mixed liquid in water bath at 65 deg.C for 40min to obtain mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifugate to sugar degree of 8 to obtain multicolored leaf juice.
In this example, the disrupted material for cell disruption was prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 2: 0.1.
Example 4
The gorgeous leaf powder described in example 4 was obtained by further performing prefreezing, freeze-drying, sublimation-drying and resolution-drying on the basis of the gorgeous leaf juice prepared in example 1.
A method for preparing a gorgeous leaf powder with complete and full-protection gorgeous leaf nutrients comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 1000: 4: 5000 mixing; placing the mixed liquid in water bath at 65 deg.C for 40min to obtain mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifugate to sugar degree of 12 to obtain gorgeous leaf juice;
(4) placing the multicolored leaf juice in a freeze-drying box, and pre-freezing at-48 deg.C for 2 hr;
(5) after pre-freezing, starting a vacuum pump, and freeze-drying for 90min at a vacuum degree of 20Pa and a temperature of-18 ℃;
(6) freeze drying, and sublimation drying at 0 deg.C and vacuum degree of 15Pa for 100 min;
(7) sublimating and drying, and performing resolution drying at 10 deg.C under vacuum degree of 8Pa for 3 hr to obtain gorgeous leaf powder.
In this example, the disrupted material for cell disruption was prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 1.8: 0.2.
Example 5
The gorgeous leaf powder described in example 5 was obtained by further performing prefreezing, freeze-drying, sublimation-drying and resolution-drying on the basis of the gorgeous leaf juice prepared in example 2.
A method for preparing a gorgeous leaf powder with complete and full-protection gorgeous leaf nutrients comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 2: 3000 mixing; placing the mixed liquid in water bath at 65 deg.C for 40min to obtain mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifugate to sugar degree of 10 to obtain gorgeous leaf juice;
(4) placing the multicolored leaf juice in a freeze-drying box, and pre-freezing at-48 deg.C for 2 hr;
(5) after pre-freezing, starting a vacuum pump, and freeze-drying for 90min at a vacuum degree of 20Pa and a temperature of-18 ℃;
(6) freeze drying, and sublimation drying at 0 deg.C and vacuum degree of 15Pa for 100 min;
(7) sublimating and drying, and performing resolution drying at 10 deg.C under vacuum degree of 8Pa for 3 hr to obtain gorgeous leaf powder.
In this example, the disrupted material for cell disruption was prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 0.5: 0.3.
Example 6
The gorgeous leaf powder described in example 6 was obtained by further performing prefreezing, freeze-drying, sublimation-drying and resolution-drying on the basis of the gorgeous leaf juice prepared in example 3.
A method for preparing a gorgeous leaf powder with complete and full-protection gorgeous leaf nutrients comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 5: 6000 mixing; placing the mixed liquid in water bath at 65 deg.C for 40min to obtain mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifugate to sugar degree of 8 to obtain multicolored leaf juice;
(4) placing the multicolored leaf juice in a freeze-drying box, and pre-freezing at-48 deg.C for 2 hr;
(5) after pre-freezing, starting a vacuum pump, and freeze-drying for 90min at a vacuum degree of 20Pa and a temperature of-18 ℃;
(6) freeze drying, and sublimation drying at 0 deg.C and vacuum degree of 15Pa for 100 min;
(7) sublimating and drying, and performing resolution drying at 10 deg.C under vacuum degree of 8Pa for 3 hr to obtain gorgeous leaf powder.
In this example, the disrupted material for cell disruption was prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 2: 0.1.
Comparative example 1
Comparative example 1 differs from example 4 in that the composition of the crushed material of the gorgeous leaf powder for cell disruption described in comparative example 1 differs from example 4, and is otherwise the same.
In this comparative example, the disrupted material for cell disruption was prepared from cellulase and pectinase at a weight ratio of 1: 1.8.
Comparative example 2
Comparative example 2 is different from example 4 in that the composition of the crushed material of the gorgeous leaf powder for cell disruption described in comparative example 2 is different from example 4, and the others are the same.
In the present comparative example, the disrupted material for cell disruption was prepared from cellulase and CTAB at a weight ratio of 1: 0.2.
Comparative example 3
Comparative example 3 differs from example 4 in that the composition of the crushed material of the gorgeous leaf powder for cell disruption described in comparative example 3 differs from example 4, and is otherwise the same.
In the present comparative example, the disrupted material for cell disruption was prepared from pectinase, CTAB at a weight ratio of 1.8: 0.2.
Comparative example 4
Comparative example 4 is different from example 4 in that the multicolored leaf powder described in comparative example 4 was obtained by drying the multicolored leaf juice described in example 1 in a constant temperature drying oven, and the others were the same.
A method for preparing a gorgeous leaf powder with complete and full-protection gorgeous leaf nutrients comprises the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 1000: 4: 5000 mixing; placing the mixed liquid in water bath at 65 deg.C for 40min to obtain mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifugate to sugar degree of 12 to obtain gorgeous leaf juice;
(4) drying the multicolored leaf juice in a constant temperature drying oven of 90 deg.C to obtain multicolored leaf powder.
To further demonstrate the effect of the present invention, the following test methods were provided:
the tested drugs are: the multicolored leaf powders of examples 4 to 6 and comparative examples 1 to 4 were each prepared with water as a solvent at a concentration of 500 mg/L.
The high-fat feed comprises the following components: the basic feed (flour 25, corn flour 20, oatmeal 25, bean cake powder 12, fish meal 8, bone meal 2, yeast powder 2, refined salt 1) 83.4%, egg yolk powder 10%, lard 5%, sodium cholate 0.5%, methimazole 0.1%, and cholesterol 1%.
Animal grouping: 90 mice were selected and randomly divided into 9 groups, a blank control group, a model control group, the gorgeous leaf powder administration groups described in examples 4 to 6 and comparative examples 1 to 4.
Modeling the hyperlipemia mouse: the basal feed is fed adaptively for 1 week, free foraging and drinking water are fed during adaptation period, the basal feed is fed to a blank control group, the other 8 groups are fed with high fat feed for 2 weeks, and then the blood is collected from the eye orbit of the mouse, and the content of Total Cholesterol (TC) in serum is measured.
The administration route is as follows: the oral administration is intragastric.
Administration mode and dose: the extract of the invention is administered at 500 mg/kg, and the blank group and the model control group are infused with equal volume of physiological saline.
The experimental period is 7 days, the administration is timed every day (eight am, 12 am and five pm), the mice are fasted for 12 h before the last administration, the eyeballs of the mice are picked up for blood collection 2h after the last administration, the blood is centrifuged for 10 min at 3500 r/min after blood coagulation, the serum TC content is separated, and the specific results are shown in table 1.
TABLE 1 test results
As can be seen from Table 1, the gorgeous leaf powder of the present invention has a good blood lipid-lowering effect.
As can be seen from comparison of examples 4-6, different preparation parameters can affect the blood lipid reducing effect of the gorgeous leaf powder, wherein example 4 is the best preparation parameter and has the best blood lipid reducing effect.
As can be seen from comparative example 4 and comparative examples 1 to 3, the blood lipid-lowering effect of the gorgeous leaf powder can be significantly affected by the composition of different cell disruptants.
As is clear from comparative example 4 and comparative example 4, the blood lipid-lowering effect of the gorgeous leaf powder can be improved by prefreezing, freeze-drying, sublimation-drying, and resolution-drying as compared with other drying methods.
In light of the foregoing description of preferred embodiments according to the invention, it is clear that many changes and modifications can be made by the person skilled in the art without departing from the scope of the invention. The technical scope of the present invention is not limited to the contents of the specification, and must be determined according to the scope of the claims.
Claims (10)
1. A method for preparing a gorgeous leaf juice for completely protecting gorgeous leaf nutrients, comprising the steps of:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 2-5: 3000-6000 mixing; placing the mixed liquid in a water bath at 50-70 ℃ for 35-50 min to obtain a mixed liquid;
(3) and centrifuging the mixed solution, and concentrating the centrifugate until the sugar degree is 8-15 to obtain the gorgeous leaf juice.
2. The method of preparing a juice of gorgeous leaves with complete and full-color nutrients as claimed in claim 1, wherein the crushed material for cell disruption is prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 0.5-2: 0.1 to 0.3.
3. The method of preparing a juice of gorgeous leaves with complete and full-color nutrients as claimed in claim 1, wherein the crushed material for cell disruption is prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 1.8: 0.2.
4. The method of preparing a juice of gorgeous leaves with complete and full-color nutrients as claimed in claim 1, wherein the juice extract, the crushed matter for cell disruption, and the deionization are mixed in a weight ratio of 1000: 4: 5000 mixing.
5. A method for preparing a gorgeous leaf powder with complete and full-protection gorgeous leaf nutrients, which is characterized by comprising the following steps:
(1) cleaning fresh multicolored leaves, drying, chopping and juicing after drying to obtain juice;
(2) mixing the juice, crushed material for cell disruption, and deionized water at a weight ratio of 1000: 2-5: 3000-6000 mixing; placing the mixed liquid in a water bath at 50-70 ℃ for 35-50 min to obtain a mixed liquid;
(3) centrifuging the mixed solution, and concentrating the centrifuged solution until the sugar degree is 8-15 to obtain multicolored leaf juice;
(4) placing the multicolored leaf juice into a freeze-drying box, and pre-freezing for 1-4 h at-50 to-40 ℃;
(5) after pre-freezing, starting a vacuum pump, and freeze-drying for 60-100 min at a vacuum degree of 18-22 Pa and a temperature of-25 to-10 ℃;
(6) after freeze drying, carrying out sublimation drying for 80-120 min at a vacuum degree of 12-16 Pa and a temperature of-5 ℃;
(7) after sublimation drying, carrying out resolution drying for 2-4 h at the vacuum degree of 5-10 Pa and the temperature of 8-15 ℃ to obtain the multicolored leaf powder.
6. The method for preparing a powder of multicolored leaves with complete nutrients of colorful leaves as claimed in claim 5, wherein the crushed material for cell disruption is prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 0.5-2: 0.1 to 0.3.
7. The method for preparing a powder of multicolored leaves with complete nutrients of colorful leaves as claimed in claim 5, wherein the crushed material for cell disruption is prepared from cellulase, pectinase, CTAB at a weight ratio of 1: 1.8: 0.2.
8. The method of preparing a powder of multicolored leaves with complete and wide variety of nutrients as claimed in claim 5, wherein said squeezed juice, crushed material for cell disruption, deionized water are mixed in a weight ratio of 1000: 4: 5000 mixing.
9. The method for preparing a multi-colored leaf powder with complete multi-colored leaf nutrients as claimed in claim 5, wherein the step (4) is pre-frozen at-48 ℃ for 2 hours.
10. Use of the diversity juice or powder of complete and full-color leaf nutrients according to any one of claims 1 to 9 in the preparation of a blood lipid lowering medicament.
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| CN113368970A (en) * | 2021-06-11 | 2021-09-10 | 中国热带农业科学院 | Preparation method of multicolored leaf powder |
| CN113399025A (en) * | 2021-06-11 | 2021-09-17 | 中国热带农业科学院 | Food processing system for completely protecting colorful leaf nutrients |
| CN113397153A (en) * | 2021-07-05 | 2021-09-17 | 龚政宇 | A multicolored food and its preparation method |
| CN114403435A (en) * | 2022-01-25 | 2022-04-29 | 中国热带农业科学院橡胶研究所 | Preparation method and application of gorgeous powder |
| CN115119747A (en) * | 2022-04-30 | 2022-09-30 | 中国热带农业科学院海口实验站 | Leaf tissue culture medium of cymbidium maculatum and tissue culture rapid propagation method |
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| CN113368970A (en) * | 2021-06-11 | 2021-09-10 | 中国热带农业科学院 | Preparation method of multicolored leaf powder |
| CN113399025A (en) * | 2021-06-11 | 2021-09-17 | 中国热带农业科学院 | Food processing system for completely protecting colorful leaf nutrients |
| CN113399025B (en) * | 2021-06-11 | 2022-08-23 | 中国热带农业科学院 | Food processing method for complete and full-protection colorful leaf nutrient substances |
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| CN114403435A (en) * | 2022-01-25 | 2022-04-29 | 中国热带农业科学院橡胶研究所 | Preparation method and application of gorgeous powder |
| CN114403435B (en) * | 2022-01-25 | 2023-09-19 | 中国热带农业科学院橡胶研究所 | Preparation method and application of colorful powder |
| CN115119747A (en) * | 2022-04-30 | 2022-09-30 | 中国热带农业科学院海口实验站 | Leaf tissue culture medium of cymbidium maculatum and tissue culture rapid propagation method |
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