CN112501001A

CN112501001A - Microorganism detection equipment and detection method

Info

Publication number: CN112501001A
Application number: CN202011305097.9A
Authority: CN
Inventors: 王青兰
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-19
Filing date: 2020-11-19
Publication date: 2021-03-16

Abstract

The invention relates to the technical field of microorganism detection, and discloses a microorganism detection device and a detection method, wherein the microorganism detection device comprises a bottom barrel, the bottom barrel is of a hollow cylindrical structure, the top of the bottom barrel is open, a main motor is fixedly connected with the center of the inner bottom surface of the bottom barrel, a barrel cover is taken down from a support, a test tube is placed in a fixed barrel, a support column is sleeved on a limit hole, a main spring is popped out to pop a clamping column to the inner side wall of the limit hole, the support column is fixedly sleeved with the limit hole, a slave motor is started, a slave motor rotates to drive a steering wheel to rotate, a rotating shaft is further driven to rotate, the rotating shaft does circular motion to drive a guide shaft to do piston motion to further drive the clamping column to do piston motion to drive a sliding block to move back and forth on a sliding rod, and finally, sample liquid in the test tube is uniformly oscillated, and the sample liquid in the test tube can be sufficiently oscillated and uniformly mixed, the influence of the uneven oscillation on the detection is avoided.

Description

Microorganism detection equipment and detection method

Technical Field

The invention relates to the technical field of microbial detection, in particular to a microbial detection device and a microbial detection method.

Background

Microorganisms are low organisms which are widely present in nature and have tiny, numerous and invisible forms, and can be observed only by magnifying the organisms by hundreds of times or thousands of times by means of an optical microscope or an electronic microscope, and the microorganisms have various types and can be divided into three categories according to the sizes, structures, compositions and the like: the non-cell type microorganism is the lowest organisms which have the smallest physique, are measured in nanometers, have the simplest structure, only contain one nucleic acid or only infectious protein particles, have super parasitism, can replicate only in living susceptible cells and are easy to mutate.

The existing microorganism detection is generally directly operated and detected manually, sufficient oscillation and uniform mixing are difficult to achieve when sample liquid oscillates, great influence is easily caused during detection, pure and impurity-free sample liquid is difficult to obtain when the sample liquid is extracted, errors can be caused to detection by more impurities, the content of microorganisms in the extracted sample liquid is small and difficult to detect, the output of the sample liquid is difficult to control, and the sample liquid is easy to spill and waste.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a microorganism detection device, which has the advantages of uniform sample liquid oscillation mixing, easy extraction of pure sample liquid and the like, and solves the problems that the sample liquid is difficult to be sufficiently and uniformly oscillated during oscillation, great influence is easily caused during detection, pure and impurity-free sample liquid is difficult to obtain during sample liquid extraction, more impurities may cause errors to detection, the content of microorganisms in the extracted sample liquid is low, the output of the sample liquid is difficult to control, and the sample liquid is easy to spill and waste.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a microorganism detection device comprises a bottom barrel, wherein the bottom barrel is of a hollow cylindrical structure, the top of the bottom barrel is open, a main motor is fixedly connected to the center of the inner bottom surface of the bottom barrel, the main motor is of an existing structure and is not described herein any more, the main motor is a Y80M2-2 direct current motor, a rotating disk is arranged above the main motor, the rotating disk is of a cylindrical structure, a supporting hole is formed in the center of the top surface of the rotating disk, the supporting hole is a circular through hole, the inner side wall of the supporting hole is fixedly connected with the outer side wall of a shaft of the main motor, four stirring rods are fixedly connected to the top surface of the rotating disk, the stirring rods are of a cylindrical structure and are located on a contour line of the same circle, fixing columns are fixedly connected to two sides of the top of the inner side wall of the bottom barrel respectively, the fixing columns are of a cylindrical structure, the bottom of the bottom barrel cover is open, the bottoms of the outer side walls of two sides of the bottom barrel cover are respectively provided with a curved groove, the curved grooves are T-shaped grooves, the middle part of the outer side wall of the bottom barrel is provided with a limit hole, the inner side wall of the limit hole is fixedly connected with a main sample outlet pipe, the main sample outlet pipe is of a hollow cylindrical structure, the bottom surface and the bottom surface of the main sample outlet pipe are both open, a connecting hole is formed in one side of the outer side wall of the sample outlet pipe and is a circular hole in a sliding and sleeved mode, the main sample outlet pipe is of a hollow cylindrical structure, the bottom surface and the bottom surface of the main sample outlet pipe are both open, the outer side wall of the main sample outlet pipe is attached together, the connecting hole is a circular hole, the inner part of the connecting hole is fixedly connected with a secondary spring, the secondary spring is of an existing, the filter is cylindrical structure, a plurality of filtration pores have been seted up on the filter, the filtration pore is circular through-hole, the through-hole has been seted up from the lateral wall opposite side that goes out the appearance pipe, the inside wall sliding connection of through-hole has the steady axle, the one side of steady axle links together with the bottom from going out the appearance pipe always, the bottom surface fixedly connected with incubator of end bucket, the incubator is current structure, do not redundantly here, the end bucket is located one side top surface of incubator, the opposite side top surface fixedly connected with vibration box of incubator, the vibration box is cavity rectangle structure, the top surface of vibration box is uncovered, two slide bars of both sides inside wall middle part fixedly connected with of vibration box, the slide bar is cylindrical structure, be provided with the slider on the slide bar, the slider is the rectangle structure, two connecting holes have all been seted up to the both sides of slider.

Preferably, a clamping groove is formed in the position five centimeters below the middle of the inner side wall of the bottom barrel, the clamping groove is an arc-shaped groove, ball clamping grooves are formed in the top and the bottom of the clamping groove and arc-shaped grooves, ball clamping grooves are formed in the top surface and the bottom surface of the rotating disk, and the four ball clamping grooves are on the same vertical line.

Preferably, a plurality of sliding balls are arranged between the top surface ball clamping grooves of the rotating disc and the top surface ball clamping grooves of the clamping grooves, the sliding balls are of a spherical structure, a plurality of sliding balls are arranged between the bottom surface ball clamping grooves of the rotating disc and the bottom surface ball clamping grooves of the clamping grooves, and the sliding balls are movably clamped with the ball clamping grooves.

Preferably, the stabilizing shaft is fixedly connected with a water blocking plate which is of a cylindrical structure, and the outer side wall of the water blocking plate is attached to the inner side wall of the main sample outlet pipe.

Preferably, the top surface of the sliding block is fixedly connected with three fixed barrels, the fixed barrels are of hollow cylindrical structures, and the top surfaces of the fixed barrels are open.

Preferably, the top surface both sides of fixed bucket fixedly connected with respectively support, and the support column is cylindrical structure, the trompil has been seted up to the top surface lateral wall that supports and live, and the trompil is the circular port, and the inside fixedly connected with main spring of trompil, main spring are current structure, do not do the perplexing here, and one side fixedly connected with joint post of main spring, joint post are cylindrical structure.

Preferably, the top surface of the fixed barrel is provided with a barrel cover, the barrel cover is of a cylindrical structure, and the bottom surface of the barrel cover is provided with two limiting holes, so that the supporting column can be fixedly sleeved on the limiting holes.

Preferably, one side middle part fixedly connected with card post of slider, card post are cylindrical structure, one side of card post is seted up flutedly, and the recess is the rectangular channel, both sides inside wall middle part fixedly connected with spliced pole of recess, the spliced pole is cylindrical structure.

Preferably, a guide shaft rod is arranged on the connecting column, the guide shaft rod is of a rectangular structure, a stable hole is formed in one side of the guide shaft rod and is a circular through hole, the inner side wall of the stable hole is in sliding sleeve joint with the outer side wall of the connecting column, a mounting hole is formed in the other side of the guide shaft rod and is a circular through hole, a rotating shaft is sleeved on the inner side wall of the mounting hole, the side view of the rotating shaft is of a T-shaped structure, a steering wheel is fixedly connected to one side of the rotating shaft and is of a cylindrical structure, the rotating shaft is located on the edge of one side of the steering wheel, a slave motor is fixedly connected to the circle center of the other side of the steering wheel, the slave motor is of an existing structure, repeated description is omitted, the slave motor is a Y100L-2 alternating current motor, and the bottom.

In order to overcome the defects of the prior art, the invention also provides a microorganism detection method, which comprises the following specific steps:

s1: taking out the food, pouring the food from the open top of the bottom barrel and adding water until the water overflows the food;

s2: starting a main motor, wherein the main motor rotates to drive a rotating disc to rotate so as to drive a stirring rod to rotate, so that food is stirred by the stirring rod and fully fused with water, and sample liquid is taken out from a main sample outlet pipe;

s3: placing 10mL of sample solution from the main sample outlet pipe into a test tube containing 90mL of sterilized normal saline, and making uniform dilution of 1:10 after sufficient oscillation of a sliding block;

s4: sucking 1:10 of 1mL of the diluent by using a 1mL sterilizing suction tube, injecting the 1:10 of the diluent into a test tube containing 9mL of sterilized normal saline, and fully oscillating and uniformly mixing the diluent by using a slide block to prepare a 1:100 diluent;

s5: taking another 1mL of sterilized straws, sequentially making 10 times of gradually increased diluents according to the operation, changing 1mL of sterilized straws for each gradually increased dilution, and inoculating 3 tubes for each dilution;

s6: inoculating the sample liquid into lactose and bile salt fermentation test tubes, wherein the inoculation amount is more than 1mL, using double-material lactose bile salt fermentation test tubes and less than 1mL, using single-material lactose bile salt fermentation tubes, inoculating 3 tubes at each dilution degree, placing in an incubator 5 at about 36 ℃, and culturing for 24h, wherein if all the lactose bile salt fermentation test tubes do not produce gas, the obtained coliform group is negative.

(III) advantageous effects

Compared with the prior art, the invention provides a microorganism detection device, which has the following beneficial effects:

1. the microbial detection equipment comprises a barrel cover, a test tube is placed in a fixed barrel by taking down the barrel cover from a support, a support column is sleeved on a limit hole, a main spring is popped out to pop a clamping column to the inner side wall of the limit hole, the support column is fixedly sleeved with the limit hole, a slave motor is started, the slave motor rotates to drive a steering wheel to rotate and further drive a rotating shaft to rotate, the rotating shaft does circular motion to drive a guide shaft rod to do piston motion and further drive the clamping column to do piston motion, the clamping column does piston motion to drive a sliding block to do reciprocating motion on a sliding rod, finally, sample liquid in the test tube is uniformly oscillated, the sample liquid is fully oscillated by the sliding block to be made into 1:10 uniform diluent, 1mL 1:10 diluent is sucked by a 1mL sterilization suction tube and is injected into the test tube containing 9mL sterilization physiological saline, the piston motion is repeated, the sample liquid is fully oscillated and uniformly mixed by the sliding block, the dilution is made to be 1:100, so that the sample liquid in the test tube can be fully and uniformly mixed in an oscillating way, and the influence of nonuniform oscillation on detection is avoided.

2. This a microbiological inspection check out test set, through taking out food, pour into and add water with food from the uncovered department in top of end bucket, until water does not pass the food, start main motor, main motor rotates and drives the rotary disk and rotate, and then drive the stirring rod and rotate, the stirring rod rotates and makes food be stirred up, thereby can with water intensive mixing, a period of time later, the rotation and stabilization axle, make the board that blocks water be the right angle state with preceding, the appearance liquid after the mixture filters through the filtration pore, obtain the appearance liquid that does not have impurity, and then reach in the test tube of placing in advance through main appearance pipe, thereby make the purer of the appearance liquid that obtains not have impurity, the influence of too much food impurity to the detection has been avoided.

3. This microbial detection check out test set through four stirring rods that set up on the rotary disk, can make the food that gets into the end bucket fully in the end bucket by the misce bene to can make the microorganism in the food get into the aquatic after adding water, fully fuse with water, thereby contain a large amount of microorganisms in the messenger's extract, detection after convenient.

4. This microbial detection check out test set offers the arc draw-in groove through five centimetres departments in bottom bucket inside wall middle part below to the card ball groove has been offered at the top surface of arc draw-in groove and rotary disk and bottom surface edge, places a plurality of slide balls on the card ball groove, and the rotary disk that makes can be quick rotation on the inside wall of bottom bucket, has avoided the friction of rotary disk and draw-in groove to cause the damage of equipment.

5. This microbial detection check out test set sets up water-blocking plate and stable axle through inside wall one side at main appearance pipe for the appearance liquid output that flows out in the bucket of end can be controlled, has avoided output too much to cause unrestrained and the waste of appearance liquid.

6. This microbial detection check out test set cup joints from going out the appearance pipe through the inside activity that goes out the appearance pipe at owner to set up from spring and bullet post on going out the appearance pipe, make principal and subordinate go out the appearance pipe and can freely stretch out and draw back, improved the usability of equipment.

7. This microorganism inspection check out test set through set up the end bung on the end bucket to set up curved groove, make end bung can be in the same place with the rotatory joint of fixed column, avoided main motor operation process to produce vibrations and caused to draw the sample liquid unrestrained.

Drawings

FIG. 1 is a schematic view of an overall split three-dimensional structure according to the present invention;

FIG. 2 is a schematic perspective view of the bottom barrel of the present invention;

FIG. 3 is a schematic perspective view of the vibration box of the present invention;

FIG. 4 is a schematic side view of the bottom barrel of the present invention;

FIG. 5 is a schematic perspective view of a clip post according to the present invention;

FIG. 6 is an enlarged partial view of A in FIG. 4 according to the present invention;

FIG. 7 is a schematic perspective view of a stationary barrel according to the present invention;

FIG. 8 is a schematic perspective view of a main sampling tube according to the present invention;

fig. 9 is a schematic view of a barrel cover inverted structure of the present invention.

In the figure: the device comprises a bottom barrel 1, a vibrating box 2, a guide shaft rod 3, a fixed barrel 4, a warm box 5, branch holes 6, a stirring rod 7, a rotating disk 8, a main motor 9, a main sample outlet pipe 10, a slide rod 11, a slide block 12, a connecting hole 13, a clamping groove 14, a secondary motor 15, a steering wheel 16, a rotating shaft 17, a mounting hole 18, a stabilizing hole 19, a clamping column 20, a groove 21, a connecting column 22, a sliding ball 23, a ball clamping groove 24, a barrel cover 25, a supporting column 26, an opening 27, a main spring 28, a clamping column 29, a through hole 30, a filter plate 31, a water blocking plate 32, a stabilizing shaft 33, a filter hole 34, a filter hole 35, a secondary sample outlet pipe 36, a secondary spring 37 elastic column 38, a connecting hole 39 fixing column 39, a bent groove 40 and.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As described in the background, the prior art has shortcomings, and in order to solve the above technical problems, the present application proposes a microbiological test device:

example 1

In a typical embodiment of the present application, as shown in fig. 1 to 9, a microorganism testing and detecting apparatus includes a bottom barrel 1, the bottom barrel 1 is a hollow cylindrical structure, the top of the bottom barrel 1 is open, a main motor 9 is fixedly connected to the center of the inner bottom surface of the bottom barrel 1, the main motor 9 is an existing structure, details are not described herein, the main motor 9 is a Y80M2-2 dc motor, a rotating disk 8 is arranged above the main motor 9, the rotating disk 8 is a cylindrical structure, a branch hole 6 is formed in the center of the top surface of the rotating disk 8, the branch hole 6 is a circular through hole, the inner side wall of the branch hole 6 is fixedly connected to the outer side wall of the shaft of the main motor 9, four stirring rods 7 are fixedly connected to the top surface of the rotating disk 8, the stirring rods 7 are cylindrical structures, the stirring rods 7 are all located on the contour line of the same circle, fixed columns 39 are respectively fixedly connected to both sides of the inner side wall, the fixing column 39 is a cylindrical structure, a bottom barrel cover 41 is arranged above the bottom barrel 1, the bottom barrel cover 41 is a hollow cylindrical structure, the bottom of the bottom barrel cover 41 is open, the bottom of the outer side walls of the two sides of the bottom barrel cover 41 are respectively provided with a curved groove 40, the curved grooves 40 are T-shaped grooves, the middle part of the outer side wall of the bottom barrel 1 is provided with a limiting hole, the inner side wall of the limiting hole is fixedly connected with a main sample outlet pipe 10, the main sample outlet pipe 10 is a hollow cylindrical structure, the bottom surface and the bottom surface of the main sample outlet pipe 10 are both open, the inner side wall of the main sample outlet pipe 10 is slidably sleeved with a secondary sample outlet pipe 35, the secondary sample outlet pipe 35 is a hollow cylindrical structure, the bottom surface and the bottom surface of the secondary sample outlet pipe 35 are both open, the outer side wall of the secondary sample outlet pipe 35 is attached to the inner side wall of the main sample outlet pipe 10, one side of the outer side wall of the sample, the spring 36 is of a conventional structure, which is not described herein in detail, an elastic column 37 is fixedly connected to one side of the spring 36, the elastic column 37 is of a cylindrical structure, a filter plate 31 is fixedly connected to one side of the inner side wall of a sample outlet pipe 35, the filter plate 31 is of a cylindrical structure, a plurality of filter holes 34 are formed in the filter plate 31, the filter holes 34 are circular through holes, through holes 30 are formed in the other side of the outer side wall of the sample outlet pipe 35, a stabilizing shaft 33 is slidably connected to the inner side wall of the through holes 30, one side of the stabilizing shaft 33 is always connected to the bottom of the sample outlet pipe 35, an incubator 5 is fixedly connected to the bottom surface of the bottom barrel 1, the incubator 5 is of a conventional structure, which is not described herein in detail, the bottom barrel 1 is located on the top surface of one side of the incubator 5, a vibration box 2 is fixedly connected to the top surface of the other side of the incubator 5, the vibration box 2, the slide bar 11 is a cylindrical structure, a slide block 12 is arranged on the slide bar 11, the slide block 12 is a rectangular structure, two connecting holes 13 are formed in two sides of the slide block 12, the connecting holes 13 are circular through holes, and the connecting holes 13 in the two sides correspond to one another.

Further, in the above scheme, a clamping groove 14 is formed in the position five centimeters below the middle portion of the inner side wall of the bottom barrel 1, the clamping groove 14 is an arc-shaped groove, ball clamping grooves 24 are formed in the top and the bottom of the clamping groove 14, the ball clamping grooves 24 are arc-shaped grooves, the ball clamping grooves 24 are formed in the top surface and the bottom surface of the rotating disk 8, and the four ball clamping grooves 24 are all on the same vertical line, so that the rotating disk 8 can be movably clamped in the bottom barrel 1, and the rotating disk 8 is prevented from moving when the main motor 9 rotates.

Further, in the above scheme, a plurality of sliding balls 23 are arranged between the top surface ball-clamping groove 24 of the rotating disk 8 and the top surface ball-clamping groove 24 of the clamping groove 14, the sliding balls 23 are in a spherical structure, a plurality of sliding balls 23 are arranged between the bottom surface ball-clamping groove 24 of the rotating disk 8 and the bottom surface ball-clamping groove 24 of the clamping groove 14, and the sliding balls 23 and the ball-clamping grooves 24 are movably clamped together, so that the rotating disk 8 and the clamping groove 14 can be movably clamped together.

Further, in the above solution, the stabilizing shaft 33 is fixedly connected with a water blocking plate 32, the water blocking plate 32 is a cylindrical structure, and an outer side wall of the water blocking plate 32 is attached to an inner side wall of the main sample outlet pipe 10, so that liquid outflow can be controlled, and liquid leakage is avoided.

Further, in above-mentioned scheme, the three fixed bucket 4 of top surface fixedly connected with of slider 12, fixed bucket 4 is hollow cylinder structure, and the top surface of fixed bucket 4 is uncovered to the messenger places the test tube of liquid and can fix, causes empting of test tube when having avoided slider 12 to vibrate.

Further, in the above scheme, the two sides of the top surface of the fixed barrel 4 are respectively and fixedly connected with a support 26, the support column 26 is of a cylindrical structure, the outer side wall of the top surface of the support 26 is provided with an opening 27, the opening 27 is a circular hole, the inside of the opening 27 is fixedly connected with a main spring 28, the main spring 28 is of an existing structure, details are not repeated herein, one side of the main spring 28 is fixedly connected with a clamping column 29, and the clamping column 29 is of a cylindrical structure, so that the fixed barrel 4 can be fixedly connected with the barrel cover 25.

Further, in the above scheme, a barrel cover 25 is arranged on the top surface of the fixed barrel 4, the barrel cover 25 is of a cylindrical structure, and two limiting holes are formed in the bottom surface of the barrel cover 25, so that the supporting column 26 can be fixedly sleeved on the limiting holes.

Further, in the above scheme, one side middle part of the slider 12 is fixedly connected with the clamping column 20, the clamping column 20 is of a cylindrical structure, one side of the clamping column 20 is provided with the groove 21, the groove 21 is a rectangular groove, the middle parts of the inner side walls of the two sides of the groove 21 are fixedly connected with the connecting columns 22, and the connecting columns 22 are of a cylindrical structure, so that the guide shaft rod 3 can be movably connected to the clamping column 20.

Further, in the above scheme, the connecting column 22 is provided with the guide shaft rod 3, the guide shaft rod 3 is of a rectangular structure, one side of the guide shaft rod 3 is provided with a stabilizing hole 19, the stabilizing hole 19 is a circular through hole, an inner side wall of the stabilizing hole 19 is slidably sleeved with an outer side wall of the connecting column 22, the other side of the guide shaft rod 3 is provided with a mounting hole 18, the mounting hole 18 is a circular through hole, an inner side wall of the mounting hole 18 is sleeved with a rotating shaft 17, a side view of the rotating shaft 17 is of a T-shaped structure, one side of the rotating shaft 17 is fixedly connected with a steering wheel 16, the steering wheel 16 is of a cylindrical structure, the rotating shaft 17 is located on one side edge of the steering wheel 16, a center of the other side of the steering wheel 16 is fixedly connected with the slave motor 15, the slave motor 15 is of an existing structure, the slave motor 15 is an ac motor Y100L-2, a bottom surface of the slave motor 15 is, so that the slider 12 can perform a piston motion to fully oscillate the liquid.

Example 2

s1: taking out the food, pouring the food from the open top of the bottom barrel 1 and adding water until the water overflows the food;

s2: starting a main motor 9, wherein the main motor 9 rotates to drive a rotating disc 8 to rotate so as to drive a stirring rod 7 to rotate, so that food is stirred by the stirring rod 7 and is fully fused with water, and sample liquid is taken out from a main sample outlet pipe 10;

When the device is used, food is taken out, the food is poured into the bottom barrel 1 from the open top and added with water until the water is over the food, the main motor 9 is started, the main motor 9 rotates to drive the rotating disc 8 to rotate, the stirring rod 7 is further driven to rotate, the stirring rod 7 rotates to enable the food to be smashed, so that the food can be fully mixed with the water, after a period of time, the stabilizing shaft 33 is rotated to enable the water blocking plate 32 to be in a right-angle state with the front, the mixed sample liquid is filtered through the filtering hole 34 to obtain the sample liquid without impurities, the sample liquid reaches a test tube placed in advance through the main sample outlet tube 10, 10mL of the sample liquid from the main sample outlet tube 10 is placed in a test tube with 90mL of sterilized normal saline, the barrel cover 25 is taken down from the support 26, the test tube is placed in the fixed barrel 4, the support 26 is sleeved on the limiting hole, the main spring 28 is popped to pop up the clamping column 29 to be popped onto the inner side, and further fixedly sleeving the support column 26 with the limiting hole, starting the slave motor 15, driving the steering wheel 16 to rotate by rotating the slave motor 15, further driving the rotating shaft 17 to rotate, enabling the rotating shaft 17 to do circular motion to enable the guide shaft rod 3 to do piston motion, further driving the clamping column 20 to do piston motion, enabling the clamping column 20 to do piston motion to drive the sliding block 12 to move back and forth on the sliding rod 11, finally enabling the sample liquid in the test tube to be uniformly oscillated, making 1:10 uniform diluent after the sliding block 12 is fully oscillated, sucking 1:10 diluent by using a 1mL sterilization suction tube, injecting into a test tube containing 9mL sterilization physiological saline, repeating the piston motion, fully oscillating and uniformly mixing by using the sliding block 12, and making the diluent with the ratio of 1: 100.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A microbiological test detection device comprising a bottom tub (1), characterized in that: the bottom barrel is characterized in that a main motor (9) is fixedly connected to the center of the inner bottom surface of the bottom barrel (1), a rotating disk (8) is arranged above the main motor (9), branch holes (6) are formed in the circle center of the top surface of the rotating disk (8), the inner side walls of the branch holes (6) are fixedly connected with the outer side wall of a shaft of the main motor (9), a plurality of stirring rods (7) are fixedly connected to the top surface of the rotating disk (8), the stirring rods (7) are located on the contour line of the same circle, fixing columns (39) are respectively fixedly connected to two sides of the top of the inner side wall of the bottom barrel (1), a bottom barrel cover (41) is arranged above the bottom barrel (1), curved grooves (40) are respectively formed in the bottoms of the outer side walls on two sides of the bottom barrel cover (41), a limiting hole is formed in the middle of the outer side wall of the bottom barrel (1), a main sample outlet pipe (10) is fixedly connected, the outer side wall of the sample outlet pipe (35) is attached to the inner side wall of the main sample outlet pipe (10), a connecting hole (38) is formed in one side of the outer side wall of the sample outlet pipe (35), a secondary spring (36) is fixedly connected to the inside of the connecting hole (38), a spring column (37) is fixedly connected to one side of the spring (36), a filter plate (31) is fixedly connected to one side of the inner side wall of the sample outlet pipe (35), a plurality of filter holes (34) are formed in the filter plate (31), a through hole (30) is formed in the other side of the outer side wall of the sample outlet pipe (35), a stabilizing shaft (33) is connected to the inner side wall of the through hole (30) in a sliding mode, one side of the stabilizing shaft (33) is always connected with the bottom surface of the sample outlet pipe (35), a temperature box (5) is fixedly connected to the bottom surface of a bottom barrel (1), the bottom barrel (1) is located on the top surface, the middle parts of the inner side walls of two sides of the vibration box (2) are fixedly connected with a plurality of sliding rods (11), the sliding rods (11) are provided with sliding blocks (12), two sides of each sliding block (12) are provided with a plurality of connecting holes (13), and the connecting holes (13) of the two sides are in one-to-one correspondence; a clamping column (20) is fixedly connected to the middle of one side of the sliding block (12), a groove (21) is formed in one side of the clamping column (20), and connecting columns (22) are fixedly connected to the middle of the inner side walls of the two sides of the groove (21);

be provided with guide shaft pole (3) on spliced pole (22), stabilization hole (19) have been seted up to one side of guide shaft pole (3), the inside wall of stabilization hole (19) is slided to cup joint with the lateral wall of spliced pole (22) and is in the same place, and mounting hole (18) have been seted up to the opposite side of guide shaft pole (3), pivot (17) have been cup jointed to the inside wall of mounting hole (18), one side fixedly connected with steering wheel (16) of pivot (17), pivot (17) are located one side edge of steering wheel (16), fixedly connected with from motor (15) are located to the opposite side centre of a circle of steering wheel (16), from the bottom surface of motor (15) and one side inside wall fixed connection of vibration case (2) together.

2. A microbiological test detection apparatus according to claim 1 wherein: the bottom barrel is characterized in that a clamping groove (14) is formed in the position five centimeters below the middle of the inner side wall of the bottom barrel (1), ball clamping grooves (24) are formed in the top and the bottom of the clamping groove (14), the ball clamping grooves (24) are formed in the top surface and the bottom surface of the rotating disk (8), and the four ball clamping grooves (24) are all on the same vertical line.

3. A microbiological test detection apparatus according to claim 2, wherein: a plurality of sliding balls (23) are arranged between the top surface ball clamping groove (24) of the rotating disk (8) and the top surface ball clamping groove (24) of the clamping groove (14), a plurality of sliding balls (23) are arranged between the bottom surface ball clamping groove (24) of the rotating disk (8) and the bottom surface ball clamping groove (24) of the clamping groove (14), and the sliding balls (23) are movably clamped with the ball clamping grooves (24).

4. A microbiological test detection apparatus according to claim 1 wherein: the stable shaft (33) is fixedly connected with a water blocking plate (32), and the outer side wall of the water blocking plate (32) is attached to the inner side wall of the main sample outlet pipe (10).

5. A microbiological test detection apparatus according to claim 1 wherein: the top surface of the sliding block (12) is fixedly connected with three fixed barrels (4).

6. The microbiological test detection device according to claim 5, wherein: the top surface both sides of fixed bucket (4) are fixedly connected with respectively and support (26), open pore (27) have been seted up to the top surface lateral wall that supports (26), the inside fixedly connected with main spring (28) of open pore (27), one side fixedly connected with joint post (29) of main spring (28).

7. The microbiological test detection device according to claim 5, wherein: a barrel cover (25) is arranged on the top surface of the fixed barrel (4), and two limiting holes are formed in the bottom surface of the barrel cover (25).

8. A method for detecting a microorganism test, which comprises the steps of using the microorganism test detection apparatus according to any one of claims 1 to 7 for detection:

s1: taking out the food, pouring the food from the open top of the bottom barrel (1) and adding water until the water overflows the food;

s2: starting a main motor (9), wherein the main motor (9) rotates to drive a rotating disc (8) to rotate so as to drive a stirring rod (7) to rotate, so that food is stirred by the stirring rod (7) and is fully fused with water, and sample liquid is taken out from a main sample outlet pipe (10);

s3: 10mL of sampling liquid from the main sample outlet pipe (10) is placed in a test tube with 90mL of sterilized normal saline, and is made into uniform dilution of 1:10 after sufficient oscillation of a sliding block (12);

s4: sucking 1:10 of 1mL of the diluent by using a 1mL sterilizing suction tube, injecting the 1:10 of the diluent into a test tube containing 9mL of sterilized normal saline, and fully oscillating and uniformly mixing the diluent by using a slide block (12) to prepare a 1:100 diluent;