CN212864764U

CN212864764U - Microbial shaking culture device

Info

Publication number: CN212864764U
Application number: CN202021448360.5U
Authority: CN
Inventors: 黄倩; 李益兰; 程少林
Original assignee: Chongqing Three Gorges University
Current assignee: Chongqing Three Gorges University
Priority date: 2020-07-21
Filing date: 2020-07-21
Publication date: 2021-04-02
Anticipated expiration: 2030-07-21

Abstract

The utility model discloses a microbial oscillation culture device, including blake bottle and the oscillator of drive blake bottle reciprocating motion, the oscillator includes fixed platform, moving platform, guide structure and actuating mechanism, actuating mechanism includes the motor that sets up on fixed platform, the middle part is fixed on the power output shaft of motor disc, set up at the drive pin of disc limit portion and set up the driving plate in the moving platform bottom, the middle part of driving plate is provided with vertical straight flute, the drive pin is inserted in the straight flute and with straight flute sliding fit, the axial of the power output shaft of motor is perpendicular to the axial of guide bar; and a fixing mechanism for fixing the culture bottle is arranged on the top surface of the moving platform. The utility model discloses can drive blake bottle reciprocating motion to make the culture medium in the blake bottle can take place the oscillation at reciprocating motion in-process, can accelerate the oxygen in the air to dissolve to the culture medium, in order to guarantee the supply of dissolved oxygen in the culture medium, can improve aerobic microorganism's cultivation efficiency.

Description

Microbial shaking culture device

Technical Field

The utility model relates to a microbial cultivation device, in particular to microbial oscillation culture device.

Background

In the process of culturing aerobic microorganisms, the supply amount of dissolved oxygen in the culture medium needs to be ensured so as to improve the culture efficiency.

Meanwhile, in the prior art, a culture bottle for culturing the good-sample microorganisms does not have a filtering function. In the process of culturing the good-sample microorganisms, microorganisms in the liquid to be tested are generally filtered and enriched by a membrane filter device, and then the filter membrane is transferred to a culture flask for culture. The transfer filter membrane needs to be disassembled from the filter device, the operation is complex, and the transfer filter membrane is easily polluted by microorganisms in the air in the process.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model aims at providing a microbial shaking culture device to solve the supply problem of dissolved oxygen in the culture medium among the present good appearance microbial cultivation process, and solve present blake bottle and do not have filtering capability, microbial filtration enrichment and cultivation are accomplished by different devices, transfer filter membrane troublesome poeration, be polluted technical problem easily.

The utility model discloses microorganism vibration culture device, including the blake bottle, still include the oscillator of drive blake bottle reciprocating motion, the oscillator includes fixed platform, moving platform, guide structure and actuating mechanism, guide structure includes setting up the support on fixed platform, the level sets up two parallel guide bars on the support and sets up the sliding plate on two guide bars respectively, the sliding plate is with guide bar sliding fit, moving platform sets up on the slide; the driving mechanism comprises a motor arranged on the fixed platform, a disc, a transmission pin and a transmission plate, wherein the middle part of the disc is fixed on a power output shaft of the motor, the transmission pin is arranged on the edge of the disc, the transmission plate is arranged at the bottom of the movable platform, a vertical straight groove is formed in the middle part of the transmission plate, the transmission pin is inserted into the straight groove and is in sliding fit with the straight groove, and the axial direction of the power output shaft of the motor is perpendicular to the axial direction of the guide rod; and a fixing mechanism for fixing the culture bottle is arranged on the top surface of the moving platform.

Further, the fixing mechanism is an elastic clamping piece.

Further, the culture bottle comprises a bottle body, a bottle bottom, a microbial filter membrane, a supporting plate with holes, a first sealing ring and a second sealing ring, the lower end of the bottle body is connected with the upper end of the bottle bottom through threads, an annular flange is arranged on the inner wall of the lower end of the bottle body, and the first sealing ring, the microbial filter membrane, the supporting plate and the second sealing ring are sequentially arranged between the annular flange and the upper end face of the bottle bottom from top to bottom;

the side wall of the bottle bottom is provided with a pipe connector and a sealing cover arranged on the pipe connector.

The utility model has the advantages that:

1. the utility model discloses microorganism vibration culture apparatus, it can drive blake bottle reciprocating motion to make the culture medium in the blake bottle can take place the vibration at reciprocating motion in-process, can accelerate the oxygen in the air to dissolve to the culture medium, in order to guarantee the supply of dissolved oxygen in the culture medium, can improve aerobic microorganism's cultivation efficiency.

2. The utility model discloses microorganism shake culture apparatus, its blake bottle possess simultaneously filter microorganism and cultivate the function of microorganism for after filtering the enrichment microorganism through the filter membrane, can directly carry out the microorganism culture to adding liquid culture medium in the blake bottle, no longer need shift the filter membrane, simplified microorganism culture operation process greatly, improved microorganism culture work efficiency, and avoided shifting the pollution risk that the filter membrane exists.

Drawings

FIG. 1 is a schematic perspective view of a shaking culture apparatus for microorganisms;

FIG. 2 is a schematic view of the structure of a culture flask;

fig. 3 is an enlarged schematic view of the portion P in fig. 2.

Detailed Description

The invention is further described with reference to the following figures and examples.

As shown in the figure, the microorganism shaking culture device of the embodiment comprises a culture bottle 1 and an oscillator for driving the culture bottle to reciprocate, wherein the oscillator comprises a fixed platform 2, a movable platform 3, a guide structure and a driving mechanism, the guide structure comprises a support 4 arranged on the fixed platform, two guide rods 5 horizontally arranged on the support and parallel to each other, and sliding plates 6 respectively arranged on the two guide rods, the sliding plates are in sliding fit with the guide rods, and the movable platform is arranged on the sliding plates. The driving mechanism comprises a motor 7 arranged on the fixed platform, a disc 8, a transmission pin 9 and a transmission plate 10, wherein the middle part of the disc 8 is fixed on a power output shaft of the motor, the transmission pin 9 is arranged on the edge of the disc, the transmission plate 10 is arranged at the bottom of the movable platform, a vertical straight groove 11 is formed in the middle part of the transmission plate, the transmission pin is inserted into the straight groove and is in sliding fit with the straight groove, and the axial direction of the power output shaft of the motor is perpendicular to the axial direction of the guide rod; and a fixing mechanism for fixing the culture bottle is arranged on the top surface of the moving platform.

In this embodiment, the fixing mechanism is an elastic clip 12, and three or four elastic clips are arranged around each culture bottle. Of course, the described fixing mechanism may also take other forms in different embodiments.

In aerobic microbe's cultivation in-process, place the blake bottle in the elasticity clamping piece, then starter motor, the motor drive disc is rotatory, and the rotatory driving pin that makes on it of disc is the motion of turning to, and the driving pin slides in the straight flute on the driving plate simultaneously at the rotation in-process to reciprocating motion about the driving pin promotes the driving plate, and then reciprocating motion about making moving platform, can play the purpose that makes the blake bottle in the culture medium vibration like this. The culture medium oscillation can accelerate the dissolution of oxygen in the air into the culture medium so as to ensure the supply of dissolved oxygen in the culture medium and improve the culture efficiency of aerobic microorganisms.

As an improvement to the above embodiment, the culture flask comprises a flask body 101, a flask bottom 102, a microbial filter membrane 103, a support plate 104 with a hole, a first seal ring 105 and a second seal ring 106, wherein the lower end of the flask body is connected with the upper end of the flask bottom through a thread, an annular flange 107 is arranged on the inner wall of the lower end of the flask body, and the first seal ring, the microbial filter membrane, the support plate and the second seal ring are sequentially arranged between the annular flange and the upper end face of the flask bottom from top to bottom.

The side wall of the bottle bottom is provided with a pipe connector 108 and a sealing cover 109 arranged on the pipe connector.

When the device is used, the sealing cover 109 is pulled out, the pipe connector at the bottom of the bottle is connected with a suction pump through a hose, liquid to be filtered is led into a microorganism oscillation culture device, and the suction pump is started to finish filtering; the first sealing washer and the second sealing washer can guarantee that filtrating can not reveal from the joint portion of bottle end and body in the filtering process. And after the filtration is finished, covering the sealing cover on the pipe joint again, and introducing the culture medium into a microorganism shaking culture device to culture the microorganisms enriched on the filter membrane.

The blake bottle possesses the function of filtering microorganism and cultivateing the microorganism simultaneously in this embodiment for after filtering the enrichment microorganism through the filter membrane, can directly carry out the microorganism culture to adding liquid culture medium in the blake bottle, no longer need shift the filter membrane, simplified the microorganism culture operation process greatly, improved microorganism culture work efficiency, and avoided shifting the pollution risk that the filter membrane exists.

Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.

Claims

1. The utility model provides a microorganism shake culture apparatus, includes the blake bottle, its characterized in that: the culture bottle reciprocating motion device comprises a culture bottle, and is characterized by further comprising an oscillator for driving the culture bottle to reciprocate, wherein the oscillator comprises a fixed platform, a movable platform, a guide structure and a driving mechanism, the guide structure comprises a support arranged on the fixed platform, two guide rods which are horizontally arranged on the support and parallel to each other, and sliding plates respectively arranged on the two guide rods, the sliding plates are in sliding fit with the guide rods, and the movable platform is arranged on the sliding plates; the driving mechanism comprises a motor arranged on the fixed platform, a disc, a transmission pin and a transmission plate, wherein the middle part of the disc is fixed on a power output shaft of the motor, the transmission pin is arranged on the edge of the disc, the transmission plate is arranged at the bottom of the movable platform, a vertical straight groove is formed in the middle part of the transmission plate, the transmission pin is inserted into the straight groove and is in sliding fit with the straight groove, and the axial direction of the power output shaft of the motor is perpendicular to the axial direction of the guide rod; and a fixing mechanism for fixing the culture bottle is arranged on the top surface of the moving platform.

2. The microbial shaking culture device of claim 1, wherein: the fixing mechanism is an elastic clamping piece.

3. The microbial shaking culture device of claim 1, wherein: the culture bottle comprises a bottle body, a bottle bottom, a microbial filter membrane, a supporting plate with holes, a first sealing ring and a second sealing ring, wherein the lower end of the bottle body is connected with the upper end of the bottle bottom through threads, an annular flange is arranged on the inner wall of the lower end of the bottle body, and the first sealing ring, the microbial filter membrane, the supporting plate and the second sealing ring are sequentially arranged between the annular flange and the upper end face of the bottle bottom from top to bottom;