CN208829676U - A kind of device for realizing the transfection of cationic polymer method - Google Patents
A kind of device for realizing the transfection of cationic polymer method Download PDFInfo
- Publication number
- CN208829676U CN208829676U CN201820919314.5U CN201820919314U CN208829676U CN 208829676 U CN208829676 U CN 208829676U CN 201820919314 U CN201820919314 U CN 201820919314U CN 208829676 U CN208829676 U CN 208829676U
- Authority
- CN
- China
- Prior art keywords
- transfection
- bottle
- realizing
- filter
- cationic polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001890 transfection Methods 0.000 title claims abstract description 84
- 238000000034 method Methods 0.000 title claims abstract description 32
- 229920006317 cationic polymer Polymers 0.000 title claims abstract description 20
- 239000000872 buffer Substances 0.000 claims abstract description 40
- 239000012096 transfection reagent Substances 0.000 claims abstract description 35
- 239000013612 plasmid Substances 0.000 claims abstract description 34
- 238000003756 stirring Methods 0.000 claims abstract description 28
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 20
- 230000002572 peristaltic effect Effects 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 239000002131 composite material Substances 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 230000001413 cellular effect Effects 0.000 claims description 3
- 230000003321 amplification Effects 0.000 abstract description 4
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 4
- 230000009897 systematic effect Effects 0.000 abstract description 2
- 238000004500 asepsis Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 27
- 238000003760 magnetic stirring Methods 0.000 description 17
- 238000013019 agitation Methods 0.000 description 9
- 238000001816 cooling Methods 0.000 description 8
- 229920002873 Polyethylenimine Polymers 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- 239000001506 calcium phosphate Substances 0.000 description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 description 5
- 235000011010 calcium phosphates Nutrition 0.000 description 5
- 210000000170 cell membrane Anatomy 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 108091029865 Exogenous DNA Proteins 0.000 description 4
- 239000012930 cell culture fluid Substances 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000002502 liposome Substances 0.000 description 4
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000013492 plasmid preparation Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000003466 welding Methods 0.000 description 3
- 241000702421 Dependoparvovirus Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000002659 cell therapy Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001415 gene therapy Methods 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 229920001601 polyetherimide Polymers 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- CREXVNNSNOKDHW-UHFFFAOYSA-N azaniumylideneazanide Chemical group N[N] CREXVNNSNOKDHW-UHFFFAOYSA-N 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000002121 endocytic effect Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000006068 polycondensation reaction Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 208000037922 refractory disease Diseases 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 238000003146 transient transfection Methods 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
Abstract
The utility model relates to a kind of devices for realizing the transfection of cationic polymer method; described device includes setting gradually to be connected to by connecting pipe; for preparing the preparation bottle of plasmid buffer; for preparing the preparation bottle of transfection reagent buffer; for carrying out the filter of aseptic filtration; for realizing the stirring rotator of stirring, for mixing and realizing the transfection bottle of transfection.Utility model device saves manpower consumption, realize the batch operation of semi-automatic even automation, it reduces manual operation process and causes systematic error, common Clean Operating Lab can open-sky technique, integral asepsis avoids pollution, cell transfecting efficiency obtained is high, and stability is good, is especially suitable for the amplification of carry out scale and application.
Description
Technical field
The utility model relates to a kind of realization rapid cellulars to transfect device, be particularly suitable for cationic polymer method and quickly criticize
The device of amount transfection cell.
Background technique
Cell therapy and gene therapy bring completely new breakthrough to the control of many refractory diseases, are biological skills in recent years
Direction the swiftest and the most violent is developed in art field.Important carrying form of the viral vectors as cell therapy and gene therapy, big rule
The production method that mould production relies primarily on transient transfection at present obtains.
Transfection (Transfection) is that exogenous genetic material (DNA or RNA) is implanted into a kind of process of cell, common
Mode has non-chemical property transfection and chemically transfects.Non-chemical property transfection has following methods: electroporation, i.e., when cell is sudden and violent
It is exposed in short pulse caused by strong electrical field, permeability of cell membranes can be instantly increased, and cell is enable to absorb foreign gene；Cell
Extrusion gets higher the permeability on cell membrane by mild compression cell membrane, to reach the mesh of intake foreign gene
's；Acoustic horn effect is small on inducing cell film by the bubble and neighbouring cell membrane interaction of high-intensitive ultrasonic formation
The formation in hole.Chemically transfection has: liposome method, and liposome is small-sized, spherical, monofilm a fat construction, and inside can be with
Exogenous DNA is encased, when liposome is placed into cell, it will cell membrane is incorporated, so that internal exogenous DNA be discharged into carefully
In born of the same parents；Calcium phosphate method, this mode need the HEPES buffer solution with phosphate radical and the calcium chloride solution with exogenous DNA, when
Both solution are uniformly mixed, positively charged calcium ion will combine with electronegative phosphate radical and form calcium phosphate, and external source
DNA can be then bonded on the surface of calcium phosphate, this suspended matter is subsequently added in Tissue Culture Dish (usually with the thin of monolayer growth
Born of the same parents' culture dish), cell will absorb exogenous DNA；Cationic polymer method, negatively charged DNA are incorporated on polycation,
It is absorbed again by cell row encytosis.The rotaring transfecting mode of obvious non-chemical property, needs complicated instrument and equipment, to the sterile of equipment
Property, can amplification require it is high, so being often difficult to use in production-scale transfection.Liposome method, although large fragment can be imported
DNA can transfect various types of cells, but its use cost is excessively high, significantly limits the application of its industrialization.Calcium phosphate method
Cost is relatively low, but it precipitates by cell endocytic, therefore in suspension cell dependent on DNA- calcium phosphate complex and is produced as biological system
The epoch of medicine mainstream are difficult to play practical use.In contrast, cationic polymer transfection is high-efficient, easy to operate, is applicable in model
It encloses extensively, reproducible, cytotoxicity is low, uses particularly suitable for production rank.
The most widely used cationic polymer is polyethyleneimine (Polyethylenimine, PEI), it is a kind of
The organic macromolecule of cationic charge density with higher, every phase next but two carbon atom, i.e., per " third atom is all matter
The amino nitrogen atom of sonization, so that polymer network can serve as effective " proton sponge " (proton at any pH
Sponge) body, as transfection reagent, it can form particle and transfecting eukaryotic cells with polycondensation DNA molecular.
The other cationic polymer transfection method of laboratory level, using vortex oscillator, first by DNA and transfection buffer into
Then PEI is mixed with transfection buffer, then the two is mixed by row mixing.This mode is in superclean bench or bio-safety
It is completed in cabinet, open-sky technique, and relies on vortex oscillator, there are microbiological contamination risks, and scale is difficult to be amplified to production.Cause
This, one kind can avoid pollution risk, and the cationic polymer transfection mode for capableing of Linear Amplifer becomes the urgent of the field and is essential
It asks.
It is connected by reasonable pipeline, container, cooperates the use of sterile filters, which is D grades or more in cleanliness
Opening manipulation is realized in region, under the premise of not influencing transfection efficiency, configuration that is simple, uniform, being efficiently completed rotaring redyeing system,
And the risk for avoiding pollution realizes the transfection production of extensive suspension cell.
Summary of the invention
The technical issues of the utility model is solved be overcome the deficiencies of the prior art and provide one kind can it is semi-automatic,
Automation, the device for steadily realizing cell transfecting efficiently, in batches.
The utility model adopts the following technical solution:
A kind of device for realizing the transfection of cationic polymer method, described device include that plasmid prepares bottle, transfection reagent
Bottle, transfection bottle, peristaltic pump, filter, stirring rotator, breather filter are prepared, the breather filter keeps bottle for gas exchanges
Inside and outside gas pressure is consistent, and the filter is used for aseptic filtration, and the plasmid prepares bottle for mixing transfection cells with plasmids
With buffer, the transfection reagent prepares bottle for mixing transfection reagent and buffer, and the stirring rotator is for realizing liquid
It mixes, the peristaltic pump is that the liquid for realizing preparing two in bottle is delivered to transfection bottle, and the transfection bottle is for mixing
Plasmid buffer and transfection reagent buffer realize the preparation of transfection composite.
Further, the transfection liquid reagent is cationic polymer.
Preferably, the cationic polymer is polyethyleneimine (Polyethylenimine, PEI).
Further, the plasmid is transfection cell recombinant plasmid.
Further, the mixed proportion of the buffer and plasmid, buffer: plasmid ratio is 50:1~50:3.
Further, the mixed proportion of the buffer and transfection reagent (mass concentration 0.1%), buffer: transfection
Ratio of reagents is 50:2~50:9.
Further, the filter is 0.22um bacterial filter.
Further, the breather filter is 0.22um breather filter.
One step of ground, the stirring rotator is high-temperature resistant polytetrafluoroethylmelt magnetic stir bar, and cooperation magnetic stirring apparatus uses,
Agitation revolution is 50~200rpm.
Further, the wriggling revolution speed is 50~200rpm.
Further, the transfection process is that transfection liquid is mixed with cell culture.
Preferably, the mixed volume ratio of cell culture fluid and transfection composite liquid is 10:1~20:1.
The transfection device further includes amplifying for plasmid buffer tank used in scale amplification transfection, for scale
Transfection transfection reagent buffer tank used, for the transfection tank of scale magnocell transfection, for will be between described device
The mechanical pump of liquid conveying, and be connected to the preparing tank and transfect the delivery pipe between tank, liquid filtering filter used
And the stirrer for being stirred.
Further, the preparing tank be can high pressure sterilization and the fermentor containing cooling device and agitating device.
Further, the transfection tank be can high pressure sterilization and the fermentor containing cooling device and agitating device.
Using the cell transfecting device of the utility model and in conjunction with transfection conditions appropriate, it can be obtained stable cell and turn
Contaminating efficiency is 90~95%.
Due to the implementation of above-mentioned technical proposal, the utility model technology has following excellent compared with existing manual operation technology
Point:
Using utility model device, and technological parameter appropriate and condition are selected, can be obtained to meet and stable be more than
The cell transfecting efficiency of manual operation.Utility model device saves manpower consumption, realizes semi-automatic even automation
Batch operation, reduce manual operation process and cause systematic error, common Clean Operating Lab can open-sky technique, completely
Sterile to avoid pollution, cell transfecting efficiency obtained is high, and stability is good, is especially suitable for the amplification of carry out scale and application.
Detailed description of the invention
Fig. 1: apparatus structure schematic diagram
Diagram letter and title represented by number are as follows:
A, plasmid prepares bottle；B, bottle is transfected；C, transfection reagent prepares bottle
1, breather filter；2, filter；3, peristaltic pump；4, stirring rotator
Specific embodiment
The utility model is further described in conjunction with Figure of description.
As described in Figure 1, a kind of device for realizing the transfection of cationic polymer method, including plasmid prepare bottle A, transfect bottle
B, transfection reagent prepare bottle C, breather filter 1, filter 2, peristaltic pump 3, stirring rotator 4；It is for mixing that the plasmid, which prepares bottle A,
Close transfection cells with plasmids and buffer, it is described turn for mixing transfection reagent and buffer that the transfection reagent, which prepares bottle C,
Dye bottle B is used to mix the mixing plasmid buffer from A and realizes matching for transfection composite with the transfection reagent buffer from C
System；The breather filter 1 keeps gas pressure inside and outside bottle consistent for gas exchanges, and the filter 2 is used for aseptic filtration, institute
Stirring rotator 4 is stated for realizing liquid blending, the peristaltic pump 3 is that the liquid for realizing preparing two in bottles is delivered to and turns
Contaminate bottle.
Breather filter 1 is 0.22um breather filter.
Filter 2 is 0.22um bacterial filter.
3 revolving speed of peristaltic pump is 50~200rpm.
Stirring rotator 4 is high-temperature resistant polytetrafluoroethylmelt magnetic stir bar, and cooperation magnetic stirring apparatus uses, agitation revolution 50
~200rpm.
The step of laboratory rapid batch cell transfecting is realized using utility model device is as follows: connecting first by diagram
Adapter tube road, filter prepare bottle and transfection bottle, and stirring rotator is placed in each bottle, by 121 DEG C of high pressure moist heat sterilization 20- of package unit
30min, cooling are spare.Or connect the pipeline between A and B, between B and C using solderable pipeline, by each section independent 121
DEG C high pressure moist heat sterilization 20-30min, carried out after cooling sterile welded connecting be integrated it is spare as shown.It opens plasmid and prepares bottle A
The desired amount of buffer is added thereto by bottle cap, then the desired amount of expression plasmid is added thereto, buffer: plasmid ratio is
Plasmid preparation bottle A bottle cap is screwed and is placed on magnetic stirring apparatus by 50:1~50:3, and agitation revolution is set as 50~200rpm,
After stirring 1-10min, magnetic stirring apparatus is closed, plasmid is prepared into the plasmid buffer in bottle A via degerming using peristaltic pump 3
Filter 2 is pumped into transfection bottle B.It opens transfection reagent and prepares bottle C bottle cap, the desired amount of buffer is added thereto, then by aequum
Transfection reagent (mass concentration 0.1%) be added thereto, buffer: transfection reagent ratio be 50:2~50:9, will transfection examination
Agent preparation bottle C bottle cap, which screws, to be placed on magnetic stirring apparatus, and agitation revolution is set as 50~200rpm, after stirring 1-10min, closes
Magnetic stirring apparatus is closed, transfection bottle B is placed on magnetic stirring apparatus, agitation revolution is set as 50~200rpm, uses peristaltic pump 3
Transfection reagent is prepared into the transfection reagent buffer in bottle C and is pumped into transfection bottle B via sterilizing filter 2.Prepare in transfection bottle B
Good transfection composite liquid can be by the sterile welded pipe line on transfection bottle B, by sterile welding technique, for any volume
The transfection of cell fermentation liquid use (the mixed volume ratio of cell culture fluid and transfection composite liquid be 10:1~20:1).
Realize that the step of laboratory rapid batch cell transfecting produces slow virus is as follows using utility model device: first
First by diagram connecting pipe, filter, preparation bottle and transfection bottle, stirring rotator is placed in each bottle, and 121 DEG C of high pressures of package unit are wet
Heat sterilization 20-30min, cooling are spare.Or the pipeline between A and B, between B and C is connected using solderable pipeline, Jiang Gebu
Point independent 121 DEG C of high pressures moist heat sterilization 20-30min, carried out after cooling sterile welded connecting be integrated it is spare as shown.Open matter
Grain prepares bottle A bottle cap, the desired amount of buffer is added thereto, then it is added in the desired amount of slow virus plasmid packaging system
In, buffer: plasmid ratio is 50:1~50:3, and plasmid preparation bottle A bottle cap is screwed and is placed on magnetic stirring apparatus, stirring turns
Number is set as 50~200rpm, after stirring 1-10min, closes magnetic stirring apparatus, is prepared plasmid in bottle A using peristaltic pump 3
Plasmid buffer is pumped into transfection bottle B via sterilizing filter 2.It opens transfection reagent and prepares bottle C bottle cap, by the desired amount of buffer
Be added thereto, then the desired amount of transfection reagent (mass concentration 0.1%) be added thereto, buffer: transfection reagent ratio is
Transfection reagent preparation bottle C bottle cap is screwed and is placed on magnetic stirring apparatus by 50:2~50:9, and agitation revolution is set as 50~
200rpm after stirring 1-10min, closes magnetic stirring apparatus, and transfection bottle B is placed on magnetic stirring apparatus, agitation revolution setting
For 50~200rpm, transfection reagent is prepared into the transfection reagent buffer in bottle C using peristaltic pump 3 and is pumped via sterilizing filter 2
Enter to transfect bottle B.Ready transfection composite liquid can be led to by the sterile welded pipe line on transfection bottle B in transfection bottle B
Cross sterile welding technique, for any volume the transfection of cell fermentation liquid use (cell culture fluid and transfection composite liquid it is mixed
Conjunction volume ratio is 10:1~20:1).
The step of laboratory rapid batch cell transfecting produces adeno-associated virus is realized using utility model device such as
Under: first by diagram connecting pipe, filter, preparation bottle and transfection bottle, stirring rotator is placed in each bottle, by 121 DEG C of package unit
High pressure moist heat sterilization 20-30min, cooling are spare.Or connect the pipeline between A and B, between B and C using solderable pipeline,
By the independent 121 DEG C of high pressures moist heat sterilization 20-30min of each section, carried out after cooling sterile welded connecting be integrated it is spare as shown.
It opens plasmid and prepares bottle A bottle cap, the desired amount of buffer is added thereto, then the desired amount of adeno-associated virus plasmid is packed and is
System is added thereto, buffer: plasmid ratio is 50:1~50:3, and plasmid preparation bottle A bottle cap is screwed and is placed on magnetic stirring apparatus
On, agitation revolution is set as 50~200rpm, after stirring 1-10min, closes magnetic stirring apparatus, is matched plasmid using peristaltic pump 3
Plasmid buffer in bottle A processed is pumped into transfection bottle B via sterilizing filter 2.It opens transfection reagent and prepares bottle C bottle cap, it will be required
The buffer of amount is added thereto, then the desired amount of transfection reagent (mass concentration 0.1%) is added thereto, buffer: transfection
Ratio of reagents is 50:2~50:9, and transfection reagent preparation bottle C bottle cap is screwed and is placed on magnetic stirring apparatus, agitation revolution setting
For 50~200rpm, after stirring 1-10min, magnetic stirring apparatus is closed, transfection bottle B is placed on magnetic stirring apparatus, stirring turns
Number is set as 50~200rpm, and transfection reagent is prepared the transfection reagent buffer in bottle C via aseptic filtration using peristaltic pump 3
Device 2 is pumped into transfection bottle B.Ready transfection composite liquid can pass through the sterile welded pipe on transfection bottle B in transfection bottle B
Road, by sterile welding technique, the cell fermentation liquid transfection for any volume uses (cell culture fluid and transfection composite liquid
Mixed volume ratio be 10:1~20:1).
Claims (7)
1. a kind of device for realizing the transfection of cationic polymer method, it is characterised in that: prepare bottle, transfection reagent including plasmid
Bottle, transfection bottle, peristaltic pump, filter, stirring rotator, breather filter are prepared, the breather filter keeps bottle for gas exchanges
Inside and outside gas pressure is consistent, and the filter is used for aseptic filtration, and the plasmid prepares bottle for mixing transfection cells with plasmids
With buffer, the transfection reagent prepares bottle for mixing transfection reagent and buffer, and the stirring rotator is for realizing liquid
It mixes, the peristaltic pump is that the liquid for realizing preparing two in bottle is delivered to transfection bottle, and the transfection bottle is for mixing
Plasmid buffer and transfection reagent buffer realize the preparation of transfection composite.
2. the device according to claim 1 for realizing the transfection of cationic polymer method, it is characterised in that: the plasmid is matched
Between bottle and filter processed, transfection reagent prepare between bottle and filter, between filter and transfection bottle for can not welded pipe line.
3. the device according to claim 1 for realizing the transfection of cationic polymer method, it is characterised in that: the plasmid is matched
Between bottle and filter processed, transfection reagent prepares between bottle and filter, between filter and transfection bottle is solderable pipeline.
4. the device according to claim 1 for realizing the transfection of cationic polymer method, it is characterised in that: the preparation bottle
It transfects and uses for laboratory small lot rapid cellular, autoclave sterilization process can be carried out；Scale may be designed as fermentor when amplifying.
5. the device according to claim 1 for realizing the transfection of cationic polymer method, it is characterised in that: the transfection bottle
It transfects and uses for laboratory small lot rapid cellular, autoclave sterilization process can be carried out；Scale may be designed as fermentor when amplifying.
6. the device according to claim 1 for realizing the transfection of cationic polymer method, it is characterised in that: the preparation bottle
To mix sack, agitating mode is that overhead stirs or wave stirs.
7. the device according to claim 1 for realizing the transfection of cationic polymer method, it is characterised in that: the transfection bottle
To mix sack, agitating mode is that overhead stirs or wave stirs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820919314.5U CN208829676U (en) | 2018-06-14 | 2018-06-14 | A kind of device for realizing the transfection of cationic polymer method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820919314.5U CN208829676U (en) | 2018-06-14 | 2018-06-14 | A kind of device for realizing the transfection of cationic polymer method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208829676U true CN208829676U (en) | 2019-05-07 |
Family
ID=66305337
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820919314.5U Active CN208829676U (en) | 2018-06-14 | 2018-06-14 | A kind of device for realizing the transfection of cationic polymer method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208829676U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438162A (en) * | 2019-08-22 | 2019-11-12 | 广州晶欣生物科技有限公司 | A kind of modified form non-liposomal transfection reagent box and its application method |
-
2018
- 2018-06-14 CN CN201820919314.5U patent/CN208829676U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438162A (en) * | 2019-08-22 | 2019-11-12 | 广州晶欣生物科技有限公司 | A kind of modified form non-liposomal transfection reagent box and its application method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pigeau et al. | Commercial scale manufacturing of allogeneic cell therapy | |
| JP6380994B2 (en) | Alternating ion magnetic resonance (AIMR) multi-chamber culture apparatus and method of use thereof | |
| TWI233449B (en) | High efficient cell-cultivating device | |
| EP0938544B1 (en) | Cell/tissue culturing device and method | |
| EP2603517B1 (en) | Dynamic mixing and electroporation chamber and system | |
| JP4866736B2 (en) | System for cell culture | |
| CN108866013B (en) | Method for large-scale production of lentivirus | |
| CN208829676U (en) | A kind of device for realizing the transfection of cationic polymer method | |
| JP2020519258A (en) | A scalable method for generating transfection reagents | |
| JP2009505672A (en) | Semi-continuous fermentation method | |
| CN111560399B (en) | Large-scale transient transfection method for cells | |
| CN207193305U (en) | Doughnut exchanger and doughnut switch type culture systems | |
| CN205115481U (en) | A device for realizing quick transfection cell in batches of calcium phosphate method | |
| CN115261302B (en) | Matrigel and preparation method and application thereof | |
| Karnieli | Bioreactors and downstream processing for stem cell manufacturing | |
| CN110129367A (en) | A kind of cationic transfection reagent and its preparation method and application | |
| CN108977358A (en) | A kind of closed bioreactor and its cell culture processes | |
| CN113667596A (en) | Low-temperature blending device for preparing lentiviral vector and purification method of lentiviral vector | |
| CN208949309U (en) | A kind of automatic cytological culture apparatus for simulating organismic internal environment | |
| CN113832112A (en) | Mixing method and mixing system for preparing lentivirus | |
| Zwerner et al. | Five‐year perspective of the large‐scale growth of mammalian cells in suspension culture | |
| CN220376690U (en) | Large-scale dynamic and static online cell transfection system | |
| CN216764925U (en) | A low temperature mixing device for lentiviral vector preparation | |
| CN207347576U (en) | One kind is used for microcarrier full-automatic digestion facility separated with zooblast | |
| CN219539491U (en) | Continuous flow affinity chromatography transfer device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231028 Address after: Room 3983, Building 2, Lane 1800, Xinyang Road, Lingang New Area, China (Shanghai) Pilot Free Trade Zone, Fengxian District, Shanghai, 2013 Patentee after: Heyuan Zhizao (Shanghai) Gene Technology Co.,Ltd. Address before: 201321 building 19, Lane 908, Ziping Road, international medical park, Pudong New Area, Shanghai Patentee before: OBIO TECHNOLOGY (SHANGHAI) Corp.,Ltd. |
|
| TR01 | Transfer of patent right |