CN112481103B - High-efficient immune cell sampling device - Google Patents
High-efficient immune cell sampling device Download PDFInfo
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- CN112481103B CN112481103B CN202011345695.9A CN202011345695A CN112481103B CN 112481103 B CN112481103 B CN 112481103B CN 202011345695 A CN202011345695 A CN 202011345695A CN 112481103 B CN112481103 B CN 112481103B
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- extraction
- negative pressure
- connecting pipe
- needle head
- baffle
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- 210000002865 immune cell Anatomy 0.000 title claims abstract description 29
- 238000005070 sampling Methods 0.000 title claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 44
- 238000007789 sealing Methods 0.000 claims abstract description 35
- 230000000903 blocking effect Effects 0.000 claims abstract description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 13
- 238000000926 separation method Methods 0.000 abstract description 11
- 210000004027 cell Anatomy 0.000 description 10
- 238000000034 method Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a high-efficiency immune cell sampling device, which comprises negative pressure equipment and extraction equipment, wherein the extraction equipment consists of an extraction needle head, a transparent connecting pipe and a connecting sleeve, the transparent connecting pipe is used for connecting the extraction needle head and the connecting sleeve, the bottom end of the negative pressure equipment is inserted into the connecting sleeve, a grid baffle is arranged in the extraction needle head, the grid baffle slides up and down in a sealing manner in the extraction needle head, the inner diameter of the transparent connecting pipe is consistent with the inner diameter of the extraction needle head, a baffle ring is additionally arranged at the top end of the inner wall of the transparent connecting pipe, the baffle ring is used for blocking the grid baffle from continuously moving upwards, and capacity scale marks are arranged on the outer side of the transparent connecting pipe; through installing the check separation blade additional in the extraction syringe needle, check separation blade and the contact of baffle ring this moment, extraction equipment stops to extract immune cells, and immune cells prevents to get into in the negative pressure equipment under the stop of check separation blade, keeps negative pressure equipment's not polluted.
Description
Technical Field
The utility model relates to a sampling device, in particular to a high-efficiency immune cell sampling device.
Background
Immune cells refer to cells involved in or associated with an immune response, including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells, leukocytes, etc., which may be divided into a variety of classes, and which play an important role in the human body, and which are commonly known as leukocytes, including innate lymphocytes, various phagocytes, etc., and lymphocytes capable of recognizing antigens, producing specific immune responses, etc
In the prior art, the needle tube is connected with the negative pressure equipment to extract immune cells, and the cell sample sampling device disclosed by the utility model with the authorized publication number of CN204542231U, although avoiding the position of the puncture fine needle from shifting in the operation process, further ensuring the accuracy of the cell sucking position, does not solve the problem that cells easily enter the negative pressure equipment and pollute the negative pressure equipment.
Disclosure of Invention
In order to overcome the defects of the prior art, the utility model provides a high-efficiency immune cell sampling device, which can solve the technical problem that cells easily enter negative pressure equipment to pollute the negative pressure equipment.
In order to solve the technical problems, the utility model provides the following technical scheme: the utility model provides a high-efficient immune cell sampling device, includes negative pressure equipment and extraction equipment, extraction equipment comprises extraction syringe needle, transparent connecting pipe and adapter sleeve, transparent connecting pipe is used for connecting extraction syringe needle and adapter sleeve, negative pressure equipment bottom is pegged graft in the adapter sleeve, be equipped with the check separation blade in the extraction syringe needle, the check separation blade is sealed sliding from top to bottom in the extraction syringe needle, transparent connecting pipe internal diameter is unanimous with extraction syringe needle internal diameter, transparent connecting pipe inner wall top department adds establishes the baffle ring, the baffle ring is used for blockking the check separation blade and continues to reciprocate, the transparent connecting pipe outside is equipped with capacity scale mark.
As a preferable technical scheme of the utility model, the outer side of the connecting sleeve is provided with anti-skid patterns for skid prevention at equal intervals.
As a preferable technical scheme of the utility model, rubber sealing rings are additionally arranged at equal intervals on the outer side of the bottom end of the negative pressure equipment, a sealing groove matched with the rubber sealing rings is formed in the inner wall of the connecting sleeve, and the rubber sealing rings are embedded in the sealing groove to be connected in a sealing way.
As a preferable technical scheme of the utility model, the top end of the grid baffle is additionally provided with the auxiliary sheet, and the auxiliary sheet is connected with the grid baffle through the connecting column.
As a preferable technical scheme of the utility model, the auxiliary sheet and the grid baffle are consistent in specification.
As a preferable technical scheme of the utility model, the top end of the auxiliary sheet is additionally provided with a silica gel ring, the bottom end of the baffle ring is provided with a corresponding sealing cavity, and the silica gel ring is embedded in the sealing cavity for sealing connection.
As a preferable technical scheme of the utility model, the two sides of the inner top end and the outer top end of the silica gel ring are provided with inclined planes.
Compared with the prior art, the utility model has the following beneficial effects:
1. through installing the check separation blade in the extraction needle, when the immune cells in the human body need to be sampled, the extraction needle is inserted into the corresponding human body, then negative pressure equipment is started, immune cells in the human body are gradually keyed into the extraction needle under the action of negative pressure at the moment, the check separation blade in the extraction needle is driven to move upwards, the bottom edge points to the capacity scale mark in the moving process of the check separation blade, the medical staff can observe the volume of the extracted cells conveniently, according to actual needs, the immune cells are sampled correspondingly, when the immune cell extraction reaches the maximum capacity, the check separation blade is contacted with the baffle ring at the moment, the extraction equipment stops extracting the immune cells, the immune cells are prevented from entering the negative pressure equipment under the blocking of the check separation blade, and the negative pressure equipment is kept from being polluted;
2. the auxiliary sheet is additionally arranged at the top end of the grid baffle sheet, the auxiliary sheet is connected with the grid baffle sheet through the connecting column, and the grid baffle sheet drives the auxiliary sheet to move through the connecting column in the moving process, so that the grid baffle sheet is prevented from inclining in the moving process, and the moving stability of the grid baffle sheet is further ensured;
3. through installing the silica gel ring additional on auxiliary sheet top to set up corresponding seal chamber in keeping off the ring bottom, when silica gel ring embedding at seal intracavity sealing connection, further promote the sealed effect of auxiliary sheet and keeping off the ring, thereby further reached the effect that stops immune cells and get into in the negative pressure equipment.
Drawings
FIG. 1 is a schematic diagram of a sampling device according to the present utility model;
FIG. 2 is an enlarged schematic view of the structure at a in FIG. 1;
FIG. 3 is an enlarged schematic view of the structure at c in FIG. 2;
FIG. 4 is an enlarged schematic view of the structure at b in FIG. 2;
FIG. 5 is an enlarged schematic view of the structure of FIG. 2 at d;
wherein: 1. a negative pressure device; 2. extracting the needle; 4. a transparent connection pipe; 41. capacity graduation marks; 43. a baffle ring; 3. connecting sleeves; 31. anti-skid lines; 32. a rubber seal ring; 33. sealing grooves; 5. a grid baffle; 51. a connecting column; 52. an auxiliary sheet; 53. a silica gel ring; 54. sealing the cavity.
Detailed Description
In order that the manner in which the above recited features, objects and advantages of the present utility model are obtained will become readily apparent, a more particular description of the utility model will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings. Based on the examples in the embodiments, those skilled in the art can obtain other examples without making any inventive effort, which fall within the scope of the utility model. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.
Example 1
Referring to fig. 1 to 2, the utility model provides a high-efficiency immune cell sampling device, which comprises a negative pressure device 1 and an extraction device, wherein the extraction device consists of an extraction needle 2, a transparent connecting pipe 4 and a connecting sleeve 3, the transparent connecting pipe 4 is used for connecting the extraction needle 2 and the connecting sleeve 3, the bottom end of the negative pressure device 1 is inserted into the connecting sleeve 3, a grid baffle 5 is arranged in the extraction needle 2, the grid baffle 5 slides up and down in a sealing manner in the extraction needle 2, in order to ensure that the grid baffle 5 slides smoothly in the extraction needle 2 and the transparent connecting pipe 4, the inner diameter of the transparent connecting pipe 4 is consistent with the inner diameter of the extraction needle 2, a baffle ring 43 is correspondingly arranged at the top end of the inner wall of the transparent connecting pipe 4, the baffle ring 43 is used for blocking the grid baffle 5 to move upwards, so that when the grid baffle 5 moves to the baffle ring 43, the cells in the extraction needle 2 are stopped from moving upwards under the blocking of the grid baffle 5, a capacity scale mark 41 is arranged outside the transparent 4 when a sampling person observes the volume of the extracted cells, the corresponding capacity of the transparent connecting pipe 4 is pointed to the corresponding position of the transparent connecting pipe, and the sampling device can be marked with the practical requirements according to the sample size of the corresponding cell.
When the device is used, when immune cells in a human body need to be sampled, the extraction needle head 2 is inserted into a corresponding human body, then the negative pressure device 1 is started, immune cells in the human body are gradually keyed into the extraction needle head 2 under the action of negative pressure, meanwhile, the grid baffle 5 in the extraction needle head 2 is driven to move upwards, the bottom edge points to the capacity scale mark 41 in the moving process of the grid baffle 5, medical staff can observe the volume of the extracted cells conveniently, according to actual needs, the immune cells are sampled correspondingly, when the immune cell extraction reaches the maximum capacity, the grid baffle 5 is contacted with the baffle ring 43, the extraction device stops extracting the immune cells, the immune cells are prevented from entering the negative pressure device 1 under the blocking of the grid baffle 5, and the negative pressure device 1 is kept from being polluted.
Referring to fig. 3, anti-slip patterns 31 for preventing slipping are equidistantly formed on the outer side of the connecting sleeve 3, when the connecting sleeve 3 is separated from the connecting sleeve, the anti-slip patterns 31 can increase the moving friction force between the hand and the connecting sleeve 3, so that the connecting sleeve 3 can be removed more easily, when the connecting sleeve 3 is further improved in tightness with the negative pressure equipment 1, rubber sealing rings 32 are equidistantly arranged on the outer side of the bottom end of the negative pressure equipment 1, sealing grooves 33 matched with the rubber sealing rings 32 are formed on the inner wall of the connecting sleeve 3, and the rubber sealing rings 32 are embedded in the sealing grooves 33 for sealing connection.
Example 2
Referring to fig. 4, the present utility model provides a high-efficiency immune cell sampling device, which adopts the same technical scheme as the first embodiment, and is different in that, in order to prevent the situation that the grid baffle 5 tilts at the extraction needle 2, and thus the immune cells cannot be blocked from entering the negative pressure device 1, an auxiliary sheet 52 is additionally arranged at the top end of the grid baffle 5, and the auxiliary sheet 52 is connected with the grid baffle 5 through a connecting post 51, so that the grid baffle 5 is prevented from tilting in the moving process, and the moving stability of the grid baffle 5 is further ensured, and preferably, the specification of the auxiliary sheet 52 is consistent with that of the grid baffle 5.
Example 3
Referring to fig. 4 and 5, the present utility model provides a high-efficiency immune cell sampling device, which adopts the same technical scheme as the second embodiment, except that in order to further improve the sealing effect of the auxiliary sheet 52 and the baffle ring 43, a silica gel ring 53 is additionally installed at the top end of the auxiliary sheet 52, and a corresponding sealing cavity 54 is opened at the bottom end of the baffle ring 43, the silica gel ring 53 is embedded in the sealing cavity 54 for sealing connection, so as to further achieve the effect of blocking immune cells from entering the negative pressure device 1, and preferably, in order to facilitate the silica gel ring 53 to enter the sealing cavity 54, both sides of the inner and outer top ends of the silica gel ring 53 are provided with inclined surfaces.
In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present utility model, and are not intended to limit the utility model, and that various changes and modifications may be made therein without departing from the spirit and scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (1)
1. The utility model provides a high-efficient immune cell sampling device, includes negative pressure equipment (1) and extraction equipment, its characterized in that: the extraction equipment consists of an extraction needle head (2), a transparent connecting pipe (4) and a connecting sleeve (3), wherein the transparent connecting pipe (4) is used for connecting the extraction needle head (2) and the connecting sleeve (3), the bottom end of the negative pressure equipment (1) is inserted into the connecting sleeve (3), a grid baffle (5) is arranged in the extraction needle head (2), the grid baffle (5) slides in an up-down sealing manner in the extraction needle head (2), the inner diameter of the transparent connecting pipe (4) is consistent with the inner diameter of the extraction needle head (2), a baffle ring (43) is additionally arranged at the top end of the inner wall of the transparent connecting pipe (4), the baffle ring (43) is used for blocking the grid baffle (5) from continuously moving upwards, and capacity scale marks (41) are arranged on the outer side of the transparent connecting pipe (4);
anti-skid patterns (31) for skid resistance are equidistantly formed on the outer side of the connecting sleeve (3);
rubber sealing rings (32) are additionally arranged at equal intervals on the outer side of the bottom end of the negative pressure equipment (1), sealing grooves (33) matched with the rubber sealing rings (32) are formed in the inner wall of the connecting sleeve (3), and the rubber sealing rings (32) are embedded into the sealing grooves (33) to be connected in a sealing mode;
an auxiliary sheet (52) is additionally arranged at the top end of the grid baffle sheet (5), and the auxiliary sheet (52) is connected with the grid baffle sheet (5) through a connecting column (51);
the auxiliary sheet (52) is consistent with the grid baffle (5) in specification;
a silica gel ring (53) is additionally arranged at the top end of the auxiliary sheet (52), a corresponding sealing cavity (54) is formed at the bottom end of the baffle ring (43), and the silica gel ring (53) is embedded in the sealing cavity (54) and is connected in a sealing way;
the two sides of the inner top end and the outer top end of the silica gel ring (53) are provided with inclined planes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011345695.9A CN112481103B (en) | 2020-11-26 | 2020-11-26 | High-efficient immune cell sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011345695.9A CN112481103B (en) | 2020-11-26 | 2020-11-26 | High-efficient immune cell sampling device |
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| Publication Number | Publication Date |
|---|---|
| CN112481103A CN112481103A (en) | 2021-03-12 |
| CN112481103B true CN112481103B (en) | 2023-11-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011345695.9A Active CN112481103B (en) | 2020-11-26 | 2020-11-26 | High-efficient immune cell sampling device |
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| Country | Link |
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| CN (1) | CN112481103B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201814582U (en) * | 2010-10-25 | 2011-05-04 | 孙玉芹 | Anti-pollution check blood taking needle |
| CN104758008A (en) * | 2014-01-03 | 2015-07-08 | 黄海涛 | Biological tissue puncture device and biological tissue puncture method |
| CN204542231U (en) * | 2015-04-13 | 2015-08-12 | 李俊华 | A kind of cell sample sampler |
| CN210736761U (en) * | 2019-09-11 | 2020-06-12 | 山西医科大学汾阳学院 | Immune cell sampling device |
| CN211862863U (en) * | 2019-11-29 | 2020-11-06 | 宋晓霞 | Prevent blockking up tumour biopsy sampler |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2952069B1 (en) * | 2009-11-04 | 2013-06-28 | Metagenex | DEVICE AND METHOD FOR ISOLATING AND / OR CULTIVATING LIVING CELLS ON FILTER OR EXTRACTING GENETIC MATERIAL |
-
2020
- 2020-11-26 CN CN202011345695.9A patent/CN112481103B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201814582U (en) * | 2010-10-25 | 2011-05-04 | 孙玉芹 | Anti-pollution check blood taking needle |
| CN104758008A (en) * | 2014-01-03 | 2015-07-08 | 黄海涛 | Biological tissue puncture device and biological tissue puncture method |
| CN204542231U (en) * | 2015-04-13 | 2015-08-12 | 李俊华 | A kind of cell sample sampler |
| CN210736761U (en) * | 2019-09-11 | 2020-06-12 | 山西医科大学汾阳学院 | Immune cell sampling device |
| CN211862863U (en) * | 2019-11-29 | 2020-11-06 | 宋晓霞 | Prevent blockking up tumour biopsy sampler |
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| Publication number | Publication date |
|---|---|
| CN112481103A (en) | 2021-03-12 |
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