CN112481103A - High-efficient immune cell sampling device - Google Patents
High-efficient immune cell sampling device Download PDFInfo
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- CN112481103A CN112481103A CN202011345695.9A CN202011345695A CN112481103A CN 112481103 A CN112481103 A CN 112481103A CN 202011345695 A CN202011345695 A CN 202011345695A CN 112481103 A CN112481103 A CN 112481103A
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- extraction
- negative pressure
- connecting pipe
- ring
- pressure equipment
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- 210000002865 immune cell Anatomy 0.000 title claims abstract description 35
- 238000005070 sampling Methods 0.000 title claims abstract description 17
- 238000000605 extraction Methods 0.000 claims abstract description 46
- 238000007789 sealing Methods 0.000 claims abstract description 27
- 238000000926 separation method Methods 0.000 claims abstract description 17
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 13
- 230000006978 adaptation Effects 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 abstract description 19
- 230000004888 barrier function Effects 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 11
- 238000000034 method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 230000028993 immune response Effects 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 206010063659 Aversion Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a high-efficiency immune cell sampling device, which comprises negative pressure equipment and extraction equipment, wherein the extraction equipment consists of an extraction needle head, a transparent connecting pipe and a connecting sleeve, the transparent connecting pipe is used for connecting the extraction needle head and the connecting sleeve, the bottom end of the negative pressure equipment is inserted in the connecting sleeve, a grid block sheet is arranged in the extraction needle head, the grid block sheet slides up and down in the extraction needle head in a sealing manner, the inner diameter of the transparent connecting pipe is consistent with that of the extraction needle head, a blocking ring is additionally arranged at the top end of the inner wall of the transparent connecting pipe and is used for blocking the grid block sheet from continuously moving upwards, and capacity scale marks are arranged on the; through install the check separation blade additional in the extraction syringe needle, check separation blade and fender ring contact this moment, extraction equipment stops to extract immune cell, and immune cell is under the blockking of check separation blade, avoids getting into negative pressure equipment in, keeps not contaminated of negative pressure equipment.
Description
Technical Field
The invention relates to a sampling device, in particular to a high-efficiency immune cell sampling device.
Background
Immune cells refer to cells involved in or associated with an immune response, including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells, leukocytes, etc., and the immune cells can be divided into various types, and various immune cells play an important role in the human body, and the immune cells are commonly called leukocytes, including innate lymphocytes, various phagocytic cells, etc., and lymphocytes capable of recognizing antigens and generating specific immune responses, etc
Be connected through needle tubing and negative pressure equipment among the prior art, extract immune cell, be CN 204542231U's utility model discloses a cell sample sampling device if the authorization notice number, it has avoided puncturing fine needle position in operation process and has taken place the aversion, and then has guaranteed the accuracy that the cell absorbs the position, but does not solve the cell and get into in the negative pressure equipment easily, causes the pollution to negative pressure equipment.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a high-efficiency immune cell sampling device which can solve the technical problem that cells easily enter negative pressure equipment to pollute the negative pressure equipment.
In order to solve the technical problems, the invention provides the following technical scheme: the utility model provides a high-efficient immune cell sampling device, includes negative pressure equipment and extraction equipment, extraction equipment comprises extraction syringe needle, transparent connecting pipe and adapter sleeve, transparent connecting pipe is used for connecting extraction syringe needle and adapter sleeve, negative pressure equipment bottom is pegged graft in the adapter sleeve, be equipped with check separation blade in the extraction syringe needle, the sealed slip from top to bottom of check separation blade in the extraction syringe needle, transparent connecting pipe internal diameter is unanimous with the extraction syringe needle internal diameter, transparent connecting pipe inner wall top department adds and establishes the fender ring, it is used for blockking that the check separation blade continues to shift up to keep off the ring, the transparent connecting pipe outside is equipped with the capacity scale mark.
As a preferable technical scheme of the invention, the outer side of the connecting sleeve is equidistantly provided with anti-skid grains for skid resistance.
As a preferred technical scheme of the invention, rubber sealing rings are additionally arranged on the outer side of the bottom end of the negative pressure equipment at equal intervals, a sealing groove matched with the rubber sealing rings is formed in the inner wall of the connecting sleeve, and the rubber sealing rings are embedded in the sealing groove and are connected in a sealing manner.
As a preferable technical scheme of the invention, the top end of the grid baffle sheet is additionally provided with an auxiliary sheet, and the auxiliary sheet is connected with the grid baffle sheet through a connecting column.
As a preferred technical scheme of the invention, the auxiliary sheet is consistent with the specification of the grid separation sheet.
As a preferred technical scheme of the invention, the top end of the auxiliary sheet is additionally provided with a silica gel ring, the bottom end of the baffle ring is provided with a corresponding sealing cavity, and the silica gel ring is embedded in the sealing cavity for sealing connection.
As a preferred technical scheme of the invention, the two sides of the top ends inside and outside the silica gel ring are provided with inclined planes.
Compared with the prior art, the invention can achieve the following beneficial effects:
1. by additionally arranging the grid blocking piece in the extraction needle head, when immune cells in a human body need to be sampled, the extraction needle head is firstly inserted into the corresponding human body, then the negative pressure equipment is started, the immune cells in the human body are gradually keyed into the extraction needle head under the action of negative pressure, meanwhile, the grid blocking piece in the extraction needle head is driven to move upwards, the bottom edge points to the volume scale mark in the moving process of the grid blocking piece, medical personnel can conveniently observe the volume of the extracted cells, the immune cells are correspondingly sampled according to actual needs, when the extraction of the immune cells reaches the maximum volume, the grid blocking piece is in contact with the blocking ring, the extraction equipment stops extracting the immune cells, the immune cells are prevented from entering the negative pressure equipment under the blocking of the grid blocking piece, and the negative pressure equipment is kept from being polluted;
2. the auxiliary sheet is additionally arranged at the top end of the grid blocking sheet, the auxiliary sheet and the grid blocking sheet are connected through the connecting column, and the grid blocking sheet drives the auxiliary sheet to move through the connecting column in the moving process, so that the grid blocking sheet is prevented from inclining in the moving process, and the moving stability of the grid blocking sheet is further guaranteed;
3. through installing the silica gel ring additional on supplementary piece top to offer corresponding seal chamber in keeping off the ring bottom, embedding at seal chamber internal sealing connection when the silica gel ring, further promote supplementary piece and the sealed effect that keeps off the ring, thereby further reached and blocked the effect that immune cell got into in the negative pressure equipment.
Drawings
FIG. 1 is a schematic structural diagram of a sampling device according to the present invention;
FIG. 2 is an enlarged view of a portion a of FIG. 1;
FIG. 3 is an enlarged view of the structure at c in FIG. 2;
FIG. 4 is an enlarged view of the structure at b in FIG. 2;
FIG. 5 is an enlarged view of the structure at d in FIG. 2;
wherein: 1. negative pressure equipment; 2. extracting the needle head; 4. a transparent connecting tube; 41. capacity scale lines; 43. a baffle ring; 3. connecting sleeves; 31. anti-skid lines; 32. a rubber seal ring; 33. a sealing groove; 5. a grid block sheet; 51. connecting columns; 52. an auxiliary sheet; 53. a silica gel ring; 54. the chamber is sealed.
Detailed Description
The present invention will be further described with reference to specific embodiments for the purpose of facilitating an understanding of technical means, characteristics of creation, objectives and functions realized by the present invention, but the following embodiments are only preferred embodiments of the present invention, and are not intended to be exhaustive. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1
Referring to fig. 1 to 2, the present invention provides a high efficiency immune cell sampling device, which comprises a negative pressure device 1 and an extraction device, wherein the extraction device is composed of an extraction needle 2, a transparent connection tube 4 and a connection sleeve 3, the transparent connection tube 4 is used for connecting the extraction needle 2 and the connection sleeve 3, the bottom end of the negative pressure device 1 is inserted into the connection sleeve 3, a lattice barrier 5 is arranged in the extraction needle 2, the lattice barrier 5 slides up and down in the extraction needle 2 in a sealing manner, in order to ensure that the lattice barrier 5 slides smoothly in the extraction needle 2 and the transparent connection tube 4, the inner diameter of the transparent connection tube 4 is consistent with the inner diameter of the extraction needle 2, a barrier ring 43 is correspondingly arranged at the top end of the inner wall of the transparent connection tube 4, the barrier ring 43 is used for blocking the lattice barrier 5 from moving upwards, so that when the lattice barrier 5 moves to the barrier ring 43, the cells in the extraction needle 2 also stop moving upwards under the blocking, avoided in the cell gets into negative pressure equipment 1, when observing the volume of extraction cell for the sampling personnel, be equipped with capacity scale mark 41 in the 4 outsides of transparent connecting pipe, check separation blade 5 when 4 inside movements of transparent connecting pipe, corresponding position that indicates that capacity scale mark 41 marks, medical personnel can carry out corresponding sample to the cell according to actual need for the sample work is lighter more.
During the use, when needing to take a sample to the internal immune cell of human body, insert corresponding human in with extraction syringe needle 2 earlier, then start negative pressure equipment 1, the immune cell in the human body is at this moment typed under the effect of negative pressure in extraction syringe needle 2 gradually, drive check separation blade 5 in the extraction syringe needle 2 simultaneously and move up, check separation blade 5 removes the directional capacity scale mark 41 in-process bottom edge, make things convenient for medical personnel to observe the volume of extraction cell, according to actual need, correspondingly take a sample to the immune cell, when the immune cell extraction reaches maximum capacity, check separation blade 5 contacts with fender ring 43 this moment, extraction equipment stops to extract the immune cell, the immune cell is under the blockking of check separation blade 5, avoid getting into in negative pressure equipment 1, keep not contaminated of negative pressure equipment 1.
Please refer to fig. 3, the outer side of the connecting sleeve 3 is equidistantly provided with anti-slip lines 31 for preventing slipping, when the connecting sleeve 3 is separated from the connecting sleeve 3, the anti-slip lines 31 can increase the moving friction force between the hand and the connecting sleeve 3, so that the connecting sleeve 3 can be taken down more easily, in order to further improve the connection sealing performance between the connecting sleeve 3 and the negative pressure device 1, the outer side of the bottom end of the negative pressure device 1 is equidistantly provided with rubber sealing rings 32, the inner wall of the connecting sleeve 3 is provided with a sealing groove 33 matched with the rubber sealing rings 32, and the rubber sealing rings 32 are embedded in the sealing groove 33 for sealing connection.
Example 2
Referring to fig. 4, the present invention provides a high efficiency immune cell sampling device, the embodiment adopts the same technical solution as the first embodiment, except that an auxiliary sheet 52 is additionally installed on the top end of the grid barrier sheet 5 to prevent the grid barrier sheet 5 from tilting at the extraction needle 2 and causing the situation that the immune cells cannot be blocked from entering the negative pressure device 1, and the auxiliary sheet 52 is connected with the grid barrier sheet 5 through a connecting column 51, so as to prevent the grid barrier sheet 5 from tilting during the moving process, further ensuring the moving stability of the grid barrier sheet 5, and preferably, the auxiliary sheet 52 has the same specification as the grid barrier sheet 5.
Example 3
Referring to fig. 4 and 5, the present invention provides a high efficiency immune cell sampling device, the embodiment adopts the same technical solution as the second embodiment, except that when the sealing effect of the auxiliary plate 52 and the blocking ring 43 is further improved, a silica gel ring 53 is additionally installed at the top end of the auxiliary plate 52, a corresponding sealing cavity 54 is formed at the bottom end of the blocking ring 43, the silica gel ring 53 is embedded in the sealing cavity 54 and hermetically connected, so as to further achieve the effect of blocking immune cells from entering the negative pressure device 1, preferably, when the silica gel ring 53 is convenient to enter the sealing cavity 54, both sides of the inner and outer top ends of the silica gel ring 53 are set as inclined planes.
In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. The utility model provides a high-efficient immune cell sampling device, includes negative pressure equipment (1) and extraction equipment, its characterized in that: the extraction equipment comprises extraction syringe needle (2), transparent connecting pipe (4) and adapter sleeve (3), transparent connecting pipe (4) are used for connecting extraction syringe needle (2) and adapter sleeve (3), negative pressure equipment (1) bottom is pegged graft in adapter sleeve (3), be equipped with check separation blade (5) in extraction syringe needle (2), sealed slip about check separation blade (5) is in extraction syringe needle (2), transparent connecting pipe (4) internal diameter is unanimous with extraction syringe needle (2) internal diameter, transparent connecting pipe (4) inner wall top department adds and establishes fender ring (43), keep off ring (43) and be used for keeping off check separation blade (5) and continue to shift up, the transparent connecting pipe (4) outside is equipped with capacity scale mark (41).
2. The device for sampling immune cells of claim 1, wherein: the outer side of the connecting sleeve (3) is equidistantly provided with anti-skid lines (31) for skid resistance.
3. The device for sampling immune cells of claim 1, wherein: negative pressure equipment (1) bottom outside equidistance installs rubber seal ring (32) additional, seal groove (33) with rubber seal ring (32) looks adaptation are seted up to adapter sleeve (3) inner wall, and rubber seal ring (32) embedding is in seal groove (33) inner seal connection.
4. The device for sampling immune cells of claim 1, wherein: an auxiliary sheet (52) is additionally arranged at the top end of the grid separation sheet (5), and the auxiliary sheet (52) is connected with the grid separation sheet (5) through a connecting column (51).
5. The device of claim 4, wherein: the auxiliary sheet (52) is in accordance with the specification of the grid baffle sheet (5).
6. The device of claim 5, wherein: the top end of the auxiliary sheet (52) is additionally provided with a silica gel ring (53), the bottom end of the baffle ring (43) is provided with a corresponding sealing cavity (54), and the silica gel ring (53) is embedded in the sealing cavity (54) and connected in a sealing manner.
7. The device of claim 6, wherein: the inner top end and the outer top end of the silica gel ring (53) are provided with inclined planes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011345695.9A CN112481103B (en) | 2020-11-26 | 2020-11-26 | High-efficient immune cell sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011345695.9A CN112481103B (en) | 2020-11-26 | 2020-11-26 | High-efficient immune cell sampling device |
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| Publication Number | Publication Date |
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| CN112481103A true CN112481103A (en) | 2021-03-12 |
| CN112481103B CN112481103B (en) | 2023-11-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011345695.9A Active CN112481103B (en) | 2020-11-26 | 2020-11-26 | High-efficient immune cell sampling device |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201814582U (en) * | 2010-10-25 | 2011-05-04 | 孙玉芹 | Anti-pollution check blood taking needle |
| US20120129164A1 (en) * | 2009-11-04 | 2012-05-24 | Yvon Cayre | Device and process for isolating and cultivating live cells on a filter or extracting their genetic material |
| CN104758008A (en) * | 2014-01-03 | 2015-07-08 | 黄海涛 | Biological tissue puncture device and biological tissue puncture method |
| CN204542231U (en) * | 2015-04-13 | 2015-08-12 | 李俊华 | A kind of cell sample sampler |
| CN210736761U (en) * | 2019-09-11 | 2020-06-12 | 山西医科大学汾阳学院 | Immune cell sampling device |
| CN211862863U (en) * | 2019-11-29 | 2020-11-06 | 宋晓霞 | Prevent blockking up tumour biopsy sampler |
-
2020
- 2020-11-26 CN CN202011345695.9A patent/CN112481103B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120129164A1 (en) * | 2009-11-04 | 2012-05-24 | Yvon Cayre | Device and process for isolating and cultivating live cells on a filter or extracting their genetic material |
| CN201814582U (en) * | 2010-10-25 | 2011-05-04 | 孙玉芹 | Anti-pollution check blood taking needle |
| CN104758008A (en) * | 2014-01-03 | 2015-07-08 | 黄海涛 | Biological tissue puncture device and biological tissue puncture method |
| CN204542231U (en) * | 2015-04-13 | 2015-08-12 | 李俊华 | A kind of cell sample sampler |
| CN210736761U (en) * | 2019-09-11 | 2020-06-12 | 山西医科大学汾阳学院 | Immune cell sampling device |
| CN211862863U (en) * | 2019-11-29 | 2020-11-06 | 宋晓霞 | Prevent blockking up tumour biopsy sampler |
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| CN112481103B (en) | 2023-11-28 |
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