CN112457993B - Nutritive salt for promoting quick high-density growth of Alternaria hainanensis and culture method - Google Patents
Nutritive salt for promoting quick high-density growth of Alternaria hainanensis and culture method Download PDFInfo
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Abstract
The invention discloses nutritive salt for promoting the quick high-density growth of Alternaria hainanensis and a culture method, and relates to the technical field of aquaculture. The nutrient salt comprises the following components: ferric citrate, sodium silicate, monopotassium phosphate, urea and disodium ethylene diamine tetraacetate. According to the invention, the urea is added as a part of the nutrient salt, so that the dosage of other components in the nutrient salt can be reduced, the overall cost of the nutrient salt is reduced due to the lower cost of the urea, the culture cost of the Alternaria hainanensis is reduced, and the rapid high-density growth of the Alternaria hainanensis is promoted by the vitamin C and the carbon dioxide in the air.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to a nutritive salt for promoting the quick high-density growth of Alternaria hainanensis and a culture method.
Background
Alternaria (Thalassiras) belongs to the phylum Diatom (Bacillariophyta), the order Diatoma (Centrales), the order Diatoma (Discoideae), the family Thalassiaceae (Thalassiceae) and the genus Thalassia (Thalassira), and the colloid silk is mostly connected to form chain-shaped groups (4-12 cells) at the edges of the shell surface, and the single cell has the length of the axis of passing through the shell of 20-40 microns and the diameter of 5-15 microns. The bait has the characteristics of high nutritive value, strong adaptability, easy culture and the like, and thus becomes an excellent initial feeding for the seawater crustacean fries. At present, the nutrient salt for culturing the hainanensis is complex in components and high in cost, and a laboratory culture method is complex and is not suitable for open pond culture conditions of the hainanensis.
Disclosure of Invention
The invention aims to provide a nutrient salt for promoting the rapid and high-density growth of the Alhaica hainanensis, so as to solve one or more technical problems in the prior art and provide at least one beneficial selection or creation condition.
The technical scheme adopted for solving the technical problems is as follows:
a nutritive salt for promoting the quick and high-density growth of Alternaria hainanensis comprises the following components: ferric citrate, sodium silicate, monopotassium phosphate, urea and disodium ethylene diamine tetraacetate.
Preferably, the nutrient salt comprises the following components in g per ton of seawater: 1.5-2 g/ton ferric citrate, 20-30 g/ton sodium silicate, 2-3 g/ton potassium dihydrogen phosphate, 40-50 g/ton urea and 20-30 g/ton disodium ethylenediamine tetraacetate.
The invention also aims to provide a method for culturing the Alhaica hainanensis by using the nutrient salt for promoting the Alhainanensis to grow rapidly and at high density, which comprises the following steps:
s1, sequentially precipitating impurities in seawater through a sedimentation tank, filtering the seawater through a sand filter, then adding the seawater into a reservoir, adding 2-5 g of bleaching powder into each ton of seawater to sterilize and kill protozoon, and aerating for 24-48 hours for later use;
s2, pumping the seawater in the reservoir into a culture pond, adding 0.8-1.5 g of sodium thiosulfate into each ton of seawater for dechlorination, uniformly stirring and dissolving the sodium thiosulfate by using a proper amount of water, then splashing the sodium thiosulfate into the culture pond, wherein the addition amount of the sodium thiosulfate is determined by the chloride ion content in the seawater and is added from a small amount to a large amount, the chloride ion content can be detected and observed by a residual chloride detection kit, so that the final chloride ion content in the seawater is ensured to be below 0.025mg/L, introducing air for 3-8 min, and then adding 0.8-1.5 g of vitamin C into each ton of seawater;
s3, according to the g number of the components contained in each ton of seawater, 1.5-2 g/ton of ferric citrate is uniformly stirred and dissolved by using a proper amount of hot water, and then is splashed into a culture pond, after 20-30 g/ton of sodium silicate, 2-3 g/ton of potassium dihydrogen phosphate and 40-50 g/ton of urea are uniformly stirred and dissolved by using a proper amount of water, the ferric citrate is splashed into the culture pond, after 20-30 g/ton of disodium ethylene diamine tetraacetate is uniformly stirred and dissolved by using a proper amount of water, the disodium ethylene diamine tetraacetate is splashed into the culture pond, and then air is introduced for 3-8 min; the carbon dioxide contained in the air is beneficial to the photosynthesis of the hainanensis; the nutritive salt is fully dissolved before being added into the seawater, thereby being beneficial to the absorption of the Alternaria hainanensis, promoting the good metabolism of the Alternaria hainanensis and obtaining good growth.
S4, inoculating 1-2L of the algae of the Alhaica hainanensis into 10-12L of seawater in the culture pond, and culturing in an environment with the temperature of 25-32 ℃ and the illumination intensity of 4000-8000 Lux.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the urea is added as a part of the nutrient salt, so that the dosage of other components in the nutrient salt can be reduced, and the overall cost of the nutrient salt is reduced due to the lower cost of the urea, thereby reducing the culture cost of the Alternaria hainanensis; according to the invention, ferric citrate is dissolved in hot water, and is easily dissolved in the hot water, and is gradually reduced into ferrous salt under the influence of heat, and some ferric ions which are not reduced are also provided, the ferric ions can be completely converted into ferrous ions by adding vitamin C, the ferrous ions can promote photosynthesis, and simultaneously air introduced into seawater is matched, the air contains carbon dioxide, so that the photosynthesis effect is further enhanced, and the rapid high-density growth of the sea chain algae is promoted; the method for culturing the hainanensis is simple and easy to implement and is suitable for large-scale open pond culture of the hainanensis.
Detailed Description
The concept and technical effects of the present invention will be clearly and completely described below in conjunction with the embodiments to fully understand the objects, features and effects of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and those skilled in the art can obtain other embodiments without inventive effort based on the embodiments of the present invention, and all embodiments are within the protection scope of the present invention.
Example 1
The nutrient salt for promoting the quick and high-density growth of the Alhaica hainanensis comprises the following components in g per ton of seawater: 1.5 g/ton of ferric citrate, 20 g/ton of sodium silicate, 2 g/ton of monopotassium phosphate, 40 g/ton of urea and 20 g/ton of disodium ethylene diamine tetraacetate.
The method for culturing the Alternaria hainanensis by utilizing the nutrient salt for promoting the Alternaria hainanensis to grow fast and at high density comprises the following steps:
s1, sequentially precipitating impurities in seawater through a sedimentation tank, filtering the seawater through a sand filter, then adding the seawater into a reservoir, adding 2g of bleaching powder into each ton of seawater to sterilize and kill protozoon, and aerating for 24 hours for later use;
s2, pumping the seawater in the reservoir into a culture pond, adding 0.8g of sodium thiosulfate into each ton of seawater for dechlorination, uniformly stirring and dissolving the sodium thiosulfate by using a proper amount of water, and then splashing the sodium thiosulfate into the culture pond, wherein the adding amount of the sodium thiosulfate is determined by the chloride ion content in the seawater and is added from small to large, the chloride ion content can be detected and observed by a residual chloride detection kit, so that the final chloride ion content in the seawater is below 0.025mg/L, introducing air for 3min, and then adding 0.8g of vitamin C into each ton of seawater;
s3, according to the g number of the components contained in each ton of seawater, 1.5 g/ton of ferric citrate is uniformly stirred and dissolved by using a proper amount of hot water, and then is splashed into a culture pond, 20 g/ton of sodium silicate, 2 g/ton of potassium dihydrogen phosphate and 40 g/ton of urea are uniformly stirred and dissolved by using a proper amount of water, and then are splashed into the culture pond, 20 g/ton of disodium edetate is uniformly stirred and dissolved by using a proper amount of water, and then is splashed into the culture pond, and air is introduced for 3min;
and S4, inoculating 1L of the Alternaria hainanensis algae into 10L of seawater in the culture pond, and culturing at the temperature of 25 ℃ under the illumination intensity of 4000 Lux.
Example 2
The nutrient salt for promoting the quick and high-density growth of the Alhaica hainanensis comprises the following components in g per ton of seawater: 2 g/ton of ferric citrate, 30 g/ton of sodium silicate, 3 g/ton of monopotassium phosphate, 50 g/ton of urea and 30 g/ton of disodium ethylene diamine tetraacetate.
The method for culturing the Alternaria hainanensis by utilizing the nutrient salt for promoting the Alternaria hainanensis to grow fast and at high density comprises the following steps:
s1, sequentially passing seawater through a sedimentation tank to precipitate impurities, filtering the seawater through a sand filter, then entering a reservoir, adding 5g of bleaching powder into each ton of seawater to sterilize and kill original organisms, and aerating for 48 hours for later use;
s2, pumping the seawater in the reservoir into a culture pond, adding 1.5g of sodium thiosulfate into each ton of seawater for dechlorination, uniformly stirring and dissolving the sodium thiosulfate by using a proper amount of water, and then splashing the sodium thiosulfate into the culture pond, wherein the adding amount of the sodium thiosulfate is determined by the chloride ion content in the seawater and is added from small to large, the chloride ion content can be detected and observed by a residual chloride detection kit, so that the final chloride ion content in the seawater is below 0.025mg/L, introducing air for 8min, and adding 1.5g of vitamin C into each ton of seawater;
s3, according to the g number of the components contained in each ton of seawater, 2 g/ton of ferric citrate is uniformly stirred and dissolved by using a proper amount of hot water, and then is splashed into a culture pond, 30 g/ton of sodium silicate, 3 g/ton of potassium dihydrogen phosphate and 50 g/ton of urea are uniformly stirred and dissolved by using a proper amount of water, and then are splashed into the culture pond, 30 g/ton of disodium ethylene diamine tetraacetate is uniformly stirred and dissolved by using a proper amount of water, and then are splashed into the culture pond, and then air is introduced for 8min;
and S4, inoculating 2L of the Alternaria hainanensis algae into 12L of seawater in the culture pond, and culturing at the temperature of 32 ℃ under the illumination intensity of 8000 Lux.
Example 3
The nutrient salt for promoting the rapid high-density growth of the Alhaica hainanensis comprises the following components in g per ton of seawater: 1.8 g/ton of ferric citrate, 25 g/ton of sodium silicate, 2.5 g/ton of potassium dihydrogen phosphate, 45 g/ton of urea and 25 g/ton of disodium ethylene diamine tetraacetate.
The method for culturing the Alternaria hainanensis by utilizing the nutrient salt for promoting the Alternaria hainanensis to grow fast and at high density comprises the following steps:
s1, sequentially passing seawater through a sedimentation tank to precipitate impurities, filtering the seawater through a sand filter, then entering a reservoir, adding 3.5g of bleaching powder into each ton of seawater to sterilize and kill original organisms, and aerating for 36 hours for later use;
s2, pumping the seawater in the reservoir into a culture pond, adding 1.2g of sodium thiosulfate into each ton of seawater for dechlorination, uniformly stirring and dissolving the sodium thiosulfate by using a proper amount of water, then splashing the sodium thiosulfate into the culture pond, wherein the adding amount of the sodium thiosulfate is determined by the chloride ion content in the seawater and is added from small to large, the chloride ion content can be detected and observed by a residual chloride detection kit, the final chloride ion content in the seawater is ensured to be below 0.025mg/L, introducing air for 5min, and then adding 1.2g of vitamin C into each ton of seawater;
s3, according to the g number of the components contained in each ton of seawater, 1.8 g/ton of ferric citrate is uniformly stirred and dissolved by using a proper amount of hot water, and then is splashed into a culture pond, 25 g/ton of sodium silicate, 2.5 g/ton of potassium dihydrogen phosphate and 45 g/ton of urea are uniformly stirred and dissolved by using a proper amount of water, and then are splashed into the culture pond, 25 g/ton of disodium ethylene diamine tetraacetate is uniformly stirred and dissolved by using a proper amount of water, and then is splashed into the culture pond, and air is introduced for 5min;
and S4, inoculating 1L of the Alternaria hainanensis algae into every 11L of seawater in the culture pond, and culturing at the temperature of 28 ℃ under the illumination intensity of 6000 Lux.
Comparative example 1
The difference from example 1 is that no air is introduced into the seawater.
The nutrient salt for promoting the quick and high-density growth of the Alhaica hainanensis comprises the following components in g per ton of seawater: 1.5 g/ton of ferric citrate, 20 g/ton of sodium silicate, 2 g/ton of potassium dihydrogen phosphate, 40 g/ton of urea and 20 g/ton of disodium ethylene diamine tetraacetate.
The method for culturing the Alternaria hainanensis by utilizing the nutrient salt for promoting the Alternaria hainanensis to grow fast and at high density comprises the following steps:
s1, sequentially passing seawater through a sedimentation tank to precipitate impurities, filtering the seawater through a sand filter, then entering a reservoir, adding 2g of bleaching powder into each ton of seawater to sterilize and kill original organisms, and aerating for 24 hours for later use;
s2, pumping the seawater in the reservoir into a culture pond, adding 0.8g of sodium thiosulfate into each ton of seawater for dechlorination, uniformly stirring and dissolving the sodium thiosulfate by using a proper amount of water, and sprinkling the sodium thiosulfate into the culture pond, wherein the adding amount of the sodium thiosulfate is determined by the chloride ion content in the seawater and is added from small to large, the chloride ion content can be detected and observed by a residual chloride detection kit, so that the final chloride ion content in the seawater is ensured to be below 0.025mg/L, and then adding 0.8g of vitamin C into each ton of seawater;
s3, according to the g number of the components contained in each ton of seawater, 1.5 g/ton of ferric citrate is uniformly stirred and dissolved by using a proper amount of hot water, and then is splashed into a culture pond, 20 g/ton of sodium silicate, 2 g/ton of potassium dihydrogen phosphate and 40 g/ton of urea are uniformly stirred and dissolved by using a proper amount of water, and then are splashed into the culture pond, and 20 g/ton of disodium ethylenediamine tetraacetate is uniformly stirred and dissolved by using a proper amount of water, and then is splashed into the culture pond;
and S4, inoculating 1L of the Alternaria hainanensis algae into 10L of seawater in the culture pond, and culturing in an environment with the temperature of 25 ℃ and the illumination intensity of 4000 Lux.
Comparative example 2
The difference from example 1 is that no vitamin C is added to the seawater.
The nutrient salt for promoting the quick and high-density growth of the Alhaica hainanensis comprises the following components in g per ton of seawater: 1.5 g/ton of ferric citrate, 20 g/ton of sodium silicate, 2 g/ton of potassium dihydrogen phosphate, 40 g/ton of urea and 20 g/ton of disodium ethylene diamine tetraacetate.
The method for culturing the Alternaria hainanensis by using the nutritive salt for promoting the Alternaria hainanensis to grow fast and at high density comprises the following steps:
s1, sequentially precipitating impurities in seawater through a sedimentation tank, filtering the seawater through a sand filter, then adding the seawater into a reservoir, adding 2g of bleaching powder into each ton of seawater to sterilize and kill protozoon, and aerating for 24 hours for later use;
s2, pumping the seawater in the reservoir into a culture pond, adding 0.8g of sodium thiosulfate into each ton of seawater for dechlorination, uniformly stirring and dissolving the sodium thiosulfate in a proper amount of water, and then splashing the sodium thiosulfate into the culture pond, wherein the adding amount of the sodium thiosulfate is determined by the chloride ion content in the seawater and is added from a small amount to a large amount, the chloride ion content can be detected and observed by a residual chloride detection kit, so that the final chloride ion content in the seawater is ensured to be below 0.025mg/L, and introducing air for 3min;
s3, according to the g number of the components contained in each ton of seawater, 1.5 g/ton of ferric citrate is uniformly stirred and dissolved by using a proper amount of hot water, and then is splashed into a culture pond, 20 g/ton of sodium silicate, 2 g/ton of potassium dihydrogen phosphate and 40 g/ton of urea are uniformly stirred and dissolved by using a proper amount of water, and then are splashed into the culture pond, 20 g/ton of disodium edetate is uniformly stirred and dissolved by using a proper amount of water, and then is splashed into the culture pond, and air is introduced for 3min;
and S4, inoculating 1L of the Alternaria hainanensis algae into 10L of seawater in the culture pond, and culturing at the temperature of 25 ℃ under the illumination intensity of 4000 Lux.
Respectively taking water samples of different growth periods after the culture ponds of examples 1-3 and comparative examples 1-2 are inoculated with the algae seeds, filtering the collected water samples by a screen, attaching the algae samples to the screen, and suspending the algae samples attached to the screen again in a concentration of 10 3 mol/L ofIn a beaker of EDTA solution, the cells of the Alternaria hainanensis population are completely dispersed into single cells by magnetic stirring, the completely dispersed Alternaria hainanensis single cell sample is sucked, the number of the Alternaria hainanensis cells is counted by adopting a blood counting plate, the number of the cells in each 25 lattices on the blood counting plate is counted, the unit is one, and the result is shown in Table 1.
TABLE 1 cell numbers of Alternaria hainanensis in examples 1 to 3 and comparative examples 1 to 2
As can be seen from the data in Table 1, the growth rate of the Alternaria hainanensis is higher in examples 1-3 of the invention, but is lower than that of the Alternaria hainanensis in comparative examples 1-2, which shows that the invention can completely convert ferric ions into ferrous ions through vitamin C, the ferrous ions can promote photosynthesis, and simultaneously, the photosynthesis effect is further enhanced by matching with carbon dioxide in the air, and the rapid high-density growth of the Alternaria hainanensis is promoted.
While the preferred embodiments of the present invention have been illustrated and described, it will be understood by those skilled in the art that the present invention is not limited to the details of the embodiments shown and described, but is capable of numerous equivalents and substitutions without departing from the spirit of the invention as set forth in the claims appended hereto.
Claims (3)
1. A method for culturing Alhaina algae is characterized by comprising the following steps:
s1, sequentially carrying out precipitation, filtration, sterilization and aeration on seawater for later use;
s2, dechlorinating the seawater treated in the S1 to ensure that the content of chloride ions in the seawater is below 0.025mg/L, introducing air, and then adding vitamin C;
s3, adding ferric citrate, sodium silicate, monopotassium phosphate, urea and disodium ethylene diamine tetraacetate in the nutrient salt into the seawater treated by the S2, and introducing air;
s4, inoculating the haichow algae into the seawater treated in the S3 for culture;
the nutrient salt comprises the following components in g per ton of seawater: 1.5-2 g/ton ferric citrate, 20-30 g/ton sodium silicate, 2-3 g/ton potassium dihydrogen phosphate, 40-50 g/ton urea and 20-30 g/ton disodium ethylene diamine tetraacetate;
the addition amount of the vitamin C in the S2 is 0.8-1.5 g of the vitamin C added into each ton of seawater; in the step S3, ferric citrate is dissolved by hot water and then added into the seawater treated by the step S2; the step of inoculating the Alternaria hainanensis in the step S4 is to inoculate 1-2L of Alternaria hainanensis seeds in 10-12L of seawater;
the culture conditions in S4 are as follows: culturing at 25-32 deg.c and illumination intensity of 4000-8000 Lux.
2. The method according to claim 1, wherein the S1 sterilization is performed by adding 2-5 g of bleaching powder per ton of seawater.
3. The method of claim 1, wherein the chlorine removal in S2 is performed by adding 0.8-1.5 g sodium thiosulfate per ton seawater.
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