CN105199959A - Macro-element nutrient salt formula for large-scale culture of Thalassiossira sp. - Google Patents
Macro-element nutrient salt formula for large-scale culture of Thalassiossira sp. Download PDFInfo
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- CN105199959A CN105199959A CN201510686939.2A CN201510686939A CN105199959A CN 105199959 A CN105199959 A CN 105199959A CN 201510686939 A CN201510686939 A CN 201510686939A CN 105199959 A CN105199959 A CN 105199959A
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- seawater
- salt formula
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- thalassiossira
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Abstract
The invention discloses a macro-element nutrient salt formula for large-scale culture of Thalassiossira sp. The nutrient salt formula comprises the following components in content by gram per m<3> of seawater: 55 to 65g/m<3> of NaNO3, 75 to 86g/m<3> of NH4HCO3, 7 to 12 g/m<3> of Na2HPO4, 2.2 to 3.0g.m<3> of FeCl3, 9.0 to 10.5g/m<3> of EDTA-2Na and 16 to 24g/m<3> of NaSiO3. The macro-element nutrient salt formula for large-scale culture of Thalassiossira sp. has the advantages that the growth of Thalassiossira sp. can be promoted, the culture density is improved, the culture efficiency is improved and the cell nutrient components of the obtained Thalassiossira sp. are stable, further has the characteristics of high culture density, long duration and low cost, and compared with the prior art, can obviously improve the cell density of the Thalassiossira sp. and obviously prolong the culture time of the Thalassiossira sp.
Description
Technical field
The invention belongs to aquaculture field, especially a kind of macro-element nutrients salt formula being applicable to large scale culturing hailian seaweed.
Background technology
Micro-algae have also been obtained increasing concern as reproducible biomass energy; the industrialization of pilot scale culture micro-algae also has relevant research and obtains certain progress; but because the micro-algae of pilot scale culture is easily subject to the impact of the factor such as illumination, culture condition, still there is the deficiency that efficiency is low, cost is high in existing micro-algae industrialization mode.
Hailian seaweed (
thalassiossirasp.) belong to Bacillariophyta (
bacillariophyta), center of circle diatom order (
centrales), plate-like diatom suborder (
discoideae), hailian seaweed section (
thalassiosoraceae), Thalassiosira (
thalassiossira), become catenoid colony (4 ~ 12 cells) mainly with glial filament at shell face edge conjunction, individual cells pervalvar axis length is at 20-40 micron, and diameter is between 5-15 micron.Be easy to the features such as cultivation because it has very high nutritive value and strong adaptability and become the excellent open-mouthed bait of seawater crustaceans seed, but be main mainly with the mode of natural rich water, little purifying is cultivated.
Current laboratory and the F/2 substratum producing the many employings of upper cultivation hailian seaweed basic are cultivated, and F/2 substratum is mainly by NaNO
3, NaH
2pO
4h
2o, Na
2siO
39H
2o, Na
2eDTA, FeCl
36H
2o, CuSO
45H
2o, ZnSO
47H
2o, CoCl
26H
2o, MnCl4H
2o, Na
2moO
42H
2o, VITMAIN B1, vitamin B12, vitamin H, nature seawater form.F/2 substratum also exists the shortcomings such as vulnerable to pollution, culture density are low, and easily cause culture efficiency low, institute's cultured cells nutritive ingredient is unstable, has a strong impact on nursery process and Quality of Seedlings.
It is not enough that existing technology fails to solve this in hailian seaweed culturing process.
Summary of the invention
The object of the invention is the present situation of cultivating for current hailian seaweed; the nutritive salt formula of the macroelement of an applicable hailian seaweed large scale culturing is provided; nutritive salt of the present invention is used for the pilot scale culture of hailian seaweed; the growth of hailian seaweed can be promoted, improve culture density, improve culture efficiency; the hailian seaweed cytotrophy stable components obtained, also has and cultivates the feature that density is high, the cultivation time length is long, reduction aquaculture cost is low.
Technical scheme of the present invention is as follows: a kind of macro-element nutrients salt formula being applicable to large scale culturing hailian seaweed, wherein each component and content thereof are with every m
3containing this component g number meter in seawater, as follows:: NaNO
355 ~ 65g/m
3, NH
4hCO
375 ~ 86g/m
3, Na
2hPO
47 ~ 12g/m
3, FeCl
32.2 ~ 3.0g/m
3, EDTA-2Na9.0 ~ 10.5g/m
3, NaSiO
316 ~ 24g/m
3.
Preferred formula composition can be: this nutritive salt formula is with every m
3containing this component g number meter in seawater, as follows: NaNO
360g/m
3, NH
4hCO
380g/m
3, Na
2hPO
410g/m
3, FeCl
32.5g/m
3, EDTA-2Na9.5g/m
3, NaSiO
320g/m
3.
Preferred formula composition can also be: this nutritive salt formula is with every m
3containing this component g number meter in seawater, as follows: NaNO
358g/m
3, NH
4hCO
378g/m
3, Na
2hPO
411g/m
3, FeCl
32.8g/m
3, EDTA-2Na10g/m
3, NaSiO
322g/m
3.
Preferred formula composition can also be: this nutritive salt formula is with every m
3containing this component g number meter in seawater, as follows: NaNO
362g/m
3, NH
4hCO
383g/m
3, Na
2hPO
49g/m
3, FeCl
32.6g/m
3, EDTA-2Na9.8g/m
3, NaSiO
318g/m
3.
The large-scale cultivation method that nutritive salt formula of the present invention is used for hailian seaweed comprises the steps:
(1) be 200m by the area being used for large scale culturing hailian seaweed
2racetrack bio-reactor is cleared up thoroughly and is sterilized, then the seawater through sand filtration, foam separation, accurate filter and disinfection by ultraviolet light is introduced, the seawater depth of water 25 ~ 30cm, then according to the dosage of 20ppm, strong chlorine oil is added in seawater, carry out disinfection and aeration diel;
(2) seawater is after step (1) process, adds Sulfothiorine according to the dosage of 30ppm;
(3) fill a prescription according to claim 1, by Marine water volume, weigh each component, each component is added in seawater, open wheel slurry stirring 5 ~ 10min, the qualified hailian seaweed algae kind of access through detecting, after inoculation, cell density reaches (1 ~ 1.2) × 10
5/ ml, inoculates complete, and use wheel slurry to stir, wheel slurry, by motor drive, passes into CO simultaneously
2with adjust ph.
Described stirring velocity is: 80r/min.
Described pH value is 8-8.2.
Described component adds before described component adds seawater, first dissolves fully with fresh water.
The present invention has following beneficial effect: the present invention can promote the growth of hailian seaweed, improves culture density, improve culture efficiency, the hailian seaweed cytotrophy stable components obtained, also have and cultivate the feature that density is high, the cultivation time length is long, reduction aquaculture cost is low, relative to prior art, the cell density of hailian seaweed can be significantly improved, obviously extend the incubation time of hailian seaweed; In culturing process, before nutritive salt being added seawater, abundant dissolved nutrients, is conducive to the absorption of micro-algae, promotes its good metabolism, obtain good growth.
Embodiment
Be described in further details the present invention below by embodiment, these embodiments are only used for the present invention is described, do not limit the scope of the invention.
In order to make the object, technical solutions and advantages of the present invention definitely, below the preferred embodiments of the present invention are described in detail.
Embodiment 1 adopts following steps to carry out the large scale culturing of hailian seaweed:
(1) will be used for area is 200m
2racetrack bio-reactor is cleared up thoroughly and is sterilized, then the seawater through sand filtration, foam separation, accurate filter and disinfection by ultraviolet light is introduced, seawater depth of water 25cm, then according to the dosage of 20ppm, strong chlorine oil is added in seawater, carry out disinfection and aeration diel;
(2) seawater is after step (1) process, adds Sulfothiorine according to the dosage of 30ppm;
(3) by following formula, according to Marine water volume, each component is weighed, with every m
3containing this component g number meter: NaNO in seawater
360g/m
3, NH
4hCO
380g/m
3, Na
2hPO
410g/m
3, FeCl
32.5g/m
3, EDTA-2Na9.5g/m
3, NaSiO
320g/m
3;
(4) add in seawater by each component, open wheel slurry and stir 5min with rotating speed 80r/min, weigh nutritive salt according to corresponding ratio, fresh water fully dissolves simultaneously, then the qualified hailian seaweed algae kind of access through detecting, and after inoculation, algae cell density is 1.0 × 10
5individual/ml, inoculates complete, and use wheel slurry to stir, rotating speed 80r/min, wheel slurry, by motor drive, passes into CO simultaneously
2to regulate seawater pH value 8.
Implementation result: the hailian seaweed cell density that this experiment method is cultivated is high, reaches 1.1 × 10
6individual/ml, incubation time continue for 5 days.
Embodiment 2 adopts following steps to carry out the large scale culturing of hailian seaweed:
(1) will be used for area is 200m
2racetrack bio-reactor is cleared up thoroughly and is sterilized, then the seawater through sand filtration, foam separation, accurate filter and disinfection by ultraviolet light is introduced, seawater depth of water 30cm, then according to the dosage of 20ppm, strong chlorine oil is added in seawater, carry out disinfection and aeration diel;
(2) seawater is after step (1) process, adds Sulfothiorine according to the dosage of 30ppm;
(3) by following formula, according to Marine water volume, each component is weighed, with every m
3containing this component g number meter: NaNO in seawater
358g/m
3, NH
4hCO
378g/m
3, Na
2hPO
411g/m
3, FeCl
32.8g/m
3, EDTA-2Na10g/m
3, NaSiO
322g/m
3;
(4) add in seawater by each component, open wheel slurry and stir 10min with rotating speed 80r/min, weigh nutritive salt according to corresponding ratio, fresh water fully dissolves simultaneously, then the qualified hailian seaweed algae kind of access through detecting, and after inoculation, algae cell density is 1.1 × 10
5individual/ml, inoculates complete, and use wheel slurry to stir, rotating speed 80r/min, wheel slurry, by motor drive, passes into CO simultaneously
2to regulate seawater pH value 8.
Implementation result: the hailian seaweed cell density that this experiment method is cultivated is high, reaches 1.5 × 10
6individual/ml, incubation time continue for 6 days.
Embodiment 3 adopts following steps to carry out the large scale culturing of hailian seaweed:
(1) will be used for area is 200m
2racetrack bio-reactor is cleared up thoroughly and is sterilized, then the seawater through sand filtration, foam separation, accurate filter and disinfection by ultraviolet light is introduced, seawater depth of water 25cm, then according to the dosage of 20ppm, strong chlorine oil is added in seawater, carry out disinfection and aeration diel;
(2) seawater is after step (1) process, adds Sulfothiorine according to the dosage of 30ppm;
(3) by following formula, according to Marine water volume, each component is weighed, with every m
3containing this component g number meter: NaNO in seawater
362g/m
3, NH
4hCO
383g/m
3, Na
2hPO
49g/m
3, FeCl
32.6g/m
3, EDTA-2Na9.8g/m
3, NaSiO
318g/m
3;
(4) add in seawater by each component, open wheel slurry and stir with rotating speed 80r/min, weigh nutritive salt according to corresponding ratio, fresh water fully dissolves simultaneously, then the qualified hailian seaweed algae kind of access through detecting, and after inoculation, algae cell density is 1.2 × 10
5individual/ml, inoculates complete, and use wheel slurry to stir, rotating speed 80r/min, wheel slurry, by motor drive, passes into CO simultaneously
2to regulate seawater pH value 8.
Implementation result: the hailian seaweed cell density that this experiment method is cultivated is high, reaches 2.5 × 10
6individual/ml, incubation time continue for 7 days.
The above, it is only preferred embodiment of the present invention, not any pro forma restriction is done to the present invention, therefore everyly do not depart from technical solution of the present invention content, the any simple modification done above embodiment according to technical spirit of the present invention, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (8)
1. be applicable to a macro-element nutrients salt formula for large scale culturing hailian seaweed, it is characterized in that: in this nutritive salt formula, each component and content thereof are with every m
3containing this component g number meter in seawater, as follows: NaNO
355 ~ 65g/m
3, NH
4hCO
375 ~ 86g/m
3, Na
2hPO
47 ~ 12g/m
3, FeCl
32.2 ~ 3.0g/m
3, EDTA-2Na9.0 ~ 10.5g/m
3, NaSiO
316 ~ 24g/m
3.
2. macro-element nutrients salt formula according to claim 1, is characterized in that: this nutritive salt formula is with every m
3containing this component g number meter in seawater, as follows: NaNO
360g/m
3, NH
4hCO
380g/m
3, Na
2hPO
410g/m
3, FeCl
32.5g/m
3, EDTA-2Na9.5g/m
3, NaSiO
320g/m
3.
3. macro-element nutrients salt formula according to claim 1, is characterized in that: this nutritive salt formula is with every m
3containing this component g number meter in seawater, as follows: NaNO
358g/m
3, NH
4hCO
378g/m
3, Na
2hPO
411g/m
3, FeCl
32.8g/m
3, EDTA-2Na10g/m
3, NaSiO
322g/m
3.
4. macro-element nutrients salt formula according to claim 1, is characterized in that: this nutritive salt formula is with every m
3containing this component g number meter in seawater, as follows: NaNO
362g/m
3, NH
4hCO
383g/m
3, Na
2hPO
49g/m
3, FeCl
32.6g/m
3, EDTA-2Na9.8g/m
3, NaSiO
318g/m
3.
5. macro-element nutrients salt formula according to claim 1, is characterized in that: the large-scale cultivation method that this nutritive salt formula is used for hailian seaweed adopts following steps:
(1) be 200m by the area being used for large scale culturing hailian seaweed
2racetrack bio-reactor is cleared up thoroughly and is sterilized, then the seawater through sand filtration, foam separation, accurate filter and disinfection by ultraviolet light is introduced, the seawater depth of water 25 ~ 35cm, then according to the dosage of 20ppm, strong chlorine oil is added in seawater, carry out disinfection and aeration diel;
(2) seawater is after step (1) process, adds Sulfothiorine according to the dosage of 30ppm;
(3) fill a prescription according to claim 1, by Marine water volume, weigh each component, each component is added in seawater, open wheel slurry stirring 5 ~ 10min, the then qualified hailian seaweed algae kind of access through detecting, after making inoculation, cell density reaches (1.0 ~ 1.2) × 10
5/ ml, inoculates complete, and use wheel slurry to stir, wheel slurry, by motor drive, passes into CO simultaneously
2with adjust ph.
6. macro-element nutrients salt formula according to claim 5, is characterized in that: described stirring velocity is 80r/min.
7. macro-element nutrients salt formula according to claim 5, is characterized in that: described pH value is 8-8.2.
8. macro-element nutrients salt formula according to claim 5, is characterized in that: before described component adds seawater, first dissolves fully with fresh water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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|---|---|---|---|
| CN201510686939.2A CN105199959A (en) | 2015-10-22 | 2015-10-22 | Macro-element nutrient salt formula for large-scale culture of Thalassiossira sp. |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111349567A (en) * | 2020-04-03 | 2020-06-30 | 湛江国联水产开发股份有限公司 | Heterotrophic fermentation method suitable for Alternaria hainanensis |
| CN112457993A (en) * | 2020-11-09 | 2021-03-09 | 佛山科学技术学院 | Nutritive salt for promoting quick high-density growth of Alternaria hainanensis and culture method |
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2015
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111349567A (en) * | 2020-04-03 | 2020-06-30 | 湛江国联水产开发股份有限公司 | Heterotrophic fermentation method suitable for Alternaria hainanensis |
| CN112457993A (en) * | 2020-11-09 | 2021-03-09 | 佛山科学技术学院 | Nutritive salt for promoting quick high-density growth of Alternaria hainanensis and culture method |
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Application publication date: 20151230 |
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