CN107868811A - The method of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin - Google Patents
The method of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin Download PDFInfo
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Abstract
A kind of method of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin, it is that fresh haematococcus pluvialis akinete (is referred to and just harvests undried processing, complete astaxanthin accumulation and simultaneously complete life cycle haematococcus pluvialis akinete in a dormant state) mixed with the fortification culture medium containing nitrogen source and vitamin after cultivate, induce akinete propagation, broken wall, discharge swarm cell；Centrifugation obtains zoospore；Zoospore is handled with organic solvent, extracts astaxanthin；Frond is centrifuged, obtains organic solvent；Finally by the organic solvent rotary evaporation in vacuo of acquisition, astaxanthin is obtained.The present invention utilizes the mitotic physiological property of haematococcus pluvialis intracellular, and orienting regulation and control haematococcus pluvialis by fortification concentrates progress division growth to realize breaking-wall cell, with organic solvent low power consuming, efficiently extracts frond astaxanthin, more economical, green, environmentally friendly.
Description
Technical field
The invention belongs to algae functional components to extract field, is related to a kind of auxotype orientation regulation and control haematococcus pluvialis heavy wall
The method of sporogony extraction astaxanthin.
Background technology
In recent years, organism of the haematococcus pluvialis as natural astaxanthin content highest (2.5-4.0% dry weights), it is cultivated
It is of great interest with the extraction of frond astaxanthin.Astaxanthin In Haematococcus Pluvialis is a kind of non-vitamin a originals keto-acid carotenoids
Element, its molecule contain hydroxyl and the beta-unsaturated ketone in conjugated double bond chain end, and wherein hydroxyl and ketone group forms hydroxy-ketone, this knot
Structure feature makes it have extremely strong inoxidizability.In addition, astaxanthin have enhancing immunity of organisms, can promote antibody produce and very
Strong colouring function.In recent years, it is numerous to have turned into domestic and international researcher and medicine, food, cosmetics, aquaculture etc. for astaxanthin
The study hotspot of industry, have broad application prospects and huge market potential.
Haematococcus pluvialis are a kind of fresh water monoplast green algas, have double flagellums, are under the jurisdiction of Chlorophyceae volvocales haematococcus section.
Its under unfavorable growing environment (such as intense light irradiation, nutritional deficiency, active oxygen mutagens) realize the accumulation of intracellular astaxanthin.Together
When, a series of change also occurs for cell physiological feature：Motionless akinete (red sporangiocyst body) is become by green swarm cell,
Cell becomes big, and cell membrane is thickening (including outermost three layers of sheath wall, the second layer and third layer cell membrane).Haematococcus pluvialis heavy wall
The distinctive three confluent monolayer cells wall construction of spore makes it, and broken wall is realized intracellular Astaxanthin extraction and utilized big as one in the industrial production
Technological difficulties.
Due to the thick cell membrane of haematococcus pluvialis akinete, and astaxanthin is to light, heat, the unstability of oxygen so that
Astaxanthin extraction complex process, cause the annual production of natural astaxanthin relatively low, it is expensive, and the broken wall of thick cell membrane is rain life
Haematococcus produces the committed step of astaxanthin.At present in industrial production, the wall-breaking method of haematococcus pluvialis akinete cell has
Machinery broken wall law, chemical method broken wall, enzymatic shell-broken etc..Machinery broken wall law refers to using the machinery such as colloid mill, grinding, high-pressure homogeneous
Broken wall is carried out, although broken wall efficiency high, up to more than 90%, power consumption is high, and production cost is high, makes natural shrimp green grass or young crops in the market
Element holds at high price, and life of the caused high temperature to the Astaxanthin In Haematococcus Pluvialis of thermal sensitivity during tradition machinery broken wall
Thing activity influence is very big, makes astaxanthin oxidation aggravation, influences product quality.Chemical method broken wall common acid, alkali heat-treatment method, it is broken
Wall efficiency can reach more than 80%, but because using strong acid and strong base during broken wall, the requirement to equipment is higher, and strong acid-base environment
To making astaxanthin degrade, bioactivity is lost；Meanwhile in production caused industrial highly acid or alkaline waste water improvement it is also very big
Increase production cost.Enzymatic shell-broken is mainly to utilize one or more of enzymes in lysozyme, lipase, cellulase, pectase
Synergy, the sporoderm-broken rate of the method is ineffective 60% or so, and the cost of enzyme and, consumption also higher to technological requirement
When it is longer, be unfavorable for mass producing.The method of haematococcus pluvialis extraction astaxanthin at present, because of respective deficiency, make life
Production cost remains high, so seeking green, environmentally friendly, efficient wall-breaking method, reduces Astaxanthin In Haematococcus Pluvialis production cost,
It is urgent problem to be solved in current production application.
Chinese invention patent CN104946532A discloses a kind of method of haematococcus pluvialis broken wall, this method first with
Milling treatment of colloid makes cell membrane thinning, then carries out broken wall treatment to haematococcus pluvialis using homogenizer, finally utilizes acetone pair
Astaxanthin In Haematococcus Pluvialis is extracted, and its yield reaches 99.3%.Chinese invention patent CN1786148A discloses a kind of micro-
The method of frustule broken wall, this method realize that microalgae cell broken wall and intracellular functional materials are quick using high-pressure homogeneous, efficiently
Extraction.Chinese invention patent CN104762211A discloses a kind of method of haematococcus pluvialis broken wall, and this method compares grinding
Method, high-pressure homogenization, high speed bead mill method carry out broken wall to haematococcus pluvialis akinete, extract frond astaxanthin efficiency, as a result
Show that high-pressure homogenization shell-broken effect is better than polishing and high speed bead mill method, and industrial requirement can be met.It is but above-mentioned
Several method substantially belongs to mechanical breaking-wall method.
The Reproduction methods of haematococcus pluvialis include a variety of sides such as division growth, budding propagation according to the difference of culture environment
Formula.Haematococcus pluvialis akinete division growth feature is that haematococcus pluvialis multiplicative stage akinete has first carried out silk into the cell
Division, 8 divisions and 16 and 32 divisions are common are, after the completion of mitosis, the thick cell membrane of akinete is as caused by itself
Oval swarm cell is presented with two flagellums for enzyme dissolving, rupture, release, and this process typically continues 1-2 days.Astaxanthin conduct
A kind of metabolic by-product in inverse ring border residing for haematococcus pluvialis, continue to accumulate within 2-3 hours in swarm cell, compared to
The cell membrane of akinete multi-layer thick, the swarm cell in this period only have the organic solvent being made up of cellulose and easily penetrated
Thin cell membrane.Therefore, the present invention orients using the characteristic of haematococcus pluvialis division growth by nutrition, promotes rain life red
Ball algae carry out intracellular mitosis propagation, shorten generation time, realize breaking-wall cell, meanwhile, organic solvent low temperature, low power consuming,
Efficiently to Astaxanthin extraction.
The content of the invention
The technical problems to be solved by the invention are：For above-mentioned the deficiencies in the prior art, there is provided a kind of auxotype orientation
Regulate and control the method for haematococcus pluvialis akinete propagation extraction astaxanthin, oriented using nitrogen source or vitamin enrichment culture medium
Regulation and control, promote haematococcus pluvialis akinete to carry out fission, realize that akinete broken wall, frond shrimp are blue or green by division growth
Element extraction.This method can extract haematococcus pluvialis frond astaxanthin at a lower temperature, realize the green of overall process, environmental protection,
Low energy consumption.
In order to solve the above technical problems, the technical solution adopted in the present invention is：A kind of auxotype orientation regulation and control rain life is red
The method that ball algae akinete breeds extraction astaxanthin, this method step are as follows：
(1) fresh haematococcus pluvialis akinete and the fortification culture medium containing nitrogen source and vitamin are pressed 1:2-1:
4 weight cultivates 7-10h, induction haematococcus pluvialis akinete carries out born of the same parents at room temperature than mixing with rotating speed 30-60r/min
Interior division growth, broken wall, discharge zoospore；
Above-mentioned fresh haematococcus pluvialis akinete refers to just harvest undried processing, and it is simultaneously complete to complete astaxanthin accumulation
Into the haematococcus pluvialis akinete of a life cycle in a dormant state；
(2) centrifuge, obtain zoospore；
(3) in 60-70 DEG C, zoospore and organic solvent are pressed 1:10-1:20 volume ratio mixing, with 30-60r/min
Extract 2-4h；
(4) frond is centrifuged, obtains organic solvent；The organic solvent rotary evaporation in vacuo obtained will be separated, recovery has
Solvent, obtain astaxanthin.
The composition of the above-mentioned fortification culture medium referred to is to add to have in every 0.3mlPIV liquid and 99.7ml deionized waters
Ca(NO3)2·4H2O 15-60mg、KNO310-40mg, sodium β-glycerophosphate five water 5mg, MgSO4·7H2O 5mg, vitamin
B120.01-0.04 μ g, biotin 0.01-0.04 μ g, vitamin B11-4 μ g and trihydroxy methyl first ammonia 50mg, regulation pH to
7.5；Wherein, the composition of PIV liquid is to have Na per addition in 100ml deionized waters2EDTA·2H2O 100mg、FeCl3·6H2O
19.6mg、MnCl2·4H2O 3.6mg、ZnCl2 1.04mg、CoCl2·6H2O 0.4mg and Na2MoO4·2H2O 0.2mg。
Centrifugal rotational speed in above-mentioned steps (2) is 1000-3000r/min.
Organic solvent in above-mentioned steps (3) is methanol, ethanol, acetone, ethyl acetate, chloroform or n-hexane.
Centrifugal rotational speed in above-mentioned steps (4) is 1000-3000r/min；The temperature of rotary evaporation in vacuo is 35-60 DEG C.
The present invention technical principle be：Haematococcus pluvialis complete intracellular astaxanthin accumulation under unfavorable growth conditions, enter
Enter akinete (non motile cell) dormant physiology state, complete a life cycle, multi-layer thick is thin possessed by akinete
Cell wall significantly limit the extraction and application of haematococcus pluvialis frond astaxanthin.Into dormant state akinete in suitable life
The various ways such as budding and mitosis will be taken to carry out propagation under elongate member and enter next life cycle.The present invention utilizes nitrogen
Source or vitamin enrichment culture medium orientation regulation and control haematococcus pluvialis carry out intracellular mitosis, and this fortification culture medium can be effective
Ground regulates and controls, and strengthens haematococcus pluvialis and concentrates progress intracellular mitosis, shorten the time of division growth, is cultivating 7-10 hour
Interior more than 95% frond completes mitosis, complete mitotic akinete in the short period of time (20-40min) its
Cell membrane will melt automatically, rupture release intracellular zoospore (swarm cell).Meanwhile astaxanthin is as residing for haematococcus pluvialis
The metabolic by-product of inverse environment generation will continue to accumulate in swarm cell within 2-3 hours.Compared to akinete multi-layer thick
Cell membrane, swarm cell only forms the cell membrane that thin organic solvent is easy to penetrate by cellulose, and therefore, the present invention utilizes
The mitotic physiological property of haematococcus pluvialis intracellular, regulation and control haematococcus pluvialis are oriented by fortification and concentrated into line splitting increasing
Grow and realize breaking-wall cell, with organic solvent low power consuming, efficiently extract frond astaxanthin.
Compared with prior art, the present invention has advantages below and beneficial effect：
1) present invention utilizes haematococcus pluvialis intracellular division growth characteristic broken wall, compared with traditional machinery broken wall law, energy
Consumption can be ignored, and not have particular/special requirement to equipment during broken wall, compared to the high energy consumption of mechanical breaking-wall method and high
Cost of equipment, this method are more economical, green, environmentally friendly.
2) this method is cultivated at room temperature, and Extracting temperature is also no more than during 70 DEG C, with tradition machinery broken wall
Caused high-temperature-phase pair, this method influence very little on astaxanthin bioactivity, and product quality is high.
3) present invention realizes that organic solvent effectively reclaims, during there is no any material to remain, accomplish extraction process green,
It is environmentally friendly, efficient.
Brief description of the drawings
Fig. 1 is frond physiological status figure of the haematococcus pluvialis in fortification culture medium under different incubation times；
Wherein：(a) 0h haematococcus pluvialis akinete is cultivated；(b) mitosis propagation rain at the initial stage life for cultivating 4h is red
Ball algae akinete；(c) cultivate 7h and complete mitosis propagation haematococcus pluvialis akinete；(d) haematococcus pluvialis heavy wall spore
Sub- broken wall discharges zoospore.Figure medium scale is 20 μm.
Fig. 2 is to Astaxanthin In Haematococcus Pluvialis without broken wall treatment and through ethanol under the conditions of mitosis propagation broken wall treatment
Recovery rate compare.
Embodiment
The present invention is further elaborated with reference to the embodiment by taking haematococcus pluvialis as an example, but the implementation of the present invention
Mode not limited to this, there can also be many deformations.
The method that the present invention breeds extraction astaxanthin for auxotype orientation regulation and control haematococcus pluvialis akinete, specifically
For：Fresh haematococcus pluvialis akinete is taken (just to harvest undried processing, completed astaxanthin accumulation and complete a life
The haematococcus pluvialis akinete of cycle in a dormant state), itself and fortification culture medium are pressed 1:2-1:4 weight is than mixed
It is placed in after conjunction in culture tank (FS-5, Jiangsu Shuo Yun landification equipments Co., Ltd), is cultivated at room temperature with rotating speed 30-60r/min
7-10h, induction haematococcus pluvialis akinete carry out intracellular division growth, broken wall, discharge zoospore；Then in horizontal type scraper
Centrifuged in conveyer centrifugal (WG-450 Shanghai chemical machinery Co., Ltd., Factory) with 1000-3000r/min, obtain zoospore；
The zoospore of acquisition and organic solvent are pressed 1:10-1:20 volume ratio mixing is placed in culture tank, and (FS-5, Jiangsu the first day of the lunar month weed petrochemical industry
Equipment Limited) in, 2-4h is extracted with 30-60r/min in 60-70 DEG C；Then at horizontal scraper discharging centrifuge (on WG-450
Extra large chemical machinery Co., Ltd., Factory) in 1000-3000r/min centrifuge frond, obtain organic solvent；Finally separation is obtained
The organic solvent obtained reclaims organic solvent, obtains astaxanthin in 35-60 DEG C of rotary evaporation in vacuo.
The composition of the above-mentioned fortification culture medium referred to is to add to have in every 0.3mlPIV liquid and 99.7ml deionized waters
Ca(NO3)2·4H2O 15-60mg、KNO310-40mg, sodium β-glycerophosphate five water 5mg, MgSO4·7H2O 5mg, vitamin
B120.01-0.04 μ g, biotin 0.01-0.04 μ g, vitamin B11-4 μ g and trihydroxy methyl first ammonia 50mg, regulation pH to
7.5；Wherein, the composition of PIV liquid is to have Na per addition in 100ml deionized waters2EDTA·2H2O 100mg、FeCl3·6H2O
19.6mg、MnCl2·4H2O 3.6mg、ZnCl2 1.04mg、CoCl2·6H2O 0.4mg and Na2MoO4·2H2O 0.2mg。
The above-mentioned organic solvent referred to is methanol, ethanol, acetone, ethyl acetate, chloroform or n-hexane.
Embodiment 1
Fortification culture medium：0.3mlPIV liquid, 99.7ml deionized waters, Ca (NO3)2·4H2O 45mg、KNO3
30mg, sodium β-glycerophosphate five water 5mg, MgSO4·7H2O 5mg, vitamin B120.02 μ g, μ g of biotin 0.02, dimension life
Plain B12 μ g and trihydroxy methyl first ammonia 50mg, adjust pH to 7.5；
The composition of PIV liquid：100ml deionized waters, Na2EDTA·2H2O 100mg、FeCl3·6H2O 19.6mg、
MnCl2·4H2O 3.6mg、ZnCl2 1.04mg、CoCl2·6H2O 0.4mg and Na2MoO4·2H2O 0.2mg。
Fresh haematococcus pluvialis akinete is taken (just to harvest undried processing, completed astaxanthin accumulation and completion one
The haematococcus pluvialis akinete of individual life cycle in a dormant state) 50kg (content astaxanthin is 3% dry weight meter), by its with
It is placed in after the mixing of 150kg fortifications culture medium in culture tank, 8h is cultivated with rotating speed 45r/min at room temperature, induction rain life is red
Ball algae akinete carries out intracellular division growth, broken wall, discharges zoospore；Then in horizontal scraper discharging centrifuge with
2000r/min is centrifuged, and obtains zoospore；The zoospore of acquisition and organic solvent ethanol are pressed 1:15 volume ratio mixing is put
In culture tank, 3h is extracted with 45r/min in 65 DEG C；Centrifuged in horizontal scraper discharging centrifuge with 2000r/min
Frond, obtain organic solvent；The organic solvent obtained will finally be separated in 45 DEG C of rotary evaporation in vacuo, ethanol is reclaimed, obtain shrimp
Blue or green element.Astaxanthin is dried in vacuo, weighs the quality of astaxanthin, obtaining Astaxanthin extraction rate, (astaxanthin quality/rain life is red for 2.57%
Ball algae quality (dry weight meter) * 100%), Astaxanthin In Haematococcus Pluvialis total amount 85.7% is accounted for, haematococcus pluvialis are without broken wall treatment profit
Recovery rate with ethanol extraction astaxanthin is 0.13%, Astaxanthin In Haematococcus Pluvialis total amount 4.3% is accounted for, with reference to referring to Fig. 2.
Embodiment 2
Fortification culture medium：0.3mlPIV liquid, 99.7ml deionized waters, Ca (NO3)2·4H2O mg、KNO3 10mg、
Sodium β-glycerophosphate five water 5mg, MgSO4·7H2O 5mg, vitamin B120.04 μ g, μ g of biotin 0.04, vitamin B1 4μ
G and trihydroxy methyl first ammonia 50mg, adjust pH to 7.5；
The composition of PIV liquid：100ml deionized waters, Na2EDTA·2H2O 100mg、FeCl3·6H2O 19.6mg、
MnCl2·4H2O 3.6mg、ZnCl2 1.04mg、CoCl2·6H2O 0.4mg and Na2MoO4·2H2O 0.2mg。
Fresh haematococcus pluvialis akinete is taken (just to harvest undried processing, completed astaxanthin accumulation and completion one
The haematococcus pluvialis akinete of individual life cycle in a dormant state) 75kg (content astaxanthin is 3% dry weight meter), by its with
It is placed in after the mixing of 150kg fortifications culture medium in culture tank, 10h is cultivated with rotating speed 30r/min at room temperature, induction rain life is red
Ball algae akinete carries out intracellular division growth, broken wall, discharges zoospore；Then in horizontal scraper discharging centrifuge with
1000r/min is centrifuged, and obtains zoospore；By the zoospore of acquisition and organic solvent methanol by volume 1:10 mixing are placed in
In culture tank, 2h is extracted with 60r/min in 60 DEG C；Algae is centrifuged with 1000r/min in horizontal scraper discharging centrifuge
Body, obtain organic solvent；The organic solvent obtained will finally be separated in 35 DEG C of rotary evaporation in vacuo, methanol is reclaimed, it is blue or green to obtain shrimp
Element.Astaxanthin is dried in vacuo, the quality for weighing astaxanthin obtains Astaxanthin extraction rate as the 2.41% (astaxanthin quality/red ball of rain life
Algae quality (dry weight meter) * 100%), Astaxanthin In Haematococcus Pluvialis total amount 80.3% is accounted for, haematococcus pluvialis utilize without broken wall treatment
The recovery rate of methanol extraction astaxanthin is 0.11%, accounts for Astaxanthin In Haematococcus Pluvialis total amount 3.7%.
Embodiment 3
Fortification culture medium：0.3mlPIV liquid, 99.7ml deionized waters, Ca (NO3)2·4H2O 60mg、KNO3
40mg, sodium β-glycerophosphate five water 5mg, MgSO4·7H2O 5mg, vitamin B120.01 μ g, μ g of biotin 0.01, dimension life
Plain B11 μ g and trihydroxy methyl first ammonia 50mg, adjust pH to 7.5；
The composition of PIV liquid：100ml deionized waters, Na2EDTA·2H2O 100mg、FeCl3·6H2O 19.6mg、
MnCl2·4H2O 3.6mg、ZnCl2 1.04mg、CoCl2·6H2O 0.4mg and Na2MoO4·2H2O 0.2mg。
Fresh haematococcus pluvialis akinete is taken (just to harvest undried processing, completed astaxanthin accumulation and completion one
The haematococcus pluvialis akinete of individual life cycle in a dormant state) 25kg (content astaxanthin is 3% dry weight meter), by its with
It is placed in after the mixing of 100kg fortifications culture medium in culture tank, 7h is cultivated with rotating speed 60r/min at room temperature, induction rain life is red
Ball algae akinete carries out intracellular division growth, broken wall, discharges zoospore；Then in horizontal scraper discharging centrifuge with
3000r/min is centrifuged, and obtains zoospore；By the zoospore of acquisition and organic solvent-acetone by volume 1:20 mixing are placed in
In culture tank, 4h is extracted with 30r/min in 70 DEG C；Algae is centrifuged with 3000r/min in horizontal scraper discharging centrifuge
Body, obtain organic solvent；The organic solvent obtained will finally be separated in 60 DEG C of rotary evaporation in vacuo, acetone is reclaimed, it is blue or green to obtain shrimp
Element.Astaxanthin is dried in vacuo, the quality for weighing astaxanthin obtains Astaxanthin extraction rate as the 2.29% (astaxanthin quality/red ball of rain life
Algae quality (dry weight meter) * 100%), Astaxanthin In Haematococcus Pluvialis total amount 76.3% is accounted for, haematococcus pluvialis utilize without broken wall treatment
The recovery rate of acetone extraction astaxanthin is 0.17%, accounts for Astaxanthin In Haematococcus Pluvialis total amount 5.7%.
Claims (6)
1. a kind of method of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin, its feature exist
In this method step is as follows：
(1) fresh haematococcus pluvialis akinete and the fortification culture medium containing nitrogen source and vitamin are pressed 1:2-1:4
Weight cultivates 7-10h, induction haematococcus pluvialis akinete carries out intracellular point at room temperature than mixing with rotating speed 30-60r/min
Propagation, broken wall are split, discharges zoospore；
Above-mentioned fresh haematococcus pluvialis akinete refers to just harvest undried processing, completes astaxanthin accumulation and completes one
The haematococcus pluvialis akinete of individual life cycle in a dormant state；
(2) centrifuge, obtain zoospore；
(3) in 60-70 DEG C, zoospore and organic solvent are pressed 1:10-1:20 volume ratio mixing, is extracted with 30-60r/min
2-4h；
(4) frond is centrifuged, obtains organic solvent；The organic solvent rotary evaporation in vacuo obtained will be separated, recovery is organic molten
Agent, obtain astaxanthin.
2. the side of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin as claimed in claim 1
Method, it is characterised in that the composition of the fortification culture medium is to add to have in every 0.3mlPIV liquid and 99.7ml deionized waters
Ca(NO3)2·4H2O 15-60mg、KNO310-40mg, sodium β-glycerophosphate five water 5mg, MgSO4·7H2O 5mg, vitamin
B120.01-0.04 μ g, biotin 0.01-0.04 μ g, vitamin B11-4 μ g and trihydroxy methyl first ammonia 50mg, regulation pH to
7.5；Wherein, the composition of PIV liquid is to have Na per addition in 100ml deionized waters2EDTA·2H2O 100mg、FeCl3·6H2O
19.6mg、MnCl2·4H2O 3.6mg、ZnCl2 1.04mg、CoCl2·6H2O 0.4mg and Na2MoO4·2H2O 0.2mg。
3. the side of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin as claimed in claim 1
Method, it is characterised in that the centrifugal rotational speed in the step (2) is 1000-3000r/min.
4. the side of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin as claimed in claim 1
Method, it is characterised in that the organic solvent in the step (3) is methanol, ethanol, acetone, ethyl acetate, chloroform or n-hexane.
5. the side of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin as claimed in claim 1
Method, it is characterised in that the centrifugal rotational speed in the step (4) is 1000-3000r/min.
6. the side of auxotype orientation regulation and control haematococcus pluvialis akinete propagation extraction astaxanthin as claimed in claim 1
Method, it is characterised in that the temperature of rotary evaporation in vacuo is 35-60 DEG C in the step (4).
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114230502A (en) * | 2021-12-30 | 2022-03-25 | 无锡江大百泰科技有限公司 | Astaxanthin extraction method |
| CN116622797A (en) * | 2023-04-03 | 2023-08-22 | 广州优卡思农业技术有限公司 | Method for extracting astaxanthin from haematococcus pluvialis |
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| CN105916993A (en) * | 2014-04-17 | 2016-08-31 | 高丽大学校产学协力团 | Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature |
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- 2017-11-13 CN CN201711111852.8A patent/CN107868811A/en active Pending
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114230502A (en) * | 2021-12-30 | 2022-03-25 | 无锡江大百泰科技有限公司 | Astaxanthin extraction method |
| CN114230502B (en) * | 2021-12-30 | 2023-09-01 | 无锡江大百泰科技有限公司 | Astaxanthin extraction method |
| CN116622797A (en) * | 2023-04-03 | 2023-08-22 | 广州优卡思农业技术有限公司 | Method for extracting astaxanthin from haematococcus pluvialis |
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Application publication date: 20180403 |