CN112444625A - Immunofluorescence detection system and control method thereof - Google Patents
Immunofluorescence detection system and control method thereof Download PDFInfo
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Abstract
The invention provides an immunofluorescence detection system, comprising: the main control module comprises a main communication interface; the driving module is connected with the main communication interface and comprises at least two driving interfaces and at least two optical coupling interfaces, wherein each driving interface is connected with at least two stepping motors, and each optical coupling interface is connected with an optical coupling; the main control module executes scheduling control according to a sample detection item selected by a user, and the driving module responds to the scheduling control and generates control instructions of corresponding stepping motors and the optical couplers according to the sample detection item selected by the user so as to control the corresponding stepping motors to execute corresponding operations and monitor the corresponding optical couplers, so that the sample detection item is carried out. The invention also provides a control method thereof. The invention has strong expansibility and can support a plurality of stepping motors and optical couplers. And upgrading and updating of different modules can be realized.
Description
Technical Field
The disclosed embodiments of the present invention relate to the field of optical detection diagnostic technology, and more particularly, to an immunofluorescence detection system and a control method of the immunofluorescence detection system.
Background
The immunoassay method is a method for detecting a trace amount of substance in a specimen by using an antigen-antibody reaction. Based on the specificity and sensitivity of antigen-antibody reaction, the immunodetection method is widely applied to a plurality of fields such as medical detection, biological detection and the like. Any substance can be detected by the immunodetection method as long as the corresponding specific antibody can be obtained.
Both the immuno-luminometers and flow cytometers work based on immunodetection methods. At present, the analysis or detection system based on the immunodetection method has the defects of single module function, poor expansibility and complicated upgrading and updating.
Disclosure of Invention
According to an embodiment of the present invention, an immunofluorescence detection system and a control method of immunofluorescence detection are provided to solve the above problems.
According to a first aspect of the present invention, an exemplary immunofluorescence detection system is disclosed, comprising: the main control module comprises a main communication interface; the driving module is connected with the main communication interface and comprises at least two driving interfaces and at least two optical coupling interfaces, wherein each driving interface is connected with at least two stepping motors, and each optical coupling interface is connected with an optical coupling; the main control module executes scheduling control according to a sample detection item selected by a user, and the driving module responds to the scheduling control and generates control instructions of corresponding stepping motors and the optical couplers according to the sample detection item selected by the user so as to control the corresponding stepping motors to execute corresponding operations and monitor the corresponding optical couplers, so that the sample detection item is carried out.
In some embodiments, the master control module further comprises: a first processor connected with the primary communication interface; the first field programmable gate array is connected with the first processor; and the multiple analog-to-digital conversion circuits are respectively connected with the first field programmable gate array, and each analog-to-digital conversion circuit is connected with one photoelectric sensor and is used for performing analog-to-digital conversion processing on the fluorescence signal acquired by the photoelectric sensor.
In some embodiments, the drive module further comprises: a slave communication interface connected with the master communication interface; the second microprocessor is connected with the slave communication interface; and the second field programmable gate array is connected with the second microprocessor, the at least two driving interfaces and the at least two optical coupling interfaces. In some embodiments, the at least two drive interfaces comprise six drive interfaces.
In some embodiments, each of the drive interfaces is connected to six stepper motors.
In some embodiments, further comprising: the reagent needle liquid level detection module is connected with the second field programmable gate array and used for realizing the reagent needle liquid level detection function when executing the sample detection items; the sampling needle liquid level detection module is connected with the second field programmable gate array and used for realizing the sampling needle liquid level detection function when executing a sample detection item; and the pressure detection module is connected with the second microprocessor and is used for realizing a pressure detection function when executing a sample detection item.
In some embodiments, further comprising: the automatic sample introduction module is connected with the main communication interface and is used for realizing an automatic sample introduction function; the temperature control module is connected with the main communication interface and used for providing a preset temperature when executing a sample detection item; the reagent detection module is connected with the main communication interface and is used for realizing a reagent detection function; a power supply module; the automatic sample feeding device is connected with the main control module, the driving module, the automatic sample feeding module, the temperature control module and the reagent detection module respectively, and is used for supplying power to the main control module, the driving module, the automatic sample feeding module, the temperature control module and the reagent detection module.
In some embodiments, the power module comprises a switching power supply, wherein a first output of the switching power supply is coupled to the temperature control module for providing a first power supply to the temperature control module; the second output end of the switch power supply is connected with the main control module, the driving module, the automatic sample injection module and the reagent detection module through a switch respectively, and is used for providing a second power supply for the main control module, the driving module, the automatic sample injection module and the reagent detection module.
In some embodiments, the autosampler module comprises: a slave communication interface connected with the master communication interface; a third microprocessor connected to the slave communication interface; a third programmable logic array connected to the third microprocessor; the first interface is connected with the third programmable logic array and is connected with a motor; and the second interface is connected with the third programmable logic array and is connected with an optical coupler.
According to a second aspect of the present invention, a method of controlling an exemplary immunofluorescence detection system is disclosed, wherein the immunofluorescence detection system comprises: the main control module comprises a main communication interface; the driving module is connected with the main communication interface and comprises at least two driving interfaces and at least two optical coupling interfaces, wherein each driving interface is connected with at least two stepping motors, and each optical coupling interface is connected with an optical coupling; the method comprises the following steps: the main control module executes scheduling control according to the sample detection item selected by the user; and the driving module responds to the scheduling control, and generates control instructions of the corresponding stepping motor and the optical coupler according to the sample detection item selected by the user so as to control the corresponding stepping motor to execute corresponding operation and monitor the corresponding optical coupler, thereby carrying out the sample detection item.
The invention has the following beneficial effects: include two at least drive interfaces and two at least opto-coupler interfaces through drive module, every drive interface is connected with two at least step motor, and every opto-coupler interface is connected with an opto-coupler, and expansibility is strong, can support a plurality of step motor and opto-coupler. And, through the main control module including the main communication interface, can realize the upgrading update of different modules.
Drawings
FIG. 1 is a schematic structural diagram of an immunofluorescence detection system according to an embodiment of the present invention.
Fig. 2 is a schematic structural diagram of a main control module according to an embodiment of the present invention.
Fig. 3 is a schematic structural diagram of a driving module according to an embodiment of the present invention.
FIG. 4 is a schematic diagram of another immunofluorescence detection system according to an embodiment of the present invention.
Fig. 5 is a schematic structural diagram of an automatic sample injection module according to an embodiment of the present invention.
Fig. 6 is a schematic structural diagram of a temperature control module according to an embodiment of the present invention.
FIG. 7 is a schematic structural diagram of a reagent detection module according to an embodiment of the present invention.
Fig. 8 is a schematic structural diagram of a power module according to an embodiment of the present invention.
FIG. 9 is a flowchart of a control method of immunofluorescence detection according to an embodiment of the present invention.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution of the present invention is further described in detail below with reference to the accompanying drawings and the detailed description.
Fig. 1 is a schematic structural diagram of an immunofluorescence detection system according to an embodiment of the present invention. The immunofluorescence detection system 100 includes a main control module 110, a driving module 120, an automatic sample introduction module 130, a temperature control module 140, a reagent detection module 150, and a power supply module 160. The power module 160 is respectively connected to the main control module 110, the driving module 120, the autosampling module 130, the temperature control module 140 and the reagent detection module 150, and the driving module 120, the autosampling module 130, the temperature control module 140, the reagent detection module 150 and the power module 160 are also respectively connected to the main control module 110.
Specifically, the master control module 110 includes a primary communication interface 111. The driver module 120 is connected to the main communication interface 111. The driving module 120 includes at least two driving interfaces 121 and at least two optical coupling interfaces 122, where each driving interface 121 is connected to at least two stepping motors, and each optical coupling interface 122 is connected to an optical coupling.
The main control module 110 executes scheduling control according to a sample detection item selected by a user, and the driving module 120 generates a control instruction of a corresponding stepping motor and an optical coupler according to the sample detection item selected by the user in response to the scheduling control, so as to control the corresponding stepping motor to execute corresponding operation and monitor the corresponding optical coupler, thereby performing the sample detection item.
As shown in fig. 1, the automatic sample introduction module 130 is connected to the main communication interface 111, the temperature control module 140 is connected to the main communication interface 111, and the reagent detection module 150 is connected to the main communication interface 111, so as to connect the automatic sample introduction module 130, the temperature control module 140, and the reagent detection module 150 to the main control module 110. The autosampling module 130 is used to realize the autosampling function, that is, to send the sample into the instrument formed by the immunofluorescence detection system 100. The temperature control module 140 is used for providing a preset temperature when a sample test item is executed. The reagent detection module 150 is used to implement a reagent detection function.
The power module 160 is used for supplying power to the main control module 110, the driving module 120, the automatic sample introduction module 130, the temperature control module 140 and the reagent detection module 150.
It should be noted that before the user selects the sample testing item, the immunofluorescence detecting system 100 is powered on, i.e., the power module 160 is operated, and then the immunofluorescence detecting system 100 is initialized, wherein the driving module 120 and the autosampler module 130 initialize the mechanical components, the temperature control module 140 drives the heating device and the cooling device to reach the preset temperature, and the driving module 120 and the reagent detecting module 150 monitor the usage of the corresponding reagent.
In addition, when the driving module 120 responds to the scheduling control, the driving module 120 further needs to control to select the plurality of driving interfaces 121 and the optical coupler interfaces 122, so as to select the stepping motor and the optical coupler, and select the corresponding stepping motor and the corresponding optical coupler. If the sample detection item selected by the user indicates that a certain stepping motor needs to be driven, the driving module 120 needs to first control and select the driving interface 121 corresponding to the stepping motor. If the sample detection item selected by the user indicates that two stepping motors need to be driven, the driving module 120 needs to first control and select the driving interfaces 121 corresponding to the two stepping motors. In some embodiments, the selection of the stepping motor and the optocoupler can be controlled by the level output by the driving interface 121, for example, when a signal output by a certain driving interface 121 and a signal output by the corresponding optocoupler interface 122 are both low levels, it indicates that the stepping motor and the corresponding optocoupler corresponding to the driving interface 121 are selected, and then the stepping motor is driven to operate, and the corresponding optocoupler is monitored. In the present invention, the selection of the plurality of motor driver chips is not limited, and other selection modes of the plurality of motor driver chips also belong to the present invention.
In this embodiment, include two at least drive interfaces 121 and two at least opto-coupler interfaces 122 through drive module 120, every drive interface 121 is connected with two at least step motor, and every opto-coupler interface 122 is connected with an opto-coupler, and the expansibility is strong, can support a plurality of step motor and opto-coupler. Moreover, the main control module 110 includes a main communication interface 111, so that upgrading and updating of different modules can be realized.
In addition, the full-automatic immunofluorescence detection is realized through the automatic sample injection module 130, the temperature control module 140 and the reagent detection module 150.
In some embodiments, the optocouplers connected by the at least two optocoupler interfaces 122 are in one-to-one correspondence with the at least two stepper motors, each optocoupler being configured to characterize a respective stepper motor. In other embodiments, a plurality of optocouplers may also be used to characterize one stepper motor, and at this time, each stepper motor of at least two stepper motors corresponds to a plurality of optocouplers, for example, each stepper motor corresponds to two optocouplers.
The corresponding step motor performs corresponding operation, which may include generating a pulse signal, a direction control signal and/or an enable control signal according to the position of the corresponding step motor characterized by the optical coupler, and then controlling the rotation speed of the corresponding step motor according to the pulse signal, and/or controlling the rotation direction of the corresponding step motor according to the direction control signal, or controlling the start or stop of the corresponding step motor according to the enable control signal.
In some embodiments, as shown in fig. 2, a schematic structural diagram of the main control module 110 according to the embodiment of the present invention is shown. The main control module 110 includes a main communication interface 111, a first processor 112, a first Field Programmable Gate Array (FPGA) 113, and a multi-channel analog-to-digital conversion circuit (1, 2 … n), wherein the first processor 112 is connected to the main communication interface 111; the first field programmable gate array 113 is connected with the first processor 112; a plurality of Analog-to-Digital converters (ADC) (1, 2 … n) are respectively connected to the first fpga 113, wherein each Analog-to-Digital Converter (1, 2 … or n) is connected to a photosensor for performing Analog-to-Digital conversion on the fluorescence signal collected by the photosensor.
The first processor 112 may be an ARM processor. The first processor 112 performs scheduling control according to the sample test items selected by the user, thereby controlling the sample test items to be performed. The analog-to-digital conversion circuit performs analog-to-digital conversion processing on the fluorescence signal acquired by the photoelectric sensor.
As shown in fig. 2, the hosted module 110 further includes an LCD interface 114, a touch screen interface 115, a network interface 116, a first SD card circuit 117a, a second SD card circuit 117b, a USB interface 118, and an RS232 interface 119.
The first processor 112 is connected to the LCD interface 114, the touch screen interface 115, the network interface 116, the first SD card circuit 117a, the USB interface 118, and the RS232 interface 119, respectively. The first field programmable gate array 113 is connected to the second SD card circuit 117 b. The first SD card circuit 117a and the second SD card circuit 117b are connected to an SD card, respectively. The LCD interface 114 is connected with an LCD display screen, the touch screen interface 115 is connected with a touch screen, and the USB interface 118 and the RS232 interface 119 are respectively connected with the peripheral equipment of the corresponding interfaces. The USB interface 118 may be 4 xsusb. The SD card connected to the first SD card circuit 117a may store a sample detection result obtained by analyzing and processing the fluorescence signal acquired by the photosensor when the first processor 112 executes the schedule control to control the sample detection item, and the SD card connected to the second SD card circuit 117b may store an electric signal obtained by performing analog-to-digital conversion on the fluorescence signal acquired by the photosensor.
For convenience of illustration, only the part related to the present embodiment is shown above, and those skilled in the art will understand that the main control module 110 may further include other circuits, for example, for convenience of analog-to-digital conversion of the acquired fluorescence signal, the main control module 110 may further include a signal processing circuit, for example, a signal amplifying circuit connected to the analog-to-digital conversion circuit, and the like. For another example, in order to facilitate upgrade and update of the main control module 110, the main control module 110 further includes a debug interface.
In this embodiment, the main control module 110 is implemented by the first processor 112 and the first field programmable gate array 113, that is, the analog circuit and the digital circuit are separately designed, so that the influence of the digital circuit on the analog circuit can be effectively reduced, and the signal jitter generated when the digital circuit performs high-speed data communication does not affect the identification and counting of the pulse signals, thereby improving the stability and the test accuracy of the whole immunofluorescence detection system.
In some embodiments, as shown in fig. 3, the driving module 120 is a schematic structural diagram according to an embodiment of the present invention. The driver module 120 comprises at least two driver interfaces 121, at least two optical coupler interfaces 122, a slave communication interface 123, a second microprocessor 124 and a second field programmable gate array 125. Each driving interface 121 is connected with at least two stepping motors, and each optical coupling interface 122 is connected with an optical coupler. The slave communication interface 123 is connected with the master communication interface 111, and the second microprocessor 124 is connected with the slave communication interface 123, so that the second microprocessor 124 of the driving module 120 is connected with the first processor 112 of the master control module 110. The second field programmable gate array 125 is connected to the second microprocessor 124, the at least two drive interfaces 121 and the at least two opto-coupler interfaces 122.
It should be noted that the slave communication interface 123 and the master communication interface 111 represent interfaces using the same interface protocol, so that the slave communication interface 123 and the master communication interface 111 can be connected.
The second microprocessor 124 generates a control instruction of the corresponding stepper motor and the optical coupler according to the sample detection item selected by the user in response to the scheduling control of the main control module 110, so as to control the corresponding stepper motor to perform the corresponding operation and monitor the corresponding optical coupler, thereby performing the sample detection item.
As shown in fig. 3, the driving module 120 further includes a pump interface 126 and a solenoid valve interface 127, wherein the pump interface 126 is connected to a pump, and the solenoid valve interface 127 is connected to a solenoid valve. The second microprocessor 124 generates a corresponding control command according to the sample test item selected by the user in response to the scheduling control of the main control module 110, so as to control the driving pump and the solenoid valve to perform the sample test item.
For convenience of illustration, only the portion related to the present embodiment is shown above, and those skilled in the art will understand that the driving module 120 may further include a dc motor 128.
In this embodiment, include two at least drive interfaces 121 and two at least opto-coupler interfaces 122 through this drive module 120, every drive interface 121 is connected with two at least step motor, realizes controlling a plurality of step motor, and the expansibility is strong.
In addition, the driving module 120 is implemented by the second microprocessor 124 and the second field programmable gate array 125, that is, the analog circuit and the digital circuit are separately designed, so that the influence of the digital circuit on the analog circuit can be effectively reduced, and the signal jitter generated when the digital circuit performs high-speed data communication does not affect the identification and counting of the pulse signals, thereby improving the stability and the testing accuracy of the whole immunofluorescence detection system.
In some embodiments, the at least two drive interfaces 121 include six drive interfaces 121.
In some embodiments, each drive interface 121 is connected to six stepper motors.
In some embodiments, when the at least two driving interfaces 121 include six driving interfaces 121, and each driving interface 121 is connected to six stepping motors, the driving module 120 can control and drive 36 stepping motors, and the expansibility is strong.
In some embodiments, the optocouplers connected by the at least two optocoupler interfaces 122 are in one-to-one correspondence with the at least two stepper motors, each optocoupler being configured to characterize a respective stepper motor.
In some embodiments, as shown in fig. 4, a schematic structural diagram of another immunofluorescence detection system according to embodiments of the present invention is shown. In addition to the embodiment of fig. 1, the immunofluorescence detection system 100 further includes a reagent needle level detection module 170, a sampling needle level detection module 180, and a pressure detection module 190. The reagent needle liquid level detection module 170, the sampling needle liquid level detection module 180 and the pressure detection module 190 are respectively connected to the driving module 120. The reagent needle liquid level detection module 170 is configured to implement a reagent needle liquid level detection function when executing a sample detection item, the sampling needle liquid level detection module 180 is configured to implement a sampling needle liquid level detection function when executing a sample detection item, and the pressure detection module 190 is configured to implement a pressure detection function when executing a sample detection item.
In this embodiment, the reagent needle liquid level detection module 170, the sampling needle liquid level detection module 180, and the pressure detection module 190 further implement full-automatic immunofluorescence detection, and enable the immunofluorescence detection system 100 to implement various sample detection items.
In addition, the reagent needle liquid level detection module 170, the sampling needle liquid level detection module 180 and the pressure detection module 190 are respectively connected with the driving module 120, so that the main control module 110 is simplified, and the stability of the whole immunofluorescence detection system is improved.
In some embodiments, as shown in connection with FIG. 3, the reagent needle level detection module 170 and the sampling needle level detection module 180 are connected to the second FPGA 125 in the drive module 120, and the pressure detection module 190 is connected to the second microprocessor 124. The reagent needle liquid level detection module 170 and the sampling needle liquid level detection module 180 are respectively connected with the second field programmable gate array 125 in the driving module 120, and the pressure detection module 190 is connected with the second microprocessor 124, so that the stability of the whole immunofluorescence detection system is improved.
In some embodiments, as shown in fig. 5, the structure of the automatic sample feeding module 130 according to the embodiments of the present invention is schematically illustrated. The autosampler module 130 comprises a slave communication interface 131, a third microprocessor 132, a third programmable logic array 133, a first interface 134 and a second interface 135. The slave communication interface 131 is connected to the master communication interface 111. The third microprocessor 132 is connected to the slave communication interface 131, so that the third microprocessor 132 of the autosampler module 130 is connected to the first processor 112 of the main control module 110. A third programmable logic array 133 is connected to the third microprocessor 132. The first interface 134 is coupled to the third programmable logic array 133 and to a motor. The second interface 135 is connected to the third programmable logic array 133 and is optically coupled thereto.
The third microprocessor 132 receives a corresponding control signal from the main control module 110 through the communication interface 131, drives the motor connected with the first interface 134 and the optical coupler connected with the second interface 135, and the motor drives the mechanical assembly, thereby realizing the automatic sample feeding function.
It should be noted that the slave communication interface 131 and the master communication interface 111 represent interfaces using the same interface protocol, so that the slave communication interface 131 and the master communication interface 111 can be connected.
For convenience of illustration, only the portion related to the present embodiment is shown above, and those skilled in the art may understand that the autosampler module 130 may further include other circuits, for example, to facilitate upgrade and update of the autosampler module 130, the autosampler module 130 further includes a debugging interface 137. For another example, to facilitate understanding of the sample entering the interior of the instrument formed by the immunofluorescence detection system, the autosampler module 130 further includes a scan head interface 136 connected to the scan head.
In some embodiments, as shown in fig. 6, a schematic structural diagram of a temperature control module 140 according to an embodiment of the present invention is shown. The temperature control module 140 includes a slave communication interface 141, a fourth microprocessor 142, a third interface 143, and a fourth interface 144. The slave communication interface 141 is connected with the master communication interface 111, and the fourth microprocessor 142 is connected with the slave communication interface 141, so that the fourth microprocessor 142 of the temperature control module 140 is connected with the first processor 112 of the main control module 110. The third interface 143 is connected to the third microprocessor 132 and to a temperature acquisition device, such as a temperature sensor. The fourth interface 144 is connected to the fourth microprocessor 142 and to the cooling device and the heating device.
The fourth microprocessor 142 receives a corresponding control signal from the main control module 110 through the communication interface 141, and controls the operation of the cooling device and the heating device according to the temperature information collected by the temperature collecting device, thereby reaching a preset temperature.
It should be noted that the slave communication interface 141 and the master communication interface 111 represent interfaces using the same interface protocol, so that the slave communication interface 141 and the master communication interface 111 can be connected.
For convenience of illustration, only the portion related to the present embodiment is shown above, and those skilled in the art will understand that the temperature control module 140 may further include other circuits, for example, to facilitate upgrade and update of the temperature control module 140, the temperature control module 140 further includes a debugging interface 145. For another example, in order to facilitate the user to know the operation condition of the temperature control module 140, the fourth microprocessor 142 is further connected to the abnormality alarm circuit through the abnormality alarm interface 146.
In some embodiments, as shown in fig. 7, a schematic structural diagram of a reagent detection module 150 according to an embodiment of the present invention is shown. The reagent detection module 150 includes a slave communication interface 151, a fifth microprocessor 152, and a fifth interface 153. The slave communication interface 151 is connected to the master communication interface 111. The fifth microprocessor 152 is connected to the slave communication interface 151, thereby enabling the fifth microprocessor 152 of the reagent detecting module 150 to be connected to the first processor 112 of the main control module 110. The fifth interface 153 is connected to the reagent detection microprocessor and to the liquid sensor.
The fifth microprocessor 152 receives a corresponding control signal from the main control module 110 through the communication interface 151, and obtains information sensed by the liquid sensing sensor, thereby monitoring the use condition of the reagent.
It should be noted that the slave communication interface 151 and the master communication interface 111 represent interfaces using the same interface protocol, so that the slave communication interface 151 and the master communication interface 111 can be connected.
For ease of illustration, only the portions related to the present embodiment are shown above, and those skilled in the art will understand that the reagent detection module 150 may further include other circuits, for example, to facilitate upgrade and update of the reagent detection module 150, the reagent detection module 150 further includes a debugging interface 154.
In some embodiments, as shown in fig. 8, a schematic structural diagram of the power module 160 according to an embodiment of the present invention is shown. The power module 160 includes a switching power supply 161, wherein a first output terminal of the switching power supply 161 and the temperature control module 140 are configured to provide a first power supply to the temperature control module 140; a second output end of the switching power supply 161 is connected to the main control module 110, the driving module 120, the automatic sample injection module 130, and the reagent detection module 150 through a switch 162, and is configured to provide a second power supply for the main control module 110, the driving module 120, the automatic sample injection module 130, and the reagent detection module 150.
In this embodiment, the power module 160 is simple and realizes power supply of the whole immunofluorescence detection system through a single switching power supply.
Fig. 9 is a flowchart of a control method of immunofluorescence detection according to an embodiment of the present invention. The control method is applied to the immunofluorescence detection system in the above embodiment. For details of the immunofluorescence detection system, see the description of the above embodiments, and are not repeated herein. The method comprises the following steps:
step 910: the main control module 110 performs scheduling control according to the sample test item selected by the user.
Step 920: the driving module 120 responds to the scheduling control, and generates a control instruction of the corresponding stepping motor and the optical coupler according to the sample detection item selected by the user, so as to control the corresponding stepping motor to execute the corresponding operation and monitor the corresponding optical coupler, thereby performing the sample detection item.
In some embodiments, the respective stepping motor performs the respective operation, which may include generating a pulse signal, a direction control signal, and/or an enable control signal according to a position of the respective stepping motor characterized by the optical coupler, and then controlling a rotation speed of the respective stepping motor according to the pulse signal, and/or controlling a rotation direction of the respective stepping motor according to the direction control signal, or controlling a start or stop operation of the respective stepping motor according to the enable control signal.
It will be apparent to those skilled in the art that many modifications and variations can be made in the apparatus and method while maintaining the teachings of the present disclosure. Accordingly, the above disclosure should be considered limited only by the scope of the following claims.
Claims (10)
1. An immunofluorescence detection system, comprising:
the main control module comprises a main communication interface;
the driving module is connected with the main communication interface and comprises at least two driving interfaces and at least two optical coupling interfaces, wherein each driving interface is connected with at least two stepping motors, and each optical coupling interface is connected with an optical coupling;
the main control module executes scheduling control according to a sample detection item selected by a user, and the driving module responds to the scheduling control and generates control instructions of corresponding stepping motors and the optical couplers according to the sample detection item selected by the user so as to control the corresponding stepping motors to execute corresponding operations and monitor the corresponding optical couplers, so that the sample detection item is carried out.
2. The immunofluorescence detection system according to claim 1, wherein the master control module further comprises:
a first processor connected with the primary communication interface;
the first field programmable gate array is connected with the first processor;
and the multiple analog-to-digital conversion circuits are respectively connected with the first field programmable gate array, and each analog-to-digital conversion circuit is connected with one photoelectric sensor and is used for performing analog-to-digital conversion processing on the fluorescence signal acquired by the photoelectric sensor.
3. The immunofluorescence detection system according to claim 1, wherein the drive module further comprises:
a slave communication interface connected with the master communication interface;
the second microprocessor is connected with the slave communication interface;
and the second field programmable gate array is connected with the second microprocessor, the at least two driving interfaces and the at least two optical coupling interfaces.
4. The immunofluorescence detection system according to claim 3, wherein the at least two drive interfaces comprises six drive interfaces.
5. The immunofluorescence detection system according to claim 3, wherein each of the drive interfaces is connected to six stepper motors.
6. The immunofluorescence detection system according to any one of claims 4 or 5, further comprising:
the reagent needle liquid level detection module is connected with the second field programmable gate array and used for realizing the reagent needle liquid level detection function when executing the sample detection items;
the sampling needle liquid level detection module is connected with the second field programmable gate array and used for realizing the sampling needle liquid level detection function when executing a sample detection item; and
and the pressure detection module is connected with the second microprocessor and is used for realizing a pressure detection function when executing a sample detection item.
7. The immunofluorescence detection system according to claim 1, further comprising:
the automatic sample introduction module is connected with the main communication interface and is used for realizing an automatic sample introduction function;
the temperature control module is connected with the main communication interface and used for providing a preset temperature when executing a sample detection item;
the reagent detection module is connected with the main communication interface and is used for realizing a reagent detection function;
a power supply module; the automatic sample feeding device is connected with the main control module, the driving module, the automatic sample feeding module, the temperature control module and the reagent detection module respectively, and is used for supplying power to the main control module, the driving module, the automatic sample feeding module, the temperature control module and the reagent detection module.
8. The immunofluorescence detection system according to claim 7, wherein the power module comprises a switching power supply, wherein a first output of the switching power supply and the temperature control module are configured to provide a first power supply to the temperature control module; the second output end of the switch power supply is connected with the main control module, the driving module, the automatic sample injection module and the reagent detection module through a switch respectively, and is used for providing a second power supply for the main control module, the driving module, the automatic sample injection module and the reagent detection module.
9. The immunofluorescence detection system according to claim 8, wherein the autosampler module comprises:
a slave communication interface connected with the master communication interface;
a third microprocessor connected to the slave communication interface;
a third programmable logic array connected to the third microprocessor;
the first interface is connected with the third programmable logic array and is connected with a motor;
and the second interface is connected with the third programmable logic array and is connected with an optical coupler.
10. A method for controlling an immunofluorescence detection system, wherein the immunofluorescence detection system comprises:
the main control module comprises a main communication interface;
the driving module is connected with the main communication interface and comprises at least two driving interfaces and at least two optical coupling interfaces, wherein each driving interface is connected with at least two stepping motors, and each optical coupling interface is connected with an optical coupling;
the method comprises the following steps:
the main control module executes scheduling control according to the sample detection item selected by the user; and the driving module responds to the scheduling control, and generates control instructions of the corresponding stepping motor and the optical coupler according to the sample detection item selected by the user so as to control the corresponding stepping motor to execute corresponding operation and monitor the corresponding optical coupler, thereby carrying out the sample detection item.
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