CN112433063A - Full-automatic chemiluminescence immunoassay technical platform - Google Patents

Full-automatic chemiluminescence immunoassay technical platform Download PDF

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CN112433063A
CN112433063A CN202011238309.6A CN202011238309A CN112433063A CN 112433063 A CN112433063 A CN 112433063A CN 202011238309 A CN202011238309 A CN 202011238309A CN 112433063 A CN112433063 A CN 112433063A
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control module
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matrix
sample
reaction
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CN112433063B (en
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李开通
贾成成
白卉
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Zhaoyan Beijing Pharmaceutical Technology Co ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N2035/00891—Displaying information to the operator

Abstract

The invention relates to a full-automatic chemiluminescence immunoassay technical platform which comprises a reaction cup, a sample detector, a first reaction bin, a second reaction bin, a photoelectric receiver, a photomultiplier, a central control module, a display screen and a control panel. The invention is a direct chemiluminescence technology based on a magnetic bead method through a fully-automatic chemiluminescence immunoassay technology platform to be established, has the advantages of relatively low equipment cost, simple and convenient operation, high analysis speed, high sensitivity, strong specificity, wide linear range, wide application range and the like, can realize full-automatic and high-flux biological sample analysis, simultaneously has high-efficiency and stable labeled molecules, high-efficiency and stable excitant and perfect and mature labeling technology basis, and effectively solves the problems of low sensitivity, narrow linear range, poor stability, and more importantly, low automation degree and flux level of the traditional immunoassay method.

Description

Full-automatic chemiluminescence immunoassay technical platform
Technical Field
The invention relates to the technical field of immunodetection, in particular to a full-automatic chemiluminescence immunodetection technical platform.
Background
The immunoassay is carried out in different stages such as Radioimmunoassay (RIA), fluoroimmunoassay (IFA), enzyme-labeled immunoassay (EIA), etc., and chemiluminescence immunoassay (CLIA) is a new stage of immunoassay development. Chemiluminescence immunoassay is applied in 80 s of the 20 th century, and is established by combining a high-sensitivity chemiluminescence technology with high-specificity immunoreaction according to the basic principle of immunoassay.
Compared with radioimmunoassay, fluoroimmunoassay and enzyme immunoassay, CLIA combines the advantages of chemiluminescence and immunity, has the characteristics of no radiation hazard, high sensitivity, strong specificity, wide linear range, simple and convenient operation, high analysis speed, long effective period of a marker, no need of expensive instruments and equipment, realization of full automation, high flux and the like, has wide application range, and is suitable for detection of biological medicines such as monoclonal antibodies, fusion proteins, polypeptides, nucleic acids and the like. The method has been widely applied in the field of clinical examination and becomes a support method for clinical immunodiagnosis, but is limited by the detection platform and the closure of a detection reagent system, and is rarely applied in the research of drug metabolism and immunogenicity in preclinical and clinical stages at present.
However, in the field of the current drug metabolism research and immunogenicity research in preclinical and clinical stages, traditional enzyme immunoassay and radioimmunoassay are mostly used, and the detection efficiency is low due to low sensitivity, narrow linear range and poor stability.
Disclosure of Invention
Therefore, the invention provides a full-automatic chemiluminescence immunoassay technical platform, which is used for overcoming the problem of low immunoassay efficiency in the prior art.
The invention provides a full-automatic chemiluminescence immunoassay technical platform, which comprises:
the reaction cup is used for containing the biological sample to be detected;
the first reaction bin is used for selecting corresponding reaction types according to the types of the samples to expose macromolecules of the biological sample and carrying out qualitative or quantitative analysis on the macromolecules;
the second reaction bin is connected with the first reaction bin through a conveyor belt and is used for carrying out magnetic bead coupling on macromolecules in a biological sample, adding a chemiluminescence substance and excitation liquid and detecting a reaction result;
the sample detector is arranged above the first reaction bin and is used for detecting the type of a sample;
the photoelectric receiver is arranged above the second reaction chamber and is used for collecting a luminous signal of the biological sample in the second reaction chamber;
the photomultiplier is connected with the photoelectric receiver and is used for converting an optical signal of the photoelectric receiver into an electric signal and amplifying the electric signal;
the central control module is respectively connected with the sample type detector, the first reaction bin, the second reaction bin, the photoelectric receiver and the photomultiplier and is used for adjusting the working state of each part;
the display screen is connected with the central control module and is used for displaying the working state of each part and the sample detection result;
the control panel is connected with the central control module and used for manually selecting and adjusting the working state of each part;
a sample species matrix A0, an exposed macromolecule reaction mode matrix B0, a to-be-detected item magnetic bead addition quantity matrix C0, a chemiluminescence substance addition quantity matrix D0 and an excitation liquid species matrix E0 are arranged in the central control module;
when the detection platform detects a sample to be detected, the sample is placed into a reaction cup, the reaction cup is placed into a first reaction chamber, the sample detector detects the type of the sample and transmits the detection result to a central control module, the central control module selects a corresponding macromolecule exposure reaction mode according to the detection result, the first reaction chamber exposes macromolecules in the sample and measures the macromolecule content value F of the sample, the central control module controls the conveyor belt to move the reaction cup from the first reaction chamber to a second reaction chamber, an operator selects detection items through the control module, and the central control module respectively selects corresponding preset magnetic bead addition quantity Cij, preset luminescent material addition quantity Dij and excitation liquid type Ej according to the detection items and adjusts the actual magnetic bead addition quantity value through F;
when the magnetic beads which are measured as Cij 'are added into the reaction cup and coupled for a preset time length T through the magnetic beads, the second reaction bin detects the coupling completion degree G and transmits the detection result to the central control module, and the central control module calculates the coupling compensation time length T' according to the completion degree G;
when the magnetic bead coupling reaction is complete, the central control module adjusts the adding amount of a preset luminescent substance to Dij 'according to the adding amount Cij' of the magnetic beads, the luminescent substance and a sample to be detected are fully mixed, the second reaction chamber adds sufficient Ej exciting liquid into the reaction cup to enable the sample to be detected to react and emit light, the photoelectric receiver receives a luminescent signal and transmits the received optical signal to the photoelectric multiplier tube, the photoelectric multiplier tube converts the optical signal into an electric signal Q and amplifies the electric signal, and the amplified electric signal is Q.
Furthermore, an electric signal value matrix Q0 and an electric signal secondary adjustment parameter matrix m0 are arranged in the central control module,
for the matrix of electrical signal values Q0, Q0(Q1, Q2), where Q1 is the first preset electrical signal value, Q2 is the second preset electrical signal value, Q1 < Q2;
for the electrical signal secondary adjustment parameter matrix m0, m0(m1, m2), wherein m1 is a first preset electrical signal secondary adjustment parameter, and m2 is a second preset electrical signal secondary adjustment parameter;
the central control module compares the Q and the QO matrix internal parameters and adjusts the electric signal Q according to the comparison result:
when Q is not more than Q1, the central control module judges that the electric signal Q is too small and does not meet the data processing requirement, the central control module selects m1 from the m0 matrix as the secondary adjustment parameter of the electric signal and adjusts the electric signal to Q', Q ═ Q × m 1;
when Q is more than Q1 and less than or equal to Q2, the central control module judges that the electric signal Q meets the data processing requirement;
when Q is more than Q1, the central control module judges that the electric signal Q is too large and does not meet the data processing requirement, and the central control moduleThe block selects m2 from the m0 matrix as the secondary adjustment parameter of the electrical signal and adjusts the electrical signal to Q',
Figure BDA0002767551170000031
when the central control module adjusts the electric signal to be Q ', the central control module compares the Q' with the internal parameters of the QO matrix, and when Q1 is more than Q 'and less than or equal to Q2, the central control module judges that the electric signal Q' meets the data processing requirements; when Q 'is not in the range of Q1-Q2, the operation is repeated until Q1 is less than Q' ≦ Q2.
Further, for item magnetic bead addition quantity matrix groups to be detected, C0 and C0(C1, C2 and C3), wherein C1 is a first type sample item magnetic bead addition quantity matrix to be detected, C2 is a second type sample item magnetic bead addition quantity matrix to be detected, and C3 is a third type sample item magnetic bead addition quantity matrix to be detected;
for an i-th type sample to-be-detected item magnetic bead addition quantity matrix Ci, Ci (Ci1, Ci2, Ci3 and Ci4), wherein Ci1 is the first detection item magnetic bead addition quantity of the i-th type sample, Ci2 is the second detection item magnetic bead addition quantity of the i-th type sample, Ci3 is the third detection item magnetic bead addition quantity of the i-th type sample, and Ci4 is the fourth detection item magnetic bead addition quantity of the i-th type sample;
the central control module is also provided with a coupling completion degree matrix G0 and a magnetic bead coupling duration compensation parameter matrix t 0;
for the coupling completion degree matrices G0, G0(G1, G2, G3), wherein G1 is the first predetermined coupling completion degree, G2 is the second predetermined coupling completion degree, G3 is the third predetermined coupling completion degree, the completion degree values sequentially increase;
for a magnetic bead coupling duration compensation parameter matrix t0, t0(t1, t2, t3), wherein t1 is a first preset magnetic bead coupling duration compensation parameter, t2 is a second preset magnetic bead coupling duration compensation parameter, t3 is a third preset magnetic bead coupling duration compensation parameter, and the values of the compensation parameters are sequentially reduced;
the central control module adds Cij' measuring magnetic beads into the reaction cup, when the preset time length T is coupled through the magnetic beads, the second reaction bin detects the coupling completion degree G and transmits the detection result to the central control module, and the central control module compares the G with the internal parameters of the G0 matrix:
when G is less than or equal to G1, the central control module judges that the coupling of the magnetic beads is incomplete and selects t1 from the t0 matrix as a coupling duration compensation parameter;
when G is more than G1 and less than or equal to G2, the central control module judges that the coupling of the magnetic beads is incomplete and selects t2 from a t0 matrix as a coupling duration compensation parameter;
when G is more than G2 and less than or equal to G3, the central control module judges that the coupling of the magnetic beads is incomplete and selects t3 from a t0 matrix as a coupling duration compensation parameter;
when G is larger than G3, the central control module judges that the magnetic beads are completely coupled;
when the central control module judges that the coupling of the magnetic beads is incomplete and selects tp as a coupling time length compensation parameter, p is 1,2 and 3, and the central control module calculates the coupling compensation time length T ', T' is T multiplied by tp; when the coupling compensation time length T ' passes, the second reaction bin detects the coupling completion degree G ', and the operation is repeated until G ' is more than G3;
when the magnetic bead coupling reaction is complete, the central control module adjusts the adding amount of the preset luminescent substance to Dij 'according to the adding amount of the magnetic bead Cij',
Figure BDA0002767551170000041
wherein L is a compensation parameter for adjusting the addition amount of the magnetic beads to the addition amount of the luminescent material.
Further, for the sample type matrix a0, a0(a1, a2, A3), where a1 is a first predetermined sample type, a2 is a second predetermined sample type, and A3 is a third predetermined sample type;
exposing macromolecular reaction pattern matrixes B0 and B0(B1, B2 and B3), wherein B1 is a first preset reaction pattern, B2 is a second preset reaction pattern, and B3 is a third preset reaction pattern;
the reaction cup filled with the biological sample to be detected is placed into a first detection bin, the sample detector detects the type A of the biological sample in the first reaction bin and transmits a detection result to a central control module, and the central control module compares the A with internal parameters of a matrix A0:
when A is A1, the central control module selects B1 from the matrix B0 as an exposure macromolecule reaction mode;
when A is A2, the central control module selects B2 from the matrix B0 as an exposure macromolecule reaction mode;
when A is A3, the central control module selects B3 from the matrix B0 as an exposure macromolecule reaction mode;
when the macromolecules of the biological sample are fully exposed, the first reaction chamber quantifies the macromolecules, and the determination content value is F.
Further, for the chemiluminescence substance adding amount matrix groups D0 and D0(D1, D2 and D3), wherein D1 is a first type sample illuminant adding amount matrix, D2 is a second type sample illuminant adding amount matrix, and D3 is a third type sample illuminant adding amount matrix;
for the ith type sample illuminant addition quantity matrix Di, i is 1,2,3, Di (Di1, Di2, Di3, Di4), wherein Di1 is the illuminant addition quantity of the first detection item of the ith type sample, Di2 is the illuminant addition quantity of the second detection item of the ith type sample, Di3 is the illuminant addition quantity of the third detection item of the ith type sample, and Di4 is the illuminant addition quantity of the fourth detection item of the ith type sample;
for the excitation liquid type matrix E0, E0(E1, E2, E3, E4), where E1 is the first type excitation liquid, E2 is the second detection item excitation liquid, E3 is the third detection item excitation liquid, and E4 is the fourth detection item excitation liquid;
the central control module is also provided with a macromolecule content value matrix F0(F1, F2, F3 and F4), wherein F1 is a first preset macromolecule content value, F2 is a second preset macromolecule content value, F3 is a third preset macromolecule content value, F4 is a fourth preset macromolecule content value, and the content values are sequentially increased;
when the jth item of detection is carried out on the ith type sample, j is 1,2,3 and 4, and the central control module selects Cij as the preset magnetic bead addition amount from the magnetic bead addition amount matrix Ci to be detected on the ith type sample; selecting Dij from the Di matrix as a preset illuminant addition amount; ej is selected from the matrix E0 as the excitation liquid species.
The central control module compares the parameters in the F and F0 matrixes to adjust the addition amount of the magnetic beads:
when F is less than or equal to F1, the central control module selects c1 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is more than F1 and less than or equal to F2, the central control module selects c2 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is more than F2 and less than or equal to F3, the central control module does not adjust the addition amount of the magnetic beads;
when F is more than F3 and less than or equal to F4, the central control module selects c3 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is larger than F4, the central control module selects c4 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when the central control module selects ck to adjust the addition quantity Cij of the magnetic beads, k is 1,2,3 and 4, and the central control module adjusts the addition quantity of the magnetic beads to Cij 'and Cij' is Cij multiplied by ck.
Further, when the luminophor in the reaction cup is sufficiently mixed with the sample to be detected, the central control module controls the second reaction bin to add sufficient Ej exciting liquid into the reaction cup, the chemiluminescence object absorbs immune reaction and then emits light, the photoelectric receiver receives a light-emitting signal and transmits the received light signal to the photomultiplier, and the photomultiplier converts the light signal into an electric signal q and amplifies the electric signal q;
the central control module is provided with signal amplification matrix groups M0 and M0(M1, M2 and M3), wherein M1 is a first type sample signal amplification matrix, M2 is a second type sample signal amplification matrix, and M3 is a third type sample signal amplification matrix;
for the ith type sample signal amplification matrix Mi, i is 1,2,3,4, Mi (Mi1, Mi2, Mi3, Mi4), where Mi1 is the first preset signal amplification parameter of the ith type sample, Mi2 is the second preset signal amplification parameter of the ith type sample, Mi3 is the third preset signal amplification parameter of the ith type sample, and Mi4 is the fourth preset signal amplification parameter of the ith type sample;
when the jth item of detection is carried out on the ith type of sample, the central control module selects Mij from the M0 matrix group as an electric signal amplification parameter, and amplifies the electric signal to Q, wherein Q is Q multiplied by Mij.
Further, when the electric signal Q' meets the data processing requirement, the central control module carries out data processing on the telecommunication to generate a detection result and displays the detection result through the display screen.
Further, the detection technology platform can detect a plurality of samples at one time, and the total number of the samples is not more than 200.
Furthermore, operating end software is arranged in the central control module, and the software functions comprise: user login, password change, role assignment authority, real-time display of the state of the technical platform, import and confirmation of the reagent configuration file, and after the detection result is generated, the software can automatically generate the standard song according to the result.
Compared with the prior art, the full-automatic chemiluminescence immunoassay technical platform is a direct chemiluminescence technology based on a magnetic bead method, has the advantages of relatively low equipment cost, simplicity and convenience in operation, high analysis speed, high sensitivity, strong specificity, wide linear range, wide application range and the like, can realize full-automatic and high-flux biological sample analysis, and simultaneously has efficient and stable labeled molecules, efficient and stable excitants and perfect and mature labeling technical basis. The sensitivity of the immunoassay method is effectively improved, the linear range is widened, and the stability is improved, so that the immunoassay efficiency is improved.
Further, a sample species matrix A0, an exposed macromolecule reaction mode matrix B0, a to-be-detected item magnetic bead addition quantity matrix C0, a chemiluminescence object addition quantity matrix D0 and an excitation liquid species matrix E0 are arranged in the central control module; when a certain sample is to be detected, the sample is placed into a reaction cup, the reaction cup is placed into a first reaction chamber, a sample detector detects the type of the sample and transmits the detection result to a central control module, the central control module selects a corresponding macromolecule exposure reaction mode according to the detection result, the first reaction chamber exposes macromolecules and measures a content value F, an operator selects a detection item through a control module, the central control module selects a preset magnetic bead addition quantity Cij, a preset luminescent material addition quantity Dij and an exciting liquid type Ej according to the detection item and adjusts an actual value of the magnetic bead addition quantity through F, an accurate value of the magnetic bead addition quantity is determined, the stability of the detection result is further improved, and the immunodetection efficiency is improved.
Further, when the magnetic beads which are measured as Cij 'are added into the reaction cup and coupled through the magnetic beads for a preset time length T, the second reaction chamber detects the coupling completion degree G and transmits the detection result to the central control module, the central control module calculates the coupling compensation time length T' according to the completion degree G, the reaction time length is compensated through detecting the coupling completion degree of the magnetic beads, the coupling completion degree is improved, the waiting time is shortened, and the immunodetection efficiency is further improved.
Further, when the magnetic bead coupling reaction is complete, the central control module adjusts the addition amount of a preset luminescent material to Dij 'according to the addition amount of the magnetic beads Cij', the luminescent material and a sample to be detected are fully mixed, the second reaction chamber adds sufficient Ej exciting liquid into the reaction cup to enable the sample to be detected to react and emit light, the photoelectric receiver receives a luminescent signal and transmits the received optical signal to the photoelectric multiplier tube, the photoelectric multiplier tube converts the optical signal into an electric signal Q and amplifies the electric signal to Q, the central control module compares the Q with the QO matrix internal parameters and adjusts the electric signal Q according to a comparison result, the electric signal Q is adjusted to an optimal analysis range, the sensitivity of the immunoassay method is further improved, and the immunoassay efficiency is improved.
Further, the detection technology platform can detect a plurality of samples at one time, the total number of the samples is not more than 200, and the plurality of samples are detected simultaneously, so that the immunodetection efficiency is further improved.
Furthermore, when the electric signal Q' meets the data processing requirement, the central control module performs data processing on the telecommunication to generate a detection result and displays the detection result through the display screen, and after the detection result is generated, the central control module can automatically generate a standard curve according to the result, clearly reflects the detection result data and further improves the immunodetection efficiency.
Drawings
FIG. 1 is a schematic structural diagram of a full-automatic chemiluminescence immunoassay platform.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described below with reference to examples; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and do not limit the scope of the present invention.
It should be noted that in the description of the present invention, the terms of direction or positional relationship indicated by the terms "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Fig. 1 is a schematic structural diagram of a full-automatic chemiluminescence immunoassay platform according to the present invention. The invention relates to a full-automatic chemiluminescence immunoassay technical platform, which comprises: including reaction cup 1, sample detector 2, first reaction storehouse 3, second reaction storehouse 4, photoelectric receiver 5, photomultiplier 6, well accuse module 7, display screen 8 and control panel 9, wherein: the reaction cup 1 is used for containing a biological sample to be detected; the sample detector 2 is used for detecting the sample type; the first reaction chamber 3 is used for selecting corresponding reaction types according to the types of the samples to expose macromolecules of the biological sample and carrying out qualitative or quantitative analysis on the macromolecules; the second reaction chamber 4 is used for performing magnetic bead coupling on macromolecules in the biological sample, adding a chemiluminescence substance and excitation liquid, and detecting a reaction result; the photoelectric receiver 5 is arranged above the second reaction chamber 4 and is used for collecting a luminous signal of the biological sample in the second reaction chamber 4; the photomultiplier 6 is used for converting the optical signal of the photoelectric receiver 5 into an electric signal and amplifying the electric signal; the central control module 7 is respectively connected with the sample type detector, the first reaction bin 3, the second reaction bin, the photoelectric receiver 5 and the photomultiplier 6 and is used for adjusting the working states of all the components; the display screen 8 is connected with the central control module 7 and used for displaying the working states of all the parts and the sample detection result; the control panel 9 is connected with the central control module 7 and used for manually selecting and adjusting the working state of each part.
A sample species matrix A0, an exposed macromolecule reaction mode matrix B0, a matrix group C0 for adding magnetic beads of items to be detected, a matrix group D0 for adding chemiluminescence substances and an excitation liquid species matrix E0 are arranged in the central control module 7;
when the detection platform detects a sample to be detected, the sample is placed into the reaction cup 1, the reaction cup 1 is placed into the first reaction chamber 3, the sample detector 2 detects the type of the sample and transmits the detection result to the central control module 7, the central control module 7 selects a corresponding macromolecule exposure reaction mode according to the detection result, the first reaction chamber 3 exposes macromolecules in the sample and measures a macromolecule content value F of the sample, the central control module 7 controls the conveyor belt to move the reaction cup 1 from the first reaction chamber 3 to the second reaction chamber 4, an operator selects detection items through the control module, and the central control module 7 selects corresponding preset addition amount magnetic beads Cij, preset luminescent material addition amount Dij and excitation liquid type Ej according to the detection items and adjusts the actual addition amount value of the magnetic beads through F;
when the magnetic beads which are measured as Cij 'are added into the reaction cup 1 and coupled through the magnetic beads for a preset time length T, the second reaction bin 4 detects the coupling completion degree G and transmits the detection result to the central control module 7, and the central control module 7 calculates the coupling compensation time length T' according to the completion degree G;
when the magnetic bead coupling reaction is complete, the central control module 7 adjusts the adding amount of a preset luminescent material to Dij 'according to the adding amount Cij' of the magnetic beads, the luminescent material and a sample to be detected are fully mixed, the second reaction chamber 4 adds sufficient Ej exciting liquid into the reaction cup 1 to enable the sample to be detected to react and emit light, the photoelectric receiver 5 receives a luminescent signal and transmits the received optical signal to the photomultiplier 6, the photomultiplier 6 converts the optical signal into an electric signal Q and amplifies the electric signal, and the amplified electric signal is Q.
Specifically, an electric signal value matrix Q0 and an electric signal secondary adjustment parameter matrix m0 are also arranged in the central control module 7,
for the matrix of electrical signal values Q0, Q0(Q1, Q2), where Q1 is the first preset electrical signal value, Q2 is the second preset electrical signal value, Q1 < Q2;
for the electrical signal secondary adjustment parameter matrix m0, m0(m1, m2), wherein m1 is a first preset electrical signal secondary adjustment parameter, and m2 is a second preset electrical signal secondary adjustment parameter;
the central control module 7 compares the Q and the QO matrix internal parameters and adjusts the electric signal Q according to the comparison result:
when Q is not more than Q1, the central control module 7 determines that the electric signal Q is too small and does not meet the data processing requirements, the central control module 7 selects m1 from the m0 matrix as the secondary adjustment parameter of the electric signal and adjusts the electric signal to Q ', Q' ═ Q × m 1;
when Q is more than Q1 and less than or equal to Q2, the central control module 7 judges that the electric signal Q meets the data processing requirement;
when Q is more than Q1, the central control module 7 judges that the electric signal Q is too large and does not meet the data processing requirement, the central control module 7 selects m2 from the m0 matrix as the secondary adjustment parameter of the electric signal and adjusts the electric signal to be Q',
Figure BDA0002767551170000091
when the central control module 7 adjusts the electric signal to be Q ', the central control module 7 compares the Q' with the parameters in the QO matrix, and when Q1 is more than Q 'and less than or equal to Q2, the central control module 7 judges that the electric signal Q' meets the data processing requirement; when Q 'is not in the range of Q1-Q2, the operation is repeated until Q1 is less than Q' ≦ Q2.
Specifically, for item magnetic bead addition quantity matrix groups to be detected, C0 and C0(C1, C2 and C3), wherein C1 is a first type sample item magnetic bead addition quantity matrix to be detected, C2 is a second type sample item magnetic bead addition quantity matrix to be detected, and C3 is a third type sample item magnetic bead addition quantity matrix to be detected;
for an i-th type sample to-be-detected item magnetic bead addition quantity matrix Ci, Ci (Ci1, Ci2, Ci3 and Ci4), wherein Ci1 is the first detection item magnetic bead addition quantity of the i-th type sample, Ci2 is the second detection item magnetic bead addition quantity of the i-th type sample, Ci3 is the third detection item magnetic bead addition quantity of the i-th type sample, and Ci4 is the fourth detection item magnetic bead addition quantity of the i-th type sample;
the central control module 7 is further provided with a coupling completion degree matrix G0 and a magnetic bead coupling duration compensation parameter matrix t 0;
for the coupling completion degree matrices G0, G0(G1, G2, G3), wherein G1 is the first predetermined coupling completion degree, G2 is the second predetermined coupling completion degree, G3 is the third predetermined coupling completion degree, the completion degree values sequentially increase;
for a magnetic bead coupling duration compensation parameter matrix t0, t0(t1, t2, t3), wherein t1 is a first preset magnetic bead coupling duration compensation parameter, t2 is a second preset magnetic bead coupling duration compensation parameter, t3 is a third preset magnetic bead coupling duration compensation parameter, and the values of the compensation parameters are sequentially reduced;
the central control module 7 adds the magnetic beads with the quantity of Cij' into the reaction cup 1, when the preset time length T is coupled through the magnetic beads, the second reaction chamber 4 detects the coupling completion degree G and transmits the detection result to the central control module 7, and the central control module 7 compares G with the internal parameters of the G0 matrix:
when G is less than or equal to G1, the central control module 7 judges that the coupling of the magnetic beads is incomplete and selects t1 from the t0 matrix as a coupling duration compensation parameter;
when G is more than G1 and less than or equal to G2, the central control module 7 judges that the coupling of the magnetic beads is incomplete and selects t2 from a t0 matrix as a coupling duration compensation parameter;
when G is more than G2 and less than or equal to G3, the central control module 7 judges that the coupling of the magnetic beads is incomplete and selects t3 from a t0 matrix as a coupling duration compensation parameter;
when G is larger than G3, the central control module 7 judges that the magnetic beads are completely coupled;
when the central control module 7 judges that the coupling of the magnetic beads is incomplete and selects tp as a coupling time length compensation parameter, p is 1,2 and 3, and the central control module 7 calculates a coupling compensation time length T ', T' is T multiplied by tp; when the coupling compensation time length T ' passes, the second reaction chamber 4 detects the coupling completion degree G ', and the operation is repeated until G ' is more than G3;
when the magnetic bead coupling reaction is complete, the central control module 7 adjusts the adding amount of the preset luminescent substance to Dij 'according to the adding amount of the magnetic beads Cij',
Figure BDA0002767551170000101
wherein L is a compensation parameter for adjusting the addition amount of the magnetic beads to the addition amount of the luminescent material.
Specifically, for the sample species matrix a0, a0(a1, a2, A3), where a1 is a first predetermined sample species, a2 is a second predetermined sample species, and A3 is a third predetermined sample species;
exposing macromolecular reaction pattern matrixes B0 and B0(B1, B2 and B3), wherein B1 is a first preset reaction pattern, B2 is a second preset reaction pattern, and B3 is a third preset reaction pattern;
the reaction cup 1 filled with the biological sample to be detected is placed into a first detection bin, the sample detector 2 detects the type A of the biological sample in the first reaction bin 3 and transmits the detection result to the central control module 7, and the central control module 7 compares the A with the internal parameters of the matrix A0:
when A is A1, the central control module 7 selects B1 from the matrix B0 as an exposure macromolecule reaction mode;
when A is A2, the central control module 7 selects B2 from the matrix B0 as an exposure macromolecule reaction mode;
when A is A3, the central control module 7 selects B3 from the matrix B0 as an exposure macromolecule reaction mode;
when the macromolecules of the biological sample are fully exposed, the first reaction chamber 3 quantifies the macromolecules, and the determination content value is F.
Specifically, for the chemiluminescence substance adding amount matrix groups D0 and D0(D1, D2 and D3), wherein D1 is a first type sample illuminant substance adding amount matrix, D2 is a second type sample illuminant substance adding amount matrix, and D3 is a third type sample illuminant substance adding amount matrix;
for the ith type sample illuminant addition quantity matrix Di, i is 1,2,3, Di (Di1, Di2, Di3, Di4), wherein Di1 is the illuminant addition quantity of the first detection item of the ith type sample, Di2 is the illuminant addition quantity of the second detection item of the ith type sample, Di3 is the illuminant addition quantity of the third detection item of the ith type sample, and Di4 is the illuminant addition quantity of the fourth detection item of the ith type sample;
for the excitation liquid type matrix E0, E0(E1, E2, E3, E4), where E1 is the first type excitation liquid, E2 is the second detection item excitation liquid, E3 is the third detection item excitation liquid, and E4 is the fourth detection item excitation liquid;
the central control module 7 is further provided with a macromolecule content value matrix F0(F1, F2, F3, F4), wherein F1 is a first preset macromolecule content value, F2 is a second preset macromolecule content value, F3 is a third preset macromolecule content value, F4 is a fourth preset macromolecule content value, and the content values are sequentially increased;
when the jth item of the ith type sample is detected, j is 1,2,3 and 4, and the central control module 7 selects Cij as the preset magnetic bead addition amount from the magnetic bead addition amount matrix Ci of the item to be detected of the ith type sample; selecting Dij from the Di matrix as a preset illuminant addition amount; selecting Ej from the matrix E0 as the type of the excitation liquid;
the central control module 7 compares the parameters in the F and F0 matrixes to adjust the addition amount of the magnetic beads:
when F is less than or equal to F1, the central control module 7 selects c1 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is more than F1 and less than or equal to F2, the central control module 7 selects c2 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is more than F2 and less than or equal to F3, the central control module 7 does not adjust the addition amount of the magnetic beads;
when F is more than F3 and less than or equal to F4, the central control module 7 selects c3 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is larger than F4, the central control module 7 selects c4 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when the central control module 7 selects ck to adjust the magnetic bead addition amount Cij, k is 1,2,3,4, and the central control module 7 adjusts the magnetic bead addition amount Cij' to Cij × ck.
Specifically, when the luminescent material in the reaction cup 1 is sufficiently mixed with the sample to be detected, the central control module 7 controls the second reaction chamber 4 to add sufficient Ej exciting liquid into the reaction cup 1, the chemiluminescent material absorbs immune reaction and then emits light, the photoelectric receiver 5 receives a light-emitting signal and transmits the received light signal to the photomultiplier 6, and the photomultiplier 6 converts the light signal into an electric signal q and amplifies the electric signal q;
the central control module 7 is provided with signal amplification matrix groups M0 and M0(M1, M2 and M3), wherein M1 is a first type sample signal amplification matrix, M2 is a second type sample signal amplification matrix, and M3 is a third type sample signal amplification matrix;
for the ith type sample signal amplification matrix Mi, i is 1,2,3,4, Mi (Mi1, Mi2, Mi3, Mi4), where Mi1 is the first preset signal amplification parameter of the ith type sample, Mi2 is the second preset signal amplification parameter of the ith type sample, Mi3 is the third preset signal amplification parameter of the ith type sample, and Mi4 is the fourth preset signal amplification parameter of the ith type sample;
when the jth item of detection is performed on the ith type of sample, the central control module 7 selects Mij from the M0 matrix group as an electric signal amplification parameter, and the central control module 7 amplifies the electric signal to Q, where Q is Q × Mij.
Specifically, when the electrical signal Q' meets the data processing requirement, the central control module 7 performs data processing on the telecommunication to generate a detection result and displays the detection result through the display screen 8.
Specifically, the detection technology platform can detect a plurality of samples at one time, and the total number of the samples is not more than 200.
Specifically, the central control module 7 is provided with operation end software, and the software functions include: user login, password change, role assignment authority, real-time display of the state of the technical platform, import and confirmation of the reagent configuration file, and after the detection result is generated, the software can automatically generate the standard song according to the result.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.

Claims (9)

1. A full-automatic chemiluminescence immunoassay technical platform is characterized by comprising:
the reaction cup is used for containing the biological sample to be detected;
the first reaction bin is used for selecting corresponding reaction types according to the types of the samples to expose macromolecules of the biological sample and carrying out qualitative or quantitative analysis on the macromolecules;
the second reaction bin is connected with the first reaction bin through a conveyor belt and is used for carrying out magnetic bead coupling on macromolecules in a biological sample, adding a chemiluminescence substance and excitation liquid and detecting a reaction result;
the sample detector is arranged above the first reaction bin and is used for detecting the type of a sample;
the photoelectric receiver is arranged above the second reaction chamber and is used for collecting a luminous signal of the biological sample in the second reaction chamber;
the photomultiplier is connected with the photoelectric receiver and is used for converting an optical signal of the photoelectric receiver into an electric signal and amplifying the electric signal;
the central control module is respectively connected with the sample type detector, the first reaction bin, the second reaction bin, the photoelectric receiver and the photomultiplier and is used for adjusting the working state of each part;
the display screen is connected with the central control module and is used for displaying the working state of each part and the sample detection result;
the control panel is connected with the central control module and used for manually selecting and adjusting the working state of each part;
a sample species matrix A0, an exposed macromolecule reaction mode matrix B0, a to-be-detected item magnetic bead addition quantity matrix C0, a chemiluminescence substance addition quantity matrix D0 and an excitation liquid species matrix E0 are arranged in the central control module;
when the detection platform detects a sample to be detected, the sample is placed into a reaction cup, the reaction cup is placed into a first reaction chamber, the sample detector detects the type of the sample and transmits the detection result to a central control module, the central control module selects a corresponding macromolecule exposure reaction mode according to the detection result, the first reaction chamber exposes macromolecules in the sample and measures the macromolecule content value F of the sample, the central control module controls the conveyor belt to move the reaction cup from the first reaction chamber to a second reaction chamber, an operator selects detection items through the control module, and the central control module respectively selects corresponding preset magnetic bead addition quantity Cij, preset luminescent material addition quantity Dij and excitation liquid type Ej according to the detection items and adjusts the actual magnetic bead addition quantity value through F;
when the magnetic beads which are measured as Cij 'are added into the reaction cup and coupled for a preset time length T through the magnetic beads, the second reaction bin detects the coupling completion degree G and transmits the detection result to the central control module, and the central control module calculates the coupling compensation time length T' according to the completion degree G;
when the magnetic bead coupling reaction is complete, the central control module adjusts the adding amount of a preset luminescent substance to Dij 'according to the adding amount Cij' of the magnetic beads, the luminescent substance and a sample to be detected are fully mixed, the second reaction chamber adds sufficient Ej exciting liquid into the reaction cup to enable the sample to be detected to react and emit light, the photoelectric receiver receives a luminescent signal and transmits the received optical signal to the photoelectric multiplier tube, the photoelectric multiplier tube converts the optical signal into an electric signal Q and amplifies the electric signal, and the amplified electric signal is Q.
2. The full-automatic chemiluminescence immunoassay platform of claim 1, wherein the central control module further comprises an electrical signal value matrix Q0 and an electrical signal secondary adjustment parameter matrix m0,
for the matrix of electrical signal values Q0, Q0(Q1, Q2), where Q1 is the first preset electrical signal value, Q2 is the second preset electrical signal value, Q1 < Q2;
for the electrical signal secondary adjustment parameter matrix m0, m0(m1, m2), wherein m1 is a first preset electrical signal secondary adjustment parameter, and m2 is a second preset electrical signal secondary adjustment parameter;
the central control module compares the Q and the QO matrix internal parameters and adjusts the electric signal Q according to the comparison result:
when Q is not more than Q1, the central control module judges that the electric signal Q is too small and does not meet the data processing requirement, the central control module selects m1 from the m0 matrix as the secondary adjustment parameter of the electric signal and adjusts the electric signal to Q', Q ═ Q × m 1;
when Q is more than Q1 and less than or equal to Q2, the central control module judges that the electric signal Q meets the data processing requirement;
when Q is more than Q1, the central control module judges that the electric signal Q is too large and does not meet the data processing requirement, the central control module selects m2 from the m0 matrix as the secondary adjustment parameter of the electric signal and adjusts the electric signal into Q',
Figure FDA0002767551160000021
when the central control module adjusts the electric signal to be Q ', the central control module compares the Q' with the internal parameters of the QO matrix, and when Q1 is more than Q 'and less than or equal to Q2, the central control module judges that the electric signal Q' meets the data processing requirements; when Q 'is not in the range of Q1-Q2, the operation is repeated until Q1 is less than Q' ≦ Q2.
3. The full-automatic chemiluminescence immunoassay platform of claim 1, wherein the bead addition matrix groups for items to be detected are C0 and C0(C1, C2 and C3), wherein C1 is the bead addition matrix for items to be detected of the first type of sample, C2 is the bead addition matrix for items to be detected of the second type of sample, and C3 is the bead addition matrix for items to be detected of the third type of sample;
for an i-th type sample to-be-detected item magnetic bead addition quantity matrix Ci, Ci (Ci1, Ci2, Ci3 and Ci4), wherein Ci1 is the first detection item magnetic bead addition quantity of the i-th type sample, Ci2 is the second detection item magnetic bead addition quantity of the i-th type sample, Ci3 is the third detection item magnetic bead addition quantity of the i-th type sample, and Ci4 is the fourth detection item magnetic bead addition quantity of the i-th type sample;
the central control module is also provided with a coupling completion degree matrix G0 and a magnetic bead coupling duration compensation parameter matrix t 0;
for the coupling completion degree matrices G0, G0(G1, G2, G3), wherein G1 is the first predetermined coupling completion degree, G2 is the second predetermined coupling completion degree, G3 is the third predetermined coupling completion degree, the completion degree values sequentially increase;
for a magnetic bead coupling duration compensation parameter matrix t0, t0(t1, t2, t3), wherein t1 is a first preset magnetic bead coupling duration compensation parameter, t2 is a second preset magnetic bead coupling duration compensation parameter, t3 is a third preset magnetic bead coupling duration compensation parameter, and the values of the compensation parameters are sequentially reduced;
the central control module adds Cij' measuring magnetic beads into the reaction cup, when the preset time length T is coupled through the magnetic beads, the second reaction bin detects the coupling completion degree G and transmits the detection result to the central control module, and the central control module compares the G with the internal parameters of the G0 matrix:
when G is less than or equal to G1, the central control module judges that the coupling of the magnetic beads is incomplete and selects t1 from the t0 matrix as a coupling duration compensation parameter;
when G is more than G1 and less than or equal to G2, the central control module judges that the coupling of the magnetic beads is incomplete and selects t2 from a t0 matrix as a coupling duration compensation parameter;
when G is more than G2 and less than or equal to G3, the central control module judges that the coupling of the magnetic beads is incomplete and selects t3 from a t0 matrix as a coupling duration compensation parameter;
when G is larger than G3, the central control module judges that the magnetic beads are completely coupled;
when the central control module judges that the coupling of the magnetic beads is incomplete and selects tp as a coupling time length compensation parameter, p is 1,2 and 3, and the central control module calculates the coupling compensation time length T ', T' is T multiplied by tp; when the coupling compensation time length T ' passes, the second reaction bin detects the coupling completion degree G ', and the operation is repeated until G ' is more than G3;
when the magnetic bead coupling reaction is complete, the central control module adjusts the adding amount of the preset luminescent substance to Dij 'according to the adding amount of the magnetic bead Cij',
Figure FDA0002767551160000031
wherein L is a compensation parameter for adjusting the addition amount of the magnetic beads to the addition amount of the luminescent material.
4. The full-automatic chemiluminescent immunoassay platform of claim 1, wherein the sample species matrix is a0, a0(a1, a2, A3), wherein a1 is a first predetermined sample species, a2 is a second predetermined sample species, and A3 is a third predetermined sample species;
exposing macromolecular reaction pattern matrixes B0 and B0(B1, B2 and B3), wherein B1 is a first preset reaction pattern, B2 is a second preset reaction pattern, and B3 is a third preset reaction pattern;
the reaction cup filled with the biological sample to be detected is placed into a first detection bin, the sample detector detects the type A of the biological sample in the first reaction bin and transmits a detection result to a central control module, and the central control module compares the A with internal parameters of a matrix A0:
when A is A1, the central control module selects B1 from the matrix B0 as an exposure macromolecule reaction mode;
when A is A2, the central control module selects B2 from the matrix B0 as an exposure macromolecule reaction mode;
when A is A3, the central control module selects B3 from the matrix B0 as an exposure macromolecule reaction mode;
when the macromolecules of the biological sample are fully exposed, the first reaction chamber quantifies the macromolecules, and the determination content value is F.
5. The platform of claim 2, wherein the chemiluminescent additions matrix sets D0, D0(D1, D2, D3), wherein D1 is the first type of sample luminescent material additions matrix, D2 is the second type of sample luminescent material additions matrix, and D3 is the third type of sample luminescent material additions matrix;
for the ith type sample illuminant addition quantity matrix Di, i is 1,2,3, Di (Di1, Di2, Di3, Di4), wherein Di1 is the illuminant addition quantity of the first detection item of the ith type sample, Di2 is the illuminant addition quantity of the second detection item of the ith type sample, Di3 is the illuminant addition quantity of the third detection item of the ith type sample, and Di4 is the illuminant addition quantity of the fourth detection item of the ith type sample;
for the excitation liquid type matrix E0, E0(E1, E2, E3, E4), where E1 is the first type excitation liquid, E2 is the second detection item excitation liquid, E3 is the third detection item excitation liquid, and E4 is the fourth detection item excitation liquid;
the central control module is also provided with a macromolecule content value matrix F0(F1, F2, F3 and F4), wherein F1 is a first preset macromolecule content value, F2 is a second preset macromolecule content value, F3 is a third preset macromolecule content value, F4 is a fourth preset macromolecule content value, and the content values are sequentially increased;
when the jth item of detection is carried out on the ith type sample, j is 1,2,3 and 4, and the central control module selects Cij as the preset magnetic bead addition amount from the magnetic bead addition amount matrix Ci to be detected on the ith type sample; selecting Dij from the Di matrix as a preset illuminant addition amount; selecting Ej from the matrix E0 as the type of the excitation liquid;
the central control module compares the parameters in the F and F0 matrixes to adjust the addition amount of the magnetic beads:
when F is less than or equal to F1, the central control module selects c1 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is more than F1 and less than or equal to F2, the central control module selects c2 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is more than F2 and less than or equal to F3, the central control module does not adjust the addition amount of the magnetic beads;
when F is more than F3 and less than or equal to F4, the central control module selects c3 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when F is larger than F4, the central control module selects c4 from the F0 matrix as a magnetic bead addition amount compensation parameter;
when the central control module selects ck to adjust the addition quantity Cij of the magnetic beads, k is 1,2,3 and 4, and the central control module adjusts the addition quantity of the magnetic beads to Cij 'and Cij' is Cij multiplied by ck.
6. The full-automatic chemiluminescence immunoassay platform of claim 1, wherein when the luminophor in the reaction cup is sufficiently mixed with the sample to be detected, the central control module controls the second reaction chamber to add sufficient Ej exciting liquid into the reaction cup, the chemiluminescence substance absorbs the immunoreaction and then emits light, the photoelectric receiver receives the light-emitting signal and transmits the received light signal to the photomultiplier, and the photomultiplier converts the light signal into an electrical signal q and amplifies the electrical signal q;
the central control module is provided with signal amplification matrix groups M0 and M0(M1, M2 and M3), wherein M1 is a first type sample signal amplification matrix, M2 is a second type sample signal amplification matrix, and M3 is a third type sample signal amplification matrix;
for the ith type sample signal amplification matrix Mi, i is 1,2,3,4, Mi (Mi1, Mi2, Mi3, Mi4), where Mi1 is the first preset signal amplification parameter of the ith type sample, Mi2 is the second preset signal amplification parameter of the ith type sample, Mi3 is the third preset signal amplification parameter of the ith type sample, and Mi4 is the fourth preset signal amplification parameter of the ith type sample;
when the jth item of detection is carried out on the ith type of sample, the central control module selects Mij from the M0 matrix group as an electric signal amplification parameter, and amplifies the electric signal to Q, wherein Q is Q multiplied by Mij.
7. The full-automatic chemiluminescence immunoassay platform of claim 1, wherein when the electrical signal Q' meets the data processing requirement, the central control module performs data processing on the telecommunication to generate a detection result and displays the detection result through a display screen.
8. The full-automatic chemiluminescence immunoassay technical platform of claim 1, wherein the detection technical platform can detect a plurality of samples at one time, and the total number of the samples is not more than 200.
9. The full-automatic chemiluminescence immunoassay technical platform of claim 1, wherein an operation terminal software is arranged in the central control module, and the software functions comprise: user login, password change, role assignment authority, real-time display of the state of the technical platform, import and confirmation of the reagent configuration file, and after the detection result is generated, the software can automatically generate the standard song according to the result.
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王弼陡: "全自动化学发光免疫分析仪测控系统的研究", 《中国博士学位论文全文数据库 信息科技辑》 *

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