CN112430531A - 微流控芯片、精确定量的生物样品微流操作的装置和方法 - Google Patents
微流控芯片、精确定量的生物样品微流操作的装置和方法 Download PDFInfo
- Publication number
- CN112430531A CN112430531A CN202011124470.0A CN202011124470A CN112430531A CN 112430531 A CN112430531 A CN 112430531A CN 202011124470 A CN202011124470 A CN 202011124470A CN 112430531 A CN112430531 A CN 112430531A
- Authority
- CN
- China
- Prior art keywords
- channel
- liquid
- biological sample
- pressure
- microfluidic chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012472 biological sample Substances 0.000 title claims abstract description 152
- 238000000034 method Methods 0.000 title claims abstract description 72
- 239000007788 liquid Substances 0.000 claims abstract description 368
- 230000007246 mechanism Effects 0.000 claims abstract description 123
- 239000012530 fluid Substances 0.000 claims description 88
- 238000007710 freezing Methods 0.000 claims description 76
- 230000008014 freezing Effects 0.000 claims description 75
- 239000000243 solution Substances 0.000 claims description 68
- 239000003085 diluting agent Substances 0.000 claims description 43
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 40
- 238000005406 washing Methods 0.000 claims description 31
- 239000007789 gas Substances 0.000 claims description 24
- 238000004017 vitrification Methods 0.000 claims description 24
- 229910052757 nitrogen Inorganic materials 0.000 claims description 20
- 239000000523 sample Substances 0.000 claims description 20
- 239000012531 culture fluid Substances 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 14
- 239000011259 mixed solution Substances 0.000 claims description 10
- 239000012298 atmosphere Substances 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 230000004044 response Effects 0.000 claims description 4
- 230000005484 gravity Effects 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000010257 thawing Methods 0.000 abstract description 34
- 230000008569 process Effects 0.000 abstract description 24
- 230000000694 effects Effects 0.000 abstract description 10
- 239000012895 dilution Substances 0.000 abstract description 4
- 238000010790 dilution Methods 0.000 abstract description 4
- 238000011002 quantification Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 285
- 238000013461 design Methods 0.000 description 33
- 238000010586 diagram Methods 0.000 description 28
- 230000001276 controlling effect Effects 0.000 description 21
- 238000004891 communication Methods 0.000 description 14
- 238000001125 extrusion Methods 0.000 description 11
- 230000008859 change Effects 0.000 description 8
- 238000004113 cell culture Methods 0.000 description 6
- 238000005138 cryopreservation Methods 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 230000009471 action Effects 0.000 description 5
- 239000013078 crystal Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000011067 equilibration Methods 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 229920000089 Cyclic olefin copolymer Polymers 0.000 description 4
- 239000004713 Cyclic olefin copolymer Substances 0.000 description 4
- 239000012930 cell culture fluid Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000003825 pressing Methods 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000008358 core component Substances 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 239000012809 cooling fluid Substances 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 238000003754 machining Methods 0.000 description 2
- 210000000287 oocyte Anatomy 0.000 description 2
- 238000001259 photo etching Methods 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 231100000045 chemical toxicity Toxicity 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000005459 micromachining Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Dispersion Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (31)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011124470.0A CN112430531B (zh) | 2020-10-20 | 2020-10-20 | 微流控芯片、精确定量的生物样品微流操作的装置和方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011124470.0A CN112430531B (zh) | 2020-10-20 | 2020-10-20 | 微流控芯片、精确定量的生物样品微流操作的装置和方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN112430531A true CN112430531A (zh) | 2021-03-02 |
CN112430531B CN112430531B (zh) | 2022-03-04 |
Family
ID=74695730
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202011124470.0A Active CN112430531B (zh) | 2020-10-20 | 2020-10-20 | 微流控芯片、精确定量的生物样品微流操作的装置和方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112430531B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116849914A (zh) * | 2023-07-13 | 2023-10-10 | 中国科学院深圳先进技术研究院 | 一种可重复使用的拉普拉斯阀及青光眼引流装置 |
WO2024108537A1 (zh) * | 2022-11-25 | 2024-05-30 | 中国科学院深圳先进技术研究院 | 一种数字定量体积测量的生化反应方法及系统 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101664704A (zh) * | 2009-09-28 | 2010-03-10 | 赖大邃 | 细胞微量吸取贮存器 |
CN103451090A (zh) * | 2013-09-18 | 2013-12-18 | 上海理工大学 | 微流体细胞处理芯片及其应用方法 |
CN203446410U (zh) * | 2013-05-28 | 2014-02-26 | 武汉大学 | 用于超快速冷冻精子的微流控芯片 |
WO2015057641A1 (en) * | 2013-10-15 | 2015-04-23 | The Regents Of The University Of Michigan | Vitrification of biological material |
CN104745528A (zh) * | 2015-03-13 | 2015-07-01 | 深圳普若赛斯生物科技有限公司 | 一种卵母细胞或胚胎冻存复苏的方法及其使用的复苏液 |
WO2018148756A1 (en) * | 2017-02-13 | 2018-08-16 | The Regents Of The University Of California | Apparatus and methods for digital droplet flowmetry |
CN109628297A (zh) * | 2018-12-14 | 2019-04-16 | 浙江大学宁波理工学院 | 一种微流控高通量生物样品滴冻保存装置 |
-
2020
- 2020-10-20 CN CN202011124470.0A patent/CN112430531B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101664704A (zh) * | 2009-09-28 | 2010-03-10 | 赖大邃 | 细胞微量吸取贮存器 |
CN203446410U (zh) * | 2013-05-28 | 2014-02-26 | 武汉大学 | 用于超快速冷冻精子的微流控芯片 |
CN103451090A (zh) * | 2013-09-18 | 2013-12-18 | 上海理工大学 | 微流体细胞处理芯片及其应用方法 |
WO2015057641A1 (en) * | 2013-10-15 | 2015-04-23 | The Regents Of The University Of Michigan | Vitrification of biological material |
CN104745528A (zh) * | 2015-03-13 | 2015-07-01 | 深圳普若赛斯生物科技有限公司 | 一种卵母细胞或胚胎冻存复苏的方法及其使用的复苏液 |
WO2018148756A1 (en) * | 2017-02-13 | 2018-08-16 | The Regents Of The University Of California | Apparatus and methods for digital droplet flowmetry |
CN109628297A (zh) * | 2018-12-14 | 2019-04-16 | 浙江大学宁波理工学院 | 一种微流控高通量生物样品滴冻保存装置 |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024108537A1 (zh) * | 2022-11-25 | 2024-05-30 | 中国科学院深圳先进技术研究院 | 一种数字定量体积测量的生化反应方法及系统 |
CN116849914A (zh) * | 2023-07-13 | 2023-10-10 | 中国科学院深圳先进技术研究院 | 一种可重复使用的拉普拉斯阀及青光眼引流装置 |
CN116849914B (zh) * | 2023-07-13 | 2024-05-17 | 中国科学院深圳先进技术研究院 | 一种可重复使用的拉普拉斯阀及青光眼引流装置 |
Also Published As
Publication number | Publication date |
---|---|
CN112430531B (zh) | 2022-03-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112430531B (zh) | 微流控芯片、精确定量的生物样品微流操作的装置和方法 | |
JP7003086B2 (ja) | 細胞培養装置相互接続および流体装置相互接続のためのシステムおよび方法 | |
US20190133114A1 (en) | Microfluidic Embryo and Gamete Culture Systems | |
CN105831105B (zh) | 微流体细胞处理芯片及其应用方法 | |
US9943848B2 (en) | Apparatus and method for cell disruption | |
US6881541B2 (en) | Method for analyzing a fluid sample | |
KR102615574B1 (ko) | 샘플을 준비하기 위한 유리화 장치 및 방법 | |
US8268603B2 (en) | Apparatus and method for cell disruption | |
CN219463447U (zh) | 一种可复用的数字式液滴生成装置及玻璃化冷冻系统 | |
CN219522120U (zh) | 用于实现自动化微流操作的机械臂接口组件及玻璃化冷冻系统 | |
CN115920988A (zh) | 一种可复用的数字式液滴生成装置及玻璃化冷冻系统 | |
CN220004073U (zh) | 一种可控开放式的微流控玻璃化冷冻芯片 | |
CN116352679A (zh) | 用于实现自动化微流操作的机械臂接口组件及玻璃化冷冻系统 | |
CN219540337U (zh) | 一种适合批量生产的微流控芯片 | |
RU2781072C2 (ru) | Устройство для витрификации и способ получения образца | |
Neculai-Valeanu et al. | Improving the outcome of in vitro embryo production through microfluidic technology |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20210820 Address after: 215124 904, block B, Huihu building, No. 10, Yueliangwan Road, Suzhou Industrial Park, Suzhou, Jiangsu Province Applicant after: Suzhou Mochang Technology Co.,Ltd. Address before: 518055 building 31, area B, jiyuecheng, 113 langtangxing Road, xilitang, Nanshan, Shenzhen, Guangdong Applicant before: Fang Zecong |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240723 Address after: Room A, Room 1106, No. 818 Longhua East Road, Huangpu District, Shanghai 200001 Patentee after: Prospect Equity Investment Management (Shanghai) Co.,Ltd. Country or region after: China Address before: 215124 904, block B, Huihu building, No. 10, Yueliangwan Road, Suzhou Industrial Park, Suzhou, Jiangsu Province Patentee before: Suzhou Mochang Technology Co.,Ltd. Country or region before: China |