CN112410258A - Microbial agent for degrading kitchen waste and preparation method thereof - Google Patents
Microbial agent for degrading kitchen waste and preparation method thereof Download PDFInfo
- Publication number
- CN112410258A CN112410258A CN202011374698.5A CN202011374698A CN112410258A CN 112410258 A CN112410258 A CN 112410258A CN 202011374698 A CN202011374698 A CN 202011374698A CN 112410258 A CN112410258 A CN 112410258A
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- Prior art keywords
- degrading
- kitchen waste
- microbial agent
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- 230000000593 degrading effect Effects 0.000 title claims abstract description 35
- 239000010806 kitchen waste Substances 0.000 title claims abstract description 22
- 230000000813 microbial effect Effects 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- 239000002131 composite material Substances 0.000 claims abstract description 16
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 241001052560 Thallis Species 0.000 claims abstract description 10
- 239000012876 carrier material Substances 0.000 claims abstract description 10
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 8
- 229920002472 Starch Polymers 0.000 claims abstract description 7
- 235000019698 starch Nutrition 0.000 claims abstract description 7
- 239000008107 starch Substances 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 241000209140 Triticum Species 0.000 claims abstract description 5
- 235000021307 Triticum Nutrition 0.000 claims abstract description 5
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 229920002678 cellulose Polymers 0.000 claims abstract description 4
- 239000001913 cellulose Substances 0.000 claims abstract description 4
- 235000018102 proteins Nutrition 0.000 description 7
- 230000015556 catabolic process Effects 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 235000019197 fats Nutrition 0.000 description 4
- 235000019737 Animal fat Nutrition 0.000 description 3
- 235000013325 dietary fiber Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 206010004542 Bezoar Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000605896 Fibrobacter succinogenes Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000192031 Ruminococcus Species 0.000 description 1
- 241000235015 Yarrowia lipolytica Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000010813 municipal solid waste Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000011265 semifinished product Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Processing Of Solid Wastes (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the technical field of kitchen waste treatment, in particular to a microbial agent for degrading kitchen waste and a preparation method thereof, wherein the microbial agent comprises protein degrading bacteria accounting for 30% of the total weight, starch degrading bacteria accounting for 15% of the total weight, fat degrading bacteria accounting for 35% of the total weight, and cellulose degrading bacteria accounting for 20% of the total weight. The invention also provides a preparation method of the microbial agent for degrading the kitchen waste, which comprises the following steps: firstly, culturing strains of each raw material independently, centrifugally collecting, and mixing obtained thalli in proportion to prepare composite thalli; secondly, mixing the wheat hulls and the sawdust according to a volume ratio of 1:4 to prepare a carrier material; and step three, mixing the composite bacteria obtained in the step one with the carrier material obtained in the step two to obtain the composite microbial inoculum.
Description
Technical Field
The invention relates to the technical field of kitchen waste treatment, in particular to a microbial agent for degrading kitchen waste and a preparation method thereof.
Background
In the urban domestic garbage structure of China, the proportion of kitchen organic matters is 30% -50%, and the proportion is large. The main components of the kitchen organic matter comprise organic substances such as protein, starch, dietary fiber, animal fat and the like. The organic fertilizer has the characteristics of high water content, high organic matter proportion, high oil content, high salinity and the like, is easy to rot and ferment, can breed bacteria, and has the dual characteristics of typical resources and wastes. At present, the main treatment modes of the kitchen organic matters are incineration, landfill, feed conversion and biological treatment technologies. The feed and biological treatment technology is a mainstream process for treating the kitchen organic matters in a standardized manner at the present stage. In recent years, several applicable technologies are introduced and developed domestically, mainly including biological fermentation, composting, anaerobic biogas production, subcritical hydrolysis technology and the like. Adverse effects of kitchen organic matters are gradually attracting attention of various cities in China, and how to realize reduction, harmlessness and recycling of the kitchen organic matters is an urgent problem facing China.
Disclosure of Invention
The invention provides a microbial agent for degrading kitchen wastes and a preparation method thereof, and solves the problem of the current degradation treatment of the kitchen wastes.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a microbial agent for degrading kitchen wastes comprises 30% by weight of protein degrading bacteria, 15% by weight of starch degrading bacteria, 35% by weight of fat degrading bacteria and 20% by weight of cellulose degrading bacteria.
The invention also provides a preparation method of the microbial agent for degrading the kitchen waste, which comprises the following steps:
firstly, culturing strains of each raw material independently, centrifugally collecting, and mixing obtained thalli in proportion to prepare composite thalli;
secondly, mixing the wheat hulls and the sawdust according to a volume ratio of 1:4 to prepare a carrier material;
and step three, mixing the composite bacteria obtained in the step one with the carrier material obtained in the step two to obtain the composite microbial inoculum.
The invention has the beneficial effects that: the invention aims at the characteristics that the main components in the kitchen waste comprise organic substances such as protein, starch, dietary fiber and animal fat, protein degrading bacteria, starch degrading bacteria, fat degrading bacteria and cellulose degrading bacteria are selected in a targeted manner, the protein, starch, dietary fiber and animal fat in the kitchen waste are respectively eliminated, the contents of the protein and fat are higher according to the characteristics of Chinese food, the contents of the protein degrading bacteria and the fat degrading bacteria are improved in the composition proportion of the microbial inoculum in a targeted manner, the degradation of the kitchen waste is effectively promoted, the composite bacteria and a fluffy and breathable excellent carrier are mixed to prepare the microbial inoculum, the microbial inoculum is used for degrading the kitchen waste, the degradation effect is obvious in a high-temperature operation state, and the degradation rate is between 90 and 95 after 24 hours of degradation. The treatment method is simple, stable in effect and high in efficiency, and degradation products do not pollute the environment; the kitchen degradation product obtained by degrading the kitchen waste is a high organic matter semi-finished product, and can be further utilized in various aspects such as organic fertilizer raw material preparation and the like
Detailed Description
Example 1
A microbial agent for degrading kitchen wastes comprises 300g of crescent moon bacillus, 150g of bacillus subtilis, 350g of COD degrading bacteria and 200g of bezoar ruminococcus.
The invention also provides a preparation method of the microbial agent for degrading the kitchen waste, which comprises the following steps:
firstly, culturing strains of each raw material independently, centrifugally collecting, and mixing obtained thalli in proportion to prepare composite thalli;
secondly, mixing the wheat hulls and the sawdust according to a volume ratio of 1:4 to prepare a carrier material;
and step three, mixing the composite bacteria obtained in the step one with the carrier material obtained in the step two to obtain the composite microbial inoculum.
Example 2
A microbial agent for degrading kitchen wastes comprises 300g of gram-negative bacteria, 150g of Escherichia coli, 350g of Candida lipolytica and 200g of Bacteroides succinogenes.
The invention also provides a preparation method of the microbial agent for degrading the kitchen waste, which comprises the following steps:
firstly, culturing strains of each raw material independently, centrifugally collecting, and mixing obtained thalli in proportion to prepare composite thalli;
secondly, mixing the wheat hulls and the sawdust according to a volume ratio of 1:4 to prepare a carrier material;
and step three, mixing the composite bacteria obtained in the step one with the carrier material obtained in the step two to obtain the composite microbial inoculum.
Claims (2)
1. A microbial agent for degrading kitchen waste is characterized in that: comprises 30 percent of protein degrading bacteria, 15 percent of starch degrading bacteria, 35 percent of fat degrading bacteria and 20 percent of cellulose degrading bacteria.
2. The preparation method of the microbial agent for degrading kitchen waste according to claim 1, characterized by comprising the following steps:
firstly, culturing strains of each raw material independently, centrifugally collecting, and mixing obtained thalli in proportion to prepare composite thalli;
secondly, mixing the wheat hulls and the sawdust according to a volume ratio of 1:4 to prepare a carrier material;
and step three, mixing the composite bacteria obtained in the step one with the carrier material obtained in the step two to obtain the composite microbial inoculum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011374698.5A CN112410258A (en) | 2020-11-30 | 2020-11-30 | Microbial agent for degrading kitchen waste and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011374698.5A CN112410258A (en) | 2020-11-30 | 2020-11-30 | Microbial agent for degrading kitchen waste and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112410258A true CN112410258A (en) | 2021-02-26 |
Family
ID=74828957
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011374698.5A Pending CN112410258A (en) | 2020-11-30 | 2020-11-30 | Microbial agent for degrading kitchen waste and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112410258A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116784422A (en) * | 2023-06-20 | 2023-09-22 | 浙江农林大学 | Method for preparing low-salt organic fertilizer and high-salt protein feed by treating kitchen waste |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948756A (en) * | 2010-09-16 | 2011-01-19 | 江门市汉宇电器有限公司 | High-efficient restaurant-kitchen waste degrading microorganism bacterial agent and preparation method and application thereof |
| CN102586112A (en) * | 2012-03-14 | 2012-07-18 | 田兴军 | Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof |
| CN106282046A (en) * | 2015-06-03 | 2017-01-04 | 南京恩皆优生物科技有限公司 | A kind of microbial bacterial agent for kitchen organic matter degradation |
-
2020
- 2020-11-30 CN CN202011374698.5A patent/CN112410258A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948756A (en) * | 2010-09-16 | 2011-01-19 | 江门市汉宇电器有限公司 | High-efficient restaurant-kitchen waste degrading microorganism bacterial agent and preparation method and application thereof |
| CN102586112A (en) * | 2012-03-14 | 2012-07-18 | 田兴军 | Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof |
| CN106282046A (en) * | 2015-06-03 | 2017-01-04 | 南京恩皆优生物科技有限公司 | A kind of microbial bacterial agent for kitchen organic matter degradation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116784422A (en) * | 2023-06-20 | 2023-09-22 | 浙江农林大学 | Method for preparing low-salt organic fertilizer and high-salt protein feed by treating kitchen waste |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210226 |
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| RJ01 | Rejection of invention patent application after publication |