CN112409481A - 抗p40蛋白单克隆抗体、细胞系及其制备方法和应用 - Google Patents
抗p40蛋白单克隆抗体、细胞系及其制备方法和应用 Download PDFInfo
- Publication number
- CN112409481A CN112409481A CN202011392419.8A CN202011392419A CN112409481A CN 112409481 A CN112409481 A CN 112409481A CN 202011392419 A CN202011392419 A CN 202011392419A CN 112409481 A CN112409481 A CN 112409481A
- Authority
- CN
- China
- Prior art keywords
- monoclonal antibody
- protein
- cell line
- amino acid
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 28
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 101800000857 p40 protein Proteins 0.000 claims abstract description 26
- 210000004408 hybridoma Anatomy 0.000 claims abstract description 16
- 150000001413 amino acids Chemical class 0.000 claims abstract description 14
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 230000002163 immunogen Effects 0.000 claims abstract description 5
- 230000003248 secreting effect Effects 0.000 claims abstract description 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 7
- 238000002965 ELISA Methods 0.000 claims description 7
- 238000003018 immunoassay Methods 0.000 claims description 4
- 102000014914 Carrier Proteins Human genes 0.000 claims description 3
- 108010078791 Carrier Proteins Proteins 0.000 claims description 3
- 238000003364 immunohistochemistry Methods 0.000 claims description 3
- 238000009629 microbiological culture Methods 0.000 claims description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 238000003119 immunoblot Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 34
- 238000001514 detection method Methods 0.000 abstract description 13
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 230000001900 immune effect Effects 0.000 abstract description 2
- 238000013115 immunohistochemical detection Methods 0.000 abstract description 2
- 101000694288 Homo sapiens 40S ribosomal protein SA Proteins 0.000 abstract 1
- 101001090483 Homo sapiens Glutathione S-transferase LANCL1 Proteins 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 16
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 12
- 239000000523 sample Substances 0.000 description 10
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 201000005249 lung adenocarcinoma Diseases 0.000 description 9
- 238000010186 staining Methods 0.000 description 9
- 206010041823 squamous cell carcinoma Diseases 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- 210000004072 lung Anatomy 0.000 description 7
- 201000005243 lung squamous cell carcinoma Diseases 0.000 description 7
- 102100028629 Cytoskeleton-associated protein 4 Human genes 0.000 description 6
- 241000283973 Oryctolagus cuniculus Species 0.000 description 6
- 238000011532 immunohistochemical staining Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- 239000001993 wax Substances 0.000 description 6
- 101710087047 Cytoskeleton-associated protein 4 Proteins 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 101710140697 Tumor protein 63 Proteins 0.000 description 5
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000003053 immunization Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 3
- 230000000890 antigenic effect Effects 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000012228 culture supernatant Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000003748 differential diagnosis Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 201000005296 lung carcinoma Diseases 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 108010086434 alanyl-seryl-glycine Proteins 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 230000007910 cell fusion Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000003113 dilution method Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 108010051242 phenylalanylserine Proteins 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000005758 transcription activity Effects 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- NVKAWKQGWWIWPM-ABEVXSGRSA-N 17-β-hydroxy-5-α-Androstan-3-one Chemical compound C1C(=O)CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CC[C@H]21 NVKAWKQGWWIWPM-ABEVXSGRSA-N 0.000 description 1
- RTZCUEHYUQZIDE-WHFBIAKZSA-N Ala-Ser-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O RTZCUEHYUQZIDE-WHFBIAKZSA-N 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- PDQBXRSOSCTGKY-ACZMJKKPSA-N Asn-Ala-Gln Chemical compound C[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N PDQBXRSOSCTGKY-ACZMJKKPSA-N 0.000 description 1
- QUMKPKWYDVMGNT-NUMRIWBASA-N Asn-Thr-Gln Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N)O QUMKPKWYDVMGNT-NUMRIWBASA-N 0.000 description 1
- RDLYUKRPEJERMM-XIRDDKMYSA-N Asn-Trp-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC(C)C)C(O)=O RDLYUKRPEJERMM-XIRDDKMYSA-N 0.000 description 1
- MRQQMVZUHXUPEV-IHRRRGAJSA-N Asp-Arg-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O MRQQMVZUHXUPEV-IHRRRGAJSA-N 0.000 description 1
- PZXPWHFYZXTFBI-YUMQZZPRSA-N Asp-Gly-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CC(O)=O PZXPWHFYZXTFBI-YUMQZZPRSA-N 0.000 description 1
- AYFVRYXNDHBECD-YUMQZZPRSA-N Asp-Leu-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O AYFVRYXNDHBECD-YUMQZZPRSA-N 0.000 description 1
- MNQMTYSEKZHIDF-GCJQMDKQSA-N Asp-Thr-Ala Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O MNQMTYSEKZHIDF-GCJQMDKQSA-N 0.000 description 1
- ZUNMTUPRQMWMHX-LSJOCFKGSA-N Asp-Val-Val Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(O)=O ZUNMTUPRQMWMHX-LSJOCFKGSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- KEBJBKIASQVRJS-WDSKDSINSA-N Cys-Gln-Gly Chemical compound C(CC(=O)N)[C@@H](C(=O)NCC(=O)O)NC(=O)[C@H](CS)N KEBJBKIASQVRJS-WDSKDSINSA-N 0.000 description 1
- NIXHTNJAGGFBAW-CIUDSAMLSA-N Cys-Lys-Ser Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CS)N NIXHTNJAGGFBAW-CIUDSAMLSA-N 0.000 description 1
- 208000000461 Esophageal Neoplasms Diseases 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- JXFLPKSDLDEOQK-JHEQGTHGSA-N Gln-Gly-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CCC(N)=O JXFLPKSDLDEOQK-JHEQGTHGSA-N 0.000 description 1
- GQZDDFRXSDGUNG-YVNDNENWSA-N Gln-Ile-Gln Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(O)=O GQZDDFRXSDGUNG-YVNDNENWSA-N 0.000 description 1
- FNAJNWPDTIXYJN-CIUDSAMLSA-N Gln-Pro-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CCC(N)=O FNAJNWPDTIXYJN-CIUDSAMLSA-N 0.000 description 1
- UQKVUFGUSVYJMQ-IRIUXVKKSA-N Gln-Tyr-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)[C@H](CCC(=O)N)N)O UQKVUFGUSVYJMQ-IRIUXVKKSA-N 0.000 description 1
- LKDIBBOKUAASNP-FXQIFTODSA-N Glu-Ala-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(O)=O LKDIBBOKUAASNP-FXQIFTODSA-N 0.000 description 1
- YLJHCWNDBKKOEB-IHRRRGAJSA-N Glu-Glu-Phe Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O YLJHCWNDBKKOEB-IHRRRGAJSA-N 0.000 description 1
- XTZDZAXYPDISRR-MNXVOIDGSA-N Glu-Ile-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N XTZDZAXYPDISRR-MNXVOIDGSA-N 0.000 description 1
- ZYRXTRTUCAVNBQ-GVXVVHGQSA-N Glu-Val-Lys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N ZYRXTRTUCAVNBQ-GVXVVHGQSA-N 0.000 description 1
- GNPVTZJUUBPZKW-WDSKDSINSA-N Gly-Gln-Ser Chemical compound [H]NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(O)=O GNPVTZJUUBPZKW-WDSKDSINSA-N 0.000 description 1
- VIIBEIQMLJEUJG-LAEOZQHASA-N Gly-Ile-Gln Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(O)=O VIIBEIQMLJEUJG-LAEOZQHASA-N 0.000 description 1
- PDUHNKAFQXQNLH-ZETCQYMHSA-N Gly-Lys-Gly Chemical compound NCCCC[C@H](NC(=O)CN)C(=O)NCC(O)=O PDUHNKAFQXQNLH-ZETCQYMHSA-N 0.000 description 1
- SOEGEPHNZOISMT-BYPYZUCNSA-N Gly-Ser-Gly Chemical compound NCC(=O)N[C@@H](CO)C(=O)NCC(O)=O SOEGEPHNZOISMT-BYPYZUCNSA-N 0.000 description 1
- XHVONGZZVUUORG-WEDXCCLWSA-N Gly-Thr-Lys Chemical compound NCC(=O)N[C@@H]([C@H](O)C)C(=O)N[C@H](C(O)=O)CCCCN XHVONGZZVUUORG-WEDXCCLWSA-N 0.000 description 1
- WTUSRDZLLWGYAT-KCTSRDHCSA-N Gly-Trp-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CNC2=CC=CC=C21)NC(=O)CN WTUSRDZLLWGYAT-KCTSRDHCSA-N 0.000 description 1
- GBYYQVBXFVDJPJ-WLTAIBSBSA-N Gly-Tyr-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)CN)O GBYYQVBXFVDJPJ-WLTAIBSBSA-N 0.000 description 1
- YGHSQRJSHKYUJY-SCZZXKLOSA-N Gly-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)CN YGHSQRJSHKYUJY-SCZZXKLOSA-N 0.000 description 1
- 241001272567 Hominoidea Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 101000766853 Homo sapiens Cytoskeleton-associated protein 4 Proteins 0.000 description 1
- 101000987003 Homo sapiens Tumor protein 63 Proteins 0.000 description 1
- ZDNORQNHCJUVOV-KBIXCLLPSA-N Ile-Gln-Ala Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(O)=O ZDNORQNHCJUVOV-KBIXCLLPSA-N 0.000 description 1
- FBGXMKUWQFPHFB-JBDRJPRFSA-N Ile-Ser-Cys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)O)N FBGXMKUWQFPHFB-JBDRJPRFSA-N 0.000 description 1
- NXRNRBOKDBIVKQ-CXTHYWKRSA-N Ile-Tyr-Tyr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC2=CC=C(C=C2)O)C(=O)O)N NXRNRBOKDBIVKQ-CXTHYWKRSA-N 0.000 description 1
- FADYJNXDPBKVCA-UHFFFAOYSA-N L-Phenylalanyl-L-lysin Natural products NCCCCC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 FADYJNXDPBKVCA-UHFFFAOYSA-N 0.000 description 1
- 241000880493 Leptailurus serval Species 0.000 description 1
- VPKIQULSKFVCSM-SRVKXCTJSA-N Leu-Gln-Arg Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O VPKIQULSKFVCSM-SRVKXCTJSA-N 0.000 description 1
- RSFGIMMPWAXNML-MNXVOIDGSA-N Leu-Gln-Ile Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O RSFGIMMPWAXNML-MNXVOIDGSA-N 0.000 description 1
- YVKSMSDXKMSIRX-GUBZILKMSA-N Leu-Glu-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O YVKSMSDXKMSIRX-GUBZILKMSA-N 0.000 description 1
- NRFGTHFONZYFNY-MGHWNKPDSA-N Leu-Ile-Tyr Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 NRFGTHFONZYFNY-MGHWNKPDSA-N 0.000 description 1
- UBZGNBKMIJHOHL-BZSNNMDCSA-N Leu-Leu-Phe Chemical compound CC(C)C[C@H]([NH3+])C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C([O-])=O)CC1=CC=CC=C1 UBZGNBKMIJHOHL-BZSNNMDCSA-N 0.000 description 1
- FKQPWMZLIIATBA-AJNGGQMLSA-N Leu-Lys-Ile Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O FKQPWMZLIIATBA-AJNGGQMLSA-N 0.000 description 1
- CPONGMJGVIAWEH-DCAQKATOSA-N Leu-Met-Ala Chemical compound CSCC[C@H](NC(=O)[C@@H](N)CC(C)C)C(=O)N[C@@H](C)C(O)=O CPONGMJGVIAWEH-DCAQKATOSA-N 0.000 description 1
- YWFZWQKWNDOWPA-XIRDDKMYSA-N Leu-Trp-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC(N)=O)C(O)=O YWFZWQKWNDOWPA-XIRDDKMYSA-N 0.000 description 1
- VUBIPAHVHMZHCM-KKUMJFAQSA-N Leu-Tyr-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CO)C(O)=O)CC1=CC=C(O)C=C1 VUBIPAHVHMZHCM-KKUMJFAQSA-N 0.000 description 1
- MVJRBCJCRYGCKV-GVXVVHGQSA-N Leu-Val-Gln Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O MVJRBCJCRYGCKV-GVXVVHGQSA-N 0.000 description 1
- VKVDRTGWLVZJOM-DCAQKATOSA-N Leu-Val-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O VKVDRTGWLVZJOM-DCAQKATOSA-N 0.000 description 1
- UWKNTTJNVSYXPC-CIUDSAMLSA-N Lys-Ala-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN UWKNTTJNVSYXPC-CIUDSAMLSA-N 0.000 description 1
- QYOXSYXPHUHOJR-GUBZILKMSA-N Lys-Asn-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O QYOXSYXPHUHOJR-GUBZILKMSA-N 0.000 description 1
- GQZMPWBZQALKJO-UWVGGRQHSA-N Lys-Gly-Arg Chemical compound [H]N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O GQZMPWBZQALKJO-UWVGGRQHSA-N 0.000 description 1
- MUXNCRWTWBMNHX-SRVKXCTJSA-N Lys-Leu-Asp Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O MUXNCRWTWBMNHX-SRVKXCTJSA-N 0.000 description 1
- ZJSXCIMWLPSTMG-HSCHXYMDSA-N Lys-Trp-Ile Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O ZJSXCIMWLPSTMG-HSCHXYMDSA-N 0.000 description 1
- YAWKHFKCNSXYDS-XIRDDKMYSA-N Met-Glu-Trp Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)N YAWKHFKCNSXYDS-XIRDDKMYSA-N 0.000 description 1
- OBPCXINRFKHSRY-SDDRHHMPSA-N Met-Met-Pro Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCSC)C(=O)N1CCC[C@@H]1C(=O)O)N OBPCXINRFKHSRY-SDDRHHMPSA-N 0.000 description 1
- SPSSJSICDYYTQN-HJGDQZAQSA-N Met-Thr-Gln Chemical compound CSCC[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CCC(N)=O SPSSJSICDYYTQN-HJGDQZAQSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- AUEJLPRZGVVDNU-UHFFFAOYSA-N N-L-tyrosyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CC1=CC=C(O)C=C1 AUEJLPRZGVVDNU-UHFFFAOYSA-N 0.000 description 1
- KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- LBSARGIQACMGDF-WBAXXEDZSA-N Phe-Ala-Phe Chemical compound C([C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 LBSARGIQACMGDF-WBAXXEDZSA-N 0.000 description 1
- NAXPHWZXEXNDIW-JTQLQIEISA-N Phe-Gly-Gly Chemical compound OC(=O)CNC(=O)CNC(=O)[C@@H](N)CC1=CC=CC=C1 NAXPHWZXEXNDIW-JTQLQIEISA-N 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- VYWNORHENYEQDW-YUMQZZPRSA-N Pro-Gly-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H]1CCCN1 VYWNORHENYEQDW-YUMQZZPRSA-N 0.000 description 1
- FKYKZHOKDOPHSA-DCAQKATOSA-N Pro-Leu-Ser Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O FKYKZHOKDOPHSA-DCAQKATOSA-N 0.000 description 1
- WCNVGGZRTNHOOS-ULQDDVLXSA-N Pro-Lys-Tyr Chemical compound C1C[C@H](NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC2=CC=C(C=C2)O)C(=O)O WCNVGGZRTNHOOS-ULQDDVLXSA-N 0.000 description 1
- BARPGRUZBKFJMA-SRVKXCTJSA-N Pro-Met-Arg Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)NC(=O)[C@@H]1CCCN1 BARPGRUZBKFJMA-SRVKXCTJSA-N 0.000 description 1
- VBZXFFYOBDLLFE-HSHDSVGOSA-N Pro-Trp-Thr Chemical compound N([C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H]([C@H](O)C)C(O)=O)C(=O)[C@@H]1CCCN1 VBZXFFYOBDLLFE-HSHDSVGOSA-N 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- HBOABDXGTMMDSE-GUBZILKMSA-N Ser-Arg-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(O)=O HBOABDXGTMMDSE-GUBZILKMSA-N 0.000 description 1
- FIDMVVBUOCMMJG-CIUDSAMLSA-N Ser-Asn-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CO FIDMVVBUOCMMJG-CIUDSAMLSA-N 0.000 description 1
- FMDHKPRACUXATF-ACZMJKKPSA-N Ser-Gln-Ser Chemical compound OC[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(O)=O FMDHKPRACUXATF-ACZMJKKPSA-N 0.000 description 1
- UFKPDBLKLOBMRH-XHNCKOQMSA-N Ser-Glu-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CO)N)C(=O)O UFKPDBLKLOBMRH-XHNCKOQMSA-N 0.000 description 1
- KDGARKCAKHBEDB-NKWVEPMBSA-N Ser-Gly-Pro Chemical compound C1C[C@@H](N(C1)C(=O)CNC(=O)[C@H](CO)N)C(=O)O KDGARKCAKHBEDB-NKWVEPMBSA-N 0.000 description 1
- XXXAXOWMBOKTRN-XPUUQOCRSA-N Ser-Gly-Val Chemical compound [H]N[C@@H](CO)C(=O)NCC(=O)N[C@@H](C(C)C)C(O)=O XXXAXOWMBOKTRN-XPUUQOCRSA-N 0.000 description 1
- UIPXCLNLUUAMJU-JBDRJPRFSA-N Ser-Ile-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(O)=O UIPXCLNLUUAMJU-JBDRJPRFSA-N 0.000 description 1
- ZIFYDQAFEMIZII-GUBZILKMSA-N Ser-Leu-Glu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O ZIFYDQAFEMIZII-GUBZILKMSA-N 0.000 description 1
- 206010041826 Squamous cell carcinoma of lung Diseases 0.000 description 1
- DGDCHPCRMWEOJR-FQPOAREZSA-N Thr-Ala-Tyr Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 DGDCHPCRMWEOJR-FQPOAREZSA-N 0.000 description 1
- TZKPNGDGUVREEB-FOHZUACHSA-N Thr-Asn-Gly Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O TZKPNGDGUVREEB-FOHZUACHSA-N 0.000 description 1
- KRPKYGOFYUNIGM-XVSYOHENSA-N Thr-Asp-Phe Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N)O KRPKYGOFYUNIGM-XVSYOHENSA-N 0.000 description 1
- XXNLGZRRSKPSGF-HTUGSXCWSA-N Thr-Gln-Phe Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N)O XXNLGZRRSKPSGF-HTUGSXCWSA-N 0.000 description 1
- DJDSEDOKJTZBAR-ZDLURKLDSA-N Thr-Gly-Ser Chemical compound C[C@@H](O)[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O DJDSEDOKJTZBAR-ZDLURKLDSA-N 0.000 description 1
- UDNVOQMPQBEITB-MEYUZBJRSA-N Thr-His-Phe Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O UDNVOQMPQBEITB-MEYUZBJRSA-N 0.000 description 1
- URPSJRMWHQTARR-MBLNEYKQSA-N Thr-Ile-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O URPSJRMWHQTARR-MBLNEYKQSA-N 0.000 description 1
- YOOAQCZYZHGUAZ-KATARQTJSA-N Thr-Leu-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O YOOAQCZYZHGUAZ-KATARQTJSA-N 0.000 description 1
- DEGCBBCMYWNJNA-RHYQMDGZSA-N Thr-Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)[C@@H](C)O DEGCBBCMYWNJNA-RHYQMDGZSA-N 0.000 description 1
- FWTFAZKJORVTIR-VZFHVOOUSA-N Thr-Ser-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O FWTFAZKJORVTIR-VZFHVOOUSA-N 0.000 description 1
- NDZYTIMDOZMECO-SHGPDSBTSA-N Thr-Thr-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O NDZYTIMDOZMECO-SHGPDSBTSA-N 0.000 description 1
- JAWUQFCGNVEDRN-MEYUZBJRSA-N Thr-Tyr-Leu Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC(C)C)C(=O)O)N)O JAWUQFCGNVEDRN-MEYUZBJRSA-N 0.000 description 1
- XGFYGMKZKFRGAI-RCWTZXSCSA-N Thr-Val-Arg Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CCCN=C(N)N XGFYGMKZKFRGAI-RCWTZXSCSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- UUZYQOUJTORBQO-ZVZYQTTQSA-N Trp-Val-Gln Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)=CNC2=C1 UUZYQOUJTORBQO-ZVZYQTTQSA-N 0.000 description 1
- UPODKYBYUBTWSV-BZSNNMDCSA-N Tyr-Phe-Cys Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CS)C(O)=O)C1=CC=C(O)C=C1 UPODKYBYUBTWSV-BZSNNMDCSA-N 0.000 description 1
- NUQZCPSZHGIYTA-HKUYNNGSSA-N Tyr-Trp-Gly Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)NCC(=O)O)NC(=O)[C@H](CC3=CC=C(C=C3)O)N NUQZCPSZHGIYTA-HKUYNNGSSA-N 0.000 description 1
- IZFVRRYRMQFVGX-NRPADANISA-N Val-Ala-Gln Chemical compound C[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](C(C)C)N IZFVRRYRMQFVGX-NRPADANISA-N 0.000 description 1
- HTONZBWRYUKUKC-RCWTZXSCSA-N Val-Thr-Val Chemical compound CC(C)[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O HTONZBWRYUKUKC-RCWTZXSCSA-N 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 108010081404 acein-2 Proteins 0.000 description 1
- 208000009956 adenocarcinoma Diseases 0.000 description 1
- 108010044940 alanylglutamine Proteins 0.000 description 1
- 108010047495 alanylglycine Proteins 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 108010060035 arginylproline Proteins 0.000 description 1
- 210000000270 basal cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 210000001728 clone cell Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 201000004101 esophageal cancer Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 108010063718 gamma-glutamylaspartic acid Proteins 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 108010057083 glutamyl-aspartyl-leucine Proteins 0.000 description 1
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Natural products NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 1
- 108010079413 glycyl-prolyl-glutamic acid Proteins 0.000 description 1
- 108010089804 glycyl-threonine Proteins 0.000 description 1
- 108010010147 glycylglutamine Proteins 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000014829 head and neck neoplasm Diseases 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 108010034529 leucyl-lysine Proteins 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 201000010879 mucinous adenocarcinoma Diseases 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 108010070409 phenylalanyl-glycyl-glycine Proteins 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 108010029020 prolylglycine Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 108010048397 seryl-lysyl-leucine Proteins 0.000 description 1
- 108010026333 seryl-proline Proteins 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 108010080629 tryptophan-leucine Proteins 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57423—Specifically defined cancers of lung
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4703—Regulators; Modulating activity
- G01N2333/4704—Inhibitors; Supressors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
本发明涉及生物检测领域,提供了一种抗p40蛋白单克隆抗体,其重链和轻链可变区的氨基酸序列分别是SEQ ID NO.2和SEQ ID NO.3所示的氨基酸序列。发明人还提供了抗p40蛋白单克隆抗体的制备方法,选取人p40氨基酸第4‑19位蛋白并与KLH偶联,作为免疫原。发明人还提供了一株分泌抗p40蛋白的杂交瘤细胞系,所述细胞系为小鼠杂交瘤细胞系14D5E8F12,保藏号为:CGMCC NO.16208。抗p40蛋白单克隆抗体,具有高特异性、敏感性,可以特异性识别表达p40蛋白的细胞,适用于免疫学检测,特别是免疫组化检测。
Description
技术领域
本发明涉及生物检测领域,特别涉及抗p40蛋白单克隆抗体、细胞系及其制备方法和应用。
背景技术
p40蛋白位于细胞核内,其基因cDNA全长约2.2kb,分子量约40kD,故命名为p40,是由p63基因编码翻译而形成的一种蛋白,无反转录活性,又称△Np63。人体p63基因,位于染色体3q27-29,包含15个外显子,2个独立启动子,其编码的蛋白质分为两大类:(1)从外显子1开始转录生成具有反式激活域(TA)全长蛋白TAp63,通过凋亡和细胞分化促进细胞周期循环;(2)从外显子3开始转录生成不具有反式激活域(TA)的蛋白质亚型p40(△Np63),无反转录活性。其中△Np63(p40)在鳞状细胞癌中是有选择性地表达,p40对鳞癌的敏感性同p63,特异性却优于p63(灵敏度为100%,特异性:p40为98%,p63为60%)。有研究表明,△Np63选择性地表达在肺鳞癌,在所有肺腺癌呈阴性;TAp63不仅限于肺鳞癌,在肺腺癌中的表达率约15%。p63在除外黏液型腺癌的所有亚型腺癌中均表达。p63阳性的低分化肺腺癌可能被错误地诊断为肺鳞癌。
p40被广泛地应用于头颈部肿瘤、食管癌等疾病的诊断。近年将p40应用于肺的研究发现,p40表达于正常支气管粘膜的基底细胞、气管腺体的肌上皮细胞,抗p40抗体在标记鳞状上皮时具有高度特异性。并且p40在肺鳞癌中的特异性表达要胜于P63蛋白。在日常病理诊断中p63被广泛应用于肺癌的分型,在肺鳞癌中有很高的敏感性,但也部分表达肺腺癌,同p36相比而言,p40在肺鳞癌中有着同P36抗体类似的高敏感性,但在肺腺癌中罕见表达。故p40被推荐用于肺鳞癌和肺腺癌的鉴别诊断。
发明内容
发明人提供了一种抗p40蛋白单克隆抗体,所述单克隆抗体重链和轻链可变区的氨基酸序列分别是SEQ ID NO.2和SEQ ID NO.3所示的氨基酸序列。
进一步地,所述单克隆抗体重链和轻链可变区氨基酸序列分别是SEQ ID NO.4和SEQ ID NO.5所示的核苷酸序列所编码。
进一步地,所述单克隆抗体特异性识别p40蛋白。
进一步地,所述单克隆抗体特异性识别中SEQ ID NO.1所示的氨基酸序列。
进一步地,所述单克隆抗体由保藏号为CGMCC NO.16208的杂交瘤细胞系产生。所述细胞株为小鼠杂交瘤细胞系14D5E8F12,分类命名为:小鼠杂交瘤细胞系,该细胞系已于2020年04月24日在中国微生物菌种保藏管理委员会普通微生物中心保藏,地址为北京市朝阳区北辰西路1号院3号中国科学院微生物研究所。
进一步地,所述抗p40蛋白为小鼠IgG1亚型单克隆抗体。
进一步地,发明人还提供了一种抗p40蛋白单克隆抗体的制备方法,选取p40蛋白氨基酸序列第4位至第19位氨基酸并与载体蛋白KLH偶联作为免疫原。
发明人还提供了一株分泌抗p40蛋白的杂交瘤细胞系,所述细胞株为小鼠杂交瘤细胞系14D5E8F12,所述细胞系保藏于中国微生物菌种保藏管理委员会普通微生物中心,保藏日期为2018年7月30日,保藏号为:CGMCC NO.16208。
发明人还提供上述任一所述的抗p40蛋白单克隆抗体,在p40蛋白免疫检测中的用途。
进一步地,所述免疫检测包括免疫组织化学法,免疫印迹法和酶联免疫法。
区别于现有技术,上述技术方案依据p40蛋白与DNA结合的结构、抗原性、组成氨基酸的亲疏水性以及二级结构,选择具有区别于其他类似蛋白、长度适宜且具有特殊抗原性的氨基酸序列第4-19位蛋白(SEQ ID No.1),区域作为抗原肽,并偶联载体KLH,作为免疫原。对小鼠进行免疫,经细胞融合、筛选和亚克隆,获得高效分泌抗p40蛋白单克隆抗体的单克隆细胞系14D5E8F12,以及由该细胞系所分泌的抗p40蛋白单克隆抗体。本方案得到的抗体具有高特异性、敏感性,可以特异性识别表达p40蛋白的细胞,适用于免疫学检测,特别是免疫组化检测。
附图说明
图1:纯化后p40单克隆抗体的聚丙烯酰胺凝胶电泳图。
图2:肺鳞癌免疫组化染色结果图,其中左为(14D5E8F12)的p40,右为市售p40((ZR8)兔单抗)。
图3:肺腺癌免疫组化染色结果图,其中左为(14D5E8F12)的p40,右为市售p40((ZR8)兔单抗)。
具体实施方式
实施例1重组p40蛋白片段的制备
一、基因克隆
从Uniprot数据库(http://www.uniprot.org)中选择编号Q9H3D4的p40蛋白序列作为标准序列。依据其与DNA结合的结构、抗原性、组成氨基酸的亲疏水性以及二级结构,选择具有区别于其他类似蛋白、长度适宜且具有特殊抗原性的区域作为抗原肽。选定p40氨基酸序列第4位至第19位序列,其对应的核苷酸序列为SEQ ID No.1。合成以上p40蛋白片段,在其N端添加巯基修饰并与载体蛋白KLH偶联,提高其免疫原性,作为免疫原备用。
实施例2 14D5E8F12杂交瘤细胞系的建立
一、免疫
将实施例1中获得的p40蛋白-KLH偶联物稀释至1mg/mL,然后与等体积的完全弗氏佐剂(CFA,Sigma公司)混合乳化,对18-20g的Balb/c小鼠(购自福州吴氏实验动物)进行腹部注射免疫,注射剂量为50μg/只。此后每14天加强免疫一次,抗原使用弗氏非完全佐剂(IFA,Sigma公司)乳化,剂量为25μg/只。第2次加强免疫后14天以间接ELISA(波长450nm)检测小鼠血清中抗免疫原的多抗效价,效价最高的小鼠以尾静脉注射冲击免疫,抗原用PBS溶液混匀,剂量为50μg/只。
二、细胞融合
无菌制备免疫达标的小鼠脾细胞悬液,与小鼠骨髓瘤细胞sp2/0(ATCC)以5:1比例混合,1000rpm离心10min,弃上清后,在1分钟内由慢到快加入1mL预热至37℃的PEG(Sigma公司)溶液,加入过程中需轻轻转动离心管,使细胞与PEG充分接触。室温静置90s后,2min内由慢到快加入4mL预热至37℃的无血清DMEM(Hyclone公司)培养基,随后的2min内再加入10mL预热无血清DMEM培养基,最后2min内加完剩余的预热无血清DMEM培养基,定容至50mL,整个加入过程需要缓慢摇动离心管,确保混合均匀,减轻对细胞的伤害。室温静置10min后离心(1000rpm,5min),弃上清,用10-20mLHAT(Sigma公司)培养基重悬细胞,并用HAT培养基稀释至终浓度为0.5×106cells/mL,所有溶液转移至96孔板中,200μL/孔,并做好标记。将96孔培养板小心转移至37℃,5%CO2培养箱中培养。定期检查细胞的生长状态及潜在的污染,注意尽量少开合培养箱,确保培养环境稳定。融合后第5天,向培养板中补加HAT培养基,50μL/孔。
三、ELISA筛选阳性杂交瘤细胞
当融合细胞直径长至大概为1-2mm时,吸取50-200μL培养液上清进行首次细胞筛选(ELISA、IHC-P及其他方法检测),同时往培养孔中补加HAT培养基至200μL。ELISA检测该培养液上清,将检测得到阳性结果的培养孔内细胞培养液全部转移至24孔培养板,补加HT培养基,2mL/孔,培养3天。
重复筛选24孔板中各细胞株,剔除不是阳性结果的培养孔细胞,获得阳性结果较好的培养孔细胞。将这些24孔培养板得到的阳性孔细胞采用有限稀释法进行亚克隆筛选,即将有限稀释法得到细胞液加入96孔培养板中转移至CO2培养箱培养11天,待克隆细胞直径为1-2mm时,重复进行细胞筛选。根据检测结果,每个亚克隆细胞株,选取4个生长良好的单克隆阳性培养孔,并转移至24孔板中继续培养。一段时间后再次筛选24孔板中克隆得到的阳性克隆细胞株,即为分泌特定单克隆抗体的杂交瘤细胞株14D5E8F12。将此细胞株转入T-75培养瓶中扩增至对数生长期保种或进行后续实验。
实施例3体外培养法制备单克隆抗体
一、体外培养
获得稳定的杂交瘤细胞系后,主要采用体外培养法获取单抗。
以含10%胎牛血清的DMEM完全培养基培养杂交瘤细胞,然后低速离心后收集培养上清,4℃储存备用。
二、单克隆抗体的纯化
用rProtein A sepharose Fast Flow(GE公司)亲和层析柱纯化抗体:①装柱,将购买的ProteinA填料适量装于重力层析柱中用平衡缓冲液(0.1M Tris溶液,pH7.0)冲洗至平衡;②上样,将经过0.22μm滤膜过滤的腹水加入装好的层析柱中,控制流速1滴/秒;③平衡,上完样液后使用平衡缓冲液冲洗至平衡;④洗脱,加入洗脱缓冲液(0.1M柠檬酸溶液,pH3.0)冲洗柱子并收集洗脱液;⑤再生,洗脱完成后加入平衡缓冲液冲洗柱子至平衡,2倍柱体积的20%乙醇冲洗后置于4℃保存。最后采用SDS-PAGE法鉴定抗体纯度。结果见图1,纯化后p40,纯度达95%以上,紫外微量分光光度计法测定抗体浓度,浓度达到3.0mg/mL以上。
实施例4单克隆抗体特性鉴定
一、亚型鉴定
将细胞上清稀释成1μg/mL包被酶标板,每孔加100μL,4℃包被过夜,倾空液体,用含0.05%Tween的PBS(PBS-T)洗板3次,每孔加入200μL封闭液(含2%BSA的PBS-T溶液),37℃孵育1h。倾空液体,用PBS-T清洗3次。每孔加入稀释5倍的0.1mL杂交瘤细胞株培养液上清,37℃孵育1h。倾空液体,用PBS-T清洗3次。用封闭液1:400稀释HRP标记的羊抗鼠(κ,λ,IgM,IgG1,IgG2a,IgG2b,IgG3,IgA)抗体(Southern Biotech公司),每孔分别加入0.1mL,37℃孵育1h。倾空液体,用PBS-T清洗3次。每孔加100μLTMB(湖州英创生物科技有限公司)底物(A、B等体积混合溶液)进行显色,室温反应15min,每孔加入50μL 1N HCl溶液终止显色反应,然后酶标仪测定450nm波长下的OD值。结果显示,本发明单克隆抗体为IgG1型鼠源单克隆抗体。
二、亲和常数测定
包被p40蛋白,包被浓度为100μg/ml,100μl/孔,4℃包被过夜,PBS-T洗3次。每孔加200μl封闭液37℃封闭1h,PBS-T洗3次。实施例4中纯化的单克隆抗体,稀释成以下浓度(单位:ng/mL):2000、500、125、62.5、31.25、15.625、3.125、0.625,37℃孵育1h,PBS-T洗3次。HRP标记的羊抗鼠二抗1:5000稀释,每孔100μl,37℃孵育1h,PBS-T洗3次。每孔加入100μlTMB(湖州英创生物科技有限公司)显色液,显色13min,加100μl 1.0N盐溶液终止反应。用酶标仪测定波长450nm的吸光值。画出OD值对应抗体稀释倍数的曲线,找出1/2“平台OD值”对应的抗体浓度A。利用下列公式计算出亲和常数为5.99×109L×mol-1。
实施例6组织芯片染色和鉴定
一、组织蜡块制备过程
对样本组织进行HE切片染色,以确定肿瘤病变部位。对病变位点画圈,预备打孔。制作受体蜡块时,将塑料架置于模具上,将融化的石蜡(熔点在56~58℃)倒入模具,将组织块放入模具中的蜡液中后再加入适量蜡液使组织块完全包埋在蜡液中,冷却至室温后将模具放入-20℃冰箱6min,将蜡块从模具中取出,切片或放入4℃冰箱内保存备用。修片后进行连续切片,厚度定为4μm,将连续切片漂40%酒精中,让其自然展开,再将分开的切片转移到45℃的温水中展片30秒,用经2%APES丙酮液处理过的载玻片裱贴切片,将制成的组织芯片放入60℃烤箱内烤片2小时,取出室温冷却,放入-4℃冰箱保存。
二、IHC染色及分析
常规二甲苯脱蜡3次,每次6分钟,100%、100%、95%、95%、85%梯度乙醇中水化,每次3分钟,最后自来水冲洗。进行抗原修复,然后将切片放入湿盒中,PBS冲洗3×3分钟。滴加3%H2O2孵育10分钟,PBS冲洗3×3分钟。甩去PBS,滴加过氧化物酶阻断剂室温孵育10分钟。甩干切片,滴加适当比例稀释的一抗(首次稀释根据抗体浓度来设计抗体的稀释比例)室温(25℃)孵育1小时,PBS冲洗3×3分钟,滴加二抗室温孵育30分钟,PBS冲洗3×3分钟,甩去PBS,用新鲜配置的DAB显色液显色3-10分钟。苏木素复染20秒,PBS返蓝。按照85%(3分钟)、95%(3分钟)、95%(3分钟)、100%(3分钟)、100%(3分钟)的酒精梯度依次脱水,最后两次二甲苯透明10分钟,中性树胶封片。
免疫组化染色结果分为:阳性和阴性。阳性表达必须在细胞和组织特定的抗原部位才能视为阳性。在组织染色分布清晰及细胞定位准确的情况下,染色结果根据染色强度的差异进行进一步划分,具体如下:
1、样本为弱阳性;标记为“+”;
2、样本为中度阳性;标记为“++”;
3、样本为高度阳性;标记为“+++”;
4、样本为阴性,标记为“-”。
三、样本检测结果:
用抗p40蛋白单克隆抗体(14D5E8F12)和对照抗体-市售抗p40抗体((ZR8)兔单抗)在18例肺鳞癌和20例肺腺癌上进行同步检测,结果如下表所示。
结果显示:本发明所提供的抗p40蛋白单克隆抗体(14D5E8F12)染色定位准确,染色清晰且无非特异性染色,背景干净。在18例肺鳞癌中,使用抗p40蛋白单克隆抗体(14D5E8F12)的阳性细胞数和阳性强度均高于使用对照试剂,1例对比抗体结果为阴性的样本,抗p40蛋白单克隆抗体(14D5E8F12)结果为弱阳性,2例对比抗体结果为中度阳性的样本,抗p40蛋白单克隆抗体(14D5E8F12)结果为强阳性;说明抗p40蛋白单克隆抗体(14D5E8F12)对肺鳞癌的鉴别诊断相对市售抗体更具敏感度,可有效提高肺鳞癌的检出率。
而在对20例肺腺癌的检测中,本发明所提供的抗p40蛋白单克隆抗体(14D5E8F12)未检出阳性,也证明本抗体对应的可识别抗原在肺鳞癌中特异性表达(检出率和该抗原含量有关),而在肺腺癌中不表达。本抗体可用于肺鳞癌和肺腺癌的鉴别诊断,使用本抗体检出阳性的组织,可确认为肺鳞癌。
而用抗p40蛋白单克隆抗体(14D5E8F12)和对照抗体-市售抗体,在正常组织芯片上进行同步检测,样本阳性和阴性结果一致,说明本抗体在正常组织的特异性同市售抗体相当。
图2为肺鳞癌免疫组化染色结果图,其中左为(14D5E8F12)的p40,右为市售p40((ZR8)兔单抗)。结果显示,14D5E8F12的p40的染色的阳性细胞数和阳性强度明显高于市售p40((ZR8)兔单抗)的染色强度,说明其敏感度更高。
图3为肺腺癌免疫组化染色结果图,其中左为(14D5E8F12)的p40,右为市售p40((ZR8)兔单抗)。结果显示,抗p40蛋白单克隆抗体(14D5E8F12)和对照抗体-市售抗体的免疫组化结果一致,皆为阴性。
序列表
<110> 福州迈新生物技术开发有限公司
<120> 抗p40蛋白单克隆抗体、细胞系及其制备方法和应用
<130> 2020
<160> 5
<170> SIPOSequenceListing 1.0
<210> 1
<211> 16
<212> PRT
<213> 智人(Homo sapiens)
<400> 1
Leu Glu Asn Asn Ala Gln Thr Gln Phe Ser Glu Pro Gln Tyr Thr Asn
1 5 10 15
<210> 2
<211> 133
<212> PRT
<213> 人工序列(Artificial)
<400> 2
Met Glu Trp Leu Trp Asn Leu Leu Phe Leu Met Ala Val Ala Gln Ser
1 5 10 15
Ile Gln Ala Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Val Lys Lys
20 25 30
Pro Gly Glu Thr Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe
35 40 45
Thr Thr Ala Gly Ile Gln Trp Val Gln Met Met Pro Gly Lys Gly Leu
50 55 60
Lys Trp Ile Gly Trp Ile Asn Thr Gln Ser Gly Val Pro Lys Tyr Ala
65 70 75 80
Glu Glu Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser
85 90 95
Thr Ala Tyr Leu Gln Ile Ser Asn Leu Lys Asn Glu Asp Thr Ala Thr
100 105 110
Tyr Phe Cys Ala Ser Gly Pro Leu Ser Tyr Trp Gly Gln Gly Thr Leu
115 120 125
Val Thr Val Ser Ala
130
<210> 3
<211> 131
<212> PRT
<213> 人工序列(Artificial)
<400> 3
Met Ser Pro Ala Gln Phe Leu Phe Leu Leu Val Leu Trp Ile Arg Glu
1 5 10 15
Thr Asn Gly Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val
20 25 30
Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu
35 40 45
Leu Tyr Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro
50 55 60
Gly Gln Ser Pro Met Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser
65 70 75 80
Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr
85 90 95
Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Ile Tyr Tyr Cys
100 105 110
Cys Gln Gly Thr His Phe Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu
115 120 125
Glu Ile Lys
130
<210> 4
<211> 399
<212> DNA
<213> 人工序列(Artificial)
<400> 4
atggaatggc tgtggaactt gctatttctc atggcagtag ctcaaagtat ccaagcacag 60
atccagttgg tgcagtctgg acctgaagtg aagaagcctg gagagacagt caggatctcc 120
tgcaaggcct ctgggtatac cttcacaact gctggaattc agtgggtgca gatgatgcca 180
ggaaagggct tgaagtggat tggctggata aacacccaat ctggagtgcc aaaatatgca 240
gaagaattca agggacggtt tgccttctct ttggaaacct ctgccagcac tgcatattta 300
cagataagca acctcaaaaa tgaggacacg gctacgtatt tctgtgcgag cgggccgctc 360
tcttactggg gccaagggac tctggtcact gtctctgca 399
<210> 5
<211> 393
<212> DNA
<213> 人工序列(Artificial)
<400> 5
atgagtcctg cccagttcct gtttctgtta gtgctctgga ttcgggaaac caacggtgat 60
gttgtgatga cccagactcc actcactttg tcggttacca ttggacaacc ggcctccatc 120
tcttgcaagt caagtcagag cctcttatat agtgatggaa agacatattt gaattggttg 180
ttacagaggc caggccagtc tccaatgcgc ctaatctatc tggtgtctaa actggactct 240
ggagtccctg acaggttcac tggcagtgga tcagggacag atttcacact gaaaatcagc 300
agagtggagg ctgaggattt gggaatttat tattgctgcc aaggaacaca ttttccgtgg 360
acgttcggtg gaggcaccaa gctggaaatc aaa 393
Claims (10)
1.一种抗p40蛋白单克隆抗体,其特征在于,所述单克隆抗体重链和轻链可变区的氨基酸序列分别是SEQ ID NO.2和SEQ ID NO.3所示的氨基酸序列。
2.根据权利要求1所述的单克隆抗体,其特征在于,所述单克隆抗体重链和轻链可变区氨基酸序列分别是SEQ ID NO.4和SEQ ID NO.5所示的核苷酸序列所编码。
3.根据权利要求1所述的单克隆抗体,其特征在于,所述单克隆抗体特异性识别p40蛋白。
4.根据权利要求3所述的单克隆抗体,其特征在于,所述单克隆抗体特异性识别p40蛋白中SEQ ID NO.1所示的氨基酸序列。
5.根据权利要求1所述的单克隆抗体,其特征在于,所述单克隆抗体由保藏号为CGMCCNO.16208的杂交瘤细胞系产生。
6.根据权利要求1所述的单克隆抗体,其特征在于,所述抗p40蛋白单克隆抗体为小鼠IgG1亚型单克隆抗体。
7.根据权利要求1所述的单克隆抗体,其特征在于,所述单克隆抗体的制备过程,选取p40蛋白氨基酸序列第4位至第19位氨基酸并与载体蛋白KLH偶联作为免疫原。
8.一株分泌抗p40蛋白单克隆抗体的杂交瘤细胞系,所述细胞系为小鼠杂交瘤细胞系14D5E8F12,所述细胞系保藏于中国微生物菌种保藏管理委员会普通微生物中心,保藏号为:CGMCC NO.16208。
9.权利要求1-8任一所述的抗p40蛋白单克隆抗体,在p40蛋白免疫检测中的用途。
10.根据权利要求9所述的免疫检测,其特征在于,所述免疫检测包括免疫组织化学法,免疫印迹法和酶联免疫法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011392419.8A CN112409481B (zh) | 2020-12-01 | 2020-12-01 | 抗p40蛋白单克隆抗体、细胞系及其制备方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011392419.8A CN112409481B (zh) | 2020-12-01 | 2020-12-01 | 抗p40蛋白单克隆抗体、细胞系及其制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN112409481A true CN112409481A (zh) | 2021-02-26 |
CN112409481B CN112409481B (zh) | 2024-03-26 |
Family
ID=74829707
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202011392419.8A Active CN112409481B (zh) | 2020-12-01 | 2020-12-01 | 抗p40蛋白单克隆抗体、细胞系及其制备方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112409481B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113845595A (zh) * | 2021-11-16 | 2021-12-28 | 福州迈新生物技术开发有限公司 | 抗gh蛋白单克隆抗体、细胞系及其制备方法和应用 |
CN113845593A (zh) * | 2021-10-27 | 2021-12-28 | 福州迈新生物技术开发有限公司 | 抗α-SMA蛋白单克隆抗体、细胞系及其应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1264425A (zh) * | 1997-07-17 | 2000-08-23 | 皮埃尔法博赫药品公司 | 用于诊断和治疗的呼吸道合胞病毒表位和含有它们的抗体 |
CN111718414A (zh) * | 2020-06-12 | 2020-09-29 | 江苏荃信生物医药有限公司 | 抗人白介素23单克隆抗体及其应用 |
-
2020
- 2020-12-01 CN CN202011392419.8A patent/CN112409481B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1264425A (zh) * | 1997-07-17 | 2000-08-23 | 皮埃尔法博赫药品公司 | 用于诊断和治疗的呼吸道合胞病毒表位和含有它们的抗体 |
CN111718414A (zh) * | 2020-06-12 | 2020-09-29 | 江苏荃信生物医药有限公司 | 抗人白介素23单克隆抗体及其应用 |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113845593A (zh) * | 2021-10-27 | 2021-12-28 | 福州迈新生物技术开发有限公司 | 抗α-SMA蛋白单克隆抗体、细胞系及其应用 |
CN113845593B (zh) * | 2021-10-27 | 2023-03-10 | 福州迈新生物技术开发有限公司 | 抗α-SMA蛋白单克隆抗体、细胞系及其应用 |
CN113845595A (zh) * | 2021-11-16 | 2021-12-28 | 福州迈新生物技术开发有限公司 | 抗gh蛋白单克隆抗体、细胞系及其制备方法和应用 |
CN113845595B (zh) * | 2021-11-16 | 2023-03-10 | 福州迈新生物技术开发有限公司 | 抗gh蛋白单克隆抗体、细胞系及其制备方法和应用 |
Also Published As
Publication number | Publication date |
---|---|
CN112409481B (zh) | 2024-03-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112480260B (zh) | 抗psma蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN112457400B (zh) | 抗β-catenin蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN109734805B (zh) | 抗ck20蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN110218251B (zh) | 抗msh2蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN112442124B (zh) | 抗cd23蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN112194724B (zh) | 抗mpo蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN110903389B (zh) | 抗gfap蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN112940118A (zh) | 抗ck8蛋白的单克隆抗体及其细胞株、制备方法和应用 | |
CN113061186B (zh) | 抗ca125蛋白的单克隆抗体及其细胞株、制备方法和应用 | |
CN111363043A (zh) | 抗cd20蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN112409481B (zh) | 抗p40蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN109485724B (zh) | 抗Desmin蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN111454365B (zh) | 抗msh6蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN109293775B (zh) | 抗pd-1蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN113956356B (zh) | 抗prl蛋白单克隆抗体、细胞系及其应用 | |
CN113735971B (zh) | 抗ck18蛋白单克隆抗体及其细胞株、制备方法和应用 | |
CN114075281B (zh) | 抗Inhibin-α蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN113845592B (zh) | 抗ck5/6蛋白单克隆抗体及其细胞株、制备方法和应用 | |
CN113831410B (zh) | 抗cd56蛋白单克隆抗体及其细胞株、制备方法和应用 | |
CN113845593B (zh) | 抗α-SMA蛋白单克隆抗体、细胞系及其应用 | |
CN113929783B (zh) | 抗cd99蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN114014933B (zh) | 抗plap蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN110922485B (zh) | 抗Ep-cam蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN113943369B (zh) | 抗mum1蛋白单克隆抗体、细胞系及其应用 | |
CN113265000B (zh) | 抗cdx2蛋白的单克隆抗体及其细胞株、制备方法和应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |