CN112402472A - Preparation method of panax notoginseng saponins - Google Patents
Preparation method of panax notoginseng saponins Download PDFInfo
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- CN112402472A CN112402472A CN201910782919.3A CN201910782919A CN112402472A CN 112402472 A CN112402472 A CN 112402472A CN 201910782919 A CN201910782919 A CN 201910782919A CN 112402472 A CN112402472 A CN 112402472A
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- panax notoginseng
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
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- Medical Informatics (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention relates to a preparation method of panax notoginseng saponins, in particular to a method for extracting panax notoginseng saponins from panax notoginseng by adopting a multi-effect wall-breaking low-temperature pipeline method. The processing steps comprise: pulverizing Notoginseng radix, introducing raw materials and water into ultrahigh pressure extractor, extracting under 200MPa, extracting with impinging stream, subjecting the extractive solution to cavitation in cavitation wall-breaking extractor, and then passing through supersonic jet extractor; the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid; and finally drying the obtained concentrated solution by a vacuum belt type dryer to obtain the panax notoginseng saponins. The invention has the characteristics of low labor intensity and excellent operation environment. And the method is operated at normal temperature, the thermosensitive components are not destroyed and decomposed, the target product is effectively dissolved out, the purity is high, the method is environment-friendly, and the comprehensive utilization rate of the circular agriculture is high.
Description
Technical Field
The invention relates to a preparation method of panax notoginseng saponins, belonging to the field of extraction of effective components of traditional Chinese medicines.
Background
Pseudo-ginseng is a peculiar rare Chinese herbal medicine, contains a large amount of active ingredients beneficial to human health, has the effects of treating diseases, protecting health and preserving health, and has wide application prospects in the fields of clinical application, health protection and preserving health and the like. However, the research on the panax notoginseng health-care food in the market is not comprehensive enough, the types of the product formulations are not many, and the related pharmacological action of the panax notoginseng health-care food needs to be further researched, so that the medicines and the health-care food corresponding to the related formulations are researched, and the medicinal value of the panax notoginseng is fully developed.
Panax Notoginsenosides (PNS) belong to dammarane type triterpenoid saponins, and are the main active ingredients of traditional precious Chinese medicinal material Panax notoginsenosides. Panax notoginsenosides have good protective effects on central nervous system, cardiovascular system, cerebrovascular system and immune system, and can be widely used for treating diseases such as aging and tumor.
The Panax Notoginseng Saponins (PNS) are total saponins extracted from the reed head of panax notoginseng of Araliaceae by a certain process, and the study of experts and scholars at home and abroad in recent years shows that the panax notoginseng saponins have the pharmacological effects of prolonging the blood coagulation time, inhibiting the platelet aggregation, reducing the blood fat and the blood pressure, resisting inflammation, resisting oxidation, prolonging the life and the like. At present, the medicine is mainly applied to cardiovascular and cerebrovascular system diseases and central nervous system diseases in clinic.
During the extraction and preparation process of the panax notoginseng saponins, reflux extraction and macroporous resin adsorption are generally adopted, but the methods have the defects of high energy consumption, high loss of active ingredients and the like in the aspects of retaining the active ingredients, removing the ineffective ingredients and the like.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a method for preparing panax notoginseng saponins, which has high efficiency and selectivity, is simple and convenient to operate, has high yield and few byproducts and can be industrially realized.
The technical scheme of the invention is carried out according to the following steps:
a. pulverizing Notoginseng radix, introducing raw materials and water into ultrahigh pressure extractor, extracting under 200MPa, extracting with impinging stream, performing cavitation in cavitation cell wall breaking extractor, and allowing 100% cell wall to disintegrate and then passing through supersonic jet extractor; (ii) a
b. The extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid;
c. drying the obtained concentrated solution with vacuum belt type dryer to obtain Notoginseng radix total saponin.
The pressure of the ultrahigh pressure extractor in the step a is 200MPa, and the power of the motor is 75 KW. After cavitation combination, 100% of the cell walls are dismissed, with fragments of about 20 microns. The secondary metabolite is directly contacted with the solvent, and osmotic pressure is not required to act on the transportation process. The speed of the solute-solvent countercurrent extraction is extremely high, which is about 10 times of the speed of sound.
And (c) in the membrane combination-multistage membrane separation system in the step b, the turbidity of the ceramic membrane permeate is less than or equal to 1, and the concentration process of the membrane concentration equipment is driven by pure pressure.
And c, drying and dehydrating the ultrahigh vacuum by using the vacuum belt dryer, isolating oxygen, and having high drying speed, large evaporation capacity and good product quality.
The invention has the advantages that:
1. the invention adopts a multi-effect wall-breaking low-temperature pipeline extraction method to process the raw materials, realizes the one-step completion of crushing and extraction, and can effectively avoid the damage of the panax notoginseng saponins because the whole production process is not heated and is in low-temperature pipeline production.
2. The method adopts a multi-effect wall-breaking low-temperature pipeline to extract the panax notoginseng saponins, and has the advantages of high speed, high efficiency, high extraction rate and complete retention of the effective components of panax notoginseng.
The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.
The specific implementation mode is as follows:
example 1:
pulverizing Notoginseng radix, introducing raw materials and water into an ultrahigh pressure extractor, extracting under 200MPa, extracting with impinging stream, performing cavitation in a cavitation cell-wall-breaking extractor, placing the material in an ultrasonic device with frequency of 40KHz, and adding 3 times of 50% ethanol solution of the raw materials for ultrasonic extraction for 12 min; the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid; and finally drying the obtained concentrated solution by a vacuum belt type dryer to obtain the panax notoginseng saponins.
Example 2:
pulverizing Notoginseng radix, introducing raw materials and water into an ultrahigh pressure extractor, extracting under 200MPa, extracting with impinging stream, performing cavitation in a cavitation cell-wall-breaking extractor, placing the material in an ultrasonic device with frequency of 40KHz, and adding 60% ethanol solution 5 times of the raw materials for ultrasonic extraction for 12 min; the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid; and finally drying the obtained concentrated solution by a vacuum belt type dryer to obtain the panax notoginseng saponins.
Example 3:
pulverizing Notoginseng radix, introducing raw materials and water into an ultrahigh pressure extractor, extracting under 200MPa, extracting with impinging stream, performing cavitation in a cavitation cell-wall-breaking extractor, placing the material in an ultrasonic device with frequency of 40KHz, and adding 55% ethanol solution 4 times of the raw materials for ultrasonic extraction for 12 min; the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid; and finally drying the obtained concentrated solution by a vacuum belt type dryer to obtain the panax notoginseng saponins.
Claims (4)
1. A method for preparing panax notoginseng saponins is characterized in that:
a. pulverizing Notoginseng radix, introducing raw materials and water into ultrahigh pressure extractor, extracting under 200MPa, extracting with impinging stream, performing cavitation in cavitation cell wall breaking extractor, and allowing 100% cell wall to disintegrate and then passing through supersonic jet extractor;
b. the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid;
c. drying the obtained concentrated solution with vacuum belt type dryer to obtain Notoginseng radix total saponin.
2. The method for preparing panax notoginseng saponins according to claim 1, wherein the pressure of the ultra-high pressure extractor in the step a is 200MPa, and the motor power is 75 KW. After cavitation combination, 100% of the cell walls are dismissed, with fragments of about 20 microns. The secondary metabolite is directly contacted with the solvent, and osmotic pressure is not required to act on the transportation process. The speed of the solute-solvent countercurrent extraction is extremely high, which is about 10 times of the speed of sound.
3. The method for preparing panax notoginseng saponins according to claim 1, wherein the turbidity of the ceramic membrane permeate in the membrane combination-multistage membrane separation system in the step b is less than or equal to 1, and the membrane concentration equipment is driven by pure pressure in the concentration process.
4. The method for preparing panax notoginseng saponins according to claim 1, wherein the vacuum belt dryer dries and dehydrates in ultra-high vacuum in the step c, and has the advantages of oxygen isolation, fast drying speed, large evaporation capacity and good product quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910782919.3A CN112402472A (en) | 2019-08-23 | 2019-08-23 | Preparation method of panax notoginseng saponins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910782919.3A CN112402472A (en) | 2019-08-23 | 2019-08-23 | Preparation method of panax notoginseng saponins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112402472A true CN112402472A (en) | 2021-02-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910782919.3A Withdrawn CN112402472A (en) | 2019-08-23 | 2019-08-23 | Preparation method of panax notoginseng saponins |
Country Status (1)
| Country | Link |
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| CN (1) | CN112402472A (en) |
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2019
- 2019-08-23 CN CN201910782919.3A patent/CN112402472A/en not_active Withdrawn
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Application publication date: 20210226 |
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| WW01 | Invention patent application withdrawn after publication |