CN111202768A - Preparation method of tea flower extract - Google Patents
Preparation method of tea flower extract Download PDFInfo
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- CN111202768A CN111202768A CN201811394855.1A CN201811394855A CN111202768A CN 111202768 A CN111202768 A CN 111202768A CN 201811394855 A CN201811394855 A CN 201811394855A CN 111202768 A CN111202768 A CN 111202768A
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- membrane
- flower extract
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
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- Medical Informatics (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Extraction Or Liquid Replacement (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a preparation method of a tea flower extract, and particularly relates to a method for extracting a tea flower extract from tea flowers by adopting a multi-effect wall-breaking integrated low-temperature extraction method. The processing steps comprise: drying and crushing tea flowers, extracting the raw materials at the pressure of 200MPa by an ultrahigh pressure extractor, then performing impinging stream extraction, then performing cavitation action in a cavitation wall-breaking extractor, and then passing through a supersonic jet extractor; the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid; and finally drying the obtained concentrated solution by a vacuum belt type dryer to obtain the tea flower extract. The invention operates at normal temperature, does not destroy and decompose heat-sensitive components, effectively dissolves out target products, and has the advantages of environmental protection, high comprehensive utilization rate of cyclic agriculture and the like.
Description
Technical Field
The invention relates to a preparation method of a tea flower extract, belonging to the field of extraction of effective components of traditional Chinese medicines.
Background
The tea flower contains components with the effects of detoxifying, inhibiting bacteria, reducing blood sugar, delaying aging, preventing and resisting cancer, enhancing immunity and the like, the content of protein, tea polysaccharide, tea polyphenol and active antioxidant substances exceeds the content of similar substances in tea, and the content of pesticide residue and heavy metal is lower than the European Union standard. The tea flower is a high-quality protein nutrient source and can be compared favorably with the antioxidant plant rosemary recognized in the world.
The tea flower extract is a pure natural compound extracted from tea flowers, is a novel antioxidant, and has good effects of detoxifying, reducing blood lipid, reducing blood sugar, resisting aging, resisting cancer, nourishing, strengthening body, beautifying and the like.
During the extraction and preparation process of the tea flower extract, reflux extraction and macroporous resin adsorption are generally adopted, but the methods have the defects of high energy consumption, high loss of active ingredients and the like in the aspects of retaining the active ingredients, removing the ineffective ingredients and the like.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and aims to provide a method for preparing the camellia sinensis flower extract, which has high efficiency and selectivity, is simple and convenient to operate, has high yield and few byproducts and can be industrially realized.
The technical scheme of the invention is carried out according to the following steps:
a. drying tea flower in the shade, pulverizing, extracting raw materials with 200MPa pressure in an ultrahigh pressure extractor, extracting with impinging stream, performing cavitation in a cavitation wall-breaking extractor, and allowing 100% cell wall to be decomposed and pass through a supersonic jet extractor; (ii) a
b. The extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid;
c. drying the obtained concentrated solution with vacuum belt dryer to obtain tea flower extract.
The pressure of the ultrahigh pressure extractor in the step a is 200MPa, and the power of the motor is 75 KW. After cavitation combination, 100% of the cell walls are dismissed, with fragments of about 20 microns. The secondary metabolite is directly contacted with the solvent, and osmotic pressure is not required to act on the transportation process. The speed of the solute-solvent countercurrent extraction is extremely high, which is about 10 times of the speed of sound.
And (c) in the membrane combination-multistage membrane separation system in the step b, the turbidity of the ceramic membrane permeate is less than or equal to 1, and the concentration process of the membrane concentration equipment is driven by pure pressure.
And c, drying and dehydrating the ultrahigh vacuum by using the vacuum belt dryer, isolating oxygen, and having high drying speed, large evaporation capacity and good product quality.
The invention has the advantages that:
1. the invention adopts a multi-effect wall-breaking low-temperature pipeline extraction method to process the raw materials, realizes the one-step completion of crushing and extraction, and simultaneously can effectively avoid the damage of the tea flower extract because the whole production process is not heated and is in low-temperature pipeline production.
2. The method adopts a multi-effect wall-breaking low-temperature pipeline to extract the tea flower extract, and has the advantages of high speed, high efficiency, high extraction rate and complete retention of the effective components of the tea flower.
The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.
The specific implementation mode is as follows:
example 1:
drying and crushing tea flowers, extracting the raw materials at the pressure of 200MPa through an ultrahigh pressure extractor, then performing impinging stream extraction, then performing cavitation action in a cavitation wall breaking extractor, then placing the materials in an ultrasonic device with the frequency of 40KHz, and adding an ethanol solution which is 3 times that of the raw materials and is 50% of the raw materials to perform ultrasonic extraction for 10 min; the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid; and finally drying the obtained concentrated solution by a vacuum belt type dryer to obtain the tea flower extract. The content of the tea flower extract is 97.46% by HPLC detection.
Example 2:
drying and crushing tea flowers, extracting the raw materials by an ultrahigh pressure extractor under the pressure of 200MPa, then performing impinging stream extraction, then performing cavitation in a cavitation wall-breaking extractor, then placing the materials in an ultrasonic device with the frequency of 40KHz, and adding 60% ethanol solution which is 5 times of the raw materials to perform ultrasonic extraction for 10 min; the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid; and finally drying the obtained concentrated solution by a vacuum belt type dryer to obtain the tea flower extract. The content of the tea flower extract is 97.87% by HPLC detection.
Example 3:
drying and crushing tea flowers, extracting the raw materials by an ultrahigh pressure extractor under the pressure of 200MPa, then performing impinging stream extraction, then performing cavitation in a cavitation wall-breaking extractor, then placing the materials in an ultrasonic device with the frequency of 40KHz, and adding 55% ethanol solution which is 4 times of the raw materials to perform ultrasonic extraction for 10 min; the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid; and finally drying the obtained concentrated solution by a vacuum belt type dryer to obtain the tea flower extract. The content of the tea flower extract is 97.56% by HPLC detection.
Claims (4)
1. A preparation method of a tea flower extract is characterized by comprising the following steps:
a. drying tea flower, pulverizing, extracting raw materials with 200MPa pressure in ultrahigh pressure extractor, extracting with impinging stream, performing cavitation in cavitation cell wall breaking extractor, and allowing 100% cell wall to be decomposed by supersonic jet extractor;
b. the extracting solution enters a membrane combined-multistage membrane separation system after passing through a filter-pressing filter-aid separation system, and then enters a resin column separation system to obtain a separation solution; concentrating the separated liquid by a membrane combination-multistage membrane concentration system to obtain a concentrated liquid;
c. drying the obtained concentrated solution with vacuum belt dryer to obtain tea flower extract.
2. The method for preparing the camellia sinensis flower extract according to claim 1, wherein the pressure of the ultrahigh pressure extractor in the step a is 200MPa, and the motor power is 75 KW. After cavitation combination, 100% of the cell walls are dismissed, with fragments of about 20 microns. The secondary metabolite is directly contacted with the solvent, and osmotic pressure is not required to act on the transportation process. The speed of the solute-solvent countercurrent extraction is extremely high, which is about 10 times of the speed of sound.
3. The method for preparing the camellia sinensis flower extract according to claim 1, wherein the turbidity of the ceramic membrane permeate in the membrane combination-multistage membrane separation system in the step b is less than or equal to 1, and the concentration process of the membrane concentration equipment is driven by pure pressure.
4. The method for preparing the camellia sinensis flower extract as claimed in claim 1, wherein the vacuum belt dryer dries and dehydrates in ultra-high vacuum in step c, and is oxygen-free, fast in drying speed, large in evaporation amount and good in product quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811394855.1A CN111202768A (en) | 2018-11-22 | 2018-11-22 | Preparation method of tea flower extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811394855.1A CN111202768A (en) | 2018-11-22 | 2018-11-22 | Preparation method of tea flower extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111202768A true CN111202768A (en) | 2020-05-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811394855.1A Pending CN111202768A (en) | 2018-11-22 | 2018-11-22 | Preparation method of tea flower extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111202768A (en) |
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2018
- 2018-11-22 CN CN201811394855.1A patent/CN111202768A/en active Pending
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Application publication date: 20200529 |