CN112401241A - Cod-liver oil nano-emulsion and preparation method thereof - Google Patents
Cod-liver oil nano-emulsion and preparation method thereof Download PDFInfo
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- 235000012716 cod liver oil Nutrition 0.000 title claims abstract description 77
- 239000007908 nanoemulsion Substances 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
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- 241000196252 Ulva Species 0.000 claims abstract description 31
- 239000000243 solution Substances 0.000 claims abstract description 30
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- 229920001282 polysaccharide Polymers 0.000 claims abstract description 28
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- 241000251468 Actinopterygii Species 0.000 claims abstract description 22
- 239000000839 emulsion Substances 0.000 claims abstract description 17
- 239000008055 phosphate buffer solution Substances 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 9
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- SBJKKFFYIZUCET-JLAZNSOCSA-N Dehydro-L-ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(=O)C1=O SBJKKFFYIZUCET-JLAZNSOCSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 description 1
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Images
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/106—Production of fats or fatty oils from raw materials by extracting using ultra-sounds
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/06—Refining fats or fatty oils by chemical reaction with bases
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
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- C11B3/00—Refining fats or fatty oils
- C11B3/12—Refining fats or fatty oils by distillation
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention relates to a cod-liver oil nano-emulsion and a preparation method thereof, wherein the preparation method comprises the following steps: dissolving Enteromorpha polysaccharide in a phosphate buffer solution to obtain a first solution; dissolving tween 80 in a phosphate buffer solution to obtain a second solution; mixing the first solution and the second solution, and adding a phosphate buffer solution to be used as a water phase; adding cod liver oil into the water phase, and dispersing in a high-speed shearing dispersion machine for 5min to obtain a coarse emulsion; and (3) circularly homogenizing the coarse emulsion for multiple times to obtain the cod-liver oil nano-emulsion. According to the method, the enteromorpha polysaccharide is added into a dispersion system of the fish liver oil nano-emulsion, so that the stability of the fish liver oil nano-emulsion can be improved, the functional components of the fish liver oil nano-emulsion can be increased, the addition of a surfactant can be reduced, and polyphenol substances contained in the enteromorpha crude polysaccharide can inhibit the fishy smell of the fish liver oil to a certain extent; thereby obtaining the cod-liver oil nanoemulsion which has better taste, is beneficial to the health of the human body and is easy to be absorbed by the human body.
Description
Technical Field
The invention relates to the technical field of cod-liver oil, and in particular relates to a cod-liver oil nano-emulsion and a preparation method thereof.
Background
The natural fish liver oil is a fatty oil which is extracted from fish livers and is rich in vitamin A, vitamin D3, DHA, EPA, squalene and various omega-3 polyunsaturated fatty acids, and has the effects of improving immunity and preventing and treating cardiovascular and cerebrovascular diseases. With the development of the cod liver oil industry, more and more researches show that the natural cod liver oil also has the functions of resisting inflammation, softening tissue vessels, improving emotion and cognitive functions, reducing the risk of type II diabetes, protecting bone health, nourishing skin, promoting natural weight loss and the like.
In recent years, with the increasing share of cod liver oil products in the markets of medicines and health care products year by year, two major problems, namely oxidation and fishy smell, are often encountered in the production and application processes of cod liver oil, which becomes a problem to be solved urgently in the application of cod liver oil. The factors causing the oxidation of the cod liver oil mainly proceed in an autoxidation mode, follow the mechanism of a free radical chain reaction, and mainly comprise internal factors and environmental factors: (1) the key factor in this process is that the molecular chain of polyunsaturated fatty acid contains double bonds to cause oxidation. The more the number of double bonds, the higher the degree of unsaturation, the easier it is to oxidize; (2) environmental factors causing the autooxidation of polyunsaturated fatty acids are mainly oxygen, temperature, light and metal ions. Oxygen is the most important factor in causing autoxidation of polyunsaturated fatty acids, and the greater the degree of contact with oxygen, the faster the rate of oxidation.
In order to control the oxidation of the cod liver oil, improve the stability of the cod liver oil and conveniently apply the cod liver oil to medicines and health care products, the current measures are mainly to use antioxidants and microencapsulation. Although the microcapsule product sold in the market can delay the oxidation speed to a certain extent, the microcapsule has large grain diameter which is generally in the micron order, and the utilization rate of human body is low; the application is inconvenient, and the dispersibility is poor when the product is added into food; the production process is complex and the cost is high. Although the emulsion is a thermodynamically unstable system, the emulsion is convenient to use in food and medicine because of its small particle size and narrow distribution range. Modern research has shown that, if the cod liver oil is made into particles smaller than 500 nm, the cod liver oil can be directly absorbed by intestinal canal walls without the action of digestive enzymes in the intestinal canal; if the nano scientific technology is adopted, the cod liver oil is prepared into particles smaller than 100 nanometers, and the cod liver oil nanoparticles can be directly absorbed by oral cavity, nasal cavity and other mucous membranes, so that the absorption of the cod liver oil by a human body is greatly improved, and the effect is more remarkable especially for middle-aged and old people with declined metabolic function and infants with incomplete metabolic function development.
However, the fish liver oil emulsion in the related technology has the problems of high content of surfactant and cosurfactant, complex operation, difficult control of stirring speed and the like, is unfavorable for mouth feel and human health when being used as a medicine or a health-care product, and is not beneficial to being applied to industrial production.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the technical problems in the related art. Therefore, the invention aims to provide the cod liver oil nanoemulsion and the preparation method thereof.
To this end, according to an embodiment of the present invention, there is provided a method for preparing a cod liver oil nanoemulsion, which includes:
dissolving Enteromorpha polysaccharide in a phosphate buffer solution to obtain a first solution;
dissolving tween 80 in a phosphate buffer solution to obtain a second solution;
mixing the first solution and the second solution, and adding a phosphate buffer solution to be used as a water phase;
adding cod liver oil into the water phase, and dispersing in a high-speed shearing dispersion machine for 5min to obtain a coarse emulsion;
and (3) circularly homogenizing the coarse emulsion for multiple times to obtain the cod-liver oil nano-emulsion.
According to the preparation method of the fish liver oil nanoemulsion, provided by the embodiment of the invention, the enteromorpha polysaccharide is added into a dispersion system of the fish liver oil nanoemulsion, so that the stability of the fish liver oil nanoemulsion can be improved, the functional components of the fish liver oil nanoemulsion can be increased, the addition of a surfactant can be reduced, and polyphenol substances contained in the enteromorpha crude polysaccharide can inhibit the fishy smell of the fish liver oil to a certain extent; thereby obtaining the cod-liver oil nanoemulsion which has better taste, is beneficial to the health of the human body and is easy to be absorbed by the human body.
In addition, the preparation method of the cod-liver oil nanoemulsion provided by the above embodiment of the invention can also have the following additional technical characteristics:
optionally, the addition amount of the Enteromorpha polysaccharide is 0.5-3.0 g.
Optionally, the weight ratio of the cod liver oil to the aqueous phase is 1: 19.
Alternatively, tween 80 is added in an amount of 1.0 g.
Optionally, the operating conditions of the high shear disperser are 2000 rpm.
Optionally, the pressure of the homogenization treatment is 120 MPa.
Optionally, the preparation of the cod liver oil comprises:
cutting frozen sea purse liver into pieces, pouring the cut pieces into a colloid mill after the pieces are slightly softened, opening a reflux passage, and performing reflux crushing for 15min to obtain fish liver pulp;
ultrasonic thawing the fish liver pulp for 2.5h, centrifuging after thawing, and collecting upper crude oil;
adding 40% citric acid and phosphoric acid into the upper layer crude oil, heating at 50 deg.C for 30min, adding sodium hydroxide, heating at 85 deg.C for 20min, and centrifuging to obtain deacidified cod liver oil;
washing the deacidified cod liver oil with hot saline water at 95 ℃ for multiple times until the pH value is 7, adding activated clay into the washed deacidified cod liver oil, heating at 70 ℃ for 20min, and centrifuging to obtain decolored cod liver oil;
and distilling the decolored cod liver oil under reduced pressure for 60min to obtain refined cod liver oil.
Optionally, the preparation of the enteromorpha polysaccharide comprises:
adding water into Enteromorpha powder, boiling at 100 deg.C for 5 hr, centrifuging, and collecting supernatant;
vacuum concentrating the supernatant for 10 times, adding 95% ethanol with volume 3 times of the supernatant, stirring uniformly, standing overnight, centrifuging, and collecting precipitate;
and washing the precipitate with 95% ethanol, and drying under reduced pressure to obtain the Enteromorpha polysaccharide.
According to the embodiment of the invention, the invention also provides a cod liver oil nanoemulsion prepared by adopting the preparation method.
According to the embodiment of the invention, the cod liver oil nanoemulsion which has better mouthfeel, is beneficial to human health and is easy to absorb by a human body can be obtained by adopting the preparation method.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 is a graph showing the influence of the addition amount of Enteromorpha polysaccharide on the particle size of a cod liver oil nanoemulsion according to an embodiment of the invention;
FIG. 2 is a graph showing the amount of hydroperoxide produced during storage of a cod liver oil nanoemulsion according to an embodiment of the present invention;
Detailed Description
The technical solution of the present invention is illustrated by specific examples below. It is to be understood that one or more method steps mentioned in the present invention do not exclude the presence of other method steps before or after the combination step or that other method steps may be inserted between the explicitly mentioned steps; it should also be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Moreover, unless otherwise indicated, the numbering of the various method steps is merely a convenient tool for identifying the various method steps, and is not intended to limit the order in which the method steps are arranged or the scope of the invention in which the invention may be practiced, and changes or modifications in the relative relationship may be made without substantially changing the technical content.
In order to better understand the above technical solutions, exemplary embodiments of the present invention are described in more detail below. While exemplary embodiments of the invention have been shown, it should be understood that the invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
The test materials adopted by the invention are all common commercial products and can be purchased in the market.
The invention will now be described with reference to specific examples, which are intended to be illustrative only and not to be limiting in any way.
Example 1
Extraction of cod liver oil
(1) Cutting 1.5kg frozen sea purse liver (crude fat content is 420.6g) into small pieces, slightly softening, pouring into colloid mill, opening reflux path, reflux-pulverizing for 15min to obtain fish liver pulp, and packaging with centrifuge bottle, and labeling.
(2) Placing the centrifugal bottle into a water bath of a bath type ultrasonic machine filled with distilled water, and inserting a temperature probe into the geometric center of the liver of the sea purse at 0.5w/cm2The ultrasonic thawing is carried out under the ultrasonic condition, and when the central temperature of the liver of the ray reaches 0 ℃, the thawing time is 2.5 hours; centrifuging the thawed liver of the raja for 10min at the temperature of 25 ℃ and the speed of 10000r/min to obtain 391.2g of upper layer crude oil, namely the liver oil of the raja.
(3) Refining 100g of raja roughetti liver oil, adding 2g of 40% citric acid and 3g of phosphoric acid into the raja roughetti liver oil, magnetically stirring and heating at 50 ℃ for 30min, taking out, adding 20g of sodium hydroxide (mass fraction is 20%), magnetically stirring and heating at 85 ℃ for 20min, centrifuging under the condition of 10000r/min for 10min, and obtaining 81.5g of deacidified raja liver oil. Washing the obtained deacidified cod liver oil with hot saline (5% by mass) at 95 ℃ for five to eight times, wherein the dosage is 20ml each time, washing until the pH is 7, adding 8g of activated clay into the washed deacidified cod liver oil, heating for 20min at 70 ℃ by magnetic stirring, centrifuging under the centrifugal condition of 10000r/min for 10min, and obtaining 73.8g of decolored cod liver oil. Pouring the decolorized cod liver oil into a distillation flask, and distilling under reduced pressure at 40 deg.C for 60min under 0.06MPa for 60min to obtain refined cod liver oil 70.6 g.
Extraction of Enteromorpha polysaccharide
(1) Weighing 500g of enteromorpha powder, adding 20L of pure water, boiling for 5h at 100 ℃, centrifuging and collecting supernatant;
(2) concentrating the collected supernatant in vacuum by 10 times, adding 95% ethanol with the volume of 3 times of that of the collected supernatant, uniformly stirring, standing overnight, centrifuging and collecting precipitates;
(3) washing the precipitate with 95% ethanol, drying under reduced pressure, and collecting 51.3g of Enteromorpha polysaccharide.
Example 2
Preparation of cod liver oil nano-emulsion
(1) 0.5g of the Enteromorpha polysaccharide obtained in example 1 was weighed and dissolved in 50mL of a phosphate buffer solution (pH7.0) to obtain a first solution.
(2) A second solution was obtained by dissolving 1.0g of Tween 80 in 30mL of a phosphate buffer solution (pH 7.0).
(3) The first solution and the second solution were mixed, and then a phosphate buffer (pH7.0) to 95g was added as an aqueous phase.
(4) 5g of the refined cod liver oil obtained in example 1 was added to the aqueous phase, and the mixture was dispersed in a high-speed shear disperser at 20000rpm for 5min to obtain a crude emulsion.
(5) And (3) circulating the coarse emulsion under the pressure of 120MPa for 3 times of homogenization treatment to obtain the cod-liver oil nano emulsion.
Example 3
Preparation of cod liver oil nano-emulsion
(1) 1.0g of the Enteromorpha polysaccharide obtained in example 1 was weighed and dissolved in 50mL of a phosphate buffer solution (pH7.0) to obtain a first solution.
(2) A second solution was obtained by dissolving 1.0g of Tween 80 in 30mL of a phosphate buffer solution (pH 7.0).
(3) The first solution and the second solution were mixed, and then a phosphate buffer (pH7.0) to 95g was added as an aqueous phase.
(4) 5g of the refined cod liver oil obtained in example 1 was added to the aqueous phase, and the mixture was dispersed in a high-speed shear disperser at 20000rpm for 5min to obtain a crude emulsion.
(5) And (3) circulating the coarse emulsion under the pressure of 120MPa for 3 times of homogenization treatment to obtain the cod-liver oil nano emulsion.
Example 4
Preparation of cod liver oil nano-emulsion
(1) 2.0g of the Enteromorpha polysaccharide obtained in example 1 was weighed and dissolved in 50mL of a phosphate buffer solution (pH7.0) to obtain a first solution.
(2) A second solution was obtained by dissolving 1.0g of Tween 80 in 30mL of a phosphate buffer solution (pH 7.0).
(3) The first solution and the second solution were mixed, and then a phosphate buffer (pH7.0) to 95g was added as an aqueous phase.
(4) 5g of the refined cod liver oil obtained in example 1 was added to the aqueous phase, and the mixture was dispersed in a high-speed shear disperser at 20000rpm for 5min to obtain a crude emulsion.
(5) And (3) circulating the coarse emulsion under the pressure of 120MPa for 3 times of homogenization treatment to obtain the cod-liver oil nano emulsion.
Example 5
Preparation of cod liver oil nano-emulsion
(1) 3.0g of the Enteromorpha polysaccharide obtained in example 1 was weighed and dissolved in 50mL of a phosphate buffer solution (pH7.0) to obtain a first solution.
(2) A second solution was obtained by dissolving 1.0g of Tween 80 in 30mL of a phosphate buffer solution (pH 7.0).
(3) The first solution and the second solution were mixed, and then a phosphate buffer (pH7.0) to 95g was added as an aqueous phase.
(4) 5g of the refined cod liver oil obtained in example 1 was added to the aqueous phase, and the mixture was dispersed in a high-speed shear disperser at 20000rpm for 5min to obtain a crude emulsion.
(5) And (3) circulating the coarse emulsion under the pressure of 120MPa for 3 times of homogenization treatment to obtain the cod-liver oil nano emulsion.
Test examples
1. And (3) determining the particle size of the cod-liver oil nanoemulsion: 10 mu L of the cod-liver oil nanoemulsion is diluted by 1000 times with water for three times, the droplet sizes of the cod-liver oil nanoemulsion with different enteromorpha polysaccharide contents are detected by using a Zetasizer NanoZS particle size potentiostat (Malvern Instruments), the detection temperature is 25 ℃, and an average value is obtained by measuring each sample for three times.
The result is shown in fig. 1, and the average particle size of the cod-liver oil nanoemulsion has a certain rising trend along with the increase of the content of the enteromorpha polysaccharide. When the content of the enteromorpha polysaccharide exceeds 1%, the average particle size of the nanoemulsion is remarkably improved.
2. Stability of
As shown in figure 2, all the cod liver oil nanoemulsion samples gradually form hydroperoxide in the storage period of 12 days, wherein the samples without enteromorpha polysaccharide reach 6 mu M on the 12 th day, while the samples containing enteromorpha polysaccharide are about 1 mu M, which shows that the enteromorpha polysaccharide has obvious effect of inhibiting oil and fat oxidation in the cod liver oil nanoemulsion.
In conclusion, according to the preparation method of the fish liver oil nanoemulsion, provided by the embodiment of the invention, the enteromorpha polysaccharide is added into the dispersion system of the fish liver oil nanoemulsion, so that the stability of the fish liver oil nanoemulsion can be improved, the functional components of the fish liver oil nanoemulsion can be increased, the addition of a surfactant can be reduced, and the polyphenol substances contained in the enteromorpha crude polysaccharide can inhibit the fishy smell of the fish liver oil to a certain extent; thereby obtaining the cod-liver oil nanoemulsion which has better taste, is beneficial to the health of the human body and is easy to be absorbed by the human body.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (9)
1. A preparation method of a cod-liver oil nanoemulsion is characterized by comprising the following steps:
dissolving Enteromorpha polysaccharide in a phosphate buffer solution to obtain a first solution;
dissolving tween 80 in a phosphate buffer solution to obtain a second solution;
mixing the first solution and the second solution, and adding a phosphate buffer solution to be used as a water phase;
adding cod liver oil into the water phase, and dispersing in a high-speed shearing dispersion machine for 5min to obtain a coarse emulsion;
and (3) circularly homogenizing the coarse emulsion for multiple times to obtain the cod-liver oil nano-emulsion.
2. The preparation method according to claim 1, wherein the addition amount of the Enteromorpha polysaccharide is 0.5-3.0 g.
3. The method of claim 1, wherein the weight ratio of the cod liver oil to the aqueous phase is 1: 19.
4. The method according to claim 1, wherein tween 80 is added in an amount of 1.0 g.
5. The production method according to claim 1, wherein the operating condition of the high shear disperser is 2000 rpm.
6. The method according to claim 1, wherein the pressure of the homogenization treatment is 120 MPa.
7. The method of claim 1, wherein the preparation of the cod liver oil comprises:
cutting frozen sea purse liver into pieces, pouring the cut pieces into a colloid mill after the pieces are slightly softened, opening a reflux passage, and performing reflux crushing for 15min to obtain fish liver pulp;
ultrasonic thawing the fish liver pulp for 2.5h, centrifuging after thawing, and collecting upper crude oil;
adding 40% citric acid and phosphoric acid into the upper layer crude oil, heating at 50 deg.C for 30min, adding sodium hydroxide, heating at 85 deg.C for 20min, and centrifuging to obtain deacidified cod liver oil;
washing the deacidified cod liver oil with hot saline water at 95 ℃ for multiple times until the pH value is 7, adding activated clay into the washed deacidified cod liver oil, heating at 70 ℃ for 20min, and centrifuging to obtain decolored cod liver oil;
and distilling the decolored cod liver oil under reduced pressure for 60min to obtain refined cod liver oil.
8. The preparation method according to claim 1, wherein the preparation of the Enteromorpha polysaccharide comprises:
adding water into Enteromorpha powder, boiling at 100 deg.C for 5 hr, centrifuging, and collecting supernatant;
vacuum concentrating the supernatant for 10 times, adding 95% ethanol with volume 3 times of the supernatant, stirring uniformly, standing overnight, centrifuging, and collecting precipitate;
and washing the precipitate with 95% ethanol, and drying under reduced pressure to obtain the Enteromorpha polysaccharide.
9. A cod liver oil nanoemulsion characterized by being produced by the production method according to any one of claims 1 to 8.
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| JPH11292773A (en) * | 1998-04-10 | 1999-10-26 | Nof Corp | Cod liver oil, purification and use |
| CN103564067A (en) * | 2012-07-27 | 2014-02-12 | 浙江海洋学院 | Skate liver oil and preparation method thereof |
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| CN111575103A (en) * | 2020-05-09 | 2020-08-25 | 自然资源部第三海洋研究所 | Skate liver oil and preparation method thereof |
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| JPH11292773A (en) * | 1998-04-10 | 1999-10-26 | Nof Corp | Cod liver oil, purification and use |
| CN103564067A (en) * | 2012-07-27 | 2014-02-12 | 浙江海洋学院 | Skate liver oil and preparation method thereof |
| CN104000780A (en) * | 2014-06-11 | 2014-08-27 | 青岛双鲸药业有限公司 | Three-dimensional cod-liver oil emulsion |
| CN108531290A (en) * | 2018-02-05 | 2018-09-14 | 浙江工商大学 | Inhibit the method for lipid oxidation in fish oil |
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