CN112385669A - Bactericidal composition and application thereof - Google Patents
Bactericidal composition and application thereof Download PDFInfo
- Publication number
- CN112385669A CN112385669A CN202011392983.XA CN202011392983A CN112385669A CN 112385669 A CN112385669 A CN 112385669A CN 202011392983 A CN202011392983 A CN 202011392983A CN 112385669 A CN112385669 A CN 112385669A
- Authority
- CN
- China
- Prior art keywords
- fluopyram
- pyrimidine nucleoside
- days
- pesticide
- control effect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bactericidal composition and application thereof, belonging to the field of pesticides, wherein the effective components of the bactericidal composition comprise pyrimidine nucleoside antibiotics and fluopyram, and the weight ratio of the pyrimidine nucleoside antibiotics to the fluopyram is (1-50) to (50-1). Compared with a single agent, the composition has obvious synergistic effect after being compounded, and the control effect is improved; the compounding of the medicament reduces the dosage, reduces the cost and lightens the pollution to the environment; has better control effect on rice sheath blight, tomato gray mold and watermelon fusarium wilt, and has wide application range.
Description
Technical Field
The invention relates to a bactericidal composition, in particular to a bactericidal composition containing pyrimidine nucleoside antibiotics and fluopyram and application thereof.
Background
The rice sheath blight disease is one of main diseases damaging rice, and is also called foot rot disease and rice stem blast. The disease is common in rice production. From the growth cycle of rice, except that diseases do not occur in the growth period of seedlings, the diseases occur in other growth periods, the rice is lodging and withering due to serious diseases, and the yield of the rice is seriously influenced if the diseases are not prevented and controlled in time.
The tomato gray mold is a relatively serious and common disease on tomatoes, occurs in all vegetable areas, and can damage 20 kinds of crops such as eggplants, hot peppers, cucumbers, bottle gourds and the like besides tomatoes. The low-temperature continuous rainy weather is serious in year harm, and the stem and leaf withering, a large amount of burnt-out flowers and fruits are caused in serious disease, so that the yield is directly influenced.
Watermelon wilt is a devastating disease of watermelon and is widely distributed in all watermelon producing areas in the world. With the adjustment of agricultural industrial structure in China, the watermelon planting area is larger and larger, and continuous cropping obstacles become important factors restricting watermelon production. The blight is one of the main diseases of watermelon continuous cropping obstacle. With the continuous cropping of watermelons year by year, the watermelon fusarium wilt is more and more serious, the chemical prevention and control is an important and effective method for controlling the watermelon fusarium wilt at present, but the long-term use of chemical bactericides not only causes the drug resistance of the watermelon fusarium wilt to increase year by year, but also causes the problems of pesticide residue and the like, and with the development of modern society, people pay attention to environmental protection and self health, and need to develop a high-efficiency and low-toxicity biological pesticide urgently.
Pyrimidine nucleoside antibiotics are also called antimycotic and agroinhibin 120. The metabolite of streptomyces spinosus Beijing variant is an alkaline nucleoside antibiotic, and has strong inhibiting and treating effects on a plurality of plant pathogenic bacteria. The sterilization mechanism is to inhibit the protein synthesis of pathogenic bacteria, so that the pathogenic bacteria die. Good adhesion, strong penetrating power and systemic action, can quickly permeate into the cortex, enter into the body of crops and resist the invasion of pathogenic bacteria, and has a lasting period of more than 15 days. Has broad antibacterial spectrum, and has both prevention and protection effects and therapeutic effect. The pyrimidine nucleoside antibiotics are used for preventing and treating powdery mildew, epidemic disease, flower powdery mildew, black spot and the like of vegetables and fruits, and the pyrimidine nucleoside antibiotics are used for preventing and treating rice sheath blight, so that the prevention effect is reduced with long-term use.
Fluopyram (ISO common name: Fluopyram), chemical name: n- {2- [ 3-chloro-5- (trifluoromethyl) -2-pyridinyl ] ethyl } -alpha, alpha-ortho-trifluoromethylbenzamide. Fluopyram is a SDHI (succinate dehydrogenase inhibitor) bactericide and nematicide developed by Bayer crop science, and is used for preventing and controlling alternaria leaf spot, gray mold, powdery mildew, sclerotinia, early blight, sheath blight and the like on vegetables such as grapes, pear trees, bananas, apples, cucumbers, tomatoes, field crops and the like. The disadvantage of fluopyram is that the use cost is high, and there is a pressing need to reduce the use cost of fluopyram.
In addition, the frequent use of the bactericide with a single active component causes the drug resistance of most germs, and the control effect is not ideal, so that a novel product is urgently needed to replace the bactericide with a single active component, the drug resistance risk of diseases is delayed by increasing the drug effect, and the economic loss is reduced.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a bactericidal composition to meet the needs of agricultural production, active components contained in the composition have synergistic effect mutually, can improve the simulation and is beneficial to delaying the generation of drug resistance of germs.
The invention is realized by the following technical scheme:
the bactericidal composition comprises the effective components of pyrimidine nucleoside antibiotics and fluopyram, wherein the weight ratio of the pyrimidine nucleoside antibiotics to the fluopyram is (1-50): (50-1), the weight ratio of the pyrimidine nucleoside antibiotics to the fluopyram is preferably (1-20): (20-1), the weight ratio of the pyrimidine nucleoside antibiotics to the fluopyram is more preferably (1-10): (10-1), and the weight ratio of the pyrimidine nucleoside antibiotics to the fluopyram is most preferably 1: 1.
The sources of the pyrimidine nucleoside antibiotic and the fluopyram are not specially limited, and the conventional commercial products in the field can be adopted. The preparation method of the composition is not particularly limited, and the pyrimidine nucleoside antibiotic and the fluopyram are mixed by a conventional mixing method.
The bactericidal composition is applied in the amount of.
The bactericidal composition can be used for preventing and treating various plant diseases, and is particularly suitable for preventing and treating rice sheath blight disease, tomato gray mold and watermelon fusarium wilt. The composition is preferably mixed with water and then sprayed on crops, the spraying method is not particularly limited, and the composition can be sprayed by a conventional spraying method, wherein the dosage of the composition is 6-10.0 g/mu.
Compared with the prior art, the invention has the following beneficial effects:
1) compared with a single agent, the compound has obvious synergistic effect, and the control effect is improved;
2) the compounding of the medicament reduces the dosage, reduces the cost and lightens the pollution to the environment;
3) has better control effect on rice sheath blight, tomato gray mold and watermelon fusarium wilt, and has wide application range.
Detailed Description
In order to make the technical problems, solutions and advantages to be solved by the embodiments of the present invention clearer, specific embodiments will be described in detail below, but the present invention is by no means limited to these examples. The following description is only a preferred embodiment of the present invention, and is only for the purpose of explaining the present invention, and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the claims.
The combination of the two effective components in the composition, namely the pyrimidine nucleoside antibiotic and the fluopyram, has obvious synergistic effect on rice sheath blight disease, tomato gray mold and watermelon fusarium wilt, and the effect is not only simple superposition of the effects of the two medicaments, which can be clearly understood from the following test results.
Example 1
And (3) determining the indoor toxicity of the pyrimidine nucleoside antibiotics and fluopyram in the rice sheath blight.
Wherein, the rice sheath blight germ is collected in the field and purified and cultured indoors, and the test method refers to agricultural industry standard NY/T1156.2-2006 of the people's republic of China.
The results of the indoor toxicity test of the composition containing pyrimidine nucleoside antibiotics and fluopyram on rice sheath blight disease are shown in Table 1
TABLE 1 indoor toxicity test table for rice sheath blight pathogenic bacteria compounded by pyrimidine nucleoside antibiotics and fluopyram
| Medicament | EC50（mg/L） | Measured virulence index | Theoretical coefficient of merit | Co-toxicity coefficient |
| Pyrimidine nucleoside antibiotics (A) | 17.20 | 100 | - | - |
| Fluopyram (B) | 14.13 | 121.73 | - | - |
| A：B=50:1 | 14.09 | 122.07 | 100.43 | 121.55 |
| A：B=30:1 | 13.71 | 125.46 | 100.70 | 124.59 |
| A：B=20:1 | 13.07 | 131.60 | 101.04 | 130.25 |
| A：B=10:1 | 12.52 | 137.38 | 101.98 | 134.71 |
| A：B=1:1 | 11.47 | 149.96 | 110.87 | 135.26 |
| A：B=1:10 | 11.65 | 147.64 | 119.75 | 123.29 |
| A：B=1:20 | 11.29 | 152.35 | 120.70 | 126.22 |
| A：B=1:30 | 11.26 | 152.75 | 121.03 | 126.21 |
| A：B=1:50 | 11.48 | 149.83 | 121.30 | 123.52 |
As shown in Table 1, EC of pyrimidine nucleoside antibiotic and fluopyram against rice sheath blight disease50The synergistic effect is shown when the mass ratio of the pyrimidine nucleoside antibiotic to the fluopyram is 1:50-50:1, the cotoxicity coefficient is larger than 120, and the synergistic effect is best when the mass ratio of the pyrimidine nucleoside antibiotic to the fluopyram is 1: 1.
Example 2
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 50:1, a field test for preventing and treating the rice sheath blight is carried out, the pesticide application mode adopts spraying, the key point is that the stem base is arranged, 60 kilograms of pesticide is prepared according to the dosage in the following table per mu, the pesticide is applied for the first time in the rice tillering vigorous stage, the pesticide is applied for the second time after 7 days, the pesticide application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the pesticide application, the pesticide effect is calculated, and the result is shown in table 2.
TABLE 2 comparison table of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 90.3 | 87.3 | 85.4 |
| Pyrimidine nucleoside antibiotics | 11.0 | 73.2 | 70.8 | 65.2 |
| Fluopyram | 12.0 | 72.6. | 66.5 | 63.2 |
| Validamycin | 20.0 | 63.1 | 55.8 | 50.7 |
(data from 2020 field efficacy test)
As can be seen from Table 2, the results of the field efficacy tests show that, under the test conditions of example 2, the composition of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticides and reduce the cost.
Example 3
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 30:1, a field test for preventing and treating the rice sheath blight is carried out, the pesticide application mode adopts spraying, the key point is that the stem base is arranged, 60 kilograms of pesticide is prepared according to the dosage in the following table per mu, the pesticide is applied for the first time in the rice tillering vigorous stage, the pesticide is applied for the second time after 7 days, the pesticide application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the pesticide application, the pesticide effect is calculated, and the result is shown in table 3.
TABLE 3 comparison of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 92.5 | 89.7 | 86.4 |
| Pyrimidine nucleoside antibiotics | 11.0 | 71.6 | 68.1 | 63.2 |
| Fluopyram | 12.0 | 74.9 | 70.1 | 68.5 |
| Validamycin | 20.0 | 63.7 | 55.3 | 49.6 |
(data from 2020 field efficacy test)
As can be seen from table 3, the field efficacy test results show that, under the test conditions of example 3, the composition of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 4
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 20:1, a field test for preventing and treating the rice sheath blight is carried out, the pesticide application mode adopts spraying, the key point is at the stem base, 60 kilograms of pesticide is prepared according to the dosage in each mu according to the following table, the pesticide is applied for the first time in the full tillering period of the rice, the pesticide is applied for the second time after 7 days, the pesticide application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the pesticide application, the pesticide effect is calculated, and the result is shown in table 4.
TABLE 4 comparison table of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 91.8 | 89.2 | 86.6 |
| Pyrimidine nucleoside antibiotics | 11.0 | 73.1 | 65.7 | 60.2 |
| Fluopyram | 12.0 | 73.6 | 70.4 | 68.7 |
| Validamycin | 20.0 | 63.4 | 55.3 | 48.5 |
(data from 2020 field efficacy test)
As can be seen from table 4, the field efficacy test results show that, under the test conditions of example 4, the composition of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 5
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 10:1, a field test for preventing and treating the rice sheath blight is carried out, the pesticide application mode adopts spraying, the key point is at the stem base, 60 kilograms of pesticide is prepared according to the dosage in each mu according to the table below, the pesticide is applied for the first time in the full tillering period of the rice, the pesticide is applied for the second time after 7 days, the pesticide application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the pesticide application, the pesticide effect is calculated, and the result is shown in table 5.
TABLE 5 comparison table of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 92.2 | 90.1 | 88.6 |
| Pyrimidine nucleoside antibiotics | 11.0 | 68.8 | 60.7 | 58.0 |
| Fluopyram | 12.0 | 75.0 | 70.2 | 65.3 |
| Validamycin | 20.0 | 62.1 | 58.2 | 56.6 |
(data from 2020 field efficacy test)
As can be seen from table 5, the field efficacy test results show that, under the test conditions of example 5, the composition of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 6
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:1, a field test for preventing and treating the rice sheath blight is carried out, the pesticide application mode adopts spraying, the key point is that the pesticide is applied to the stem base part, 60 kilograms of pesticide is prepared according to the dosage of the following table per mu, the pesticide is applied for the first time in the full tillering stage of the rice, the pesticide is applied for the second time after 7 days, the pesticide application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the pesticide application, the pesticide effect is calculated, and the result is shown in table 6.
TABLE 6 comparison of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 96.0 | 94.6 | 90.3 |
| Pyrimidine nucleoside antibiotics | 11.0 | 70.2 | 66.7 | 60.3 |
| Fluopyram | 12.0 | 71.8 | 65.0 | 58.8 |
| Validamycin | 20.0 | 63.6 | 52.4 | 47.9 |
(data from 2020 field efficacy test)
As can be seen from table 6, the field efficacy test results show that, under the test conditions of example 6, the composition of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 7
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:10, a field test for preventing and treating the rice sheath blight is carried out, the pesticide application mode adopts spraying, the key point is at the stem base, 60 kilograms of pesticide is prepared according to the dosage in each mu according to the table below, the pesticide is applied for the first time in the rice tillering vigorous stage and is applied for the second time after 7 days, the pesticide application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the pesticide application, the pesticide effect is calculated, and the result is shown in the table 7.
TABLE 7 comparison of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 93.6 | 88.7 | 86.4 |
| Pyrimidine nucleoside antibiotics | 11.0 | 75.5 | 70.1 | 63.7 |
| Fluopyram | 12.0 | 77.8 | 70.5 | 68.1 |
| Validamycin | 20.0 | 63.2 | 60.8 | 55.6 |
(data from 2020 field efficacy test)
As can be seen from Table 7, the results of the field efficacy tests show that, under the test conditions of example 7, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 8
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:20, the field test of preventing and controlling the rice sheath blight is carried out, the pesticide application mode adopts spraying, the key point is at the stem base, 60 kilograms of pesticide is prepared according to the dosage in each mu according to the table below, the pesticide is applied for the first time in the rice tillering vigorous stage, the pesticide is applied for the second time after 7 days, the pesticide application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the pesticide application, the pesticide effect is calculated, and the result is shown in table 8.
TABLE 8 comparison table of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 93.2 | 90.7 | 88.0 |
| Pyrimidine nucleoside antibiotics | 11.0 | 66.8 | 62.7 | 58.1 |
| Fluopyram | 12.0 | 71.0 | 65.3 | 60.4 |
| Validamycin | 20.0 | 65.3 | 61.6 | 57.4 |
(data from 2020 field efficacy test)
As can be seen from Table 8, the results of the field efficacy tests show that, under the test conditions of example 8, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight disease, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 9
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:30, the spraying mode is adopted, the spraying mode is mainly adopted at the stem base, the dosage of each mu is prepared into 60 kg for application according to the following table dosage, the first application is carried out in the tillering stage of rice, the second application is carried out after 7 days, the application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the application, the drug effect is calculated, and the result is shown in table 9.
TABLE 9 comparison table of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 91.3 | 89.7 | 86.5 |
| Pyrimidine nucleoside antibiotics | 11.0 | 63.5 | 58.6 | 55.2 |
| Fluopyram | 12.0 | 71.8 | 64.8 | 60.4 |
| Validamycin | 20.0 | 63.2 | 59.1 | 57.8 |
(data from 2020 field efficacy test)
As can be seen from table 9, the results of the field efficacy tests show that, under the test conditions of example 9, the composition of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticides and reduce the cost.
Example 10
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:50, the field test of preventing and controlling the rice sheath blight is carried out, the pesticide application mode adopts spraying, the key point is at the stem base, 60 kilograms of pesticide is prepared according to the dosage in each mu according to the table below, the pesticide is applied for the first time in the rice tillering vigorous stage, the pesticide is applied for the second time after 7 days, the pesticide application is carried out twice, the disease index is measured 7 days, 14 days and 30 days after the pesticide application, the pesticide effect is calculated, and the result is shown in the table 10.
TABLE 10 comparison of the efficacy of controlling rice sheath blight
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 30 days (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 10.0 | 90.2 | 88.5 | 87.2 |
| Pyrimidine nucleoside antibiotics | 11.0 | 70.8 | 66.7 | 60.0 |
| Fluopyram | 12.0 | 73.1 | 68.9 | 60.3 |
| Validamycin | 20.0 | 63.2 | 57.2 | 54.4 |
(data from 2020 field efficacy test)
As can be seen from table 10, the field efficacy test results show that, under the test conditions of example 10, the composition of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on rice sheath blight disease, has synergistic effect in control effect compared with single dose, and can reduce the usage amount of pesticide and reduce cost.
Example 11
And (3) carrying out indoor toxicity determination on tomato gray mold by compounding pyrimidine nucleoside antibiotics and fluopyram.
The botrytis cinerea is collected in the field and is subjected to indoor purification culture, and the test method refers to agricultural industry standard NY/T1156.2-2006 of the people's republic of China.
The results of indoor toxicity tests on tomato gray mold using a composition containing pyrimidine nucleoside antibiotics and fluopyram are shown in Table 11.
TABLE 11 indoor toxicity assay table for Botrytis cinerea pathogens compounded by pyrimidine nucleoside antibiotics and fluopyram.
| Medicament | EC50（mg/L） | Actually measured toxicityForce index | Theoretical coefficient of merit | Co-toxicity coefficient |
| Pyrimidine nucleoside antibiotics (A) | 12.78 | 100 | - | - |
| Fluopyram (B) | 8.93 | 143.11 | - | - |
| A：B=50:1 | 10.51 | 121.60 | 100.85 | 120.58 |
| A：B=30:1 | 10.25 | 124.68 | 101.39 | 122.97 |
| A：B=20:1 | 10.12 | 126.28 | 102.53 | 123.74 |
| A：B=10:1 | 9.62 | 132.85 | 103.92 | 127.84 |
| A：B=1:1 | 7.88 | 162.18 | 121.56 | 133.42 |
| A：B=1:10 | 7.19 | 177.75 | 139.19 | 127.70 |
| A：B=1:20 | 7.16 | 178.49 | 141.06 | 126.54 |
| A：B=1:30 | 7.11 | 179.95 | 141.72 | 126.83 |
| A：B=1:50 | 7.30 | 175.07 | 142.26 | 123.06 |
As can be seen from Table 11, the pyrimidine nucleoside antibiotic, fluopyram, is active against Botrytis cinereaEC of disease50The synergistic effect is shown when the mass ratio of the pyrimidine nucleoside antibiotic to the fluopyram is 1:50-50:1, the cotoxicity coefficient is greater than 120, and the synergistic effect is best when the mass ratio of the pyrimidine nucleoside antibiotic to the fluopyram is 1: 1.
Example 12
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 50:1, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 12.
TABLE 12 comparison of the efficacy of controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 90.3 | 88.7 | 91.4 |
| Pyrimidine nucleoside antibiotics | 8.0 | 73.2 | 70.8 | 71.5 |
| Fluopyram | 8.0 | 78.6 | 76.5 | 77.9 |
| Carbendazim | 20.0 | 63.1 | 62.8 | 60.7 |
(data from 2020 field efficacy test)
As can be seen from table 12, the results of the field efficacy test show that, under the test conditions of example 12, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on the botrytis cinerea, has synergistic effect compared with a single agent in the control effect, and can reduce the usage amount of the pesticide and reduce the cost.
Example 13
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 30:1, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 13.
TABLE 13 comparison of the efficacy of controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 92.5 | 92.7 | 91.4 |
| Pyrimidine nucleoside antibiotics | 8.0 | 71.6 | 70.1 | 73.0 |
| Fluopyram | 8.0 | 74.9 | 75.1 | 75.5 |
| Carbendazim | 20.0 | 63.7 | 64.3 | 63.8 |
(data from 2020 field efficacy test)
As can be seen from table 13, the results of the field efficacy tests show that, under the test conditions of example 13, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on the botrytis cinerea, has synergistic effect compared with a single agent in the control effect, and can reduce the usage amount of the pesticide and reduce the cost.
Example 14
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 20:1, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 14.
TABLE 14 comparison of the efficacy of controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 91.8 | 92.2 | 91.6 |
| Pyrimidine nucleoside antibiotics | 8.0 | 73.1 | 72.7 | 73.4 |
| Fluopyram | 8.0 | 73.6 | 74.4 | 74.7 |
| Carbendazim | 20.0 | 63.4 | 65.0 | 63.5 |
(data from 2020 field efficacy test)
As can be seen from table 14, the field efficacy test results show that, under the test conditions of example 14, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on tomato gray mold, has synergistic effect in control effect compared with single dose, and can reduce the usage amount of pesticide and reduce the cost.
Example 15
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 10:1, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 15.
TABLE 15 comparison of the efficacy of controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 92.2 | 93.1 | 93.6 |
| Pyrimidine nucleoside antibiotics | 11.0 | 68.8 | 68.1 | 68.0 |
| Fluopyram | 12.0 | 73.0 | 72.2 | 72.3 |
| Carbendazim | 20.0 | 62.1 | 63.2 | 62.6 |
(data from 2020 field efficacy test)
As can be seen from table 15, the field efficacy test results show that, under the test conditions of example 15, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on tomato gray mold, has synergistic effect in control effect compared with single dose, and can reduce the usage amount of pesticide and reduce the cost.
Example 16
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 1:1, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 16.
TABLE 16 comparison of the drug effects for controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 96.0 | 96.5 | 97.3 |
| Pyrimidine nucleoside antibiotics | 8.0 | 70.2 | 71.7 | 71.3 |
| Fluopyram | 8.0 | 72.8 | 73.3 | 72.4 |
| Carbendazim | 20.0 | 63.6 | 62.4 | 61.9 |
(data from 2020 field efficacy test)
As can be seen from table 16, the field efficacy test results show that, under the test conditions of example 16, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on tomato gray mold, has synergistic effect in control effect compared with single dose, and can reduce the usage amount of pesticide and reduce the cost.
Example 17
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 1:10, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 17.
TABLE 17 comparison of the efficacy of controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 93.6 | 92.7 | 93.4 |
| Pyrimidine nucleoside antibiotics | 8.0 | 70.5 | 71.1 | 70.7 |
| Fluopyram | 8.0 | 72.8 | 71.5 | 70.1 |
| Carbendazim | 20.0 | 63.2 | 60.8 | 63.6 |
(data from 2020 field efficacy test)
As can be seen from table 17, the field efficacy test results show that, under the test conditions of example 17, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on tomato gray mold, has synergistic effect in control effect compared with single dose, and can reduce the usage amount of pesticide and reduce the cost.
Example 18
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 1:20, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 18.
TABLE 18 comparison of the efficacy of controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 93.2 | 93.7 | 93.0 |
| Pyrimidine nucleoside antibiotics | 8.0 | 70.8 | 72.7 | 69.1 |
| Fluopyram | 8.0 | 71.0 | 73.3 | 72.4 |
| Carbendazim | 20.0 | 62.3 | 63.6 | 63.4 |
(data from 2020 field efficacy test)
As can be seen from table 18, the results of the field efficacy tests show that, under the test conditions of example 18, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on tomato gray mold, has synergistic effect in control effect compared with single dose, and can reduce the usage amount of pesticide and reduce the cost.
Example 19
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 1:30, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 19.
TABLE 19 comparison of the efficacy of controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 91.3 | 90.7 | 90.5 |
| Pyrimidine nucleoside antibiotics | 8.0 | 69.9 | 70.7 | 70.4 |
| Fluopyram | 8.0 | 71.8 | 71.0 | 70.9 |
| Carbendazim | 20.0 | 63.2 | 63.1 | 62.8 |
(data from 2020 field efficacy test)
As can be seen from table 19, the field efficacy test results show that, under the test conditions of example 19, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on tomato gray mold, has synergistic effect in control effect compared with single dose, and can reduce the usage amount of pesticide and reduce the cost.
Example 20
Compounding pyrimidine nucleoside antibiotics and fluopyram according to the weight ratio of 1:50, spraying plants infected with tomato gray mold, wherein the area of a small area is 15m3Repeating the above steps for 3 times, investigating disease indexes 10 days after drug application, and calculating prevention effects, wherein the results are shown in Table 20.
TABLE 20 comparison of the efficacy of controlling tomato gray mold
| Medicament | Dosage (g/mu) of effective component | Control effect 1 (%) | Control effect 2 (%) | Control effect 3 (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 6.0 | 90.2 | 89.5 | 89.2 |
| Pyrimidine nucleoside antibiotics | 8.0 | 70.8 | 69.7 | 70.0 |
| Fluopyram | 8.0 | 73.1 | 71.9 | 72.3 |
| Carbendazim | 20.0 | 63.2 | 63.7 | 63.4 |
(data from 2020 field efficacy test)
As can be seen from table 20, the results of the field efficacy tests show that, under the test conditions of example 20, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on tomato gray mold, has synergistic effect compared with a single agent in control effect, and can reduce the usage amount of pesticide and reduce the cost.
Example 21
Indoor toxicity determination of watermelon fusarium wilt by compounding pyrimidine nucleoside antibiotics and fluopyram.
Wherein, watermelon fusarium wilt pathogen is collected in the field and is purified and cultured indoors, and the test method refers to the agricultural industry standard NY/T1156.2-2006 of the people's republic of China.
The results of the indoor toxicity test of the composition containing pyrimidine nucleoside antibiotics and fluopyram on watermelon fusarium wilt are shown in table 21.
TABLE 21 indoor toxicity test table for watermelon wilt pathogenic bacteria compounded by pyrimidine nucleoside antibiotics and fluopyram
| Medicament | EC50（mg/L） | Measured virulence index | Theoretical coefficient of merit | Co-toxicity coefficient |
| Pyrimidine nucleoside antibiotics (A) | 14.28 | 100 | - | - |
| Fluopyram (B) | 12.63 | 113.06 | - | - |
| A：B=50:1 | 11.85 | 120.51 | 100.26 | 120.20 |
| A：B=30:1 | 11.78 | 121.22 | 100.42 | 120.71 |
| A：B=20:1 | 11.55 | 123.64 | 100.62 | 122.87 |
| A：B=10:1 | 11.06 | 129.11 | 101.19 | 127.60 |
| A：B=1:1 | 8.97 | 159.20 | 106.53 | 149.44 |
| A：B=1:10 | 9.42 | 151.59 | 111.87 | 135.50 |
| A：B=1:20 | 9.85 | 144.97 | 112.44 | 128.94 |
| A：B=1:30 | 10.09 | 141.53 | 112.64 | 125.65 |
| A：B=1:50 | 10.34 | 138.10 | 112.80 | 122.43 |
As shown in Table 21, EC of pyrimidine nucleoside antibiotic and fluopyram against watermelon fusarium wilt50The content of the pyrimidine nucleoside antibiotics and the content of the fluopyram are respectively 14.28mg/L and 12.63mg/L, when the mass ratio of the pyrimidine nucleoside antibiotics to the fluopyram is 1:50-50:1, the cotoxicity coefficient is greater than 120, the synergy is shown, and when the mass ratio of the pyrimidine nucleoside antibiotics to the fluopyram is 1:1, the cotoxicity coefficient is the largest, and the synergy effect is the best.
Example 22
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 50:1 for field test of watermelon fusarium wilt prevention and treatment, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the fruiting period of watermelons, the medicaments are applied for the second time after 7 days, the medicament application is performed twice, the disease indexes are measured 7 days, 14 days and 21 days after the medicament application, and the medicament effect is calculated, wherein the results are shown in the table 22.
TABLE 22 comparison of the drug effects of controlling watermelon fusarium wilt
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 21 day (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 8.0 | 88.3 | 85.3 | 83.4 |
| Pyrimidine nucleoside antibiotics | 10.0 | 73.6 | 69.8 | 65.1 |
| Fluopyram | 10.0 | 72.5 | 67.9 | 63.4 |
| Mancozeb | 20.0 | 63.0 | 54.6 | 49.7 |
(data from 2020 field efficacy test)
As can be seen from table 22, the field efficacy test results show that, under the test conditions of example 22, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on watermelon fusarium wilt, and the control effect has synergistic effect compared with that of a single agent, so that the usage amount of the pesticide can be reduced, and the cost can be reduced.
Example 23
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 30:1, the compound is used for field test of watermelon fusarium wilt prevention and control, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the first medicament is applied in the fruiting period of the watermelon, the second medicament is applied after 7 days, the two times of medicament application are carried out, the disease indexes are measured 7 days, 14 days and 21 days after the medicament application, the medicament effect is calculated, and the result is shown in the table 23.
TABLE 23 comparison of the drug effects of controlling watermelon fusarium wilt
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 21 day (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 8.0 | 91.5 | 88.9 | 86.1 |
| Pyrimidine nucleoside antibiotics | 10.0 | 71.0 | 67.8 | 63.3 |
| Fluopyram | 10.0 | 74.9 | 70.1 | 68.5 |
| Mancozeb | 20.0 | 63.2 | 55.3 | 48.6 |
(data from 2020 field efficacy test)
As can be seen from table 23, the results of the field efficacy test show that, under the test conditions of example 23, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on watermelon fusarium wilt, and the control effect has synergistic effect compared with that of a single agent, and can reduce the usage amount of the pesticide and reduce the cost.
Example 24
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 20:1, field tests for controlling watermelon fusarium wilt are carried out, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the fruiting period of watermelon, the medicaments are applied for the second time after 7 days, the administration is carried out twice, disease indexes are measured 7 days, 14 days and 21 days after the administration, the drug effect is calculated, and the results are shown in the table 24.
TABLE 24 comparison of the drug effects of controlling watermelon fusarium wilt
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 21 day (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 8.0 | 91.6 | 89.1 | 85.4 |
| Pyrimidine nucleoside antibiotics | 10.0 | 73.2 | 65.9 | 59.2 |
| Fluopyram | 10.0 | 70.6 | 68.3 | 65.4 |
| Mancozeb | 20.0 | 63.2 | 55.7 | 48.2 |
(data from 2020 field efficacy test)
As can be seen from table 24, the results of the field efficacy tests show that, under the test conditions of example 24, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on watermelon fusarium wilt, and the control effect has synergistic effect compared with that of a single agent, so that the usage amount of the pesticide can be reduced, and the cost can be reduced.
Example 25
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 10:1, field tests for controlling watermelon fusarium wilt are carried out, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the fruiting period of watermelon, the medicaments are applied for the second time after 7 days, the administration is carried out twice, disease indexes are measured 7 days, 14 days and 21 days after the administration, the drug effect is calculated, and the results are shown in the table 25.
TABLE 25 comparison of the drug effects for controlling watermelon fusarium wilt
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 21 day (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 8.0 | 92.4 | 90.7 | 88.3 |
| Pyrimidine nucleoside antibiotics | 10.0 | 68.6 | 60.5 | 58.9 |
| Fluopyram | 10.0 | 75.2 | 70.4 | 65.8 |
| Mancozeb | 20.0 | 63.7 | 59.0 | 56.3 |
(data from 2020 field efficacy test)
As can be seen from table 25, the field efficacy test results show that, under the test conditions of example 25, the combination of pyrimidine nucleoside antibiotics and fluopyram has excellent control effect on watermelon fusarium wilt, and the control effect has synergistic effect compared with that of a single agent, so that the usage amount of pesticides can be reduced, and the cost is reduced.
Example 26
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:1, field tests for controlling watermelon fusarium wilt are carried out, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the fruiting period of watermelon, the medicaments are applied for the second time after 7 days, the administration is carried out twice, disease indexes are measured 7 days, 14 days and 21 days after the administration, the medicament effect is calculated, and the calculated medicament effect result is shown in the table 26.
TABLE 26 comparison of the drug action for preventing and treating watermelon fusarium wilt
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 21 day (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 8.0 | 96.5 | 94.8 | 92.6 |
| Pyrimidine nucleoside antibiotics | 10.0 | 70.2 | 66.7 | 61.3 |
| Fluopyram | 10.0 | 71.6 | 64.0 | 58.7 |
| Mancozeb | 20.0 | 63.3 | 52.0 | 48.9 |
(data from 2020 field efficacy test)
As can be seen from table 26, the results of the field efficacy test show that, under the test conditions of example 26, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on watermelon fusarium wilt, and the control effect has synergistic effect compared with that of a single agent, and can reduce the usage amount of the pesticide and reduce the cost.
Example 27
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:10, field tests for controlling watermelon fusarium wilt are carried out, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the fruiting period of watermelon, the medicaments are applied for the second time after 7 days, the administration is carried out twice, disease indexes are measured 7 days, 14 days and 21 days after the administration, the drug effect is calculated, and the results are shown in the table 27.
TABLE 27 comparison of the drug effect of controlling watermelon fusarium wilt
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 21 day (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 8.0 | 93.7 | 88.0 | 86.2 |
| Pyrimidine nucleoside antibiotics | 10.0 | 74.5 | 71.1 | 63.3 |
| Fluopyram | 10.0 | 77.6 | 70.4 | 68.2 |
| Mancozeb | 20.0 | 63.3 | 60.0 | 56.4 |
(data from 2020 field efficacy test)
As can be seen from table 27, the results of the field efficacy tests show that, under the test conditions of example 27, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on watermelon fusarium wilt, and the control effect has synergistic effect compared with that of a single agent, so that the usage amount of the pesticide can be reduced, and the cost can be reduced.
Example 28
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:20, field tests for controlling watermelon fusarium wilt are carried out, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the fruiting period of watermelon, the medicaments are applied for the second time after 7 days, the administration is carried out twice, disease indexes are measured 7 days, 14 days and 21 days after the administration, the drug effect is calculated, and the results are shown in the table 28.
TABLE 28 comparison of the drug action for preventing and treating watermelon fusarium wilt
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 21 day (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 8.0 | 92.3 | 91.7 | 87.9 |
| Pyrimidine nucleoside antibiotics | 10.0 | 66.1 | 62.5 | 58.4 |
| Fluopyram | 10.0 | 71.3 | 65.6 | 60.2 |
| Mancozeb | 20.0 | 65.0 | 60.8 | 56.2 |
(data from 2020 field efficacy test)
As can be seen from table 28, the results of the field efficacy test show that, under the test conditions of example 28, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has an excellent control effect on watermelon fusarium wilt, and the control effect has a synergistic effect compared with a single agent, and can reduce the usage amount of the pesticide and reduce the cost.
Example 29
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:30, field tests for controlling watermelon fusarium wilt are carried out, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the fruiting period of watermelon, the medicaments are applied for the second time after 7 days, the administration is carried out twice, disease indexes are measured 7 days, 14 days and 21 days after the administration, the drug effect is calculated, and the results are shown in the table 29.
TABLE 29 comparison of the drug effects for preventing and treating watermelon fusarium wilt
| Medicament | Dosage (g/mu) of effective component | 7 days (%) | 14 days (%) | 21 day (%) |
| Pyrimidine nucleoside antibiotic + fluopyram | 8.0 | 91.8 | 90.1 | 87.6 |
| Pyrimidine nucleoside antibiotics | 10.0 | 65.3 | 58.8 | 55.0 |
| Fluopyram | 10.0 | 71.4 | 63.8 | 61.1 |
| Mancozeb | 20.0 | 63.9 | 59.2 | 58.7 |
(data from 2020 field efficacy test)
As can be seen from table 29, the results of the field efficacy tests show that, under the test conditions of example 29, the combination of the pyrimidine nucleoside antibiotic and the fluopyram has excellent control effect on watermelon fusarium wilt, and the control effect has synergistic effect compared with that of a single agent, and can reduce the usage amount of the pesticide and reduce the cost.
Example 30
The pyrimidine nucleoside antibiotics and fluopyram are compounded according to the weight ratio of 1:50, field tests for controlling watermelon fusarium wilt are carried out, different medicaments are prepared into corresponding concentrations according to the requirements of the following table, the medicaments are applied for the first time in the fruiting period of watermelon, the medicaments are applied for the second time after 7 days, the administration is carried out twice, disease indexes are measured 7 days, 14 days and 21 days after the administration, the drug effect is calculated, and the results are shown in the table 30.
TABLE 30 comparison of the drug effect of controlling watermelon wilt
(data from 2020 field efficacy test)
As can be seen from table 30, the field efficacy test results show that, under the test conditions of example 30, the combination of pyrimidine nucleoside antibiotics and fluopyram has excellent control effect on watermelon fusarium wilt, and the control effect has synergistic effect compared with that of a single agent, so that the usage amount of pesticides can be reduced, and the cost is reduced.
The invention can be obtained by the above embodiments, the pyrimidine nucleoside antibiotic and fluopyram are mixed to have obvious synergistic effect, the drug effect can be greatly improved, and the pesticide use amount and the drug cost can be reduced.
Claims (7)
1. The bactericidal composition is characterized in that the effective components of the bactericidal composition consist of pyrimidine nucleoside antibiotics and fluopyram, and the weight ratio of the pyrimidine nucleoside antibiotics to the fluopyram is (1-50) to (50-1).
2. The bactericidal composition of claim 1, wherein the weight ratio of the pyrimidine nucleoside antibiotic to the fluopyram is (1-20): (20-1).
3. The bactericidal composition of claim 1, wherein the weight ratio of the pyrimidine nucleoside antibiotic to the fluopyram is (1-10): (10-1).
4. The bactericidal composition of claim 1, wherein the pyrimidine nucleoside antibiotic and fluopyram are present in a weight ratio of 1: 1.
5. The bactericidal composition of claim 1, wherein the bactericidal composition is present in an amount ranging from 6 to 10.0 g/acre.
6. Use of a fungicidal composition according to any of claims 1 to 5 for the control of plant diseases.
7. The use of a fungicidal composition according to claim 6 for the control of rice sheath blight disease, tomato gray mold and/or watermelon fusarium wilt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011392983.XA CN112385669A (en) | 2020-12-03 | 2020-12-03 | Bactericidal composition and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011392983.XA CN112385669A (en) | 2020-12-03 | 2020-12-03 | Bactericidal composition and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112385669A true CN112385669A (en) | 2021-02-23 |
Family
ID=74605770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011392983.XA Pending CN112385669A (en) | 2020-12-03 | 2020-12-03 | Bactericidal composition and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112385669A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109953027A (en) * | 2017-12-22 | 2019-07-02 | 东莞东阳光科研发有限公司 | A kind of bactericidal composition of fluorine-containing phenylate amide and antibiotic in pyrimidine nucleoside class |
-
2020
- 2020-12-03 CN CN202011392983.XA patent/CN112385669A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109953027A (en) * | 2017-12-22 | 2019-07-02 | 东莞东阳光科研发有限公司 | A kind of bactericidal composition of fluorine-containing phenylate amide and antibiotic in pyrimidine nucleoside class |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110583716A (en) | Pesticide composition containing brassinolide compound, chitosan and oligosaccharins and application thereof | |
| CN109805019B (en) | Fungicidal composition and use thereof | |
| CN106614604A (en) | Sterilizing and disease preventing suspended seed coating and application | |
| CN112385669A (en) | Bactericidal composition and application thereof | |
| EP1419693B1 (en) | Composition for controlling plant pathogenic bacterium and method of controlling plant pathogenic bacterium | |
| CN103651492A (en) | Fungicidal composition containing iprodione and triclopyricarb and application thereof | |
| CN110731337B (en) | Composition for preventing and treating plant diseases and application thereof | |
| CN107372550A (en) | A kind of pesticide combination comprising folpet and benzo alkene fluorine bacterium azoles | |
| CN106508937A (en) | Bactericidal composition containing fenhexamid and Zhongshengmycin as well as application of bactericidal composition | |
| CN108522544B (en) | Validamycin-containing bactericidal composition and application thereof | |
| CN114532346B (en) | Fungicidal and bacterial composition and application thereof | |
| CN108990992B (en) | Sterilization composition containing pyraoxystrobin and mancozeb | |
| CN112189663B (en) | Fungicidal and bacterial composition and application | |
| CN112189665B (en) | Fungicidal bacterium composition and application | |
| CN112189669B (en) | Fungicidal and bacterial compositions and uses | |
| CN103651453A (en) | Bactericidal composition containing hymexazol and carbendazim and application of bactericidal composition | |
| CN109169711B (en) | Bactericidal composition containing shenqinmycin and pyriofenone | |
| CN116391715B (en) | Plant growth regulator composition and application thereof | |
| CN116019116B (en) | Pesticide powder and application method thereof | |
| CN111466392B (en) | Bactericidal composition containing benziothiazolinone and tetraconazole and application thereof | |
| CN104322521B (en) | A kind of combination sterilization composition containing fluazinam | |
| CN111670902B (en) | Pesticide composition containing Vanilthioketal and ethylicin and application thereof | |
| JP2877155B2 (en) | Agricultural and horticultural fungicides | |
| JPH04128204A (en) | Agricultural and horticultural germicide | |
| CN1164174C (en) | Agricultural chemical containing thia- and oxa-components |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210223 |
|
| RJ01 | Rejection of invention patent application after publication |