CN112375704A - Microbial agent for degrading kitchen waste and application method thereof - Google Patents

Microbial agent for degrading kitchen waste and application method thereof Download PDF

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Publication number
CN112375704A
CN112375704A CN202011261738.5A CN202011261738A CN112375704A CN 112375704 A CN112375704 A CN 112375704A CN 202011261738 A CN202011261738 A CN 202011261738A CN 112375704 A CN112375704 A CN 112375704A
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China
Prior art keywords
kitchen waste
garbage
degrading
microbial agent
kitchen
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CN202011261738.5A
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Chinese (zh)
Inventor
李力
张建东
刘一澎
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Yangzhou Jinwei Environmental Protection Technology Co Ltd
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Yangzhou Xinwei Environmental Protection Biotechnology Co ltd
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Priority to CN202011261738.5A priority Critical patent/CN112375704A/en
Publication of CN112375704A publication Critical patent/CN112375704A/en
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09BDISPOSAL OF SOLID WASTE
    • B09B3/00Destroying solid waste or transforming solid waste into something useful or harmless

Abstract

The invention is applicable to the technical field of microbial agents, and provides a microbial agent for degrading kitchen waste and an application method thereof, wherein the microbial agent comprises the following preparation steps: step S100: selecting strains; step S200: culturing strains; step S300: the invention solves the problems that the treatment capacity of the kitchen waste is larger and larger along with the improvement of living standard, but the existing biological treatment method is mostly one-time degradation, the degradation period is slow and the degradation efficiency is low.

Description

Microbial agent for degrading kitchen waste and application method thereof
Technical Field
The invention belongs to the field of microbial agents, and particularly relates to a microbial agent for degrading kitchen waste and an application method thereof.
Background
The kitchen waste is food residue produced in catering industries such as families, dining halls, hotels and the like, the proportion of the food residue in the municipal waste is up to 50%, the content of organic matters can reach more than 85%, the food waste is easy to decay, ferment and generate stink, the water content is high, the food waste is inconvenient to transport, and secondary pollutants such as leachate and the like are easily generated due to improper treatment.
The environment-friendly treatment of the kitchen waste in China is still in a primary stage, at present, the treatment modes of the kitchen waste mainly comprise a crushing method, a landfill method and a biological treatment method, the biological treatment method has obvious environment-friendly benefit, does not generate secondary pollution, and can effectively realize the resource utilization of waste, which is a hotspot of current research.
Along with the improvement of living standard, the treatment capacity of the kitchen waste is larger and larger, however, the existing biological treatment method is mostly one-time degradation, the degradation period is slow, and the degradation efficiency is not high.
Disclosure of Invention
The invention provides a microbial agent for degrading kitchen waste and an application method thereof, and aims to solve the problems that the kitchen waste treatment capacity is larger and larger along with the improvement of living standard, but the existing biological treatment method is mostly one-time degradation, the degradation period is slow, and the degradation efficiency is low.
The invention is realized in such a way that a microbial agent for degrading kitchen waste comprises the following preparation steps:
step S100: selecting strains;
step S200: culturing strains;
step S300: and (4) mixing strains.
Preferably, in step S100, the strain is bacillus licheniformis.
Preferably, in step S200, the parameters of strain culture: the temperature is 30-35 ℃, and the culture medium is respectively a fiber culture medium, a starch culture medium, a protein culture medium and a fat culture medium.
Preferably, in step S300, the initial mixing ratio of the microbial inoculum is 1: 1: 1: 1.
the application method of the microbial agent for degrading the kitchen waste comprises a waste treatment device and comprises the following steps:
step T100: feeding garbage;
step T200: separating and treating garbage;
step T300: adding a microbial inoculum;
step T400: and (4) cleaning residues.
Preferably, in the step T100, the feeding of the garbage specifically includes sorting the kitchen garbage and feeding the kitchen garbage into a garbage treatment device.
Preferably, in the step T200, the garbage disposal apparatus is divided into four treatment steps, and the kitchen garbage is subjected to a fiber degradation zone, a starch degradation zone, a protein degradation zone and a fat degradation zone, respectively.
Preferably, in the step T300, the mixing ratio of the bacterial strain in the microbial inoculum is adjusted according to the characteristics of the bacterial strain.
Preferably, in the step T400, the processing of the residue is specifically artificial burying.
Compared with the prior art, the invention has the beneficial effects that: according to the microbial agent for degrading the kitchen waste and the application method thereof, bacillus licheniformis with different resistance resistances is cultured according to different nutrient media, after the waste enters the waste treatment equipment, the treatment equipment is divided into a fiber degradation area, a starch degradation area, a protein degradation area and a fat degradation area, the resistant strains in different areas are more in proportion in the microbial agent, fibers, starch, proteins and fats in the kitchen waste can be rapidly degraded, the degradation efficiency is improved, and the problems that the treatment amount of the kitchen waste is larger and larger along with the improvement of the living standard are solved, however, most of the existing biological treatment methods are one-time degradation, the degradation period is slow, and the degradation efficiency is low.
Drawings
FIG. 1 is a flow chart of the preparation of the microbial inoculum according to the invention;
FIG. 2 is a flow chart of an application method of the present invention;
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The invention provides a technical scheme that: as shown in FIG. 1: a microbial agent for degrading kitchen waste comprises the following preparation steps:
step S100: selecting strains;
step S200: culturing strains;
step S300: and (4) mixing strains.
In the embodiment, the bacillus licheniformis can exist in a growth state, the bacillus licheniformis can adjust the dysbacteriosis to achieve the purpose of treatment, an organism can be promoted to generate an antibacterial active substance and kill pathogenic bacteria, the kitchen waste is effectively degraded, the growth of bacteria in the kitchen waste is inhibited, the bacillus licheniformis is respectively put into a fiber culture medium, a starch culture medium, a protein culture medium and a fat culture medium in the environment with the temperature of 30-35 ℃, after being cultured for a period of time, the bacillus licheniformis with resistance to fiber, starch, protein and fat can be obtained, and four strains with different resistance are cultured according to the proportion of 1: 1: 1: 1, forms the microbial inoculum, and then according to the degradation object of difference, improves the content of this resistant bacterial in the microbial inoculum, and then can carry out accurate degradation to kitchen garbage, improves degradation speed, makes the explanation more abundant.
Further, in step S100, the strain is bacillus licheniformis.
In the embodiment, the bacillus licheniformis can exist in a growth state, and can adjust the dysbacteriosis to achieve the purpose of treatment, so that the organism can be promoted to generate antibacterial active substances, pathogenic bacteria can be killed, the kitchen waste can be effectively degraded, and the growth of bacteria in the kitchen waste can be inhibited.
Further, in step S200, the parameters of strain culture: the temperature is 30-35 ℃, and the culture medium is respectively a fiber culture medium, a starch culture medium, a protein culture medium and a fat culture medium.
In the embodiment, in the environment with the temperature of 30-35 ℃, the bacillus licheniformis is respectively put into a fiber culture medium, a starch culture medium, a protein culture medium and a fat culture medium, and after a period of culture, the bacillus licheniformis with resistance to fiber, starch, protein and fat can be obtained.
Further, in step S300, the initial mixing ratio of the microbial inoculum is 1: 1: 1: 1.
in this embodiment, four species with different resistance are used in the following order of 1: 1: 1: 1 to form a microbial agent, and further increasing the content of the resistant strain in the microbial agent according to different degradation objects.
As shown in fig. 2: the application method of the microbial agent for degrading the kitchen waste comprises a waste treatment device and comprises the following steps:
step T100: feeding garbage;
step T200: separating and treating garbage;
step T300: adding a microbial inoculum;
step T400: and (4) cleaning residues.
In the embodiment, as the kitchen waste is often mixed with other waste and is difficult to degrade by microorganisms, the artificial waste is firstly sorted, the kitchen waste is sorted out from the waste, and the sorted kitchen waste is put into the waste treatment equipment, so that the waste treatment equipment can accurately degrade the kitchen waste, the waste firstly enters the fiber degradation area, the content of the fiber resistant strain in the microbial inoculum is adjusted, the high fiber waste in the kitchen waste can be fully degraded, the waste after primary degradation enters the starch degradation area, the content of the starch resistant strain in the microbial inoculum is adjusted, the starch waste in the kitchen waste can be degraded, and then the starch resistant strain enters the protein degradation area, the content of the protein resistant strain in the microbial inoculum is adjusted, the high protein in the kitchen waste can be degraded, and finally the waste enters the fat degradation area, the content of the fat-resistant strain in the microbial inoculum is adjusted, high-fat garbage in kitchen garbage can be fully degraded, the garbage can be degraded into carbon dioxide and organic matter residues quickly and accurately comprehensively, the degraded garbage is divided into the organic matter residues and the carbon dioxide, the carbon dioxide is directly discharged into the air after being detected to reach the standard, the organic matter residues are buried manually, the organic matter residues do not contain pollution, and the soil can be fertilized to recycle the garbage.
Further, in step T100, the feeding of the garbage specifically includes sorting the kitchen garbage and feeding the kitchen garbage into a garbage disposal device.
In this embodiment, because in the kitchen garbage, often thoughtlessly have all the other rubbish, be difficult to degrade through the microorganism, at first sort artifical rubbish, sort out kitchen garbage from rubbish, and then throw into refuse handling installation with the kitchen garbage of sorting out, the refuse handling installation of being convenient for is accurate degrades kitchen garbage.
Further, in step T200, the garbage disposal apparatus is divided into four treatment steps, and the kitchen garbage is subjected to a fiber degradation zone, a starch degradation zone, a protein degradation zone and a fat degradation zone, respectively.
In this embodiment, rubbish firstly gets into the fibre degradation district, can carry out full degradation to the high fibre rubbish in the rubbish from cooking, and then the rubbish that preliminary degradation was accomplished gets into starch degradation district, can degrade the starch rubbish in the rubbish from cooking, and then get into the protein degradation district, can degrade the high protein in the rubbish from cooking, gets into the fat degradation district at last, can carry out full degradation to the high fat rubbish in the rubbish from cooking, synthesizes and can be in quick and accurate degradation of rubbish into carbon dioxide and organic matter residue.
Further, in step T300, the mixing ratio of the strain in the microbial inoculum is adjusted according to the characteristics of the strain.
In this embodiment, when the garbage enters the fiber degradation zone, the content of the fiber resistant strain in the microbial inoculum is adjusted, the high fiber garbage can be rapidly degraded, when the garbage enters the starch degradation zone, the content of the starch resistant strain in the microbial inoculum is adjusted, the starch garbage can be rapidly degraded, when the garbage enters the protein degradation zone, the content of the protein resistant strain in the microbial inoculum is adjusted, the high protein garbage can be rapidly degraded, when the garbage enters the fat degradation zone, the content of the fat resistant strain in the microbial inoculum is adjusted, and the high fat garbage can be rapidly degraded.
Further, in step T400, the processing of the residue is specifically artificial burying.
In the embodiment, the degraded garbage is divided into organic matter residues and carbon dioxide, the carbon dioxide is directly discharged into the air after being detected to reach the standard, the organic matter residues are buried manually, the organic matter residues do not contain pollution, and the fertilizer can be applied to the land to recycle the garbage.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims (9)

1. A microbial agent for degrading kitchen waste is characterized in that: the preparation method comprises the following preparation steps:
step S100: selecting strains;
step S200: culturing strains;
step S300: and (4) mixing strains.
2. The microbial agent for degrading kitchen waste according to claim 1, wherein: in the step S100, the strain is Bacillus licheniformis.
3. The microbial agent for degrading kitchen waste according to claim 1, wherein: in step S200, the parameters of strain culture: the temperature is 30-35 ℃, and the culture medium is respectively a fiber culture medium, a starch culture medium, a protein culture medium and a fat culture medium.
4. The microbial agent for degrading kitchen waste according to claim 1, wherein: in the step S300, the initial mixing ratio of the microbial inoculum is 1: 1: 1: 1.
5. the application method of the microbial agent for degrading the kitchen waste comprises waste treatment equipment, and is characterized in that: the method comprises the following steps:
step T100: feeding garbage;
step T200: separating and treating garbage;
step T300: adding a microbial inoculum;
step T400: and (4) cleaning residues.
6. The application method of the microbial agent for degrading the kitchen waste, according to claim 5, is characterized in that: in the step T100, the garbage input is specifically sorting the kitchen garbage, and inputting the kitchen garbage into the garbage treatment equipment.
7. The application method of the microbial agent for degrading the kitchen waste, according to claim 5, is characterized in that: in the step T200, the garbage treatment equipment is divided into four treatment steps, and the kitchen garbage is subjected to a fiber degradation area, a starch degradation area, a protein degradation area and a fat degradation area respectively.
8. The application method of the microbial agent for degrading the kitchen waste, according to claim 5, is characterized in that: in the step T300, the mixing ratio of the strains in the microbial inoculum is adjusted according to the characteristics of the strains.
9. The application method of the microbial agent for degrading the kitchen waste, according to claim 1, is characterized in that: in the step T400, the processing of the residue is specifically artificial burying.
CN202011261738.5A 2020-11-12 2020-11-12 Microbial agent for degrading kitchen waste and application method thereof Withdrawn CN112375704A (en)

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Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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CN102586112A (en) * 2012-03-14 2012-07-18 田兴军 Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof
CN103756941A (en) * 2014-01-26 2014-04-30 北京绿色能量环境工程有限公司 Compound microbial agent as well as processing method and application thereof
CN104341180A (en) * 2014-10-22 2015-02-11 天津生态城环保有限公司 Method for producing organic fertilizer from kitchen waste
CN207592415U (en) * 2017-11-03 2018-07-10 深圳市阳光三环生态环境股份有限公司 Kitchen garbage purifies pulping system with rubbish from cooking
CN108676753A (en) * 2018-05-31 2018-10-19 深圳市微米生物技术有限公司 A kind of Geobacillus stearothermophilus and microbial inoculum and its application in handling kitchen garbage
CN108823119A (en) * 2018-05-31 2018-11-16 深圳市微米生物技术有限公司 A kind of bacillus amyloliquefaciens and microbial inoculum and its application in processing kitchen garbage
CN109422552A (en) * 2017-08-23 2019-03-05 连云港市连威农牧科技有限公司 The processing method and its tunning and processing method of a kind of kitchen garbage
CN109652322A (en) * 2018-11-16 2019-04-19 韶关市诚湃新能源科技有限公司 A kind of house refuse microbiological decomposer and preparation method thereof
CN110894477A (en) * 2019-09-18 2020-03-20 浙江工业大学 Compound microbial inoculum for degrading kitchen waste, application and kitchen waste degradation method
CN111154690A (en) * 2020-01-20 2020-05-15 上海创净生物环保科技有限公司 Geobacillus thermooleophilic bacillus, microbial inoculum thereof and application of bacillus thermooleophilic bacillus in kitchen waste treatment

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102553886A (en) * 2011-12-23 2012-07-11 天津生态城水务有限公司 Full-biomass resource utilization method for kitchen waste
CN102586112A (en) * 2012-03-14 2012-07-18 田兴军 Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof
CN103756941A (en) * 2014-01-26 2014-04-30 北京绿色能量环境工程有限公司 Compound microbial agent as well as processing method and application thereof
CN104341180A (en) * 2014-10-22 2015-02-11 天津生态城环保有限公司 Method for producing organic fertilizer from kitchen waste
CN109422552A (en) * 2017-08-23 2019-03-05 连云港市连威农牧科技有限公司 The processing method and its tunning and processing method of a kind of kitchen garbage
CN207592415U (en) * 2017-11-03 2018-07-10 深圳市阳光三环生态环境股份有限公司 Kitchen garbage purifies pulping system with rubbish from cooking
CN108676753A (en) * 2018-05-31 2018-10-19 深圳市微米生物技术有限公司 A kind of Geobacillus stearothermophilus and microbial inoculum and its application in handling kitchen garbage
CN108823119A (en) * 2018-05-31 2018-11-16 深圳市微米生物技术有限公司 A kind of bacillus amyloliquefaciens and microbial inoculum and its application in processing kitchen garbage
CN109652322A (en) * 2018-11-16 2019-04-19 韶关市诚湃新能源科技有限公司 A kind of house refuse microbiological decomposer and preparation method thereof
CN110894477A (en) * 2019-09-18 2020-03-20 浙江工业大学 Compound microbial inoculum for degrading kitchen waste, application and kitchen waste degradation method
CN111154690A (en) * 2020-01-20 2020-05-15 上海创净生物环保科技有限公司 Geobacillus thermooleophilic bacillus, microbial inoculum thereof and application of bacillus thermooleophilic bacillus in kitchen waste treatment

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Address after: No.50, xinganquan East Road, Yangzhou City, Jiangsu Province, 225000

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Application publication date: 20210219