CN112369508A - Compound antioxidant for feed and preparation method thereof - Google Patents

Abstract

The invention provides a compound antioxidant for feed and a preparation method thereof. The technical scheme screens components having potential influence on oxygen free radicals based on experimental means, and researches interaction and synergistic relationship between each component and ethoxyquinoline. Experiments show that the corn ferment prepared by the specific process can generate a synergistic effect on ethoxyquin, and the synergistic effect is more remarkable when sodium alginate, magnesium lauryl sulfate, sodium carboxymethyl starch, ammonium glycyrrhizinate, acesulfame potassium, sucrose ester, sodium caseinate and arbutin are further introduced. Based on the beneficial findings, the invention constructs a compound antioxidant formula by using the components and provides a preparation method thereof. Experiments prove that the compound scheme of the invention can play a role in an extremely low dosage, can obviously improve the oxidation resistance of grease, and also improves the protection of fat-soluble vitamins to a certain extent, thereby having outstanding technical advantages.

Description

Compound antioxidant for feed and preparation method thereof

Technical Field

The invention relates to the technical field of feed additives, in particular to a compound antioxidant for feed and a preparation method thereof.

Background

During the storage of the feed, not only the feed needs to be kept fresh, but also the nutritional ingredients in the feed should be ensured not to deteriorate so as to achieve the expected feeding effect. Fat, protein, carbohydrate and vitamins in the feed are in contact with oxygen in the air, and are easy to decay. The oxidized components interact with each other to accelerate the oxidation of various components, and finally, the grease in the feed is clamed, faded and browned, and toxic substances such as aldehyde, ketone and the like are even generated. In the process, fat-soluble vitamins and lutein in the feed are oxidized and damaged, so that protein and energy are lost, palatability is reduced, the quality of the feed is reduced if the palatability is reduced, animal growth and development are influenced, poisoning and even death accidents are caused if the palatability is reduced, and economic loss is caused to the breeding industry.

Thus, antioxidants have long been important feed additives. Many substances can be used as feed antioxidant, such as L-ascorbic acid, butylated hydroxytoluene, butylated hydroxyanisole, tocopherol, gallate, ethoxyquinoline, etc. Among them, ethoxyquinoline, di-tert-butylhydroxytoluene, tert-butylhydroxyanisole, and propyl gallate are mainly used widely.

Ethoxyquinoline, full name of chemical name: 6-ethoxy-2, 2, 4-trimethyl-1, 2-dihydroquine english letter: ethoxquin or EMQ abbreviation, the most commonly used foreign trade name: yamaquine (Santoquin), the most commonly used trade name in China: antioxidant quinoline. The product is the most widely used feed antioxidant variety at home and abroad at present, and has good effect, low price and safe use. However, a large number of tests prove that the antioxidant effect of the antioxidant quinoline on grease is not ideal, and the color of a feed product added with the antioxidant quinoline is gradually deepened in the storage process, so that the color of the feed is influenced by using the antioxidant quinoline in a large amount in the premix. Di-tert-butylhydroxytoluene, abbreviated as BHT; the stability is high, and the antioxidant effect is not affected when the composition is heated; however, studies show that BHT has certain toxic and side effects on human bodies, and particularly has adverse effects on livers and spleens; although it is not known whether BHT, which is a feed additive, is absorbed by the human body by eating meat, there is a certain potential risk. Tert-butyl hydroxyanisole, abbreviated as BHA; the antioxidant effect on grease is superior to BHT, and the antibacterial effect on grease is strong, but the price is high, and the antioxidant is rarely used in the feed industry due to cost consideration. Propyl gallate, abbreviated as PG; is difficult to dissolve in fat and water, and is easy to color, so the addition amount is not suitable to be too large, and the antioxidant is generally used together with other antioxidants. Moreover, PG is capable of chelating metal ions, which limits its range of applications to some extent.

In summary, ethoxyquin is still the best antioxidant for use in the feed industry. However, its application is limited because it has an undesirable antioxidant effect on fats and oils and a coloring effect on feeds.

Disclosure of Invention

The invention aims to provide a compound antioxidant for feed and a preparation method thereof aiming at overcoming the technical defects of the prior art, and aims to solve the technical problem that the antioxidant effect of ethoxyquin on grease needs to be improved.

The invention aims to solve another technical problem of how to provide a novel compound antioxidant which has better antioxidant effect, and is safe and stable.

In order to achieve the technical purpose, the invention adopts the following technical scheme:

a composite antioxidant for feed comprises the following components in parts by weight: 35-42 parts of ethoxyquinoline, 0.12-0.18 part of sodium alginate, 0.03-0.07 part of magnesium lauryl sulfate, 0.01-0.09 part of sodium carboxymethyl starch, 0.03-0.05 part of ammonium glycyrrhizinate, 0.02-0.06 part of acesulfame potassium, 0.07-0.13 part of sucrose ester, 0.05-0.07 part of sodium caseinate, 0.02-0.06 part of arbutin and 3.2-4.4 parts of corn ferment.

Preferably, the composite antioxidant for feed also comprises 0.06-0.08 weight part of glutathione.

Preferably, the composite antioxidant for feed also comprises 0.02-0.04 parts by weight of mannitol.

Preferably, the composite antioxidant for feed also comprises 0.01-0.03 weight part of glyceryl monostearate.

Preferably, the composite antioxidant for feed also comprises 0.08-0.12 weight part of xanthan gum.

Preferably, the compound antioxidant for the feed consists of the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.15 part of sodium alginate, 0.05 part of magnesium lauryl sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate, 0.04 part of arbutin and 3.8 parts of corn ferment.

Preferably, the compound antioxidant for the feed consists of the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.15 part of sodium alginate, 0.05 part of magnesium lauryl sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate, 0.04 part of arbutin, 3.8 parts of corn ferment, 0.07 part of glutathione, 0.03 part of mannitol, 0.02 part of glycerol monostearate and 0.1 part of xanthan gum.

On the basis of the technical scheme, the invention further provides a preparation method of the compound antioxidant for the feed, which comprises the following steps:

1) preparing an aqueous solution containing 0.27g/L of tea polyphenol, 0.74g/L of ethylene diamine tetraacetic acid and 0.96g/L of potassium citrate, soaking the corn cob cores in the aqueous solution, and continuously soaking for 48 hours at the temperature of 55 ℃; then discarding the liquid phase, crushing the wet rod core, centrifuging and taking the liquid phase to obtain a rod core leaching solution;

2) soaking corn stigma in BG-11 culture medium at normal temperature for 24h, draining, and pulverizing to obtain wet material; spreading the wet material on a tray to form a wet material layer with the thickness of 1-1.5 cm, and sprinkling trichoderma harzianum on the wet material layer according to the proportion of 20g of bacterial powder per kg of wet material; covering the upper end of the wet material layer with Trichoderma harzianum with corn flour in an amount which is 1.5 times the weight of the wet material; spraying lactobacillus delbrueckii on the corn powder layer according to the proportion of 15g of bacterial powder per kg of wet material; placing the tray at 33 ℃ under the condition of 1.2 atmospheric pressure, standing and fermenting for 72 h;

3) uniformly mixing all materials on a tray to obtain a fermented coarse material, mixing the rod core leaching solution obtained in the step 1) with the fermented coarse material with equal weight, adding water with the weight 6 times that of the fermented coarse material, uniformly mixing, and keeping for 1h under the ultrasonic oscillation condition at 47 ℃ to obtain a secondary fermentation substrate; inoculating Bacillus cereus to the secondary fermentation substrate to 10⁶～10⁷CFU/mL, Rhodococcus erythropolis to 10⁶～10⁷CFU/mL, Pichia to 5X 10⁶～5×10⁷CFU/mL, and carrying out aerobic fermentation for 36h at the temperature of 36 ℃; spreading the fermentation product, naturally drying in air, collecting the dried powder, and crushing to obtain corn enzyme with the granularity not less than 80 meshes;

4) uniformly mixing 3.2-4.4 parts by weight of the corn ferment obtained in the step 3) with 35-42 parts by weight of ethoxyquinoline, 0.12-0.18 part by weight of sodium alginate, 0.03-0.07 part by weight of magnesium lauryl sulfate, 0.01-0.09 part by weight of sodium carboxymethyl starch, 0.03-0.05 part by weight of ammonium glycyrrhizinate, 0.02-0.06 part by weight of acesulfame potassium, 0.07-0.13 part by weight of sucrose ester, 0.05-0.07 part by weight of sodium caseinate and 0.02-0.06 part by weight of arbutin to obtain the composite antioxidant for the feed.

In the technical scheme, the ethoxyquin is a conventional antioxidant, has the effect of preventing the rancidity of the polyunsaturated fatty acid in the feed, can protect fat-soluble vitamins and part of B vitamins in the feed from being oxidized, improves the utilization rate of amino acid in the feed, and reduces the daily ration energy value and the using amount of protein. Sodium alginate is a byproduct after extraction of iodine and mannitol from brown algae such as kelp or gulfweed, and its molecules are formed by connecting beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G). Based on its own viscosity, sodium alginate is mainly used as a thickener, stabilizer and emulsifier for food at present. Magnesium lauryl sulfate is a water-soluble lubricant and is mainly used as a pharmaceutical adjuvant at present. Sodium carboxymethyl starch is a modified starch etherified with carboxymethyl, which is tasteless, non-toxic, not easily mouldable, and easily soluble in water. The product can be used in different foods to achieve thickening, suspending, emulsifying, shape keeping, film forming, puffing, and acid resisting effects. Ammonium glycyrrhetate is mainly used as a medicament at present, is clinically used for treating cough and asthma and partial liver diseases, and has good safety under the condition of trace amount although the application in the field of food is not seen. Acesulfame potassium is a sweetener, is applied to cosmetics, foods, beverages, sweet seasonings, vitamins and pharmaceutical preparations, and has no report of oxidation resistance at present. Sucrose ester is also called fatty acid sucrose polyester, is a compound formed by dehydration condensation of sucrose and less than 8 fatty acid molecules under proper conditions, is mainly used as a nonionic surfactant at present, is harmless to human bodies, and can be completely biodegraded. Sodium caseinate is a sodium salt of casein, is mainly used as a thickening agent or an emulsifying agent at present, and also has good thermal stability and interface characteristics. Arbutin is mainly used as a raw material of cosmetics, burn and scald medicines and intestinal inflammation at present, and has certain anti-inflammation and whitening effects. The corn enzyme is a mixture obtained by fermenting corn serving as a substrate through microorganisms, has a mixed enzyme system, and is mainly used as a health-care food for drinking at present; because the nutrients, the flora and the fermentation conditions adopted in the fermentation are different, the corn ferment prepared by different processes has different components and properties, and the corn ferment adopted by the invention is prepared by the specific process of the invention. No correlation between ferment and ethoxyquinoline in oxidation resistance is found in the prior art.

In summary, the ingredients in the formula of the present invention, except ethoxyquinoline, have no antioxidant activity in the current cognitive range, and do not show a synergistic relationship, and the application fields and the functions thereof are different. The research of the invention finds that after the components are mixed according to a specific proportion, the synergistic effect on the ethoxyquin can be achieved, so that the antioxidant effect on the grease is obviously improved, the compounded components can play a role in an extremely low dosage, the safety risk is avoided, the cost can be controlled, and the positive significance on improving the efficacy of the ethoxyquin is achieved.

The invention provides a compound antioxidant for feed and a preparation method thereof. The technical scheme screens components having potential influence on oxygen free radicals based on experimental means, and researches interaction and synergistic relationship between each component and ethoxyquinoline. Experiments show that the corn ferment prepared by the specific process can generate a synergistic effect on ethoxyquin, and the synergistic effect is more remarkable when sodium alginate, magnesium lauryl sulfate, sodium carboxymethyl starch, ammonium glycyrrhizinate, acesulfame potassium, sucrose ester, sodium caseinate and arbutin are further introduced. Based on the beneficial findings, the invention constructs a compound antioxidant formula by using the components and provides a preparation method thereof. Experiments prove that the compound scheme of the invention can play a role in an extremely low dosage, can obviously improve the oxidation resistance of grease, and also improves the protection of fat-soluble vitamins to a certain extent, thereby having outstanding technical advantages.

Drawings

FIG. 1 is a graph showing the results of AOM-based oxidation resistance evaluation tests according to an embodiment of the present invention;

FIG. 2 is a graph showing the results of an oxidation resistance evaluation test based on the peroxide value of oils and fats according to the embodiment of the present invention;

FIG. 3 is a graph showing the results of an oxidation resistance evaluation test based on the acid value of oils and fats according to an embodiment of the present invention;

FIG. 4 is a graph showing the results of an antioxidant assessment test based on the protective effect of vitamin A in an embodiment of the present invention;

FIG. 5 is a graph showing the results of the antioxidant assessment test based on the vitamin E protective effect in the present embodiment.

Detailed Description

Hereinafter, specific embodiments of the present invention will be described in detail. Well-known structures or functions may not be described in detail in the following embodiments in order to avoid unnecessarily obscuring the details. Approximating language, as used herein in the following examples, may be applied to identify quantitative representations that could permissibly vary in number without resulting in a change in the basic function. Unless defined otherwise, technical and scientific terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

The corn ferments used in the examples were prepared by the following methods:

1) preparing an aqueous solution containing 0.27g/L of tea polyphenol, 0.74g/L of ethylene diamine tetraacetic acid and 0.96g/L of potassium citrate, soaking the corn cob cores in the aqueous solution, and continuously soaking for 48 hours at the temperature of 55 ℃; then discarding the liquid phase, crushing the wet rod core, centrifuging and taking the liquid phase to obtain a rod core leaching solution;

2) soaking corn stigma in BG-11 culture medium at normal temperature for 24h, draining, and pulverizing to obtain wet material; spreading the wet material on a tray to form a wet material layer with the thickness of 1-1.5 cm, and sprinkling trichoderma harzianum on the wet material layer according to the proportion of 20g of bacterial powder per kg of wet material; covering the upper end of the wet material layer with Trichoderma harzianum with corn flour in an amount which is 1.5 times the weight of the wet material; spraying lactobacillus delbrueckii on the corn powder layer according to the proportion of 15g of bacterial powder per kg of wet material; placing the tray at 33 ℃ under the condition of 1.2 atmospheric pressure, standing and fermenting for 72 h;

3) uniformly mixing all materials on a tray to obtain a fermented coarse material, mixing the rod core leaching solution obtained in the step 1) with the fermented coarse material with equal weight, adding water with the weight 6 times that of the fermented coarse material, uniformly mixing, and keeping for 1h under the ultrasonic oscillation condition at 47 ℃ to obtain a secondary fermentation substrate; inoculating waxy spore rod into the secondary fermentation substrateBacterium to 10⁶～10⁷CFU/mL, Rhodococcus erythropolis to 10⁶～10⁷CFU/mL, Pichia to 5X 10⁶～5×10⁷CFU/mL, and carrying out aerobic fermentation for 36h at the temperature of 36 ℃; and spreading the fermentation product, naturally air-drying, collecting the air-dried powder, and crushing the powder until the granularity is not less than 80 meshes to obtain the corn enzyme.

Example 1

Corn ferment.

Example 2

Ethoxyquinoline.

Example 3

A composite antioxidant for feed comprises the following components in parts by weight: 0.15 part of sodium alginate, 0.05 part of lauryl alcohol magnesium sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate and 0.04 part of arbutin.

Example 4

A composite antioxidant for feed comprises the following components in parts by weight: 0.15 part of sodium alginate, 0.05 part of lauryl alcohol magnesium sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate, 0.04 part of arbutin and 3.8 parts of corn ferment.

Example 5

A composite antioxidant for feed comprises the following components in parts by weight: 38.5 parts of ethoxyquinoline and 3.8 parts of corn ferment.

Example 6

A composite antioxidant for feed comprises the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.15 part of sodium alginate, 0.05 part of magnesium lauryl sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate and 0.04 part of arbutin.

Example 7

A composite antioxidant for feed comprises the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.15 part of sodium alginate, 0.05 part of magnesium lauryl sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate, 0.04 part of arbutin and 3.8 parts of corn ferment.

Example 8

A composite antioxidant for feed comprises the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.05 part of lauryl alcohol magnesium sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate, 0.04 part of arbutin and 3.8 parts of corn ferment.

Example 9

A composite antioxidant for feed comprises the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.15 part of sodium alginate, 0.05 part of lauryl alcohol magnesium sulfate, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate, 0.04 part of arbutin and 3.8 parts of corn ferment.

Example 10

A composite antioxidant for feed comprises the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.15 part of sodium alginate, 0.05 part of magnesium lauryl sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate and 3.8 parts of corn ferment.

1. Oxidation resistance evaluation test based on grease stability

AOM is a method of evaluating the oxidation resistance of fats and oils, expressed as the time during which the fat remains in a certain mass. The specific method comprises the following steps: taking a quantitative oil sample, heating at a constant temperature, and introducing quantitative air to accelerate the oxidation of the grease. The oxidation degree is checked by peroxide value (POV), the time from the air introduction to the peroxide value of 100 milliequivalents/kg is used as AOM value, the larger the AOM value is, the better the oxidation resistance of the grease is, different antioxidants are added into the same vegetable oil without the antioxidants, and the corresponding AOM value is determined, the larger the AOM value is, the better the oxidation resistance of the antioxidants is.

As shown in fig. 1, when ethoxyquin was not present, the addition of only zea mays ferment or other ingredients (examples 1, 3, and 4) did not exhibit oxidation resistance, and the oil stability was at the same level as that of the blank group. In the case of adding ethoxyquinoline, if no corn ferment is added and only other compounding ingredients are added (example 6), the oxidation resistance is equivalent to that of pure ethoxyquinoline (example 2), and no synergistic effect is shown. When ethoxyquinoline is formulated with corn ferment (examples 5, 8, 9, 10), the antioxidant properties are significantly enhanced. The synergistic effect is further enhanced when ethoxyquin, corn ferment and all other ingredients are present together (example 7).

2. Oxidation resistance evaluation test based on oil peroxide value and acid value

Taking 11 conical flasks with the same specification, adding 100g of soybean oil into each flask, taking 1 flask as a blank control sample, adding the antioxidant of examples 1-10 into the other l0 flask, and mixing for 5min under ultrasonic wave to make the mixture uniform. Sealing with preservative film, placing the conical flask in an oven at 50 deg.C, and measuring peroxide value and acid value after 10 days.

The determination of the peroxide number is carried out according to national standard CB5538-1995 and the results are expressed as millimoles of peroxide per kilogram of fat. The acid number was determined according to national standard CB/T5009.37-2003 and the results are expressed as milligrams per gram of oil (calculated as potassium hydroxide).

As shown in fig. 2 and 3, the relative performance of oxidation resistance when evaluated in terms of peroxide number and acid number is identical to that of experiment 1 above. The corn ferment has a synergistic effect on ethoxyquin, and when other components exist at the same time in a specific ratio, the synergistic effect is further enhanced. The experimental results are mutually verified.

3. Antioxidant evaluation test based on vitamin protection effect

Adding different antioxidants into the composite vitamins, carrying out a strengthening test, and measuring the content of VA and VE in a sample by using GB12388-90 high pressure liquid chromatography after 21 days to obtain the storage rate of VA and VE.

The experimental results are shown in fig. 4 and 5, ethoxyquinoline has good protection on VA and VE, although the effect can be enhanced to a certain extent by adding the compound component of the invention, the promotion is relatively limited, and particularly, the synergistic effect on the VE protection is not obvious.

4. Evaluation test of influence on feed performance

The AA broiler chickens of 1 day age are taken and randomly divided into 5 daily ration treatments, each treatment is 8 times repeated, each treatment is 100 times repeated, and the test period is 50 days.

Control group: the basal ration contained no antioxidant. After the test, the weight was measured, the amount of material consumed and the number of deaths were recorded daily, and the growth performance was determined. The test results are shown in table 1.

TABLE 1 feeding effect with different antioxidant feeds

As shown in table 1, although each experimental group has no obvious influence on the survival rate of experimental animals, the addition of corn enzyme and other components in the presence of ethoxyquin can significantly increase daily feed intake and weight gain rate, and increase feed conversion ratio to a certain extent, so that the composite antioxidant of the present invention has an effect of improving the overall feeding of the feed.

The embodiments of the present invention have been described in detail, but the description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention. Any modification, equivalent replacement, and improvement made within the scope of the application of the present invention should be included in the protection scope of the present invention.

Claims (8)

1. The composite antioxidant for the feed is characterized by comprising the following components in parts by weight: 35-42 parts of ethoxyquinoline, 0.12-0.18 part of sodium alginate, 0.03-0.07 part of magnesium lauryl sulfate, 0.01-0.09 part of sodium carboxymethyl starch, 0.03-0.05 part of ammonium glycyrrhizinate, 0.02-0.06 part of acesulfame potassium, 0.07-0.13 part of sucrose ester, 0.05-0.07 part of sodium caseinate, 0.02-0.06 part of arbutin and 3.2-4.4 parts of corn ferment.

2. The compound antioxidant for feed as claimed in claim 1, further comprising 0.06-0.08 part by weight of glutathione.

3. The compound antioxidant for feed as claimed in claim 1, further comprising 0.02-0.04 parts by weight of mannitol.

4. The compound antioxidant for feed as claimed in claim 1, further comprising 0.01-0.03 weight part of glyceryl monostearate.

5. The compound antioxidant for feed as claimed in claim 1, further comprising 0.08-0.12 weight part of xanthan gum.

6. The compound antioxidant for feed as claimed in claim 1, which is prepared from the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.15 part of sodium alginate, 0.05 part of magnesium lauryl sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate, 0.04 part of arbutin and 3.8 parts of corn ferment.

7. The compound antioxidant for feed as claimed in claim 1, which is prepared from the following components in parts by weight: 38.5 parts of ethoxyquinoline, 0.15 part of sodium alginate, 0.05 part of magnesium lauryl sulfate, 0.05 part of sodium carboxymethyl starch, 0.04 part of ammonium glycyrrhizinate, 0.04 part of acesulfame potassium, 0.1 part of sucrose ester, 0.06 part of sodium caseinate, 0.04 part of arbutin, 3.8 parts of corn ferment, 0.07 part of glutathione, 0.03 part of mannitol, 0.02 part of glycerol monostearate and 0.1 part of xanthan gum.

8. The method for preparing the compound antioxidant for feed as claimed in claim 1, which comprises the steps of:

1) preparing an aqueous solution containing 0.27g/L of tea polyphenol, 0.74g/L of ethylene diamine tetraacetic acid and 0.96g/L of potassium citrate, soaking the corn cob cores in the aqueous solution, and continuously soaking for 48 hours at the temperature of 55 ℃; then discarding the liquid phase, crushing the wet rod core, centrifuging and taking the liquid phase to obtain a rod core leaching solution;

2) soaking corn stigma in BG-11 culture medium at normal temperature for 24h, draining, and pulverizing to obtain wet material; spreading the wet material on a tray to form a wet material layer with the thickness of 1-1.5 cm, and sprinkling trichoderma harzianum on the wet material layer according to the proportion of 20g of bacterial powder per kg of wet material; covering the upper end of the wet material layer with Trichoderma harzianum with corn flour in an amount which is 1.5 times the weight of the wet material; spraying lactobacillus delbrueckii on the corn powder layer according to the proportion of 15g of bacterial powder per kg of wet material; placing the tray at 33 ℃ under the condition of 1.2 atmospheric pressure, standing and fermenting for 72 h;

3) uniformly mixing all materials on a tray to obtain a fermented coarse material, mixing the rod core leaching solution obtained in the step 1) with the fermented coarse material with equal weight, adding water with the weight 6 times that of the fermented coarse material, uniformly mixing, and keeping for 1h under the ultrasonic oscillation condition at 47 ℃ to obtain a secondary fermentation substrate; inoculating Bacillus cereus to the secondary fermentation substrate to 10⁶～10⁷CFU/mL, Rhodococcus erythropolis to 10⁶～10⁷CFU/mL, Pichia to 5X 10⁶～5×10⁷CFU/mL, and carrying out aerobic fermentation for 36h at the temperature of 36 ℃; spreading the fermentation product, naturally drying in air, collecting the dried powder, and crushing to obtain corn enzyme with the granularity not less than 80 meshes;

4) uniformly mixing 3.2-4.4 parts by weight of the corn ferment obtained in the step 3) with 35-42 parts by weight of ethoxyquinoline, 0.12-0.18 part by weight of sodium alginate, 0.03-0.07 part by weight of magnesium lauryl sulfate, 0.01-0.09 part by weight of sodium carboxymethyl starch, 0.03-0.05 part by weight of ammonium glycyrrhizinate, 0.02-0.06 part by weight of acesulfame potassium, 0.07-0.13 part by weight of sucrose ester, 0.05-0.07 part by weight of sodium caseinate and 0.02-0.06 part by weight of arbutin to obtain the composite antioxidant for the feed.

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