CN112361705A - Biological sample cryopreservation rack capable of achieving temperature control in partitioned mode and program temperature control equipment - Google Patents
Biological sample cryopreservation rack capable of achieving temperature control in partitioned mode and program temperature control equipment Download PDFInfo
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- CN112361705A CN112361705A CN202011144819.7A CN202011144819A CN112361705A CN 112361705 A CN112361705 A CN 112361705A CN 202011144819 A CN202011144819 A CN 202011144819A CN 112361705 A CN112361705 A CN 112361705A
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Classifications
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D31/00—Other cooling or freezing apparatus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D19/00—Arrangement or mounting of refrigeration units with respect to devices or objects to be refrigerated, e.g. infrared detectors
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D25/00—Charging, supporting, and discharging the articles to be cooled
- F25D25/02—Charging, supporting, and discharging the articles to be cooled by shelves
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D29/00—Arrangement or mounting of control or safety devices
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- Engineering & Computer Science (AREA)
- Thermal Sciences (AREA)
- Physics & Mathematics (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Combustion & Propulsion (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
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Abstract
The invention provides a biological sample cryopreservation rack capable of controlling temperature in a partitioned manner and program temperature control equipment, which are characterized in that: the device comprises a heat sink cold plate, a cooling unit and a control module, wherein the upper surface of the heat sink cold plate is connected with a plurality of independent cooling units, the control module is connected with and controls the cooling units, and the lower surface of the heat sink cold plate is connected with a refrigeration source; the cooling unit comprises a sample groove, a heating element, a temperature sensor and a heat insulation cover. The invention has the beneficial effects that: through designing independent cooling unit and heat sink, this equipment can be to the sample multichannel independent operation in batches of gathering at different times, accepts independent cooling unit's temperature sensor's data through control module, adjusts heating element's operating power, makes the freezing sample lower the temperature with best cooling rate, reaches best freezing effect.
Description
Technical Field
The invention relates to the field of biological low-temperature storage, in particular to a biological sample cryopreservation rack capable of controlling temperature in a partitioned mode and program temperature control equipment.
Background
In the preservation of cells or biological samples, freezing the sample to ultra-low temperatures (below-80 degrees celsius) and maintaining it at that temperature for an extended period of time using suitable methods is a critical element of the biological freezing technique. The usual method of preservation is to suspend the sample in a solution with cryoprotectant and store it for a long period after cooling to ultra-low temperature at a certain freezing rate. The method for precisely controlling the cooling rate is to adopt a program cooling instrument and reduce the temperature of a specific biological sample according to a preset curve through software.
The method generally has the defects of large liquid nitrogen consumption, large temperature control error, complex safety operation, potential risk of polluting biological samples when liquid nitrogen contacts the samples, heavy instrument structure, difficulty in transportation and the like. More importantly, the single operation is long in time, the consumption of liquid nitrogen is large, and a certain number of biological samples are collected and operated in batches when the single operation is used. In the use environment of hospitals or scientific laboratories, a small amount of precious samples, such as various experimental cell samples, oocytes, patient stem cells and the like, need to be frozen at a controlled rate as soon as possible while being collected, so that higher activity can be obtained during thawing, and the effectiveness of the next experiment/treatment can be guaranteed. Therefore, in order to meet the requirement of the fast-developing biomedicine on the cryopreservation of the biological samples, accurately control the temperature reduction process of the samples and realize the random access at the same time of storage, the invention provides the biological sample cryopreservation rack capable of controlling the temperature in a partitioned manner and the program temperature reduction equipment.
Disclosure of Invention
In order to solve the technical problems, the invention discloses a biological sample cryopreservation rack capable of controlling temperature in a partitioned mode and program temperature control equipment, and the technical scheme is implemented as follows:
a biological sample cryopreservation rack capable of controlling temperature in a partitioned mode comprises a heat sink cold plate, a cooling unit and a control module; the upper surface of the heat sink cold plate is connected with a plurality of independent cooling units, the control module is connected with and controls the cooling units, and the cooling units comprise sample tanks, heating elements, temperature sensors and heat insulation covers; the heating element and the temperature sensor are positioned on the side wall of the sample groove, and the heat insulation cover is positioned on the upper part of the sample groove.
Preferably, the heat sink cold plate includes the benchmark hole, heat sink cold plate upper surface is provided with concave-convex structure, the cooling unit includes that the sample holds in the palm and the spliced pole, the spliced pole is located the sample holds in the palm the bottom, the spliced pole with the benchmark hole matches fixedly, the inside multicircuit passageway that is provided with of heat sink cold plate, heat sink cold plate upper surface covers there is heat-insulating material.
Preferably, the size of the sample groove aperture is 1-100mm, the depth of the sample groove is 1-200mm, and the number of the sample holes in the sample groove is 1 or more.
Preferably, the cooling device further comprises a thermal connector, and the cooling unit is fixedly connected with the heat sink cold plate through the thermal connector.
Preferably, the thermal connecting piece is made of sapphire, and the thickness of the thermal connecting piece is 0.1-20 mm.
Preferably, the thermal connection comprises a micro-expansion type low temperature thermal switch.
A programmed cooling device comprising a temperature-divisionally controllable biological specimen cryopreservation rack according to any one of claims 1 to 6, a specimen cryopreservation rack housing and a refrigeration unit; the refrigeration unit comprises a refrigeration source, the refrigeration source is located in the refrigeration unit, and the refrigeration source is in contact with the lower surface of the heat sink cold plate.
Preferably, the refrigeration source is a refrigerator; the refrigerator comprises a cold head, and the cold head is connected with the lower surface of the heat sink cold plate.
Preferably, the refrigeration source is a refrigerant; the refrigeration unit comprises a Dewar vessel, the refrigerant is loaded in the Dewar vessel, and an opening of the Dewar vessel is coupled with the lower surface of the heat sink cold plate.
By implementing the technical scheme of the invention, the technical problems that in the prior art, the liquid nitrogen consumption is large, the temperature control error is large, the safety operation is complicated, the liquid nitrogen is in contact with a sample, the risk of potentially polluting a biological sample exists, and an instrument structure is heavy and cannot be easily transported can be solved; by implementing the technical scheme of the invention, the plurality of independent cooling areas are arranged on the sample freezing rack, so that a plurality of groups of samples can be operated in batches on the premise of not moving out the samples, the sample freezing rack can be butted with various refrigeration equipment to realize a program cooling function, and the technical effect of short-term low-temperature storage function of the biological samples is achieved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only one embodiment of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
In which like parts are designated by like reference numerals. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "bottom" and "top," "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.
FIG. 1 is a schematic structure diagram of a biological sample freezing rack capable of controlling temperature in a partitioned manner;
FIG. 2 is a schematic structural diagram of a biological sample cryopreservation rack capable of controlling temperature in a partitioned manner;
FIG. 3 is a schematic structural view of example 2;
FIG. 4 is a schematic view of a connection structure of a thermal connector and a heat sink cold plate;
fig. 5 is a schematic structural view of embodiment 4.
In the above drawings, the reference numerals denote:
1, heat sink cold plate
1-1, reference hole
2, a cooling unit
2-1, sample cell
2-2, heating element
2-3, temperature sensor
2-4, heat insulation cover
2-5, sample holder
2-6, connecting column
3, control module
4, thermal connection
5, sample freezing rack shell
6, a cooling unit
6-1, cold head
6-2 Dewar vessel
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
In a specific embodiment 1, as shown in fig. 1 and fig. 2, a biological sample cryopreservation rack capable of controlling temperature in a partitioned manner comprises a heat sink cold plate 1, a temperature reduction unit 2 and a control module 3; the upper surface of the heat sink cold plate 1 is connected with a plurality of independent cooling units 2, the control module 3 is connected with and controls the cooling units 2, and each cooling unit 2 comprises a sample tank 2-1, a heating element 2-2, a temperature sensor 2-3 and a heat insulation cover 2-4; the heating element 2-2 and the temperature sensor 2-3 are positioned on the side wall of the sample groove 2-1, and the heat insulation cover 2-4 is positioned on the upper part of the sample groove 2-1.
In this embodiment, the heat sink cold plate 1 is made of a metal material with high thermal conductivity, such as aluminum, copper, alloy, etc., the upper end of the heat sink cold plate 1 is provided with a plurality of independent cooling units 2, and each cooling unit 2 includes a sample tank 2-1, a heating element 2-2, a temperature sensor 2-3, and a heat insulation cover 2-4. The single sample groove 2-1 is made of metal materials with high heat conductivity such as aluminum, copper, alloy and the like and can be used for loading a sample tube, and the heat insulation cover 2-4 on the upper part of the sample groove 2-1 can cover the exposed part of the sample tube to isolate the influence of the change of the heat energy of the surrounding environment on the sample tube. The heating element 2-2 and the temperature sensor 2-3 are fixed on the side wall of the single sample groove 2-1, the temperature sensor 2-3 collects the temperature of the sample groove 2-1 (namely representing the current sample temperature) and feeds the temperature back to the control module 3, and the control module 3 controls the power of the heating element 2-2 according to the fed temperature. The control module 3 can be a singlechip, a POC (programmable logic controller), a PC (personal computer) system and the like with a processor. The temperature sensor 2-3 can be various thermocouple thermometers and thermal resistance thermometers, the heating element 2-2 can be various heaters, such as a ceramic heater, a semiconductor heater, a film heater and the like, the power of the heating element 2-2 can be 1W to 200W, when the temperature sensor is used, the lower surface of the heat sink cold plate 1 can be directly contacted with a refrigeration source, and the refrigeration source can be liquid nitrogen, liquid helium, liquid hydrogen, liquid fluorine, liquid oxygen, liquid methane, various Stirling refrigerators, GM refrigerators, J-T refrigerators, pulse tube refrigerators and the like. Because the cold volume that the refrigeration source provided is sufficient, the temperature of heat sink cold drawing 1 is not influenced by 2 heat energy size changes of upper end cooling unit, and the heating element 2-2 and the temperature sensor 2-3 of a plurality of cooling unit 2 are independent each other, consequently can be independent carry out the procedure cooling to single cooling unit 2, the cryopreservation, quick rewarming etc. operation, realize 2 time sharing subregion cooling of a plurality of cooling units, storage, rewarming, and to the biological sample of different specialties, can cool down with the best speed of difference simultaneously, reach best cryopreservation effect.
Taking processing two samples a and B to be frozen as an example, in practical use, after the heat sink-cold plate 1 is butted with a refrigeration source such as a refrigerant or a refrigerator, the temperature of the heat sink-cold plate 1 is rapidly reduced to an ultra-low temperature, meanwhile, the heat sink-cold plate 1 is continuously in a low-temperature state, and the temperature of the temperature reduction units 2A and 2B of the heating elements 2-2A and 2-2B which are not started is also reduced to the ultra-low temperature. Since the initial temperature of the samples A and B to be frozen is typically near room or refrigerated temperature, the heating elements 2-2A, 2-2B are activated to maintain the initial temperature of the sample wells 2-1A, 2-1B at 4 ℃ to 20 ℃ before the samples are placed in the sample wells 2-1A, 2-1B for controlled cooling. After the temperatures of the sample tanks 2-1A and 2-1B reach the initial set values, the sample A can be put in, the temperature reduction rate vA (v, t) of the sample tank 2-1A is preset through the control module 3, and a program temperature reduction process is started. The sample B can be placed at any time, the temperature reduction rate vB (v, t) of the sample tank 2-1B is preset through the control module 3, and the program temperature reduction process is started. In the process of programmed cooling, the temperature sensors 2-3A, B respectively read the current temperatures of the sample tanks 2-1A, B and feed the temperatures back to the control module 3, and the control module 3 adjusts the power of the heating elements 2-2A and 2-2B according to the temperatures, so that the temperatures of the sample tanks 2-1A and 2-1B respectively change according to a preset cooling curve, and the purpose of accurate cooling is achieved. When the temperature of the sample tanks 2-1A, 2-1B is reduced to the set value, the control module 3 will maintain the heating elements 2-2A, 2-2B at the current power value, and continuously monitor the temperature of the sample tanks 2-1A, 2-1B to keep it stable. If necessary, the control module 3 can also control the heating elements 2-2A and 2-2B to heat the sample tanks 2-1A and 2-1B in the low-temperature state, so that the frozen samples can be rapidly rewarming.
The embodiment has a plurality of independent cooling areas, can operate a plurality of groups of samples in batches on the premise of not moving out the samples, realizes random access along with storage, shortens the waiting time from the collection of the samples to the freezing, improves the recovery effect of the samples, and can also simultaneously cool a plurality of samples at different optimal cooling rates to achieve the optimal freezing and storing effect. The program cooling device adopting the refrigerating machine as a refrigerating source is small in structure, and the mobile power supply is configured to be suitable for use scenes such as long-distance transportation of biological samples, field and multi-place biological sample collection and the like. The method adopts the refrigerant as refrigeration source program cooling equipment, has low refrigerant consumption and high refrigeration efficiency, can realize batch operation of a large number of samples, and is suitable for large-scale GMP cell production centers and the storage requirements of biological sample libraries on a large number of biological samples.
Example 2
In a preferred embodiment 2, as shown in fig. 1, 2 and 3, the heat sink-cold plate 1 includes a reference hole 1-1, the upper surface of the heat sink-cold plate 1 is provided with a concave-convex structure, the cooling unit 2 includes a sample holder 2-5 and a connecting column 2-6, the connecting column 2-6 is located at the bottom of the sample holder 2-5, the connecting column 2-6 is matched and fixed with the reference hole 1-1, a multi-circuit channel is arranged inside the heat sink-cold plate 1, and the upper surface of the heat sink-cold plate 1 is covered with a heat insulating material.
In this embodiment, the upper surface of the heat sink cold plate 1 is designed to be a concave-convex structure, the cooling unit 2 is fixed on the concave-convex structure corresponding to the heat sink cold plate 1 in a plugging manner through the sample holder 2-5, the sample holder 2-5 can adopt a multi-pin plugging type sample holder commonly used for sample measurement at low temperature, the bottom of the sample holder 2-5 is provided with a connecting column 2-6, such as 10pin or 12pin, etc., a reference hole 1-1 is arranged in the protruding part of the heat sink cold plate 1, and the aperture can just accommodate the connecting column 2-6. The cooling unit 2 is fixed on the sample support 2-5, the heating element 2-2 and the control circuit of the temperature sensor 2-3 in the cooling unit 2 are connected with the datum hole 1-1 on the heat sink cold plate 1 through the connecting column 2-6 of the sample support 2-5, and then the multichannel circuit inside the heat sink cold plate 1 is connected to the control module 3, so that the wires are prevented from being exposed outside too much, the cooling unit 2 is convenient to insert, pull out, maintain and replace, and the main body of the sample support 2-5 can be made of metal materials with good heat conductivity. Meanwhile, the surface of the heat sink cold plate 1 is covered with a heat insulating material, so that the groove gap and the gaps of the plurality of cooling units 2 are filled with the heat insulating material, the heat insulating material and the heat insulating covers 2-4 are used for avoiding the influence of the heat radiation of the surrounding environment on the cooling units 2, and meanwhile, the single independent cooling unit 2 is only in contact heat conduction with the heat sink cold plate 1.
In a preferred embodiment, the sample well 2-1 has a pore size of 1 to 100mm, the sample well 2-1 has a depth of 1 to 200mm, and the number of sample wells in the sample well 2-1 is 1 or more.
Because the sample groove 2-1 is used for loading sample tubes, in the actual biological low-temperature preservation, the sample tubes have different specifications, such as freezing tubes, straws, vaccines, medicines, blood bags and the like, the pore size and the depth inside the sample groove 2-1 also need to be matched with the sample tubes with different specifications, the depth of the sample groove 2-1 needs to expose part of tube bodies and tube caps of the sample tubes, so that the storage and the taking operation are convenient, a single sample groove 2-1 can contain at least one frozen sample, and can also be designed to be matched with specifications of various porous plates and SBS standard porous plates to contain a plurality of samples, so that the sample can be conveniently combined with a cell production line for use. When the sample tube to be processed is a straw, the upper surface of the sample groove 2-1 is designed into a transverse groove to accommodate the straw in a slender shape, and when the sample to be processed is a blood bag, the upper surface of the sample groove 2-1 is designed into a plane.
Example 3
In a preferred embodiment 3, as shown in fig. 1, fig. 2 and fig. 4, a thermal connector 4 is further included, and the cooling unit 2 is connected and fixed with the heat sink cold plate 1 through the thermal connector 4.
In the case where the refrigeration capacity provided by the refrigerant and the refrigerator is very large and the temperature of the heat sink cold plate 1 is very low (e.g., less than-120 ℃), if the sample tank 2-1 is in direct contact with the heat sink cold plate 1, the power of the heating element 2-2 may not be sufficient to maintain the initial temperature of the sample tank stable within the temperature range of 4 ℃ to 20 ℃. Therefore, by adopting the thermal connector 4, when the heating element 2-2 is started to increase the temperature of the sample cell 2-1, the thermal connector 4 is influenced by the temperature change of the sample cell 2-1, the temperature of the thermal connector 4 is increased while the thermal conductivity is reduced, the heat transmission from the sample cell 2-1 to the heat sink cold plate 1 is reduced, the further temperature increase of the sample cell 2-1 is promoted, and the heating element 2-2 can work at a lower power to maintain the initial temperature of the sample cell to be stabilized in a temperature range from 4 ℃ to 20 ℃. After the cooling program is started, the heat conductivity of the thermal connecting piece 4 is increased along with the reduction of the temperature of the sample groove 2-1, so that the heat is promoted to be transmitted from the sample groove 2-1 to the heat sink cold plate 1, the adjustable cooling speed range is wider, and the freezing requirement of more types of biological samples can be met.
In a preferred embodiment, the thermal connector 4 is made of sapphire, and the thickness of the thermal connector 4 is 0.1-20 mm.
Since the thermal conductivity increases with decreasing temperature and decreases with increasing temperature in a temperature range of 30K or more, such as sapphire, weak thermal conductivity at high temperature and good thermal conductivity at low temperature can be exhibited. The requirements of controlling the temperature rise and the temperature drop of the sample tank 2-1 by using the heating element 2-2 can be effectively met.
In a preferred embodiment, the thermal connection 4 comprises a micro-expansion type low temperature thermal switch.
In the embodiment, the thermal connector 4 directly adopts a micro-expansion type low-temperature thermal switch, and the effects of opening the thermal connector 4 at high temperature and closing and conducting heat at low temperature are realized by utilizing the different expansion and contraction rates of two different materials, and the requirements of controlling the temperature rise and the temperature drop of the sample tank 2-1 by utilizing the heating element 2-2 can also be met.
Example 4
In a specific embodiment 4, a programmable cooling device, as shown in fig. 1, 2 and 5, comprising a zonally temperature-controllable biological specimen cryopreserving rack according to any one of claims 1-7, a specimen cryopreserving rack housing 5 and a refrigeration unit 6; the refrigerating unit 6 comprises a refrigerating source, the refrigerating source is positioned in the refrigerating unit 6, and the refrigerating source is in contact with the lower surface of the heat sink cold plate 1.
In this embodiment, a programmed cooling device using the temperature-divisionally controllable biological specimen cryopreservation rack of any one of claims 1 to 7 is disclosed, in order to isolate the influence of external environmental heat, the housing 5 of the specimen cryopreservation rack may be designed as a double-layer vacuum dewar or as a heat insulation device such as a vacuum insulation panel and a polyurethane foam composite material, and the refrigeration source of the refrigeration unit 6 may be various types of refrigeration equipment or refrigerants such as liquid nitrogen, liquid helium, liquid hydrogen, liquid fluorine, liquid oxygen, liquid methane, and various types of stirling refrigerators, GM refrigerators, J-T refrigerators, pulse tube refrigerators, and the like.
In a preferred embodiment, the refrigeration source is a refrigerator; the refrigerator comprises a cold head 6-1, and the cold head 6-1 is connected with the lower surface of the heat sink cold plate 1.
When the refrigerating source adopts a refrigerator, the cold head 6-1 of the refrigerator is in direct contact with the lower surface of the heat sink cold plate 1, and the heat of the sample cryopreservation rack is removed in a contact heat conduction mode. And a damping device is arranged between the refrigerating machine and the sample freezing rack so as to reduce potential damage to the biological sample caused by vibration. The program cooling equipment adopting a single refrigerator is small and exquisite in structure, and a mobile power supply is configured to be suitable for the use scenes of long-distance transportation of biological samples, field and multi-site biological sample collection and random access.
In a preferred embodiment, the refrigeration source is a refrigerant; the refrigeration unit 6 comprises a Dewar vessel 6-2, refrigerant is loaded in the Dewar vessel 6-2, and an opening of the Dewar vessel 6-2 is coupled with the lower surface of the heat sink cold plate 1.
When various types of refrigerants are adopted as the refrigeration source of the refrigeration unit 6, the refrigerants can be loaded in a Dewar vessel 6-2, the opening of the Dewar vessel 6-2 is coupled with the heat sink cold plate 1, and the heat of the sample freezing rack is removed in a contact heat conduction mode.
In the present invention:
the sample freezing frame can independently cool samples collected at different times in batches and in multiple channels, shortens the waiting time from the collection to the freezing of the samples, improves the recovery effect of the samples, and can cool a plurality of samples at different optimal cooling rates simultaneously to achieve the optimal freezing and storing effect.
The sample freezing rack coupled with the refrigerant refrigerating source can realize the procedure cooling process with lower refrigerant consumption.
The sample freezing frame is coupled with the refrigerating source of the refrigerating machine, so that liquid nitrogen-free refrigeration can be realized, the biological sample is prevented from being polluted by liquid nitrogen, and the operation is safe and no material consumption is caused.
Each cooling unit 2 of sample cryopreserving frame is independent each other, and the sample that has accomplished the cooling can not need to take out in the short time, does not receive the influence of other sample cooling operations, can possess short-term low temperature and store the function.
The temperature of each cooling unit 2 is adjusted by a heating element 2-2 and a temperature sensor 2-3, and the sample can be controlled to be rapidly reheated for use besides temperature reduction.
The temperature can be regulated and controlled to be +/-0.1 ℃.
It should be understood that the above-described embodiments are merely exemplary of the present invention, and are not intended to limit the present invention, and that any modification, equivalent replacement, or improvement made without departing from the spirit and principle of the present invention shall fall within the protection scope of the present invention.
Claims (9)
1. The utility model provides a but biological sample cryopreserving frame of subregion accuse temperature which characterized in that: the device comprises a heat sink cold plate, a cooling unit and a control module; the upper surface of the heat sink cold plate is connected with a plurality of independent cooling units, the control module is connected with and controls the cooling units, and the cooling units comprise sample tanks, heating elements, temperature sensors and heat insulation covers; the heating element and the temperature sensor are positioned on the side wall of the sample groove, and the heat insulation cover is positioned on the upper part of the sample groove.
2. The cryopreservation rack for biological samples capable of controlling temperature according to claim 1, wherein: the heat sink cold plate comprises a reference hole, the upper surface of the heat sink cold plate is provided with a concave-convex structure, the cooling unit comprises a sample support and a connecting column, the connecting column is located at the bottom of the sample support, the connecting column is matched and fixed with the reference hole, a multi-circuit channel is arranged inside the heat sink cold plate, and the upper surface of the heat sink cold plate is covered with a heat-insulating material.
3. The cryopreservation rack for biological samples capable of controlling temperature according to claim 1, wherein: the aperture of the sample groove is 1-100mm, the depth of the sample groove is 1-200mm, and the number of sample holes in the sample groove is 1 or more than one.
4. The cryopreservation rack for biological samples capable of controlling temperature according to claim 1, wherein: the cooling unit is fixedly connected with the heat sink cold plate through the thermal connecting piece.
5. The cryopreservation rack for biological samples capable of being controlled by temperature according to claim 4, wherein: the thermal connecting piece is made of sapphire, and the thickness of the thermal connecting piece is 0.1-20 mm.
6. The cryopreservation rack for biological samples capable of being controlled by temperature according to claim 4, wherein: the thermal connection comprises a micro-expansion type low-temperature thermal switch.
7. A program cooling device, characterized in that: comprising a temperature-divisionally controllable biological specimen cryopreservation rack according to any one of claims 1 to 6, a specimen cryopreservation rack housing and a refrigeration unit; the refrigeration unit comprises a refrigeration source, the refrigeration source is located in the refrigeration unit, and the refrigeration source is in contact with the lower surface of the heat sink cold plate.
8. A program cooling device according to claim 7, wherein: the refrigeration source is a refrigerator; the refrigerator comprises a cold head, and the cold head is connected with the lower surface of the heat sink cold plate.
9. A program cooling device according to claim 7, wherein: the refrigeration source is a refrigerant; the refrigeration unit comprises a Dewar vessel, the refrigerant is loaded in the Dewar vessel, and an opening of the Dewar vessel is coupled with the lower surface of the heat sink cold plate.
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Cited By (3)
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| CN113686071A (en) * | 2021-07-05 | 2021-11-23 | 李苗 | Cold chain interruption color development early warning type vaccine refrigeration device |
| CN114323568A (en) * | 2022-03-14 | 2022-04-12 | 武汉普赛斯电子技术有限公司 | Three-temperature testing system of optical device |
| CN117739589A (en) * | 2024-02-21 | 2024-03-22 | 季华实验室 | Multi-cavity dewar tank based on fixed cold source and temperature regulating system |
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- 2020-10-23 CN CN202011144819.7A patent/CN112361705A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113686071A (en) * | 2021-07-05 | 2021-11-23 | 李苗 | Cold chain interruption color development early warning type vaccine refrigeration device |
| CN114323568A (en) * | 2022-03-14 | 2022-04-12 | 武汉普赛斯电子技术有限公司 | Three-temperature testing system of optical device |
| CN117739589A (en) * | 2024-02-21 | 2024-03-22 | 季华实验室 | Multi-cavity dewar tank based on fixed cold source and temperature regulating system |
| CN117739589B (en) * | 2024-02-21 | 2024-05-14 | 季华实验室 | Multi-cavity dewar tank based on fixed cold source and temperature regulating system |
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