CN112358949A

CN112358949A - A sterilization fermentation cylinder for preparing cell culture base

Info

Publication number: CN112358949A
Application number: CN202011352434.XA
Authority: CN
Inventors: 王兆晶
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-27
Filing date: 2020-11-27
Publication date: 2021-02-12

Abstract

The invention provides a sterilization fermentation tank for preparing a cell culture medium, which structurally comprises a support, a controller, a feeding port, a partition plate and a fermentation tank, wherein the support is fixed at the lower end of the fermentation tank; according to the invention, the cell reagent and the steam in the fermentation tank can be stirred by the stirring mechanism arranged in the fermentation tank, so that the scraper blade passing through the sliding block can drive the driving device, and the scraper blade and the spring can continuously extend and contract under the action of the spring, so that the scraper blade can effectively push the steam in the fermentation tank, thereby increasing the contact area of the steam and the cell reagent to realize the rapid sterilization effect by mixing.

Description

A sterilization fermentation cylinder for preparing cell culture base

Technical Field

The invention relates to the field of sterilization of culture media, in particular to a sterilization fermentation tank for preparing a cell culture medium.

Background

The cell culture is that the prepared cells and reagents are gradually put into a fermentation tank in batches, so that the cells can be mixed with the corresponding reagents in the fermentation tank, meanwhile, the cell reagents can be sterilized by steam emitted by heating the inside of the fermentation tank, the cell reagents can form a sterile state after being sterilized by the steam, and then the cell reagents are fermented in a closed space of the fermentation tank;

in conclusion, the inventor finds that the existing sterilization fermentation tank mainly has the following defects: because the steam generated by the fermentation tank during heating can continuously float at the upper end of the cell reagent, and the fermentation tank cannot increase the circulation speed of the steam, the steam expands the contact area and is merged into the cell reagent, the sterilization time of the steam on the cell reagent is increased, and the cell reagent can form uneven sterilization, so that bacteria in the cell reagent cannot be completely killed.

Disclosure of Invention

The technical scheme adopted by the invention for realizing the technical purpose is as follows: the utility model provides a sterilization fermentation cylinder for preparing cell culture base, its structure includes support, controller, pan feeding mouth, partition panel, fermentation cylinder, the support is fixed in the lower extreme of fermentation cylinder, the controller is installed in the outer lane position of fermentation cylinder, pan feeding mouth and partition panel are located the upper end of partition panel and are in the state of communicating with each other, the fermentation cylinder upper end is connected and communicates with each other with the partition panel.

Inside sealing washer, rabbling mechanism, activity space, the bucket of breathing in of being equipped with of fermentation cylinder, the rabbling mechanism has been inlayed to the sealing washer middle part, the activity space is located the outer lane position of rabbling mechanism, the bucket of breathing in is installed on the inner circle edge of sealing washer, the bucket of breathing in is equipped with six altogether to evenly distributed is in each extreme point of sealing washer.

As a further improvement of the invention, the stirring mechanism is provided with a driving device, a sliding block, springs, scraping blades and a connecting frame, wherein the driving device and the sliding block form an opposite surface state, the springs are distributed on the outer ring part of the sliding block, the scraping blades are welded at the upper ends of the springs and are movably matched, the connecting frame is fixed on two sides of the scraping blades, and the connecting frame connects each scraping blade with the sliding block together.

As a further improvement of the invention, the driving device is provided with a protective cover, a stress block, a large rotating wheel, a sliding belt and a fixed seat, the protective cover is embedded in the edge position of the stress block, the large rotating wheel is arranged at the lower end of the protective cover, the sliding belt is embedded in the middle of the protective cover and is movably connected with the large rotating wheel, the fixed seat is connected with the bottom of the sliding belt and is movably matched with the large rotating wheel, and the large rotating wheel can drive the part of the fixed seat through the sliding belt, so that the stress block can be extended.

As a further improvement of the invention, the fixed seat is provided with a sensor, a connecting wire, a small rotating wheel, a threaded rod and a connector, the sensor is arranged below the small rotating wheel and is connected with the small rotating wheel through the connecting wire, the threaded rod is embedded in the inner side of the small rotating wheel and is movably matched with the inner side of the small rotating wheel, the connector and the threaded rod are integrated, and two ends of the sensor are respectively connected with the threaded rod and the small rotating wheel.

As a further improvement of the invention, the air suction barrel is provided with an adsorption shaft, an extractor, an arc-shaped plate and a guide pipe, the adsorption shaft is arranged above the extractor, the arc-shaped plate and the adsorption shaft are integrated and are positioned below the adsorption shaft, the guide pipe penetrates through the arc-shaped plate and is connected with the extractor, and the middle part of the arc-shaped plate penetrates through the guide pipe.

As a further improvement of the invention, the catheter is provided with a solid layer, an extension port, a rotary groove, a push block and an exhaust plate, the solid layer and the extension port are positioned at the same central point, the rotary groove is mounted on the outer ring of the extension port and rotates, the push block is distributed on the outer ring of the rotary groove, the exhaust plate is welded at the upper end of the push block, and the exhaust plate can prevent cell reagents from being poured into the extension port through the push block and the rotary groove.

Compared with the prior art, the invention has the following beneficial effects:

1. according to the invention, the cell reagent and the steam in the fermentation tank can be stirred by the stirring mechanism arranged in the fermentation tank, so that the scraper blade passing through the sliding block can drive the driving device, and the scraper blade and the spring can continuously extend and contract under the action of the spring, so that the scraper blade can effectively push the steam in the fermentation tank, thereby increasing the contact area of the steam and the cell reagent to realize the rapid sterilization effect by mixing.

2. The invention can extract a part of steam emitted by the fermentation tank under the action of the air suction barrel, so that the part of steam can be transmitted to the bottommost part of the cell reagent, the bottommost part of the cell reagent can be sterilized by the steam at the first time, and the cells can be prevented from being directly poured into the guide pipe by the mutual continuous movement of the solid layer, the rotary groove and the pushing block, so that the exhaust plate can effectively exhaust the steam, and the sterilization effects of the upper side and the lower side are balanced.

Drawings

FIG. 1 is a schematic diagram of a sterilized fermenter for preparing a cell culture medium.

FIG. 2 is a schematic view of a top view of the interior of a fermenter.

Fig. 3 is a schematic top view of a stirring mechanism.

Fig. 4 is a schematic top view of a driving device.

Fig. 5 is a schematic top view of a fixing base.

Fig. 6 is a schematic top view of a suction barrel.

Fig. 7 is a front view of a catheter with components.

In the figure: support-1, controller-2, material inlet-3, partition board-4, fermentation tank-5, sealing ring-51, stirring mechanism-52, movable space-53, air suction barrel-54, driving device-521, slide block-522, spring-523, scraping blade-524, connecting frame-525, protective cover-a 1, stress block-a 2 and large rotating wheel-a 3, the device comprises a slide belt-a 4, a fixed seat-a 5, a sensor-a 51, a connecting line-a 52, a small rotating wheel-a 53, a threaded rod-a 54, a connecting head-a 55, an adsorption shaft-541, an extractor-542, an arc plate-543, a guide pipe-545, a solid layer-b 11, an extension port-b 12, a rotating groove-b 13, a push block-b 14 and an exhaust plate-b 15.

Detailed Description

The invention is further described below with reference to the accompanying drawings:

example 1:

fig. 1 to 5 show:

the invention provides a sterilization fermentation tank for preparing a cell culture medium,

its structure includes support 1, controller 2, pan feeding mouth 3, partition panel 4, fermentation cylinder 5, support 1 is fixed in the lower extreme of fermentation cylinder 5, controller 2 installs in the outer lane position of fermentation cylinder 5, pan feeding mouth 3 is located the upper end of partition panel 4 and is in the state that communicates with each other with partition panel 4, fermentation cylinder 5 upper end is connected and communicates with each other with partition panel 4.

The fermentation tank 5 is internally provided with a sealing ring 51, a stirring mechanism 52, a movable space 53 and an air suction barrel 54, the stirring mechanism 52 is embedded in the middle of the sealing ring 51, the movable space 53 is positioned at the outer ring part of the stirring mechanism 52, the air suction barrel 54 is arranged on the inner ring edge of the sealing ring 51, the number of the air suction barrel 54 is six, and the air suction barrel 54 is evenly distributed at each end point of the sealing ring 51, so that the air suction barrel 54 can effectively absorb steam and then discharge the steam into the bottom of the cell reagent.

The stirring mechanism 52 is provided with a driving device 521, a sliding block 522, a spring 523, a scraping blade 524 and a connecting frame 525, the driving device 521 and the sliding block 522 form an opposite surface state, the spring 523 is distributed on the outer ring part of the sliding block 522, the scraping blade 524 is welded on the upper end of the spring 523 and is movably matched with the spring, the connecting frame 525 is fixed on two sides of the scraping blade 524, the connecting frame 525 connects the scraping blades 524 with the sliding block 522 together, so that the connecting frame 525 effectively fixes the scraping blade 524 and the sliding block 522, and the positions of the scraping blade 524 and the sliding block 522 can be kept consistent under the action of rotation.

The driving device 521 is provided with a protective cover a1, a stress block a2, a large runner a3, a sliding belt a4 and a fixed seat a5, the protective cover a1 is embedded in the edge position of the stress block a2, the large runner a3 is installed at the lower end of the protective cover a1, the sliding belt a4 is embedded in the middle of the protective cover a1 and movably connected with the large runner a3, the fixed seat a5 is connected with the bottom of the sliding belt a4 and movably matched with the large runner a3, the large runner a3 can drive the part of the fixed seat a5 through the sliding belt a4, so that the stress block a2 can be extended, and the large runner a3 can be driven by other parts, so that the large runner a4 can drive the fixed seat a5 to process the stress block a2 for extrusion and extension.

The fixed seat a5 is provided with a sensor a51, a connecting line a52, a small runner a53, a threaded rod a54 and a connector a55, the sensor a51 is installed below the small runner a53 and connected with the small runner a53 through the connecting line a52, the threaded rod a54 is embedded into the inner side of the small runner a53 and is movably matched with the inner side of the small runner a53, the connector a55 and the threaded rod a54 are integrated, two ends of the sensor a51 are respectively connected with the threaded rod a54 and the small runner a53, the sensor a51 can enable the connector a55 to extrude other components to the bottom and then transmit signals to enable the small runner a53 to rotate rapidly, and all the components can return to the original positions.

The specific functions and operation flow of this embodiment are as follows:

in the invention, cell reagent is put into the fermentation tank 5 through the feeding port 3, so that the fermentation tank 5 is opened through the controller 2, the fermentation tank 5 can start to heat to generate steam, bacteria in the cell reagent can be killed by the steam, meanwhile, the stirring mechanism 52 operates through the movable space 53, the sliding block 522 can drive the scraping blade 524 when rotating, the spring 523 can extend when rotating, the contact area of the scraping blade 524 can be enlarged, when the scraping blade 524 is enlarged to the driving device 521, the large rotating wheel a3 of the driving device 521 is driven by the scraping blade 524, so that the large rotating wheel a3 can fix the small rotating wheel a53 in the a5 through the sliding belt a4, simultaneously the small rotating wheel a53 rotates, and the threaded rod a54 embedded in the middle part extends through the force of the positive rotation, so that the stressed block a2 can be continuously extended through the connecting head a55, the scraper 524 and the spring 523 can be continuously pressed to the original size, and then the small rotating wheel a53 can be reversed to return the parts to the original position when the spring 523 is pressed to be not pressed under the action of the sensor a51, so that the flowing speed of the steam can be effectively increased by continuously extending and contracting, and the contact area of the steam and the cell reagent can be increased. While the cellular agent can be further fused.

Example 2:

fig. 6 to 7 show:

the structure of the cell reagent adsorption device comprises that the air suction barrel 54 is provided with an adsorption shaft 541, an extractor 542, an arc-shaped plate 543 and a conduit 545, wherein the adsorption shaft 541 is installed above the extractor 542, the arc-shaped plate 543 and the adsorption shaft 541 are integrated and are positioned below the adsorption shaft 541, the conduit 545 penetrates through the arc-shaped plate 543 and is connected with the extractor 542, the conduit 545 penetrates through the middle part of the arc-shaped plate 543, the arc-shaped plate 543 can effectively guarantee the smooth operation of other parts through the shape of the arc-shaped plate 543, and the conduit 545 can block cell reagents through other parts.

The conduit 545 is provided with a solid layer b11, an extension port b12, a rotating groove b13, a push block b14 and an exhaust plate b15, the solid layer b11 and the extension port b12 are located at the same center point, the rotating groove b13 is mounted on the outer ring of the extension port b12 and rotates, the push blocks b14 are distributed on the outer ring of the rotating groove b13, the exhaust plate b15 is welded at the upper end of the push block b14, the exhaust plate b15 can prevent cell reagents from being poured into the extension port b12 through the push block b14 and the rotating groove b13, and the exhaust plate b15 can effectively exhaust steam to the bottom of the cell reagents through other components, so that the sterilization effect of the cell reagents is kept consistent.

The specific functions and operation flow of this embodiment are as follows:

in the invention, when the fermentation tank 5 continuously generates steam, the gas suction barrel 54 can continuously absorb part of the steam through the extractor 542 and simultaneously discharge the steam through the guide pipe 545, the guide pipe 545 is pushed by a large amount of steam, the rotary groove b13 can drive the gas exhaust plate b15 and the extension port b12, so that the solid layer b11 can effectively block the cell reagent under the continuous operation through the pushing block b13, the gas exhaust plate b15 discharges the steam absorbed by the extractor 542, and under the condition of the components, the bottom of the cell reagent can be effectively sterilized, and the sterilization conditions of the bottom and the top of the cell reagent are kept consistent, so that the uneven sterilization condition is not generated.

The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention to achieve the above technical effects.

Claims

1. The utility model provides a sterilization fermentation cylinder for preparing cell culture medium, its structure includes support (1), controller (2), pan feeding mouth (3), partition panel (4), fermentation cylinder (5), its characterized in that: the support (1) is fixed at the lower end of the fermentation tank (5), the controller (2) is installed at the outer ring part of the fermentation tank (5), the feeding port (3) and the partition plate (4) are located at the upper end of the partition plate (4) and are in a communicated state, and the upper end of the fermentation tank (5) is connected and communicated with the partition plate (4);

fermentation cylinder (5) inside sealing washer (51), rabbling mechanism (52), activity space (53), the bucket of breathing in (54) of being equipped with, rabbling mechanism (52) have been inlayed to sealing washer (51) middle part, activity space (53) are located the outer lane position of rabbling mechanism (52), it installs on the inner circle edge of sealing washer (51) to inhale bucket (54).

2. A sterilized fermenter for use in preparing cell culture medium according to claim 1, wherein: the stirring mechanism (52) is provided with a driving device (521), a sliding block (522), a spring (523), a scraping blade (524) and a connecting frame (525), the driving device (521) and the sliding block (522) form an opposite surface state, the spring (523) is distributed on the outer ring part of the sliding block (522), the scraping blade (524) is welded at the upper end of the spring (523) and is movably matched with the spring, and the connecting frame (525) is fixed on two sides of the scraping blade (524).

3. A sterilized fermenter for use in preparing cell culture medium according to claim 2, wherein: the driving device (521) is provided with a protective cover (a1), a stress block (a2), a large rotating wheel (a3), a sliding belt (a4) and a fixed seat (a5), wherein the protective cover (a1) is embedded in the edge position of the stress block (a2), the large rotating wheel (a3) is installed at the lower end of the protective cover (a1), the sliding belt (a4) is embedded in the middle of the protective cover (a1) and movably connected with the large rotating wheel (a3), and the fixed seat (a5) is connected with the bottom of the sliding belt (a4) and movably matched with the large rotating wheel (a 3).

4. A sterilized fermenter for use in preparing cell culture medium according to claim 3, wherein: fixing base (a5) are equipped with sensor (a51), connecting wire (a52), little runner (a53), threaded rod (a54), connector (a55), sensor (a51) installation and the below of little runner (a53) and be connected with little runner (a53) through connecting wire (a52), threaded rod (a54) imbeds in little runner (a53) inboard and carry out clearance fit, connector (a55) and threaded rod (a54) are as an organic whole.

5. A sterilized fermenter for use in preparing cell culture medium according to claim 1, wherein: the air suction barrel (54) is provided with an adsorption shaft (541), an extractor (542), an arc-shaped plate (543) and a guide pipe (545), the adsorption shaft (541) is installed above the extractor (542), the arc-shaped plate (543) and the adsorption shaft (541) are integrated and located below the adsorption shaft (541), and the guide pipe (545) penetrates through the arc-shaped plate (543) and is connected with the extractor (542).

6. A sterilized fermenter for use in preparing a cell culture medium according to claim 5, wherein: the utility model provides a pipe (545) are equipped with solid layer (b11), extend mouthful (b12), rotatory groove (b13), ejector pad (b14), exhaust plate (b15), solid layer (b11) is located same central point with extension mouthful (b12), rotatory groove (b13) are installed in the outer lane of extending mouthful (b12) and are carried out rotatory activity, ejector pad (b14) distribute in the outer lane of rotatory groove (b13), exhaust plate (b15) welds the upper end in ejector pad (b 14).