CN112353729A - Toothpaste for relieving oral ulcer and relieving swelling and pain and preparation method thereof - Google Patents
Toothpaste for relieving oral ulcer and relieving swelling and pain and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/25—Silicon; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses toothpaste for relieving dental ulcer, diminishing swelling and relieving pain and a preparation method thereof. The toothpaste is prepared from the following raw materials, by weight, 28-38 parts of calcium hydrogen phosphate dihydrate, 1-6 parts of hydrous silicic acid, 10-16 parts of glycerol, 4-10 parts of polyethylene glycol-4004, 1-5 parts of sodium carboxymethylcellulose, 0.1-0.7 part of xanthan gum, 1-6 parts of sodium dodecyl sulfate, 0.1-0.7 part of saccharin sodium, 3-10 parts of vine tea, 2-8 parts of winter pear tea, 3-12 parts of sodium phytate, 0.5-1.5 parts of menthol, 0.1-0.6 part of chitosan, 1-6 parts of calcium sodium phosphosilicate (bioactive glass) and 35-45 parts of pure water. The toothpaste is a pure natural toothpaste added with the extract of the winter pear tea and the vine tea and an antiallergic component of calcium phosphosilicate sodium, mainly aims at dental ulcer and red swelling and burning pain of gum, and has better effects of protecting and repairing ulcer, and relieving swelling and pain.
Description
Technical Field
The invention belongs to the technical field of daily necessities, and particularly relates to toothpaste capable of relieving dental ulcer and relieving swelling and pain and a preparation method thereof.
Background
At present, most of toothpaste with the effects of diminishing inflammation, relieving pain and preventing allergy in the market is added with western medicine components: hexane diclofop-p-butyl, metronidazole and the like are easy to cause normal flora imbalance in oral cavity after long-term use, and even have mutation-causing effect on serious patients.
Therefore, the technical personnel in the field need to solve the problem of how to provide toothpaste which has natural ingredients, obvious curative effect, and can relieve oral ulcer and relieve swelling and pain.
Disclosure of Invention
In view of the above, the present invention aims to overcome the above problems in the prior art, and provides a toothpaste for alleviating oral ulcer and relieving swelling and pain and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
the toothpaste for relieving dental ulcer and relieving swelling and pain is characterized by being prepared from the following raw materials in parts by weight:
28-38 parts of calcium hydrophosphate dihydrate, 1-6 parts of hydrous silicic acid, 10-16 parts of glycerol, 4-10 parts of polyethylene glycol-4004, 1-5 parts of sodium carboxymethylcellulose, 0.1-0.7 part of xanthan gum, 1-6 parts of sodium dodecyl sulfate, 0.1-0.7 part of saccharin sodium, 3-10 parts of ampelopsis grossedentata, 2-8 parts of winter pear tea, 3-12 parts of sodium phytate, 0.5-1.5 parts of menthol, 0.1-0.6 part of chitosan, 1-6 parts of calcium sodium phosphosilicate (bioactive glass) and 35-45 parts of pure water.
Preferably, the toothpaste is prepared from the following raw materials in parts by weight:
32 parts of calcium hydrophosphate dihydrate, 4 parts of hydrous silicic acid, 15 parts of glycerol, 5 parts of polyethylene glycol-4005 parts of sodium carboxymethylcellulose, 0.6 part of xanthan gum, 5 parts of sodium dodecyl sulfate, 0.4 part of saccharin sodium, 4 parts of ampelopsis grossedentata, 8 parts of winter pear tea, 5 parts of sodium phytate, 1 part of menthol, 0.5 part of chitosan, 3 parts of calcium sodium phosphosilicate (bioactive glass) and 40 parts of pure water.
The invention discloses a pure natural toothpaste added with winter pear tea, vine tea extract and antiallergic component calcium sodium phosphosilicate, wherein the winter pear tea and vine tea extract have the effects of antibiosis, antiphlogosis and pain relief, and the calcium sodium phosphosilicate has the effects of antianaphylaxis and repairing the wound surface of oral ulcer.
Specifically, the Ampelopsis grossedentata extract and the winter pear tea extract which are both extracts in tea drinks drunk by people in daily life are natural and safe, and animal experiments prove that the Ampelopsis grossedentata extract and the winter pear tea extract have certain anti-inflammatory and analgesic effects and have a nursing effect on teeth and oral cavities.
The winter pear tea is mainly distributed in Guangdong, Guangxi and Hunan provinces, and is a ancient rare plant, deciduous tree, Rosaceae, Malus. The folk habit of picking leaves to make tea drink mainly contains flavonoid, polyphenol, terpenoid, amino acid, vitamin, iron, copper, zinc and other mineral substances, and has the functions of cancer prevention, blood pressure reduction, liver protection, inflammation diminishing, pain relieving, tooth strengthening and bacteriostasis.
The Ampelopsis grossedentata is a tea-like product prepared by carrying out enzyme deactivation, rolling and drying on tender leaves of Ampelopsis grossedentata, contains abundant flavonoid compounds, phenols, polysaccharides, volatile oil, amino acids, trace elements and the like, and has the effects of resisting bacteria, diminishing inflammation, protecting liver, reducing blood sugar and blood fat and regulating immunity.
The sodium calcium phosphosilicate is an artificial composite biological material which is prepared in 1971 and takes silicate and calcium phosphate as main matrixes, can adapt to the special environment of a human body, has better biocompatibility, and has certain effect on regeneration of hard tissues of bones and teeth and treatment of dental ulcer.
The invention also aims to provide a preparation method of the toothpaste which is simple and convenient to operate and suitable for large-scale production and can relieve dental ulcer, reduce swelling and relieve pain.
A preparation method of toothpaste for relieving oral ulcer and relieving swelling and pain comprises the following steps:
(1) weighing Ampelopsis grossedentata according to a formula, crushing, decocting and filtering, and concentrating the filtrate under reduced pressure to obtain an Ampelopsis grossedentata extract;
(2) weighing the winter pear tea according to the formula, crushing, extracting under reflux, filtering, and concentrating the filtrate under reduced pressure to obtain an winter pear tea extract;
(3) wetting and uniformly mixing glycerol, sodium carboxymethylcellulose and xanthan gum, adding pure water, stirring until the prepared glue is in a uniform semitransparent viscous liquid state, then storing, and standing overnight for later use;
(4) adding calcium hydrogen phosphate dihydrate, hydrous silicic acid, polyethylene glycol-400 and sodium dodecyl sulfate into the glue prepared in the step (3), uniformly stirring and mixing, then adding saccharin sodium, the vine tea extracting solution, the winter pear tea extracting solution, sodium phytate, menthol, chitosan and calcium sodium phosphosilicate (bioactive glass), and stirring to obtain a paste;
(5) and (5) homogenizing the paste prepared in the step (4) and canning to obtain the toothpaste for relieving the dental ulcer and relieving swelling and pain.
Preferably, in the step (1), the crushed particle size is 60-100 meshes, and 5-10 times of water is added for decocting for 2-3 hours, and then the filtrate is obtained by filtering in 100-200 meshes.
Further preferably, the vacuum degree during the reduced pressure concentration is-0.07 to-0.08 Mpa, the temperature is 60 ℃ to 70 ℃, and the filtrate is concentrated to the liquid medicine with the relative density of 1.02 to 1.06, namely the vine tea extract.
Preferably, in the step (2), the crushed particles with the particle size of 60-100 meshes are added with 5-10 times of 50-80% ethanol for heating reflux extraction, and then the mixture is filtered through 100-200 meshes to obtain the filtrate.
Preferably, the temperature of the water bath during the reflux extraction is 60-85 ℃, and the filtrate is concentrated to a liquid medicine with the relative density of 1.02-1.06, namely the winter pear tea extract.
Preferably, the stirring time in the step (4) is 30-60 minutes, and the rotation speed is 200--1。
Preferably, in the step (5), the number of homogenizations is 2-3, and the vacuum degassing time is 30-60 min.
According to the technical scheme, compared with the prior art, the invention discloses and provides the toothpaste for relieving oral ulcer and relieving swelling and pain and the preparation method thereof, and the toothpaste has the following excellent effects:
the product does not contain antibiotics and hormone chemical drugs, mainly contains pure natural plant extracts, wherein the vine tea extract has obvious anti-inflammatory effect, the winter pear tea extract has good pain relieving effect, and the natural biological composite material bioactive glass is added to play a role in diminishing inflammation, relieving pain and relieving dental ulcer, so that the product has no side effect, and is suitable for popularization and application in the market.
Detailed Description
The technical solutions disclosed in the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses toothpaste for relieving oral ulcer and relieving swelling and pain and a preparation method thereof.
The present invention will be further specifically illustrated by the following examples for better understanding, but the present invention is not to be construed as being limited thereto, and certain insubstantial modifications and adaptations of the invention by those skilled in the art based on the foregoing disclosure are intended to be included within the scope of the invention.
The technical solution disclosed in the present invention will be further described with reference to the following specific examples.
Screening experiment of prescription ratio
Example 1:
32 parts of calcium hydrophosphate dihydrate, 4 parts of hydrous silicic acid, 15 parts of glycerol, 5 parts of polyethylene glycol-4005 parts of sodium carboxymethylcellulose, 0.6 part of xanthan gum, 5 parts of sodium dodecyl sulfate, 0.4 part of saccharin sodium, 4 parts of ampelopsis grossedentata, 8 parts of winter pear tea, 5 parts of sodium phytate, 1 part of menthol, 0.5 part of chitosan, 3 parts of calcium sodium phosphosilicate (bioactive glass) and 40 parts of pure water.
Example 2:
28 parts of calcium hydrophosphate dihydrate, 2 parts of hydrous silicic acid, 10 parts of glycerol, 4004 parts of polyethylene glycol, 2 parts of sodium carboxymethylcellulose, 0.1 part of xanthan gum, 1 part of sodium dodecyl sulfate, 0.1 part of saccharin sodium, 3 parts of ampelopsis grossedentata, 2 parts of winter pear tea, 3 parts of sodium phytate, 0.5 part of menthol, 0.1 part of chitosan, 1 part of calcium sodium phosphosilicate (bioactive glass) and 35 parts of pure water.
Example 3:
35 parts of calcium hydrophosphate dihydrate, 5 parts of hydrous silicic acid, 16 parts of glycerol, 8 parts of polyethylene glycol-4008 parts of sodium carboxymethylcellulose, 0.5 part of xanthan gum, 3 parts of sodium dodecyl sulfate, 0.5 part of saccharin sodium, 5 parts of ampelopsis grossedentata, 5 parts of winter pear tea, 8 parts of sodium phytate, 1.2 parts of menthol, 0.6 part of chitosan, 5 parts of calcium sodium phosphosilicate (bioactive glass) and 45 parts of pure water.
Preference of preparation Process
Example 4:
a preparation method of toothpaste for relieving oral ulcer and relieving swelling and pain comprises the following steps:
(1) weighing vine tea according to a formula, crushing, sieving by a 60-mesh sieve, adding 6 times of water for decocting for 2 hours, filtering by a 100-mesh sieve, and concentrating the filtrate under reduced pressure (the vacuum degree is-0.07 to-0.08 Mpa) to obtain a medicinal liquid with the relative density of 1.02 to obtain a vine tea extracting solution;
(2) weighing the winter pear tea according to a formula, crushing, sieving by a 60-mesh sieve, adding 6 times of 50% ethanol, performing reflux extraction for 2 hours at the water bath temperature of 60 ℃, filtering by a 100-mesh sieve, and concentrating the filtrate under reduced pressure (the vacuum degree is-0.07 to-0.08 Mpa) to obtain a liquid medicine with the relative density of 1.02 to obtain a winter pear tea extract;
(3) wetting and uniformly mixing glycerol, sodium carboxymethylcellulose and xanthan gum, adding pure water, stirring for 30 minutes at the rotation speed of 500 r.min-1So that the glue producedWater is in a uniform semitransparent sticky liquid state, and then the water is stored and placed overnight for standby;
(4) adding calcium hydrogen phosphate dihydrate, hydrous silicic acid, polyethylene glycol-400 and sodium dodecyl sulfate into the glue prepared in the step (3), uniformly stirring and mixing, then adding saccharin sodium, the vine tea extracting solution, the winter pear tea extracting solution, sodium phytate, menthol, chitosan and calcium sodium phosphosilicate (bioactive glass), and stirring to obtain a paste;
(5) homogenizing the paste prepared in the step (4) for 2 times, vacuum degassing for 30 minutes, and canning to obtain the toothpaste for relieving the dental ulcer and relieving swelling and pain.
Example 5:
a preparation method of toothpaste for relieving oral ulcer and relieving swelling and pain comprises the following steps:
(1) weighing vine tea according to a formula, crushing, sieving by a 80-mesh sieve, adding 8 times of water, decocting for 2.5 hours, filtering by a 150-mesh sieve, and concentrating the filtrate under reduced pressure (the vacuum degree is-0.07 to-0.08 Mpa) to obtain a medicinal liquid with the relative density of 1.04 to obtain a vine tea extracting solution;
(2) weighing the winter pear tea according to the formula, crushing, sieving by a sieve of 80 meshes, adding 8 times of 70% ethanol, carrying out reflux extraction for 2.5 hours at the water bath temperature of 70 ℃, filtering, and concentrating the filtrate under reduced pressure (the vacuum degree is-0.07 to-0.08 Mpa) to obtain a liquid medicine with the relative density of 1.04 to obtain a winter pear tea extract;
(3) wetting and uniformly mixing glycerol, sodium carboxymethylcellulose and xanthan gum, adding pure water, stirring for 45 minutes at the rotating speed of 300 r.min-1So that the prepared glue is in a uniform, semitransparent and sticky liquid state, and then is stored and placed overnight for standby;
(4) adding calcium hydrogen phosphate dihydrate, hydrous silicic acid, polyethylene glycol-400 and sodium dodecyl sulfate into the glue prepared in the step (3), uniformly stirring and mixing, then adding saccharin sodium, the vine tea extracting solution, the winter pear tea extracting solution, sodium phytate, menthol, chitosan and calcium sodium phosphosilicate (bioactive glass), and stirring to obtain a paste;
(5) homogenizing the paste prepared in the step (4) for 3 times, vacuum degassing for 45 minutes, and canning to obtain the toothpaste for relieving the dental ulcer and relieving swelling and pain.
Example 6:
a preparation method of toothpaste for relieving oral ulcer and relieving swelling and pain comprises the following steps:
(1) weighing vine tea according to a formula, crushing, sieving by a 100-mesh sieve, adding 10 times of water, decocting for 3 hours, filtering by a 200-mesh sieve, and concentrating the filtrate under reduced pressure (the vacuum degree is-0.07 to-0.08 Mpa) to obtain a medicinal liquid with the relative density of 1.06 to obtain a vine tea extracting solution;
(2) weighing the winter pear tea according to the formula, crushing, sieving by a 100-mesh sieve, adding 10 times of 80% ethanol, carrying out reflux extraction for 3 hours at the water bath temperature of 80 ℃, filtering, and concentrating the filtrate under reduced pressure (the vacuum degree is-0.07 to-0.08 Mpa) to obtain a liquid medicine with the relative density of 1.06 to obtain a winter pear tea extract;
(3) wetting and uniformly mixing glycerol, sodium carboxymethylcellulose and xanthan gum, adding pure water, stirring for 60 minutes at the rotation speed of 200 r.min-1So that the prepared glue is in a uniform, semitransparent and sticky liquid state, and then is stored and placed overnight for standby;
(4) adding calcium hydrogen phosphate dihydrate, hydrous silicic acid, polyethylene glycol-400 and sodium dodecyl sulfate into the glue prepared in the step (3), uniformly stirring and mixing, then adding saccharin sodium, the vine tea extracting solution, the winter pear tea extracting solution, sodium phytate, menthol, chitosan and calcium sodium phosphosilicate (bioactive glass), and stirring to obtain a paste;
(5) homogenizing the paste prepared in the step (4) for 3 times, vacuum degassing for 60 minutes, and canning to obtain the toothpaste for relieving the dental ulcer and relieving swelling and pain.
The inventive content is not limited to the content of the above-mentioned embodiments, wherein combinations of one or several of the embodiments may also achieve the object of the invention.
In order to verify the excellent effects of the toothpaste disclosed and protected by the invention for relieving dental ulcer and relieving swelling and pain, the experiment on ear swelling of a mouse subjected to test substance p-xylene-induced inflammation shows that the vine tea extract has an obvious anti-inflammatory effect compared with a winter pear tea extract, and can well eliminate ear swelling of the mouse; the mouse writhing experiment result shows that the winter pear tea extract has a remarkable inhibiting effect on the mouse writhing reaction caused by acetic acid; the 2 extracts are used together, and have synergistic effect and enhanced analgesic effect. The specific experimental procedures were as follows:
anti-inflammatory and analgesic test of vine tea extract and Chinese pear tea extract
1 materials of the experiment
1.1 test article
Ampelopsis grossedentata extract (light brown powder) and Pyrus pyrifolia extract (dark brown powder) provided by the Xieei teacher of the institute of traditional Chinese medicine, Hunan province.
1.2 Experimental animals
90 male ICR mice, the weight of 18.0-22.0 g, animal qualification number: no.430727201100949367, purchased from slai kholda laboratory animals ltd, Hunan, laboratory animals production license no: SCXK (Xiang) 2019-; the animal breeding method is characterized in that the animal is bred in a barrier environment in an animal room of a traditional Chinese medicine research institute in Hunan province, and the experimental animals use license numbers: SYXK (Xiang) 2020-.
1.3 Primary reagents
Xylene (030518, a product of Fuyu Fine chemical Co., Ltd., Tianjin); glacial acetic acid (lot No. 20170106, chemical reagents of national drug group, ltd.) was prepared into a 0.6% acetic acid solution with physiological saline immediately before use.
1.4 Main Instrument
Electronic balance, analytical balance (mettler-toledo).
2 method of experiment
2.1 test substance-Paraxylinemia mouse ear swelling experiment
2.1.1 grouping and administration
Selecting 40 male ICR mice qualified for quarantine, weighing 18.0-22.0 g, randomly dividing into 4 groups according to the weight, and each group comprises 10 mice, namely a model control group, a vine tea extract group (1.59g of dry extract powder/kg), a winter pear tea extract group (0.92g of dry extract powder/kg), and a vine tea and winter pear tea extract group (1.59g +0.92g of dry extract powder/kg). Before daily administration, purified water is adopted to prepare a test substance into test solution with corresponding concentration according to dosage, the test solution is used when the test solution is prepared, a mouse is used for intragastric administration according to 20mL/kg, administration is carried out for 1 time/day continuously for 14 days, and purified water with the same volume is administered by intragastric administration to a model control group. 1h after the last administration, the lateral side of the right ear of each animal was coated with xylene-induced inflammation (25. mu.L/animal), the left ear was not coated with xylene as a control, the animal was sacrificed after 30min, both ears were cut off along the base line of the auricle, a disk of the same portion of the left ear and the right ear was punched out with a punch having a diameter of 6mm, the weight of the disk of both ears was immediately accurately weighed on an analytical balance, and the swelling degree and swelling rate were calculated.
2.1.2 detection index
Swelling degree-right ear weight-left ear weight;
swelling rate ═ weight of right ear-weight of left ear)/weight of left ear × 100%.
2.2 test of mouse writhing caused by acetic acid
2.2.1 grouping and administration
Selecting 40 male ICR mice qualified for quarantine, weighing 18.0-22.0 g, randomly dividing into 4 groups according to the weight, and each group comprises 10 mice, namely a model control group, a vine tea extract group (1.59g of dry extract powder/kg), a winter pear tea extract group (0.92g of dry extract powder/kg), and a vine tea and winter pear tea extract group (1.59g +0.92g of dry extract powder/kg). Before daily administration, purified water is adopted to prepare a test substance into test solution with corresponding concentration according to dosage, the test solution is used when the test solution is prepared, a mouse is used for intragastric administration according to 20mL/kg, administration is carried out for 1 time/day continuously for 14 days, and purified water with the same volume is administered by intragastric administration to a model control group. 1h after the last administration, each group of mice was intraperitoneally injected with 0.6% glacial acetic acid at a dose of 0.4mL, and the number of writhing reactions of the mice within 15min after the injection of 0.6% glacial acetic acid was counted.
2.2.2 detection index
The inhibition rate of writhing reaction is (number of writhing in blank group-number of writhing in administration group)/number of writhing in blank group × 100%.
2.3 statistical methods
Statistical analysis was performed using SPSS16.0 with the level of statistical significance set to P<0.05, the average plus or minus standard deviation is adopted for the measurement data
Showing that the method of Leven's test is used to check the normality and the homogeneity of variance, if the normality and the homogeneity of variance (P) are met>0.05), statistical analysis was performed using one-way analysis of variance (ANOVA) and LSD test; if the normality and homogeneity of variance (P ≦ 0.05) are met, the test is performed by Kruskal-WallisIf the Kruskal-Wallis Test is statistically significant (P.ltoreq.0.05), a comparative analysis is carried out using Dunnett's Test (nonparametric method), taking into account the statistical differences and the biological significance in the evaluation.
3 results of the experiment
3.1 test substance-Paraxylene-induced ear swelling test in mice
As shown in table 1, compared with the model control group, the ear swelling degrees of animals in the vine tea extract group, the winter pear tea extract group, and the vine tea and winter pear tea extract group are all significantly reduced (P <0.01), and the ear swelling rate of each administration group is significantly lower than that of the model control group (P < 0.01). The results show that the test substance has obvious inhibition effect on the swelling degree and swelling rate of mouse auricles caused by the paraxylene.
TABLE 1 Effect of test substances on ear swelling experiments in P-xylene-induced inflammatory mice: (
n＝10)
Note: p <0.05, P <0.01 compared to model control.
3.2 test substance to acetic acid induced writhing in mice
As shown in table 2, compared with the model control group, the animal wriggle times of the vine tea extract group, the winter pear tea extract group, the vine tea and winter pear tea extract group are all reduced to different degrees, the wriggle reaction inhibition rates are respectively 27.94%, 44.12% and 51.47%, wherein the wriggle times of the winter pear tea extract group and the vine tea and winter pear tea extract group are significantly reduced (P < 0.01). The results show that the tested substance has an inhibiting effect on the mouse writhing reaction caused by acetic acid, wherein the effect of combining the winter pear tea extract and 2 patients is obvious.
TABLE 2 Effect of test substances on acetic acid-induced writhing in mice: (
n＝10)
Note: p <0.05, P <0.01 compared to model control.
4 summary of the experiment
The analysis shows that the vine tea extract and the Chinese pear tea extract both have obvious anti-inflammatory effect, and in addition, the Chinese pear tea extract also has certain analgesic effect.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. The toothpaste for relieving dental ulcer and relieving swelling and pain is characterized by being prepared from the following raw materials in parts by weight:
28-38 parts of calcium hydrophosphate dihydrate, 1-6 parts of hydrous silicic acid, 10-16 parts of glycerol, 4-10 parts of polyethylene glycol-4004, 1-5 parts of sodium carboxymethylcellulose, 0.1-0.7 part of xanthan gum, 1-6 parts of sodium dodecyl sulfate, 0.1-0.7 part of saccharin sodium, 3-10 parts of ampelopsis grossedentata, 2-8 parts of winter pear tea, 3-12 parts of sodium phytate, 0.5-1.5 parts of menthol, 0.1-0.6 part of chitosan, 1-6 parts of calcium sodium phosphosilicate (bioactive glass) and 35-45 parts of pure water.
2. The toothpaste for relieving oral ulcer and relieving swelling and pain as claimed in claim 1, wherein the toothpaste is prepared from the following raw materials in parts by weight:
32 parts of calcium hydrophosphate dihydrate, 4 parts of hydrous silicic acid, 15 parts of glycerol, 5 parts of polyethylene glycol-4005 parts of sodium carboxymethylcellulose, 0.6 part of xanthan gum, 5 parts of sodium dodecyl sulfate, 0.4 part of saccharin sodium, 4 parts of ampelopsis grossedentata, 8 parts of winter pear tea, 5 parts of sodium phytate, 1 part of menthol, 0.5 part of chitosan, 3 parts of calcium sodium phosphosilicate (bioactive glass) and 40 parts of pure water.
3. The preparation method of the toothpaste for relieving oral ulcer and relieving swelling and pain as claimed in any one of claims 1 to 2, wherein the method comprises the following steps:
(1) weighing Ampelopsis grossedentata according to a formula, crushing, decocting and filtering, and concentrating the filtrate under reduced pressure to obtain an Ampelopsis grossedentata extract;
(2) weighing the winter pear tea according to the formula, crushing, extracting under reflux, filtering, and concentrating the filtrate under reduced pressure to obtain an winter pear tea extract;
(3) wetting and uniformly mixing glycerol, sodium carboxymethylcellulose and xanthan gum, adding pure water, stirring until the prepared glue is in a uniform semitransparent viscous liquid state, then storing, and standing overnight for later use;
(4) adding calcium hydrogen phosphate dihydrate, hydrous silicic acid, polyethylene glycol-400 and sodium dodecyl sulfate into the glue prepared in the step (3), uniformly stirring and mixing, then adding saccharin sodium, the vine tea extracting solution, the winter pear tea extracting solution, sodium phytate, menthol, chitosan and calcium sodium phosphosilicate (bioactive glass), and stirring to obtain a paste;
(5) and (5) homogenizing the paste prepared in the step (4) and canning to obtain the toothpaste for relieving the dental ulcer and relieving swelling and pain.
4. The method for preparing toothpaste capable of alleviating oral ulcer and relieving swelling and pain as claimed in claim 3, wherein in step (1), the pulverized particles with size of 60-100 meshes are added with 5-10 times of water and decocted for 2-3 hours, and filtered through 100-200 meshes to obtain the filtrate.
5. The method for preparing toothpaste for relieving oral ulcer and relieving swelling and pain as claimed in claim 3 or 4, wherein the vacuum degree during vacuum concentration is-0.07 to-0.08 MPa, the temperature is 60 ℃ to 70 ℃, and the filtrate is concentrated to a liquid medicine with a relative density of 1.02 to 1.06, namely the Ampelopsis grossedentata extract.
6. The method as claimed in claim 3, wherein in the step (2), the crushed particles with a size of 60-100 mesh are extracted by heating and refluxing with 5-10 times of 50-80% ethanol, and then filtered through 100-200 mesh to obtain the filtrate.
7. The preparation method of the toothpaste for relieving oral ulcer and relieving swelling and pain according to claim 6, wherein the temperature of water bath during reflux extraction is 60-85 ℃, and the filtrate is concentrated to liquid medicine with the relative density of 1.02-1.06, namely the winter pear tea extract.
8. The method for preparing toothpaste for relieving oral ulcer and alleviating swelling and pain as claimed in claim 3, wherein the stirring time in step (4) is 30-60 minutes, and the rotation speed is 200--1。
9. The method for preparing toothpaste for alleviating oral ulcer and relieving swelling and pain as claimed in claim 3, wherein in the step (5), the number of homogenizations is 2-3, and the vacuum degassing time is 30-60 min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114533611A (en) * | 2022-02-16 | 2022-05-27 | 重庆登康口腔护理用品股份有限公司 | Composition capable of enhancing dentin sensitivity resistance effect of bioactive material and preparation method and application thereof |
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| CN114533611A (en) * | 2022-02-16 | 2022-05-27 | 重庆登康口腔护理用品股份有限公司 | Composition capable of enhancing dentin sensitivity resistance effect of bioactive material and preparation method and application thereof |
| CN114533611B (en) * | 2022-02-16 | 2023-08-29 | 重庆登康口腔护理用品股份有限公司 | Composition capable of enhancing dentin sensitivity resisting effect of bioactive material and preparation method and application thereof |
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