CN112336834A

CN112336834A - Plant prebiotics gel and preparation method and application thereof

Info

Publication number: CN112336834A
Application number: CN202011223863.7A
Authority: CN
Inventors: 冯驯; 陈军
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-05
Filing date: 2020-11-05
Publication date: 2021-02-09

Abstract

The invention is applicable to the technical field of medical use, and provides a plant prebiotics gel, a preparation method and an application thereof, wherein the plant prebiotics gel comprises the following components: water, glycerin, poloxamer 407, osthole, garlic extract, prebiotics nutrient, zedoary turmeric oil, cyrtomium rhizome extract, arnebia root extract, melaleuca alternifolia leaf oil, rosemary leaf oil, hairyvein agrimonia herb extract, lavender oil, matrine, chlorhexidine gluconate and lactic acid. The plant prebiotics gel provided by the invention can promote the proliferation of vaginal probiotics such as lactobacillus, lactobacillus acidophilus, bifidobacterium and the like by adopting prebiotics nutrient, and overcomes the defect that the directly added probiotics are easy to inactivate; the plant prebiotics gel has the effects of balancing vaginal microbial flora and increasing vaginal muscle elasticity, is comfortable, non-irritating, safe and reliable, and is suitable for long-term use.

Description

Plant prebiotics gel and preparation method and application thereof

Technical Field

The invention belongs to the technical field of medical use, and particularly relates to a plant prebiotic gel and a preparation method and application thereof.

Background

The vaginal part is an open and moist part, due to factors such as clothes, trousers, water quality and the like, bacteria or germs are easy to invade to cause abnormal leucorrhea, peculiar smell, fungal infection and the like, and the imbalance of vaginal flora means that the immunologic function is reduced.

In recent years, vaginal inflammation has become a threat to the reproductive health of thousands of women. OTC medicines are generally adopted in the market for treatment, such as clotrimazole, econazole, miconazole nitrate, polymetaphrenol sulfoaldehyde and other antibiotic raw material medicines, or traditional Chinese medicine anti-inflammatory products, such as radix sophorae flavescentis, fructus cnidii and the like, and some antibacterial agents, such as chlorhexidine acetate, chlorhexidine gluconate, silver ions and the like. The use of antibiotics can rapidly kill bacteria and eliminate vaginal inflammation to a certain extent. However, broad-spectrum antibiotics can kill bacteria and cause overgrowth of vaginal fungi; the longer the antibiotic is used, the higher the possibility of fungal infection; some traditional Chinese medicines and antibacterial agents have certain killing effect on fungi; however, both the antibiotic and the antibiotic have a fatal defect, harmful bacteria are killed, beneficial bacteria are killed, vaginal flora balance is disturbed, the immunity of the vaginal environment is rapidly reduced, and secondary infection of fungi and bacteria is easy to occur.

The probiotics can secrete organic acid, can maintain the system in an acidic environment, and inhibit the proliferation of harmful bacteria such as staphylococcus aureus, candida albicans and the like through competitive advantages so as to maintain the vaginal balance of new flora.

In order to maintain the balance of vaginal flora, it has also been reported that probiotics such as lactobacillus are directly implanted into vaginal care products to achieve the purpose of inhibiting the growth of fungi and bacteria. However, the lactobacillus needs to have high requirements on environmental cleanliness and temperature when being manufactured, transported and stored, the suitable growth temperature of the strains is 30-35 ℃, cold-chain transportation is needed, the storage requirement is high, if the lactobacillus is easy to lose activity without paying attention to sanitation and temperature, the effect cannot be released, and the original inflammation problem is aggravated after the lactobacillus is used.

Vaginal relaxation is caused by the fact that elastic fibers in muscles (including anal sphincter, levator ani and bulbocavernosus) are broken due to age, childbirth or trauma, so that an elastic fiber net of the vagina is damaged, the contraction force of the vagina is reduced, and the relaxed vagina is easy to have residual germ mycin, so that a series of gynecological problems occur.

Therefore, the development of a product for scientifically and efficiently promoting the proliferation of vaginal probiotics to maintain the balance of vaginal flora and increase the elasticity of vaginal muscles is urgent.

Disclosure of Invention

The embodiment of the invention aims to provide a plant prebiotic gel, aiming at solving the problems in the background art.

The embodiment of the invention is realized in such a way that the plant prebiotics gel comprises the following components in parts by weight: 60-80 parts of water, 10-20 parts of glycerol, 40715-20 parts of poloxamer, 0.1-0.5 part of osthole, 2-5 parts of garlic extract, 1-5 parts of prebiotics nutrient, 1-8 parts of zedoary turmeric oil, 3-6 parts of cyrtomium rhizome extract, 3-10 parts of sinkiang arnebia root extract, 0.1-1 part of alternanthera leaf oil, 0.1-1 part of rosemary leaf oil, 1-5 parts of hairyvein agrimonia herb extract, 0.1-2 parts of lavender oil, 0.1-0.5 part of matrine, 0.06-0.3 part of chlorhexidine gluconate and 0.1-0.5 part of lactic acid.

As a preferred scheme of the embodiment of the invention, the plant prebiotic gel comprises the following components in parts by weight: 65-68 parts of water, 12-15 parts of glycerol, 40716-18 parts of poloxamer, 0.2-0.3 part of osthole, 2.5-3 parts of garlic extract, 4-4.5 parts of prebiotics nutrient, 1.5-2 parts of zedoary turmeric oil, 3.2-4 parts of cyrtomium rhizome extract, 5.1-6 parts of Sinkiang arnebia root extract, 0.4-0.5 part of alternanthera leaf oil, 0.25-0.5 part of rosemary leaf oil, 2-3 parts of hairyvein agrimonia herb extract, 0.5-1 part of lavender oil, 0.3-0.4 part of matrine, 0.25-0.3 part of chlorhexidine gluconate and 0.25-0.3 part of lactic acid.

In another preferred embodiment of the present invention, the prebiotic nutrient is at least one of inulin, α -glucan oligosaccharide, galacto-oligosaccharide, and isomalto-oligosaccharide.

Another object of the embodiments of the present invention is to provide a method for preparing the above plant prebiotic gel, which includes the following steps:

weighing water, glycerol, poloxamer 407, osthole, garlic extract, prebiotics nutrient, zedoary turmeric oil, cyrtomium rhizome extract, Sinkiang arnebia root extract, melaleuca alternifolia leaf oil, rosemary leaf oil, hairyvein agrimonia herb and bud extract, lavender oil, matrine, chlorhexidine gluconate and lactic acid according to the parts by weight of the components;

boiling water, cooling, and mixing poloxamer 407 and glycerol to obtain a mixed solution;

under the condition of stirring, adding osthole, garlic extract, prebiotics nutrient solution, zedoary turmeric oil, cyrtomium rhizome extract, Sinkiang arnebia root extract, alternanthera leaf and cajeput leaf oil, rosemary leaf oil, hairyvein agrimony extract, lavender oil, matrine and chlorhexidine gluconate into the mixed solution in sequence, mixing, and adding lactic acid to adjust the pH value to obtain the plant prebiotics gel.

As another preferable scheme of the embodiment of the present invention, in the step, the method of cooking water comprises: and heating the water to 90-95 ℃, preserving the heat for 30-35 minutes, and then cooling to room temperature.

As another preferable scheme of the embodiment of the invention, in the step, the temperature for mixing the boiled water, the poloxamer 407 and the glycerol is 8-12 ℃.

In another preferable embodiment of the invention, the pH of the plant prebiotic gel is 4-4.5.

Another object of the embodiment of the invention is to provide a plant prebiotic gel prepared by the preparation method.

Another object of an embodiment of the present invention is to provide a use of the above plant prebiotic gel in the preparation of a formulation for balancing vaginal microbial flora and/or increasing vaginal muscle elasticity.

According to the plant prebiotic gel provided by the embodiment of the invention, the prebiotic nutrient is adopted to promote the proliferation of the vaginal probiotics such as lactobacillus, lactobacillus acidophilus, bifidobacterium and the like, so that the defect that the inactivation is easy when the probiotics are directly added is overcome; in addition, the gel is prepared from prebiotic nutrients and a plurality of plant extract components, wherein the prebiotic nutrients can promote the proliferation of beneficial bacteria in the vagina and enable the vagina to be in an acidic environment of 3.8-4.5; the cnidium lactone, the garlic extract, the zedoary turmeric oil, the cyrtomium rhizome extract, the Sinkiang arnebia root extract, the melaleuca alternifolia leaf oil, the rosemary leaf oil, the hairyvein agrimonia herb and bud extract, the lavender oil, the matrine and other plant extracts and the antibacterial agent chlorhexidine gluconate are scientifically mixed, the bacteriostasis rate of harmful microorganisms (bacteria and fungi) is up to more than 99.99 percent, the influence on beneficial bacteria is small (less than 10 percent in 24 h), the balance of vaginal microbial flora can be maintained, the vaginal muscle loss can be assisted and repaired, and the vaginal relaxation can be improved. In conclusion, the plant prebiotics gel provided by the invention has the effects of balancing vaginal microbial flora and increasing vaginal muscle elasticity, is comfortable, non-irritating, safe and reliable, and is suitable for long-term use.

Detailed Description

In order to clearly and completely describe the technical solutions in the embodiments of the present invention in combination with the embodiments of the present invention, it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

This embodiment provides a plant prebiotic gel, the method of preparation comprising the steps of:

s1, weighing 65 parts of water, 12 parts of glycerol, 40716 parts of poloxamer, 0.2 part of osthole, 3 parts of garlic extract, 4.0 parts of prebiotics nutrient, 2 parts of zedoary turmeric oil, 4.0 parts of cyrtomium rhizome extract, 6.0 parts of Sinkiang arnebia root extract, 0.5 part of melaleuca alternifolia leaf oil, 0.5 part of rosemary leaf oil, 3.0 parts of hairyvein agrimonia herb extract, 1.0 part of lavender oil, 0.4 part of matrine, 0.25 part of chlorhexidine gluconate and 0.25 part of lactic acid for later use; wherein the prebiotic nutrient is inulin.

S2, adding the weighed water into a pot, heating to 90 ℃, preserving heat for 30 minutes, and then cooling to room temperature.

S3, adding the weighed poloxamer 407 and glycerol into the pot, cooling to 10 ℃, mixing, and simultaneously rapidly stirring to completely dissolve the poloxamer to obtain a mixed solution.

S4, sequentially adding osthole, garlic extract, prebiotics nutrient solution, zedoary turmeric oil, cyrtomium rhizome extract, Sinkiang arnebia root extract, melaleuca alternifolia leaf oil, rosemary leaf oil, hairyvein agrimonia herb extract, lavender oil, matrine and chlorhexidine gluconate into the mixed solution under the condition of stirring, stirring until the mixture is completely dissolved, adding lactic acid to adjust the pH value to 4.2, and stirring to prepare gel, thus obtaining the plant prebiotics gel which can balance vaginal microbial flora and increase vaginal muscle elasticity.

Example 2

s1, weighing 68 parts of water, 15 parts of glycerol, 40718 parts of poloxamer, 0.3 part of osthole, 2.5 parts of garlic extract, 4.5 parts of prebiotics nutrient, 1.5 parts of zedoary turmeric oil, 3.2 parts of cyrtomium rhizome extract, 5.1 parts of sinkiang arnebia root extract, 0.4 part of melaleuca alternifolia leaf oil, 0.25 part of rosemary leaf oil, 2.0 parts of hairyvein agrimonia herb extract, 0.5 part of lavender oil, 0.3 part of matrine, 0.25 part of chlorhexidine gluconate and 0.3 part of lactic acid for later use; wherein the prebiotic nutrient is alpha-glucan oligosaccharide.

S2, adding the weighed water into the pot, heating to 92 ℃, preserving heat for 35 minutes, and then cooling to room temperature.

S3, adding the weighed poloxamer 407 and glycerol into the pot, cooling to 8 ℃, mixing, and simultaneously rapidly stirring to completely dissolve the poloxamer to obtain a mixed solution.

Example 3

s1, weighing 60 parts of water, 15 parts of glycerol, 40716 parts of poloxamer, 0.3 part of osthole, 2.5 parts of garlic extract, 3.0 parts of inulin, 2.0 parts of alpha-glucan oligosaccharide, 1 part of zedoary turmeric oil, 3 parts of cyrtomium rhizome extract, 5.1 parts of arnebia root extract, 0.4 part of alternanthera leaf oil, 0.6 part of rosemary leaf oil, 5.0 parts of hairyvein agrimonia herb extract, 0.5 part of lavender oil, 0.3 part of matrine, 0.3 part of chlorhexidine gluconate and 0.3 part of lactic acid for later use; wherein the prebiotic nutrient is a mixture of inulin and alpha-glucan oligosaccharide.

Example 4

s1, weighing 79 parts of water, 15 parts of glycerol, 40716 parts of poloxamer, 0.3 part of osthole, 4.0 parts of garlic extract, 1.5 parts of inulin, 2.0 parts of alpha-glucan oligosaccharide, 1.5 parts of galacto-oligosaccharide, 1 part of zedoary turmeric oil, 4 parts of cyrtomium rhizome extract, 4 parts of Sinkiang arnebia root extract, 0.5 part of interbedded leaf cajeput oil, 0.6 part of rosemary leaf oil, 5.0 parts of hairyvein agrimonia herb extract, 0.4 part of lavender oil, 0.32 part of matrine, 0.28 part of chlorhexidine gluconate and 0.3 part of lactic acid for later use; wherein the prebiotic nutrient is a mixture of inulin, alpha-glucan oligosaccharide and galacto-oligosaccharide.

S4, sequentially adding osthole, garlic extract, prebiotics nutrient solution, zedoary turmeric oil, cyrtomium rhizome extract, Sinkiang arnebia root extract, melaleuca alternifolia leaf oil, rosemary leaf oil, hairyvein agrimonia herb extract, lavender oil, matrine and chlorhexidine gluconate into the mixed solution under the condition of stirring, stirring until the mixture is completely dissolved, adding lactic acid to adjust the pH value to 4.3, and stirring to prepare gel, thus obtaining the plant prebiotics gel which can balance vaginal microbial flora and increase vaginal muscle elasticity.

Example 5

s1, weighing 68 parts of water, 12 parts of glycerol, 40718 parts of poloxamer, 0.5 part of osthole, 4.0 parts of garlic extract, 1.8 parts of inulin, 1.5 parts of alpha-glucan oligosaccharide, 1.0 part of isomaltooligosaccharide, 5 parts of zedoary turmeric oil, 4 parts of cyrtomium rhizome extract, 6 parts of Sinkiang arnebia root extract, 0.8 part of alternanthera leaf and cajeput leaf oil, 0.7 part of rosemary leaf oil, 4.0 parts of hairyvein agrimonia herb extract, 1.2 parts of lavender oil, 0.3 part of matrine, 0.25 part of chlorhexidine gluconate and 0.4 part of lactic acid for later use; wherein the prebiotic nutrient is a mixture of inulin, alpha-glucan oligosaccharide and isomaltooligosaccharide.

S3, adding the weighed poloxamer 407 and glycerol into the pot, cooling to 12 ℃, mixing, and simultaneously rapidly stirring to completely dissolve the poloxamer to obtain a mixed solution.

S4, sequentially adding osthole, garlic extract, prebiotics nutrient solution, zedoary turmeric oil, cyrtomium rhizome extract, Sinkiang arnebia root extract, melaleuca alternifolia leaf oil, rosemary leaf oil, hairyvein agrimonia herb and bud extract, lavender oil, matrine and chlorhexidine gluconate into the mixed solution under stirring, stirring until the components are completely dissolved, adding lactic acid to adjust the pH value to 4, and stirring to prepare gel, thus obtaining the plant prebiotics gel which can balance vaginal microbial flora and increase vaginal muscle elasticity.

Example 6

s1, weighing 70 parts of water, 20 parts of glycerol, 40720 parts of poloxamer, 0.5 part of osthole, 5.0 parts of garlic extract, 2.0 parts of inulin, 1.0 part of alpha-glucan oligosaccharide, 1.0 part of galacto-oligosaccharide, 1.0 part of isomaltooligosaccharide, 6 parts of zedoary turmeric oil, 5 parts of cyrtomium rhizome extract, 8 parts of Sinkiang lithospermum extract, 0.9 part of melaleuca alternifolia leaf oil, 0.8 part of rosemary leaf oil, 5.0 parts of hairyvein agrimonia herb extract, 1.5 parts of lavender oil, 0.4 part of matrine, 0.3 part of chlorhexidine gluconate and 0.32 part of lactic acid for later use; wherein the prebiotic nutrient is a mixture of inulin, alpha-glucan oligosaccharide, galacto-oligosaccharide and isomalto-oligosaccharide.

S4, sequentially adding osthole, garlic extract, prebiotics nutrient solution, zedoary turmeric oil, cyrtomium rhizome extract, Sinkiang arnebia root extract, melaleuca alternifolia leaf oil, rosemary leaf oil, hairyvein agrimonia herb extract, lavender oil, matrine and chlorhexidine gluconate into the mixed solution under the condition of stirring, stirring until the mixture is completely dissolved, adding lactic acid to adjust the pH value to 4.5, and stirring to prepare gel, thus obtaining the plant prebiotics gel which can balance vaginal microbial flora and increase vaginal muscle elasticity.

Example 7

s1, weighing 60g of water, 20g of glycerol, 40715 g of poloxamer, 0.1g of osthole, 2g of garlic extract, 1g of prebiotics nutrient, 1g of zedoary turmeric oil, 3g of cyrtomium rhizome extract, 3g of Sinkiang arnebia root extract, 0.1g of melaleuca alternifolia leaf oil, 0.1g of rosemary leaf oil, 1g of hairyvein agrimonia herb extract, 0.1g of lavender oil, 0.1g of matrine, 0.06g of chlorhexidine gluconate and 0.1g of lactic acid for later use; wherein the prebiotic nutrient is galactooligosaccharide.

S4, sequentially adding osthole, garlic extract, prebiotics nutrient solution, zedoary turmeric oil, cyrtomium rhizome extract, Sinkiang arnebia root extract, melaleuca alternifolia leaf oil, rosemary leaf oil, hairyvein agrimonia herb extract, lavender oil, matrine and chlorhexidine gluconate into the mixed solution under stirring, stirring until the components are completely dissolved, adding lactic acid to adjust the pH value, and stirring to prepare gel, thus obtaining the plant prebiotics gel for balancing vaginal microbial flora and increasing vaginal muscle elasticity.

Example 8

s1, weighing 80g of water, 10g of glycerol, 40720 g of poloxamer, 0.5g of osthole, 5g of garlic extract, 5g of prebiotics nutrient, 8g of zedoary turmeric oil, 6g of cyrtomium rhizome extract, 10g of Sinkiang arnebia root extract, 1g of melaleuca alternifolia leaf oil, 1g of rosemary leaf oil, 5g of hairyvein agrimonia herb extract, 2g of lavender oil, 0.5g of matrine, 0.3g of chlorhexidine gluconate and 0.5g of lactic acid for later use; wherein the prebiotic nutrient is isomaltooligosaccharide.

S2, adding the weighed water into the pot, heating to 95 ℃, preserving heat for 35 minutes, and then cooling to room temperature.

Experimental example:

the plant prebiotic gel prepared in example 3 was tested for its bacteriostatic activity against different strains according to the standard GB15979-2002, and the test results are shown in table 1.

TABLE 1

As can be seen from the table 1, after the plant prebiotic gel prepared by the embodiment of the invention acts on bacteria (staphylococcus aureus and escherichia coli) for 2min, the bacteriostasis rate can reach more than 97 percent, and after the plant prebiotic gel acts for 5min, the bacteriostasis rate exceeds 99.99 percent; the bacteriostasis rate of the compound preparation after 5min of action on candida albicans exceeds 99.99 percent; the influence on beneficial bacteria lactobacillus crispatus in the vagina is small, and the bacteriostasis rate is lower than 10% after 24 hours of action. Therefore, the plant prebiotic gel can not only rapidly inhibit the growth of staphylococcus aureus and escherichia coli, but also has strong inhibition effect on the growth of fungi (candida albicans); but the inhibition of the growth of vaginal probiotics is weak. The plant prebiotic gel prepared by the embodiment of the invention can quickly and efficiently inhibit the growth of harmful bacteria, reduce the influence of the antibacterial agent on the growth of beneficial bacteria and effectively maintain the balance of vaginal flora.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The plant prebiotics gel is characterized by comprising the following components in parts by weight: 60-80 parts of water, 10-20 parts of glycerol, 40715-20 parts of poloxamer, 0.1-0.5 part of osthole, 2-5 parts of garlic extract, 1-5 parts of prebiotics nutrient, 1-8 parts of zedoary turmeric oil, 3-6 parts of cyrtomium rhizome extract, 3-10 parts of sinkiang arnebia root extract, 0.1-1 part of alternanthera leaf oil, 0.1-1 part of rosemary leaf oil, 1-5 parts of hairyvein agrimonia herb extract, 0.1-2 parts of lavender oil, 0.1-0.5 part of matrine, 0.06-0.3 part of chlorhexidine gluconate and 0.1-0.5 part of lactic acid.

2. The plant prebiotic gel according to claim 1, which comprises the following components in parts by weight: 65-68 parts of water, 12-15 parts of glycerol, 40716-18 parts of poloxamer, 0.2-0.3 part of osthole, 2.5-3 parts of garlic extract, 4-4.5 parts of prebiotics nutrient, 1.5-2 parts of zedoary turmeric oil, 3.2-4 parts of cyrtomium rhizome extract, 5.1-6 parts of Sinkiang arnebia root extract, 0.4-0.5 part of alternanthera leaf oil, 0.25-0.5 part of rosemary leaf oil, 2-3 parts of hairyvein agrimonia herb extract, 0.5-1 part of lavender oil, 0.3-0.4 part of matrine, 0.25-0.3 part of chlorhexidine gluconate and 0.25-0.3 part of lactic acid.

3. A plant prebiotic gel according to claim 1 or 2, wherein the prebiotic nutrient is at least one of inulin, α -glucan oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide.

4. A method of preparing a plant prebiotic gel as described in any of claims 1 to 3 comprising the steps of:

5. The method for preparing a plant prebiotic gel according to claim 4, wherein in the step, the water is cooked by the following method: and heating the water to 90-95 ℃, preserving the heat for 30-35 minutes, and then cooling to room temperature.

6. The method for preparing a plant prebiotic gel according to claim 4, wherein in the step, the temperature of the mixture of the boiled water, poloxamer 407 and glycerol is 8-12 ℃.

7. The method for preparing a plant prebiotic gel according to claim 4, wherein the pH of the plant prebiotic gel is = 4-4.5.

8. A plant prebiotic gel made by the method of any one of claims 4 to 7.

9. Use of a plant prebiotic gel according to any one of claims 1 to 3, 8 in the manufacture of a formulation for balancing vaginal microbial flora and/or increasing vaginal muscle tone.