CN112322451A - Annual production system for large-scale culture of plankton such as microalgae - Google Patents

Annual production system for large-scale culture of plankton such as microalgae Download PDF

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CN112322451A
CN112322451A CN202011333740.9A CN202011333740A CN112322451A CN 112322451 A CN112322451 A CN 112322451A CN 202011333740 A CN202011333740 A CN 202011333740A CN 112322451 A CN112322451 A CN 112322451A
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microalgae
plankton
pool
facility
annual production
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王智刚
赵昕
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/18—Open ponds; Greenhouse type or underground installations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

Abstract

The invention provides an annual production system for culturing plankton such as microalgae and the like on a large scale. Aiming at the defects of various natural resources caused by huge market demands, the problems that the continuous culture is difficult to realize, the input-output ratio is not ideal, the highest efficiency cannot be reached, the management is difficult, the seasonality is strong, the large-scale annual production cannot be realized and the like due to the past plankton such as microalgae and the like are solved, the invention provides an annual production system for culturing the plankton such as the microalgae and the like on a large scale, the special and high-efficiency culture system is arranged in the facility, so that the land resource is saved, the biomass born by the unit area is huge, and the facility is not influenced by the weather, because of adopting unique design, provides the optimal condition for the growth of the plankton such as the microalgae and the like, improves the efficiency of photosynthesis, realizes the annual continuous high-yield cultivation of the plankton such as the microalgae and the like, and the management is easy, the popularization is easy, and the requirements of the market and the breeding industry are more extensive.

Description

Annual production system for large-scale culture of plankton such as microalgae
Technical Field
The invention relates to the technical field of facility agriculture, modern agriculture and high-efficiency agriculture, in particular to an annual production system for culturing plankton such as microalgae on a large scale.
Technical Field
With the development of society and the pursuit of high-quality life by human beings, high-quality aquatic resources are obtained in nature, the huge demands of the market can not be met for a long time, in addition, due to industrial and agricultural pollution, ship leakage, radioactive nuclear wastewater sea-entering events and the like, the potential safety hazard exists in aquatic foods, the modes of culturing various fresh water and seawater aquatic products in facilities have existed for many years, the breeding technology of various aquatic products is gradually mastered by human beings, most of artificially bred aquatic larvae are lack of planktons as opening baits due to the influence of seasonal temperature when being cultured out of season, such as unicellular algae plants (chrysophyceae, chlorella, chaetoceros, diatom, haematococcus, spirulina and the like), bacteria, and zooplanktons (rotifer, brine shrimp, lobster trionyx) with the characteristics of breeding under certain conditions, Chironomid larvae, cladocera, copepods and the like) are used as the bait for aquatic larvae in different development stages, and as plankton is rapid in propagation and growth, rich in nutrition and good in palatability, many scientists at home and abroad research the artificial culture of the bait, but the research has not made a great breakthrough all the time. At present, artificial culture of plankton only needs to be put into some fields and artificial facilities, only some yield is improved within a certain period, and the plankton still can be influenced by seasonal temperature. When large-scale aquaculture is carried out in facilities, such as oyster, shellfish, snail, clam, corbicula and mussel for filter feeding, required plankton baits such as microalgae and the like cannot be met, so far, no ideal substitute baits exist, and a part of bottlenecks encountered by aquaculture in the facilities are in the link of bait shortage, so that the annual production target is difficult to realize. At present, microalgae are used as baits in aquaculture industry, and in other fields such as: the extraction of the biological fuel oil, the fatty acid and the pigment is also widely applied to health-care food, cosmetics, pharmaceutical industry and the like. The cultivation of the microalgae not only needs proper temperature, but also needs certain illumination conditions for photosynthesis, the previous cultivation of the microalgae is completed in a transparent container, when the value is increased in need, the microalgae subjected to multistage cultivation is inoculated into a transparent pipeline reactor for closed cultivation, or is subjected to standing open cultivation in a shallow pool and open cultivation for circular stirring in a runway pool, and the cultivation also belongs to eating by the day, the day and night temperature change fluctuation of the mode is large, the pH value is unstable, the open cultivation is easy to infect other microorganisms to influence the purity, the loss is caused, and the traditional cultivation mode has small water body, low biomass productivity and difficulty in achieving ideal production effect.
Disclosure of Invention
In order to solve the problems that the conventional culture method of plankton such as microalgae is greatly influenced by weather, cannot realize continuous culture, is easy to pollute, has low productivity and the like, the invention provides an annual production system for culturing plankton such as microalgae on a large scale, which comprises the following steps: a cylindrical deep pool which is deep underground and accords with the fluid mechanics principle is arranged in a positive pressure factory building facility with a good closed condition, underwater plant light sources are arranged in the pool from top to bottom in a multi-dimensional distribution mode, and the inner wall of the pool is integrally designed in a light reflecting mode to prevent light energy loss; the bottom of the water tank is provided with a stirring paddle, and an aeration device is arranged at a proper position as required; a necessary water inlet and outlet pipeline system and a temperature control device are arranged at a proper position of the water pool; the pool is provided with a floating foam removing device, and a rail hoisting device capable of translating is arranged above the pool and is used for necessary maintenance and management in the pool; the operation of the whole system is completed by an intelligent program control system, and the system instructs each execution unit to complete each operation through the data returned by various sensors after analysis and processing; the system is provided with emergency power supply equipment for the use from time to time; the harvesting operation can be scientifically finished in a dilution and filtration mode every day according to the biomass density, and the rest is continuously subjected to value-added culture, so that the continuous production of planktons such as microalgae and the like is realized.
Drawings
FIG. 1 is a schematic diagram of an annual production system for large-scale cultivation of plankton such as microalgae according to the present invention:
1 is a positive pressure facility 2, a cylindrical deep pool water pool 3 and a program control system
4 is a plant lamp light source 5 and an aeration device 6
7 is a floating foam removing device 8, a water inlet and outlet pipeline system 9 is a temperature control device
10 the hoisting equipment 11 is emergency power supply equipment with various sensors 12
13 blower with biological filter
Detailed Description
1. The invention provides an annual production system for large-scale culture of plankton such as microalgae and the like, which comprises the following steps: a cylindrical deep pool water pool 2 which is deep into the underground and has firm structure and accords with the fluid mechanics principle is arranged in a positive pressure facility 1 (a factory building, a greenhouse and the like) capable of resisting and blocking severe weather, and underwater plant light sources 4 are arranged in the pool from top to bottom in a multi-dimensional distribution mode, so that the purpose of arranging the light sources is to create uninterrupted photosynthesis conditions for microalgae plants in a water body and enable the microalgae plants to be added with value quickly; the inner wall of the water tank is integrally designed in a light reflecting mode, so that light energy loss is prevented; the bottom of the water pool is provided with a stirring paddle 5, a plurality of aeration devices 6 are arranged at proper positions as required, gas lift power generated in the bubble floating process and the stirring paddle act simultaneously to stir the water body, so that gas exchange in water is promoted, and carbon dioxide gas fertilizer required by algae photosynthesis is sufficient; the water body which flows in a rotating way is relieved, so that planktons such as microalgae and the like can be suspended and uniformly distributed, and the precipitation and wall attachment of the planktons are prevented; the pool is provided with a floating foam removing device 7 which can automatically remove the foam floating on the water surface; a necessary water inlet and outlet pipeline system 8 and a temperature control device 9 are arranged at a proper position of the water pool; arranging a rail hoisting device 10 capable of translating above a pool in the facility, wherein the rail hoisting device is used for necessary maintenance and management in the pool; the system is provided with various sensors 11 which are matched, an intelligent program control system 3 dynamically monitors physicochemical indexes such as temperature, illumination, flow rate, biological density, salinity, PH value, change of nutrient components and the like, and implements dynamic regulation and control to keep elements such as temperature, nutrition, light, gas and the like in an optimal state all the time, and due to high metabolic efficiency, photosynthetic accumulation is promoted, and the purpose of biomass rapid proliferation is achieved; to avoid losses due to blackouts, the system is provided with emergency power supply equipment 12.
2. The positive pressure factory building facilities use the fan 13 with the biological filter to supply air uninterruptedly and provide clean sterile air, so that the air pressure inside the factory building is always higher than that outdoors to form a positive pressure environment, the pollution probability in the biological culture process is reduced, and the system operation is operated according to the standard of the sterile workshop regulations.
3. Before the facility is put into use, the inside of the facility and a water tank need to be sterilized and disinfected in an effective mode, then an intelligent program control system is started to enable a positive pressure system and various sensors to enter working states, water is injected into the water tank (disinfection reaches the standard), after various physicochemical indexes reach the standard, a target organism is inoculated for value-added culture, according to the growth requirements of the cultured microalgae, a nutrition formula and growth condition indexes with the optimal proportion are input into the system, the program automatically carries out adjustment according to the data sent back by the sensors, and various execution units (such as a temperature control unit, a light control unit, an aeration unit, a trace element supply control unit, a water supply and drainage unit, a hoisting system control unit and the like) are instructed to complete various; by applying the system, single pure products can be cultured in a large scale according to application requirements, and various plankton and the like can be simultaneously mixed and cultured to be used as aquatic feed.
Effects of the invention
By applying the invention, the influence of weather is avoided, the water surface utilization rate is high, the three-dimensional cultivation of the plankton such as the microalgae is realized, the land resource is saved, the variation fluctuation of the water temperature and the water quality of a large water body is extremely small, the optimal condition is provided for the growth of the plankton such as the microalgae, the photosynthesis efficiency is improved, and the sanitary and safe standard-reaching product can be continuously produced; the positive pressure design of the facility achieves the standard of an ultra-clean workshop, reduces the probability of pollution in the biological culture process, has low labor cost, simple management and easy popularization, and really realizes the large-scale annual high-yield cultivation of plankton such as microalgae and the like.

Claims (4)

1. An annual production system for large-scale cultivation of plankton such as microalgae is characterized in that: a cylindrical deep pool with an underwater light source is arranged in the positive pressure facility, and the inner wall of the pool adopts a reflecting design; the bottom of the water tank is provided with a stirring paddle; an aeration device, necessary water inlet and outlet pipelines and temperature control equipment are arranged at a proper position of the water tank; the intelligent program control system instructs each execution unit to complete each operation through data returned by various sensors; the system is provided with emergency power supply equipment.
2. The system according to claim 1, wherein the system is used for the mass cultivation of plankton such as microalgae, and comprises: the cylindrical deep pool is arranged in a factory facility with positive pressure, an underwater light source is arranged in the pool, and the inner wall of the pool adopts a light reflecting design.
3. The system according to claim 1 or 2, which is used for the annual production of large-scale cultivation of plankton such as microalgae, and is characterized in that: the positive pressure plant facility uses the fan with the biological filter to continuously supply air, provides clean sterile air, ensures that the air pressure inside the facility is always higher than that outdoors to form a positive pressure environment, and can reduce the pollution probability in the biological culture process.
4. The system according to claim 1, wherein the system is used for the mass cultivation of plankton such as microalgae, and comprises: the intelligent program control system inputs nutrition formula and growth condition index with optimal proportion into the system according to the growth requirement of plankton such as cultivated microalgae, and the necessary operations are automatically completed by each execution unit instructed by the program through analysis and processing according to the data returned by the sensor.
CN202011333740.9A 2020-11-24 2020-11-24 Annual production system for large-scale culture of plankton such as microalgae Pending CN112322451A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011333740.9A CN112322451A (en) 2020-11-24 2020-11-24 Annual production system for large-scale culture of plankton such as microalgae

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011333740.9A CN112322451A (en) 2020-11-24 2020-11-24 Annual production system for large-scale culture of plankton such as microalgae

Publications (1)

Publication Number Publication Date
CN112322451A true CN112322451A (en) 2021-02-05

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