CN112304910A - Fluorescence immunochromatography test strip detection device and method - Google Patents
Fluorescence immunochromatography test strip detection device and method Download PDFInfo
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- CN112304910A CN112304910A CN202011073241.0A CN202011073241A CN112304910A CN 112304910 A CN112304910 A CN 112304910A CN 202011073241 A CN202011073241 A CN 202011073241A CN 112304910 A CN112304910 A CN 112304910A
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- 238000012360 testing method Methods 0.000 title claims abstract description 44
- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000003317 immunochromatography Methods 0.000 title claims description 4
- 230000005284 excitation Effects 0.000 claims abstract description 26
- 238000001228 spectrum Methods 0.000 claims abstract description 23
- 239000000835 fiber Substances 0.000 claims abstract description 12
- 239000013307 optical fiber Substances 0.000 claims description 7
- 239000010453 quartz Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 238000003556 assay Methods 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 239000012611 container material Substances 0.000 claims description 2
- 238000004020 luminiscence type Methods 0.000 claims description 2
- 238000002310 reflectometry Methods 0.000 claims description 2
- 230000003595 spectral effect Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 description 7
- 238000001917 fluorescence detection Methods 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- Immunology (AREA)
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- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a fluorescence immunochromatographic test strip detection device and a method. Detection device includes stand support, lift group, integrating sphere, excitation light source, optic fibre, power, spectrum appearance and computer, the stand support sets up on the horizontal plane, lift group cover is established on the stand support, lift group is equipped with and connects the mirror body hole, the integrating sphere is fixed in and connects the mirror body downthehole, be equipped with income light mouth, light-emitting window, sample mouth on the integrating sphere respectively, go into light mouth, light-emitting window and set up perpendicularly on the integrating sphere, the excitation light source is gone into the light mouth through optic fibre and integrating sphere and is connected, the power is connected with the excitation light source, the spectrum appearance input is connected with the integrating sphere light-emitting window through optic fibre, and the spectrum appearance power is connected with the spectrum appearance, the computer is connected with the spectrum appearance output through the USB interface. The invention is suitable for various fluorescent immunochromatographic test strips, is convenient for the detection of a sample to be detected, and improves the detection quality of the detected sample.
Description
Technical Field
The invention relates to the technical field of immunodiagnosis instruments, in particular to a device and a method for quantitatively detecting a fluorescence immunochromatographic test strip.
Background
The immunochromatography technology is a rapid immunoassay technology combining the chromatography technology with the immunolabeling technology, and has the advantages of simple operation, wide dynamic detection range, high sensitivity and the like. And during detection, sample detection information is obtained by taking an image or color of the collected sample after detection.
The fluorescence immunoassay technology combines the high sensitivity of the fluorescence technology with the high specificity of the immunology technology, and the current fluorescence immunochromatographic test strip detection device is widely used for detecting serum antibodies, bacteria and viruses. Due to different molecular structures of substances, the wavelength of ultraviolet light which can be absorbed and the wavelength of fluorescence which can be emitted are also different, and the substance to be detected can be determined qualitatively by utilizing the characteristic. Under a certain condition, the higher the concentration of a substance to be detected is, the stronger the fluorescence emitted after ultraviolet irradiation is; conversely, the lower the concentration, the weaker the emitted fluorescence; thereby allowing quantitative analysis of the substance to be measured. Each fluorescent substance has an excitation waveband capable of being selectively absorbed and a specific emission waveband, but the existing detection device of the fluorescence immunochromatographic test strip only has one fluorescence light path and can only detect a detection card marked by one fluorescent substance.
Disclosure of Invention
The invention aims to provide a fluorescence immunochromatographic test strip detection device and a fluorescence immunochromatographic test strip detection method, which can conveniently and rapidly carry out quantitative detection on fluorescence samples to be detected with different wavelengths, are simple and convenient to operate and wide in detection range, eliminate light interference in the environment and are beneficial to improving the result accuracy and the working efficiency.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a fluorescence immunochromatographic test strip detection device, includes: stand support, lift group, integrating sphere, excitation light source, optic fibre, power, spectrum appearance power, computer, the stand support sets up on the horizontal plane, lift group cover is established on the stand support, there is the mirror body hole on the lift group, the integrating sphere is fixed in and connects the mirror body downthehole, be equipped with income light mouth, light-emitting window, sample mouth on the integrating sphere respectively, go into light mouth, light-emitting window and set up on the integrating sphere perpendicularly, the excitation light source is gone into the light mouth through optic fibre and integrating sphere and is connected, the power is connected with the excitation light source, the spectrum appearance input is connected with the integrating sphere light-emitting window through optic fibre, and the spectrum appearance power is connected with the spectrum appearance, the computer is connected with the spectrum appearance output through the USB interface for receive the spectral data of output and handle and assay.
Optionally, the lifting group is provided with a focusing knob for moving and adjusting the height of the integrating sphere, and the adjusting speed is high, so that the operation is convenient.
Optionally, the integrating sphere is made of a PTFE inner container material, has a wide spectrum range covering ultraviolet-visible-near infrared (200-.
Optionally, the sample port is arranged at the upper part of the integrating sphere, the diameter of the sample port is 6-10 mm, and a clamping groove and a shading cover are arranged on the sample port.
Optionally, the clamping groove is a concave area formed by a quartz plate and a PVC plate as a frame, a test strip placement area is arranged in the concave area and used for placing the immunochromatographic test strip after complete reaction, and a quartz pressing plate is arranged on the concave area and used for limiting the placement depth of the test strip.
Optionally, the light shielding cover is a black light-tight casing for shielding an external light source.
Optionally, the excitation light source is an LED light source group with a replaceable wavelength range, and is used for detecting fluorescent materials with different wavelength bands.
A method for testing the luminescence of a fluorescent material by a fluorescence immunochromatographic test strip detection device is characterized in that: the method comprises the following specific steps:
1) and a power supply of the excitation light source is turned on, the excitation light source uses 395-400nm LED lamps, the excitation light source is connected with the light inlet of the integrating sphere through an optical fiber, the light outlet of the integrating sphere is connected with the spectrometer through an optical fiber, and the spectrometer is connected with a USB interface of the computer through a data line.
2) The fluorescence immunochromatographic test strip is reversely placed in a clamping groove at the upper end of the integrating sphere and is covered with a light shielding cover, and fluorescence excited by an excitation light source in the integrating sphere is coupled to the spectrometer through an optical fiber.
3) And acquiring and storing the fluorescence signal intensity measured by the spectrometer through Ocean view software arranged on a computer.
The method of the invention has the following advantages:
(1) the fluorescence immunochromatographic analyzer provided by the invention can conveniently and simply replace the wavelength of the excitation light source, and does not need to use professional and complex installation tools and professional technicians.
(2) The method realizes the detection of the fluorescent substances with different wavelengths by replacing the excitation light sources with different wavelength ranges.
(3) According to the method, the fixed clamping groove is arranged as the immunochromatographic test strip placing area, and the test strip restraint structure is arranged in the test strip placing area, so that the test strip is positioned more accurately, errors caused by position changes of a test zone of the test strip can be reduced, and the test precision is improved.
Drawings
In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings used in the description of the present invention or the prior art will be briefly described below.
FIG. 1 is a schematic diagram of a fluorescence immunochromatographic test strip detection device of the present invention.
Fig. 2 is an enlarged schematic view of the lifting group of fig. 1.
Fig. 3 is an enlarged schematic view of the integrating sphere of fig. 1.
FIG. 4 is a schematic view of a test strip placement area of the present invention.
FIG. 5 is a flow chart of the quantitative detection method of the immunochromatographic test strip of the present invention.
The labels in the figure are: 1-power supply, 2-excitation light source, 3-upright post bracket, 4-integrating sphere, 5-shading cover, 6-spectrometer, 7-spectrometer power supply and 8-computer.
Detailed Description
In order that the objects, technical solutions and advantages of the present invention will become more apparent, the present invention will be further described in detail with reference to the accompanying drawings in conjunction with the following specific embodiments.
Referring to FIG. 1, one embodiment of the fluorescence detection device of the present invention comprises: stand support 3, lift group, integrating sphere 4, excitation light source 2, optic fibre, power 1, spectrum appearance 6, spectrum appearance power 7 and computer 8, stand support 3 sets up on the horizontal plane, lift group's cover is established on stand support 3, there is the lens hole on the lift group, integrating sphere 4 is fixed in and connects the lens downthehole, be equipped with income light mouth, light-emitting outlet, sample mouth on integrating sphere 4 respectively, go into light mouth, light-emitting outlet and set up perpendicularly on integrating sphere 4, excitation light source 2 is connected with integrating sphere 4 income light mouth through optic fibre, power 1 is connected with excitation light source 2, 6 input ends of spectrum appearance are connected with 4 light-emitting outlets of integrating sphere through optic fibre, and spectrum appearance power 7 is connected with spectrum appearance 6, computer 8 is connected with spectrum appearance 6.
It can be seen from the above embodiments that the fluorescence detection apparatus provided by the present invention, by arranging the excitation light source 2, the spectrometer 6 and the processing unit of the computer 8, performs quantitative detection on the fluorescence intensity on the test strip, and has the advantages of simple structure and convenient operation.
Referring to fig. 2, the lifting group is provided with a focusing knob for moving and adjusting the height of the integrating sphere 4.
Referring to fig. 3, the integrating sphere 4 is provided with a light inlet, a light outlet and a sample port, the diameter of the integrating sphere 4 is 6-10 mm, the integrating sphere 4 is made of a PTFE inner container, the inner container has higher reflectivity and a more delicate inner surface structure, and the more stable material is not yellowed and deteriorated due to oxidation reaction with air.
It can be seen from the above embodiments that the integrating sphere 4 provided by the present invention collects the fluorescence excited by the excitation light source 2 through the light inlet, the fluorescence is reflected inside the integrating sphere 4, the reflected fluorescence is coupled to the spectrometer 6 through the light outlet via the optical fiber, and the data is processed and analyzed by the computer 8 to obtain the information of the sample to be measured.
Referring to fig. 4, the present invention provides an embodiment of the test strip placement area, which includes: the test strip is characterized in that the concave area is formed by taking a quartz piece and a PVC plate as a frame, a quartz pressing piece is arranged on the concave area and used for limiting the placing depth of the test strip, and the black lightproof shading cover 5 is arranged above the test strip placing area and used for shading an external light source.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the idea of the invention, also technical features in the above embodiments or in different embodiments may be combined and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity. Therefore, any omissions, modifications, substitutions, improvements and the like that may be made without departing from the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (8)
1. A fluorescence immunochromatographic test strip detection device is characterized by comprising: stand support, lift group, integrating sphere, excitation light source, optic fibre, power, spectrum appearance power and computer, the stand support sets up on the horizontal plane, lift group cover is established on the stand support, it connects the mirror body hole to be equipped with on the lift group, the integrating sphere is fixed in and connects the mirror body downthehole, be equipped with income light mouth, light-emitting window, sample mouth on the integrating sphere respectively, go into light mouth, light-emitting window and set up on the integrating sphere perpendicularly, the excitation light source is gone into the light mouth through optic fibre and integrating sphere and is connected, the power is connected with the excitation light source, the spectrum appearance input is connected with the integrating sphere light-emitting window through optic fibre, and the spectrum appearance power is connected with the spectrum appearance, the computer is connected with the spectrum appearance output through the USB interface for receive the spectral data of output and handle and assay.
2. The fluorescence immunochromatographic test strip detection device of claim 1, characterized in that: the lifting group is provided with a focusing knob used for moving and adjusting the height of the integrating sphere.
3. The fluorescence immunochromatographic test strip detection device of claim 1, characterized in that: the integrating sphere is made of a PTFE inner container material, has a wide spectrum range covering ultraviolet-visible-near infrared, can provide ultrahigh reflectivity in a 200-plus 2500nm waveband, and has a more exquisite internal surface structure.
4. The fluorescence immunochromatographic test strip detection device of claim 1, characterized in that: the diameter of the sample port is 6-10 mm, and a clamping groove and a shading cover are arranged on the sample port.
5. The fluorescence immunochromatographic test strip detection device of claim 4, characterized in that: the clamping groove is a concave area formed by taking a quartz plate and a PVC plate as a frame, a test strip placing area is arranged in the concave area and used for placing the immunochromatographic test strip after complete reaction, and a quartz pressing plate is arranged on the concave area and used for limiting the placing depth of the test strip.
6. The fluorescence immunochromatographic test strip detection device of claim 4, characterized in that: the light shield cover is a black, light-tight housing.
7. The fluorescence immunochromatographic test strip detection device of claim 1, characterized in that: the excitation light source is an LED light source group with a replaceable wavelength range.
8. A method for testing the luminescence of a fluorescent material by a fluorescence immunochromatographic test strip detection device is characterized in that: the method comprises the following specific steps:
1) the power supply of an excitation light source is turned on, the excitation light source uses a 395-400nm visible light source, the excitation light source is connected with an integrating sphere light inlet through an optical fiber, an integrating sphere light outlet is connected with a spectrometer through an optical fiber, and the spectrometer is connected with a USB interface of a computer through a data line;
2) the fluorescence immunochromatography test strip is reversely placed in a clamping groove at the upper end of the integrating sphere and is covered with a shading cover, and fluorescence inside the integrating sphere is coupled to the spectrometer through an optical fiber;
3) and acquiring and storing the fluorescence signal intensity measured by the spectrometer through Ocean view software arranged on a computer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011073241.0A CN112304910A (en) | 2020-10-09 | 2020-10-09 | Fluorescence immunochromatography test strip detection device and method |
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| CN202011073241.0A CN112304910A (en) | 2020-10-09 | 2020-10-09 | Fluorescence immunochromatography test strip detection device and method |
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| JP2018155676A (en) * | 2017-03-21 | 2018-10-04 | 国立研究開発法人物質・材料研究機構 | Integrating sphere with sample holding part, and optical measurement method and quantum efficiency measurement method using the same |
| CN111413328A (en) * | 2020-04-28 | 2020-07-14 | 上海泰辉生物科技有限公司 | Dual-mode instant detection system |
-
2020
- 2020-10-09 CN CN202011073241.0A patent/CN112304910A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005106410A1 (en) * | 2004-04-30 | 2005-11-10 | Arkray, Inc. | Point light source and optical device comprising same |
| CN101514962A (en) * | 2009-03-31 | 2009-08-26 | 东北电力大学 | Fluorescent mark immunity test strip detector |
| CN102359817A (en) * | 2011-03-08 | 2012-02-22 | 中国科学院福建物质结构研究所 | System for testing yield of up-conversion luminescence absolute quantum |
| CN103105380A (en) * | 2011-11-10 | 2013-05-15 | 李建国 | Time resolution fluorescence system |
| CN104931472A (en) * | 2015-06-10 | 2015-09-23 | 东北电力大学 | Continuous detector for multi-wavelength excited fluorescence labeling immune test strips |
| CN105403548A (en) * | 2015-12-08 | 2016-03-16 | 厦门稀土材料研究所 | Temperature variable spectral measurement device |
| JP2018155676A (en) * | 2017-03-21 | 2018-10-04 | 国立研究開発法人物質・材料研究機構 | Integrating sphere with sample holding part, and optical measurement method and quantum efficiency measurement method using the same |
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Application publication date: 20210202 |