CN112292962A - Composition for pelletization of seed of Chinese pine - Google Patents

Composition for pelletization of seed of Chinese pine Download PDF

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CN112292962A
CN112292962A CN202011184719.7A CN202011184719A CN112292962A CN 112292962 A CN112292962 A CN 112292962A CN 202011184719 A CN202011184719 A CN 202011184719A CN 112292962 A CN112292962 A CN 112292962A
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seeds
pelleted
adhesive
formula
filler
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高捍东
高燕
薛晓明
李娟�
俞元春
于凤强
陆嘉程
陈学琦
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Nanjing Forestry University
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Nanjing Forestry University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06—Coating or dressing seed

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Abstract

The Chinese pine seed pelleting composition is formed by compounding a filler and an adhesive, wherein the mass concentration of the adhesive is 0.5-1.25%, the balance of the filler is the mixture of clay, talcum powder and diatomite in any proportion, and the adhesive is sodium carboxymethylcellulose. The water-retaining agent can fully absorb water when meeting a small amount of rainfall to form a water storage balloon and then gradually release water; the adhesive has good cohesiveness and water solubility, and is the key for seed pelleting success and failure; meanwhile, the inert filler and the adhesive determine the hardness and the disintegration of the pelletized seeds and influence on the germination and emergence of the seeds. Therefore, the problems of disintegration, nutrition, water supply and the like of the pelleted pinus tabulaeformis seeds are solved, the survival rate of the seeds is improved, the growth of seedlings is promoted, the stress resistance of the seedlings is enhanced, a theoretical basis is provided for the pelleted technical research and application of the pinus tabulaeformis seeds, and a theoretical basis is laid for the pelleted technical research and application of the forest grass seeds.

Description

Composition for pelletization of seed of Chinese pine
Technical Field
The invention relates to the technical field of seed pelleting, in particular to a composition for seed pelleting of Chinese pine (Pinus tabuliformis Carr).
Background
Seed pelleting is one of the seed treatments, and mainly refers to a treatment for coating some substances on the surface of seeds to change the shape of the seeds, increase the volume of the seeds, promote the germination and growth of the plants, and improve the resistance of the seeds. The volume of the pelleted seed can be increased several times, called pelleted seed or seed pellet. They are not only suitable for mechanical precision sowing, but also improve the quality of seeds and minimize the pollution of chemical substances to the environment.
At present, the research of the forest grass seed pelleting technology has achieved abundant achievements in processing mechanization, seeding quantity quantification and the like, but the seed pelleting aspect still has some problems to be solved urgently: firstly, the weight of the seeds is increased by adopting a pelleting technology, but the contradiction between breakage and germination is not solved; secondly, the pelleting components are relatively single, and the survival rate of seeds cannot be guaranteed due to drought and rain shortage in mountainous areas and desert areas and lack of nutritional ingredients required by plants; thirdly, in the area with water deficiency, the content of the water retention agent added into the pelleted seeds is the main problem of the pelleted formula, and is also the key point of whether the pelleted seeds can emerge and the emergence rate is high or low.
Disclosure of Invention
The technical problem to be solved is as follows: the invention provides a composition for pelleting Chinese pine seeds, thereby solving the problems of disintegration, nutrition, water supply and the like of the pelleted Chinese pine seeds, improving the survival rate of the seeds, promoting the growth of seedlings and enhancing the stress resistance of the seedlings.
The technical scheme is as follows: the Chinese pine seed pelletizing composition is compounded with stuffing of 0.5-1.25 wt% and adhesive of sodium carboxymethyl cellulose as the rest, and the stuffing is mixture of clay, talcum powder and diatomite in any proportion.
Preferably, the adhesive is sodium carboxymethylcellulose with the mass concentration of 1.25%, and the balance of filler, wherein the filler is a mixture of clay, talcum powder and diatomite according to the mass ratio of 1:8: 1.
Preferably, the above-mentioned pinus tabulaeformis seed pelleting composition further contains not more than 7wt.% of a water-retaining agent, which is a hamming water-retaining agent (gel/granule-56743) or boya water-retaining agent (farmyard manure 2008 word 3412).
Preferably, the content of the hamming water retention agent is 7wt.%, the content of the adhesive is 0.5 wt.% of sodium carboxymethyl cellulose, and the balance is a filling material, wherein the filling material is a mixture of clay, talcum powder and diatomite according to the mass ratio of 1:8: 1.
Preferably, the boya water retention agent content is 7wt.%, the adhesive is 1.0 wt.% of sodium carboxymethyl cellulose, and the balance is a filler, wherein the filler is a mixture of clay, talcum powder and diatomite in a mass ratio of 3:4: 3.
Has the advantages that: according to the invention, through the screening of the pill coating formula of the Chinese pine seeds and the research of the germination and seedling growth characteristics of the Chinese pine seeds with different pill formulas under drought stress, the optimal pill formula of the Chinese pine seeds is determined, the strong and weak drought tolerance of the germination and seedling growth of the Chinese pine seeds with different pill formulas is explored, and the optimal pill formula is further screened, wherein the formula comprises a water-retaining agent, an adhesive and an inert filler. The water-retaining agent can fully absorb water when meeting a small amount of rainfall to form a water storage balloon and then gradually release water; the adhesive has good cohesiveness and water solubility, and is the key for seed pelleting success and failure; meanwhile, the inert filler and the adhesive determine the hardness and the disintegration of the pelletized seeds and influence on the germination and emergence of the seeds. Therefore, the problems of disintegration, nutrition, water supply and the like of the pelleted pinus tabulaeformis seeds are solved, the survival rate of the seeds is improved, the growth of seedlings is promoted, the stress resistance of the seedlings is enhanced, a theoretical basis is provided for the pelleted technical research and application of the pinus tabulaeformis seeds, and a theoretical basis is laid for the pelleted technical research and application of the forest grass seeds.
Drawings
FIG. 1 is a graph of the effect of drought stress on the rate of emergence of pinus tabulaeformis seeds of different pelleting formulations;
it can be seen that the 4 pelleted formulations and Control (CK) pinus tabulaeformis seed emergence rates generally decreased with increasing stress intensity. Under W0, the M4 pelleting formula has the best effect on the emergence of the pine seeds; at W1, emergence rate was lower for N12 pellet formulation alone than control; the M4 pelleted formulation gave the best results for emergence of pinus tabulaeformis seeds at W2.
FIG. 2 is a graph showing the effect of drought stress on the seedling rate of Pinus tabulaeformis seeds of different pelleting formulations;
it can be seen that the seedling rate of the pinus tabulaeformis seeds of the Control (CK) and the 4 pelleting formulas is in a descending trend along with the increase of the stress intensity. The M4 pelleted formulation had the greatest number of seedlings under W0, W1 and W2. Under W0 and W1, the seedling rate of the pill formula of M16, N10 and N12 is lower than that of a Control (CK); under W2, the seedling rate of the Chinese pine seeds of other formulas except the pill formulas of M4 and N10 is lower than that of the Control (CK).
FIG. 3 is a graph of the effect of drought stress on the seedling height of pinus tabulaeformis seedlings of different pelleting formulations;
it can be seen that the seedling height of the Control (CK) and 4 pelletized formulas of Chinese pine shows a descending trend with the increasing strength of drought stress. The M4 pelleted formula pinus tabulaeformis seeds all grew better than Control (CK) at W0, W1 and W2. Under W0 and W2, the heights of seedlings of M16, N10 and N12 pelleted formula pinus tabulaeformis seeds are all lower than that of a Control (CK); at W1, the heights of the seedlings of the pinus tabulaeformis seeds were higher than the Control (CK) except for the M16 pelleted formulation.
FIG. 4 is a graph of the effect of drought stress on the average dry weight of pinus tabulaeformis seedlings for different pelleting formulations;
it can be seen that the average dry weight of the Control (CK) and 4 pelleted formula pinus tabulaeformis seedlings generally decreased with increasing stress intensity. The average dry weight of M4 pelleted formulated pinus tabulaeformis seedlings was maximal at W0; at W1, only M16 pelleted formula pinus tabulaeformis seedlings had a lower average dry weight than the control; the average dry weight of M4, M16 pelleted formulated pinus tabulaeformis seedlings was higher than the control under W2.
FIG. 5 is a graph of the effect of drought stress on chlorophyll content of pinus tabulaeformis seedlings of different pelleting formulations;
it can be seen that the chlorophyll content of the seedlings of the Control (CK) and 4 pelletized formulas showed a decreasing trend with increasing stress intensity. At W0 and W1, only N12 pellets of formula pinus tabulaeformis seedlings had a lower chlorophyll content than the Control (CK); at W2, the chlorophyll content of the N12 pelleted formula Chinese pine seedling was higher than that of the Control (CK), indicating that chlorophyll decomposition or synthesis of the Control (CK) and the remaining pelleted formula was more severely inhibited than that of the N12 pelleted formula under this stress.
FIG. 6 is a graph of the effect of drought stress on the soluble protein content in the conifer leaves of different pelletized pinus tabulaeformis seed seedlings;
as can be seen, with the increasing stress intensity, the content of soluble protein in the conifer leaves of the 4 pelletized pinus tabulaeformis seedlings is in an increasing trend, and the content of the soluble protein in the conifer leaves of the 4 pelletized pinus tabulaeformis seedlings is in a trend of firstly decreasing and then increasing. Under W0, the content of soluble protein in the needle leaves of 4 kinds of pill-formed formula Chinese pine seedlings is lower than that of a Control (CK); under W1 and W2, the content of soluble protein in the needle leaves of the formula pinus tabulaeformis seedlings pelleted by M4, M16, N10 and N12 is higher than that of a Control (CK); the 4 pelleting formulas can promote piny leaves of Chinese pine seedlings to synthesize some highly hydrophilic new proteins so as to enhance the hydration of the proteins and play a role in resisting dehydration under drought stress.
FIG. 7 is a graph of the effect of drought stress on the content of proline in coniferous leaves of seedlings of pinus tabulaeformis seeds of different pelleting formulations;
it can be seen that with the increasing stress intensity, the proline content in the conifer leaves of the M4, M16 and N12 pelleted formula and the Control (CK) Chinese pine seedling tends to increase, and the proline content in the conifer leaves of the N10 pelleted formula tends to decrease first and then increase. Under W0, the proline content in the coniferous leaves of 4 kinds of pill-formed formula Chinese pine seedlings is higher than that of a Control (CK); at W1, pellet formula N10 only was lower than Control (CK); under W2, the contents of proline in the coniferous leaves of M4 and M10 pelleted formula Chinese pine seedlings are higher than that of a Control (CK).
FIG. 8 is a graph of the effect of drought stress on the MDA content in the conifer leaves of pinus tabulaeformis seeds of different pelleting formulations;
it can be seen that the MDA content of 4 pelleted formulations and the Control (CK) pinus tabulaeformis seedlings increased with increasing stress intensity. Under W0 and W1, the malondialdehyde content in the needle leaves of the pelleted formula Chinese pine seedlings of M4, M16, N10 and N12 is lower than that of a Control (CK); under W2, the malonaldehyde content in the conifer of M4 and M12 pelleted formula Chinese pine seedlings is lower than that of the Control (CK), and the malonaldehyde content in the conifer of the rest pelleted formula Chinese pine seedlings is higher than that of the Control (CK)
FIG. 9 shows the effect of drought stress on SOD activity in coniferous leaves of seedlings of pinus tabulaeformis seeds of different pelleting formulations;
it can be seen that, as the stress intensity is increased, the SOD activity in the leaves of the pinus tabulaeformis seedlings of the Control (CK) and the 4 pelleted formulas is complex, the N10 pelleted formula and the Control (CK) have the trend of descending first and then ascending, the M4 and N12 pelleted formulas have the trend of ascending first and then descending, and the M16 pelleted formula has the trend of ascending.
FIG. 10 is a graph of the effect of drought stress on POD activity in conifer leaves of different pelleted formulations of Pinus tabulaeformis seeds;
it can be seen that the POD activity in the needle leaf of the M4, N10, N12 pelleted formula and the Control (CK) Chinese pine seedling increased with the increase of the stress intensity, and that the POD activity in the needle leaf of the M16 pelleted formula increased first and then decreased. POD activity was higher in conifer needles of M16 pelleted formula pinus tabulaeformis seedlings only than Control (CK) at W0; under W1, POD activity in the conifer of M16 and N10 pelleted formula Chinese pine seedlings is higher than that of a Control (CK); under W2, POD activity in the needle leaves of the pelleted formula Chinese pine seedlings of M4, M16, N10 and N12 is higher than that of a Control (CK).
FIG. 11 is a graph of the effect of drought stress on CAT activity in conifer leaves of seedlings of pinus tabulaeformis seeds of different pelleting formulations.
It can be seen that as the stress intensity increases, the CAT activity in the needle leaves of the M4, M16 and N10 pelleted formula and the Control (CK) Chinese pine seedling increases, and the CAT activity in the needle leaves of the N12 pelleted formula increases. At W0, CAT activity was slightly higher in the needle leaves of only M4-pelleted formula seedlings than Control (CK); under W1, the CAT activity in the needle leaf of the formula Chinese pine seedlings pelleted by N10 and N12 is higher than that of the Control (CK), and under W2, the CAT activity in the needle leaf of the formula Chinese pine seedlings pelleted by M4, M16, N10 and N12 is higher than that of the Control (CK).
Detailed Description
The following examples further illustrate the present invention but are not to be construed as limiting the invention. Modifications and substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit and substance of the invention.
Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
Example 1:
results and analysis of first stage adhesive and Filler screening
The adhesive types selected for this test in the attached tables 1-4: sodium carboxymethylcellulose (a); the filler (B) is selected from clay, talcum powder and diatomite. The type of the water retention agent (C) is Haiming high-efficiency drought-resistant water retention agent and Boya agriculture and forestry water retention agent.
The mass concentration of the sodium carboxymethylcellulose (A) is respectively set to be 0.5 percent (A1), 0.75 percent (A2), 1.0 percent (A3), 1.25 percent (A4) and 4 concentration gradients; the filling material (B) is prepared from clay, talcum powder and diatomite in different proportions. Two schemes are obtained by completely random design in the test. Each scheme further adopts L16(45) The orthogonal test design method is used for pelleting seeds and exploring an optimal pelleting formula. A total of 32 formulation combinations.
The results of the granulation and molding of the Chinese pine seeds with different pelleting formulas are shown in tables 5 and 6. It can be seen that the different pelleting formulas in the scheme I and the scheme II can granulate and form the Chinese pine seeds. The agglomeration phenomenon of the Chinese pine seed in different pelleting formulas in the scheme I is more serious than that in the scheme II. The pelleting formulas of M7, M8 and M13 in the scheme I cause serious granulation and agglomeration of the Chinese pine seeds, the appearance of the pelleted seeds is poor, and the similar phenomenon does not occur in the scheme II. The pelleting formulas of M1, M6 and M11 in the scheme I and N1, N6 and N1 in the scheme II can ensure that the Chinese pine seeds are pelletized and formed without agglomeration, and the pelleted seeds have good appearance and have the potential of becoming an excellent formula.
The results of comparing the quality indexes of the seed pelletization of the Chinese pine by different pelletization formulas are shown in tables 7 and 8. It can be seen that the seed yield and the single seed yield of the Chinese pine seeds with different pelleting formulas in the scheme I and the scheme II are both high. The maximum pressure which can be borne by the Chinese pine seeds with different pelleting formulas in the scheme I and the scheme II is 25-40N. The cracking rates of the Chinese pine seeds in different pelleting formulas in the scheme I are different, wherein the mean values of the cracking rates of the Chinese pine seeds in the pelleting formulas M1, M4, M6, M7, M13, M15 and M16 reach 100%, and the cracking rates of the Chinese pine seeds in the pelleting formulas M5, M11, M12 and M14 are obviously different. In the scheme II, the cracking rates of the Chinese pine seeds in different pelleting formulas are different, and the mean value of the cracking rates of the Chinese pine seeds in the rest pelleting formulas except for the N11, N13, N14 and N15 pelleting formulas reaches 100 percent.
And secondly, carrying out tests on the influence of different pelleted seed formulas on the seed vigor, wherein the tests comprise tests on the germination rate, the germination vigor, the germination index and the seed vigor index.
With reference to tables 9-12, it can be seen that:
in the pill formula of the scheme I, the pill formulas with better comprehensive performance in indexes of germination rate, germination vigor, germination index and vitality index of the Chinese pine seeds are respectively M4 pill formulas, M16 pill formulas and M12 pill formulas, wherein the germination rate, the germination vigor, the germination index and the vitality index of the Chinese pine seeds in the M4 pill formula are highest. In the formula pelleted in the scheme II, the formula pelleted with better comprehensive performance in indexes of the germination rate, the germination vigor, the germination index and the vitality index of the Chinese pine seeds is respectively N16, N12 and N10, wherein the germination rate, the germination index and the vitality index of the Chinese pine seeds in the formula pelleted with N16 are all the highest, and the germination vigor of the Chinese pine seeds in the formula pelleted with N12 is the highest. Because the M12 pelleting formula has a caking phenomenon in the pelleting and coating process of the Chinese pine seeds, and the M16 pelleting formula is the same as the N16 pelleting formula, the finally selected optimal formula is the M4, M16, N12 and N10 pelleting formula.
The germination rates of the selected 4 pelletized formula Chinese pine seeds are lower than that of a Control (CK), wherein the germination rate of the M4 pelletized formula Chinese pine seeds is reduced by 4.2% compared with that of the Control (CK), and generally, the germination rate of the pelletized formula seeds is reduced by about 7% compared with that of the Control (CK), which is a normal phenomenon, but the germination vigor, the germination index and the vitality index of the M4 pelletized formula Chinese pine seeds are all improved compared with that of the Control (CK), so that the M4 pelletized formula has a certain promotion effect on the germination of the Chinese pine seeds. The germination rate, the germination vigor, the germination index and the vitality index of the M16, N12 and N10 pelleted formula Chinese pine seeds are lower than those of a Control (CK), and probably because the pelleted seed surface layer of the M16, N12 and N10 pelleted formula Chinese pine seeds forms a 'hydration film' in a standard germination test, the respiration of the seeds is influenced, and the vitality of the seeds is reduced.
Study on seed germination and seedling growth characteristics of Chinese pine with different pelleting formulas under drought stress in second stage
First, 4 kinds of preferred formula pelleted pine seeds (M4, M16, N10, N12) selected in the first stage and naked pine seeds (CK) without any treatment were used as experimental materials.
Respectively sowing M4, M16, N10, N12 pinus tabulaeformis pelleted seeds and pinus tabulaeformis naked seeds (CK) in pots with the diameter of 16cm and the height of 8cm, wherein the pot culture medium is under-forest brown loam, and a small amount of sand, perlite and peat are uniformly mixed, and the volume weight of the soil is 0.92g.cm-3Porosity 78.00%, PH 7.09. The test was carried out in a climatic chamber with 12h of light daily, 25 ℃ daytime temperature, 20 ℃ night temperature and 65.00% humidity. Each formula (M4, M16, N10, N12 and CK) is set at 3 levels in a pine seed drought stress test, 1 pot of seeds is sowed at each level, 80 seeds are sowed in each pot, and the steps are repeated for 3 times. The horizontal settings are as follows:
w0: is suitable for moisture. The water content of the soil is 60 percent of the field water capacity of the soil (the water content of the soil is 34.20 percent)
W1: moderate drought. The water content of the soil is 40 percent of the field water capacity of the soil (the water content of the soil is 22.80 percent)
W2: severe drought. The water content of the soil is 20 percent of the field water capacity of the soil (the water content of the soil is 11.40 percent)
The water gradient basins are sealed by transparent plastic bags (small air holes are arranged on the plastic bags) to reduce water evaporation. The electronic scale is used for weighing soil and the basin weight when the seeding is started, watering is carried out according to the design requirement of the soil water content, and 17 is carried out every day later: 00 an electronic scale is used for weighing the soil basin, and water is supplemented to the design requirement according to the change of the soil basin. Recording the time of emergence, observing the emergence rate (exposing soil as standard) after 7d, observing once a day, recording the number of seedlings, counting the number of plants forming seedlings after 30d, measuring the height and biomass of the seedlings, and measuring various physiological and biochemical indexes by adopting Chinese pine leaves. The measurement results are shown in the attached figures 1-11.
Secondly, correlation analysis is carried out on various indexes measured by the Chinese pine seeds and the seedlings of the Chinese pine seeds with different pelleting formulas under drought stress, the results are shown in a table 13, and it can be seen that most indexes of the drought stress do not reach significant correlation levels, which indicates that the drought stress indexes are independent.
In order to accurately and quickly screen an optimal pelleting formula, the research carries out principal component analysis on each measured index,
and then, comprehensively evaluating the drought stress resistance of the Chinese pine seeds with different pelleting formulas by combining a membership function method, mainly carrying out quantitative conversion by adopting a fuzzy mathematical membership function formula, and then averaging the membership function values of all indexes to carry out comparison among different pelleting formulas. With reference to table 14, it can be seen that the drought stress tolerance of the pinus tabulaeformis seeds according to different pelleting formulas from large to small: m4 pellet formulation > N10 pellet formulation > M16 pellet formulation > Control (CK) > N12 pellet formulation.
The third stage selects the optimal formula
The study further screened the optimum pinus tabulaeformis seed pelleting formula as M4 formula through the preliminary screening and simulated drought test of the pelleting formula, namely, the adhesive concentration is 0.5%, the filling material proportion comprises 10% of clay, 80% of talcum powder, 10% of diatomite and 7% of hamming water retention agent, and then the N10 pelleting formula (the adhesive concentration is 1.0%, the filling material proportion comprises 30% of clay, 40% of talcum powder, 30% of diatomite and 7% of boya water retention agent) and the M16 pelleting formula (the adhesive concentration is 1.25%, the filling material proportion comprises 10% of clay, 80% of talcum powder, 10% of diatomite and 0% of hamming water retention agent).
TABLE 1
Figure BDA0002749944110000061
TABLE 2
Figure BDA0002749944110000071
TABLE 3
Figure BDA0002749944110000072
TABLE 4
Figure BDA0002749944110000073
TABLE 5
Figure BDA0002749944110000074
Figure BDA0002749944110000081
TABLE 6
Figure BDA0002749944110000082
Figure BDA0002749944110000091
TABLE 7
Treatment combination Pelleting formula Seed ratio/% Single seed rate/%) Single grain stress/N Cracking rate/%
A1B1C1 M1 100.00 98.00 32.68 100.00Aa
A1B2C2 M2 96.00 94.00 30.77 93.33Aab
A1B3C3 M3 94.00 90.00 36.17 97.78Aa
A1B4C4 M4 99.00 98.00 30.42 100.00Aa
A2B1C2 M5 96.00 94.00 25.28 48.89Cd
A2B2C1 M6 100.00 98.00 32.84 100.00Aa
A2B3C4 M7 92.00 90.00 37.51 100.00Aa
A2B4C3 M8 94.00 92.00 34.62 93.33Aab
A3B1C3 M9 96.00 94.00 30.59 91.11Aab
A3B2C4 M10 94.00 92.00 30.97 91.11Aab
A3B3C1 M11 98.00 96.00 34.74 51.11Cd
A3B4C2 M12 92.00 90.00 32.66 73.33Bc
A4B1C4 M13 92.00 90.00 34.98 100.00Aa
A4B2C3 M14 94.00 92.00 32.36 82.22ABbc
A4B3C2 M15 92.00 90.00 37.35 100.00Aa
A4B4C1 M16 100.00 98.00 31.84 100.00Aa
TABLE 8
Figure BDA0002749944110000092
Figure BDA0002749944110000101
TABLE 9
Treatment combination Formula I for pelleting Germination rate/% Formula II for pelleting Germination rate/%
A1B1C1 M1 29 N1 29
A1B2C2 M2 33 N2 34
A1B3C3 M3 23 N3 8
A1B4C4 M4 68 N4 33
A2B1C2 M5 18 N5 26
A2B2C1 M6 19 N6 19
A2B3C4 M7 28 N7 10
A2B4C3 M8 17 N8 18
A3B1C3 M9 8 N9 20
A3B2C4 M10 19 N10 41
A3B3C1 M11 18 N11 18
A3B4C2 M12 38 N12 51
A4B1C4 M13 19 N13 35
A4B2C3 M14 10 N14 17
A4B3C2 M15 27 N15 30
A4B4C1 M16 52 N16 52
CK 71
Watch 10
Figure BDA0002749944110000102
Figure BDA0002749944110000111
TABLE 11
Figure BDA0002749944110000112
Figure BDA0002749944110000121
TABLE 12
Treatment combination Formula I for pelleting Vitality index Formula II for pelleting Vitality index
A1B1C1 M1 0.066 N1 0.066
A1B2C2 M2 0.071 N2 0.078
A1B3C3 M3 0.046 N3 0.012
A1B4C4 M4 0.176 N4 0.070
A2B1C2 M5 0.032 N5 0.050
A2B2C1 M6 0.030 N6 0.030
A2B3C4 M7 0.052 N7 0.015
A2B4C3 M8 0.035 N8 0.026
A3B1C3 M9 0.016 N9 0.033
A3B2C4 M10 0.047 N10 0.087
A3B3C1 M11 0.037 N11 0.037
A3B4C2 M12 0.092 N12 0.105
A4B1C4 M13 0.041 N13 0.074
A4B2C3 M14 0.014 N14 0.035
A4B3C2 M15 0.057 N15 0.060
A4B4C1 M16 0.139 N16 0.139
CK 0.172
Watch 13
Figure BDA0002749944110000122
Note: p < 0.05, P < 0.01;
t1: the rate of emergence; t2: the seedling rate; t3: the height of the seedlings is high; t4 mean dry weight of seedlings; t5: chlorophyll content; t6 soluble protein; t7: (ii) proline content; t8 malondialdehyde content; t9: SOD activity; t10: (ii) a POD activity; t11: CAT activity; the lower left corner is the correlation coefficient among all indexes under moderate drought; the upper right corner is the correlation coefficient among all indexes under severe drought.
TABLE 14
Figure BDA0002749944110000131

Claims (5)

1. The Chinese pine seed pelleting composition is characterized by being formed by compounding a filler and an adhesive, wherein the mass concentration of the adhesive is 0.5-1.25%, the balance of the filler is the mixture of clay, talcum powder and diatomite in any proportion, and the adhesive is sodium carboxymethylcellulose.
2. The composition of claim 1, wherein the adhesive is sodium carboxymethylcellulose with a mass concentration of 1.25%, and the rest is filler, and the filler is a mixture of clay, talcum powder and diatomite in a mass ratio of 1:8: 1.
3. The pinus tabulaeformis seed pelleted composition according to claim 1, characterized by further containing not more than 7wt.% of a water retaining agent, which is a hamming water retaining agent (gel/granule-56743) or boya water retaining agent (farmyard manure 2008 word No. 3412).
4. The composition of claim 3, wherein the hamming water retention agent content is 7wt.%, the adhesive is 0.5 wt.% sodium carboxymethyl cellulose, and the rest is a filler, wherein the filler is a mixture of clay, talcum powder and diatomite in a mass ratio of 1:8: 1.
5. The composition of claim 3, wherein the boya water retention agent is 7wt.%, the adhesive is 1.0 wt.% sodium carboxymethyl cellulose, and the rest is a filler, wherein the filler is a mixture of clay, talcum powder and diatomite in a mass ratio of 3:4: 3.
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