CN112284863A - Application method of Reishi-Giemsa dye liquor - Google Patents
Application method of Reishi-Giemsa dye liquor Download PDFInfo
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- CN112284863A CN112284863A CN202011131516.1A CN202011131516A CN112284863A CN 112284863 A CN112284863 A CN 112284863A CN 202011131516 A CN202011131516 A CN 202011131516A CN 112284863 A CN112284863 A CN 112284863A
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- dyeing
- vaginal secretion
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 210000003756 cervix mucus Anatomy 0.000 claims abstract description 80
- 238000004043 dyeing Methods 0.000 claims abstract description 75
- 239000011521 glass Substances 0.000 claims abstract description 52
- 238000002156 mixing Methods 0.000 claims abstract description 15
- 239000011248 coating agent Substances 0.000 claims abstract description 12
- 238000000576 coating method Methods 0.000 claims abstract description 12
- 239000012192 staining solution Substances 0.000 claims description 25
- 239000008213 purified water Substances 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 238000004140 cleaning Methods 0.000 claims description 12
- 206010046901 vaginal discharge Diseases 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 11
- 239000007788 liquid Substances 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 6
- 241000894006 Bacteria Species 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 241000233866 Fungi Species 0.000 abstract description 3
- 238000004040 coloring Methods 0.000 abstract description 3
- 239000010419 fine particle Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 25
- 238000010186 staining Methods 0.000 description 22
- 238000007711 solidification Methods 0.000 description 6
- 230000008023 solidification Effects 0.000 description 6
- 238000002738 Giemsa staining Methods 0.000 description 3
- 238000007447 staining method Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000007664 blowing Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a method for using a dyeing liquid of Rayleigh-Giemsa, which comprises the steps of firstly uniformly mixing the dyeing liquid A with the dyeing liquid B, then uniformly coating vaginal secretion on a glass slide, and solidifying the vaginal secretion on the glass slide; the vaginal secretion is dyed, and then visible components in the vaginal secretion are dyed, the dyeing mode has proper cell coloring depth, is beneficial to observation and judgment, has lighter background color and is basically colorless, forms of various cells and microorganisms are clearly presented, misjudgment on the visible components can not be caused, especially, the dyeing effect of fine particles such as fungi, bacteria and the like is clear and visible, and meanwhile, the dyeing time is greatly shortened.
Description
Technical Field
The invention relates to the technical field of medical reagents, in particular to a method for using a dyeing liquid of Rayleigh-Giemsa.
Background
The examination of vaginal secretion is one of the routine items in clinical examinations of modern medicine such as gynecology and obstetrics, and plays an important role in the diagnosis of diseases. For example, the detection and analysis system for the visible components of vaginal secretion can improve the detection rate of causative factors by using a staining method. In the traditional Rui's-Jimsa staining method, after staining for 2min by staining solution A, staining solution B is added, and after air blowing and uniform mixing, staining is carried out for 2min, and two-step staining is carried out, but the staining method has poor staining effect on vaginal secretion and long staining time.
Disclosure of Invention
The invention aims to provide a method for using a dyeing liquid of Rayleigh-Giemsa, and aims to solve the technical problems that the dyeing method in the prior art has poor dyeing effect on vaginal secretion and long dyeing time.
In order to achieve the purpose, the method for using the dyeing liquor of the giemsa rapieri comprises the following steps:
mixing the staining solution A and the staining solution B according to a quantitative ratio to obtain a mixed dye;
coating vaginal secretion on a glass slide uniformly, and solidifying the vaginal secretion on the glass slide;
and (4) dripping the mixed dye on solidified vaginal secretion for dyeing, and then cleaning.
Wherein, in the step of coating the vaginal secretion on the glass slide evenly and solidifying the vaginal secretion on the glass slide:
the curing operation is as follows: and (3) putting the glass slide coated with the vaginal secretion into an oven, and drying at 40-60 ℃.
Wherein, in the step of dripping the mixed dye on solidified vaginal secretion for dyeing, and then cleaning:
before the mixed dye is dripped on the solidified vaginal secretion for dyeing, the glass slide needs to be inclined by 20-40 degrees.
Wherein, in the step of dripping the mixed dye on solidified vaginal secretion for dyeing, and then cleaning:
and (4) dripping the mixed dye on the solidified vaginal secretion for dyeing, wherein the dyeing time is 20-40 s.
Wherein, in the step of dripping the mixed dye on solidified vaginal secretion for dyeing, and then cleaning:
the stained vaginal discharge was washed with purified water.
Wherein, after the mixed dye is dripped on solidified vaginal secretion for dyeing, the cleaning step is carried out:
and drying the cleaned vaginal secretion, and then performing microscopic examination.
The invention has the beneficial effects that: uniformly mixing the staining solution A and the staining solution B, then uniformly coating vaginal secretion on a glass slide, and solidifying the vaginal secretion on the glass slide; the vaginal secretion is dyed, and then visible components in the vaginal secretion are dyed, the dyeing mode has proper cell coloring depth, is beneficial to observation and judgment, has lighter background color and is basically colorless, forms of various cells and microorganisms are clearly presented, misjudgment on the visible components can not be caused, especially, the dyeing effect of fine particles such as fungi, bacteria and the like is clear and visible, and meanwhile, the dyeing time is greatly shortened.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of the steps of example 1 of the present invention.
Fig. 2 is a flowchart of the steps of embodiment 2 of the present invention.
Fig. 3 is a flowchart of the steps of embodiment 3 of the present invention.
FIG. 4 is a graph showing the first staining effect after staining vaginal discharge in the prior art.
FIG. 5 is a graph showing the effect of a second staining procedure after staining vaginal secretions according to the prior art.
FIG. 6 is a third graph showing the effect of staining vaginal secretions in the prior art.
FIG. 7 is a graph showing the first staining effect of the vaginal discharge of the present invention after staining.
FIG. 8 is a graph showing the effect of a second staining after staining vaginal secretions in accordance with the present invention.
FIG. 9 is a graph showing a third staining effect of the vaginal discharge of the present invention after staining.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Example 1, referring to fig. 1, the present invention provides a method for using a giemsa-giemsa dye liquor, comprising the steps of:
s1: mixing the staining solution A and the staining solution B according to a quantitative ratio to obtain a mixed dye;
s2: coating vaginal secretion on a glass slide uniformly, putting the glass slide coated with the vaginal secretion into an oven, and baking at 40 ℃ to finish solidification;
s3: setting the glass slide at an inclination angle of 20 degrees, then dripping the mixed dye on the solidified vaginal secretion for dyeing for 20s, and then cleaning by using purified water;
s4: and drying the cleaned vaginal secretion, and then performing microscopic examination.
The dyeing solution A is a Ruhrstan's dyeing solution, the dyeing solution B is a Jimsan dyeing solution, and in the step of mixing the dyeing solution A and the dyeing solution B according to a quantitative ratio to obtain a mixed dye, the dyeing solution A and the dyeing solution B are stirred and mixed in an environment at 30-34 ℃; in the step of washing the stained vaginal discharge with purified water: and sucking the purified water by using a pipette, and then squeezing the pipette to slowly drip the purified water from the upper end of the glass slide. The application method of the dyeing liquid of the Rayleigh-Giemsa comprises the following steps: stirring and mixing the staining solution A and the staining solution B in a quantitative ratio in an environment of 30 ℃ to obtain a mixed dye; then coating the vaginal secretion on a glass slide uniformly, putting the glass slide coated with the vaginal secretion into an oven, and baking at 40 ℃ to finish solidification; and then, the glass slide is inclined by 20 degrees, the mixed dye is dripped on the solidified vaginal secretion for dyeing, the dyeing time is 20s, then, the glass slide is cleaned by purified water, the purified water is sucked by a suction tube and then is slowly dripped from the upper end of the glass slide by squeezing the suction tube, the glass slide is inclined by 20 degrees, the back surface of the glass slide is prevented from being polluted by the dye, the process of cleaning the back surface of the glass slide can be reduced, and the time is saved. The application method of the Reishi-Giemsa staining solution is applied to staining of vaginal secretion, can stain visible components of the vaginal secretion, clearly shows the forms of various cells and microorganisms, and greatly shortens the staining time.
Example 2, referring to fig. 2, the present invention provides a method for using giemsa, comprising the following steps:
s1: mixing the staining solution A and the staining solution B according to a quantitative ratio to obtain a mixed dye;
s2: coating vaginal secretion on a glass slide uniformly, putting the glass slide coated with the vaginal secretion into an oven, and baking at 60 ℃ to finish solidification;
s3: the glass slide is inclined by 40 degrees, then the mixed dye is dripped on the solidified vaginal secretion for dyeing, the dyeing time is 40s, and then the glass slide is cleaned by purified water;
s4: and drying the cleaned vaginal secretion, and then performing microscopic examination.
The dyeing solution A is a Ruhrstan's dyeing solution, the dyeing solution B is a Jimsan dyeing solution, and in the step of mixing the dyeing solution A and the dyeing solution B according to a quantitative ratio to obtain a mixed dye, the dyeing solution A and the dyeing solution B are stirred and mixed in an environment at 30-34 ℃; in the step of washing the stained vaginal discharge with purified water: and sucking the purified water by using a pipette, and then squeezing the pipette to slowly drip the purified water from the upper end of the glass slide. The application method of the dyeing liquid of the Rayleigh-Giemsa comprises the following steps: stirring and mixing the staining solution A and the staining solution B in a quantitative ratio in an environment of 34 ℃ to obtain a mixed dye; then coating the vaginal secretion on a glass slide uniformly, putting the glass slide coated with the vaginal secretion into an oven, and baking at 60 ℃ to finish solidification; and then, inclining the glass slide by 40 degrees, dripping the mixed dye on the solidified vaginal secretion for dyeing for 40s, and then cleaning by using purified water, wherein the purified water is sucked by a suction tube, then the suction tube is squeezed, the purified water is slowly dripped from the upper end of the glass slide, the inclination of the glass slide is 40 degrees, the back surface of the glass slide can be prevented from being polluted by the dye, the process of cleaning the back surface of the glass slide can be reduced, and the time is saved. The application method of the Reishi-Giemsa staining solution is applied to staining of vaginal secretion, can stain visible components of the vaginal secretion, clearly shows the forms of various cells and microorganisms, and greatly shortens the staining time.
Example 3, referring to fig. 3, the present invention provides a method for using giemsa, comprising the following steps:
s1: mixing the staining solution A and the staining solution B according to a quantitative ratio to obtain a mixed dye;
s2: coating vaginal secretion on a glass slide uniformly, putting the glass slide coated with the vaginal secretion into an oven, and baking at 50 ℃ to finish solidification;
s3: the glass slide is inclined by 30 degrees, then the mixed dye is dripped on the solidified vaginal secretion for dyeing for 30s, and then the glass slide is cleaned by purified water;
s4: and drying the cleaned vaginal secretion, and then performing microscopic examination.
The dyeing solution A is a Ruhrstan's dyeing solution, the dyeing solution B is a Jimsan dyeing solution, and in the step of mixing the dyeing solution A and the dyeing solution B according to a quantitative ratio to obtain a mixed dye, the dyeing solution A and the dyeing solution B are stirred and mixed in an environment at 30-34 ℃; in the step of washing the stained vaginal discharge with purified water: and sucking the purified water by using a pipette, and then squeezing the pipette to slowly drip the purified water from the upper end of the glass slide. The application method of the dyeing liquid of the Rayleigh-Giemsa comprises the following steps: stirring and mixing the staining solution A and the staining solution B in a quantitative ratio in an environment with the temperature of 32 ℃ to obtain a mixed dye; then coating the vaginal secretion on a glass slide uniformly, putting the glass slide coated with the vaginal secretion into an oven, and baking at 50 ℃ to finish solidification; and then, inclining the glass slide by 30 degrees, dripping the mixed dye on the solidified vaginal secretion for dyeing for 30s, and then cleaning by using purified water, wherein the purified water is sucked by a suction tube, then the suction tube is squeezed, the purified water is slowly dripped from the upper end of the glass slide, the inclination of the glass slide is 40 degrees, the back of the glass slide can be prevented from being polluted by the dye, the process of cleaning the back of the glass slide can be reduced, and the time is saved. The application method of the Reishi-Giemsa staining solution is applied to staining of vaginal secretion, can stain visible components of the vaginal secretion, clearly shows the forms of various cells and microorganisms, and greatly shortens the staining time.
Please refer to fig. 4 to fig. 9, to summarize the following: uniformly mixing the staining solution A and the staining solution B, then uniformly coating vaginal secretion on a glass slide, and solidifying the vaginal secretion on the glass slide; and then the vaginal secretion is dyed, and further visible components in the vaginal secretion are dyed, the existing dyeing method has over heavy bottom color, and bacteria and the bottom color cannot be distinguished. The background color is easily misjudged as bacteria. The dyeing method of the invention has appropriate cell coloring depth, is beneficial to observation and judgment, has lighter background color which is basically colorless, and has clear shapes of various cells and microorganisms without causing misjudgment on visible components, particularly has clear and visible dyeing effect on fine particles such as fungi, bacteria and the like, and greatly shortens the dyeing time.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. The method for using the dyeing liquor of the Rayleigh-Giemsa is characterized by comprising the following steps of:
mixing the staining solution A and the staining solution B according to a quantitative ratio to obtain a mixed dye;
coating vaginal secretion on a glass slide uniformly, and solidifying the vaginal secretion on the glass slide;
and (4) dripping the mixed dye on solidified vaginal secretion for dyeing, and then cleaning.
2. The method of using the swill-giemsa stain of claim 1, wherein in the step of spreading vaginal secretions onto the slide and solidifying the vaginal secretions on the slide:
the curing operation is as follows: and (3) putting the glass slide coated with the vaginal secretion into an oven, and drying at 40-60 ℃.
3. The method of using the dyeing liquor of giemsa rapi according to claim 1, characterized in that in the step of dripping the mixed dye on the solidified vaginal secretions for dyeing, followed by rinsing:
before the mixed dye is dripped on the solidified vaginal secretion for dyeing, the glass slide needs to be inclined by 20-40 degrees.
4. The method of using the dyeing liquor of giemsa rapi according to claim 1, characterized in that in the step of dripping the mixed dye on the solidified vaginal secretions for dyeing, followed by rinsing:
and (4) dripping the mixed dye on the solidified vaginal secretion for dyeing, wherein the dyeing time is 20-40 s.
5. The method of using the dyeing liquor of giemsa rapi according to claim 1, characterized in that in the step of dripping the mixed dye on the solidified vaginal secretions for dyeing, followed by rinsing:
the stained vaginal discharge was washed with purified water.
6. The method of using the dyeing rapes-giemsa according to claim 5, characterized in that after the step of dripping the mixed dye on the solidified vaginal secretions for dyeing, followed by rinsing:
and drying the cleaned vaginal secretion, and then performing microscopic examination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011131516.1A CN112284863A (en) | 2020-10-21 | 2020-10-21 | Application method of Reishi-Giemsa dye liquor |
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| CN202011131516.1A CN112284863A (en) | 2020-10-21 | 2020-10-21 | Application method of Reishi-Giemsa dye liquor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113933130A (en) * | 2021-09-24 | 2022-01-14 | 中山大学附属第八医院（深圳福田） | Preparation method and application of human blastocyst protozoon staining specimen |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109799123A (en) * | 2018-12-29 | 2019-05-24 | 广州和能生物科技有限公司 | A kind of method of quick carry out Rui Shi Giemsa staining |
| CN109971212A (en) * | 2019-03-05 | 2019-07-05 | 桂林优利特医疗电子有限公司 | A kind of Rui Shi-Jim Sa compound stain solution and preparation method thereof |
| CN110631882A (en) * | 2019-10-22 | 2019-12-31 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
-
2020
- 2020-10-21 CN CN202011131516.1A patent/CN112284863A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109799123A (en) * | 2018-12-29 | 2019-05-24 | 广州和能生物科技有限公司 | A kind of method of quick carry out Rui Shi Giemsa staining |
| CN109971212A (en) * | 2019-03-05 | 2019-07-05 | 桂林优利特医疗电子有限公司 | A kind of Rui Shi-Jim Sa compound stain solution and preparation method thereof |
| CN110631882A (en) * | 2019-10-22 | 2019-12-31 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
Non-Patent Citations (1)
| Title |
|---|
| 周庭银 等主编: "《胃肠道感染实验诊断与临床诊治》", 上海科学技术出版社, pages: 191 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113933130A (en) * | 2021-09-24 | 2022-01-14 | 中山大学附属第八医院（深圳福田） | Preparation method and application of human blastocyst protozoon staining specimen |
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