CN105936873B - A kind of cleaning structure of hybridization instrument - Google Patents
A kind of cleaning structure of hybridization instrument Download PDFInfo
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- CN105936873B CN105936873B CN201610415365.XA CN201610415365A CN105936873B CN 105936873 B CN105936873 B CN 105936873B CN 201610415365 A CN201610415365 A CN 201610415365A CN 105936873 B CN105936873 B CN 105936873B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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Abstract
The invention discloses a kind of cleaning structures of hybridization instrument, including horizontal-extending Transfer pipe on pedestal and pedestal, the Transfer pipe includes feeding-passage, flushing channel, air blasting passage successively from front to back, cleaning head is respectively arranged on the right side of the left side of the flushing channel and air blasting passage, blowing head is provided on the left of the air blasting passage；The cleaning structure is directed to the need of work of hybridization instrument, pass through rational structural improvement, it will be by the hybridization of flow operations, cleaning, colour developing, drying operating procedure becomes streamlined operation, in bulk quantity of chips crossover operation, pass through streamlined operation, can constantly add in need to operate chip, after one section of reaction time, result can be gone out by persistently having operation to complete chip, save general reaction time during batch operation, in addition pass through cleaning head, chip groove spill imbibition position, the design of blowing head, make sample that can keep clean enough in the hybridization reaction course of work, ensure reaction effect.
Description
Technical field
The present invention relates to molecular hybridization fields, particularly a kind of cleaning structure applied to automatic Hybridization Oven.
Background technology
At present, general nucleic acid hybridization is all that nucleic acid hybridization behaviour is carried out by the way of manually imbibition and liquid feeding
Make, will be first placed in constant-temperature incubation device added with the chip of reaction sample and keep the temperature a period of time, cleaning process is used genetic chip
It is put into thermostat water bath and heat and vibrate mixing in a water bath, carry out the cleaning of nucleic acid hybridization in this way, it
Carry out color operation again afterwards.Using manual operations, liquid volume added levels of precision is difficult to ensure that, whole operation flow will spend more
Time, particularly with batch operation chip it is more when, need add in plurality of reagents, the heating water bath time is long, causes entirely to test
Time time is very long.For such case, someone just has developed automatic hybridizing instrument, although reducing manual operation difficulty,
Since each step operating time is not reduced, the W-response time is still longer.In addition, current existing automatic hybridizing instrument needle
It is also to be optimized to the automatic cleaning process of gene chip hybridization operation, it is susceptible to operating process and does not wash clean clearly and influence sample
Product reaction effect.
Invention content
For overcome the deficiencies in the prior art, the present invention provides a kind of cleaning structure of hybridization instrument.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of cleaning structure of hybridization instrument, including horizontal-extending Transfer pipe on pedestal and pedestal, the Transfer pipe
Include feeding-passage, flushing channel, air blasting passage, the left side of the flushing channel and the right side of air blasting passage successively from front to back
Side is respectively arranged with cleaning head, and blowing head is provided on the left of the air blasting passage.
As a preference, the front and rear sides of the Transfer pipe are respectively arranged with conveyer belt, divide on the conveyer belt
It is furnished with support salient point.
As a preference, the blowing head includes compressor, air knife lifting motor, air knife supporting rack and air knife and draws
Saddle, air knife lifting guide rail is provided in the air knife guide bracket, and air knife supporting rack lower end is provided with drying process aspirating needle
And air knife.
As a preference, the cleaning head includes water pump, cleaning nozzle lifting motor, cleaning nozzle supporting rack and cleaning
Mouth guide bracket is provided with cleaning nozzle guide rails in the cleaning nozzle guide bracket, and the cleaning nozzle supporting rack lower end is provided with imbibition
Needle, liquid feeding pin.
As a preference, heating plate is provided on the Transfer pipe.
As a preference, the chip groove for chip placement is provided on the Transfer pipe, the chip is recessed
The side edge of slot is provided with imbibition recess.
As a preference, the imbibition recess includes for the cleaning waste liquid suction port of cleaning head and for blowing
The drying waste liquid suction port of head.
As a preference, the air knife guide bracket includes the air knife translation on air knife level frame and air knife level frame
Motor.
The beneficial effects of the invention are as follows：The cleaning structure is directed to the need of work of hybridization instrument, by rational structural improvement,
It will become streamlined operation by the hybridization of flow operations, cleaning, colour developing, drying operating procedure, in bulk quantity of chips crossover operation,
By streamlined operation, can constantly add in need to operate chip, after one section of reaction time, persistently have operation to complete core
Piece can go out the general reaction time as a result, when saving batch operation, in addition by cleaning head, chip groove spill imbibition position,
The design of blowing head, makes sample that can keep clean enough in the hybridization reaction course of work, ensures reaction effect.
Description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the stereogram of the present invention；
Fig. 2 is the stereogram of blowing head in the present invention；
Fig. 3 is the stereogram of cleaning head in the present invention；
Fig. 4 is the partial enlarged view of chips groove part of the present invention.
Specific embodiment
It is with reference to the accompanying drawings and embodiments, right in order to which the object, technical solution and advantage for making the application are more clearly understood
The present invention is further elaborated.For the thorough explanation present invention, some certain details can be related in following description
Section.And in these specific details, of the invention then can may still realize, i.e., technical staff's use in fields is herein
These descriptions and the others skilled in the art that state into fields effectively introduce their work essence.In addition it needs
Bright, word " front side " used in the following description, " rear side ", " left side ", " right side ", " upside ", " downside " etc. refer to
Direction in attached drawing, word " interior " and " outer " are referred respectively to towards or away from the direction of particular elements geometric center, related skill
Art personnel above-mentioned direction is made it is simple, do not need to creative adjustment and should not be construed as skill other than the application protection domain
Art.It should be appreciated that specific embodiment described herein is only used to explain the application, it is not used to limit real protection model
It encloses.And it is the purpose of the present invention of avoiding confusion, due to well known manufacturing method, control program, part dimension, material composition, electricity
The technology of road layout etc. is not it will be understood that therefore they are described in detail.Reference Fig. 1~Fig. 4, a kind of hybridization instrument
Cleaning structure, including horizontal-extending Transfer pipe 2 on pedestal 1 and pedestal 1, the Transfer pipe 2 includes successively from front to back
There are feeding-passage 21, flushing channel 22, air blasting passage 23, the left side of the flushing channel 22 and the right side difference of air blasting passage 23
Cleaning head 3 is provided with, the left side of the air blasting passage 23 is provided with blowing head 4.
The front and rear sides of the Transfer pipe 2 are respectively arranged with conveyer belt 24, and it is convex that support is distributed on the conveyer belt 24
Point 25 is stablized convenient for the transmission of chip to be cleaned.Heating plate 5 is provided on the Transfer pipe 2.It is set on the Transfer pipe 2
The chip groove 6 for chip placement is equipped with, the side edge of the chip groove 6 is provided with imbibition recess 61, and the imbibition is recessed
Position 61 includes for the cleaning waste liquid suction port 62 of cleaning head 3 and the drying waste liquid suction port 63 for blowing head 4, wherein clearly
Waste liquid suction port when washing waste liquid suction port 62 as cleaning chip, waste liquid when finally being dried up as drying 63 chip of waste liquid suction port
Suction port
Other embodiment, with reference to the cleaning structure of a kind of hybridization instrument of Fig. 1, Fig. 2, Fig. 4, wherein referred to herein is " real
Apply example " refer to may be included in a particular feature, structure, or characteristic at least one realization method of the application.In the present specification not
" in the embodiment " occurred with place not refers both to same embodiment, nor individual or selective and other embodiment
Mutually exclusive embodiment.Embodiment includes horizontal-extending Transfer pipe 2 on pedestal 1 and pedestal 1, the Transfer pipe 2 from
Front to back includes feeding-passage 21, flushing channel 22, air blasting passage 23 successively, and the left side of the flushing channel 22 and blowing are logical
The right side in road 23 is respectively arranged with cleaning head 3, and the left side of the air blasting passage 23 is provided with blowing head 4.The blowing head 4 includes
There are compressor 41, air knife lifting motor 42, air knife supporting rack 43 and air knife guide bracket 44, the air knife lifting motor 42 is used to control
43 integral elevating of air knife supporting rack processed is provided with air knife lifting guide rail 45 in the air knife guide bracket.In the present embodiment, institute
It states air knife guide bracket 44 and includes air knife translation motor 48 on air knife level frame 47 and air knife level frame 47, air knife supporting rack 43
Lower end is provided with drying process aspirating needle 46 and air knife.Air knife is the application specific architecture with wind chamber, blowing seam.Compression is connected during blowing
The air knife valve of machine 41 is opened, and air knife starts to dry, and air knife translation motor 48 can control air knife to move left and right, control air knife from
Chip one end is moved to the other end.Liquid blots on 46 times general who has surrendered's chips of drying process aspirating needle on air knife supporting rack 43 simultaneously
Only, wherein drying waste liquid suction port 63 as waste liquid suction port, bottom are less than chip surface, drying process aspirating needle 46 declines
To the groove imbibition of waste liquid suction port 63.
Other embodiment, with reference to the cleaning structure of a kind of hybridization instrument of Fig. 1, Fig. 3, Fig. 4, including on pedestal 1 and pedestal 1
Horizontal-extending Transfer pipe 2, the Transfer pipe 2 include feeding-passage 21, flushing channel 22, blowing successively from front to back
Channel 23, the left side of the flushing channel 22 and the right side of air blasting passage 23 are respectively arranged with cleaning head 3, the air blasting passage 23
Left side be provided with blowing head 4.The cleaning head 3 includes water pump 31, cleaning nozzle lifting motor 32,33 and of cleaning nozzle supporting rack
Cleaning nozzle guide bracket 34 is provided with cleaning nozzle guide rails 35 in the cleaning nozzle guide bracket 34, for guiding cleaning nozzle guide bracket
34 integral elevatings.In the present embodiment, the vertically-mounted slide block type guide rail structure of cleaning nozzle guide rails 35, the cleaning nozzle branch
33 lower end of support is provided with aspirating needle, liquid feeding pin.Liquid feeding pin is suspended in liquid feeding above chip, and wherein liquid feeding position is in above chip about
0.5~1 centimeters, liquid feeding is not contacted with chip during cleaning.Conveyer belt movement makes liquid feeding be uniformly distributed chip list during liquid feeding
Face.Aspirating needle then controls lifting by cleaning nozzle lifting motor 32, and when imbibition drops to the cleaning waste liquid suction port 62 of chip edge
Imbibition, waste liquid suction port when cleaning waste liquid suction port 62 is as cleaning, bottom are less than chip surface, and it is useless that aspirating needle drops to cleaning
62 imbibition of liquid suction port.It is exactly one side liquid feeding, while aspirating needle drops to the process of imbibition in recess hole to clean chip processes.
According to above-mentioned principle, the present invention can also carry out the above embodiment appropriate change and modification.Therefore, this hair
It is bright to be not limited to specific embodiment disclosed and described above, some modifications and changes of the present invention should also be as to fall into this
In the scope of the claims of invention.
In practice, conveyer belt 24 uses belt, and the rotation of conveyer belt 24 is controlled using motor.Band driving wheel on motor, wheel
Above cover belt.Sample needle is provided with above 21 right end of feeding-passage, this sample needle is after chip is put on machine, for the first time
Liquid is added in use.I.e. liquid feeding pin is suspended from above chip, adds in liquid.During sample-adding, chip passes through 21 belt of feeding-passage
Movement is to left movement, and by adjusting sample-adding and chip movement speed, chip just moves a chip position at the end of sample-adding.Core
Piece first since 21 right end of feeding-passage, after reaching 21 left side of feeding-passage, is sent to 22 left side of flushing channel, clearly by manipulator
The cleaning head 3 for washing 22 left side of channel starts to clean.Then chip is transmitted to 22 right side of flushing channel, is sent to and blows further through manipulator
23 right side of wind channel, the cleaning head 3 on 23 right side of air blasting passage are cleaned again.3 structure of two of which cleaning head is similar.Due to adopting
With streamlined operation mode, technological requirement has two step cleaning processes, i.e. chip is primary by the cleaning head 3 in 22 left side of flushing channel
After cleaning, wait a few minutes, second of the cleaning positions to be walked automatically to the cleaning head 3 on the right side of air blasting passage 23 carry out second it is clear
It washes.Although cleaning machinery process is the same twice, both sides cleaning solution and differ used by cleaning.After having cleaned, chip
The left side of air blasting passage 23 is sent to, first 4 is dried up by drying.So far chip is just automatically performed entire cleaning process.
Claims (8)
1. a kind of cleaning structure of hybridization instrument, including horizontal-extending Transfer pipe (2) on pedestal (1) and pedestal (1), feature
It is：The Transfer pipe (2) includes feeding-passage (21), flushing channel (22), air blasting passage (23) successively from front to back,
Cleaning head (3), the air blasting passage are respectively arranged on the right side of the left side of the flushing channel (22) and air blasting passage (23)
(23) blowing head (4) is provided on the left of.
2. a kind of cleaning structure of hybridization instrument according to claim 1, it is characterised in that：Before the Transfer pipe (2)
Both sides are respectively arranged with conveyer belt (24) afterwards, and support salient point (25) is distributed on the conveyer belt (24).
3. a kind of cleaning structure of hybridization instrument according to claim 1, it is characterised in that：The blowing head (4) includes
Compressor (41), air knife lifting motor (42), air knife supporting rack (43) and air knife guide bracket (44), the air knife guide bracket (44)
On be provided with air knife lifting guide rail (45), air knife supporting rack (43) lower end is provided with drying process aspirating needle (46) and air knife.
4. a kind of cleaning structure of hybridization instrument according to claim 1, it is characterised in that：The cleaning head (3) includes
Water pump (31), cleaning nozzle lifting motor (32), cleaning nozzle supporting rack (33) and cleaning nozzle guide bracket (34), the cleaning nozzle guiding
Cleaning nozzle guide rails (35) are provided on frame, cleaning nozzle supporting rack (33) lower end is provided with aspirating needle, liquid feeding pin.
5. a kind of cleaning structure of hybridization instrument according to claim 1 or 2, it is characterised in that：On the Transfer pipe (2)
It is provided with heating plate (5).
6. a kind of cleaning structure of hybridization instrument according to claim 1 or 2, it is characterised in that：On the Transfer pipe (2)
The chip groove (6) for chip placement is provided with, the side edge of the chip groove (6) is provided with imbibition recess (61).
7. a kind of cleaning structure of hybridization instrument according to claim 6, it is characterised in that：The imbibition recess (61) includes
There are the cleaning waste liquid suction port (62) for cleaning head (3) and the drying waste liquid suction port (63) for blowing head (4).
8. a kind of cleaning structure of hybridization instrument according to claim 3, it is characterised in that：Air knife guide bracket (44) packet
Include the air knife translation motor (48) on air knife level frame (47) and air knife level frame (47).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610415365.XA CN105936873B (en) | 2016-06-13 | 2016-06-13 | A kind of cleaning structure of hybridization instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610415365.XA CN105936873B (en) | 2016-06-13 | 2016-06-13 | A kind of cleaning structure of hybridization instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105936873A CN105936873A (en) | 2016-09-14 |
| CN105936873B true CN105936873B (en) | 2018-06-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610415365.XA Active CN105936873B (en) | 2016-06-13 | 2016-06-13 | A kind of cleaning structure of hybridization instrument |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245440B (en) * | 2017-06-27 | 2024-04-23 | 珠海意动智能装备有限公司 | Transfer assembly and chip detection device |
| CN107356772B (en) * | 2017-06-27 | 2024-05-17 | 珠海意动智能装备有限公司 | Cleaning liquid changing mechanism, chip detection device and control method thereof |
| CN108949553B (en) * | 2018-08-23 | 2024-04-30 | 苏州英芮诚生化科技有限公司 | Waste liquid disposal device for nucleic acid extraction instrument |
| CN110244068A (en) * | 2019-05-06 | 2019-09-17 | 珠海意动智能装备有限公司 | Chip processing mechanism, chip-detecting apparatus and its control method |
| CN111849750B (en) * | 2020-06-23 | 2022-10-28 | 福建工程学院 | Automatic detection system of genotyping chip |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101063078A (en) * | 2007-04-20 | 2007-10-31 | 博奥生物有限公司 | Biological chip hybrid cleaning device |
| CN101085974A (en) * | 2007-06-26 | 2007-12-12 | 北京和利时系统工程股份有限公司 | Biochip hybridization procedure optimization control method and apparatus employing the method |
| CN101942385A (en) * | 2009-07-06 | 2011-01-12 | 中山大学达安基因股份有限公司 | Full-automatic hybridization oven and implementation method and application thereof |
| CN102134552A (en) * | 2010-12-31 | 2011-07-27 | 福建泰普生物科学有限公司 | Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same |
| CN205974519U (en) * | 2016-06-13 | 2017-02-22 | 珠海赛乐奇生物技术股份有限公司 | Washing structure of hybridization appearance |
-
2016
- 2016-06-13 CN CN201610415365.XA patent/CN105936873B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101063078A (en) * | 2007-04-20 | 2007-10-31 | 博奥生物有限公司 | Biological chip hybrid cleaning device |
| CN101085974A (en) * | 2007-06-26 | 2007-12-12 | 北京和利时系统工程股份有限公司 | Biochip hybridization procedure optimization control method and apparatus employing the method |
| CN101942385A (en) * | 2009-07-06 | 2011-01-12 | 中山大学达安基因股份有限公司 | Full-automatic hybridization oven and implementation method and application thereof |
| CN102134552A (en) * | 2010-12-31 | 2011-07-27 | 福建泰普生物科学有限公司 | Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same |
| CN205974519U (en) * | 2016-06-13 | 2017-02-22 | 珠海赛乐奇生物技术股份有限公司 | Washing structure of hybridization appearance |
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| CN105936873A (en) | 2016-09-14 |
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