CN112275455A - 一种基于超速离心技术的外泌体颗粒筛选装置 - Google Patents
一种基于超速离心技术的外泌体颗粒筛选装置 Download PDFInfo
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- CN112275455A CN112275455A CN202011081544.7A CN202011081544A CN112275455A CN 112275455 A CN112275455 A CN 112275455A CN 202011081544 A CN202011081544 A CN 202011081544A CN 112275455 A CN112275455 A CN 112275455A
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- fixedly arranged
- test tube
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- liquid pumping
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 16
- 239000002245 particle Substances 0.000 title claims abstract description 14
- 238000012216 screening Methods 0.000 title claims abstract description 13
- 238000005199 ultracentrifugation Methods 0.000 title claims abstract description 13
- 238000005516 engineering process Methods 0.000 title claims abstract description 12
- 238000012360 testing method Methods 0.000 claims abstract description 67
- 239000002244 precipitate Substances 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims description 52
- 238000005086 pumping Methods 0.000 claims description 51
- 238000005406 washing Methods 0.000 claims description 32
- 238000010926 purge Methods 0.000 claims description 16
- 238000004804 winding Methods 0.000 claims description 10
- 230000005540 biological transmission Effects 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 4
- 230000017105 transposition Effects 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims 1
- 238000007873 sieving Methods 0.000 claims 1
- 238000007664 blowing Methods 0.000 abstract description 11
- 238000012546 transfer Methods 0.000 abstract description 3
- 230000010354 integration Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 22
- 239000013049 sediment Substances 0.000 description 18
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 16
- 239000002953 phosphate buffered saline Substances 0.000 description 16
- 238000010009 beating Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/06—Other accessories for centrifuges for cleaning bowls, filters, sieves, inserts, or the like
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/08—Arrangement or disposition of transmission gearing ; Couplings; Brakes
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- Sampling And Sample Adjustment (AREA)
Abstract
Description
Claims (5)
Priority Applications (1)
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CN202011081544.7A CN112275455B (zh) | 2020-10-12 | 2020-10-12 | 一种基于超速离心技术的外泌体颗粒筛选装置 |
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CN202011081544.7A CN112275455B (zh) | 2020-10-12 | 2020-10-12 | 一种基于超速离心技术的外泌体颗粒筛选装置 |
Publications (2)
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CN112275455A true CN112275455A (zh) | 2021-01-29 |
CN112275455B CN112275455B (zh) | 2021-10-01 |
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CN202011081544.7A Expired - Fee Related CN112275455B (zh) | 2020-10-12 | 2020-10-12 | 一种基于超速离心技术的外泌体颗粒筛选装置 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112973975A (zh) * | 2021-02-24 | 2021-06-18 | 安徽中科中佳科学仪器有限公司 | 一种自洁净过滤分离的实验室用高速离心机 |
CN114700186A (zh) * | 2022-03-17 | 2022-07-05 | 中国科学院海洋研究所 | 一种刺参体液样品外泌体分离的方法 |
CN115069427A (zh) * | 2022-05-24 | 2022-09-20 | 徐腾 | 一种检验试管离心分液装置 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5445593A (en) * | 1992-08-14 | 1995-08-29 | Fresenius Ag | Method and apparatus for the continuous conditioning of a cell suspension |
CN106124282A (zh) * | 2016-07-26 | 2016-11-16 | 广州海力特生物科技有限公司 | 一种叠层离心过滤分离提取外泌体的方法 |
CN108240929A (zh) * | 2017-12-07 | 2018-07-03 | 张荷友 | 一种用于果蔬食品安全检测的样品处理装置 |
CN207958391U (zh) * | 2017-12-21 | 2018-10-12 | 首都医科大学附属北京儿童医院 | 一种外泌体提取装置 |
CN211302910U (zh) * | 2019-12-04 | 2020-08-21 | 天津金域医学检验实验室有限公司 | 一种实验室用电动吹打混匀装置 |
CN211620485U (zh) * | 2019-11-18 | 2020-10-02 | 浙江卫未生物医药科技有限公司 | 一种外泌体提取分离专用超滤、超离一体装置 |
-
2020
- 2020-10-12 CN CN202011081544.7A patent/CN112275455B/zh not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5445593A (en) * | 1992-08-14 | 1995-08-29 | Fresenius Ag | Method and apparatus for the continuous conditioning of a cell suspension |
CN106124282A (zh) * | 2016-07-26 | 2016-11-16 | 广州海力特生物科技有限公司 | 一种叠层离心过滤分离提取外泌体的方法 |
CN108240929A (zh) * | 2017-12-07 | 2018-07-03 | 张荷友 | 一种用于果蔬食品安全检测的样品处理装置 |
CN207958391U (zh) * | 2017-12-21 | 2018-10-12 | 首都医科大学附属北京儿童医院 | 一种外泌体提取装置 |
CN211620485U (zh) * | 2019-11-18 | 2020-10-02 | 浙江卫未生物医药科技有限公司 | 一种外泌体提取分离专用超滤、超离一体装置 |
CN211302910U (zh) * | 2019-12-04 | 2020-08-21 | 天津金域医学检验实验室有限公司 | 一种实验室用电动吹打混匀装置 |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112973975A (zh) * | 2021-02-24 | 2021-06-18 | 安徽中科中佳科学仪器有限公司 | 一种自洁净过滤分离的实验室用高速离心机 |
CN112973975B (zh) * | 2021-02-24 | 2022-08-02 | 安徽中科中佳科学仪器有限公司 | 一种自洁净过滤分离的实验室用高速离心机 |
CN114700186A (zh) * | 2022-03-17 | 2022-07-05 | 中国科学院海洋研究所 | 一种刺参体液样品外泌体分离的方法 |
CN114700186B (zh) * | 2022-03-17 | 2023-04-18 | 中国科学院海洋研究所 | 一种刺参体液样品外泌体分离的方法 |
CN115069427A (zh) * | 2022-05-24 | 2022-09-20 | 徐腾 | 一种检验试管离心分液装置 |
CN115069427B (zh) * | 2022-05-24 | 2024-05-24 | 金昌中圣基新材料有限责任公司 | 一种检验试管离心分液装置 |
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CN112275455B (zh) | 2021-10-01 |
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CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Fu Yunfeng Inventor after: Cao Yanming Inventor after: Tang Youqun Inventor after: Lu Xiaoyan Inventor before: Lu Xiaoyan |
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TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20210908 Address after: 410000 room 1004, building 1, No. 28, Hualu Road, Yuelu District, Changsha City, Hunan Province Applicant after: Tang Youqun Address before: 510000 a910, No.3, Juquan Road, Huangpu District, Guangzhou City, Guangdong Province Applicant before: Guangzhou isoda Biomedical Technology Co.,Ltd. |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20211001 |