CN112274453A

CN112274453A - Skin-care matrix with whitening, anti-allergy and anti-aging functions and preparation method and application thereof

Info

Publication number: CN112274453A
Application number: CN202011231650.9A
Authority: CN
Inventors: 裴运林; 聂艳峰; 郭朝万; 胡露; 蒲艳; 王娟
Original assignee: Guangdong Marubi Biological Technology Co Ltd
Current assignee: Guangdong Marubi Biological Technology Co Ltd
Priority date: 2020-11-06
Filing date: 2020-11-06
Publication date: 2021-01-29
Anticipated expiration: 2040-11-06
Also published as: CN112274453B

Abstract

The invention relates to a skin-care matrix with whitening, anti-allergy and anti-aging functions, a preparation method and application thereof, wherein the skin-care matrix comprises the following components: geranium sunflower oil, chamomile oil, vanillyl butyl ether, oat protein hydrolysate, oat beta-glucan, hydrogenated polyisobutene, sodium hyaluronate and water. The skin care matrix related by the invention is scientifically matched by selecting seven specific components of pelargonium roseum oil, chrysanthemum morifolium oil, vanillyl butyl ether, oat protein hydrolysate, oat beta-glucan, hydrogenated polyisobutene and sodium hyaluronate, and has good synergistic effect on whitening, anti-allergy and anti-aging effects.

Description

Skin-care matrix with whitening, anti-allergy and anti-aging functions and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, relates to a skin-care matrix and a preparation method and application thereof, and particularly relates to a skin-care matrix with whitening, anti-allergy and anti-aging functions and a preparation method and application thereof.

Background

Skin aging is a vital stage accompanying human life activities and is influenced by various aspects such as genetic factors, environmental factors, complex morphological structures of the whole body, and the like. In recent years, with the rise of dietary culture and the change of living habits of people, a series of problems of exogenous accelerated aging, color spots, dark yellow and the like appear on the skins of a plurality of people, and characteristics of wrinkles, skin laxity and the like begin to be generated.

With the development of the science and technology of cosmetics, various skin care products have different quality, and a series of cosmetics with the functions of resisting aging, preserving moisture, whitening, resisting inflammation, repairing and the like are also endless. However, although some cosmetics have efficacy due to the addition of more active ingredients, many people are still sensitive to allergic phenomena such as erythema and swelling pain, and the safety is questioned. In addition, although the components such as vitamin C, hormone, polypeptide and the like which play an anti-aging effect in cosmetics at present can show obvious effects in a short time, most of the compounds have the defects of easy accumulation, difficult decomposition, low safety, poor stability and the like, and cause toxicity to human bodies.

On the other hand, because of the self-barrier function of human skin, general active ingredients are difficult to really enter the deep layer of the skin to play a role, and only can play a role in simply preserving moisture. In order to enhance the penetration and absorption of nutrient components in the skin care product, the external steam engine can be used for assisting in heating, so that a better beautifying effect is achieved. Products of many beauty salons achieve the effect of promoting absorption by means of the functions of introduction instruments (electrical conduction, ultrasonic introduction, radio frequency introduction, microcrystal introduction, micro-needle introduction and the like), but the long-term disordered use of the beauty instruments can cause certain damage to skin, and the use method is not simple and convenient, takes time, and has very limited effect of promoting absorption.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a skin-care matrix and a preparation method and application thereof, and particularly provides a skin-care matrix with whitening, anti-allergy and anti-aging functions and a preparation method and application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a skin care base with whitening, anti-allergy, anti-aging properties, the skin care base comprising: geranium sunflower oil, chamomile oil, vanillyl butyl ether, oat protein hydrolysate, oat beta-glucan, hydrogenated polyisobutene, sodium hyaluronate and water.

The skin care matrix related by the invention is scientifically matched by selecting seven specific components of pelargonium roseum oil, chrysanthemum morifolium oil, vanillyl butyl ether, oat protein hydrolysate, oat beta-glucan, hydrogenated polyisobutene and sodium hyaluronate, and has good synergistic effect on whitening, anti-allergy and anti-aging effects. The oat protein hydrolysate and the oat beta-glucan exert whitening, anti-allergy and anti-aging effects of the oat protein hydrolysate and the oat beta-glucan, and the addition of the pelargonium roseum oil and the florists chrysanthemum oil further promotes the effects of the oat protein hydrolysate and the oat beta-glucan, so that the oat protein hydrolysate and the oat beta-glucan have obvious synergistic effects on whitening, anti-allergy and anti-aging; the vanillyl butyl ether is used as a heating agent, and is matched with geranium and geranium oil and chamomile oil which have the heating effect and the allergy relieving effect, so that the irritation of the vanillyl butyl ether can be relieved, the skin care matrix is milder, functional components in the matrix are promoted to be absorbed by the skin more easily by adding the heating agent, and the whitening, anti-allergy and anti-aging effects are more remarkable; hydrogenated polyisobutene and sodium hyaluronate exert skin softening and moisturizing effects.

Preferably, the skin care base comprises the following components in parts by weight: 10-25 parts of pelargonium graveolens sunflower oil, 5-15 parts of chamomile oil, 5-15 parts of vanillyl butyl ether, 30-45 parts of oat protein hydrolysate, 20-30 parts of oat beta-glucan, 1-5 parts of hydrogenated polyisobutene, 1-5 parts of sodium hyaluronate and 30-50 parts of water.

The seven components of the pelargonium roseum sunflower oil, the chamomile oil, the vanillyl butyl ether, the oat protein hydrolysate, the oat beta-glucan, the hydrogenated polyisobutene and the sodium hyaluronate in the skin care matrix are scientifically matched according to the weight parts, so that the whitening, anti-allergy and anti-aging effects are more remarkable.

The weight parts of the pelargonium roseum sunflower oil can be 10 parts, 12 parts, 14 parts, 15 parts, 18 parts, 20 parts, 22 parts or 25 parts and the like.

The weight parts of the chrysanthemum morifolium oil can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 12 parts or 15 parts and the like.

The weight portion of the vanillyl butyl ether can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 12 parts or 15 parts and the like.

The oat protein hydrolysate can be 30 parts, 32 parts, 35 parts, 38 parts, 40 parts, 42 parts or 45 parts by weight.

The oat beta-glucan can be 20 parts, 22 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts or 30 parts by weight.

The hydrogenated polyisobutene can be present in 1, 2, 3, 4 or 5 parts by weight.

The sodium hyaluronate can be 1 part, 2 parts, 3 parts, 4 parts or 5 parts by weight.

The water may be present in an amount of 30, 32, 35, 38, 40, 42, 43, 45, 46, 48, or 50 parts by weight.

Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.

In the invention, the oat protein hydrolysate is a product obtained by sequentially carrying out enzymolysis on oat powder serving as a raw material by adopting alpha-amylase and compound protease; the compound protease consists of trypsin, papain and carboxypeptidase A.

The oat protein hydrolysate creatively adopts a mode of sequentially carrying out enzymolysis on alpha-amylase and compound protease, so that the obtained product is added into the skin-care matrix to play more excellent whitening, anti-allergy and anti-aging effects. The specific enzymolysis mode is as follows:

preferably, the oat protein hydrolysate is prepared by the following method:

(S1) mixing oat flour with water, adding alpha-amylase for enzymolysis, adjusting the pH value after the enzymolysis is finished, standing, and centrifuging to obtain a precipitate;

(S2) mixing the precipitate obtained in the step (S1) with water, adding compound protease for enzymolysis to obtain an enzymolysis liquid;

(S3) inactivating enzyme of the enzymolysis liquid obtained in the step (S2), concentrating and drying to obtain the oat protein hydrolysate.

Preferably, the amount of water used in step (S1) is 5-10 times, for example, 5 times, 6 times, 7 times, 8 times, 9 times or 10 times of the oat flour, and other specific values within the above numerical range can be selected, and are not described in detail herein.

Preferably, the amount of the alpha-amylase used in the step (S1) is 0.05-0.5% of the mass of the oat flour aqueous solution, such as 0.05%, 0.08%, 0.1%, 0.2%, 0.3%, 0.4%, or 0.5%, and other specific values within the above range can be selected, and are not repeated herein.

Preferably, in step (S1), the temperature at which the α -amylase performs enzymatic hydrolysis is 55 to 70 ℃, for example, 55 ℃, 60 ℃, 65 ℃ or 70 ℃, the time is 1 to 3 hours, for example, 1 hour, 1.5 hours, 2 hours, 2.5 hours or 3 hours, and the pH is 8 to 11, for example, 8, 9, 10 or 11, and other specific values within the above range may be selected, which is not repeated herein.

Preferably, in step (S1), after the enzymatic hydrolysis is completed, the pH is adjusted to 2-5, for example, pH 2, pH 3, pH 4, or pH 5, and other specific values within the above range may be selected, which is not described herein again.

Preferably, the centrifugation speed in the step (S1) is 3000-.

Preferably, the amount of water used in step (S2) is 1-5 times, for example, 1 time, 2 times, 3 times, 4 times, 4.5 times or 5 times of the precipitation mass, and other specific values within the above numerical range can be selected, and are not described in detail herein.

Preferably, the amount of the compound protease used in the step (S2) is 0.05-0.15% of the mass of the aqueous precipitation solution, such as 0.05%, 0.06%, 0.08%, 0.10%, 0.12%, 0.13%, 0.14%, or 0.15%, and other specific values within the above-mentioned value range can be selected, and are not repeated herein.

Preferably, in step (S2), the temperature at which the compound protease performs the enzymatic hydrolysis is 40-60 ℃, for example, 40 ℃, 45 ℃, 50 ℃, 55 ℃ or 60 ℃, the time is 5-10h, for example, 5h, 6h, 7h, 8h, 9h, 10h, etc., and the pH is 5-8, for example, pH 5, pH 6, pH 7, pH 8, etc., and other specific values within the above range may be selected, which is not repeated herein.

Preferably, the ratio of the trypsin, the papain and the carboxypeptidase A in the composite protease in the step (S2) is (2-3):1: 1 (1-2), such as 2:1:1, 2:1:2, 3:1:1 or 3:1:2, and other specific values in the above numerical range can be selected, and are not described in detail herein.

Preferably, the concentration in step (S3) is vacuum concentration, and is concentrated to 20-60% of the solid content, for example, 20%, 30%, 40%, 50% or 60%, and other specific values within the above numerical range can be selected, and are not described herein again.

Preferably, the drying in step (S3) is performed by spray drying, the temperature of the inlet is 170-200 ℃, such as 170 ℃, 180 ℃, 190 ℃ or 200 ℃, and the temperature of the outlet is 70-100 ℃, such as 70 ℃, 80 ℃, 90 ℃ or 100 ℃, and other specific values in the above numerical range can be selected, and are not repeated herein.

As a preferred technical scheme of the invention, the oat protein hydrolysate is prepared by the following method:

(S1) mixing oat flour with water which is 5-10 times of the oat flour in mass, adding 0.05-0.5% of alpha-amylase to carry out enzymolysis for 1-3h at 55-70 ℃ and under the condition that the pH value is 8-11, adjusting the pH value to 2-5 after the enzymolysis is finished, standing, and centrifuging at the rotating speed of 3000-5000rpm for 15-30min to obtain a precipitate;

(S2) mixing the precipitate obtained in the step (S1) with water with the mass 1-5 times that of the precipitate, adding 0.05-0.15% of compound protease (the dosage ratio of trypsin, papain and carboxypeptidase A is (2-3):1 (1-2)) and carrying out enzymolysis for 5-10h at the temperature of 40-60 ℃ and under the pH value of 5-8 to obtain an enzymolysis solution;

(S3) deactivating enzyme of the enzymatic hydrolysate obtained in the step (S2), vacuum concentrating to 50% of solid content, and spray drying (the temperature of a feed inlet is 180 ℃ and the temperature of a discharge outlet is 90 ℃) to obtain the oat protein hydrolysate.

In a second aspect, the present invention provides a method for preparing the skin care matrix with whitening, anti-allergy and anti-aging effects, the method comprising the following steps:

(1) mixing hydrogenated polyisobutene, sodium hyaluronate and water, and stirring to obtain a solution A;

(2) mixing the pelargonium graveolens sunflower oil, the chamomile oil and the vanillyl butyl ether with the solution A obtained in the step (1), and stirring to obtain a solution B;

(3) and (3) finally, mixing the oat protein hydrolysate and the oat beta-glucan with the solution B obtained in the step (2), and stirring to obtain the skin-care matrix with whitening, anti-allergy and anti-aging functions.

Preferably, the stirring temperature in step (1) is 40-50 ℃, such as 40 ℃, 42 ℃, 45 ℃, 48 ℃ or 50 ℃, etc., the time is 30-60min, such as 30min, 40min, 50min, 60min, etc., the stirring speed is 1000-2000rpm, such as 1000rpm, 1200rpm, 1500rpm, 1800rpm or 2000rpm, etc., and other specific values in the above numerical range can be selected, which is not described in detail herein.

Preferably, the stirring temperature in step (2) is 40-50 ℃, for example, 40 ℃, 42 ℃, 45 ℃, 48 ℃ or 50 ℃, etc., the time is 10-20min, for example, 10min, 12min, 15min, 18min or 20min, etc., the stirring speed is 1000-3000rpm, for example, 1000rpm, 1500rpm, 2000rpm, 2500rpm or 3000rpm, etc., and other specific points in the above numerical range can be selected, which is not described in detail herein.

Preferably, the stirring temperature in step (3) is 15-25 ℃, for example, 15 ℃, 18 ℃, 20 ℃, 23 ℃ or 25 ℃, etc., the time is 15-25min, for example, 15min, 18min, 20min, 23min or 25min, etc., the stirring speed is 500-1000rpm, for example, 500rpm, 600rpm, 700rpm, 800rpm, 900rpm, 1000rpm, etc., and other specific points in the above numerical range can be selected, which is not described in detail herein.

As a preferred technical scheme of the invention, the preparation method of the skin-care matrix with whitening, anti-allergy and anti-aging functions comprises the following steps:

(1) mixing hydrogenated polyisobutene, sodium hyaluronate and water, and stirring at the speed of 1000-2000rpm at 40-50 ℃ for 30-60min to obtain a solution A;

(2) mixing Pelargonium graveolens sunflower oil, Chrysanthemum morifolium oil and vanillyl butyl ether with the solution A obtained in the step (1), and stirring at the temperature of 40-50 ℃ and the speed of 1000-3000rpm for 10-20min to obtain a solution B;

(3) and (3) finally, mixing the oat protein hydrolysate and the oat beta-glucan with the solution B obtained in the step (2), and stirring at the speed of 500-1000rpm at 15-25 ℃ for 15-25min to obtain the skin-care matrix with whitening, anti-allergy and anti-aging effects.

In a third aspect, the invention provides an application of the skin care matrix with whitening, anti-allergy and anti-aging functions in preparation of cosmetics.

The cosmetic is, for example, a lotion containing 10-20 wt% of the skin care base, wherein the lotion consists of the following components in percentage by weight:

the component A comprises: 5.0-10.0% of glycerin, 5.0-8.0% of biological propylene glycol and 9410.05-0.15% of carbomer;

and B component: 0.1-0.2% of methylparaben, 0.2-0.5% of panthenol and 60.88-79.56% of deionized water;

and C, component C: 0.01-0.03% of essence, 0.05-0.15% of PEG-40 hydrogenated castor oil and 10-20% of skin care matrix;

and (D) component: 0.03-0.09% of triethanolamine.

The cosmetic is, for example, a skin cream containing 10-20 wt% of the skin care matrix, and the skin cream comprises the following components in percentage by weight:

the component A comprises: 1.0-2.5% of lecithin, 2-5% of jojoba oil, 1-3% of wheat germ oil and 0.5-1.5% of octadecanol;

and B component: 0.5-1.0% of acrylic polymer, 0.1-0.2% of methyl paraben, 60.88-79.56% of deionized water and 5-10% of butanediol;

and C, component C: 0.3-0.5% of phenoxyethanol and 1-2% of aloe extract;

and (D) component: the skin care matrix is 10-20%.

Compared with the prior art, the invention has the following beneficial effects:

Detailed Description

To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

The following examples or comparative examples relate to Pelargonium graveolens oil obtained from Cyclobalanopsis aromatica (EINECS: 290-140-0, CAS: 8000-46-2), and Chrysanthemum morifolium oil obtained from Ishiki Biotech, Inc. of Shanghai (CAS: 68916-68-7).

Preparation example 1

The preparation example prepares an oat protein hydrolysate by the following method:

(S1) mixing oat flour with deionized water 8 times the weight of the oat flour, adding 0.1% of alpha-amylase to perform enzymolysis for 2 hours at 60 ℃ and pH 9, adjusting the pH value to 4 after the enzymolysis is finished, standing, and centrifuging at 3000rpm for 20min to obtain precipitate;

(S2) mixing the precipitate obtained in the step (S1) with water with the mass 5 times that of the precipitate, adding 0.1% of compound protease (the dosage ratio of trypsin, papain to carboxypeptidase A is 3:1:2) to carry out enzymolysis for 8 hours at 50 ℃ and the pH value of 6 to obtain an enzymolysis liquid;

Preparation example 2

(S1) mixing oat flour with deionized water 10 times the weight of the oat flour, adding 0.5% of alpha-amylase to perform enzymolysis for 3 hours at 70 ℃ and at a pH value of 10, adjusting the pH value to 5 after the enzymolysis is finished, standing, and centrifuging at 3000rpm for 30min to obtain a precipitate;

(S2) mixing the precipitate obtained in the step (S1) with water with the mass being 3 times that of the precipitate, adding 0.15% of compound protease (the dosage ratio of the trypsin to the papain to the carboxypeptidase A is 2:1:1) to carry out enzymolysis for 10 hours at the temperature of 60 ℃ and the pH value of 7 to obtain an enzymolysis liquid;

Preparation example 3

(S1) mixing oat flour with deionized water 5 times the weight of the oat flour, adding 0.1% of alpha-amylase to perform enzymolysis for 2 hours at 55 ℃ and pH 11, adjusting the pH value to 3 after the enzymolysis is finished, standing, and centrifuging at 4000rpm for 20min to obtain a precipitate;

(S2) mixing the precipitate obtained in the step (S1) with water with the mass 2 times that of the precipitate, adding 0.05% of compound protease (the dosage ratio of trypsin, papain to carboxypeptidase A is 2:1:2) to carry out enzymolysis for 10 hours at 50 ℃ and under the pH value of 7 to obtain an enzymolysis liquid;

Preparation example 4

the difference from preparation example 1 is that only trypsin, a single protease, is used, and the amount of trypsin is consistent with the total amount of composite protease in preparation example 1, and other conditions are unchanged.

Preparation example 5

the difference from the preparation example 1 is that only the single protease papain is used, the dosage is consistent with the total amount of the compound protease in the preparation example 1, and other conditions are kept unchanged.

Preparation example 6

the difference from preparation example 1 is that only the single protease carboxypeptidase A is used, and the amount of the carboxypeptidase A is consistent with the total amount of the compound protease in preparation example 1, and other conditions are unchanged.

Preparation example 7

the difference from the preparation example 1 is only that the dosage ratio of the trypsin, the papain and the carboxypeptidase A is 1:1:3, the dosage is consistent with the total amount of the compound protease in the preparation example 1, and other conditions are kept unchanged.

Preparation example 8

the difference from the preparation example 1 is only that the dosage ratio of trypsin, papain and carboxypeptidase A is 4:1:1, the dosage is consistent with the total amount of the compound protease in the preparation example 1, and other conditions are kept unchanged.

Example 1

The embodiment provides a skin-care matrix with whitening, anti-allergy and anti-aging effects, which comprises 15 parts of pelargonium graveolens sunflower oil, 10 parts of chamomile oil, 10 parts of vanillyl butyl ether, 35 parts of oat protein hydrolysate prepared in preparation example 1, 25 parts of oat beta-glucan, 3 parts of hydrogenated polyisobutene, 4 parts of sodium hyaluronate and 45 parts of water.

The preparation method comprises the following steps:

(1) mixing hydrogenated polyisobutene, sodium hyaluronate and water, and stirring at the speed of 1500rpm at 45 ℃ for 50min to obtain a solution A;

(2) mixing the pelargonium roseum sunflower oil, the chamomile oil and the vanillyl butyl ether with the solution A obtained in the step (1), and stirring at the speed of 2000rpm at 45 ℃ for 15min to obtain a solution B;

(3) and (3) finally, mixing the oat protein hydrolysate and the oat beta-glucan with the solution B obtained in the step (2), and stirring at the speed of 800rpm for 20min at the temperature of 20 ℃ to obtain the skin-care matrix with whitening, anti-allergy and anti-aging effects.

Example 2

The embodiment provides a skin-care matrix with whitening, anti-allergy and anti-aging effects, which comprises 12 parts of pelargonium graveolens sunflower oil, 15 parts of chamomile oil, 5 parts of vanillyl butyl ether, 45 parts of oat protein hydrolysate prepared in preparation example 2, 20 parts of oat beta-glucan, 5 parts of hydrogenated polyisobutene, 2 parts of sodium hyaluronate and 50 parts of water.

The preparation method comprises the following steps:

(1) mixing hydrogenated polyisobutene, sodium hyaluronate and water, and stirring at the speed of 2000rpm at 40 ℃ for 50min to obtain a solution A;

(2) mixing Pelargonium graveolens sunflower oil, Chrysanthemum morifolium oil and vanillyl butyl ether with the solution A obtained in the step (1), and stirring at 40 ℃ and 3000rpm for 20min to obtain a solution B;

(3) and (3) finally, mixing the oat protein hydrolysate and the oat beta-glucan with the solution B obtained in the step (2), and stirring at the speed of 1000rpm at 15 ℃ for 25min to obtain the skin-care matrix with whitening, anti-allergy and anti-aging effects.

Example 3

The embodiment provides a skin-care matrix with whitening, anti-allergy and anti-aging effects, which comprises 25 parts of pelargonium graveolens sunflower oil, 10 parts of chamomile oil, 5 parts of vanillyl butyl ether, 30 parts of oat protein hydrolysate prepared in preparation example 3, 30 parts of oat beta-glucan, 2 parts of hydrogenated polyisobutene, 5 parts of sodium hyaluronate and 35 parts of water.

The preparation method comprises the following steps:

(1) mixing hydrogenated polyisobutene, sodium hyaluronate and water, and stirring at the speed of 1000rpm at 50 ℃ for 30min to obtain a solution A;

(2) mixing Pelargonium graveolens sunflower oil, Chrysanthemum morifolium oil and vanillyl butyl ether with the solution A obtained in the step (1), and stirring at the speed of 1000rpm at 50 ℃ for 10min to obtain a solution B;

(3) and (3) finally, mixing the oat protein hydrolysate and the oat beta-glucan with the solution B obtained in the step (2), and stirring at the speed of 500rpm at 25 ℃ for 15min to obtain the skin-care matrix with whitening, anti-allergy and anti-aging effects.

Examples 4 to 8

This example provides five skin care bases with whitening, anti-allergy and anti-aging effects, which are different from example 1 only in that oat protein hydrolysate prepared in preparation example 1 is replaced with oat protein hydrolysate prepared in preparation examples 4-8, other conditions are kept unchanged, and the preparation method is the same as example 1.

Comparative example 1

The comparative example provides a skin-care matrix with whitening, anti-allergy and anti-aging effects, which is different from the skin-care matrix in example 1 only in that the matrix does not contain geranium oil, 17.5 parts of chamomile oil and 17.5 parts of vanillyl butyl ether, the other conditions are kept unchanged, and the preparation method is also the same as that in example 1.

Comparative example 2

The comparative example provides a skin-care matrix with whitening, anti-allergy and anti-aging effects, which is different from the skin-care matrix in example 1 only in that the matrix does not contain chamomile oil, 21 parts of geranium oil and 14 parts of vanillyl butyl ether, the other conditions are kept unchanged, and the preparation method is also the same as that in example 1.

Comparative example 3

The comparative example provides a skin-care matrix with whitening, anti-allergy and anti-aging effects, which is different from the skin-care matrix in example 1 only in that the matrix does not contain vanillyl butyl ether, 21 parts of geranium and 14 parts of chamomile oil and the preparation method is the same as that in example 1, and other conditions are kept unchanged.

Application example 1

The application example provides a toning lotion which comprises the following components in percentage by weight:

the component A comprises: 7.5% of glycerin, 6.5% of biological propylene glycol and 9410.1% of carbomer;

and B component: 0.15 percent of methyl paraben, 0.35 percent of panthenol and 70.22 percent of deionized water;

and C, component C: 0.02% of essence, 0.1% of PEG-40 hydrogenated castor oil and 15% of the skin care substrate prepared in example 1;

and (D) component: 0.06 percent of triethanolamine.

The preparation method comprises the following steps:

(1) putting the component A into a container, uniformly dispersing by using a mobile stirrer at the rotating speed of 2500rpm, and stirring for 10min to obtain a component A mixture;

(2) adding deionized water into a water aqua pot, heating to 70 ℃, starting stirring at 1000rpm, adding the mixture of the component A and the rest of the component B, continuously heating to 85 ℃, and keeping the temperature for 30 min;

(3) reducing the stirring speed to 500rpm, starting cooling water to reduce the temperature to 40 ℃, adding the component C (the essence and the PEG-40 hydrogenated castor oil are uniformly mixed in advance) into the water aqua pan, and stirring for 10 min;

(4) and then adding the component D, stirring for 5min, and discharging to obtain the toning lotion.

Application examples 2 to 8

The present application example provides seven kinds of lotions whose components are different from those of application example 1 only in that the skin care base prepared in example 1 was replaced with the skin care bases prepared in examples 2 to 8, and the other conditions were kept unchanged, and the preparation method thereof was referred to application example 1.

Comparative application examples 1 to 3

This comparative application example provides three lotions, the components of which differ from those of application example 1 only in that the skin care base prepared in example 1 was replaced with the skin care bases prepared in comparative examples 1 to 3, and the other conditions were maintained unchanged, and the preparation methods thereof were compared with application example 1.

Evaluation test:

(1) antioxidant efficacy research-DPPH free radical scavenging experiment

47.7g of DPPH reagent is accurately weighed, the volume is determined to be 100mL of brown volumetric flask, 5mL of the brown volumetric flask is determined to be 50mL of brown volumetric flask with absolute ethyl alcohol, and DPPH test solution (47.7mg/L) is obtained. 2mL of the DPPH test solution was removed, and 0.1mL of the product obtained in examples 1 to 8 and comparative examples 1 to 3 was added as shown in the following table, and an appropriate amount of anhydrous ethanol was added to equalize the total mass. Standing in dark for 30min after mixing, measuring absorbance at 517nm with anhydrous ethanol as blank control, and calculating DPPH clearance rate of each extractive solution according to the following formula with vitamin C as control:

DPPH clearance (%) [1- (As-Ar)/a0] × 100%

In the formula: as is the absorbance of the reaction system of the extract and DPPH; ar-absorbance of extract-absolute ethanol; a0-absorbance of blank control absolute ethanol.

The DPPH free radical clearance rate reflects the antioxidant capacity to a certain extent, the higher the DPPH free radical clearance rate is, the better the antioxidant capacity is, and the better the anti-aging effect is, so that the anti-aging effect can be judged through the research on the DPPH free radical clearance capacity of the composition. Vitamin C is a high-efficiency antioxidant accepted by the cosmetic industry, and the antioxidant capacity of the experiment is examined by taking the vitamin C as a control. The test results are shown in table 1:

TABLE 1

As can be seen from Table 1, the skin care substrate of the present invention has a good effect of removing DPPH, and the removal rate is 82.96% or more. Wherein, the clearance rate of example 1 is higher than that of examples 4-8, indicating that the preparation conditions of oat protein hydrolysate in the skin care base significantly affect the efficacy of the final product; the clearance rate of the skin care matrix in the embodiment 1 is higher than that of the skin care matrix in the comparative examples 1-3, so that the synergistic effect of the geranium oil, the white-flowered chrysanthemum oil and the vanillyl butyl ether in the skin care matrix is shown, and the rationality of the compatibility of the components is verified.

(2) Anti-allergic efficacy-Hyaluronidase in vitro inhibition test

Hyaluronic acid is a component that limits the diffusion of water and other extracellular substances in the tissue matrix, and after being hydrolyzed by hyaluronidase, cells become non-viscous, causing degranulation of cells and the exudation of new synthesized mediators, exerting biological effects, resulting in the occurrence of immediate type hypersensitivity. Hyaluronidase is a participant in allergic reactions, has strong allergic-related effects, and can relieve allergic phenomena and improve skin by inhibiting the activity of hyaluronidase.

The anti-allergic effects in vitro of examples 1 to 8 and comparative examples 1 to 3 were examined by the hyaluronidase inhibition method. Taking 0.1mL of CaCl₂Reacting the solution (0.25mmol/L) and 0.5mL of hyaluronidase solution (100U/mL) in water bath at 37 ℃ for 20 min; adding 0.5mL of the extracting solution, and keeping the temperature for 20 min; then adding 0.5mL of sodium hyaluronate solution (0.5g/L), reacting for 30min, taking out and cooling for 5 min; adding 0.1mL of sodium hydroxide solution (0.4mol/L) and 0.5mL of acetylacetone solution (3.5mL of acetylacetone dissolved in 50mL of 1.0mol/L sodium carbonate solution), boiling in water bath for 15min, and immediately transferring to ice water bath for 5 min; 1mL of an Ellisib reagent (0.8g of p-dimethylaminobenzaldehyde dissolved in 15mL of concentrated hydrochloric acid and 15mL of absolute ethanol) was added dropwise, and diluted with 3mL of absolute ethanol, left at 25 ℃ for 20min for color development, and the absorbance was measured at 540nm with dipotassium glycyrrhizinate as a control. The hyaluronidase inhibition rate was calculated according to the following formula:

hyaluronidase inhibition { [ (A-B) - (C-D) ]/(A-B) } × 100%

In the formula: a-absorbance of control solution (the sample solution was replaced with acetic acid buffer solution); b-the absorbance of the control blank solution (the sample solution and the enzyme solution are replaced by acetic acid buffer solution); c-absorbance of the sample solution; d-absorbance of sample blank solution (acetic acid buffer solution was used instead of enzyme solution).

The inhibition rate of each group of samples on hyaluronidase was examined by using 5% dipotassium glycyrrhizinate as a positive control, and the results are shown in table 2:

TABLE 2

As is clear from table 2, the skin care base according to the present invention has a good inhibitory effect on hyaluronidase, and the inhibitory rate is 81.5% or more. Wherein the inhibition rate of example 1 is higher than that of examples 4-8, indicating that the preparation conditions of oat protein hydrolysate in the skin care base significantly affect the efficacy of the final product; the inhibition rate of the example 1 is higher than that of the comparative examples 1-3, which shows that the synergistic effect of the geranium oil, the chamomile oil and the vanillyl butyl ether in the skin care matrix is verified, and the rationality of the compatibility of the components is verified.

(3) Skin whitening efficacy-tyrosinase in vitro inhibition test

The following solutions of 5mM L-tyrosine (L-tyrosine can be dissolved with a small amount of hydrochloric acid and then fixed to volume with PBS buffer solution) and tyrosinase (diluted to 100U/mL with deionized water) were prepared. 0.4mL of sample, 1mL of 0.05mol/L phosphate buffer (pH 6.8), 0.8mL of 0.5mmol/L tyrosine solution, and finally 0.40mL of tyrosinase solution were transferred to a cuvette by a micropipette, and mixed rapidly, and the increase curve of the optical density value at 475nm with time was measured at a constant temperature of 30 ℃ to determine the inhibition ratio from the slope of the curve. Respectively measuring the inhibition rate of the extracts with different concentrations, taking the concentration as an abscissa and the inhibition rate as an ordinate to make a straight line, obtaining a straight line equation, and calculating the concentration of the extract when the inhibition rate is 50 percent, namely the IC 50. The results are shown in Table 3:

TABLE 3

As can be seen from Table 3, the skin care base according to the present invention has a good inhibitory effect on tyrosinase with an IC50 value of 0.842mg/mL or less. Wherein, the IC50 value of example 1 is lower than that of examples 4-8, indicating that the preparation conditions of oat protein hydrolysate in the skin care base significantly affect the efficacy of the final product; the IC50 value of example 1 is lower than that of comparative examples 1-3, which shows the synergistic effect of the geranium oil, the chamomile oil and the vanillyl butyl ether in the skin care matrix, and the rationality of the compatibility of the components is verified.

(4) Haemolysis test of erythrocytes

This item was evaluated for irritation by an erythrocyte hemolysis experiment. The erythrocyte hemolysis test is one of the alternatives of the rabbit eye irritation test (Draize test), and the basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the dissolution amount and denaturation degree of hemoglobin. The RBC test is used internationally for evaluating eye irritation studies on chemicals such as cosmetics and raw materials, and the classification criteria are shown in the following table.

The results of the hemolysis test on erythrocytes for each set of samples are shown in Table 4, where L/D is the ratio of HD50 (the concentration of the sample at which 50% of erythrocytes are hemolyzed) to DI (protein denaturation index).

TABLE 4

As can be seen from Table 4, the skin care matrix of the present invention is mild and non-irritating, and is safe to use.

(5) Human body evaluation test:

160 volunteers aged 20-45, half of all male and female were selected and randomly divided into 8 groups. The left sides of 1-8 groups of volunteers respectively use the lotion prepared by application examples 1-8, the right sides of 1-3 groups of volunteers, 4-6 groups of volunteers and 7-8 groups of volunteers respectively use the lotion prepared by application examples 1-3, the lotion is applied once in the morning and evening every day, 5g of the product is applied to the face each time, the face is massaged for 2 minutes and continuously used for 30 days, the volunteers respectively score the skin whitening ability, the skin elasticity and the allergy relieving effect of the left face and the right face, the maximum score is 5, the minimum score is 1, and the evaluation results are shown in table 5 (average score):

TABLE 5

Sample name	Skin whitening ability	Elasticity of skin	Soothing and sensitizing effect
				Application example 1	4.1	4.4	4.6
Application example 2	4.2	4.1	4.5
				Application example 3	4.5	4.0	4.2
Application example 4	3.8	4.0	4.1
				Application example 5	4.0	3.8	4.2
Application example 6	3.8	3.6	3.9
				Application example 7	4.2	4.2	4.3
Application example 8	4.0	4.1	4.5
				Comparative application example 1	3.3	3.5	3.9
Comparative application example 2	3.7	3.2	3.5
				Comparative application example 3	3.8	3.5	4.0

As can be seen from table 5, the skin care base according to the present invention, when added to a cosmetic product, significantly improved the sensory scores of skin whiteness, elasticity and soothing effect.

The applicant states that the present invention is illustrated by the above examples, but the present invention is not limited to the above examples, i.e. it does not mean that the present invention must be implemented by the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.

It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.

Claims

1. A skin care matrix having whitening, anti-allergy, anti-aging properties, wherein the skin care matrix comprises: geranium sunflower oil, chamomile oil, vanillyl butyl ether, oat protein hydrolysate, oat beta-glucan, hydrogenated polyisobutene, sodium hyaluronate and water.

2. The skin care matrix with whitening, anti-allergy and anti-aging effects as set forth in claim 1, wherein the skin care matrix comprises, in parts by weight: 10-25 parts of pelargonium graveolens sunflower oil, 5-15 parts of chamomile oil, 5-15 parts of vanillyl butyl ether, 30-45 parts of oat protein hydrolysate, 20-30 parts of oat beta-glucan, 1-5 parts of hydrogenated polyisobutene, 1-5 parts of sodium hyaluronate and 30-50 parts of water.

3. The skin care substrate with whitening, anti-allergy and anti-aging effects as claimed in claim 1 or 2, wherein the oat protein hydrolysate is a product obtained by sequentially performing enzymolysis on oat flour as a raw material by using alpha-amylase and compound protease; the compound protease consists of trypsin, papain and carboxypeptidase A.

4. The skin care base with whitening, anti-allergy and anti-aging effects according to claim 3, wherein the oat protein hydrolysate is prepared by the following method:

5. The skin care base with whitening, anti-allergy and anti-aging effects as set forth in claim 4, wherein the amount of water used in the step (S1) is 5-10 times the weight of oat flour;

preferably, the amount of the alpha-amylase used in the step (S1) is 0.05-0.5% of the mass of the oat flour water solution;

preferably, the temperature for enzymolysis by the alpha-amylase in the step (S1) is 55-70 ℃, the time is 1-3h, and the pH value is 8-11;

preferably, in the step (S1), after the completion of the enzymatic hydrolysis, the pH is adjusted to 2 to 5;

preferably, the speed of centrifugation in step (S1) is 3000-5000rpm for 15-30 min.

6. The skin care base with whitening, anti-allergy and anti-aging effects according to claim 4 or 5, wherein the amount of water used in step (S2) is 1-5 times of the mass of the precipitate;

preferably, the amount of the compound protease used in the step (S2) is 0.05-0.15% of the mass of the precipitation aqueous solution;

preferably, the temperature for enzymolysis by the compound protease in the step (S2) is 40-60 ℃, the time is 5-10h, and the pH value is 5-8;

preferably, the dosage ratio of the trypsin, the papain and the carboxypeptidase A in the composite protease in the step (S2) is (2-3):1 (1-2).

7. The skin care base with whitening, anti-allergy and anti-aging effects according to any one of claims 4 to 6, wherein the concentration in the step (S3) is vacuum concentration to 20 to 60% of the solid content;

preferably, the drying in the step (S3) is spray drying, the temperature of the feed inlet is 170-200 ℃, and the temperature of the discharge outlet is 70-100 ℃.

8. The method for preparing the skin care base with whitening, anti-allergy and anti-aging effects according to any one of claims 1 to 7, wherein the method comprises the following steps:

9. The method for preparing the skin care matrix with whitening, anti-allergy and anti-aging effects as claimed in claim 8, wherein the stirring temperature in the step (1) is 40-50 ℃, the time is 30-60min, and the stirring speed is 1000-2000 rpm;

preferably, the stirring temperature in the step (2) is 40-50 ℃, the time is 10-20min, and the stirring speed is 1000-3000 rpm;

preferably, the stirring temperature in the step (3) is 15-25 ℃, the time is 15-25min, and the stirring speed is 500-1000 rpm.

10. Use of the skin care base with whitening, anti-allergy and anti-aging effects according to any one of claims 1 to 7 in the preparation of cosmetics.