CN112273400A - Plant-derived broad-spectrum antibacterial agent and preparation method thereof - Google Patents
Plant-derived broad-spectrum antibacterial agent and preparation method thereof Download PDFInfo
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- CN112273400A CN112273400A CN202011145395.6A CN202011145395A CN112273400A CN 112273400 A CN112273400 A CN 112273400A CN 202011145395 A CN202011145395 A CN 202011145395A CN 112273400 A CN112273400 A CN 112273400A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- Biotechnology (AREA)
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- Medicines Containing Plant Substances (AREA)
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Abstract
The invention discloses a botanical broad-spectrum antibacterial agent and a preparation method thereof, and particularly relates to the field of antibacterial agents, wherein the botanical broad-spectrum antibacterial agent comprises the following components: 8-10 g of gentiana straminea aqueous extract, 8-9 g of chickweed aqueous extract, 3-5 g of stachys japonica aqueous extract, 2-4 g of sanguisorba officinalis aqueous extract, 2-3 g of bodhumnades aqueous extract and 1-2 g of rubus hirsutus aqueous extract. The plant source broad-spectrum antibacterial agent provided by the invention has the advantages of being composed of plant source components, not causing harm to human bodies after long-term use and strong in universality.
Description
Technical Field
The invention relates to the field of antibacterial agents, in particular to a botanical broad-spectrum antibacterial agent and a preparation method thereof.
Background
The antibacterial agent is a medicament for preventing and treating plant diseases caused by various pathogenic microorganisms. An agent having a killing action or growth-inhibiting action on pathogenic microorganisms. Fungicides can be classified according to mode of action, source of raw material and chemical composition. Internationally, they are generally referred to as agents for controlling various types of pathogenic microorganisms. Along with the development of bactericides, virucides, algicides and other subclasses are distinguished.
Most current antimicrobial agents are typically formulated by mixing a variety of functional chemicals. It is sprayed in environment to act on pathogenic microbe slowly and thus has antiseptic effect. However, the conventional antibacterial agents also have the following problems in general use: (1) the long-term inhalation of antibacterial agents formulated with chemical agents by the human body has certain effects on the health of the human body. (2) The traditional antibacterial agent has low universality and low contact killing effect on pathogenic microorganisms.
Disclosure of Invention
The invention provides a plant-derived broad-spectrum antibacterial agent and a preparation method thereof.
In order to achieve the purpose, the invention provides the following technical scheme:
a botanical broad-spectrum antibacterial agent comprises the following components by mass: 8-10 g of gentiana straminea aqueous extract, 8-9 g of chickweed aqueous extract, 3-5 g of stachys japonica aqueous extract, 2-4 g of sanguisorba officinalis aqueous extract, 2-3 g of bodhumnades aqueous extract and 1-2 g of rubus hirsutus aqueous extract.
Preferably, the plant-derived broad-spectrum antibacterial agent further comprises any one of an aqueous extract of raccoon beans, an aqueous extract of jambu, an aqueous extract of tylophora yunnanensis and an aqueous extract of Thladiantha dubia, or a mixture thereof.
Preferably, the plant-derived broad-spectrum antibacterial agent further comprises any one of an aqueous extract of raccoon, an aqueous extract of tylophora yunnanensis and an aqueous extract of Thladiantha dubia Dubiana, or a mixture thereof.
Preferably, the plant-derived broad-spectrum antibacterial agent comprises the following components by mass: 8-10 g of gentiana straminea aqueous extract, 8-9 g of chickweed aqueous extract, 3-5 g of stachys japonica aqueous extract, 2-4 g of garden burnet aqueous extract, 2-3 g of Chinese redpepper aqueous extract, 1-2 g of rubus hirsutus aqueous extract, 1-1.8 g of racoon dog tail bean aqueous extract, 0.5-1 g of tylophora yunnanensis aqueous extract and 0.1-0.5 g of ladyberry dubia aqueous extract.
More preferably, the plant-derived broad-spectrum antibacterial agent comprises the following components by mass: 9g of gentiana straminea aqueous extract, 8.5g of chickweed aqueous extract, 4g of stachys japonica aqueous extract, 3g of garden burnet aqueous extract, 2.5g of Chinese prickly ash aqueous extract, 1.5g of rubus hirsutus aqueous extract, 1.4g of racoon dog tail bean aqueous extract, 0.8g of tylophora yunnanensis aqueous extract and 0.3g of Thladiantha dubia aqueous extract.
The invention also provides a preparation method of the plant-derived broad-spectrum antibacterial agent, which comprises the following steps:
s01, preparing a gentiana straminea aqueous extract:
cleaning and drying the gentiana straminea maxim in preset weight parts, and grinding the gentiana straminea maxim into gentiana straminea maxim powder with the granularity of 100-120 meshes.
Adding purified water into the gentiana straminea maxim powder, carrying out ultrasonic water bath for 60-80 min at 40-60 ℃ under the condition of 300-350W, filtering, taking filtrate, and carrying out rotary steaming till dryness to obtain the gentiana straminea maxim aqueous extract.
S02, preparing the chickweed aqueous extract:
cleaning chickweed, drying, and grinding into chickweed powder with the particle size of 80-100 meshes.
Adding purified water into the chickweed powder, performing ultrasonic water bath for 40-50 min at 50-60 ℃ under 400-500W, filtering, taking filtrate, and performing rotary evaporation to dryness to obtain the chickweed aqueous extract.
S03, preparing a stachys water extract:
cleaning and drying the stachys sieboldii, and grinding the stachys sieboldii into stachys sieboldii powder with the granularity of 60-80 meshes.
Adding purified water into the stachys sieboldii powder, performing ultrasonic water bath for 100-120 min at 80-100 ℃ under the condition of 250-300W, filtering, taking filtrate, and performing rotary evaporation to dryness to obtain the stachys sieboldii aqueous extract.
The preparation method of the big white flower garden burnet aqueous extract, the stone pepper aqueous extract, the rubus hirsutus aqueous extract, the raccoon paguma aqueous extract, the Yunnan tylophora aqueous extract and the penthorum dubia aqueous extract is the same as that of the stachys nigra aqueous extract.
S04, uniformly mixing the prepared components according to a preset proportion to obtain the botanical broad-spectrum antibacterial agent.
Preferably, in step S01, the particle size of the pepper leaf powder is 110 mesh.
Preferably, in step S01, the temperature of the ultrasonic water bath is 50 ℃, the ultrasonic power is 330W, and the time of the ultrasonic water bath is 70 min.
Preferably, in step S02, the chicken intestine chickweed powder has a particle size of 90 mesh, an ultrasonic temperature of 55 deg.C and an ultrasonic power of 450W.
Preferably, in step S03, the stachys powder has a particle size of 70 mesh, an ultrasonic temperature of 90 ℃ and an ultrasonic power of 280W.
The invention has the beneficial effects that:
in the invention, the gentiana straminea aqueous extract can inhibit the growth of pathogenic microorganisms and kill worm eggs in the spraying environment. Meanwhile, the chickweed aqueous extract, the stachys japonica aqueous extract, the garden burnet aqueous extract, the rock pepper aqueous extract and the rubus hirsutus aqueous extract also have broad-spectrum antibacterial effect and can kill various pathogenic microorganisms. Moreover, when people use the plant-derived broad-spectrum antibacterial agent for a long time, the physical and psychological health is not affected, and the plant-derived broad-spectrum antibacterial agent is suitable for long-term use in home environment and office environment.
Detailed Description
Example 1
A botanical broad-spectrum antibacterial agent comprises the following components by mass: 8g of gentiana straminea aqueous extract, 8g of chickweed aqueous extract, 3g of stachys japonica aqueous extract, 2g of garden burnet aqueous extract, 2g of sea pepper aqueous extract, 1g of rubus hirsutus aqueous extract, 1g of paguma larvata aqueous extract, 0.5g of tylophora yunnanensis aqueous extract and 0.1g of ladybell dubia aqueous extract.
The preparation method of the plant-derived broad-spectrum antibacterial agent comprises the following preparation steps:
s01, preparing a gentiana straminea aqueous extract:
cleaning and drying the gentiana straminea maxim in preset weight parts, and grinding the gentiana straminea maxim into gentiana straminea maxim powder with the granularity of 100-120 meshes.
Adding purified water into radix Gentianae Marcrophyllae powder, performing ultrasonic water bath at 50 deg.C and 330W for 70min, filtering, collecting filtrate, and rotary steaming to dry to obtain radix Gentianae Marcrophyllae water extract.
S02, preparing the chickweed aqueous extract:
cleaning chickweed, drying, and grinding into chickweed powder with particle size of 90 mesh.
Adding purified water into chickweed powder, performing ultrasonic water bath at 55 deg.C and 450W for 45min, filtering, collecting filtrate, and rotary steaming to dry to obtain chickweed water extract.
S03, preparing a stachys water extract:
cleaning and drying stachys sieboldii, and grinding to obtain stachys sieboldii powder with the granularity of 70 meshes;
adding purified water into herba Stachydis Japonicae powder, performing ultrasonic water bath at 90 deg.C and 280W for 110min, filtering, collecting filtrate, and rotary steaming to dry to obtain herba Stachydis Japonicae water extract;
the preparation method of the big white flower garden burnet aqueous extract, the stone pepper aqueous extract, the rubus hirsutus aqueous extract, the raccoon paguma aqueous extract, the Yunnan tylophora aqueous extract and the penthorum dubia aqueous extract is the same as that of the stachys nigra aqueous extract;
s04, uniformly mixing the prepared components according to a preset proportion to obtain the botanical broad-spectrum antibacterial agent.
Example 2
A botanical broad-spectrum antibacterial agent comprises the following components by mass: 9g of gentiana straminea aqueous extract, 8.5g of chickweed aqueous extract, 4g of stachys japonica aqueous extract, 3g of garden burnet aqueous extract, 2.5g of Chinese prickly ash aqueous extract, 1.5g of rubus hirsutus aqueous extract, 1.4g of racoon dog tail bean aqueous extract, 0.8g of tylophora yunnanensis aqueous extract and 0.3g of Thladiantha dubia aqueous extract.
The preparation method of the plant-derived broad-spectrum antibacterial agent is the same as that of example 1.
Example 3
A botanical broad-spectrum antibacterial agent comprises the following components by mass: 9.6g of gentiana straminea aqueous extract, 8.7g of chickweed aqueous extract, 4.6g of stachys japonica aqueous extract, 3.6g of sanguisorba grandiflorum aqueous extract, 2.7g of Chinese redpepper aqueous extract, 1.8g of rubus hirsutus aqueous extract, 1.5g of racoon dog tail bean aqueous extract, 0.8g of tylophora yunnanensis aqueous extract and 0.4g of Thladiantha dubia Bunge aqueous extract.
The preparation method of the plant-derived broad-spectrum antibacterial agent is the same as that of example 1.
Example 4
A botanical broad-spectrum antibacterial agent comprises the following components by mass: 10g of gentiana straminea aqueous extract, 9g of chickweed aqueous extract, 5g of stachys japonica aqueous extract, 4g of garden burnet aqueous extract, 3g of sea pepper aqueous extract, 2g of rubus hirsutus aqueous extract, 1.8g of paguma larvata aqueous extract, 1g of tylophora yunnanensis aqueous extract and 0.5g of ladybell dubia aqueous extract.
The preparation method of the plant-derived broad-spectrum antibacterial agent is the same as that of example 1.
Claims (10)
1. A plant-derived broad-spectrum antibacterial agent is characterized by comprising the following components in parts by mass: 8-10 g of gentiana straminea aqueous extract, 8-9 g of chickweed aqueous extract, 3-5 g of stachys japonica aqueous extract, 2-4 g of sanguisorba officinalis aqueous extract, 2-3 g of bodhumnades aqueous extract and 1-2 g of rubus hirsutus aqueous extract.
2. The plant-derived broad-spectrum antibacterial agent of claim 1, further comprising any one of an aqueous extract of raccoon, an aqueous extract of jambu, an aqueous extract of tylophora yunnanensis and an aqueous extract of Thladiantha dubia, or a mixture thereof.
3. The plant-derived broad-spectrum antibacterial agent of claim 2, further comprising any one of an aqueous extract of raccoon, an aqueous extract of tylophora yunnanensis and an aqueous extract of Thladiantha dubia, or a mixture thereof.
4. The plant-derived broad-spectrum antibacterial agent according to any one of claims 1 to 3, which comprises the following components by mass: 8-10 g of gentiana straminea aqueous extract, 8-9 g of chickweed aqueous extract, 3-5 g of stachys japonica aqueous extract, 2-4 g of garden burnet aqueous extract, 2-3 g of Chinese redpepper aqueous extract, 1-2 g of rubus hirsutus aqueous extract, 1-1.8 g of racoon dog tail bean aqueous extract, 0.5-1 g of tylophora yunnanensis aqueous extract and 0.1-0.5 g of ladyberry dubia aqueous extract.
5. The botanical broad-spectrum antibacterial agent according to claim 4, comprising the following components by mass: 9g of gentiana straminea aqueous extract, 8.5g of chickweed aqueous extract, 4g of stachys japonica aqueous extract, 3g of garden burnet aqueous extract, 2.5g of Chinese prickly ash aqueous extract, 1.5g of rubus hirsutus aqueous extract, 1.4g of racoon dog tail bean aqueous extract, 0.8g of tylophora yunnanensis aqueous extract and 0.3g of Thladiantha dubia aqueous extract.
6. A preparation method of a plant-derived broad-spectrum antibacterial agent is characterized by comprising the following steps:
s01, preparing a gentiana straminea aqueous extract:
cleaning and drying the gentiana straminea maxim in preset weight parts, and grinding the gentiana straminea maxim into gentiana straminea maxim powder with the granularity of 100-120 meshes;
adding purified water into the gentiana straminea maxim powder, carrying out ultrasonic water bath for 60-80 min at 40-60 ℃ under the condition of 300-350W, filtering, taking filtrate, and carrying out rotary steaming until the filtrate is dried to obtain the gentiana straminea maxim aqueous extract;
s02, preparing the chickweed aqueous extract:
cleaning chickweed of chicken intestines, drying, and grinding into chickweed powder with the granularity of 80-100 meshes;
adding purified water into the chickweed powder, performing ultrasonic water bath for 40-50 min at 50-60 ℃ under 400-500W, filtering, taking filtrate, and performing rotary evaporation to dryness to obtain the chickweed aqueous extract;
s03, preparing a stachys water extract:
cleaning and drying stachys sieboldii, and grinding to obtain stachys sieboldii powder with the granularity of 60-80 meshes;
adding purified water into the stachys sieboldii powder, performing ultrasonic water bath for 100-120 min at 80-100 ℃ under the condition of 250-300W, filtering, taking filtrate, and performing rotary evaporation to dryness to obtain a stachys sieboldii aqueous extract;
the preparation method of the big white flower garden burnet aqueous extract, the stone pepper aqueous extract, the rubus hirsutus aqueous extract, the raccoon paguma aqueous extract, the Yunnan tylophora aqueous extract and the penthorum dubia aqueous extract is the same as that of the stachys nigra aqueous extract;
s04, uniformly mixing the prepared components according to a preset proportion to obtain the botanical broad-spectrum antibacterial agent.
7. The method for preparing a botanical broad-spectrum antibacterial agent as claimed in claim 6, wherein in step S01, the particle size of the pepper leaf powder is 110 mesh.
8. The method for preparing a botanical broad-spectrum antibacterial agent according to claim 6, wherein in step S01, the temperature of the ultrasonic bath is 50 ℃, the ultrasonic power is 330W, and the time of the ultrasonic bath is 70 min.
9. The method of claim 6, wherein in step S02, the chickweed powder has a particle size of 90 mesh, an ultrasonic temperature of 55 deg.C and an ultrasonic power of 450W.
10. The method for preparing a botanical broad-spectrum antibacterial agent according to claim 6, wherein in step S03, the particle size of the stachys powder is 70 mesh, the ultrasonic temperature of the stachys powder is 90 ℃, and the ultrasonic power is 280W.
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Application publication date: 20210129 |