CN112251069A - 一种基于水性丝网印刷技术的酶电极用酶油墨及其制备方法 - Google Patents
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Abstract
本发明属于生物传感器技术领域，涉及一种基于水性丝网印刷技术的酶电极用酶油墨，该酶油墨包含以下重量百分比的组分：成膜剂1%~2%、增稠剂1.5%~17%、缓冲液55%~78%、酶稳定剂0.5%~1.5%、电子媒介体15%~30%、生物酶1.5%~3%、消泡剂0.5%~1.5%；所述成膜剂包括以下重量百分比的组分：聚乙烯醇7%~15%、水84.5%~92.5%、消泡剂0.5%~1.5%。该酶油墨能利用丝网印刷机直接印在基础电极上，减少了点酶机或涂布机的设备投入，生产成本低、重现性好、灵敏度高。
Description
技术领域
本发明属于生物传感器技术领域，涉及一种基于水性丝网印刷技术的酶电极用酶油墨及其制备方法。
背景技术
酶电极是最常用也是最早开发的生物传感器。酶电极是指电极敏感膜表面覆盖有一层很薄的含酶凝胶或悬浮液的离子选择电极，克服了游离酶的不稳定性，且能重复使用降低成本。酶是一类具有催化功能的活性物质，和化学催化剂相比，具有反应速度快、选择性好、反应条件温和、底物专一性强、可在水溶液和中性pH下操作等优点，同时酶本身可以被微生物降解，符合绿色化学的要求。但是游离酶也有对外界因素非常敏感容易失活、不易分离和纯化等问题。为了克服这些缺点，酶固定化技术应运而生。
酶的固定化是决定酶电极特性的关键技术。它既要保持活性物质酶本身的特性，又要避免游离酶应用上的缺陷。目前将酶固定于电极的方法有交联法、吸附法、包埋法、共价键合法、层层自组装技术。随着高精密度丝网印刷机在生物传感技术领域的推广和应用，酶电极基础电极的制备已能规模化生产，而且重现性好、灵敏度高。但是由于生物材料酶的特殊性，生物酶的丝网印刷还只能停留在实验室和中试阶段。这样导致生产厂家不得不在基础电极丝网印刷完成后，利用点酶或涂布技术将生物酶溶液点到或涂布到电极的表面。这样就需要额外添加点酶机或涂布机，使得生产成本增加，制作工艺复杂，而且产量低、重复性差，很难做到大规模连续化生产。如授权公告号为CN107238645B的发明专利公开了一种在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法，也是将配置好的生物酶悬浮液滴加于丝网印刷电极片上成固定化酶层。
发明内容
本发明的目的在于提供一种基于水性丝网印刷技术的酶电极用酶油墨及其制备方法，该酶油墨能利用丝网印刷机直接印在基础电极上，减少了点酶机或涂布机的设备投入，生产成本低、重现性好、灵敏度高。
为实现上述目的，本发明采用以下技术方案：
本发明提供一种基于水性丝网印刷技术的酶电极用酶油墨，包含以下重量百分比的组分：成膜剂1%~2%、增稠剂1.5%~17%、缓冲液55%~78%、酶稳定剂0.5%~1.5%、电子媒介体15%~30%、生物酶1.5%~3%、消泡剂0.5%~1.5%；所述成膜剂包括以下重量百分比的组分：聚乙烯醇7%~15%、水84.5%~92.5%、消泡剂0.5%~1.5%；所述消泡剂选自SF-104E、BYK-066或道康宁163中的一种或多种；所述增稠剂选自羟丙基甲基纤维素、羧甲基纤维素钠、甲基纤维素、羟丙基纤维素、羟乙基纤维素、明胶或水溶性纳米二氧化硅中的一种或多种；所述缓冲液包括以下重量百分比的组分：缓冲体系1%~2%、水98%~99%；所述酶稳定剂选自海藻糖、柠檬酸三钠或谷氨酸钠中的一种或多种；所述电子导电介体选自铁氰化钾或二茂铁甲酸。
优选地，所述成膜剂的制备方法包括以下步骤：取80-90℃的水搅拌下加入聚乙烯醇，保持恒温，待完全溶解后加入消泡剂，搅拌均匀。
优选地，所述成膜剂的旋转粘度为1300-6500mps，固含量为7%-15%。
优选地，所述缓冲液的pH值为5.5-6.8。
优选地，所述缓冲体系选自tris盐酸缓冲体系、磷酸盐缓冲体系、柠檬酸盐缓冲体系、琥珀酸盐缓冲体系、丁二酸盐缓冲体系或乙酸盐缓冲体系中的一种或多种。
优选地，所述酶电极用水性丝网印刷酶油墨的粘度为120000-160000mps，pH值为5.5-6.8，固含量为12~45%。
优选地，所述生物酶选自乳酸氧化酶、胆固醇氧化酶或葡萄糖氧化酶。
本发明还提供上述一种基于水性丝网印刷技术的酶电极用酶油墨的制备方法，包括以下步骤：取55%~78%的缓冲液加入0.5%-1.5%的酶稳定剂，充分搅拌溶解均匀后加入1.5%~17%的增稠剂，用1000-1800转/分转速研磨12-18小时后加入1%-2%的成膜剂、0.5%-1.5%的消泡剂，再研磨12个小时放入3℃冰箱，印刷前一小时内加入1.5%~3%的生物酶和15~30%的电子导电介体。
相比现有技术，本发明的有益效果在于：
本发明基于水性丝网印刷技术的酶电极用酶油墨稳定性好，能利用丝网印刷机直接印在基础电极上，减少了点酶机或涂布机的设备投入，可进行大规模生产，生产成本低、重现性好、灵敏度高，室温下干燥，产品组分无毒无害、不易燃爆、无挥发性有机气体。
附图说明
图1为本发明实施例1测得葡萄糖氧化酶电极的电流曲线。
图2为本发明实施例2测得乳酸氧化酶电极的电流曲线。
图3为本发明实施例3测得胆固醇氧化酶电极的电流曲线。
具体实施方式
以下实施例用于说明本发明，但不用来限定本发明的保护范围。若未特别指明，实施例中所用技术手段为本领域技术人员所熟知的常规手段。下述实施例中的试验方法，如无特别说明，均为常规方法。
以下实施例中所用水为二次蒸馏水，丝网印刷机型号为HS-6575M，生产厂家为东莞市大震丝印器材有限公司。
实施例一
先取85克的二次蒸馏水加热到80℃搅拌条件下加入7克的聚乙烯醇，保持恒温，待完全溶解后加入消泡剂BYK-066 0.2克，搅拌均匀后称重补加水至100克继续搅拌均匀得成膜剂，测其粘度为1300mps，放入3℃冰箱备用。
接着取60克的二次蒸馏水搅拌条件下加入酶稳定剂柠檬酸钠0.5克、谷氨酸钠0.5克充分溶解后加入磷酸盐缓冲体系使pH值达到6.8±0.05，1000转/分研磨条件下加入4克增稠剂羟丙基甲基纤维素，待胶液透明时加入1克的羟丙基纤维素和1克的羧甲基纤维素钠，研磨12个小时后放入3℃冰箱；8个小时后取出，放置室温，加入消泡剂SF-104E 0.5克、成膜剂2克，1000转/分条件下继续研磨12个小时后把水补够至73克，再搅拌4个小时后制得母液，放入3℃冰箱，备用。
利用水性丝网印刷技术进行酶电极大生产时，首先将母液放置到室温，测粘度为135000mps，加入1.5克的葡萄糖氧化酶和15克的铁氰化钾搅拌0.5小时，用丝网印刷机印在事先印制好的CV值合格的基础电极上，室温下固化30分钟后开始组装，得葡萄糖氧化酶电极。
将制好的测试条连接在uECS电化学工作站的工作电极和参比电极两端施加300mV的工作电压，分别测定0、30、50、100、200、300、500、600mg/dl浓度的葡萄糖，其电流曲线如图1所示。同生化分析仪，分别取60份葡萄糖质控液和60份糖尿病人血样葡萄糖对照测试，其相关性γ达到0.982，测试条的相对标准误差为CV≤4.5%。
实施例二
先取88克的二次蒸馏水加热到80℃搅拌条件下加入12克的聚乙烯醇，保持恒温，待完全溶解后加入消泡剂BYK-066 0.5克，搅拌均匀后称重补加水至100%继续搅拌均匀得成膜剂，测其粘度为5500mps，放入3℃冰箱备用。
接着取70克的二次蒸馏水搅拌条件下加入酶稳定剂柠檬酸钠0.5克、谷氨酸钠0.5克充分溶解后加入丁二酸盐缓冲体系使pH值达到6.8±0.05，1000转/分研磨条件下加入4%增稠剂甲基纤维素，待胶液透明时加入1克的羟乙基纤维素，研磨12个小时后放入3℃冰箱；8个小时后取出，放置室温，加入消泡剂道康宁0.5克、成膜剂1克，1000转/分条件下继续研磨12个小时后把水补够至72.5克，再搅拌4个小时后制得母液，放入3℃冰箱，备用。
利用水性丝网印刷技术进行酶电极大生产时，首先将母液放置到室温，测粘度为140000mps，加入20克的二茂铁甲酸，研磨12h后加入1克的乳酸氧化酶搅拌0.5小时，用丝网印刷机印在事先印制好的CV值合格的基础电极上，室温下固化30分钟后开始组装，得乳酸氧化酶电极。
将制好的测试条放在工作电极和参比电极两端施加300mV的工作电压，分别测定0、100、200、300、400、500、600（mg/dl）浓度的乳酸，其电流曲线如图2所示。同生化分析仪，分别取60份乳酸质控液和60份病人血样乳酸对照测试，其相关性γ达到0.952，测试条的相对标准误差为CV≤5.2%。
实施例三
先取85克的二次蒸馏水加热到80℃搅拌条件下加入15克的聚乙烯醇，保持恒温，待完全溶解后加入消泡剂BYK-066 0.5克，搅拌均匀后称重补加水至100%继续搅拌均匀得成膜剂，测其粘度为6500mps，放入3℃冰箱备用。
接着取60克的二次蒸馏水搅拌条件下加入酶稳定剂海藻糖0.5克、谷氨酸钠0.5克充分溶解后加入柠檬酸盐缓冲体系使pH值达到6.7±0.05，1000转/分研磨条件下加入1%增稠剂羟乙基纤维素，待胶液透明时加入4克的亲水性纳米二氧化硅，研磨12个小时后放入3℃冰箱；8个小时后取出，放置室温，加入消泡剂SF-104E 0.5克、成膜剂1克，1000转/分条件下继续研磨12个小时后把水补够至62克，再搅拌4个小时后制得母液，放入3℃冰箱，备用。
利用水性丝网印刷技术进行酶电极大生产时，首先将母液放置到室温，测粘度为135000mps，加入1.2克的胆固醇氧化酶和30克的铁氰化钾搅拌0.5小时，用丝网印刷机印在事先印制好的CV值合格的基础电极上，室温下固化30分钟后开始组装，得胆固醇氧化酶电极。
将制好的测试条放在工作电极和参比电极两端施加300mV的工作电压，分别测定0、100、200、300、400、500、600（mg/dl）浓度的胆固醇，其电流曲线如图3所示。同生化分析仪，分别取40份胆固醇质控液和40份病人血样胆固醇对照测试，其相关性γ达到0.973，测试条的相对标准误差为CV≤3.8%。
以上所述之实施例，只是本发明的较佳实施例而已，仅仅用以解释本发明，并非限制本发明实施范围，对于本技术领域的技术人员来说，当然可根据本说明书中所公开的技术内容，通过置换或改变的方式轻易做出其它的实施方式，故凡在本发明的原理上所作的变化和改进等，均应包括于本发明申请专利范围内。
Claims (8)
1.一种基于水性丝网印刷技术的酶电极用酶油墨，其特征在于，包含以下重量百分比的组分：成膜剂1%~2%、增稠剂1.5%~17%、缓冲液55%~78%、酶稳定剂0.5%~1.5%、电子媒介体15%~30%、生物酶1.5%~3%、消泡剂0.5%~1.5%；所述成膜剂包括以下重量百分比的组分：聚乙烯醇7%~15%、水84.5%~92.5%、消泡剂0.5%~1.5%；所述消泡剂选自SF-104E、BYK-066或道康宁163中的一种或多种；所述增稠剂选自羟丙基甲基纤维素、羧甲基纤维素钠、甲基纤维素、羟丙基纤维素、羟乙基纤维素、明胶或水溶性纳米二氧化硅中的一种或多种；所述缓冲液包括以下重量百分比的组分：缓冲体系1%~2%、水98%~99%；所述酶稳定剂选自海藻糖、柠檬酸三钠或谷氨酸钠中的一种或多种；所述电子导电介体选自铁氰化钾或二茂铁甲酸。
2.根据权利要求1所述的一种基于水性丝网印刷技术的酶电极用酶油墨，其特征在于，所述成膜剂的制备方法包括以下步骤：取80-90℃的水搅拌下加入聚乙烯醇，保持恒温，待完全溶解后加入消泡剂，搅拌均匀。
3.根据权利要求1或2所述的一种基于水性丝网印刷技术的酶电极用酶油墨，其特征在于，所述成膜剂的旋转粘度为1300-6500mps，固含量为7%-15%。
4.根据权利要求1所述的一种基于水性丝网印刷技术的酶电极用酶油墨，其特征在于，所述缓冲液的pH值为5.5-6.8。
5.根据权利要求1所述的一种基于水性丝网印刷技术的酶电极用酶油墨，其特征在于，所述缓冲体系选自tris盐酸缓冲体系、磷酸盐缓冲体系、柠檬酸盐缓冲体系、琥珀酸盐缓冲体系、丁二酸盐缓冲体系或乙酸盐缓冲体系中的一种或多种。
6.根据权利要求1所述的一种基于水性丝网印刷技术的酶电极用酶油墨，其特征在于，所述酶电极用水性丝网印刷酶油墨的粘度为120000-160000mps，pH值为5.5-6.8，固含量为12~45%。
7.根据权利要求1所述的一种基于水性丝网印刷技术的酶电极用酶油墨，其特征在于，所述生物酶选自乳酸氧化酶、胆固醇氧化酶或葡萄糖氧化酶。
8.权利要求1~7任一项所述的一种基于水性丝网印刷技术的酶电极用酶油墨的制备方法，其特征在于，包括以下步骤：取55%~78%的缓冲液加入0.5%-1.5%的酶稳定剂，充分搅拌溶解均匀后加入1.5%~17%的增稠剂，用1000-1800转/分转速研磨12-18小时后加入1%-2%的成膜剂、0.5%-1.5%的消泡剂，再研磨12个小时放入3℃冰箱，印刷前一小时内加入1.5%~3%的生物酶和15~30%的电子导电介体。
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