CN112246292A - Microfluid implementation device for controlling gas-liquid linkage - Google Patents
Microfluid implementation device for controlling gas-liquid linkage Download PDFInfo
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- CN112246292A CN112246292A CN202011166819.7A CN202011166819A CN112246292A CN 112246292 A CN112246292 A CN 112246292A CN 202011166819 A CN202011166819 A CN 202011166819A CN 112246292 A CN112246292 A CN 112246292A
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- 239000007788 liquid Substances 0.000 title claims abstract description 135
- 239000000872 buffer Substances 0.000 claims abstract description 80
- 239000007853 buffer solution Substances 0.000 claims abstract description 43
- 230000001681 protective effect Effects 0.000 claims abstract description 35
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 239000002699 waste material Substances 0.000 claims description 41
- 229910000831 Steel Inorganic materials 0.000 claims description 26
- 239000010959 steel Substances 0.000 claims description 26
- 230000000149 penetrating effect Effects 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 10
- 102100039856 Histone H1.1 Human genes 0.000 claims description 7
- 102100039855 Histone H1.2 Human genes 0.000 claims description 7
- 101001035402 Homo sapiens Histone H1.1 Proteins 0.000 claims description 7
- 101001035375 Homo sapiens Histone H1.2 Proteins 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 3
- 238000009434 installation Methods 0.000 abstract description 3
- 238000004026 adhesive bonding Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention provides a microfluid implementation device for controlling gas-liquid linkage, which comprises an upper cover, an internal structure and a protective cover, wherein the internal structure comprises a main deck, a chip bracket, a microfluidic chip, a container, a multifunctional joint, a micro hose and a micro hard tube, the chip bracket is fixed on the main deck, and the microfluidic chip is fixed on the chip bracket; the container comprises a sample container and a buffer solution container, and a sample container cover and a buffer solution container cover are respectively arranged on the sample container and the buffer solution container; the multifunctional joint is arranged on the sample container cover and the buffer liquid container cover; the upper cover is fixed on the main deck; the Microsoft pipe is connected with the multifunctional joint and/or the microfluidic chip; the protective cover encases the sample container and the buffer container. The invention has convenient installation, no need of gluing and good sealing property.
Description
Technical Field
The invention belongs to the technical field of microfluid, and particularly relates to a microfluid implementation device for controlling gas-liquid linkage.
Background
In scientific experiments of biology, chemistry, materials and the like, fluid operation is often required, and the microfluidic biochip is greatly valued at present because of its advantages of light volume, small amount of used samples/reagents, fast reaction speed, massive parallel processing, disposability and the like, so that the application range in biotechnology research is very wide. The technical core of the micro-fluid control device is the conversion from a macroscopic centimeter-level pipeline to a micron-level microscopic pipeline, so that gas-liquid linkage is realized, and the air tightness of the micro-fluid control device is ensured. The difficulty of the prior art is that the conversion from the macroscopic centimeter-level pipeline to the micron-level microscopic pipeline is realized, and the air tightness is kept good in the pipeline conversion process. At present, the existing pipeline and the pipeline on the market are connected in a mode that the pipeline and the container are connected by a common clamping pipe joint, the clamping pipe joint easily causes locking phenomena to the soft and fine pipelines, or seals the joint by a glue applying mode, but the glue sealing mode is not ideal for the air tightness of the pipelines, and the phenomena of air leakage and liquid leakage still exist.
Disclosure of Invention
The invention aims to provide a microfluid implementation device for controlling gas-liquid linkage, so as to ensure the conversion from a macroscopic centimeter-level pipeline to a micron-level microscopic pipeline and ensure the air tightness of the pipeline.
In order to achieve the purpose, the invention provides a micro-fluid realizing device for controlling gas-liquid linkage, which comprises an upper cover, an internal structure and a protective cover, and is characterized in that the internal structure comprises a main deck, a chip bracket, a micro-fluidic chip, a container, a multifunctional joint, a micro-hose and a micro-hard tube, wherein the chip bracket is fixed on the main deck, and the micro-fluidic chip is fixed on the chip bracket; the container comprises a sample container and a buffer solution container, wherein the sample container and the buffer solution container are both cylinders, the sample container and the buffer solution container are respectively provided with a sample container cover and a buffer solution container cover, the sample container and the sample container cover are fixed in a threaded screwing mode, and the buffer solution container cover are fixed in a threaded screwing mode; the multifunctional joint is arranged on the sample container cover and the buffer container cover, and the multifunctional joint and the sample container cover and the buffer container cover realize the sealing performance in an interference fit mode; the micro-hard tube is inserted into the multifunctional joint and penetrates into the sample container cover and the buffer container cover; the upper cover is fixed on the main deck and used for protecting the micro-fluidic chip and the micro-hose; the Microsoft pipe is connected with the multifunctional joint and/or the microfluidic chip; the protective cover is sleeved on the sample container and the buffer container, and the protective cover is fixed on the main deck and used for protecting the sample container and the buffer container.
Furthermore, the microfluidic chip is provided with four interfaces, namely a sample inlet, a buffer solution inlet, an enriched solution outlet and a waste solution outlet, wherein the sample inlet and the buffer solution inlet are positioned at one end of the microfluidic chip, and the enriched solution outlet and the waste solution outlet are positioned at the other end of the microfluidic chip.
Furthermore, the micro hose comprises a sample air inlet pipe, a sample inlet pipe, a buffer solution air inlet pipe and a buffer solution inlet pipe; the micro hose is connected with the micro-fluidic chip, the micro hose is connected with the multifunctional joint through the steel needle, and the micro hose, the micro-fluidic chip and the multifunctional joint are connected to form a sealed micro pipeline
Further, the main deck is runway-shaped, and including two straight flanges, first straight flange and second straight flange, all be equipped with four poling holes on two straight flanges, the poling hole that first straight flange was equipped with is in proper order for advancing appearance pipe poling hole entry, advance appearance pipe poling hole export, feed liquor pipe poling hole entry, feed liquor pipe poling hole export, be equipped with the poling hole on the second straight flange and be enrichment liquid pipe poling hole entry, enrichment liquid pipe poling hole export, waste liquid pipe poling hole entry, waste liquid pipe poling hole export in proper order.
Furthermore, the enrichment liquid discharge pipe and the waste liquid discharge pipe are arranged on the main deck, a pipe clamping valve position is further arranged on the outer wall of the main deck and comprises a sample inlet pipe clamping valve position, a buffer liquid inlet pipe clamping valve position, an enrichment liquid pipe clamping valve position F3 and a waste liquid pipe clamping valve position, the sample inlet pipe clamping valve position and the buffer liquid inlet pipe clamping valve position are arranged on the outer wall of the first straight edge, and the enrichment liquid pipe clamping valve position and the waste liquid pipe clamping valve position are arranged on the outer wall of the second straight edge; the main deck still is equipped with deck locating pin and deck buckle, the protection casing is equipped with the protection casing constant head tank with the protection casing draw-in groove, the deck locating pin with protection casing constant head tank 302 corresponds, the deck buckle with the protection casing draw-in groove corresponds.
Further, the container cover comprises a sample container cover and a buffer liquid container cover, and an O-shaped sealing ring is arranged between the container cover and the container.
Further, the multifunctional joint comprises a first multifunctional joint and a second multifunctional joint, the first multifunctional joint is arranged on the sample container cover, the second multifunctional joint is arranged on the buffer container cover, the first multifunctional joint comprises a first joint and a second joint, the second multifunctional joint comprises a third joint and a fourth joint, the first joint and the fourth joint are respectively inserted from the center points of the sample container cover and the buffer container cover, the second joint is connected with the first joint side by side and inserted into the sample container cover, and the third joint and the fourth joint are connected with each other side by side and inserted into the buffer container cover.
Further, the micro-hard tube is used for contacting liquid, and the liquid in the container enters the micro-hose through the micro-hard tube.
Furthermore, the upper cover is provided with four outlet openings, namely a sample air inlet pipe outlet opening, a buffer air inlet pipe outlet opening, a concentrated liquid discharge pipe outlet opening and a waste liquid discharge pipe outlet opening.
The assembly method of the microfluid realization device for controlling gas-liquid linkage is characterized by comprising the following steps:
step 2, placing the first joint and the second joint into a sample container cover, and placing the second joint and the third joint into a buffer container cover;
step 3, two sets of micro-hard tubes are provided, one set of micro-hard tubes is inserted into the first joint from the inner side of the sample container cover, and one set of micro-hard tubes is inserted into the fourth joint from the inner side of the buffer container cover;
step 4, adding an O-shaped sealing ring into the sample container cover and the sample container, assembling the sample container cover and the sample container together, screwing the sample container cover and the sample container, and then assembling the sample container and the sample container into a main deck;
step 5, adding an O-shaped sealing ring into a buffer container cover and the buffer container, assembling the buffer container cover and the buffer container together, screwing the buffer container and the buffer container, and then assembling the buffer container and the buffer container into a main deck;
step 6, inserting a steel needle into one end of the sample air inlet pipe, and then inserting one end with the steel needle into a second joint;
step 7, inserting a steel needle into one end of the buffer solution air inlet pipe, and then inserting one end with the steel needle into a third connector;
step 8, inserting two steel needles into two ends of a sample inlet pipe respectively, inserting one end of the sample inlet pipe into a sample inlet of the microfluidic chip, inserting the other end of the sample inlet pipe into a sample inlet hole of the main deck, penetrating the other end of the sample inlet pipe out of a sample inlet pipe penetrating hole outlet, and inserting the sample inlet pipe into the first connector;
step 9, inserting two steel needles into two ends of a buffer liquid inlet pipe respectively, inserting one end of the buffer liquid inlet pipe into a sample inlet of the microfluidic chip, inserting the other end of the buffer liquid inlet pipe into a liquid inlet pipe through pipe hole inlet of the main deck, penetrating out of a liquid inlet pipe through pipe hole outlet, and inserting the liquid inlet pipe into a fourth joint;
step 10, enabling a sample air inlet pipe to penetrate out of a sample air inlet pipe outlet pipe opening of the upper cover, enabling a buffer liquid air inlet pipe to penetrate out of a buffer liquid air inlet pipe outlet pipe opening, enabling a concentrated liquid discharge pipe to penetrate out of a concentrated liquid discharge pipe outlet pipe opening, enabling a waste liquid discharge pipe to penetrate out of a waste liquid discharge pipe outlet pipe opening, and then assembling the upper cover and the main deck together;
step 11, mounting the protective cover on the main deck, wherein the protective cover can be placed in place only when the main deck positioning pin is aligned with the protective cover positioning groove, and the deck buckle on the main deck is clamped in the protective cover clamping groove of the protective cover after the protective cover is placed in place so as to achieve the effect of fixing the protective cover;
step 12, when the microfluid device for controlling gas-liquid linkage is installed on a corresponding instrument, the sample injection tube clamping valve position is used for controlling the on-off state of the sample injection tube; the buffer solution inlet pipe clamping valve position is used for controlling the on-off state of the buffer solution inlet pipe; the rich liquid pipe clamping valve position is used for controlling the on-off state of the rich liquid discharge pipe; the waste liquid discharge pipe clamping valve position is used for controlling the on-off state of the waste liquid discharge pipe.
Compared with the prior art, the invention has the beneficial effects that: the micro-hose is connected with the multifunctional joint or the micro-fluidic chip through the steel needle, the steel needle can be directly inserted, the phenomenon of pipeline locking caused by using a common pipe clamping joint is avoided, the installation is convenient, glue is not required to be applied, and the sealing performance is good; the multifunctional joint is connected with the container cover by direct insertion, the installation is convenient, no glue is required to be applied, the liquid flow between the micro hose and the container is ensured, and the sealing property is good; the sealing mode of the container cover and the container is fixed in a thread tightening mode through a special O-shaped sealing ring, the sealing performance of the container is guaranteed, and the phenomena of air leakage and liquid leakage are avoided.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a top view of the internal structure of the present invention;
FIG. 3 is a schematic view of the assembly of a first multi-functional adaptor, a sample container cover, and a sample container of the present invention;
FIG. 4 is an assembled rear cross-sectional view of a first multi-functional adaptor, a sample container lid and a sample container of the present invention;
FIG. 5 is a schematic view of the steel needle connection of the present invention.
Reference numerals:
in fig. 1:
in fig. 2:
reference numerals | Name of component | Reference numerals | Name of component |
A | Sampling tube through-tube hole inlet | H1-1 | Sample inlet |
B | Sampling tube through-tube hole outlet | H1-2 | Buffer inlet |
C | Liquid inlet pipe penetrating hole inlet | H2-1 | Outlet of enriched liquid |
D | Liquid inlet pipe through pipe hole outlet | H2-2 | Waste liquid outlet |
E | Pipe hole inlet of enrichment liquid pipe | F1 | Sample inlet pipe clamping valve position |
F | Outlet of pipe hole for enriching liquid | F2 | Buffer liquid inlet pipe clamping valve position |
H | Waste liquid pipe through pipe hole inlet | F3 | Rich liquid pipe clamping valve position |
I | Waste liquid pipe through hole outlet | F4 | Waste liquid pipe clamping valve position |
In fig. 3: 210. an O-shaped sealing ring; 211. a micro-hard tube;
in fig. 4: g0, steel needle.
Detailed Description
The following provides a more detailed description of embodiments of the invention, as illustrated in the accompanying drawings.
The invention provides a microfluid implementation device for controlling gas-liquid linkage, which comprises an upper cover 201, an internal structure and a protective cover 214, wherein the internal structure comprises a main deck 203, a chip bracket 202, a microfluidic chip H0, a container, a multifunctional joint, a micro hose and a micro hard tube 211, the chip bracket 202 is fixed on the main deck 203, and the microfluidic chip H0 is fixed on the chip bracket 202; the container comprises a sample container 212 and a buffer solution container 213, both the sample container 212 and the buffer solution container 213 are cylinders, a sample container cover 208 and a buffer solution container cover 209 are respectively arranged on the sample container 212 and the buffer solution container 213, the sample container 212 and the sample container cover 208 are fixed in a thread screwing mode, and the buffer solution container 213 and the buffer solution container cover 209 are fixed in a thread screwing mode; the multifunctional joint is arranged on the sample container cover 208 and the buffer container cover 209, and the multifunctional joint and the sample container cover 208 and the buffer container cover 209 realize the sealing performance in an interference fit mode; a micro-tube 211 is inserted into the multi-functional adapter and threaded into the sample container lid 208 and buffer container lid 209; the upper cover 201 is fixed on the main deck 203 and used for protecting the microfluidic chip H0 and the micro hose; the Microsoft tube is connected with the multifunctional joint and/or the microfluidic chip H0; a shield 214 surrounds the sample container 212 and the buffer container 213, the shield 214 being secured to the main deck 203 for protecting the sample container 212 and the buffer container 213.
The microfluidic chip H0 is provided with four interfaces, a sample inlet H1-1, a buffer solution inlet H1-2, an enrichment solution outlet H2-1 and a waste liquid outlet H2-2 are arranged at one end of the microfluidic chip, a sample inlet H1-1 and a buffer solution inlet H1-2 are arranged at the other end of the microfluidic chip, and the enrichment solution outlet H2-1 and the waste liquid outlet H2-2 are arranged at the other end of the microfluidic chip.
The Microsoft pipe comprises a sample inlet pipe 101, a sample inlet pipe 105, a buffer solution inlet pipe 102 and a buffer solution inlet pipe 106; the micro-hose is connected with the micro-fluidic chip H0, the Microsoft tube and the multifunctional joint through a steel needle G0, and the three are connected to form a sealed micro-pipeline.
The main deck 203 is runway-shaped, and includes two straight sides, first straight side and second straight side, and two straight sides respectively are equipped with four poling holes, and the poling hole that first straight side was equipped with is in proper order for advance appearance pipe poling hole entry A, advance appearance pipe poling hole export B, feed liquor pipe poling hole entry C, feed liquor pipe poling hole export D, be equipped with the poling hole on the second straight side and be enrichment liquid pipe poling hole entry E, enrichment liquid pipe poling hole export F, waste liquid pipe poling hole entry G, waste liquid pipe poling hole export H in proper order.
The enrichment pipe is provided with an enrichment liquid discharge pipe 103 and a waste liquid discharge pipe 104, the outer wall of the main deck 203 is also provided with a pipe clamping valve position which comprises a sampling pipe clamping valve position F1, a buffer liquid inlet pipe clamping valve position F2, an enrichment pipe clamping pipe valve position F3 and a waste liquid clamping pipe valve position F4, the sampling pipe clamping valve position F1 and the buffer liquid inlet pipe clamping valve position F2 are arranged on the outer wall of the first straight edge, and the enrichment pipe clamping pipe valve position F3 and the waste liquid clamping pipe valve position F4 are arranged on the outer wall of the second straight edge; the main deck 203 is further provided with a deck positioning pin 301 and a deck buckle 303, the shield 214 is provided with a shield positioning slot 302 and a shield clamping slot 304, the deck positioning pin 301 corresponds to the shield positioning slot 302, and the deck buckle 303 corresponds to the shield clamping slot 304.
The container covers, including the sample container cover 208 and the buffer container cover 209, have an O-ring seal 210 mounted between them.
The multifunctional joint comprises a first multifunctional joint and a second multifunctional joint, the first multifunctional joint is arranged on the sample container cover, the second multifunctional joint is arranged on the buffer container cover, the first multifunctional joint comprises a first joint 204 and a second joint 205, the second multifunctional joint comprises a third joint 206 and a fourth joint 207, the first joint 204 and the fourth joint 207 are respectively inserted from the center points of the sample container cover 208 and the buffer container cover 209, the second joint 205 is connected with the first joint 204 side by side and inserted into the sample container cover 208, and the third joint 206 is connected with the fourth joint 207 side by side and inserted into the buffer container cover 209.
The micro-rigid tube is used to contact the liquid, and the liquid in the container enters the micro-flexible tube through the micro-rigid tube 211.
The upper cover is provided with four outlet orifices, namely a sample air inlet pipe outlet orifice A0, a buffer air inlet pipe outlet orifice B0, a concentrated liquid discharge pipe outlet orifice C0 and a waste liquid discharge pipe outlet orifice D0.
As shown in fig. 1 to 3, the present invention provides an assembly method of a microfluidic device for controlling gas-liquid linkage, which comprises the following steps:
step 2, loading the first adapter 204 and the second adapter 205 into a sample container cover 208, and loading the second adapter 206 and the third adapter 207 into a buffer container cover 209;
step 3, two sets of micro-hard tubes 211 are provided, wherein one set of micro-hard tubes 211 are inserted into the first joint 204 from the inner side of the sample container cover 208, and one set of micro-hard tubes 211 are inserted into the fourth joint 207 from the inner side of the buffer container cover 209;
step 4, adding an O-shaped sealing ring 210 between the sample container cover 208 and the sample container 212, assembling and screwing the O-shaped sealing ring together, and then assembling the O-shaped sealing ring into the main deck 203;
step 5, adding an O-shaped sealing ring 210 between a buffer container cover 209 and a buffer container 213, assembling together and screwing, and then assembling into the main deck 203;
step 6, inserting a steel needle G0 into one end of the sample air inlet pipe 101, and then inserting one end with a steel needle G0 into the second connector 205;
step 7, inserting a steel needle G0 into one end of the buffer solution inlet pipe 102, and then inserting one end with a steel needle G0 into the third connector 206;
step 8, inserting two steel needles G0 into two ends of the sample inlet tube 104, respectively, inserting one end of the sample inlet tube into a sample inlet H1-1 of the microfluidic chip, inserting the other end of the sample inlet tube into a sample inlet tube through hole inlet a of the main deck, penetrating the other end of the sample inlet tube through hole outlet B of the main deck, and then inserting the sample inlet tube into the first connector 204;
step 9, inserting two steel needles G0 into two ends of the buffer liquid inlet pipe 105 respectively, inserting one end of the buffer liquid inlet pipe into a sample inlet H1-2 of the microfluidic chip, inserting the other end of the buffer liquid inlet pipe into a liquid inlet pipe through pipe hole inlet C of the main deck, penetrating the other end of the buffer liquid inlet pipe through pipe hole outlet D of the main deck, and then inserting the other end of the buffer liquid inlet pipe into the fourth joint 207;
step 10, penetrating a sample air inlet pipe out of a sample air inlet pipe outlet opening A0 of an upper cover 201, penetrating a buffer air inlet pipe 102 out of a buffer liquid air inlet pipe outlet opening B0, penetrating a concentrated liquid discharge pipe 103 out of a concentrated liquid discharge pipe outlet opening C0, penetrating a waste liquid discharge pipe 104 out of a waste liquid discharge pipe outlet opening D0, and then assembling the upper cover 201 and a main deck 203 together;
step 11, mounting the protective cover 214 on the main deck 203, wherein the protective cover can be placed in place only when the main deck positioning pin 301 is aligned with the protective cover positioning groove 302, and the deck buckle 303 on the main deck is clamped in the protective cover clamping groove 304 of the protective cover after the protective cover is placed in place so as to achieve the effect of fixing the protective cover;
step 12, when the microfluidic device for controlling gas-liquid linkage is installed on a corresponding instrument, the sample injection tube clamping valve position F1 is used for controlling the on-off state of the sample injection tube 105; the buffer solution inlet pipe clamping valve position F2 is used for controlling the on and off state of the buffer solution inlet pipe 106; the rich liquid pipe clamping valve position F3 is used for controlling the on and off states of the rich liquid discharge pipe 103; the waste liquid discharge pipe pinching valve position F4 is used to control the on and off state of the waste liquid discharge pipe 105. The buffer container lid 209, the buffer container 213, and the second multi-functional joint are assembled similarly to the sample container lid 208, the sample container 212, and the first multi-functional joint, as shown in fig. 3-4.
For better explanation, the invention is proved by experiments that the sealing performance is good, and the specific examples for time verification are as follows:
the experimental method comprises the following steps: connecting the concentrated solution discharge pipe 209 and the waste solution discharge pipe 210 by using a steel needle G0, driving a cylinder to simultaneously add 2000mBar pressure from the sample gas inlet pipe 101 and the buffer solution gas inlet pipe 102 by using a stepping motor, maintaining for 2min, recording a pressure value and the position of the stepping motor every 20s, observing the pressure and the step number change condition of the stepping motor, and measuring the following experimental data:
from three sets of experimental data: under the condition that the pressure is kept stable, the step number of the stepping motor is kept constant within a period of time and does not change, and the sealing performance is good in the implementation process, and the phenomena of liquid leakage and air leakage do not exist.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A microfluid implementation device for controlling gas-liquid linkage comprises an upper cover (201), an internal structure and a protective cover (214), and is characterized in that the internal structure comprises a main deck (203), a chip support (202), a microfluidic chip (H0), a container, a multifunctional joint, a micro hose and a micro hard tube (211), the chip support (202) is fixed on the main deck (203), and the microfluidic chip (H0) is fixed on the chip support (202); the container comprises a sample container (212) and a buffer solution container (213), the sample container (212) and the buffer solution container (213) are both cylinders, a sample container cover (208) and a buffer solution container cover (209) are respectively arranged on the sample container (212) and the buffer solution container (213), the sample container (212) and the sample container cover (208) are fixed in a threaded screwing mode, and the buffer solution container (213) and the buffer solution container cover (209) are fixed in a threaded screwing mode; the multifunctional joint is arranged on the sample container cover (208) and the buffer container cover (209), and the multifunctional joint and the sample container cover (208) and the buffer container cover (209) realize sealing performance in an interference fit mode; the micro-tube (211) is inserted into the multi-functional adapter and penetrates into the sample container lid (208) and buffer container lid (209); the upper cover (201) is fixed on the main deck (203) and is used for protecting the microfluidic chip (H0) and the micro hose; the microsoft tube is connected with the multifunctional joint and/or the microfluidic chip (H0); the protective cover (214) is used for covering the sample container (212) and the buffer container (213), and the protective cover (214) is fixed on the main deck (203) and used for protecting the sample container (212) and the buffer container (213).
2. The device for realizing microfluid of a control gas-liquid linkage of claim 1, wherein the microfluidic chip is provided with four interfaces, a sample inlet (H1-1), a buffer inlet (H1-2), an enriched liquid outlet (H2-1), and a waste liquid outlet (H2-2), wherein the sample inlet (H1-1) and the buffer inlet (H1-2) are located at one end of the microfluidic chip, and the enriched liquid outlet (H2-1) and the waste liquid outlet (H2-2) are located at the other end of the microfluidic chip.
3. The microfluidic implementation device for controlling gas-liquid linkage according to claim 1, wherein the micro hose comprises a sample inlet pipe (101), a sample inlet pipe (105), a buffer inlet pipe (102), and a buffer inlet pipe (106); the micro-hose is connected with the micro-fluidic chip (H0) and the multi-functional joint through the steel needle (G0), and the three are connected to form a sealed micro-pipeline.
4. The device for realizing the microfluid of the control gas-liquid linkage of claim 1, wherein the main deck (203) is racetrack-shaped, and comprises two straight sides, a first straight side and a second straight side, wherein the two straight sides are respectively provided with four through holes, the through holes arranged on the first straight side are sequentially an inlet (A) of a sample inlet tube through hole, an outlet (B) of the sample inlet tube through hole, an inlet (C) of a liquid inlet tube through hole and an outlet (D) of the liquid inlet tube through hole, and the through holes arranged on the second straight side are sequentially an inlet (E) of an enrichment liquid tube through hole, an outlet (F) of the enrichment liquid tube through hole, an inlet (G) of a waste liquid tube through hole and an outlet (H) of the waste liquid tube through hole.
5. The microfluid implementation device according to claim 1, wherein the main deck is provided with an enrichment liquid discharge pipe (103) and a waste liquid discharge pipe (104), the outer wall of the main deck (203) is further provided with a tube clamping valve position, which includes a sample inlet tube clamping valve position (F1), a buffer liquid inlet tube clamping valve position (F2), an enrichment tube clamping tube valve position (F3), and a waste liquid clamping tube valve position (F4), the sample inlet tube clamping valve position (F1) and the buffer liquid inlet tube clamping valve position (F2) are disposed on the outer wall of the first straight side, and the enrichment tube clamping tube valve position (F3) and the waste liquid clamping tube valve position (F4) are disposed on the outer wall of the second straight side; the main deck (203) is further provided with a deck positioning pin (301) and a deck buckle (303), the protective cover (214) is provided with the protective cover positioning groove (302) and the protective cover clamping groove (304), the deck positioning pin (301) corresponds to the protective cover positioning groove (302), and the deck buckle (303) corresponds to the protective cover clamping groove (304).
6. The microfluidic device according to claim 1, wherein the container cover comprises a sample container cover (208) and a buffer container cover (209), and an O-ring (210) is installed between the container cover and the container.
7. The micro-fluid implementation device for controlling gas-liquid linkage according to claim 1, the multi-function adapter comprises a first multi-function adapter and a second multi-function adapter, the first multi-function adapter is mounted on the sample container cover, the second multi-functional adapter is mounted on the buffer container cap, the first multi-functional adapter comprises a first adapter (204) and a second adapter (205), the second multi-function connector comprises a third connector (206) and a fourth connector (207), said first connector (204) and said fourth connector (207) are inserted from the container lid centre points of the sample container lid (208) and buffer container lid (209), respectively, the second adapter (205) is connected side by side with the first adapter (204) and inserted into the sample container lid (208), the third joint (206) and the fourth joint (207) are connected side by side and inserted into the buffer container cover (209).
8. A microfluidic device for controlling gas-liquid linkage according to claim 1, wherein the micropipe is used to contact liquid, and liquid in the container enters the micropipe through the micropipe (211).
9. The microfluid implementation device of claim 1, wherein the top cover has four outlets, namely a sample inlet pipe outlet (a0), a buffer inlet pipe outlet (B0), a concentrated solution discharge pipe outlet (C0), and a waste solution discharge pipe outlet (D0).
10. A method of assembling a microfluidic device for controlling gas-liquid linkage according to any one of claims 1 to 9, comprising the steps of:
step 1, fixing a microfluidic chip (H0) on a chip bracket (202), and then fixing the chip bracket (202) on a main deck (203);
step 2, loading a first joint (204) and a second joint (205) into a sample container cover (208), and loading a second joint (206) and a third joint (207) into a buffer container cover (209);
step 3, two sets of micro-hard tubes (211) are provided, one set of micro-hard tubes (211) is inserted into the first joint (204) from the inner side of the sample container cover (208), and one set of micro-hard tubes (211) is inserted into the fourth joint (207) from the inner side of the buffer container cover (209);
step 4, a sample container cover (208) and a sample container (212) are assembled together by adding an O-shaped sealing ring (210) and are screwed tightly, and then the sample container cover and the sample container are assembled into a main deck (203);
step 5, adding an O-shaped sealing ring (210) into a buffer container cover (209) and a buffer container (213) to be assembled together and screwed, and then assembling the buffer container cover and the buffer container into a main deck (203);
step 6, inserting a steel needle (G0) into one end of the sample air inlet pipe (101), and then inserting one end with the steel needle (G0) into the second connector (205);
step 7, inserting a steel needle (G0) into one end of the buffer solution air inlet pipe (102), and then inserting one end with the steel needle (G0) into the third connector (206);
step 8, inserting two steel needles (G0) into two ends of a sample inlet tube (104) respectively, inserting one end of the sample inlet tube into a sample inlet (H1-1) of the microfluidic chip, inserting the other end of the sample inlet tube into a sample inlet tube hole inlet (A) of the main deck, penetrating the other end of the sample inlet tube out of a sample inlet tube hole outlet (B), and then inserting the sample inlet tube into a first connector (204);
step 9, inserting two steel needles (G0) into two ends of a buffer solution liquid inlet pipe (105) respectively, inserting one end of the buffer solution liquid inlet pipe into a sample inlet (H1-2) of the microfluidic chip, inserting the other end of the buffer solution liquid inlet pipe into a liquid inlet pipe through pipe hole inlet (C) of the main deck, penetrating the other end of the buffer solution liquid inlet pipe out of a liquid inlet pipe through pipe hole outlet (D), and then inserting the other end of the buffer solution liquid inlet pipe into a fourth connector (207);
step 10, enabling a sample air inlet pipe to penetrate out of a sample air inlet pipe outlet (A0) of an upper cover (201), enabling a buffer liquid air inlet pipe (102) to penetrate out of a buffer liquid air inlet pipe outlet (B0), enabling a concentrated liquid discharge pipe (103) to penetrate out of a concentrated liquid discharge pipe outlet (C0), enabling a waste liquid discharge pipe (104) to penetrate out of a waste liquid discharge pipe outlet (D0), and then assembling the upper cover (201) and a main deck (203) together;
step 11, mounting the protective cover (214) on the main deck (203), wherein the protective cover can be placed in place only when the main deck positioning pin (301) is aligned with the protective cover positioning groove (302), and the deck buckle (303) on the main deck is clamped in the protective cover clamping groove (304) of the protective cover after the protective cover is placed in place so as to achieve the effect of fixing the protective cover;
step 12, when the microfluidic device for controlling gas-liquid linkage is installed on a corresponding instrument, a sample inlet pipe clamping valve position (F1) is used for controlling the on-off state of a sample inlet pipe (105); the buffer solution inlet pipe clamping valve position (F2) is used for controlling the on-off state of the buffer solution inlet pipe (106); the rich liquid pipe clamping valve position (F3) is used for controlling the on-off state of the rich liquid discharge pipe (103); the waste liquid discharge pipe clamping valve position (F4) is used for controlling the on and off states of the waste liquid discharge pipe (105).
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