CN112221546A - Sample transfer device, transfer system and method - Google Patents
Sample transfer device, transfer system and method Download PDFInfo
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- CN112221546A CN112221546A CN202010880487.2A CN202010880487A CN112221546A CN 112221546 A CN112221546 A CN 112221546A CN 202010880487 A CN202010880487 A CN 202010880487A CN 112221546 A CN112221546 A CN 112221546A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
- B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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Abstract
The invention relates to the technical field of nucleic acid sample detection, in particular to a sample transfer device and a sample introduction system. The method comprises the following steps: the sample rack is provided with at least two mounting channels, and the mounting channels are used for mounting sample tubes with pierceable first sealing films on the bottoms in a one-to-one correspondence manner; a first lancing mechanism having at least two first lancing portions on the bottom of the sample rack, at least one first lancing portion corresponding to one of the mounting channels; the first driving part can be connected with one of the sample rack and the first puncture mechanism, and is driven by the driving force to drive the sample rack or the first puncture mechanism to move towards the other one of the sample rack and the first puncture mechanism, so that the first puncture part and the first sealing film are switched from a first position of separation to a second position of puncture of the first sealing film. The device can puncture a plurality of sample tubes simultaneously, avoids the screw caps of the sample tubes, and achieves the purposes of saving time and improving efficiency.
Description
Technical Field
The invention relates to the technical field of nucleic acid sample detection, in particular to a sample transfer device, a sample transfer system and a sample transfer method.
Background
The large-scale rapid screening is the most effective means for preventing and controlling the highly pathogenic infectious diseases, namely 'input cases' and 'asymptomatic infectors'. At present, pathogen nucleic acid samples are collected in a sample tube firstly, a screw cap is screwed on the top opening of the sample tube, the samples are sealed in the sample tube, then the sample tube is transferred into a PCR laboratory, a person is required to manually unscrew the screw cap on the top opening of the sample tube, then the samples in the sample tube are poured into a sample injection hole plate, and then the sample injection hole plate enters a nucleic acid detector for detection.
However, when the samples in the sample tubes are transferred to the sample injection pore plate, the screw caps need to be screwed one by one manually, and if the number of the sample tubes is large, the screw caps are screwed manually only by people to transfer the samples to the sample injection pore plate, so that the working efficiency is low.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the technical defect of low working efficiency of transferring the sample in the sample tube into the orifice plate in the prior art.
Therefore, the invention provides a sample transfer device which comprises a sample rack, a sample rack and a sample tube, wherein the sample rack is provided with at least two mounting channels, and the mounting channels are used for mounting the sample tubes with the bottom parts provided with first sealing membranes capable of being punctured in a one-to-one correspondence manner;
a first lancing mechanism having at least two first lancing portions on the bottom of the sample rack, at least one first lancing portion corresponding to one of the mounting channels;
the first driving part can be connected with one of the sample rack and the first puncture mechanism, and is driven by the driving force to drive the sample rack or the first puncture mechanism to move towards the other one of the sample rack and the first puncture mechanism, so that the first puncture part and the first sealing film are switched from a first position of separation to a second position of puncture of the first sealing film.
The first puncture mechanism further comprises a first mounting seat arranged below the sample rack, all the first puncture parts are mounted on the top of the first mounting seat, and at least one first channel which is arranged on the first mounting seat in a penetrating manner and surrounds the periphery of the first puncture part;
the first drive member may be coupled to one of the first mount and at least two sample tubes.
Further, the first driving part is connected with the upper end of the sample rack, and the bottom of the sample rack is inserted and matched on the first mounting seat;
the first driving part drives the sample tube and the sample rack to move towards the direction close to the first mounting seat, and in the second position state, the bottom of the sample rack is in inserted connection with the first mounting seat.
Furthermore, at least one first locking part is arranged on the sample rack, and second locking parts which correspond to the first locking parts one to one and are matched with the first locking parts in an inserting manner are arranged on the first mounting seat;
one of the first locking part and the second locking part is an elastic clamping groove, the other one of the first locking part and the second locking part is a bulge, and the elastic clamping groove is tightly sleeved on the bulge due to extrusion deformation of the bulge.
Furthermore, the top of the first mounting seat is also provided with accommodating grooves which are correspondingly communicated with the mounting channels one by one, the first puncturing parts are arranged in the accommodating grooves, and the first channels are arranged on the bottoms of the accommodating grooves;
in the second position, the bottom of the sample rack abuts against the top of the first mounting seat, or the bottom of the sample rack is embedded into the accommodating groove, and the top of the first puncture part extends into the mounting channel corresponding to the first puncture part.
Further, in a second position, when the bottom of the sample rack abuts on the top of the first mount; the bottom of the accommodating groove is hermetically provided with a first sealing element in a closed ring shape; the first channel and the first puncture part are distributed in the annular cavity of the first sealing element, and the top of the first sealing element is abutted with the bottom of the sample tube.
Further, at the second position, when the bottom of the sample rack is embedded into the accommodating groove, the bottom of the sample rack is provided with a bracket, and the bracket is provided with third channels corresponding to the mounting channels one to one; the bottom of the sample tube is abutted against the top of the bracket, and the top of the first puncture part extends out of the accommodating groove and penetrates through the third channel to extend into the corresponding mounting channel;
the bottom of the sample rack is matched with the first mounting seat through the bottom of the bracket in an inserting manner.
And furthermore, the device also comprises a sealing gasket arranged on the top of the bracket, wherein the sealing gasket is provided with abdicating holes which are in one-to-one correspondence with the mounting channels and the third channels.
Further, be equipped with on the first mount pad with hold the groove one side surface back to one side with the hollow guide post of holding tank one-to-one intercommunication, hollow guide post is suitable for the one-to-one and inserts in the introduction hole of introduction orifice plate.
Furthermore, the first driving part is provided with second channels which are correspondingly communicated with the mounting channels one by one;
the first driving part is connected with the top of the sample rack in an abutting mode through being sleeved on the outer wall of the sample tube.
Further, the sample rack device also comprises a second driving part arranged above the sample rack; a second sealing piece is hermetically and slidably inserted in the top opening of the sample tube;
in the second position, the second driving part is driven by the driving force to drive the second sealing parts corresponding to the at least two sample tubes to do descending motion relative to the sample tubes.
Further, the sample rack device also comprises a second puncturing mechanism arranged above the sample rack;
the second puncture mechanism comprises a second mounting seat arranged above the sample rack and at least two second puncture parts arranged at the bottom of the second mounting seat, and at least one second puncture part corresponds to a second sealing film which can be punctured and is opened at the top of the sample tube;
in the second position, the second mounting seat is driven by the driving force to drive the second puncturing part to move towards the direction close to the second film sealing part, so that the second puncturing part and the second film sealing part are switched from the third position of separation to the fourth position of puncturing the second film sealing part.
Further, the second puncturing mechanism further comprises
At least one biasing member disposed between the second mount and the top of the sample holder, the second piercing portion being biased to remain in a third position by the biasing force applied by the biasing member.
The invention also provides a sample feeding system, which comprises a sample pretreatment device; and the at least two sample tubes are respectively installed in the installation channels of the sample rack in a one-to-one correspondence manner.
Further, also includes
The sample injection hole plate is provided with sample injection holes which are in one-to-one correspondence with the first sealing films of the sample tubes;
and at the second position, the inner cavities of the sample tubes are communicated with the sample inlet holes in a one-to-one correspondence manner.
The invention also provides a sample transfer method, which comprises the following steps:
installing the sample tubes in the installation channels of the sample rack in a one-to-one correspondence manner;
applying a driving force to the first driving part to enable the first driving part to drive the sample rack and the sample tube connected with the first driving part to integrally move downwards towards the first puncturing part until the first sealing film is punctured by the first puncturing part; or
And applying a driving force to the first puncturing mechanism to enable the first puncturing mechanism to move upwards towards the sample tube until the first sealing film is punctured by the first puncturing part.
The technical scheme of the invention has the following advantages:
1. according to the sample transfer device provided by the invention, the sample tubes are fixed in the installation channel on the sample frame, the driving part can drive the first puncturing mechanism or the sample frame to move towards the other position, so that the first puncturing part and the first sealing membrane are switched from the separated first position to the second position where the first sealing membrane is punctured, the device can puncture a plurality of sample tubes simultaneously, the screwing of the sample tubes is avoided, and the purposes of saving time and improving the efficiency are achieved.
2. The transfer system provided by the invention adopts the sample transfer device, samples in a plurality of sample tubes can be transferred at the same time, and the processed samples can be directly subjected to subsequent operation, so that the processing efficiency is greatly improved.
3. The sample transfer method provided by the invention has the advantages that the driving force is applied to the first driving part or the first puncturing mechanism, so that the first sealing membrane moves towards the other one, the first puncturing part is prompted to puncture the first sealing membrane, and the sample is transferred.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of a first embodiment of the present invention;
FIG. 2 is an exploded view of a first embodiment of the present invention;
FIG. 3 is an exploded view of the sample tube of FIG. 2 with the sample tube removed;
FIG. 4 is a cross-sectional view of a first embodiment of the present invention;
FIG. 5 is another cross-sectional view of the first embodiment of the present invention;
FIG. 6 is a top view of a first piercing structure according to a first embodiment of the present invention;
FIG. 7 is a cross-sectional view taken along line A-A of FIG. 6;
FIG. 8 is a perspective view of a first piercing structure in accordance with a first embodiment of the invention;
FIG. 9 is a schematic structural view of a sample tube according to a first embodiment of the present invention;
FIG. 10 is a schematic view of the structure of a sealing member according to the first embodiment of the present invention;
FIG. 11 is a perspective view of a second embodiment of the present invention;
FIG. 12 is a cross-sectional view of a second embodiment of the present invention;
FIG. 13 is another cross-sectional view of the second embodiment of the present invention;
FIG. 14 is a schematic structural view of a sample tube according to a second embodiment of the present invention;
FIG. 15 is a top view of a first piercing structure according to a second embodiment of the present invention;
FIG. 16 is a cross-sectional view taken along line A-A of FIG. 15;
FIG. 17 is a schematic structural view of a second puncture plate according to a second embodiment of the present invention;
FIG. 18 is a schematic structural view of a bracket according to a second embodiment of the present invention;
FIG. 19 is another structural view of the bracket according to the second embodiment of the present invention;
FIG. 20 is a cross-sectional view of a bracket according to a second embodiment of the invention;
fig. 21 is a schematic structural view of a gasket in a second embodiment of the invention.
Description of reference numerals:
100. a first sample transfer device; 101 a second drive member; 1011. a guide pin; 1012. a top rod; 102. a sample rack; 1021. a sample rack upper plate; 1022. a sample rack lower plate; 1023. a mandril limiting block; 1024. a first locking portion; 103. a first sample tube; 1031. a second seal member; 1032. a first drive member; 1033. a blocking portion; 1034. a first sealing film; 104. a first mounting seat; 1041. a first seal member; 1042. a first channel; 1043. a first piercing section; 1044. accommodating grooves; 1045. a guide post; 1046. a boss; 1047. a second locking portion; 200. a second sample transfer device; 201. a second piercing section; 202. a biasing member; 203. a second sample tube; 2031. a sample tube cover; 2032. a second sealing film; 204. a bracket; 2041. a gasket; 20411. a hole of abdication; 2042. a third channel; 300. a sample injection pore plate; 301. and (4) sampling holes.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The device provided by the invention is described by taking sample transfer in nucleic acid detection pretreatment as an example, collected samples such as pharyngeal swab/nasal swab/blood/saliva/urine of a patient are mixed with virus preservation solution and are filled into a special sample tube, the sample tube is placed into a sample pretreatment device to form a 96 sample tube (not limited to a 96-well plate) array, then the 96 sample tube array is placed into the device provided by the invention to be quickly pretreated, and after the samples are released from the sample tube, the sample tube array is grabbed by a tool such as a manipulator and is thrown into a special recovery barrel.
Example 1
As shown in fig. 14, which is a schematic structural diagram of the second sample tube 203 provided by the present invention, one end of the second sample tube 203 has a sample tube cap 2031, the sample tube cap 2031 is opened and the opening is heat-sealed with a first sealing film 1034, the other end of the second sample tube 203 is heat-sealed with a second sealing film 2032, both the first sealing film 1034 and the second sealing film 2032 are pierceable structures made of aluminum, and may also be other non-toxic and pierceable structures made of sealing films, for example: and the thin film structure is easy to pierce, such as copper, gold, silver and the like.
As shown in fig. 11, 12 and 13, in the second sample transfer device 200 of the present invention, at least two mounting channels are provided in the sample rack 102, and for example, 96 or more than 96 mounting channels may be provided in the sample rack 102 in order to simultaneously puncture 96 second sample tubes 203 in the sample tube array. The second sample tubes 203 are arranged in the mounting channels in a one-to-one correspondence. The first piercing mechanism is disposed below the sample holder 102, and the first piercing mechanism has at least two first piercing portions 1043 capable of piercing the first sealing film 1034, and at least one first piercing portion 1043 corresponds to one installation channel, and it is conceivable that two, three or more first piercing portions 1043 may correspond to one installation channel in order to accelerate the outflow of the sample in the second sample tube 203.
In order to make the first puncture part 1043 of the first puncture mechanism contact with the first sealing film 1034 for puncturing, a first driving part 1032 is provided, the first driving part 1032 is of a flat plate structure, the first driving part 1032 can be connected with the sample holder 102, the first driving part 1032 is located above the sample holder 102, and the sample holder 102 and the second sample tube 203 are moved towards the first puncture part 1043 by applying a driving force from top to bottom; the first driving member 1032 can also be connected to the first puncturing structure, and the first puncturing part 1043 is moved toward the second sample tube 203 by applying a driving force to the first mounting seat 104 of the first puncturing structure from bottom to top, so as to switch the first puncturing part 1043 and the first sealing membrane 1034 from the first position of separation to the second position of puncturing the first sealing membrane. Fig. 12 shows the first puncture portion 1043 and the first seal 1034 in the first position, and fig. 13 shows the first puncture portion 1043 and the first seal 1034 in the second position. The device can process the samples in a plurality of sample tubes simultaneously, and improves the working efficiency.
As shown in fig. 17, in the above embodiment, in order to accelerate the outflow of the sample in the second sample tube 203 by the action of gravity, a second puncturing mechanism may be disposed above the sample rack 102, the second puncturing mechanism includes a second mounting seat (i.e., the first driving member 1032) disposed above the sample rack 102, at least two second puncturing parts 201 are disposed on the bottom of the second mounting seat, the second puncturing parts 201 are puncturing needles, and at least one second puncturing part 201 corresponds to a second pierceable sealing film 2032 of the top opening of the second sample tube 203. A biasing member 202 is disposed between the second mounting seat and the top of the sample holder 102, the biasing member 202 is disposed on the top of the second mounting seat, the biasing member 202 is a member having elasticity, such as a spring, a leaf spring, etc., and the shape of the biasing member 202 may be a cylinder, a table, etc.
As shown in fig. 12, which shows the positional relationship of the second mount to the biasing member 202 when the second mount is not subjected to the driving force or is subjected to the driving force when small; as shown in fig. 13, which shows the position relationship between the second mounting seat and the biasing member 202 when the second mounting seat is subjected to a large or large driving force, the biasing member 202 is deformed by a downward pressure, and when the driving force on the second mounting seat 202 is lost or too small, the elastic force of the biasing member 202 returns the second mounting seat to the position shown in fig. 12, and gas can enter the second sample tube 203 through the punctured hole in the second sealing film 2032, thereby accelerating the flow of the sample out of the second sample tube 203. The position where the second puncturing portion 201 is not in contact with the second sealing film 2032 is referred to as a third position, and the position where the second puncturing portion 201 is in contact with the second sealing film 2032 is referred to as a fourth position.
As shown in fig. 12, 15 and 16, the first puncturing mechanism further includes a first mounting seat 104 disposed below the sample rack 102 and at least one first channel 1042 penetrating the first mounting seat 104 and surrounding the outer periphery of the first puncturing part 1043, all the first puncturing parts 1043 are mounted on the top of the first mounting seat 104, and when the first puncturing part 1043 and the first sealing membrane 1034 are switched from the separated first position to the second position where the first sealing membrane 1034 is punctured, the sample in the second sample tube 203 flows out of the first mounting seat 104 through the first channel 1042. The top of the first mounting seat 104 is further provided with a receiving groove 1044 communicated with the mounting channels in a one-to-one correspondence manner, the first puncturing part 1043 is arranged in the receiving groove 1044, the top of the first puncturing part 1043 is higher than the horizontal plane of the receiving groove 1044, and the first channel 1042 is arranged on the bottom of the receiving groove 1044. In the second position where the first sealing film 1034 is punctured, the bottom of the sample rack 102 is inserted into the receiving groove 1044 through the bracket 204, the top of the first puncturing part 1043 extends into the corresponding mounting channel, specifically, the bottom of the sample rack 102 is provided with the bracket 204, the bracket 204 is provided with the third channels 2042 corresponding to the mounting channels one by one as shown in fig. 18 to 20, and the top of the first puncturing part 1043 extends out of the receiving groove 1044 and into the third channel 2042. The sample holder 102 and the bracket 204 are fixedly attached or abut the top of the bracket 204 through the bottom of the sample holder 102.
The bottom of the sample holder 102 is inserted and matched on the first mounting base 104 through a bracket 204, at least one first locking portion is arranged on the bracket 204, and second locking portions which correspond to the first locking portions one to one and are matched in an insertion mode are arranged on the first mounting base. The first locking portion or the second locking portion is an elastic clamping groove (not shown), and the other is a protrusion (not shown), the elastic clamping groove is tightly sleeved on the protrusion due to extrusion deformation of the protrusion, so that the bracket 204 is prevented from being subjected to external force to cause the first sealing film 1034 of the second sample tube 203 to no longer contact with the first puncturing portion 1043, and thus the outflow of the sample is hindered.
As shown in fig. 18 to 21, the bracket 204 is a holding cavity with an opening, the bottom of the second sample tube 203 abuts against the top of the bracket, the top of the bracket 203 is provided with a gasket 2041, the gasket 2041 is provided with a pair of offset holes 20411 corresponding to the mounting channels and the third channels 2042, and the second sample tube 203 abuts against the top of the bracket 204 through the gasket 2041, so that the sample can be prevented from flowing out of the side wall of the second sample tube 203 when the first sealing film 1034 is punctured.
The first mounting seat 104 is provided with hollow guide pillars (not shown) on a surface opposite to the holding groove 1044 in one-to-one correspondence with the holding groove 1044, and the hollow guide pillars are adapted to be inserted into the sample inlet 301 of the well plate 300 in one-to-one correspondence, so as to flow the sample into the sample inlet 301.
Example 2
The present embodiment provides a sample transfer device 100, and compared with the sample transfer device 200 provided in embodiment 1, the present embodiment adopts a piston rod to push the stable release of the sample, and compared with the gravity-based sample release method adopted in embodiment 1, the sample release efficiency of the present embodiment is higher. In this embodiment, the top of the first puncturing part 1043 of the first puncturing mechanism is lower than the upper surface of the receiving groove 1044, while in embodiment 1, the top of the first puncturing part 1043 of the first puncturing mechanism is higher than the upper surface of the receiving groove 1044; the sample tube provided in this embodiment has a first pierceable sealing film 1034 at one end, and a retractable piston rod is inserted into the other end for sealing, while in embodiment 1, the sample tube has an aluminum film heat-sealed at both ends for piercing; the sample tube of this embodiment is abutted against the sample rack 102 through the blocking part 1033, while in embodiment 1, the sample tube is abutted against the bracket 204 through the bottom of the sample tube, and because the contact position of the piercing end of the sample tube is different in the piercing process, the structural form and the placement position of the sealing structure are also different; due to the difference in the structure of the sample tubes provided in embodiments 1 and 2, in this embodiment the first driving member 1032 is a plate structure having the second channel, whereas in embodiment 1 the first driving member 1032 is a plate structure having the second puncturing part 201. The structure of each component in the first sample transfer device 100 provided in the present embodiment and the cooperation thereof will be specifically described below.
As shown in fig. 1 to 3 and fig. 9, the device provides a first sample tube 103, the first sample tube 103 comprises a first sealing film 1034 having a structure of heat-sealed aluminum film at one end, a blocking portion 1033 at the other end side wall, and a second sealing member 1031 sealingly and slidably inserted into the sample tube, the second sealing member 1031 is a piston rod, and the diameter of at least a portion of the contact portion of the piston rod with the inner wall of the first sample tube 103 is equal to or slightly larger than the diameter of the inner wall of the first sample tube 103.
As shown in fig. 1, the sample rack 102 includes a sample rack upper plate 1021, a sample rack lower plate 1022, and a push rod stopper 1023 connecting the sample rack upper plate 1021 and the sample rack lower plate 1022, at least two installation channels are opened on the sample rack 102, the first sample tubes 103 are completely and consistently abutted against the sample rack 102, and the push rod stopper 1023 can ensure the consistency of the heights between the sample rack upper plate 1021 and the sample rack lower plate 1022. The first sample tubes 103 penetrate the sample rack 102 through the mounting passages one by one and abut against the sample rack upper plate 1021 through the stoppers 1033.
As shown in fig. 6-8, the first piercing mechanism of first sample transfer device 100 and the first piercing mechanism of second sample transfer device 200 each have a first mounting block 104 and a receiving slot 1044, a first piercing portion 1043, and a first channel 1042 disposed on first mounting block 104, except that in this embodiment, the top of first piercing portion 1043 is lower than the upper surface of receiving slot 1044. When the first sealing film 1034 is located at the second position, the end of the first sample tube 103 having the first sealing film 1034 is located in the receiving groove 1044. The first mounting seat 104 is provided with hollow guide pillars 1045, which are in one-to-one correspondence communication with the receiving grooves 1044, on a surface of a side opposite to the receiving grooves 1044, and the hollow guide pillars 1045 are adapted to be inserted into the sample inlet 301 of the well plate 300 in one-to-one correspondence, so as to flow the sample into the sample inlet 301. The first piercing mechanism may have a shape that differs somewhat due to the different configurations of the sample tubes in the first sample transfer device 100 and the second sample transfer device 200.
As shown in fig. 4 and 5, the first drive component 1032 of the first sample transfer device 100 is different from the second sample transfer device 200, the first drive component 1032 of the second sample transfer device 200 is a second mount with the second puncture section 201, and the first drive component 1032 of the first sample transfer device 100 is a flat plate. The first driving part 1032 is provided with second passages communicated with the mounting passages in a one-to-one correspondence, and the second sealing members 1031 pass through the second passages.
As shown in fig. 4, which shows the first puncture 1043 and the first seal 1034 in a first position, and as shown in fig. 5, which shows the first puncture 1043 and the first seal 1034 in a second position. In order to switch the first puncture portion 1043 and the first sealing film 1034 from the separated first position to the second position where the first sealing film 1034 is punctured, a force is applied to the first driving part 1032 by a mechanical arm, an air cylinder, or the like, so as to urge the sample rack 102 and the first sample tube 103 together toward the first puncture portion 1043. In some embodiments, the first driving member 1032 is also coupled to the first mount 104, and is configured to drive the first mount 104 toward the first sample tube 103.
As shown in fig. 2 and 3, the sample rack lower plate 1022 is provided with a first locking portion 1024, and the first mounting base is provided with a second locking portion 1047 which is in one-to-one correspondence with the first locking portion 1024 and can be matched with the first locking portion in an inserting manner. The first locking portion 1024 and the second locking portion 1047 are in a ball-touch structure, that is, one of the first locking portion 1024 and the second locking portion 1047 is an elastic clamping groove, the other is a protrusion, and the elastic clamping groove is tightly sleeved on the protrusion due to extrusion deformation of the protrusion. The lock structure in the second sample transfer device 200 is the same as the first sample transfer device 100, but may take other configurations, both for the same purpose.
As shown in fig. 10, in the receiving groove 1044 provided by the first sample transferring device 100, the bottom of the receiving groove is provided with the first sealing member 1041 in a closed ring, the first passage 1042 and the first puncturing portion 1043 are distributed in the annular cavity of the first sealing member 1041, and the top of the first sealing member 1041 is deformed by being in sealing abutment with the bottom of the first sample tube 103, so as to seal and separate the inner cavity of the first sample tube 103 from the hollow cavity between the outer wall surface of the first sample tube 103 and the inner side wall of the receiving groove, and simultaneously, the sample is prevented from flowing out of the side wall of the first sample tube 103 when the first sealing film 1034 is punctured. In embodiment 1, a gasket 2041 having a plurality of relief holes 20411 is used, and the gasket 2041 is provided on the bracket 204.
As shown in fig. 1 to 3, when the first puncture portion 1043 and the first sealing film 1034 are switched from the first position of separation to the second position where the first sealing film is punctured, the sample flows out by gravity, the flow rate may be relatively slow, the second driving part 101 is provided for increasing the flow rate, the second driving part 101 is located above the sample plate frame 102 and above the first driving part 1032, and in the initial state, the bottom of the second driving part 101 is in contact with the second sealing 1031. In the second position, the second driving member 101 is driven by the driving force applied by the mechanical arm, the cylinder, etc. to move the second seals 1031 corresponding to at least two sample tubes downward relative to the sample tubes, and in order to ensure that the second driving member 101 smoothly pushes the second seals 1031, the second driving member 101 is connected to the sample plate holder 102 by the guide pins 1011, and the second driving member 101 can slide up and down on the guide pins 1011.
In order to avoid the difficulty in pressing down the upper second sealing member 1031 or the slow sample flow rate when the first puncturing mechanism in the first and second sample transfer devices 100 and 200 is pressed against the well plate 300, a boss 1046 is installed on the edge of the first mounting seat 104 opposite to the receiving groove 1044.
In the device provided in embodiment 1, when puncturing is required, a driving force is directly applied to the first driving member 1032 by a mechanical arm, an air cylinder, or the like, so as to urge the sample tube to move toward the first puncturing part 1043, thereby switching the first puncturing part 1043 and the first sealing membrane 1034 from the first position where they are separated to the second position where the first sealing membrane 1034 is punctured, and the second sealing membrane 2032 is also punctured by the second puncturing part 201; in the device provided in embodiment 2, a driving force is first applied directly to the first driving member 1032 by a mechanical arm, an air cylinder, or other mechanism, so as to urge the sample tube to move toward the first puncture portion 1043, and thus the first puncture portion 1043 and the first sealing membrane 1034 are switched from the first position of separation to the second position where the first sealing membrane 1034 is punctured, in this process, since the top rod 1012 is disposed in the top rod limiting block 1023 by an abutting spring, the top rod 1012 will abut against the second driving member 101, so as to prevent the second driving member 101 from moving downward along the guide pin 1011 to push the second sealing member 1031. After the first sealing film 1034 is at the second position where it is pierced, downward pressure is applied to the second driving part 101 by a mechanical arm, an air cylinder, or the like, thereby accelerating the flow-out of the sample in the sample tube.
Example 3
This embodiment provides a transfer system, which includes the first sample transfer device 100 or the second sample transfer device 200, and further includes at least two sample tubes corresponding to the first sample transfer device 100 or the second sample transfer device 200, and the sample tubes are respectively mounted in the mounting channels of the sample rack 102 in a one-to-one correspondence. The pretreatment of the sample tube can be accelerated, and the efficiency is improved.
Meanwhile, the sample injection hole plate 300 is also included, and is provided with sample injection holes 301 which are in one-to-one correspondence with the first sealing films 1034 of the sample tubes; in the second position, the inner cavities of the sample tubes are communicated with the sample inlet holes 301 in a one-to-one correspondence manner. The system works in a pollution-free and closed environment, can quickly preprocess a plurality of sample tubes, can directly carry out subsequent automatic nucleic acid extraction and automatic monitoring after preprocessing, greatly accelerates the processing speed of samples, and promotes the detection capability of large-scale people.
Example 4
The embodiment provides a sample transfer method, which comprises the following steps:
the sample tubes are arranged in the installation channels of the sample rack 102 in a one-to-one correspondence manner;
applying a driving force to the first driving member 1032 to make the first driving member 1032 drive the sample rack and the sample tube connected thereto to move downward integrally toward the first puncturing part 1043 until the first sealing film 1034 is punctured by the first puncturing part 1043; or
A driving force is applied to the first piercing mechanism, so that the first piercing mechanism moves upward toward the sample tube until the first sealing film 1034 is pierced by the first piercing part 1043.
In the above-described embodiment, the first sealing film 1034 is pierced by the first piercing portion 1043 by applying a driving force to the first driving member (1032) or the first piercing mechanism by a mechanism such as a mechanical arm or an air cylinder. The method can be used for processing a plurality of sample tubes simultaneously, and the efficiency is greatly improved.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (12)
1. A sample transfer device, comprising:
the sample holder (102) is provided with at least two mounting channels, and the mounting channels are used for mounting sample tubes with pierceable first sealing films (1034) on the bottoms in a one-to-one correspondence manner;
a first piercing mechanism having at least two first piercing portions (1043) located on a bottom of the sample rack (102), at least one first piercing portion (1043) corresponding to one of the mounting channels;
a first driving member (1032) connectable to one of the sample holder (102) and the first puncturing mechanism, wherein the driving force drives the sample holder (102) or the first puncturing mechanism to move towards the other one of the sample holder (102) and the first puncturing mechanism, so that the first puncturing part (1043) and the first sealing membrane (1034) are switched from a first position of separation to a second position of puncturing of the first sealing membrane (1034).
2. The sample transfer device of claim 1, wherein the first lancing mechanism further comprises a first mount (104) disposed below the sample rack, all of the first lancets (1043) being mounted on top of the first mount (104), and at least one first channel (1042) disposed on the first mount (104) and around the periphery of the first lancets (1043);
the first drive member (1032) is connectable to one of the first mount (104) and at least two sample tubes.
3. The sample transfer device according to claim 2, wherein the first drive member (1032) is connected to an upper end of the sample holder (102), and a bottom of the sample holder (102) is fitted on the first mounting seat (104) in a plug-in manner;
the first driving part (1032) drives the sample tube and the sample rack (102) to move towards the direction close to the first mounting seat (104), and in the second position state, the bottom of the sample rack (102) is in inserted connection with the first mounting seat (104).
4. The sample transfer device according to claim 2 or 3, wherein a receiving groove (1044) in one-to-one communication with the mounting channel is further provided on the top of the first mounting seat (104), the first puncturing part (1043) is provided in the receiving groove (1044), and the first channel is provided on the bottom of the receiving groove (1044);
in the second position, the bottom of the sample rack (102) abuts on the top of the first mounting seat (104), or the bottom of the sample rack (102) is embedded in the accommodating groove (1044), and the top of the first puncturing part (1043) extends into the corresponding mounting channel.
5. The sample transfer device according to claim 4, wherein in the second position, when the bottom of the sample rack (102) abuts on the top of the first mount (104); the bottom of the accommodating groove (1044) is provided with a first sealing element (1041) in a closed ring in a sealing manner; the first channel and the first puncture (1043) are distributed in an annular cavity of the first seal (1041), the top of the first seal (1041) abutting the bottom of the sample tube.
6. The sample transfer device according to claim 4, wherein in the second position, when the bottom of the sample rack (102) is inserted into the receiving groove (1044), the bottom of the sample rack (102) is provided with a bracket (204), and the bracket (204) is provided with a third channel (2042) corresponding to the mounting channel in a one-to-one manner; the bottom of the sample tube abuts against the top of the bracket (204), and the top of the first puncture part (1043) extends out of the accommodating groove (1044) and penetrates through the third channel (2042) to extend into the corresponding mounting channel;
the bottom of the sample rack (102) is inserted and matched on the first mounting seat (104) through the bottom of the bracket (204).
7. The sample transfer device according to claim 4, wherein the first driving member (1032) is provided with second channels which are in one-to-one correspondence communication with the mounting channels;
the first driving part (1032) is arranged in an abutting mode with the top of the sample rack by being sleeved on the outer wall of the sample tube.
8. The sample transfer device according to any of claims 1-7, further comprising a second drive member (101) provided above the sample rack (102); a second sealing member (1031) is sealed on the top opening of the sample tube and can be inserted in a sliding way;
in the second position, the second driving component (101) is driven by the driving force to drive the second sealing members (1031) corresponding to the at least two sample tubes to do descending motion relative to the sample tubes.
9. The sample transfer device according to any of claims 1-7, further comprising a second piercing mechanism (201) provided above the sample holder (102);
the second puncture mechanism (201) comprises a second mounting seat arranged above the sample rack (102), and at least two second puncture parts (201) arranged on the bottom of the second mounting seat, wherein at least one second puncture part (201) corresponds to a second pierceable sealing film (2032) of the top opening of the sample tube;
in the second position, the second mounting seat is driven by the driving force to drive the second puncturing part (201) to move towards the direction close to the second sealing film (2032), so that the second puncturing part (201) and the second sealing film (2032) are switched from the third position of separation to the fourth position of puncturing the second sealing film (2032).
10. The sample transfer device of claim 9, wherein the second lancing mechanism further comprises
At least one biasing member (202) disposed between the second mount and a top of the sample holder (102), the second piercing portion (201) being intended to remain in a third position by a biasing force exerted by the biasing member (202).
11. A transfer system, comprising
A sample pre-treatment apparatus as claimed in any one of claims 1 to 10;
the sample tubes are respectively installed in the installation channels of the sample rack in a one-to-one correspondence manner;
the sample injection hole plate (300) is provided with sample injection holes which correspond to the first sealing films of the sample tubes one by one;
in the second position, the inner cavities of the sample tubes are communicated with the sample inlet holes (301) in a one-to-one correspondence mode.
12. A sample transfer method using the sample transfer device according to any one of claims 1 to 10, comprising the steps of:
the sample tubes are arranged in the installation channels of the sample rack (102) in a one-to-one correspondence manner;
applying a driving force to the first driving part (1032) to enable the first driving part (1032) to drive the sample rack and the sample tube connected with the first driving part to integrally move downwards towards the first puncture part (1043) until the first sealing film (1034) is punctured by the first puncture part (1043); or
And applying a driving force to the first puncturing mechanism to enable the first puncturing mechanism to do ascending motion towards the sample tube until the first sealing film (1034) is punctured by the first puncturing part (1043).
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