WO2023273931A1

WO2023273931A1 - Pre-packaged reagent plate, liquid obtaining device, pre-packaging method, and liquid obtaining method

Info

Publication number: WO2023273931A1
Application number: PCT/CN2022/099762
Authority: WO
Inventors: 易初丽
Original assignee: 武汉医蒂生物科技有限公司
Priority date: 2021-06-30
Filing date: 2022-06-20
Publication date: 2023-01-05
Also published as: CN113477286A

Abstract

A pre-packaged reagent plate, comprising a reagent plate body (1) and seal films. The reagent plate body (1) is provided with at least one through hole (A). The seal films are used for sealing two ports of each through hole (A). An accommodating cavity is formed in the interior of each through hole (A) between the two seal films and is used for accommodating a reaction reagent. The seal films can be pierced to release the reaction reagent.

Description

A prepackaged reagent plate, a liquid-taking device, a pre-packaging method, and a liquid-taking method

This application claims the priority of the Chinese patent application submitted to the China Patent Office on June 30, 2021, with the application number 202110744908.3 and the title of the invention "a prepackaged reagent plate, a liquid collection device, a prepackaged method, and a liquid collection method" , the entire contents of which are incorporated in this application by reference.

technical field

The invention relates to the technical field of test equipment, in particular to a prepackaged reagent plate, a liquid taking device, a prepackaging method and a liquid taking method.

Background technique

When doing experiments, experimenters usually need to use various pipettes to add chemical reagents into test tubes containing samples, so that the chemical reagents and samples undergo a chemical reaction.

There are two commonly used pipetting methods in the prior art. One is manual pipetting with a hand-held pipette, which is inefficient and labor-intensive for the experimenter; the other is pipetting by an automatic pipetting workstation. Liquid, usually includes the following steps: first, add the required reaction reagents into the reagent box in the workstation, secondly, control the pipette tip of the mechanical arm to insert the pipette tip accurately, lift the pipette tip, and then move the The liquid tip moves to the reagent box to absorb the reaction reagent, and then moves to the top of the test tube containing the sample or the holding tank of the standard orifice plate to release the reagent, continuously absorbs and releases the reagent to mix the liquid, and finally, the mechanical arm moves to the trash can for disposal Pipette the tip, the remaining reaction reagents are withdrawn and the kit is washed. The whole process takes a long time. When multiple reagents are required for the experiment, the time required to absorb multiple reagents is longer and the efficiency is lower. In addition, the number of pipetting tips required for pipetting is large and the quality requirements are high. The high cost and the existence of structures such as mechanical arms make the workstation complex in structure, occupying a large space, and low in practicability.

Contents of the invention

In view of this, the present invention provides a prepackaged reagent plate.

The prepackaged reagent plate includes a reagent plate body and a sealing film, the reagent plate body is provided with at least one through hole, and the sealing film is used to seal the two ports of the through hole, inside the through hole, two An accommodating cavity is formed between the sealing films for containing reaction reagents, and the sealing films can be pierced to release the reaction reagents.

Optionally, the sealing film is a plastic aluminum foil film or a plastic film.

Optionally, the shape of the through hole is cylindrical, funnel-shaped or barrel-funnel-shaped.

Optionally, it also includes identification parts arranged on the reagent plate body, and the identification parts correspond to the through holes one by one.

The present invention also provides a liquid-taking device, comprising the aforementioned pre-packaged reagent plate, and a liquid-taking part, and the liquid-taking part is used to puncture the sealing film.

Optionally, the liquid-taking part is a pipette tip.

Optionally, the liquid-taking part is a rod-shaped structure with a tapered lower end.

Optionally, a driving part is also included, and the driving part can fix the liquid-taking part and drive the liquid-taking part to pierce the sealing film.

The present invention also provides a prepackaging method, which is suitable for the aforementioned prepackaging reagent plate, comprising the following steps:

One of the ports is sealed, a predetermined type and a predetermined dose of reaction reagent is injected into the through hole, and the other port is sealed.

The present invention also provides a method for taking liquid, based on the aforementioned prepackaged reagent plate, when the type of the required reaction reagent is one, it includes the following steps:

Place the pre-packaged reagent plate on the top of the sample holding part, align the through hole with the sample holding groove, and puncture the sealing film at both ends of the through hole, so that the reaction reagent falls into the The sample holding tank;

When the kind of required described reaction reagent is multiple, comprise the following steps:

placing the prepackaged reagent plates containing different reaction reagents above the sample holding part in turn, aligning the through hole with the sample holding groove, puncturing the sealing film at both ends of the through hole, so that the reaction reagent falls into the sample holding tank.

Owing to adopted above-mentioned technical scheme, the beneficial effect of the present invention is:

Since in conventional biological experiments, for a certain type and a certain amount of test samples, the type and dosage of the reaction reagents that need to be added are also certain. Therefore, if before the experiment, the preset type and preset quantity The reaction reagents in the prepackaged reagent plate are packaged in the prepackaged reagent plate, then, during the experiment, all the reaction reagents in the prepackaged reagent plate can be directly injected into the sample holding tank to chemically react with the sample.

Based on this, the prepackaged reagent plate of the present invention improves the structure of the reaction reagent containing cavity, specifically, both ends of the containing cavity are set as opening structures, and are sealed by a pierceable sealing film. Before the experiment, according to the experimental requirements, the A preset type and a preset amount of reaction reagents are injected into the holding chamber. During the experiment, the prepackaged reagent plate is directly placed above the sample holding part, and the through hole is aligned with the sample holding groove, and the holes at both ends of the through hole are pierced. Sealing film, the reaction reagent can directly fall into the sample holding tank, and chemically react with the sample, which simplifies the steps of continuously absorbing and releasing the reagent in the prior art. The whole pipetting process is simple and efficient, and the prepackaged reagent plate has a simple structure and low cost Inexpensive, small space occupation, strong practicability.

Description of drawings

Fig. 1 is a schematic structural view of a specific embodiment of a prepackaged reagent plate provided by the present invention;

Fig. 2 is a schematic structural view of the reagent plate body in the prepackaged reagent plate of Fig. 1;

Fig. 3 is a schematic diagram of a row of through holes in the width direction of the prepackaged reagent plate in Fig. 1;

Fig. 4 is an axial sectional view of a single through hole in Fig. 3;

Wherein, the reference numerals in Fig. 1-Fig. 4 are explained as follows:

1-Reagent plate body; A-through hole.

detailed description

In order to enable those skilled in the art to better understand the technical solutions of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

Please refer to FIG. 1 and FIG. 2. FIG. 1 is a schematic structural diagram of a specific embodiment of the prepackaged reagent plate provided by the present invention; FIG. 2 is a schematic structural diagram of the reagent plate body in the prepackaged reagent plate in FIG. 1.

The present invention provides a prepackaged reagent plate, which includes a reagent plate body 1 and a sealing film. The reagent plate body 1 is provided with at least one through hole A, and the sealing film is used to seal the two ports of the through hole A. An accommodating cavity is formed between the two sealing films for containing the reaction reagents, and the sealing films can be pierced to release the reaction reagents.

Because in routine biological experiments, for a certain type and a certain amount of test samples, the type and dosage of the reaction reagents that need to be added are also certain. Add 6 kinds of reagents into the test tube or standard well plate of the sample: 200 μl of cell lysate, 200 μl of nucleic acid adsorption material solution, 350 μl of buffer solution, 600 μl of deproteinized solution, 600 μl of deionized solution and 100 μl of eluent. Therefore, if before the experiment, the reaction reagents of the preset type and the preset quantity are packaged in the pre-packaged reagent plate according to the experimental requirements, then, during the experiment, all the reaction reagents in the pre-packaged reagent plate can be directly injected The sample holding tank is used to chemically react with the sample.

Based on this, the prepackaged reagent plate of the present invention improves the structure of the reaction reagent containing cavity, specifically, both ends of the containing cavity are set as opening structures, and are sealed by a pierceable sealing film. It is necessary to inject a preset type and quantity of reaction reagents into the holding chamber. During the experiment, place the prepackaged reagent plate directly above the sample holding part, and align the through hole A with the sample holding groove, and puncture the through hole A. With the sealing film at both ends of hole A, the reaction reagent can directly fall into the sample holding tank, and chemically react with the sample, simplifying the steps of continuously absorbing and releasing the reagent in the prior art, the whole pipetting process is simple and efficient, and the structure of the prepackaged reagent plate Simple, low cost, small space occupation, wide range of uses and strong practicability.

Among them, the size of the reagent plate body 1, the number of through-holes A, and the distance between two adjacent through-holes A are not strictly limited, and can be compared with experimental consumables that meet international standards, such as 96-well plates, 48-well plates, 24-well plates, etc. The orifice plate, 12-well plate, and 8-well plate are consistent; the volume of the holding chamber is not strictly limited, and it can be adjusted adaptively according to the dose of the reaction reagent required to be accommodated.

The material of the sealing film can be plastic aluminum foil film or plastic film. In practical applications, other materials that are easy to pierce can also be used.

Please refer to Fig. 3-Fig. 4, Fig. 3 is a diagram of a row of through holes in the width direction of the prepackaged reagent plate in Fig. 1; Fig. 4 is an axial cross-sectional view of a single through hole in Fig. 3 .

In the present invention, the shape of the through hole A is not strictly limited, and may specifically be columnar, funnel-shaped, or barrel-funnel-shaped. Wherein, the cylindrical hole includes a cylindrical hole or a prismatic hole and the like. Of course, when the through hole A adopts the barrel funnel shape shown in Figure 2, the upper barrel structure ensures that the through hole A has a larger volume to meet the needs of different types of experiments; During the process, it is possible to prevent the reaction reagent from falling outside the sample holding tank as much as possible, so as to ensure more accurate experimental results, which is a more optimal structural solution.

In addition, as shown in FIG. 1 , the pre-packaged reagent plate in this embodiment also includes an identification component disposed on the reagent plate body 1 , and the identification component corresponds to the through hole A one by one.

Specifically, the through holes A distributed along the length direction are numbered with numbers 1-12, and the through holes A distributed along the width direction are numbered with letters A-H. In this way, each through hole A on the reagent plate body 1 has a unique coordinate , when the types and doses of the reaction reagents contained in each through hole A are different, they can be easily identified and recorded through the corresponding coordinates to ensure that the order and dose of the reaction reagents added during the experiment meet the experimental requirements, thereby ensuring that the experimental the accuracy of the results.

Of course, in practical applications, there may be multiple marking methods, as long as the operator can accurately know the type and dosage of the reaction reagent contained in the through hole A.

The present invention also provides a liquid-taking device, comprising the aforementioned prepackaged reagent plate, and a liquid-taking part, which is used to puncture the sealing film.

The liquid-taking device of the present invention includes the aforementioned prepackaged reagent plate, so it has the same technical effect as that of the aforementioned prepackaged reagent plate, and will not be repeated here.

Wherein, the liquid-taking part can be a pipetting tip, or a simple rod-like structure with a tapered lower end, such as a metal rod, glass rod or plastic rod, as long as the sealing film can be pierced.

In addition, the specific puncturing process can be performed manually or by an automatic device. For example, the liquid-taking device of the present invention also includes a driving part, which can fix the liquid-taking part and drive the liquid-taking part to move to pierce the liquid. The sealing film further improves the efficiency of liquid extraction and reduces the labor intensity of the experimenter.

Specifically, the driving component may be a mechanical arm, or a hydraulic cylinder capable of producing linear displacement.

One of the ports is sealed, a preset type and a preset dose of a reaction reagent is injected into the through hole, and the other port is sealed.

The prepackaging method of the present invention is applicable to the aforementioned prepackaged reagent plates, and therefore has the same technical effect as the aforementioned prepackaged reagent plates, and will not be repeated here.

Among them, when injecting liquid, the required reagent can be injected into the through hole A through an automated filling machine, and the dosage of the reagent type can be adjusted according to the test requirements.

Place the pre-packaged reagent plate on the top of the sample holding part, align the through hole with the sample holding tank, and pierce the sealing film at both ends of the through hole, so that the reaction reagent falls into the sample holding tank and reacts with the sample;

When the kind of required reaction reagent is multiple, comprise the following steps:

Place the pre-packaged reagent plates with different reaction reagents on top of the sample holding parts in turn, align the through holes with the sample holding tanks, and puncture the sealing films at both ends of the through holes, so that the reaction reagents fall into the sample holding tanks in sequence Inside, a chemical reaction occurs sequentially with the sample.

The liquid-taking method of the present invention is applicable to the aforementioned prepackaged reagent plate, and therefore has the same technical effect as the aforementioned prepackaged reagent plate, and will not be repeated here.

In this paper, specific examples are used to illustrate the principle and implementation of the present invention, and the descriptions of the above embodiments are only used to help understand the method and core idea of the present invention. It should be pointed out that for those skilled in the art, without departing from the principles of the present invention, some improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

A prepackaged reagent plate, characterized in that it includes a reagent plate body (1) and a sealing film, the reagent plate body (1) is provided with at least one through hole (A), and the sealing film is used to seal the through hole (A). The two ports of the hole (A), the inside of the through hole (A) and the two sealing membranes form an accommodating chamber for containing the reaction reagent, and the sealing membrane can be pierced to release the Reagents.
The prepackaged reagent plate according to claim 1, wherein the sealing film is a plastic aluminum foil film or a plastic film.
The prepackaged reagent plate according to claim 1, characterized in that, the shape of the through hole (A) is a column, a funnel or a barrel funnel.
The prepackaged reagent plate according to claim 1, characterized in that it further comprises an identification part arranged on the reagent plate body (1), and the identification part is in one-to-one correspondence with the through holes (A).
A liquid-taking device, characterized in that it comprises the prepackaged reagent plate according to any one of claims 1-4, and a liquid-taking part, and the liquid-taking part is used to puncture the sealing film.
The liquid taking device according to claim 5, wherein the liquid taking part is a pipetting tip.
The liquid-taking device according to claim 5, wherein the liquid-taking part is a rod-shaped structure with a tapered lower end.
The liquid-taking device according to claim 5, further comprising a driving component capable of fixing the liquid-taking component and driving the liquid-taking component to pierce the sealing film.
A prepackaging method, applicable to the prepackaging reagent plate described in any one of claims 1-4, characterized in that: comprising the steps of:

sealing one of the ports, injecting a preset type and a preset dose of reaction reagent into the through hole (A), and sealing the other port.
A method for taking liquid, based on the prepackaged reagent plate according to any one of claims 1-4, characterized in that,

When the kind of required described reaction reagent is one kind, comprise the following steps:

Place the pre-packaged reagent plate on the top of the sample holding part, align the through hole (A) with the sample holding groove, and puncture the sealing film at both ends of the through hole, so that the reaction reagent fall into the sample holding tank, and chemically react with the sample;

When the kind of required described reaction reagent is multiple, comprise the following steps:

placing the prepackaged reagent plates containing different reaction reagents above the sample holding part in turn, aligning the through hole with the sample holding groove, puncturing the sealing film at both ends of the through hole, The reaction reagents are sequentially dropped into the sample holding tank, and chemically react with the samples sequentially.