CN112154911A - Technology for high-yield hybrid rice with reeds - Google Patents

Technology for high-yield hybrid rice with reeds Download PDF

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CN112154911A
CN112154911A CN202011111603.0A CN202011111603A CN112154911A CN 112154911 A CN112154911 A CN 112154911A CN 202011111603 A CN202011111603 A CN 202011111603A CN 112154911 A CN112154911 A CN 112154911A
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rice
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钱存堂
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Liaoning Qiandao Agriculture Co ltd
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Liaoning Qiandao Agriculture Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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Abstract

The invention discloses: a technology for high-yield hybrid rice with reed includes such steps as hybridizing wild rice with Hejiang 19 to obtain the first generation rice, hybridizing with Hejiang 19 to obtain the second generation rice, hybridizing with wild barnyard grass as male parent to obtain the third generation rice, hybridizing with Hejiang 19 to obtain the fourth generation rice, and hybridizing with Longjing 8 male parent to obtain the fifth generation rice.

Description

Technology for high-yield hybrid rice with reeds
Technical Field
The invention is applied to the field of agriculture, and particularly relates to a technology for high-yield hybrid rice of reeds.
Background
Hybrid rice (hybrid rice) refers to the first generation hybrid species with heterosis produced by hybridizing two rice varieties which have certain differences in heredity and can complement each other at the same time, and is used for production. Heterosis is a common phenomenon in the biological world, and the utilization of heterosis to improve the yield and quality of crops is one of the main achievements of modern agricultural science. The rice hybridization which is successful for the first time in the world is completed by Henry Beachell in Indonesia in 1963 and then awarded to the world grain prize in 1996, the rice has obvious heterosis phenomena which are mainly shown in the aspects of vigorous growth, developed root system, more panicle and large grain, strong stress resistance and the like, so that the utilization of the heterosis of the rice to greatly improve the yield of the rice is always a desire of a breeder to dread. However, rice is easy to cause some corresponding diseases when being planted at ordinary times, and thus the phenomenon of yield reduction is easy to occur.
Disclosure of Invention
The invention aims to provide a technology for high-yield hybrid rice of reed, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a technology for high-yield hybrid rice with reed includes such steps as hybridizing wild rice with Hejiang 19 to obtain the first generation rice, hybridizing with Hejiang 19 to obtain the second generation rice, hybridizing with wild barnyard grass as male parent to obtain the third generation rice, hybridizing with Hejiang 19 to obtain the fourth generation rice, and hybridizing with Longjing 8 as male parent to obtain the fifth generation rice.
Preferably, distant hybridization is carried out by taking second-generation rice as a female parent and taking wild reed as a male parent to obtain third-generation rice.
Preferably, the third generation rice is hybridized with the early indica No. 3 to obtain the fourth generation rice.
Preferably, the third generation rice is hybridized with the Shannong fragrant glutinous rice to obtain the fourth generation rice.
Preferably, genetically stable material is obtained by selection after 2 to 5 generations of continuous cultivation of four generation rice.
Preferably, the genetic stable material is obtained by selecting after 4 to 8 generations of continuous cultivation of five generations of rice.
Compared with the prior art, the hybrid rice bred by the invention has the advantages that:
1. the average yield of the rice is improved by more than 25 percent.
2. Improving the disease resistance of rice (rice blast, bacterial wilt, damping off and banded sclerotial blight).
3. Improve the lodging resistance of rice (root system hereditary reed, developed and stout vascular bundle, deep ground grabbing of root hair and large area)
4. Improve the insect pest resistance of rice (stem genetic reed, net structure, pest difficult to gnaw)
5. Can be used for saline-alkali soil treatment (PH10 high saline-alkali soil, can be planted for land treatment)
Detailed Description
In order that the above objects, features and advantages of the present invention may be more clearly understood, there is described in detail further below with reference to specific embodiments. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced otherwise than as specifically described and thus the scope of the present invention is not limited by the specific embodiments disclosed below.
Example 1
Referring to claims 1, 5 and 6, the present invention provides a technical solution: a technology for high-yield hybrid rice with reed includes such steps as hybridizing wild rice with Hejiang 19 to obtain the first generation rice, hybridizing with Hejiang 19 to obtain the second generation rice, hybridizing with wild barnyard grass as male parent to obtain the third generation rice, hybridizing with Hejiang 19 to obtain the fourth generation rice, and hybridizing with Longjing 8 male parent to obtain the fifth generation rice.
Specifically, genetically stable materials are obtained by selection after 2 to 5 generations of continuous cultivation of four-generation rice.
Specifically, after 4-8 generations of rice are continuously cultivated, genetic stable materials are obtained by selection.
The working principle is as follows: a first generation; selection of wild rice stock: the wild rice variety named as Meyeriana is a conventional parent germplasm resource for breeding new rice varieties in China. Hejiang No. 19 selection: hejiang 19 is bred by rice of agriculture academy of Heilongjiang province, inherits all advantages of Hejiang 12, Jingyin 59 and Jingyin 58, the culture area of the variety is stabilized to more than 100 ten thousand mu after 1985, the variety is planted in the crown of the rice variety of Heilongjiang province for a long time, the wild rice with wart grains is hybridized with Hejiang 19, the rice is transplanted and cultured, the mature period is 125 days, and the activity temperature is accumulated by 2300 degrees.
A second generation; the primary rice bred by hybridization is respectively collected and classified into the following four standards according to spike type, grain type, stem stout degree and overall height: wild-synthesized initial spike-A, wild-synthesized initial spike-A1, wild-synthesized initial spike-B1, wild-synthesized initial grain-A1, wild-synthesized initial grain-B1, wild-synthesized initial stem-A1, wild-synthesized initial stem-B1, wild-synthesized initial height-A1, wild-synthesized initial height-B and wild-synthesized initial height-B1. Respectively backcrossed with Hejiang No. 19
And a third generation: screening the obtained backcross progeny, eliminating experimental varieties with narrower leaves, weak stalks, loose plants and infected node plague, preferably selecting strong progeny with the average plant height of 90 cm, the ear length of 15 cm and no awns as follows: wild-bred generation A resistant, wild-bred generation B resistant, wild-bred generation straw A, wild-bred generation straw B, wild-bred generation 26A and wild-bred generation 26B are used as female parent, wild barnyard grass and reed are used as male parent, and distant hybridization is respectively carried out.
Fourth generation: the obtained distant filial generation is continuously hybridized with Hejiang No. 19 material and continuously compared in variety
Fourth generation: the genetic stable material is obtained by selection of 2 to 5 generations, and has the characteristics of rice blast resistance, banded sclerotial blight resistance, bacterial wilt resistance, strong fiber tissue structure, insect pest bite resistance and other disease and insect pest resistance, low temperature cold damage resistance, full spike shape, long grains in the grain shape, thousand grain weight of 26 g and a ripening period of 130 days.
And a fifth generation: hybridizing the stable material as female parent and Longjing No. 8 as male parent
And a fifth generation: the reed rice is bred by continuous 6 generations of selection, which successfully has the super-strong lodging-resistant capability of the reed, the stout degree of the stem is 0.5-1 times of that of the conventional rice, the root system depth reaches 50 cm, and the ground-grabbing capability is extremely strong. The fiber structure of the vascular bundle is a net shape which replicates the characteristics of the reed, the width and the length of the leaf are more than 40 cm, and the height of the leaf is 110-120 cm. The reed rice has salt resistance and alkali resistance, and can be used as a saline-alkali soil treatment material for soil ecological improvement.
Actual test data
Figure BDA0002728773860000041
In summary, the yield is relatively uniform and suitable for normal culture.
Example two
Please refer to claims 1, 2, 3, 5, and 6, the present invention provides a technical solution: a technology for high-yield hybrid rice with reed includes such steps as hybridizing wild rice with Hejiang 19 to obtain the first generation rice, hybridizing with Hejiang 19 to obtain the second generation rice, hybridizing with wild barnyard grass as female parent to obtain the third generation rice, hybridizing with Hejiang 19 to obtain the fourth generation rice, and hybridizing with Longjing 8 male parent to obtain the fifth generation rice.
Specifically, distant hybridization is carried out by taking second-generation rice as female parent and wild reed as male parent to obtain third-generation rice.
Specifically, the third generation rice is hybridized with the Shannong fragrant glutinous rice to obtain the fourth generation rice.
Specifically, genetically stable materials are obtained by selection after 2 to 5 generations of continuous cultivation of four-generation rice.
Specifically, after 4-8 generations of rice are continuously cultivated, genetic stable materials are obtained by selection.
The working principle is as follows: a first generation; selection of wild rice stock: the wild rice variety named as Meyeriana is a conventional parent germplasm resource for breeding new rice varieties in China. Hejiang No. 19 selection: hejiang 19 is bred by rice of agriculture academy of Heilongjiang province, inherits all advantages of Hejiang 12, Jingyin 59 and Jingyin 58, the culture area of the variety is stabilized to more than 100 ten thousand mu after 1985, the variety is planted in the crown of the rice variety of Heilongjiang province for a long time, the wild rice with wart grains is hybridized with Hejiang 19, the rice is transplanted and cultured, the mature period is 125 days, and the activity temperature is accumulated by 2300 degrees.
A second generation; the primary rice bred by hybridization is respectively collected and classified into the following four standards according to spike type, grain type, stem stout degree and overall height: wild-synthesized initial spike-A, wild-synthesized initial spike-A1, wild-synthesized initial spike-B1, wild-synthesized initial grain-A1, wild-synthesized initial grain-B1, wild-synthesized initial stem-A1, wild-synthesized initial stem-B1, wild-synthesized initial height-A1, wild-synthesized initial height-B and wild-synthesized initial height-B1. Respectively backcrossed with Hejiang No. 19
And a third generation: screening the obtained backcross progeny, eliminating experimental varieties with narrower leaves, weak stalks, scattered plants and infected node plagues, and classifying strong progeny without awns into the following categories: carrying out distant hybridization by taking wild first generation anti-A, wild first generation anti-B, wild first generation straw A, wild first generation straw B, wild first generation straw 26A and wild first generation straw 26B as female parents and taking wild barnyard grass and reed as male parents respectively.
Fourth generation: the obtained distant filial generation is continuously hybridized with Hejiang No. 19 material and continuously compared in variety
Fourth generation: the genetic stable material is obtained by selection of 2 to 5 generations, has the characteristics of disease and pest resistance such as rice blast resistance, banded sclerotial blight resistance and bacterial wilt resistance, strong fiber tissue structure, pest and bite resistance and the like, is resistant to low temperature cold damage, has full spike shape and long grain size.
And a fifth generation: hybridizing the stable material as female parent and Longjing No. 8 as male parent
And a fifth generation: the reed rice is bred by continuous 6 generations of selection, which successfully has the super-strong lodging-resistant capability of the reed, the stout degree of the stem is 0.5-1 times of that of the conventional rice, the root system depth reaches 50 cm, and the ground-grabbing capability is extremely strong. The fiber structure of the vascular bundle is a net shape which replicates the characteristics of the reed, the width and the length of the leaf are more than 40 cm, and the height of the leaf is 110-120 cm. The reed rice has salt resistance and alkali resistance, and can be used as a saline-alkali soil treatment material for soil ecological improvement.
Actual test data
Figure BDA0002728773860000051
In conclusion, the effect of increasing the yield by increasing the ratio of the ear length is better than that of the common effect.
EXAMPLE III
Please refer to claims 1, 2, 3, and 5, the present invention provides a technical solution: a technology for high-yield hybrid rice with reed includes such steps as hybridizing wild rice with Hejiang 19 to obtain the first generation rice, hybridizing the second generation rice with Hejiang 19 to obtain the second generation rice, hybridizing the first generation rice with wild barnyard grass as male parent to obtain the third generation rice, hybridizing the third generation rice with Hejiang 19 to obtain the fourth generation rice, and hybridizing the fourth generation rice with Longjing 8 as female parent to obtain the fifth generation rice.
Specifically, distant hybridization is carried out by taking second-generation rice as female parent and wild reed as male parent to obtain third-generation rice.
Specifically, the third generation rice is hybridized with the Shannong fragrant glutinous rice to obtain the fourth generation rice.
Specifically, genetically stable materials are obtained by selection after 2 to 5 generations of continuous cultivation of four-generation rice.
Specifically, after 4-8 generations of rice are continuously cultivated, genetic stable materials are obtained by selection.
The working principle is as follows: a first generation; selection of wild rice stock: the wild rice variety named as Meyeriana is a conventional parent germplasm resource for breeding new rice varieties in China. Hejiang No. 19 selection: hejiang 19 is bred by rice of agriculture academy of Heilongjiang province, inherits all advantages of Hejiang 12, Jingyin 59 and Jingyin 58, the culture area of the variety is stabilized to more than 100 ten thousand mu after 1985, the variety is planted in the crown of the rice variety of Heilongjiang province for a long time, the wild rice with wart grains is hybridized with Hejiang 19, the rice is transplanted and cultured, the mature period is 125 days, and the activity temperature is accumulated by 2300 degrees.
A second generation; the primary rice bred by hybridization is respectively collected and classified into the following four standards according to spike type, grain type, stem stout degree and overall height: wild-synthesized initial spike-A, wild-synthesized initial spike-A1, wild-synthesized initial spike-B1, wild-synthesized initial grain-A1, wild-synthesized initial grain-B1, wild-synthesized initial stem-A1, wild-synthesized initial stem-B1, wild-synthesized initial height-A1, wild-synthesized initial height-B and wild-synthesized initial height-B1. Respectively backcrossed with Hejiang No. 19
And a third generation: screening the obtained backcross progeny, eliminating experimental varieties with narrower leaves, weak stalks, scattered plants and infected node plagues, and classifying strong progeny without awns into the following categories: carrying out distant hybridization by taking wild first generation anti-A, wild first generation anti-B, wild first generation straw A, wild first generation straw B, wild first generation straw 26A and wild first generation straw 26B as female parents and taking wild barnyard grass and reed as male parents respectively.
Fourth generation: the obtained distant filial generation is continuously hybridized with Hejiang No. 19 material and continuously compared in variety
Fourth generation: the genetic stable material is obtained by selection of 2 to 5 generations, has the characteristics of disease and pest resistance such as rice blast resistance, banded sclerotial blight resistance and bacterial wilt resistance, strong fiber tissue structure, pest and bite resistance and the like, is resistant to low temperature cold damage, has full spike shape and long grain size.
And a fifth generation: hybridizing the stable material as female parent and Longjing No. 8 as male parent
Actual test data
Figure BDA0002728773860000071
In conclusion, the effect of increasing the yield by increasing the ratio of the ear length is better than that of the common effect, but the lodging resistance is poorer, and the root system is shorter.
Example four
Please refer to claims 1, 2, 3, and 6, the present invention provides a technical solution: a technology for high-yield hybrid rice with reed includes such steps as hybridizing wild rice with Hejiang 19 to obtain the first generation rice, hybridizing the second generation rice with Hejiang 19 to obtain the second generation rice, hybridizing the first generation rice with wild barnyard grass as male parent to obtain the third generation rice, hybridizing the third generation rice with Hejiang 19 to obtain the fourth generation rice, and hybridizing the fourth generation rice with Longjing 8 as female parent to obtain the fifth generation rice.
Specifically, distant hybridization is carried out by taking second-generation rice as female parent and wild reed as male parent to obtain third-generation rice.
Specifically, the third generation rice is hybridized with the Shannong fragrant glutinous rice to obtain the fourth generation rice.
Specifically, genetically stable materials are obtained by selection after 2 to 5 generations of continuous cultivation of four-generation rice.
Specifically, after 4-8 generations of rice are continuously cultivated, genetic stable materials are obtained by selection.
The working principle is as follows: a first generation; selection of wild rice stock: the wild rice variety named as Meyeriana is a conventional parent germplasm resource for breeding new rice varieties in China. Hejiang No. 19 selection: hejiang 19 is bred by rice of agriculture academy of Heilongjiang province, inherits all advantages of Hejiang 12, Jingyin 59 and Jingyin 58, the culture area of the variety is stabilized to more than 100 ten thousand mu after 1985, the variety is planted in the crown of the rice variety of Heilongjiang province for a long time, the wild rice with wart grains is hybridized with Hejiang 19, the rice is transplanted and cultured, the mature period is 125 days, and the activity temperature is accumulated by 2300 degrees.
A second generation; the primary rice bred by hybridization is respectively collected and classified into the following four standards according to spike type, grain type, stem stout degree and overall height: wild-synthesized initial spike-A, wild-synthesized initial spike-A1, wild-synthesized initial spike-B1, wild-synthesized initial grain-A1, wild-synthesized initial grain-B1, wild-synthesized initial stem-A1, wild-synthesized initial stem-B1, wild-synthesized initial height-A1, wild-synthesized initial height-B and wild-synthesized initial height-B1. Respectively backcrossed with Hejiang No. 19
And a third generation: screening the obtained backcross progeny, eliminating experimental varieties with narrower leaves, weak stalks, scattered plants and infected node plagues, and classifying strong progeny without awns into the following categories: carrying out distant hybridization by taking wild first generation anti-A, wild first generation anti-B, wild first generation straw A, wild first generation straw B, wild first generation straw 26A and wild first generation straw 26B as female parents and taking wild barnyard grass and reed as male parents respectively.
Fourth generation: the obtained distant filial generation is continuously hybridized with Hejiang No. 19 material and continuously compared in variety
And a fifth generation: hybridizing the stable material as female parent and Longjing No. 8 as male parent
And a fifth generation: the reed rice is bred by continuous 6 generations of selection, which successfully has the super-strong lodging-resistant capability of the reed, the stout degree of the stem is 0.5-1 times of that of the conventional rice, the root system depth reaches 50 cm, and the ground-grabbing capability is extremely strong. The fiber structure of the vascular bundle is a net shape which replicates the characteristics of the reed, the width and the length of the leaf are more than 40 cm, and the height of the leaf is 110-120 cm. The reed rice has salt resistance and alkali resistance, and can be used as a saline-alkali soil treatment material for soil ecological improvement.
Actual test data
Figure BDA0002728773860000081
In conclusion, the results of increasing the yield by increasing the ratio of the ear length are better than the results of the conventional method, but the disease resistance is poorer than that of the rice in other test fields, and the stability is insufficient.
In the description of the present specification, the terms "connect", "mount", "fix", and the like are intended to be broadly construed, for example, "connect" may be a fixed connection, a detachable connection, or an integral connection; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the description herein, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (6)

1. A technology for high-yield hybrid rice with reed includes such steps as hybridizing wild rice with Hejiang 19 to obtain the first generation rice, hybridizing with Hejiang 19 to obtain the second generation rice, hybridizing with wild barnyard grass as male parent to obtain the third generation rice, hybridizing with Hejiang 19 to obtain the fourth generation rice, and hybridizing with Longjing 8 as male parent to obtain the fifth generation rice.
2. The technology of hybrid rice with high yield of reeds as claimed in claim 1, wherein; carrying out distant hybridization by taking the second generation rice as a female parent and taking wild reed as a male parent to obtain the third generation rice.
3. The technology of hybrid rice with high yield of reeds as claimed in claim 1, wherein; the third generation rice is hybridized with the first generation glutinous rice to obtain the fourth generation rice.
4. The technology of hybrid rice with high yield of reeds as claimed in claim 1, wherein; the third generation rice is hybridized with the early indica No. 3 to obtain the fourth generation rice.
5. The technology of hybrid rice with high yield of reeds as claimed in claim 1, wherein; after 2-5 generations of rice are continuously cultivated, genetic stable materials are obtained by selection.
6. The technology of hybrid rice with high yield of reeds as claimed in claim 1, wherein; after the five generations of rice are continuously cultivated from 4 to 8 generations, genetic stable materials are obtained by selection.
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008025185A2 (en) * 2006-08-17 2008-03-06 Fudong Liao A rice breeding method for fixing heterosis
CN101142894A (en) * 2007-09-26 2008-03-19 云南省农业科学院生物技术与种质资源研究所 Wild-rice distant hybridization high-efficient cultivating superior progeny method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008025185A2 (en) * 2006-08-17 2008-03-06 Fudong Liao A rice breeding method for fixing heterosis
CN101142894A (en) * 2007-09-26 2008-03-19 云南省农业科学院生物技术与种质资源研究所 Wild-rice distant hybridization high-efficient cultivating superior progeny method

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
仇秀丽等: "野生稻在种间杂交育种中利用的研究进展", 《云南农业大学学报》 *
何强等: "水稻远缘杂交及杂种优势利用现状", 《中国农业科技导报》 *
傅雪琳等: "利用种间杂交途径向栽培稻转移非AA组野生稻有利基因的研究进展", 《中国水稻科学》 *
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