CN112136599A - Automatic screening and optimizing device for optical imaging assisted edible mushroom breeding - Google Patents
Automatic screening and optimizing device for optical imaging assisted edible mushroom breeding Download PDFInfo
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- CN112136599A CN112136599A CN202011089863.2A CN202011089863A CN112136599A CN 112136599 A CN112136599 A CN 112136599A CN 202011089863 A CN202011089863 A CN 202011089863A CN 112136599 A CN112136599 A CN 112136599A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
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Abstract
An optical imaging auxiliary automatic screening optimization device for edible fungus breeding belongs to the technical field of bioengineering breeding, and comprises an object stage, a main guide rail, an auxiliary guide rail and an imaging instrument fixer; when the device is used, firstly, edible strains are placed in a grid area of a table top of an objective table, after parameter setting and initialization are carried out on an upper computer, a main guide rail controls the transverse movement of an imaging instrument, a secondary guide rail controls the longitudinal movement of the imaging instrument, the imaging instrument acquires image information of the edible strains, the upper computer analyzes images to extract phenotypes, and a servo motor and the imaging instrument work in a set period. The invention reduces the manual supervision and intervention, and greatly improves the information acquisition efficiency and accuracy during the breeding and screening of the edible fungi.
Description
Technical Field
The invention belongs to the technical field of bioengineering breeding, and particularly relates to an automatic screening and optimizing device for optical imaging assisted edible fungus breeding.
Background
The edible fungus breeding refers to the mating among different species or different strains in the species, and generally comprises the stages of hybridization pairing, pipe transfer propagation, preliminary screening, secondary screening, cultivation test, demonstration popularization and the like. In the primary screening and secondary screening stages, measurement and calculation are required: 1. the growth rate of hyphae; 2. phenotypic characteristics of fruiting mass (bag); 3. (ii) a phenotypic characteristic of a fruit body; 4. yield of fruit body; 5. suitable for growth temperature, humidity, etc. The measurement calculation methods commonly used at present include: the calipers measure the scale information of hypha, fungus block (bag) and sporocarp, and an optical imaging instrument is used manually to obtain the phenotype information of the shape, the water content and the like of the fungus. The optical imaging enhances the information richness of phenotype extraction, reduces the destructiveness to thalli, and is an effective assistant tool for breeding.
The objects of primary screening and secondary screening of edible fungus breeding are various hybrid varieties, a large amount of manual thallus information acquisition is needed, the efficiency of frequent manual determination is low for real-time detection, and entry registration errors are easily caused, so that the breeding progress and accuracy are influenced. Combine optical imaging means, design an automatic sieving mechanism of domestic fungus breeding, the phenotype information of regularly monitoring the thallus, replace artifical survey, avoid human error or error, can improve domestic fungus breeding efficiency and success rate.
Disclosure of Invention
In the edible fungus breeding and screening stage, in order to realize the automatic monitoring and screening function of phenotypes of various strains, the invention provides an optical imaging auxiliary edible fungus breeding automatic screening and optimizing device which mainly comprises an object stage and a sliding guide rail, wherein the object stage is divided into equal grids for placing various strains, the sliding guide rail is divided into a main guide rail and an auxiliary guide rail, the main guide rail is used for carrying imaging instruments such as a visible light camera, a multispectral camera and a fluorescence camera, the imaging instruments can transversely move on the main guide rail, and the main guide rail can longitudinally move on the auxiliary guide rail so as to ensure that the imaging instruments can cover each grid of the object stage. The specific technical scheme is as follows:
an optical imaging auxiliary automatic screening and optimizing device for edible fungus breeding comprises an object stage 1, a main guide rail 2, an auxiliary guide rail 3 and an imaging instrument fixer 4;
the object stage 1 consists of a supporting angle 5 and a table top 6, the table top 6 is uniformly divided into a plurality of grid areas, each grid area is used for culturing an edible strain, and the grid areas are placed in a circular culture dish identification area 7 in the middle of the grid;
the main body structure of the main guide rail 2 consists of a cross beam 8 and a support frame 9 which are formed by connecting triangular fixing supports 10, the upper part and the lower part of the inside of the cross beam 8 are respectively provided with a rubber guide rail bar 11, and two concave wheels 12 with servo motors are occluded between the two rubber guide rail bars 11;
the main body structure of the auxiliary guide rail 3 is a cross beam 8, which is similar to the main guide rail, a rubber guide rail bar 11 is respectively arranged at the upper part and the lower part in the cross beam 8 of the auxiliary guide rail, and two concave wheels 12 with servo motors are occluded between the two rubber guide rail bars 11;
the main guide rail 2 and the auxiliary guide rail 3 are connected together through a triangular connector 13, the stability of the structure is ensured, and the axis of the concave wheel 12 is connected with the bottom edge vertex angle of the triangular bracket 13;
the imaging instrument fixer 4 is connected with the axis of the concave wheel 12 through a connector 14, the lower end of the imaging instrument fixer 4 comprises three imaging instrument fixing positions 15 for fixing imaging instruments 16 (including a visible light camera, a multispectral camera and a fluorescence camera), and the fixing positions 15 can transversely rotate to adjust the angle of the imaging instruments;
a servo motor on the main guide rail controls the concave wheel 12 to rotate, so that the imaging instrument 16 transversely moves above the table board 6;
a servo motor on the auxiliary guide rail controls the concave wheel 12 to rotate, so that the main guide rail moves longitudinally, and the imaging instrument 16 moves longitudinally above the table top 6;
under the motion control of the main guide rail and the auxiliary guide rail, the imaging instrument 16 regularly shoots and images the edible strains of each grid on the table board 6 one by one, and transmits the images back to the upper computer for processing and analysis to obtain related phenotype information;
the upper computer records the coding state of the servo motor, determines a grid area corresponding to the edible strain detected by the current camera, and simultaneously the visible light camera collects image information and feeds the image information back to the servo motor control system by identifying the circular culture dish identification area to adjust the position so as to obtain the optimal observation point position.
The use method of the optical imaging assisted edible fungus breeding automatic screening optimization device comprises the following steps:
step 1:
placing each cultured edible strain culture dish in a circular culture dish identification area 7 in a grid area of a table board 6, and setting growth conditions such as temperature, humidity and the like;
step 2:
resetting an upper computer for controlling the servo motor, the imaging instrument and image analysis, setting the working period of the imaging system, executing initialization by the upper computer, and restoring the imaging instrument holder 4 to the initial position;
and step 3: in a working period, the upper computer controls servo motors on the main guide rail 2 and the auxiliary guide rail 3, the imaging instrument fixer 4 is moved, an edible strain on the table top is traversed to shoot an image, wherein the multispectral camera works all day long, the visible light camera works in the daytime, the fluorescent camera works at night, the image is analyzed by the upper computer, phenotype information such as water content, component content and growth vigor of the edible strain is analyzed and obtained from the multispectral image, the phenotype information such as the form, scale and the like of the edible strain is analyzed and obtained from the visible light image and the fluorescent image, and various phenotype information is registered;
and 4, step 4:
after the growth period of the edible fungi is finished, the computer comprehensively analyzes the collected and registered phenotype information, and retains the edible strains of good varieties by combining the existing discrimination standard.
Compared with the existing edible fungus breeding and screening operation means, the optical imaging assisted edible fungus breeding automatic screening and optimizing device has the beneficial effects that:
the main guide rail and the auxiliary guide rail realize the full coverage of the imaging instrument on the table grid area, the accurate control of the servo motor provides a foundation for the automatic operation of image acquisition, and the efficiency of phenotype information acquisition is greatly improved;
secondly, the positioning precision of the camera is further improved by combining image analysis and circular target detection of the upper computer;
thirdly, the ID of each grid area can be conveniently obtained by utilizing an encoder of the servo motor, so that the automatic arrangement of the data entries is realized;
the three different imaging instruments complement each other, more phenotype information is extracted, and data verification is carried out, so that the accuracy of information extraction is improved;
and fifthly, the object stage has longitudinal expandability so as to realize the edible fungus breeding experiment with larger sample scale.
Drawings
FIG. 1 is a schematic diagram of the overall structure of an optical imaging-assisted edible fungus breeding automatic screening optimization device of the present invention;
FIG. 2 is a schematic view of an objective table of the optical imaging assisted edible fungus breeding automatic screening optimization device of the present invention;
FIG. 3 is a schematic diagram of a main guide rail of an optical imaging-assisted automatic screening and optimizing device for edible fungus breeding according to the present invention;
FIG. 4 is a schematic diagram of a secondary guide rail of the optical imaging assisted edible fungus breeding automatic screening optimization device of the present invention;
FIG. 5 is a schematic view of an imaging instrument holder of the optical imaging assisted edible fungus breeding automatic screening optimization device of the present invention.
Wherein: 1-objective table, 2-main guide rail, 3-auxiliary guide rail, 4-imaging instrument fixer, 5-objective table support angle, 6-objective table top, 7-circular culture dish identification area, 8-beam, 9-main guide rail support frame, 10-triangle fixing support, 11-rubber guide rail bar, 12-concave wheel with servo motor, 13-triangle connector, 14-imaging instrument connector, 15-imaging instrument fixing point and 16-imaging instrument.
Detailed Description
The present invention will be further described with reference to specific examples, but the present invention is not limited to these examples.
Example 1
An optical imaging auxiliary automatic screening and optimizing device for edible fungus breeding comprises an object stage 1, a main guide rail 2, an auxiliary guide rail 3 and an imaging instrument fixer 4;
the object stage 1 consists of a supporting angle 5 and a table top 6, the table top 6 is uniformly divided into 100 multiplied by 100 grid areas, each grid area is used for culturing an edible strain, and the circular culture dish identification area 7 is placed in the center of the grid;
the main body structure of the main guide rail 2 consists of a cross beam 8 and a support frame 9 which are formed by connecting triangular fixing supports 10, the upper part and the lower part of the inside of the cross beam 8 are respectively provided with a rubber guide rail bar 11, and two concave wheels 12 with servo motors are occluded between the two rubber guide rail bars 11;
the main body structure of the auxiliary guide rail 3 is a cross beam 8, which is similar to the main guide rail, a rubber guide rail bar 11 is respectively arranged at the upper part and the lower part in the cross beam 8 of the auxiliary guide rail, and two concave wheels 12 with servo motors are occluded between the two rubber guide rail bars 11;
the main guide rail 2 and the auxiliary guide rail 3 are connected together through a triangular connector 13, the stability of the structure is ensured, and the axis of the concave wheel 12 is welded with the bottom edge vertex angle of the triangular bracket 13;
the imaging instrument holder 4 is connected with the axis of the concave wheel 12 through a connector 14, and the lower end of the imaging instrument holder 4 comprises three imaging instrument fixing points 15 for fixing imaging instruments 16 (including a visible light camera, a multispectral camera and a fluorescence camera);
a servo motor on the main guide rail controls the concave wheel 12 to rotate, so that an imaging instrument 16 can transversely monitor the table top 6;
a servo motor on the auxiliary guide rail controls the concave wheel 12 to rotate, so that the main guide rail moves longitudinally, and the imaging instrument 16 monitors the table top 6 longitudinally;
under the motion control of the main guide rail and the auxiliary guide rail, the imaging instrument 16 regularly shoots and images the edible strains of each grid on the table board 6 one by one, and transmits the images back to the upper computer for processing and analysis to obtain related phenotype information;
the upper computer records the coding state of the servo motor, determines a grid area corresponding to the edible strain detected by the current camera, and simultaneously the visible light camera collects image information and feeds the image information back to the servo motor control system by identifying the circular culture dish identification area to adjust the position to obtain the optimal observation point position;
a use method of an optical imaging assisted edible fungus breeding automatic screening optimization device comprises the following steps:
step 1:
placing each cultured edible strain culture dish in a circular culture dish identification area 7 in a grid area of a table board 6, and setting a group of growth environments: the temperature was 28 ℃ and the humidity was 80%.
Step 2:
resetting an upper computer for controlling the servo motor, the imaging instrument and image analysis, setting the working period of the imaging system to be 2 hours, executing initialization by the upper computer, and restoring the imaging instrument fixer 4 to an initial position;
and step 3: in a working period, the upper computer controls servo motors on the main guide rail 2 and the auxiliary guide rail 3, the imaging instrument fixer 4 is moved, the edible strain on the table top is traversed to shoot images, wherein the multispectral camera works all day long, the visible light camera works in the daytime (8: 00-18: 00), the fluorescent camera works in the nighttime (18: 00-8: 00), the images are analyzed by the upper computer, the phenotypic information of the edible strain, such as the water content, the component content, the growth vigor and the like, is analyzed and obtained from the multispectral images, the phenotypic information of the edible strain, such as the form, the scale and the like, is analyzed and obtained from the visible light images and the fluorescent images, and the phenotypic information of each item is registered;
and 4, step 4:
after the growth period of the edible fungi is finished, the computer comprehensively analyzes the collected and registered phenotype information, and retains the edible strains of good varieties by combining the existing discrimination standard.
The utility model provides an automatic screening optimization device of supplementary domestic fungus breeding of optical imaging, during the device use, at first place the edible bacterial strain in the grid region of objective table mesa, carry out parameter setting and initialization back to the host computer, the lateral shifting of main guide rail control imaging instrument, the longitudinal movement of vice guide rail control imaging instrument, the image information of edible bacterial strain is gathered to the imaging instrument, carry out the analysis by the host computer to the image and draw the phenotype, servo motor and imaging instrument work in the settlement cycle. The invention reduces the manual supervision and intervention and greatly improves the information acquisition efficiency and accuracy.
Claims (9)
1. An optical imaging assisted automatic screening and optimizing device for edible fungus breeding is characterized by comprising an object stage 1, a main guide rail 2, an auxiliary guide rail 3 and an imaging instrument fixer 4; the objective table 1 consists of a supporting angle 5 and a table top 6, and the edible bacterial strains are placed in a circular culture dish identification area 7 in the middle of the grid;
the main structure of the main guide rail 2 consists of a cross beam 8 and a support frame 9 which are connected by a triangular fixed support 10; a rubber guide rail bar 11 is respectively arranged at the upper part and the lower part in the beam 8, and a concave wheel 12 is positioned between the two rubber guide rail bars 11;
the main body structure of the auxiliary guide rail 3 is a cross beam 8, which is similar to the main guide rail; the main guide rail 2 and the auxiliary guide rail 3 are connected together through a triangular connector 13;
the imaging instrument holder 4 is connected with the axis of the concave wheel 12 through a connector 14, and the lower end of the imaging instrument holder 4 comprises three imaging instrument fixing points 15 for fixing imaging instruments 16 (including a visible light camera, a multispectral camera and a fluorescence camera).
2. The automatic screening and optimizing device for edible fungi breeding assisted by optical imaging as claimed in claim 1, wherein the table top 1 is equally divided into a plurality of grids, and the center of each grid is marked by a geometric figure with a regular shape for placing edible fungi.
3. The automated screening and optimizing apparatus for edible fungi breeding assisted by optical imaging as claimed in claim 1, wherein the main guide rail 2 uses two concave wheels to ensure the stability of the imaging instrument holder.
4. The automatic screening and optimizing device for edible fungus breeding assisted by optical imaging as claimed in claim 1, wherein the concave wheels 12 of the auxiliary guide rail 3 are connected with the support frame 9 of the main guide rail 2 by triangular connectors 13, so as to ensure the stability of the main rail 2.
5. The automatic screening and optimizing device for edible fungus breeding assisted by optical imaging as claimed in claim 1, wherein a servo motor on the main guide rail 2 controls the concave wheel 12 to rotate, so that the imaging instrument 16 moves transversely above the table-board 6.
6. The automated edible fungus breeding screening and optimizing device assisted by optical imaging according to claim 1, wherein a servomotor on the secondary guide rail 3 controls the concave wheel 12 to rotate, so that the primary guide rail moves longitudinally, and the imaging instrument 16 moves longitudinally above the table top 6.
7. The automated screening and optimizing apparatus for edible fungi breeding assisted by optical imaging as claimed in claim 1, wherein the fixing points 15 of the imaging instrument holder can be laterally adjusted in angle to ensure alignment with the target.
8. The automatic screening and optimizing device for edible fungus breeding assisted by optical imaging as claimed in claim 1, wherein under the control of the motion of the primary guide rail and the secondary guide rail, the imaging instrument 16 periodically takes pictures and images of edible strains of each grid on the table-board 6 one by one, feeds back the pictures to a control system of a private server motor through the coding state of a servo motor and the identification of the circular culture dish identification area 7, and adjusts the position to obtain the best observation point position.
9. The use method of the optical imaging assisted edible fungus breeding automatic screening optimization device, as recited in claim 1, comprises the following steps:
step 1:
placing each cultured edible strain culture dish in a circular culture dish identification area 7 in a grid area of a table board 6, and setting growth conditions such as temperature, humidity and the like;
step 2:
resetting an upper computer for controlling the servo motor, the imaging instrument and image analysis, setting the working period of the imaging system, executing initialization by the upper computer, and restoring the imaging instrument holder 4 to the initial position;
and step 3: in a working period, the upper computer controls servo motors on the main rail 2 and the auxiliary rail 3, the imaging instrument fixer 4 is moved, an edible strain on the table top is traversed to shoot an image, wherein the multispectral camera works all day long, the visible light camera works in the daytime, the fluorescent camera works at night, the image is analyzed by the upper computer, phenotype information such as water content, component content and growth vigor of the edible strain is analyzed and obtained from the multispectral image, the phenotype information such as the form, scale and the like of the edible strain is analyzed and obtained from the visible light image and the fluorescent image, and various phenotype information is registered;
and 4, step 4:
after the growth period of the edible fungi is finished, the computer comprehensively analyzes the collected and registered phenotype information, and retains the edible strains of good varieties by combining the existing discrimination standard.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113966693A (en) * | 2021-10-25 | 2022-01-25 | 福建万辰生物科技股份有限公司 | Method for rapidly screening needle mushroom in breeding stage |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113966693A (en) * | 2021-10-25 | 2022-01-25 | 福建万辰生物科技股份有限公司 | Method for rapidly screening needle mushroom in breeding stage |
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