CN112113822A - Biological sample dyeing device, push piece dyeing machine and biological sample dyeing method - Google Patents
Biological sample dyeing device, push piece dyeing machine and biological sample dyeing method Download PDFInfo
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- CN112113822A CN112113822A CN201910542258.7A CN201910542258A CN112113822A CN 112113822 A CN112113822 A CN 112113822A CN 201910542258 A CN201910542258 A CN 201910542258A CN 112113822 A CN112113822 A CN 112113822A
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- 239000012472 biological sample Substances 0.000 title claims abstract description 65
- 238000004043 dyeing Methods 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000009971 piece dyeing Methods 0.000 title claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 382
- 230000007246 mechanism Effects 0.000 claims abstract description 123
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 39
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 238000010186 staining Methods 0.000 claims description 58
- 230000002572 peristaltic effect Effects 0.000 claims description 17
- 239000011259 mixed solution Substances 0.000 claims description 13
- 230000001105 regulatory effect Effects 0.000 claims description 13
- 239000000523 sample Substances 0.000 claims description 9
- 238000010521 absorption reaction Methods 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 description 12
- 239000008280 blood Substances 0.000 description 12
- 239000007853 buffer solution Substances 0.000 description 12
- 238000010586 diagram Methods 0.000 description 11
- 239000000243 solution Substances 0.000 description 10
- 239000000872 buffer Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000004891 communication Methods 0.000 description 3
- 230000009977 dual effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
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- 230000003583 cytomorphological effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The application discloses a biological sample dyeing device, a push piece dyeing machine and a biological sample dyeing method, wherein the biological sample dyeing device comprises a reagent bearing mechanism for storing mixed liquid; the turbulent flow structure is provided with a flow channel for conveying liquid, is used for turbulent flow and uniformly mixing the liquid in the flow channel, and conveys the mixed liquid to the reagent bearing mechanism; the first liquid inlet pipe is connected with the turbulent flow structure and used for conveying first liquid; the second liquid inlet pipe is connected with the first liquid inlet pipe in parallel on the turbulent flow structure and is used for conveying a second liquid; the driving mechanism is arranged between the turbulent flow structure and the first liquid inlet pipe, is arranged between the turbulent flow structure and the second liquid inlet pipe, and is used for providing liquid inlet power for the first liquid and the second liquid; and the control mechanism is electrically connected with the driving mechanism to control the first liquid inlet pipe or the second liquid inlet pipe to be communicated with the turbulent flow structure.
Description
Technical Field
The application relates to the technical field of biological sample dyeing, in particular to a biological sample dyeing device, a push piece dyeing machine and a biological sample dyeing method.
Background
In the examination of biological samples such as blood samples, it is generally required to uniformly coat the blood sample on a slide glass, place the blood sample on the slide glass in a staining container with a staining solution for staining, and then send the blood sample to an analysis instrument such as a microscope for cytomorphological analysis. The dyeing solution is usually prepared by adopting a certain proportion of initial dyeing solution and buffer solution. In the existing push piece dyeing all-in-one machine, the initial dye liquor and the buffer liquor are respectively input into a dye liquor container, and the dye liquor is obtained by uniformly mixing in the dye liquor container. However, in this way, the feeding flow rates of the primary dye solution and the buffer solution are relatively high, so that the requirements on related air path driving parts are high, and the raw material cost of the push piece and dyeing integrated machine is increased.
Disclosure of Invention
The invention provides a biological sample dyeing device, a push piece dyeing machine and a biological sample dyeing method.
According to a first aspect of the embodiments of the present application, there is provided a biological sample staining apparatus, including a reagent carrying mechanism for storing a mixed solution; the turbulent flow structure is provided with a flow channel for conveying liquid, is used for turbulent flow and uniformly mixing the liquid in the flow channel, and conveys the mixed liquid to the reagent bearing mechanism; the first liquid inlet pipe is connected to the turbulent flow structure and used for conveying first liquid; the second liquid inlet pipe is connected to the turbulence structure in parallel with the first liquid inlet pipe and is used for conveying a second liquid; the driving mechanism is arranged between the turbulence structure and the first liquid inlet pipe, arranged between the turbulence structure and the second liquid inlet pipe and used for providing liquid inlet power for the first liquid and the second liquid; and the control mechanism is electrically connected with the driving mechanism to control the first liquid inlet pipe or the second liquid inlet pipe to be communicated with the turbulent flow structure.
According to a second aspect of embodiments of the present application, there is provided a push slide dyeing machine comprising a slide loading device for loading slides; a sample application device for loading a biological sample onto the slide; the slide pushing device is used for flattening the biological sample on the slide to make a smear; the biological sample staining device as described above, for staining the smear.
According to a third aspect of the embodiments of the present application, there is provided a biological sample dyeing method for the above-mentioned push-piece dyeing machine, including: absorbing a first liquid with a first preset absorption amount and a second liquid with a second preset absorption amount into the turbulent flow structure; disturbing the first liquid and the second liquid by the disturbing structure to obtain a mixed liquid; and staining the smear by using the mixed solution.
The technical scheme provided by the embodiment of the application can have the following beneficial effects:
the application designs a biological sample dyeing device, a push piece dyeing machine and a biological sample dyeing method, wherein when a control mechanism is matched with a driving mechanism to enable a first liquid inlet pipe to be communicated with a turbulent flow structure, first liquid is sucked into the turbulent flow structure from the first liquid inlet pipe; when control mechanism and actuating mechanism cooperation made second feed liquor pipe and vortex structure intercommunication, the vortex structure was inhaled from second feed liquor pipe to the second liquid, first liquid and second liquid realized the misce bene in vortex structure department, the mixed liquid that obtains after the misce bene flows into reagent bearing mechanism, not only can adopt the initial dyeing liquor and the buffer solution of arbitrary ratio to make the mixed liquid of misce bene and will mix liquid input reagent bearing mechanism, moreover, the steam circuit actuating mechanism that the liquid feeding velocity of flow required height compares, actuating mechanism's raw and other materials cost has been reduced.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
Fig. 1 is a schematic view of an angle structure of a blade-pushing dyeing machine according to an embodiment of the present application;
FIG. 2 is a schematic view of another angle of the blade-pushing dyeing machine according to an embodiment of the present application;
FIG. 3 is a schematic structural diagram of a biological sample staining apparatus according to an embodiment of the present application;
FIG. 4 is a schematic block diagram of the biological specimen staining apparatus of FIG. 3;
FIG. 5 is a schematic structural diagram of a biological sample staining apparatus according to an embodiment of the present application;
FIG. 6 is a schematic block diagram of the biological specimen staining apparatus of FIG. 5;
FIG. 7 is a schematic structural diagram of a biological sample staining apparatus according to an embodiment of the present application;
FIG. 8 is a schematic block diagram of the biological specimen staining apparatus of FIG. 7;
FIG. 9 is a schematic structural diagram of a biological sample staining apparatus according to an embodiment of the present application;
FIG. 10 is a schematic block diagram of the biological specimen staining apparatus of FIG. 9;
FIG. 11 is a schematic structural view of a biological sample staining apparatus according to an embodiment of the present application;
FIG. 12 is a schematic block diagram of the biological specimen staining apparatus of FIG. 11;
FIG. 13 is a schematic structural view of a biological sample staining apparatus according to an embodiment of the present application;
FIG. 14 is a schematic block diagram of the biological specimen staining apparatus of FIG. 13;
FIG. 15 is a pre-staining diagram of a staining apparatus for biological samples according to an embodiment of the present application;
FIG. 16 is a pre-staining view of a staining apparatus for biological samples according to another embodiment of the present application;
fig. 17 is a schematic flow chart of a method for staining a biological sample according to an embodiment of the present application.
Description of reference numerals:
10. a sampling device; 20. a slide loading device; 30. a sample adding device; 40. a blade pushing device;
50. a biological sample staining device; 51. a reagent carrying mechanism; 52. a turbulent flow structure; 521. a spiral tube; 522. a first connecting branch pipe; 523. a second connecting branch pipe; 524. a first connector sub-tube; 525. a helical sub-tube; 526. a second connector sub-tube; 53. a first liquid inlet pipe; 54. a second liquid inlet pipe; 55. a drive mechanism; 551. a first pump body; 552. a second pump body; 553. a first syringe; 554. a second syringe; 555. a third syringe;
56. a control mechanism; 561. a controller; 562. a control valve; 5621. a first valve port; 5622. a second valve port; 5623. a third valve port; 5631. a first regulating valve; 5632. a second regulating valve; 564. a first valve; 5641. a first port; 5642. a second port; 5643. a third port; 565. a second valve; 5651. a first opening; 5652. a second opening; 5653. a third opening; 566. an automatic valve; 5661. a first inlet; 5662. a second inlet; 5663. an outlet; 567. a control valve switch; 5671. a first interface; 5672. a second interface; 5673. a third interface;
571. a proportioning input module; 572. a dyeing pre-display module; 581. a first liquid supply container; 582. a second liquid supply container; 60. smearing; 70. a first liquid; 80. a second liquid.
Detailed Description
The present application will be described in further detail below with reference to the accompanying drawings by way of specific embodiments. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).
The present embodiment provides a push-piece staining machine for staining of biological samples. The biological sample includes, but is not limited to, microorganisms, blood, body fluids, and bone marrow fluid. In order to express the concept of the present application more clearly, the following description will be made by taking blood as a biological sample and a push-piece dyeing machine for dyeing the biological sample as an example.
Referring to fig. 1 and 2, in some embodiments, a slide-dyeing machine may be used for smear preparation of biological samples such as blood. The slide dyeing machine comprises a sampling device 10 for sucking a blood sample, a slide loading device 20 for moving a slide to a working line, a loading device 30 for loading the blood sample to a slide 60, a slide pushing device 40 for smearing the blood sample on the slide to obtain a smear 60, a drying device (not shown) for drying a blood film on the smear 60, and a biological sample dyeing device 50 for dyeing the smear 60.
The glass slide may be made of glass, or any other suitable material may be used.
Referring to fig. 3 to 14, in one embodiment, the biological sample staining apparatus 50 includes a reagent loading mechanism 51, a turbulent flow structure 52, a first liquid inlet pipe 53, a second liquid inlet pipe 54, a driving mechanism 55, and a control mechanism 56.
The reagent holding mechanism 51 is used for storing the mixed solution. In some embodiments, the reagent carrying mechanism 51 may be a staining bath, and the smear 60 obtained after being pushed by the pushing piece device 40 is stained in the reagent carrying mechanism 51. In other embodiments, the reagent carrying mechanism 51 may also be a pre-mixing tank, and the mixed solution flowing out from the turbulent flow structure 52 may flow into the reagent carrying mechanism 51 to be further mixed to obtain a dyeing solution, so as to ensure that the dyeing solution is sufficiently and uniformly mixed. In the technical scheme of drop dyeing, the reagent carrying mechanism can also be a drop dyeing needle or a drop dyeing tube and is used for dropping the dyeing liquid onto the smear 60 for dyeing. In order to express the concept of the present application more clearly, the reagent carrying mechanism is described as a staining bath as an example.
The flow perturbation structure 52 has a flow channel (not shown) for transporting liquid. The turbulent flow structure 52 is a structure capable of changing a liquid flowing through a part or all of the flow channels from a laminar flow to a turbulent flow. Different liquids flowing into the turbulent flow structure 52 can be quickly disturbed and uniformly mixed after forming turbulent flow, so that different liquids in the turbulent flow uniformly-mixing flow channel can be realized, and mixed liquid can be obtained. The turbulent flow mechanism 52 sends the resulting mixed solution to the reagent carrying mechanism 51 to stain the smear 60.
Specifically, the spoiler structure 52 may be a tubular structure having a bent portion, a tubular structure having a spiral portion, or the like. When the baffle structure 52 is a tubular structure having a helical portion, the helical portion may be formed by using a pipe having any suitable inner diameter and any suitable length, for example, a hose having an inner diameter of 3.2mm and a length of 2000mm may be used to form a helical portion having a helical diameter of 40 mm.
The first and second inlet pipes 53 and 54 are used to transport the first and second liquids 70 and 80, respectively. The second inlet pipe 54 is connected to the baffle structure 52 in parallel with the first inlet pipe 53. The driving mechanism 55 is disposed between the turbulent flow structure 52 and the first liquid inlet pipe 53, and disposed between the turbulent flow structure 52 and the second liquid inlet pipe 54, for providing liquid inlet power for the first liquid 70 and the second liquid 80.
The control mechanism 56 is electrically connected to the driving mechanism 55 to control the first liquid inlet pipe 53 or the second liquid inlet pipe 54 to communicate with the turbulent structure 52. Specifically, referring to fig. 4, 6, 8, 10, 12 and 14, the control mechanism 56 includes a controller 561, and the controller 561 is electrically connected to the driving mechanism 55, so as to control the driving mechanism 55 to start and stop.
It will be appreciated that the controller 561 may first control the operation of the driving mechanism 55 to control the first liquid inlet 53 to communicate with the flow disturbing structure 52 for delivering the first liquid 70 to the flow disturbing structure 52 or control the second liquid inlet 54 to communicate with the flow disturbing structure 52 for delivering the second liquid 80 to the flow disturbing structure 52. In some embodiments, the controller 561 may alternatively control the first liquid inlet pipe 53 to communicate with the flow disturbing structure 52 and the second liquid inlet pipe 54 to communicate with the flow disturbing structure 52 until the total injection amount of the mixed liquid flowing into the reagent carrying mechanism 51 through the flow disturbing structure 52 reaches a predetermined injection threshold.
Referring to fig. 4, 6, 8, 10, 12 and 14, in some embodiments, the biological sample staining apparatus 50 further includes a ratio input module 571 and a staining pre-display module 572, so as to facilitate a user to set mixed solutions with different ratios. The ratio input module 571 is electrically connected to the controller 561 of the control mechanism 56, and is configured to input a target ratio of the first liquid 70 to the second liquid 80. The staining pre-display module 572 is electrically connected to the controller 561 of the control mechanism 56, and is configured to display a pre-staining graph of the biological sample at a target ratio.
Specifically, the target ratio of the first liquid 70 and the second liquid 80 can be set to any suitable ratio according to actual needs. The user may directly input the target ratio of the first liquid 70 to the second liquid 80, or may set the target ratio by pulling a scroll bar or the like. The dyeing pre-display module 572 can visually display a pre-dyeing image, so that a user can know the pre-dyeing effect in the target ratio in advance, the user can know the pre-dyeing effect in advance without trying to dye, and the proportion of the first liquid 70 to the second liquid 80 can be designed directly as required. The user can be a doctor or a scientific research staff.
For example, when the target ratio of the first liquid 70 to the second liquid 80 is 1:18, the pre-dyeing pattern displayed by the dyeing pre-display module 572 is as shown in fig. 15. When the target ratio of the first liquid 70 to the second liquid 80 is 1:9, the pre-dyeing pattern displayed by the dyeing pre-display module 572 is as shown in fig. 16.
Referring to fig. 3, 5, 7, 9, 11, and 13, in some embodiments, the biological specimen staining apparatus 50 further includes a first liquid supply container 581 and a second liquid supply container 582. The first liquid supply container 581 is communicated with the first liquid inlet pipe 53 for storing the first liquid 70. The second liquid supply vessel 582 is in communication with the second liquid inlet conduit 54 for storing the second liquid 80.
It should be noted that, the first liquid 70 is different from the second liquid 80, the first liquid 70 may be an initial dye solution, the second liquid 80 is a buffer solution, the initial dye solution refers to a high-concentration dye solution, and the mixed solution refers to a liquid obtained by diluting and mixing the initial dye solution with the buffer solution. Of course, the first liquid 70 and the second liquid 80 may be any other suitable liquids different from each other. For example, the first liquid 70 is a buffer solution, the second liquid is an initial dyeing liquid, and for the sake of more clear expression of the concept of the present application, the following explanation will take the first liquid 70 as the initial dyeing liquid and the second liquid 80 as the buffer solution as an example.
In the biological sample staining apparatus 50 of the above embodiment, when the control mechanism 56 cooperates with the driving mechanism 55 to communicate the first liquid inlet pipe 53 with the turbulent flow structure 52, the first liquid 70 is sucked into the turbulent flow structure 52 from the first liquid inlet pipe 53; when control mechanism 56 and actuating mechanism 55 cooperation made second feed liquor pipe 54 and vortex structure 52 communicate, second liquid 80 inhales vortex structure 52 from second feed liquor pipe 54, first liquid 70 and second liquid 80 realize the misce bene in vortex structure 52 department, the mixed liquid that obtains after the misce bene flows into reagent bearing mechanism 51, not only can adopt the initial dyeing liquid and the buffer solution of arbitrary ratio to make the mixed liquid of misce bene and input mixed liquid into reagent bearing mechanism 51, moreover, the steam circuit actuating mechanism that the liquid feeding velocity of flow required height compares, actuating mechanism's raw and other materials cost has been reduced.
It will be appreciated that in some embodiments, the first liquid 70 and the second liquid 80 may be alternately sucked into the flow perturbation structure 52 until the amount of the first liquid added reaches the first predetermined suction amount and the amount of the second liquid added reaches the second predetermined suction amount, which may enable a more uniform mixing of the first liquid 70 and the second liquid 80 at the flow perturbation structure 52. The first preset suction volume and the second preset suction volume can be set according to actual needs.
Of course, in other embodiments, the first liquid 70 and the second liquid 80 may be simultaneously or time-divisionally sucked into the turbulent flow structure 52, as long as the adding amount of the first liquid reaches the first preset sucking amount, and the adding amount of the second liquid reaches the second preset sucking amount, which is not limited in this application. In order to more clearly express the concept of the present application, the first liquid 70 and the second liquid 80 are alternately sucked into the flow disturbing structure 52 as an example.
Referring to fig. 3 to 10, in some embodiments, the turbulent flow structure 52 includes a spiral pipe 521, a first connecting branch pipe 522 and a second connecting branch pipe 523.
Referring to fig. 3, 5, 7 and 9, one end of the spiral pipe 521 is connected to the reagent loading mechanism 51. In some embodiments, the spiral pipe 521 extends along the conveying direction of the liquid to form a spiral channel to which the first liquid 70 and the second liquid 80 flow, and are turned inside the spiral channel to be mixed uniformly. The first connecting branch pipe 522 has a liquid inlet end and a liquid outlet end, the liquid inlet end of the first connecting branch pipe 522 is connected to the first liquid inlet pipe 53, and the liquid outlet end of the first connecting branch pipe 522 is connected to the other end of the spiral pipe 521.
Referring to fig. 3, 5, 7 and 9, the second connecting branch pipe 523 has an inlet end and an outlet end, the inlet end of the second connecting branch pipe 523 is connected to the second liquid inlet pipe 54, and the outlet end of the second connecting branch pipe 523 is connected to the other end of the spiral pipe 521, so that the first liquid inlet pipe 53 and the second liquid inlet pipe 54 are connected to the spiral pipe 521 in parallel. The driving mechanism 55 is disposed between the first connecting branch pipe 522 and the first liquid inlet pipe 53, and the driving mechanism 55 is disposed between the second connecting branch pipe 523 and the second liquid inlet pipe 54.
Referring to fig. 3 and 5, in some embodiments, the driving mechanism 55 is a dual channel peristaltic pump including a pump body defining a first channel and a second channel. The first connecting branch pipe 522 is connected to the first liquid inlet pipe 53 through the first passage. The second branch connecting pipe 523 is connected to the second liquid inlet pipe 54 through the second passage. In some embodiments, the conduit portions within the first and second channels are both flexible tubes. The pump body is operable to alternately compress and release the conduit portion within the first channel to provide inlet fluid power to the first fluid. Of course, the pump body may alternatively squeeze and release the pipe sections in the second channel to provide the inlet motive force for the second liquid.
Referring to fig. 3 and 4, in some embodiments, the control mechanism 56 further includes a control valve 562 electrically connected to the controller 561. The control valve 562 includes a first port 5621, a second port 5622, and a third port 5623, the first port 5621 is connected to the outlet end of the first branch connecting pipe 522, the second port 5622 is connected to the outlet end of the second branch connecting pipe 523, the third port 5623 is connected to the solenoid 521, and the third port 5623 is controllably communicated with the first port 5621 or the second port 5622. The controller 561 controls the communication time of the third valve port 5623 between the first valve port 5621 and the second valve port 5622, thereby controlling the charging ratio of the first liquid 70 and the second liquid 80.
Referring to fig. 3 and 4, in this embodiment, the controller 561 controls the actuation of the drive mechanism 55 (in this embodiment, a dual channel peristaltic pump). The controller 561 controls the operation of the control valve 562 such that the third port 5623 of the control valve 562 communicates with the first port 5621 of the control valve 562, thereby communicating the solenoid 521 with the first connecting branch 522. At this time, under the driving of the two-channel peristaltic pump, the first liquid 70, i.e. the initial dye liquor, enters the spiral pipe 521 through the first liquid inlet pipe 53 and the first connecting branch pipe 522. After the third port 5623 of the control valve 562 is communicated with the first port 5621 of the control valve 562 for a first preset time, the controller 561 controls the control valve 562 to operate, so that the third port 5623 of the control valve 562 is communicated with the second port 5622 of the control valve 562 for a second preset time, and the solenoid 521 is communicated with the second connecting branch 523. At this time, under the driving of the two-channel peristaltic pump, the second liquid 80, i.e. the buffer solution, enters the spiral pipe 521 through the second liquid inlet pipe 54 and the second connecting branch pipe 523, and the first liquid 70 and the second liquid 80 are uniformly mixed at the spiral pipe 521 to form a mixed solution and flow into the reagent carrying mechanism 51. The above steps are alternately executed, so that the first liquid 70 and the second liquid 80 are alternately sucked into the spiral pipe 521, until the adding amount of the first liquid 70 reaches the first preset sucking amount, and the adding amount of the second liquid 80 reaches the second preset sucking amount, the controller 561 controls the dual-channel peristaltic pump to be closed.
It can be understood that the first preset time and the second preset time can be set according to actual requirements. For example, the first preset time is 0.2S, and the second preset time is 3.6S.
Referring to fig. 5 and 6, in some embodiments, the control mechanism 56 further includes a first regulating valve 5631 and a second regulating valve 5632 electrically connected to the controller 561, and the controller 561 controls the respective opening times of the first regulating valve 5631 and the second regulating valve 5632, so as to control the charging ratio of the first liquid and the second liquid. A first regulating valve 5631 is provided on the first connecting branch 522. The second regulating valve 5632 is provided to the second connecting branch 523.
Referring to fig. 5 and 6, in this embodiment, the controller 561 controls the actuation of the drive mechanism 55 (in this embodiment, a dual channel peristaltic pump). The controller 561 controls the first regulating valve 5631 to be opened such that the first inlet pipe 53 communicates with the solenoid 521 through the first connecting branch pipe 522. At this time, under the driving of the two-channel peristaltic pump, the first liquid 70, i.e. the initial dye liquor, enters the spiral pipe 521 through the first liquid inlet pipe 53 and the first connecting branch pipe 522. After the first adjusting valve 5631 is opened for a first preset time, the controller 561 controls the first adjusting valve 5631 to be closed and the second adjusting valve 5632 to be opened, so that the second liquid inlet pipe 54 is communicated with the spiral pipe 521 through the second connecting branch pipe 523 for a second preset time. It will be appreciated that the coil 521 may be connected to the first and second connector legs 522, respectively, via a tee. At this time, under the driving of the two-channel peristaltic pump, the second liquid 80, i.e. the buffer solution, enters the spiral pipe 521 through the second liquid inlet pipe 54 and the second connecting branch pipe 523, and the first liquid 70 and the second liquid 80 are uniformly mixed at the spiral pipe 521 to form a mixed solution and flow into the reagent carrying mechanism 51. The above steps are alternately executed, so that the first liquid 70 and the second liquid 80 are alternately sucked into the spiral pipe 521, until the adding amount of the first liquid 70 reaches the first preset sucking amount, and the adding amount of the second liquid 80 reaches the second preset sucking amount, the controller 561 controls the dual-channel peristaltic pump to be closed.
Referring to fig. 7 and 8, in some embodiments, the driving mechanism 55 includes a first pump 551 and a second pump 552, and the controller 561 controls different working times of the first pump 551 and the second pump 552, so as to control the charging ratio of the first liquid and the second liquid. The first pump 551 is disposed between the first connecting branch pipe 522 and the first inlet pipe 53. The second pump body 552 is disposed between the second connecting branch pipe 523 and the second liquid inlet pipe 54.
Referring to fig. 7 and 8, in this embodiment, the controller 561 controls the first pump 551 to open, and the first liquid 70, i.e. the initial dyeing liquid, enters the spiral pipe 521 through the first liquid inlet pipe 53 and the first connecting branch pipe 522. After the first pump 551 is opened for a first preset time, the controller 561 controls the first pump 551 to close and the second pump 552 to open, at this time, the second liquid 80, i.e. the buffer solution, enters the spiral pipe 521 through the second liquid inlet pipe 54 and the second connecting branch pipe 523, and the first liquid 70 and the second liquid 80 are uniformly mixed at the spiral pipe 521 to form a mixed solution and flow into the reagent carrying mechanism 51. After the second pump 552 is opened for a second predetermined time, the controller 561 controls the second pump 552 to close. The above steps are alternately executed, so that the first liquid 70 and the second liquid 80 are alternately sucked into the spiral pipe 521, until the adding amount of the first liquid 70 reaches the first preset sucking amount, and the adding amount of the second liquid 80 reaches the second preset sucking amount.
In one embodiment, the first pump body 551 and the second pump body 552 are both peristaltic pumps. In another embodiment, the first pump body 551 and the second pump body 552 are both liquid pumps.
Referring to fig. 9 and 10, in some embodiments, the control mechanism 56 further includes a first valve 564 electrically connected to the controller 561 and a second valve 565 electrically connected to the controller 561. The first valve 564 has a first port 5641, a second port 5642 and a third port 5643, the first port 5641 of the first valve 564 is connected to the inlet end of the first connecting branch 522, the second port 5642 of the first valve 564 is connected to the first inlet pipe 53, the third port 5643 of the first valve 564 is connected to the driving mechanism 55, and the third port 5643 of the first valve 564 is controllably communicated with the first port 5641 or the second port 5642 of the first valve 564.
Referring to fig. 9, the second valve 565 has a first opening 5651, a second opening 5652 and a third opening 5653, the first opening 5651 of the second valve 565 is connected to the inlet end of the second branch connecting pipe 523, the second opening 5652 of the second valve 565 is connected to the second inlet pipe 54, the third opening 5653 of the second valve 565 is connected to the driving mechanism 55, and the third opening 5653 of the second valve 565 is controllably communicated with the first opening 5651 or the second opening 5652 of the second valve 565.
Referring to fig. 9 and 10, in one embodiment, the driving mechanism 55 includes a first syringe 553, a first motor, a second syringe 554 and a second motor, and both the first motor and the second motor are electrically connected to the controller 561. The first syringe 553 is connected to the third port 5643 of the first valve 564 and the first motor is drivingly connected to the first syringe 553. The second syringe 554 is connected to the third opening 5653 of the second valve 565 and the second motor is drivingly connected to the second syringe 554.
Referring to fig. 9 and 10, in this embodiment, the controller 561 controls the third port 5643 of the first valve 564 to communicate with the second port 5642 of the first valve 564, and activates the first motor and controls the rotation direction of the first motor, so that the first syringe 553 sucks the first liquid 70 through the first liquid inlet pipe 53. After the first liquid inlet pipe 53 sucks the first liquid 70 for a first preset sucking time, the controller 561 controls the third port 5643 of the first valve 564 to communicate with the first port 5641 of the first valve 564, and changes the rotation direction of the first motor, so that the first liquid 70 in the first syringe 553 is injected into the spiral pipe 521 through the first connecting branch pipe 522, and the first liquid 70 and the second liquid 80 are uniformly mixed at the spiral pipe 521 to form a mixed liquid and flow into the reagent carrying mechanism 51.
After the first liquid 70 in the first syringe 553 is injected into the solenoid 521, the controller 561 controls the first motor to close and controls the third opening 5653 of the second valve 565 to communicate with the second opening 5652 of the second valve 565. The second motor is activated and the direction of rotation of the second motor is controlled such that the second syringe 554 draws the second liquid 80 through the second inlet tube 54. After the first liquid inlet pipe 53 sucks the second liquid 80 for a second preset sucking time, the controller 561 controls the third opening 5653 of the second valve 565 to be communicated with the first opening 5651 of the second valve 565, and changes the rotation direction of the first motor, so that the second liquid 80 in the second injector 554 is injected into the spiral pipe 521 through the second connecting branch pipe 523, and the first liquid 70 and the second liquid 80 are uniformly mixed through the spiral pipe 521 to form a mixed liquid and flow into the reagent carrying mechanism 51. After the second liquid 80 in the second syringe 554 is injected into the solenoid 521, the controller 561 controls the second motor to be turned off.
The above steps are alternately executed, so that the first liquid 70 and the second liquid 80 are alternately sucked into the spiral pipe 521, until the adding amount of the first liquid 70 reaches the first preset sucking amount, and the adding amount of the second liquid 80 reaches the second preset sucking amount.
Referring to fig. 11 and 13, in some embodiments, the turbulator structure 52 includes a first connection sub-tube 524, a spiral sub-tube 525, and a second connection sub-tube 526. One end of the first connection sub-tube 524 is connected to the reagent holding mechanism 51. One end of the spiral sub-tube 525 is connected to the other end of the first connection sub-tube 524.
Referring to fig. 11 and 13, one end of the second connection sub-pipe 526 is connected to the other end of the spiral sub-pipe 525, and the other end of the second connection sub-pipe 526 is connected to the first and second liquid inlet pipes 53 and 54. The drive mechanism 55 is provided on the first connection sub-pipe 524 or the second connection sub-pipe 526. Specifically, in one embodiment, the driving mechanism 55 is disposed on the second connection sub-tube 526, in which case the first connection sub-tube 524 may be omitted and the end of the spiral sub-tube 525 away from the driving mechanism 55 is connected to the reagent carrying mechanism 51. Of course, in other embodiments, the driving mechanism 55 can be disposed on the first connecting sub-tube 524, in which case the second connecting sub-tube 526 can be omitted, and the end of the spiral sub-tube 525 away from the reagent carrying mechanism 51 can be connected to the first liquid inlet tube 53 and the second liquid inlet tube 54.
Referring to fig. 11-14, the control mechanism 56 includes an automatic valve 566 electrically connected to the controller 561. The automatic valve 566 has a first inlet 5661, a second inlet 5662, and an outlet 5663, wherein the first inlet 5661 is connected to the first liquid inlet pipe 53, the second inlet 5662 is connected to the second liquid inlet pipe 54, the outlet 5663 is connected to the second connector sub-pipe 526, and the outlet 5663 is controllably communicated with the first inlet 5661 or the second inlet 5662.
Referring to fig. 11 and 12, in one embodiment, the drive mechanism 55 is a peristaltic pump. The controller 561 controls the peristaltic pump to start. The controller 561 controls the operation of the automatic valve 566 such that the outlet 5663 of the automatic valve 566 is communicated with the first inlet 5661 of the automatic valve 566, thereby communicating the solenoid 521, the second connector sub-pipe 526 and the first fluid inlet pipe 53. At this time, under the driving of the peristaltic pump, the first liquid 70, i.e. the initial dyeing liquid, enters the spiral pipe 521 through the first liquid inlet pipe 53 and the second connecting sub-pipe 526.
After the outlet 5663 of the automatic valve 566 is communicated with the first inlet 5661 of the automatic valve 566 for a first preset time, the controller 561 controls the automatic valve 566 to operate such that the outlet 5663 of the automatic valve 566 is communicated with the second inlet 5662 of the automatic valve 566, thereby communicating the solenoid 521, the second connecting sub-pipe 526 and the second fluid inlet pipe 54. At this time, under the driving of the peristaltic pump, the second liquid 80, i.e. the buffer solution, enters the spiral pipe 521 through the second liquid inlet pipe 54 and the second connecting sub-pipe 526, and the first liquid 70 and the second liquid 80 are uniformly mixed at the spiral pipe 521 to form a mixed solution and flow into the reagent carrying mechanism 51. The controller 561 controls the peristaltic pump to turn off after the outlet 5663 of the automatic valve 566 is in communication with the second inlet 5662 of the automatic valve 566 for a second predetermined time. The above steps are alternately executed, so that the first liquid 70 and the second liquid 80 are alternately sucked into the spiral pipe 521, until the adding amount of the first liquid 70 reaches the first preset sucking amount, and the adding amount of the second liquid 80 reaches the second preset sucking amount.
Referring to fig. 13 and 14, in one embodiment, the control mechanism 56 further includes a control valve switch 567. The control valve switch 567 is provided on the second connector sub-pipe 526, and has a first port 5671, a second port 5672, and a third port 5673, the first port 5671 of the control valve switch 567 communicates with the spiral sub-pipe 525, the second port 5672 of the control valve switch 567 communicates with the outlet 5663 of the automatic valve 566, the third port 5673 of the control valve switch 567 is connected to the drive mechanism 55, and the third port 5673 of the control valve switch 567 controllably communicates with the first port 5671 or the second port 5672.
Specifically, the drive mechanism 55 includes a third syringe 555 and a third motor (not shown) electrically connected to the controller 561. The third injector 555 is connected to the third port 5673 of the control valve switch 567, and the third motor is drivingly connected to the third injector 555.
Referring to fig. 13 and 14, the controller 561 controls the outlet 5663 of the automatic valve 566 to communicate with the first inlet 5661 of the automatic valve 566, the third port 5673 of the control valve switch 567 to communicate with the second port 5672 of the control valve switch 567, and the third motor is activated, so that the third syringe 555 sucks the first liquid 70 through the first liquid inlet pipe 53. After the third syringe 555 sucks the first liquid 70 for a first preset sucking time, the controller 561 controls the third port 5673 of the control valve switch 567 to communicate with the first port 5671 of the control valve switch 567 and controls the third motor to change the working direction, so that the first liquid 70 in the third syringe 555 is injected into the spiral pipe 521.
After the first liquid 70 in the third syringe 555 is injected into the spiral pipe 521, the controller 561 controls the outlet 5663 of the automatic valve 566 to communicate with the second inlet 5662 of the automatic valve 566, controls the third port 5673 of the valve switch 567 to communicate with the second port 5672 of the valve switch 567, and switches the operation direction of the third motor, so that the third syringe 555 sucks the second liquid 80 through the second liquid inlet pipe 54. After the third syringe 555 sucks the second liquid 80 for a second preset sucking time, the controller 561 controls the third port 5673 of the control valve switch 567 to be communicated with the first port 5671 of the control valve switch 567 and controls the third motor to change the working direction, so that the second liquid 80 in the third syringe 555 is injected into the spiral pipe 521. The first liquid 70 and the second liquid 80 are uniformly mixed at the spiral pipe 521 to form a mixed liquid and flow into the reagent carrying mechanism 51. After the second liquid 80 in the third syringe 555 is injected into the spiral pipe 521, the controller 561 controls the third motor to be turned off. The above steps are alternately executed, so that the first liquid 70 and the second liquid 80 are alternately sucked into the spiral pipe 521, until the adding amount of the first liquid 70 reaches the first preset sucking amount, and the adding amount of the second liquid 80 reaches the second preset sucking amount.
An embodiment of the present application further provides a biological sample dyeing method, in which the biological sample is dyed by the above-mentioned slide dyeing machine. The biological sample includes, but is not limited to, microorganisms, blood, body fluids, and bone marrow fluid.
Referring to fig. 17, in some embodiments, the method for staining a biological sample includes steps S101 to S103.
S101, absorbing the first liquid with the first preset absorption amount and the second liquid with the second preset absorption amount into the turbulent flow structure.
The first liquid is different from the second liquid, and the first liquid can be an initial dyeing liquid, and the second liquid is a buffer liquid, wherein the initial dyeing liquid is a high-concentration dyeing liquid, and the mixed liquid is a liquid obtained by diluting and uniformly mixing the initial dyeing liquid with the buffer liquid. Of course, the first liquid and the second liquid may be any other suitable liquids different from each other, for example, the first liquid is a buffer liquid, and the second liquid is an initial dyeing liquid. In order to express the concept of the present application more clearly, the following explanation will be given by taking the first liquid as the initial dyeing liquid and the second liquid as the buffer liquid.
Specifically, when the control mechanism is matched with the driving mechanism to enable the first liquid inlet pipe to be communicated with the turbulent flow structure, the first liquid is sucked into the turbulent flow structure from the first liquid inlet pipe; when the control mechanism is matched with the driving mechanism to enable the second liquid inlet pipe to be communicated with the turbulent flow structure, the second liquid is sucked into the turbulent flow structure from the second liquid inlet pipe.
In some embodiments, the sucking the first liquid with the first preset sucking amount and the second liquid with the second preset sucking amount into the turbulent flow structure includes: and alternately sucking the first liquid and the second liquid into the turbulent flow structure until the adding amount of the first liquid reaches the first preset sucking amount and the adding amount of the second liquid reaches the second preset sucking amount. The mode that first liquid and second liquid are inhaled in turn can make first liquid and second liquid more even mixture in vortex structure department.
It is understood that, in other embodiments, the first liquid and the second liquid may be simultaneously or time-divisionally absorbed into the turbulent flow structure, as long as the adding amount of the first liquid reaches the first preset absorbing amount, and the adding amount of the second liquid reaches the second preset absorbing amount, which is not limited herein.
S102, disturbing the first liquid and the second liquid through the disturbing flow structure to obtain mixed liquid.
Wherein, the vortex structure has the runner of carrying liquid. The turbulent flow structure is a structure capable of changing the liquid flowing through part or all of the flow channels from laminar flow to turbulent flow. Different liquids flowing to the turbulent flow structure can be quickly disturbed and uniformly mixed after forming turbulent flow, so that different liquids in the turbulent flow uniformly-mixing flow channel are realized, and mixed liquid is obtained. The obtained mixed liquid is sent to the reagent bearing mechanism by the turbulent flow structure so as to dye the smear.
Specifically, the turbulent flow structure may be a tubular structure having a bent portion or a tubular structure having a spiral portion, or the like. When the turbulent flow structure is a tubular structure with a spiral part, the spiral part can be made of a pipeline with any suitable inner diameter and any suitable length and any suitable material, for example, a rubber pipe with an inner diameter of 3.2mm and a length of 2000mm is used for making the spiral part with a spiral diameter of 40 mm.
The first liquid and the second liquid are uniformly mixed under the action of the turbulent flow structure, so that mixed liquid is obtained.
And S103, staining the smear by adopting the mixed solution.
Specifically, the smear is prepared by smearing the biological sample on the slide by the slide pushing device of the slide pushing and dyeing machine. The mixed liquid uniformly mixed by the turbulent flow structure flows out of the turbulent flow structure to the reagent bearing mechanism, and the smear is dyed by the mixed liquid in the reagent bearing mechanism, so that the dyeing of the biological sample on the picture is completed.
In some embodiments, before the sucking the first liquid into the flow disturbing structure, the method further includes: inputting a target ratio of the first liquid to the second liquid; and displaying a prestained graph of the biological sample under the target ratio.
Specifically, the target ratio of the first liquid to the second liquid can be set to any suitable ratio according to actual requirements, and a user can directly input the target ratio of the first liquid to the second liquid, or can set the target ratio by pulling a scroll bar and the like. The pre-staining graph of the biological sample in the target ratio is displayed, so that a user can know the pre-staining effect in the target ratio in advance, the pre-staining effect can be known in advance without trying to stain the user, and the proportion of the first liquid and the second liquid can be designed directly as required.
Specifically, the ratio input module is electrically connected with a controller of the control mechanism, and the target ratio of the first liquid and the second liquid is input through the ratio input module. And displaying a pre-staining image of the biological sample in a target ratio by a staining pre-display module.
According to the biological sample dyeing method, the first liquid and the second liquid are uniformly mixed at the turbulent flow structure, the mixed liquid obtained after uniform mixing flows into the reagent bearing mechanism to dye the biological sample, the mixed liquid which is uniformly mixed can be prepared from the initial dyeing liquid and the buffer liquid in any proportion and is input into the reagent bearing mechanism to dye, the mixed liquid is simple to prepare, the requirement on the flow speed of the liquid adding liquid is low, and compared with the air path driving mechanism with the high requirement on the flow speed of the liquid adding liquid, the raw material cost of the driving mechanism is reduced.
Various other modifications and changes may be made by those skilled in the art based on the above-described technical solutions and concepts, and all such modifications and changes should fall within the scope of the claims of the present application.
Claims (20)
1. A biological specimen staining apparatus, comprising:
the reagent bearing mechanism is used for storing the mixed liquid;
the turbulent flow structure is provided with a flow channel for conveying liquid, is used for turbulent flow and uniformly mixing the liquid in the flow channel, and conveys the mixed liquid to the reagent bearing mechanism;
the first liquid inlet pipe is connected to the turbulent flow structure and used for conveying first liquid;
the second liquid inlet pipe is connected to the turbulence structure in parallel with the first liquid inlet pipe and is used for conveying a second liquid;
the driving mechanism is arranged between the turbulence structure and the first liquid inlet pipe, arranged between the turbulence structure and the second liquid inlet pipe and used for providing liquid inlet power for the first liquid and the second liquid;
and the control mechanism is electrically connected with the driving mechanism to control the first liquid inlet pipe or the second liquid inlet pipe to be communicated with the turbulent flow structure.
2. The apparatus of claim 1, wherein the flow perturbation structure comprises:
one end of the spiral pipe is connected with the reagent bearing mechanism;
the first connecting branch pipe is provided with a liquid inlet end and a liquid outlet end, the liquid inlet end is connected to the first liquid inlet pipe, and the liquid outlet end is connected to the other end of the spiral pipe;
a second connecting branch pipe having an inlet end connected to the second liquid inlet pipe and an outlet end connected to the other end of the spiral pipe;
the driving mechanism is arranged between the first connecting branch pipe and the first liquid inlet pipe, and the driving structure is arranged between the second connecting branch pipe and the second liquid inlet pipe.
3. The apparatus according to claim 2, wherein the spiral tube extends in a transport direction of the liquid to form a spiral passage.
4. The device for staining biological samples according to claim 2, wherein the driving mechanism is a dual-channel peristaltic pump comprising:
the first connecting branch pipe penetrates through the first channel and is connected with the first liquid inlet pipe;
and the second connecting branch pipe penetrates through the second channel and is connected with the second liquid inlet pipe.
5. The device for staining biological samples according to claim 4, wherein the control mechanism comprises:
the control valve is provided with a first valve port, a second valve port and a third valve port, the first valve port is connected with the liquid outlet end of the first connecting branch pipe, the second valve port is connected with the outlet end of the second connecting branch pipe, the third valve port is connected with the spiral pipe, and the third valve port is controllably communicated with the first valve port or the second valve port;
and the controller is electrically connected with the driving mechanism and the control valve.
6. The device for staining biological samples according to claim 4, wherein the control mechanism comprises:
the first regulating valve is arranged on the first connecting branch pipe;
the second regulating valve is arranged on the second connecting branch pipe;
and the controller is electrically connected with the driving mechanism, the first regulating valve and the second regulating valve.
7. The device for staining a biological specimen according to claim 2, wherein the driving mechanism comprises:
the first pump body is arranged between the first connecting branch pipe and the first liquid inlet pipe;
and the second pump body is arranged between the second connecting branch pipe and the second liquid inlet pipe.
8. The apparatus according to claim 2, wherein the control mechanism comprises:
the first valve is provided with a first port, a second port and a third port, the first port is connected with the liquid inlet end of the first connecting branch pipe, the second port is connected with the first liquid inlet pipe, the third port is connected with the driving mechanism, and the third port is controllably communicated with the first port or the second port;
the second valve is provided with a first opening, a second opening and a third opening, the first opening is connected with the inlet end of the second connecting branch pipe, the second opening is connected with the second liquid inlet pipe, the third opening is connected with the driving mechanism, and the third opening is controllably communicated with the first opening or the second opening;
and the controller is electrically connected with the first valve, the second valve and the driving mechanism.
9. The device for staining biological samples according to claim 8, wherein the driving mechanism comprises:
a first injector connected to the third port of the first valve;
the first motor is in driving connection with the first injector and is electrically connected with the controller;
a second syringe connected to the third opening of the second valve;
and the second motor is in driving connection with the second injector and is electrically connected with the controller.
10. The apparatus of claim 1, wherein the flow perturbation structure comprises:
one end of the first connecting sub-pipe is connected with the reagent bearing mechanism;
one end of the spiral sub-pipe is connected with the other end of the first connecting sub-pipe;
one end of the second connecting sub-pipe is connected with the other end of the spiral sub-pipe, and the other end of the second connecting sub-pipe is connected with the first liquid inlet pipe and the second liquid inlet pipe; the driving mechanism is arranged on the first connecting sub-pipe or the second connecting sub-pipe.
11. The device for staining biological samples according to claim 10, wherein the control mechanism comprises:
the automatic valve is provided with a first inlet, a second inlet and an outlet, the first inlet is connected with the first liquid inlet pipe, the second inlet is connected with the second liquid inlet pipe, the outlet is connected with the second connecting sub pipe, and the outlet is controllably communicated with the first inlet or the second inlet;
and the controller is electrically connected with the driving mechanism and the automatic valve.
12. The device for staining a biological specimen according to claim 11, wherein the driving mechanism is a peristaltic pump.
13. The device for staining biological samples according to claim 11, wherein the control mechanism further comprises:
the control valve switch is arranged on the second connecting sub-pipe and is provided with a first interface, a second interface and a third interface, the first interface is communicated with the spiral sub-pipe, the second interface is communicated with the outlet of the automatic valve, the third interface is connected with the driving mechanism, and the third interface is controllably communicated with the first interface or the second interface.
14. The device for staining biological samples according to claim 13, wherein the driving mechanism comprises:
the third injector is connected with a third interface of the control valve switch;
and the third motor is in driving connection with the third injector and is electrically connected with the controller.
15. The apparatus for staining biological samples according to any of claims 1 to 14, wherein the apparatus further comprises:
the proportioning input module is electrically connected with the control mechanism and used for inputting the target proportioning of the first liquid and the second liquid;
and the dyeing pre-display module is electrically connected with the control mechanism and is used for displaying a pre-dyeing image of the biological sample in the target ratio.
16. The apparatus for staining biological samples according to any of claims 1 to 14, wherein the apparatus further comprises:
the first liquid supply container is communicated with the first liquid inlet pipe and is used for storing the first liquid;
and the second liquid supply container is communicated with the second liquid inlet pipe and is used for storing the second liquid.
17. A push piece dyeing machine, comprising:
a slide loading device for loading a slide;
a sample application device for loading a biological sample onto the slide;
the slide pushing device is used for flattening the biological sample on the slide to make a smear;
the biological sample staining apparatus of any one of claims 1-16, for staining the smear.
18. A method of staining a biological specimen, comprising:
absorbing a first liquid with a first preset absorption amount and a second liquid with a second preset absorption amount into the turbulent flow structure;
disturbing the first liquid and the second liquid by the disturbing structure to obtain a mixed liquid;
and staining the smear by using the mixed solution.
19. The method of claim 18, wherein prior to the step of drawing the first liquid into the turbulent flow structure, the method further comprises:
inputting a target ratio of the first liquid to the second liquid;
and displaying a prestained graph of the biological sample under the target ratio.
20. The method of claim 18, wherein the sucking a first liquid with a first preset sucking amount and a second liquid with a second preset sucking amount into the turbulent flow structure comprises:
and alternately sucking the first liquid and the second liquid into the turbulent flow structure until the adding amount of the first liquid reaches the first preset sucking amount and the adding amount of the second liquid reaches the second preset sucking amount.
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Cited By (1)
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| CN116106488A (en) * | 2023-04-13 | 2023-05-12 | 深圳市帝迈生物技术有限公司 | Sample analyzer |
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| 景朝晖 主编: "热工理论及应用", 28 February 2006, 中国电力出版社, pages: 160 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116106488A (en) * | 2023-04-13 | 2023-05-12 | 深圳市帝迈生物技术有限公司 | Sample analyzer |
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