CN105092342A - Blood smear staining method and blood smear staining box - Google Patents
Blood smear staining method and blood smear staining box Download PDFInfo
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- CN105092342A CN105092342A CN201410177596.2A CN201410177596A CN105092342A CN 105092342 A CN105092342 A CN 105092342A CN 201410177596 A CN201410177596 A CN 201410177596A CN 105092342 A CN105092342 A CN 105092342A
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- box body
- blood
- blood film
- box
- blood smear
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Abstract
The invention relates to the technical field of medical instruments, and provides a blood smear staining method and a blood smear staining box. The blood smear staining method comprises the following steps: a, putting a blood smear in a box; b, injecting a cell pre-immobilization staining solution into the box so as to perform cell pre-immobilization on the blood smear; c, discharging the cell pre-immobilization staining solution in the box; d, blowing air into the box to dry the blood smear; and e, injecting a staining solution into the box to stain the blood smear. The provided blood smear staining box comprises a box body for accommodating the cell pre-immobilization staining solution, the staining solution or clear water and also for accommodating the blood smear, an injection needle for injecting the cell pre-immobilization solution, the staining solution or clear water, a liquid-discharging needle for discharging the liquid in the box body, and an air-blowing needle for blowing air into the box body to dry the blood smear. The blood smear staining method is capable of simplifying the structure of the staining device, reducing staining flow and also improving the staining efficiency.
Description
Technical field
The present invention relates to technical field of medical instruments, be specifically related to blood film colouring method and dying box for blood smear.
Background technology
In push jack overflow dyeing machine, blood film generally adopts vertical colouring method, first pre-fixes dye liquor (methyl alcohol or using methyl alcohol as the dye liquor of solvent) with cell and carries out cell to blood film and pre-fix, dye after carrying out cell and pre-fixing to blood film; For reaching better dyeing quality, blood film needs to carry out drying after carrying out cell and pre-fixing, before dyeing.
The method dyeed to blood film that prior art provides need adopt mechanical mechanism to be taken out from colouration box by blood film then to adopt air-dry mechanism to carry out dry action to blood film, the structure of its device and the flow process of dyeing complicated.
Summary of the invention
The object of the invention is to provide blood film colouring method, is intended to the structure of simplification dyeing apparatus, reduces the flow process of dyeing, improves the efficiency of dyeing simultaneously.
For this reason, the present invention is by the following technical solutions:
Blood film colouring method, is characterized in that, comprises the following steps:
A. blood film is put into box body;
B. inject cell to pre-fix dye liquor and pre-fix to carry out cell to blood film to box body;
C. the cell in box body is pre-fixed dye liquor to discharge;
D. to blowing with dry blood film in box body;
E. dyeing dye liquor is injected to box body to dye to blood film.
Blood film colouring method provided by the invention, can simplify the structure of dyeing apparatus, reduces the flow process of dyeing, improve the efficiency of dyeing simultaneously.
Another object of the present invention is to provide dying box for blood smear, comprising:
Box body, pre-fixes dye liquor, dyeing dye liquor or clear water, simultaneously for accommodating blood film for accommodating cell;
Injection needle, pre-fixes liquid, dyeing dye liquor or clear water to described box body for injecting cell;
Discharge opeing pin, for discharging the liquid in described box body;
Blowing needle, for blowing in described box body with the described blood film of drying.
Dying box for blood smear provided by the invention, can simplify the structure of dyeing apparatus, reduces the flow process of dyeing, improve the efficiency of dyeing simultaneously.
Accompanying drawing explanation
The perspective view of the dying box for blood smear that Fig. 1 provides for the embodiment of the present invention;
Fig. 2 is equipped with the structural representation of blood film for dying box for blood smear that the embodiment of the present invention provides;
Fig. 3 is the structural representation of blood film;
Fig. 4 is the cross-sectional view of Fig. 2.
Embodiment
Below in conjunction with the drawings and the specific embodiments, the invention will be further described.
The blood film colouring method that the present embodiment provides, comprises the following steps:
A. blood film is put into box body;
B. inject cell to pre-fix dye liquor (methyl alcohol or using methyl alcohol as the dye liquor of solvent) to box body and pre-fix to carry out cell to blood film;
C. the cell in box body is pre-fixed dye liquor to discharge;
D. to blowing with dry blood film in box body;
E. dyeing dye liquor is injected to box body to dye to blood film.
In the present embodiment, described step e is specially:
E1. dye liquor A is injected to box body to dye to blood film;
E2. the dye liquor A in box body is discharged;
E3. inject mixed liquor to box body to dye to blood film, described mixed liquor is the mixed liquor of dye liquor A and damping fluid;
E4. the mixed liquor in box body is discharged;
E5. injected clear water to box body to rinse blood film;
E6. the clear water in box body is discharged;
E7. dyeed.
By upper, the blood film colouring method that the present embodiment provides carries out dry action without the need to adopting mechanical mechanism to blood film, simplifies the structure of dyeing apparatus, decreases the flow process of dyeing;
In addition, when blood film being carried out drying from box body taking-up in prior art, need to adopt container to collect the dye liquor that blood film in dry run flows down, and the blood film colouring method that the present embodiment provides, it directly carries out blow dried to blood film in box body, thus, dye liquor in this air blowing process on blood film flows directly in box body, this dye liquor is discharged by box body again, therefore, has further facilitated the structure simplifying dyeing apparatus, has improve the efficiency of dyeing simultaneously.
As further improvement of this embodiment, for discharging the dye liquor in air blowing process, blood film 2 flowing directly to box body 1 li, the blood film colouring method that the present embodiment provides, in Step d, carries out step c simultaneously.
With reference to Fig. 1,2,4.
The present embodiment additionally provides dying box for blood smear, comprising:
Box body 1, pre-fixes dye liquor, dyeing dye liquor or clear water, simultaneously for accommodating blood film 2 for accommodating cell;
Injection needle 5, pre-fixes liquid, dyeing dye liquor or clear water to described box body 1 for injecting cell;
Discharge opeing pin 3, for discharging the liquid in described box body 1;
Blowing needle 4, for blowing in described box body 1 with the described blood film of drying.
The dying box for blood smear that the present embodiment provides, it is as follows to the colouring method step of blood film:
A. blood film 2 is put into box body 1;
B. inject cell by injection needle 5 to pre-fix dye liquor (methyl alcohol or using methyl alcohol as the dye liquor of solvent) to box body 1 and pre-fix to carry out cell to blood film 2;
C. by discharge opeing pin 3, the cell in box body 1 is pre-fixed dye liquor to discharge;
D. by blowing with dry blood film 2 in blowing needle 4 pairs of box bodys 1;
E. inject dyeing dye liquor by injection needle 5 to dye to blood film 2.
In the present embodiment, step e is specially:
E1. dye liquor A is injected to box body 1 to dye to blood film 2 by injection needle 5;
E2. by discharge opeing pin 3, the dye liquor A in box body 1 is discharged;
E3. inject mixed liquor to box body to dye to blood film 2 by injection needle 5, described mixed liquor is the mixed liquor of dye liquor A and damping fluid;
E4. by discharge opeing pin 3, the mixed liquor in box body 1 is discharged;
E5. by injection needle 5 injected clear water to box body 1 to rinse blood film 2;
E6. by discharge opeing pin 3, the clear water in box body 1 is discharged;
E7. dyeed.
By upper, the dying box for blood smear that the present embodiment provides carries out dry action without the need to adopting mechanical mechanism to blood film, saves the structure of device, decreases the flow process of dyeing simultaneously;
In addition, when blood film being carried out drying from box body taking-up in prior art, need to adopt container to collect the dye liquor that blood film in dry run flows down, and the dying box for blood smear that the present embodiment provides, it directly carries out blow dried to blood film 2 in box body 1, thus, dye liquor in this air blowing process on blood film 2 flows directly to box body 1 li, this dye liquor is discharged by box body 1 again, therefore, has further facilitated the structure simplifying dyeing apparatus, has improve the efficiency of dyeing simultaneously.
For discharging the dye liquor in air blowing process, blood film 2 flowing directly to box body 1 li, this dying box for blood smear is carrying out, in Step d, carrying out step c simultaneously.
In the present embodiment, be provided with in box body 1 for carrying out spacing bottom support platform 11, right support platform 12 to blood film.
In the present embodiment, injection needle 5, discharge opeing pin 3, blowing needle 4 are respectively three pins; And in other embodiments, in described three pins, any two pins or three pins can be same pin, it all belongs to scope.
In other embodiments, blowing needle 4 also can be used as getter action, and it all belongs to scope.
Simultaneously with reference to Fig. 3, the carrier of blood film 2 is microslide, and national standard has carried out relevant regulations to the size of microslide: length 75mm ~ 76mm, width 25mm ~ 26mm, thickness 0.9mm ~ 1.1mm.
After blood film 2 is made, it comprises label area 21 and Xue Mo district 22, center, Xue Mo district 22 is as shown in dotted line circle in Fig. 3, be defined as to the shift of area that red blood cell scatters completely from the red blood cell overlapping area of about 50%, and lose central olistherozone with red blood cell, region that D structure does not see is terminating point.
Thus, blood film 2 is of a size of corresponding aspect after making: A >=25mm, C >=10mm, 28mm≤D≤38mm.
Therefore, for obtaining better drying effect, in the present embodiment, the inflatable mouth of blowing needle 4 is arranged in box body 1; The direction of the inflatable mouth 41 of blowing needle 4 is the side direction of blood film 2, the center line of inflatable mouth 41 blood film 2 and the angle β of vertical direction be 40 ~ 50 °, be 18 ~ 20mm with the vertical range L of the center line of blood film 2, be 51 ~ 54mm with the vertical range H of the bottom of blood film 2.
In the present embodiment, box body 1 inner bottom part is provided with spigot surface 13, is positioned at bottom spigot surface 13 and is provided with intercepting basin 14;
The leakage fluid dram 31 of discharge opeing pin 3 is arranged in intercepting basin 14.
This spigot surface 13 arranged and intercepting basin 14, can carry out discharge opeing to the liquid in box body 1 more fast and effectively.
In the present embodiment, dying box for blood smear also comprises pin fixed block 6, for fixing injection needle 5, discharge opeing pin 3 and blowing needle 4.
Can conveniently this injection needle 5, discharge opeing pin 3 and blowing needle 4 be fixed on external device (ED) by this pin fixed block 6; In other embodiments, also can arrange on box body 1 and fixedly be not used in fixing injection needle 5, discharge opeing pin 3 and blowing needle 4, no matter which kind of fixed form all should belong to scope.
More than for the present invention illustrates, be not limited to the present invention.
Claims (8)
1. blood film colouring method, is characterized in that, comprises the following steps:
A. blood film is put into box body;
B. inject cell to pre-fix dye liquor and pre-fix to carry out cell to blood film to box body;
C. the cell in box body is pre-fixed dye liquor to discharge;
D. to blowing with dry blood film in box body;
E. dyeing dye liquor is injected to box body to dye to blood film.
2. blood film colouring method as claimed in claim 1, it is characterized in that, described step e is specially:
E1. dye liquor A is injected to box body to dye to blood film;
E2. the dye liquor A in box body is discharged;
E3. inject mixed liquor to box body to dye to blood film, described mixed liquor is the mixed liquor of dye liquor A and damping fluid;
E4. the mixed liquor in box body is discharged;
E5. injected clear water to box body to rinse blood film;
E6. the clear water in box body is discharged;
E7. dyeed.
3. blood film colouring method as claimed in claim 1, is characterized in that, in described Step d, carry out step c simultaneously.
4. dying box for blood smear, is characterized in that, comprising:
Box body (1), pre-fixes dye liquor, dyeing dye liquor or clear water, simultaneously for accommodating blood film (2) for accommodating cell;
Injection needle (5), pre-fixes liquid, dyeing dye liquor or clear water to described box body (1) for injecting cell;
Discharge opeing pin (3), for discharging the liquid in described box body (1);
Blowing needle (4), for blowing in described box body (1) with the described blood film of drying.
5. dying box for blood smear as claimed in claim 4, is characterized in that, be provided with for carrying out spacing bottom support platform (11), right support platform (12) to described blood film in described box body (1).
6. dying box for blood smear as claimed in claim 4, it is characterized in that, the inflatable mouth of described blowing needle (4) is arranged in described box body (1); The direction of the inflatable mouth (41) of described blowing needle (4) is the side direction of described blood film (2), the angle β of the center line of described inflatable mouth (41) and described blood film (2) is 40 ~ 50 °, be 18 ~ 20mm with the vertical range L of the center line of described blood film (2), be 51 ~ 54mm with the vertical range H of the bottom of described blood film (2).
7. dying box for blood smear as claimed in claim 4, it is characterized in that, described box body (1) inner bottom part is provided with spigot surface (13), is positioned at described spigot surface (13) bottom and is provided with intercepting basin (14);
The leakage fluid dram (31) of described discharge opeing pin (3) is arranged in described intercepting basin (13).
8. dying box for blood smear as claimed in claim 4, it is characterized in that, described dying box for blood smear also comprises pin fixed block (6), for fixing described injection needle (5), described discharge opeing pin (3) and described blowing needle (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410177596.2A CN105092342B (en) | 2014-04-28 | 2014-04-28 | Blood film colouring method and dying box for blood smear |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410177596.2A CN105092342B (en) | 2014-04-28 | 2014-04-28 | Blood film colouring method and dying box for blood smear |
Publications (2)
| Publication Number | Publication Date |
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| CN105092342A true CN105092342A (en) | 2015-11-25 |
| CN105092342B CN105092342B (en) | 2019-10-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201410177596.2A Active CN105092342B (en) | 2014-04-28 | 2014-04-28 | Blood film colouring method and dying box for blood smear |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112113822A (en) * | 2019-06-21 | 2020-12-22 | 深圳迈瑞生物医疗电子股份有限公司 | Biological sample dyeing device, push piece dyeing machine and biological sample dyeing method |
| WO2020258155A1 (en) * | 2019-06-27 | 2020-12-30 | 深圳迈瑞生物医疗电子股份有限公司 | Slide maker and control method therefor |
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| JP2001021468A (en) * | 1999-07-09 | 2001-01-26 | Nippon Koden Corp | Method and apparatus for staining blood smear sample |
| US6268208B1 (en) * | 1998-02-18 | 2001-07-31 | Sysmex Corporation | Drying configuration and method in smear specimen preparation system |
| CN1916626A (en) * | 2006-09-06 | 2007-02-21 | 江西特康科技有限公司 | Substrate for blood smear, and apparatus of blood smear |
| CN102095621A (en) * | 2011-01-13 | 2011-06-15 | 上海斯而杰医疗器械技术有限公司 | Tilting blood smear dyeing box |
| CN102192852A (en) * | 2010-03-11 | 2011-09-21 | 希森美康株式会社 | Smear staining apparatus, smear processing system, and method for determining staining condition |
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2014
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| US6268208B1 (en) * | 1998-02-18 | 2001-07-31 | Sysmex Corporation | Drying configuration and method in smear specimen preparation system |
| JP2001021468A (en) * | 1999-07-09 | 2001-01-26 | Nippon Koden Corp | Method and apparatus for staining blood smear sample |
| CN1916626A (en) * | 2006-09-06 | 2007-02-21 | 江西特康科技有限公司 | Substrate for blood smear, and apparatus of blood smear |
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Cited By (2)
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| CN112113822A (en) * | 2019-06-21 | 2020-12-22 | 深圳迈瑞生物医疗电子股份有限公司 | Biological sample dyeing device, push piece dyeing machine and biological sample dyeing method |
| WO2020258155A1 (en) * | 2019-06-27 | 2020-12-30 | 深圳迈瑞生物医疗电子股份有限公司 | Slide maker and control method therefor |
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| Publication number | Publication date |
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| CN105092342B (en) | 2019-10-11 |
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Application publication date: 20151125 Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Contract record no.: X2022440020009 Denomination of invention: Blood smear staining method and blood smear staining box Granted publication date: 20191011 License type: Common License Record date: 20220804 |