CN105092342B - Blood film colouring method and dying box for blood smear - Google Patents
Blood film colouring method and dying box for blood smear Download PDFInfo
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- CN105092342B CN105092342B CN201410177596.2A CN201410177596A CN105092342B CN 105092342 B CN105092342 B CN 105092342B CN 201410177596 A CN201410177596 A CN 201410177596A CN 105092342 B CN105092342 B CN 105092342B
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Abstract
The present invention relates to the field of medical instrument technology, provide blood film colouring method and dying box for blood smear.Provided blood film colouring method is the following steps are included: blood film is put into box body by a.；B. injection cell pre-fixes dye liquor and is pre-fixed to box body with carrying out cell to blood film；C. the intracorporal cell of box is pre-fixed into dye liquor discharge；D. to being blown in box body to dry blood film；E. injection dyeing dye liquor is to box body to dye to blood film.There is provided dying box for blood smear includes: box body, pre-fixes dye liquor, dyeing dye liquor or clear water for accommodating cell, while for accommodating blood film；Injection needle pre-fixes liquid, dyeing dye liquor or clear water to box body for injecting cell；Drain needle, for the intracorporal liquid of box to be discharged；Blowing needle, for being blown in box body to dry blood film.The blood film colouring method can simplify the structure of dyeing apparatus, reduce the process of dyeing while improve the efficiency of dyeing.
Description
Technical field
The present invention relates to the field of medical instrument technology, and in particular to blood film colouring method and dying box for blood smear.
Background technique
In push jack overflow dyeing machine, blood film generally uses vertical colouring method, first with cell pre-fix dye liquor (methanol or with
Dye liquor of the methanol as solvent) blood film progress cell is pre-fixed, blood film is contaminated after carrying out cell and pre-fixing
Color；For that can reach better dyeing quality, blood film needs to be dried after carrying out cell and pre-fixing, before dyeing.
The method dyed to blood film that the prior art provides need to use mechanical mechanism by blood film from colouration box
It takes out and movement is then dried to blood film using air-dried mechanism, the structure of device and the process of dyeing are complicated.
Summary of the invention
It is an object of that present invention to provide blood film colouring methods, it is intended to simplify the structure of dyeing apparatus, reduce the stream of dyeing
Journey, the efficiency for improving dyeing simultaneously.
For this purpose, the invention adopts the following technical scheme:
Blood film colouring method, which comprises the following steps:
A. blood film is put into box body；
B. injection cell pre-fixes dye liquor and is pre-fixed to box body with carrying out cell to blood film；
C. the intracorporal cell of box is pre-fixed into dye liquor discharge；
D. to being blown in box body to dry blood film；
E. injection dyeing dye liquor is to box body to dye to blood film.
Blood film colouring method provided by the invention can simplify the structure of dyeing apparatus, reduce the process of dyeing while mention
The efficiency of height dyeing.
Another object of the present invention is to provide dying box for blood smear, comprising:
Box body pre-fixes dye liquor, dyeing dye liquor or clear water for accommodating cell, while for accommodating blood film；
Injection needle pre-fixes liquid, dyeing dye liquor or clear water to the box body for injecting cell；
Drain needle, for the intracorporal liquid of the box to be discharged；
Blowing needle, for being blown in the box body with the drying blood film.
Dying box for blood smear provided by the invention can simplify the structure of dyeing apparatus, reduce the process of dyeing while improve
The efficiency of dyeing.
Detailed description of the invention
Fig. 1 is the schematic perspective view of dying box for blood smear provided in an embodiment of the present invention；
Fig. 2 is the structural schematic diagram that dying box for blood smear provided in an embodiment of the present invention is equipped with blood film；
Fig. 3 is the structural schematic diagram of blood film；
Fig. 4 is the schematic diagram of the section structure of Fig. 2.
Specific embodiment
Below in conjunction with the drawings and the specific embodiments, the invention will be further described.
Blood film colouring method provided in this embodiment, comprising the following steps:
A. blood film is put into box body；
B. injection cell pre-fixes dye liquor (methanol or using methanol as the dye liquor of solvent) to box body to carry out to blood film
Cell pre-fixes；
C. the intracorporal cell of box is pre-fixed into dye liquor discharge；
D. to being blown in box body to dry blood film；
E. injection dyeing dye liquor is to box body to dye to blood film.
In the present embodiment, the step e specifically:
E1. injection dye liquor A is to box body to dye to blood film；
E2. the intracorporal dye liquor A of box is discharged；
E3. for injection mixed liquor to box body to dye to blood film, the mixed liquor is the mixing of dye liquor A and buffer
Liquid；
E4. the intracorporal mixed liquor of box is discharged；
E5. injected clear water is to box body to be rinsed to blood film；
E6. the intracorporal clear water of box is discharged；
E7. dyeing is completed.
By upper, blood film colouring method provided in this embodiment is dried blood film without mechanical mechanism dynamic
Make, simplifies the structure of dyeing apparatus, reduces the process of dyeing；
In addition, needing using container when blood film being dried from box body taking-up in the prior art in drying process
The dye liquor flowed down on blood film is collected, and blood film colouring method provided in this embodiment, directly to blood in box body
Smear carries out blow dried, and the dye liquor in the air blowing process on blood film flows directly in box body as a result, and the dye liquor is again by box body
Discharge, therefore, has further facilitated the structure for simplifying dyeing apparatus while the efficiency for improving dyeing.
As further improvement of this embodiment, to flow directly to the dye in box body 1 on blood film 2 in discharge air blowing process
Liquid, blood film colouring method provided in this embodiment in Step d, while carrying out step c.
Referring to Fig.1,2,4.
The present embodiment additionally provides dying box for blood smear, comprising:
Box body 1 pre-fixes dye liquor, dyeing dye liquor or clear water for accommodating cell, while for accommodating blood film 2；
Injection needle 5 pre-fixes liquid, dyeing dye liquor or clear water to the box body 1 for injecting cell；
Drain needle 3, for the liquid in the box body 1 to be discharged；
Blowing needle 4, for blowing in the box body 1 with the drying blood film.
Dying box for blood smear provided in this embodiment, to the colouring method of blood film, steps are as follows:
A. blood film 2 is put into box body 1；
B. by injection needle 5 inject cell pre-fix dye liquor (methanol or using methanol as the dye liquor of solvent) to box body 1 with
Cell is carried out to blood film 2 to pre-fix；
C. the cell in box body 1 is pre-fixed by dye liquor discharge by drain needle 3；
D. by blowing needle 4 to being blown in box body 1 to dry blood film 2；
E. blood film 2 is dyed by the injection dyeing dye liquor of injection needle 5.
In the present embodiment, step e specifically:
E1. dye liquor A is injected to box body 1 to dye to blood film 2 by injection needle 5；
E2. the dye liquor A in box body 1 is discharged by drain needle 3；
E3. by injection needle 5 inject mixed liquor to box body to be dyed to blood film 2, the mixed liquor be dye liquor A with
The mixed liquor of buffer；
E4. the mixed liquor in box body 1 is discharged by drain needle 3；
E5. pass through 5 injected clear water of injection needle to box body 1 to be rinsed to blood film 2；
E6. the clear water in box body 1 is discharged by drain needle 3；
E7. dyeing is completed.
By upper, movement is dried to blood film without mechanical mechanism in dying box for blood smear provided in this embodiment,
It saves the structure of device while reducing the process of dyeing；
In addition, needing using container when blood film being dried from box body taking-up in the prior art in drying process
The dye liquor flowed down on blood film is collected, and dying box for blood smear provided in this embodiment, is applied directly in box body 1 to blood
Piece 2 carries out blow dried, and the dye liquor in the air blowing process on blood film 2 flows directly in box body 1 as a result, and the dye liquor is again by box
Body 1 is discharged, and therefore, has further facilitated the structure for simplifying dyeing apparatus while the efficiency for improving dyeing.
To flow directly to the dye liquor in box body 1 on blood film 2 in discharge air blowing process, which is carrying out d
In step, while carrying out step c.
In the present embodiment, bottom supporting table 11 for being limited to blood film, right support platform are provided in box body 1
12。
In the present embodiment, injection needle 5, drain needle 3, blowing needle 4 are respectively three needles；And in other embodiments, it is described
Any two needles or three needles can be same root needle in three needles, belong to the scope of the present invention.
In other embodiments, blowing needle 4 also can be used as getter action, belong to the scope of the present invention.
Referring concurrently to Fig. 3, the carrier of blood film 2 is glass slide, and national standard has carried out phase to the size of glass slide
Close regulation: length 75mm~76mm, width 25mm~26mm, thickness 0.9mm~1.1mm.
After blood film 2 is made comprising in label area 21 and blood film area 22,22 center of blood film area such as Fig. 3
Shown in virtual coil, it is defined as the shift of area to scatter completely since about 50% red blood cell overlapping area to red blood cell,
And central olistherozone is lost using red blood cell, terminating point is in region that D structure is not seen.
The size of corresponding aspect after blood film 2 is made as a result, are as follows: A >=25mm, C >=10mm, 28mm≤D≤38mm.
It therefore, is the better drying effect of acquisition, in the present embodiment, the inflatable mouth of blowing needle 4 is set in box body 1；It blows
The direction of the inflatable mouth 41 of air tube 4 is lateral, the center line of 41 blood film 2 of inflatable mouth and the angle β of vertical direction of blood film 2
It is 18~20mm for 40~50 °, with the vertical range L of the center line of blood film 2, with the vertical range H of the bottom of blood film 2 is
51~54mm.
In the present embodiment, bottom is provided with guide surface 13 in box body 1, and 13 bottom of guide surface is provided with collecting tank 14；
The leakage fluid dram 31 of drain needle 3 is set in collecting tank 14.
The guide surface 13 and collecting tank 14 being arranged more rapidly, effectively can carry out drain to the liquid in box body 1.
In the present embodiment, dying box for blood smear further includes needle fixed block 6, for fixing injection needle 5, drain needle 3 and blowing
Air tube 4.
It can be facilitated by the needle fixed block 6 and the injection needle 5, drain needle 3 and blowing needle 4 are fixed on external device (ED)；
In other embodiments, fixation can be also set on box body 1 and be not used in fixed injection needle 5, drain needle 3 and blowing needle 4 no matter
Which kind of fixed form should belong to the scope of the present invention.
The above are the present invention for example, being not intended to restrict the invention.
Claims (8)
1. blood film colouring method, which comprises the following steps:
A. blood film is put into box body；
B. cell is injected by injection needle and pre-fixes dye liquor to box body to pre-fix to blood film progress cell；
C. the intracorporal cell of box is pre-fixed by dye liquor discharge by drain needle；
D. blood film is dried to blowing in box body by blowing needle, and the inflatable mouth of the blowing needle is set to the box
In vivo, the direction of the inflatable mouth of the blowing needle is lateral, the center of the inflatable mouth and the blood film of the blood film
The angle β of line is 40~50 °, and injection needle, drain needle, blowing needle enter in box body from the same entrance of box body；
E. dyeing dye liquor is injected to box body to dye to blood film by injection needle.
2. blood film colouring method as described in claim 1, which is characterized in that the step e specifically:
E1. injection dye liquor A is to box body to dye to blood film；
E2. the intracorporal dye liquor A of box is discharged；
E3. for injection mixed liquor to box body to dye to blood film, the mixed liquor is the mixed liquor of dye liquor A and buffer；
E4. the intracorporal mixed liquor of box is discharged；
E5. injected clear water is to box body to be rinsed to blood film；
E6. the intracorporal clear water of box is discharged；
E7. dyeing is completed.
3. blood film colouring method as described in claim 1, which is characterized in that in the Step d, while carrying out step c.
4. dying box for blood smear characterized by comprising
Box body (1) pre-fixes dye liquor, dyeing dye liquor or clear water for accommodating cell, while for accommodating blood film (2)；
Injection needle (5) pre-fixes liquid, dyeing dye liquor or clear water to the box body (1) for injecting cell；
Drain needle (3), for the liquid in the box body (1) to be discharged；
Blowing needle (4), for blowing to the box body (1) is interior with the drying blood film；
Wherein, the injection needle (5), drain needle (3), blowing needle (4) enter in box body (1) from the same entrance of box body (1)；
The inflatable mouth of the blowing needle (4) is set in the box body (1).
5. dying box for blood smear as claimed in claim 4, which is characterized in that be provided in the box body (1) for described
Bottom supporting table (11) that blood film is limited, right support platform (12).
6. dying box for blood smear as claimed in claim 4, which is characterized in that the side of the inflatable mouth (41) of the blowing needle (4)
To for the lateral of the blood film (2), the angle β of the center line of the inflatable mouth (41) and the blood film (2) is 40~
50 °, with the vertical range L of the center line of the blood film (2) be 18~20mm, vertical with the bottom of the blood film (2)
Distance H is 51~54mm.
7. dying box for blood smear as claimed in claim 4, which is characterized in that the interior bottom of the box body (1) is provided with guide surface
(13), it is located at the guide surface (13) bottom and is provided with collecting tank (14)；
The leakage fluid dram (31) of the drain needle (3) is set in the collecting tank (13).
8. dying box for blood smear as claimed in claim 4, which is characterized in that the dying box for blood smear further includes needle fixed block
(6), for fixing the injection needle (5), the drain needle (3) and the blowing needle (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410177596.2A CN105092342B (en) | 2014-04-28 | 2014-04-28 | Blood film colouring method and dying box for blood smear |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410177596.2A CN105092342B (en) | 2014-04-28 | 2014-04-28 | Blood film colouring method and dying box for blood smear |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105092342A CN105092342A (en) | 2015-11-25 |
| CN105092342B true CN105092342B (en) | 2019-10-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410177596.2A Active CN105092342B (en) | 2014-04-28 | 2014-04-28 | Blood film colouring method and dying box for blood smear |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112113822A (en) * | 2019-06-21 | 2020-12-22 | 深圳迈瑞生物医疗电子股份有限公司 | Biological sample dyeing device, push piece dyeing machine and biological sample dyeing method |
| WO2020258155A1 (en) * | 2019-06-27 | 2020-12-30 | 深圳迈瑞生物医疗电子股份有限公司 | Slide maker and control method therefor |
Citations (4)
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| US6268208B1 (en) * | 1998-02-18 | 2001-07-31 | Sysmex Corporation | Drying configuration and method in smear specimen preparation system |
| CN1916626A (en) * | 2006-09-06 | 2007-02-21 | 江西特康科技有限公司 | Substrate for blood smear, and apparatus of blood smear |
| CN102095621A (en) * | 2011-01-13 | 2011-06-15 | 上海斯而杰医疗器械技术有限公司 | Tilting blood smear dyeing box |
| CN102192852A (en) * | 2010-03-11 | 2011-09-21 | 希森美康株式会社 | Smear staining apparatus, smear processing system, and method for determining staining condition |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3620013B2 (en) * | 1999-07-09 | 2005-02-16 | 日本光電工業株式会社 | Method and apparatus for staining blood smear |
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2014
- 2014-04-28 CN CN201410177596.2A patent/CN105092342B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6268208B1 (en) * | 1998-02-18 | 2001-07-31 | Sysmex Corporation | Drying configuration and method in smear specimen preparation system |
| CN1916626A (en) * | 2006-09-06 | 2007-02-21 | 江西特康科技有限公司 | Substrate for blood smear, and apparatus of blood smear |
| CN102192852A (en) * | 2010-03-11 | 2011-09-21 | 希森美康株式会社 | Smear staining apparatus, smear processing system, and method for determining staining condition |
| CN102095621A (en) * | 2011-01-13 | 2011-06-15 | 上海斯而杰医疗器械技术有限公司 | Tilting blood smear dyeing box |
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Application publication date: 20151125 Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Contract record no.: X2022440020009 Denomination of invention: Blood smear staining method and blood smear staining box Granted publication date: 20191011 License type: Common License Record date: 20220804 |