CN112113814A - Exfoliative cell immunochemistry sheet making device and using method thereof - Google Patents
Exfoliative cell immunochemistry sheet making device and using method thereof Download PDFInfo
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- CN112113814A CN112113814A CN202010996245.XA CN202010996245A CN112113814A CN 112113814 A CN112113814 A CN 112113814A CN 202010996245 A CN202010996245 A CN 202010996245A CN 112113814 A CN112113814 A CN 112113814A
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- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000011521 glass Substances 0.000 claims abstract description 90
- 230000000984 immunochemical effect Effects 0.000 claims abstract description 18
- 230000002093 peripheral effect Effects 0.000 claims abstract description 5
- 239000002244 precipitate Substances 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 25
- 238000007789 sealing Methods 0.000 claims description 12
- 238000009434 installation Methods 0.000 claims description 10
- 230000000903 blocking effect Effects 0.000 claims description 8
- 239000006285 cell suspension Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 239000008280 blood Substances 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000008055 phosphate buffer solution Substances 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 229960000583 acetic acid Drugs 0.000 claims description 3
- 230000006978 adaptation Effects 0.000 claims description 3
- 239000012362 glacial acetic acid Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000011324 bead Substances 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 239000004576 sand Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 29
- 238000000926 separation method Methods 0.000 description 8
- 238000004321 preservation Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 5
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 210000000981 epithelium Anatomy 0.000 description 3
- 210000002865 immune cell Anatomy 0.000 description 3
- 238000003365 immunocytochemistry Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000007969 cellular immunity Effects 0.000 description 2
- 210000003679 cervix uteri Anatomy 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000521257 Hydrops Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000000244 kidney pelvis Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 210000003741 urothelium Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the technical field of sheet makers, and particularly relates to an exfoliative cell immunochemical sheet maker and a using method thereof, wherein the sheet maker comprises the following steps: the device comprises a slide carrier body, wherein two ends of the slide carrier body respectively extend downwards to form a limiting plate, one end of the lower surface of the slide carrier body is provided with two front retaining pieces, and the other end of the lower surface of the slide carrier body is provided with a rear retaining piece; the dropping mechanism comprises a mounting pipe fixedly communicated with the upper surface of the slide glass body, a dropping pipe communicated with the mounting pipe is mounted on the mounting pipe, and two clamping handles are symmetrically and fixedly connected to the peripheral wall of the dropping pipe; the special glass slide is clamped between the two limiting plates; the limiting mechanism comprises glass carrying clamping pieces clamped between the two limiting plates, and the glass carrying clamping pieces are located below the special glass slides and tightly attached to the special glass slides. The invention has the advantages of uniform cell distribution, proper thickness, easy observation, rapidness, convenience and easy operation, can be suitable for multiple centrifugal pelleters, and can be widely applied to hospitals at all levels.
Description
Technical Field
The invention belongs to the technical field of sheet makers, and particularly relates to an exfoliative cell immunochemical sheet maker and a using method thereof.
Background
The exfoliated cells refer to exfoliated cells in natural lumen organs, wherein the mucosal epithelial cells of human organs are frequently exfoliated and renewed under normal conditions of mucosa on the inner surfaces of the natural lumen organs, and diseased mucosal epithelial cells, such as vaginal epithelium, bronchial mucosal epithelium, renal pelvis bladder transitional epithelium, mammary duct epithelium and the like, are naturally exfoliated epithelial cells, and body cavity hydrops, such as exfoliated cells of thoracic cavity, abdominal cavity, pericardial cavity and the like. A chemical slide-making device for cast-off cell immune cells is suitable for the chemical detection of cytopathology immune cells such as body cavity effusion and the like and the chemical detection of cervix liquid-based cell immune cells in pathology departments of all levels of hospitals.
In the conventional immunochemical detection of the exfoliated cells, a detection sample is made into a cell block, and then the cell block is sliced by conventional paraffin to be made into an immunocytochemical sheet and other operation procedures. The special glass slide is directly clamped in the use process of the traditional slide maker, and the special glass slide is made of a thinner glass material, so that the special glass slide is easy to crack and damage in the clamping process of the special glass slide in the traditional slide maker, and smear cells are uneven; the non-special glass slide is easy to cause the shedding of smear cells, so that the slide making success rate is low.
Disclosure of Invention
The invention provides an exfoliative cell immunochemical slide making device and a use method thereof, aiming at solving the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: an exfoliated cell immunochemical slide maker comprising: the device comprises a slide carrier body, wherein two ends of the slide carrier body respectively extend downwards to form a limiting plate, one end of the lower surface of the slide carrier body is provided with two front retaining pieces, and the other end of the lower surface of the slide carrier body is provided with a rear retaining piece;
the dropping mechanism comprises a mounting pipe fixedly communicated with the upper surface of the slide glass body, a dropping pipe communicated with the mounting pipe is mounted on the mounting pipe, and two clamping handles are symmetrically and fixedly connected to the peripheral wall of the dropping pipe;
the special glass slide is clamped between the two limiting plates;
the limiting mechanism comprises glass carrying clamping pieces clamped between the two limiting plates, and the glass carrying clamping pieces are located below the special glass slides and tightly attached to the special glass slides.
Preferably, the opposite surfaces of the two limiting plates are respectively provided with a clamping groove, and two ends of the glass carrying clamping piece respectively extend downwards and are provided with spacing strips matched with the clamping grooves.
Preferably, the division bar is provided in a wedge shape.
Preferably, the dropping liquid mechanism still establishes including the cover the filter screen pipe of installation tube inner chamber, filter screen pipe top with dropwise add pipe integrated into one piece, filter screen pipe bottom fixedly connected with sealing ring, just be located on the carrier body the mounting groove has been seted up in the outside of installation pipe, the sealing ring cup joint in the mounting groove.
Preferably, two spacing grooves have been seted up to the symmetry on the mounting groove, sealing ring periphery wall fixedly connected with and two spacing pieces of spacing groove looks adaptation, two spacing piece joint respectively in two spacing inslot.
Preferably, a through finger groove is formed in the upper surface of the slide glass body and between the two front blocking pieces.
Preferably, the height of the front baffle is smaller than that of the rear baffle.
Preferably, the lower surface of the glass carrying clamping piece is provided with a through finger hole.
Preferably, carry glass clamping piece lower surface and be located indicate one side integrated into one piece in hole has a plurality of equidistance, the sand grip of evenly arranging, carry glass clamping piece lower surface and be located indicate the opposite side integrated into one piece in hole to have the lug.
A use method of an exfoliated cell immunochemical slide maker comprises the following steps:
step one, adopting a 50ml disposable pointed-bottom centrifuge tube, taking 50-100 ml of liquid at the bottom of a body cavity effusion container (1-2 centrifuge tubes per sample), centrifuging for 5 minutes at the rotating speed of 2500-3000 rpm, and discarding supernatant.
And step two, when the content of the precipitated blood is more than 1/2 of the precipitate, washing the precipitate for 5-10 minutes by 15-20ml of 10% glacial acetic acid, centrifuging the precipitate for 5 minutes at the rotation speed of 1200 rpm, washing the precipitate by Phosphate Buffer Solution (PBS), centrifuging the precipitate for 5 minutes at the rotation speed of 1200 rpm, and centrifuging the precipitate twice. This step can be omitted when the blood content is low.
And step three, when the amount of the precipitate is greater than 2ml, sucking 1/2 of the precipitate, placing the precipitate into a liquid-based storage bottle for fixation and other treatment, and when the amount is less than 2ml, placing all the precipitate into the liquid-based storage bottle.
Step four, 3ml of the cell suspension is added into the sheet making device, and then 2ml of cell cleaning solution is added;
and step five, placing the tabletting machine in a centrifugal tabletting machine, and centrifuging for 5 minutes at the rotating speed of 1200 rpm.
And step six, taking out the slide maker from the centrifugal slide maker, dumping liquid on the upper part of the slide maker, removing the limitation of the limiting mechanism in the slide maker, and taking out the special glass slide for natural drying or drying.
Compared with the prior art, the invention has the beneficial effects that: the special glass slide can be effectively pressed after the glass slide clamping piece is clamped between the two clamping grooves, so that the special glass slide is limited, and meanwhile, the front and rear blocking pieces can limit the glass slide clamping piece, so that the problem that the special glass slide is cracked and damaged in the process of being clamped into a traditional slide maker, and the slide making success rate is low is effectively solved; adopt this immunocytochemistry film-making ware, use ordinary centrifugation film-making machine can, do not need expensive full-automatic film-making equipment, can prepare cell immunocytochemistry piece fast, prepare liquid-based thin layer TCT smear simultaneously in the lump, not only economy but also swift simple and convenient, and immunocytochemistry P16 testing result is accurate reliable, is particularly useful for the inspection of cervix cytology double screen. The cell immunochemistry detection of the coelomic effusion can identify the cells in the coelomic effusion and judge the source of the tumor cells.
Drawings
FIG. 1 is a perspective view from below of the present invention;
FIG. 2 is a front view of the present invention;
FIG. 3 is a bottom view of the carrier body of the present invention;
FIG. 4 is a side view of a dropping tube of the present invention;
FIG. 5 is a bottom view of the spacing mechanism of the present invention;
in the figure: 1-slide piece body, 11-extension plate, 111-clamping groove, 12-front blocking piece, 13-rear blocking piece, 14-mounting groove, 15-finger groove, 16-limiting groove, 2-dropping liquid mechanism, 21-mounting tube, 22-dropping tube, 23-filter screen tube, 24-sealing ring, 241-limiting piece, 25-clamping handle, 3-special slide glass, 4-limiting mechanism, 41-slide glass clamping piece, 42-parting bead, 43-convex strip, 44-finger hole and 45-convex block.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution: an exfoliated cell immunochemical slide maker comprising: the device comprises a slide carrier body 1, wherein two ends of the slide carrier body 1 respectively extend downwards to form a limiting plate 11, one end of the lower surface of the slide carrier body 1 is provided with two front retaining pieces 12, and the other end of the lower surface of the slide carrier body 1 is provided with a rear retaining piece 13;
the dropping mechanism 2 comprises a mounting tube 21 fixedly communicated with the upper surface of the slide glass body 1, a dropping tube 22 communicated with the mounting tube 21 is mounted on the mounting tube 21, and two clamping handles 25 are symmetrically and fixedly connected to the outer peripheral wall of the dropping tube 22;
the special glass slide 3 is clamped between the two limiting plates 11;
the limiting mechanism 4 and the limiting mechanism 4 comprise glass carrying clamping pieces 41 which are clamped between the two limiting plates 11, and the glass carrying clamping pieces 41 are located below the special glass slides 3 and are tightly attached to the special glass slides.
In this embodiment, when the special slide 3 is mounted, the special slide 3 is first moved from the front end of the slide body 1 having the front stop piece 12 to the rear end of the slide body 1 having the rear stop piece 13, and the special glass slide 3 is clamped between the two limit plates 11, after the special glass slide 3 is moved and installed, the two limit plates 11 clamp the glass slide, the glass slide clamping piece 41 is operated according to the same steps of the special glass slide 3, the glass slide clamping piece 41 is clamped between the two limit plates 11, and glass slide clamping piece 41 is located the lower surface of special slide glass 3 and compresses tightly special slide glass 3 in order to restrict its removal, and glass slide clamping piece 41 is located between two preceding separation blades 12 and back separation blade 13, and two preceding separation blades 12 block glass slide clamping piece 41 with back separation blade 13, and dropping pipe 22 is used for the cell preservation liquid dropwise add of treating the film-making and carries out the film-making operation on special slide glass 3, and card handle 25 is used for placing this film-making ware on centrifugal film-making machine's film-making station fixedly. The dropping pipe 22 may be set to have an outer diameter of 1.5cm, an inner diameter of 1.3cm and a height of 3 cm. It should be noted that, one end of the slide piece body 1 close to the rear retaining piece 13 can be set to be an arc-shaped structure, so that the resistance of the slide piece device during centrifugal rotation can be reduced in the centrifugal slide making process of the centrifugal slide piece making machine.
Furthermore, the opposite surfaces of the two limiting plates 11 are respectively provided with a clamping groove 111, and two ends of the glass carrying clamping piece 41 respectively extend downwards to form a spacing strip 42 matched with the clamping groove 111.
In this embodiment, the two spacers 42 are respectively adapted to the two slots 111 in shape and in positions, and the two spacers 42 are slidably connected to the slots 111, so that the glass slide clip 41 is conveniently clamped between the two limiting plates 11.
Further, the division bars 42 are arranged in a wedge shape.
In this embodiment, the width from one end of the slot 111 to the other end of the slot can be gradually increased or decreased, and the division bar 42 is set to be a wedge-shaped structure matched with the shape of the slot 111, so that the glass clip 41 can be conveniently inserted into the inner cavity of the slot 111, and the special glass slide 3 can be conveniently locked and limited.
Further, dropping liquid mechanism 2 still establishes filter screen pipe 23 at installation pipe 21 inner chamber including the cover, and filter screen pipe 23 top and dropwise add pipe 22 integrated into one piece, filter screen pipe 23 bottom fixedly connected with sealing ring 24, and the installation groove 14 has been seted up in the outside that just is located installation pipe 21 on carrying the lamellar body 1, and sealing ring 24 cup joints in installation groove 14.
In this embodiment, the filter screen pipe 27 can be set to have a height of 1.5cm, and the sealing ring 24 can be clamped in the mounting groove 14 for limiting the filter screen pipe 23, so that the filter screen pipe 23 can perform a filtering function. The filter screen micropore is 8 um.
Further, two limiting grooves 16 are symmetrically formed in the mounting groove 14, the outer peripheral wall of the sealing ring 24 is fixedly connected with two limiting pieces 241 matched with the two limiting grooves 16, and the two limiting pieces 241 are respectively clamped in the two limiting grooves 16.
In the present embodiment, the sealing ring 24 is clamped in the mounting groove 14, and can also be correspondingly clamped in the two limiting grooves 16 through the two limiting pieces 241, so as to prevent the filter screen pipe 23 and the dropping pipe 22 from rotating.
Furthermore, a penetrating finger slot 15 is formed on the upper surface of the slide glass body 1 and between the two front blocking pieces 12.
In this embodiment, the finger groove 15 can provide a finger operation space for placing and taking out the special slide 3 and the slide clamping piece 10, thereby facilitating the slide making operation.
Further, the height of the front flap 12 is smaller than the height of the rear flap 13.
In the present embodiment, after the special slide 3 is placed between the two slots 111, the front stop piece 12 and the rear stop piece 13 can effectively limit the special slide 3.
Further, the lower surface of the glass clip 41 is provided with a through finger hole 44.
In this embodiment, finger holes 44 facilitate the installation or removal of glass slide clips 41 by a worker.
Further, a plurality of convex strips 43 which are equidistant and uniformly arranged are integrally formed on the lower surface of the glass carrying clamping piece 41 and located on one side of the finger hole 44, and a convex block 45 is integrally formed on the lower surface of the glass carrying clamping piece 41 and located on the other side of the finger hole 44.
In this embodiment, the friction force between the slide glass clip 41 and the fingers can be increased when the slide glass clip 41 is held by the slide maker, thereby preventing the problem of slipping when the slide glass clip 41 is mounted and removed.
A use method of an exfoliated cell immunochemical slide maker comprises the following steps:
step one, adopting a 50ml disposable pointed-bottom centrifuge tube, taking 50-100 ml of liquid at the bottom of a body cavity effusion container (1-2 centrifuge tubes per sample), centrifuging for 5 minutes at the rotating speed of 2500-3000 rpm, and discarding supernatant.
And step two, when the content of the precipitated blood is more than 1/2 of the precipitate, washing the precipitate for 5-10 minutes by 15-20ml of 10% glacial acetic acid, then centrifuging the precipitate for 5 minutes at the rotation speed of 1200 rpm, washing the precipitate by Phosphate Buffer Solution (PBS), centrifuging the precipitate for 5 minutes at the rotation speed of 1200 rpm, and centrifuging the precipitate twice. This step can be omitted when the blood content is low.
And step three, when the amount of the precipitate is greater than 2ml, sucking 1/2 of the precipitate, placing the precipitate into a liquid-based storage bottle for fixation and other treatment, and when the amount is less than 2ml, placing all the precipitate into the liquid-based storage bottle.
Step four, 3ml of the cell suspension is added into the sheet making device, and then 2ml of cell cleaning solution is added;
and step five, placing the tabletting machine in a centrifugal tabletting machine, and centrifuging for 5 minutes at the rotating speed of 1200 rpm.
And step six, taking out the slide maker from the centrifugal slide maker, dumping liquid on the upper part of the slide maker, removing the limitation of the limiting mechanism in the slide maker, and taking out the special glass slide for natural drying or drying.
In the embodiment, for the immunochemical detection of cervical fluid-based cells, firstly, cervical cells are brushed down by using a brush body and used as preservation fluid samples, a worker divides the preservation fluid samples into a plurality of equal parts according to pathological operation specifications in sequence and registers the preservation fluid samples with the labels, and the preservation fluid samples registered with the labels are shaken by hands or shakes a test tube, so that the preservation fluid samples vibrate for 12 minutes, the cells on the brush body can be favorably and fully shed, and the shed cells are fully suspended in liquid to prepare cell suspension. The body cavity effusion sample is centrifuged for the first time at 2500-3000 rpm, and the centrifugation is not less than 5 minutes (high rotation speed, short time), so that more cells in the liquid can be collected as much as possible to prepare cell suspension. After the preparation, the worker took out 3ml of the cell suspension and added it to the dropping tube of the slide maker, and then 2ml of the cell cleaning solution was added, and 3ml of the cell suspension and 2ml of the cell cleaning solution in the dropping tube were mixed. After dropwise add liquid and accomplish, the staff places the film-making ware in the centrifugation film-making machine, 1200 rpm rotational speed centrifugation 5 minutes, after the centrifugation finishes, the staff takes out the film-making ware, and relieve the limit function of the stop gear of film-making ware bottom, can take out special slide glass, then carry out natural air-drying or stoving special slide glass, just can prepare and accomplish and obtain the cell immunity chemistry smear, do not need expensive full-automatic film-making equipment, can prepare cell immunity chemistry piece fast, prepare liquid-based thin layer TCT smear simultaneously in the lump, operation method is swift simple and convenient.
The working principle and the using process of the invention are as follows: after the installation of the invention, when the special glass slide 3 is installed, the special glass slide 3 firstly moves from the front end of the glass slide body 1 with the front baffle 12 to the rear end of the glass slide body 1 with the rear baffle 13, and the special glass slide 3 is clamped between the two limit plates 11, after the special glass slide 3 is moved and installed, the two limit plates 11 clamp the glass slide, the glass slide clamping piece 41 is operated according to the same steps of the special glass slide 3, the glass slide clamping piece 41 is clamped between the two limit plates 11, and glass slide clamping piece 41 is located the lower surface of special slide glass 3 and compresses tightly special slide glass 3 in order to restrict its removal, and glass slide clamping piece 41 is located between two preceding separation blades 12 and back separation blade 13, and two preceding separation blades 12 block glass slide clamping piece 41 with back separation blade 13, and dropping pipe 22 is used for the cell preservation liquid dropwise add of treating the film-making and carries out the film-making operation on special slide glass 3, and card handle 25 is used for placing this film-making ware on centrifugal film-making machine's film-making station fixedly.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. An exfoliated cell immunochemical slide maker, comprising:
the device comprises a carrying body (1), wherein limiting plates (11) extend downwards from two ends of the carrying body (1) respectively, two front blocking pieces (12) are arranged at one end of the lower surface of the carrying body (1), and a rear blocking piece (13) is arranged at the other end of the lower surface of the carrying body (1);
the liquid dropping mechanism (2) comprises a mounting pipe (21) fixedly communicated with the upper surface of the carrier body (1), a dropping pipe (22) communicated with the mounting pipe (21) is mounted on the mounting pipe (21), and two clamping handles (25) are symmetrically and fixedly connected to the peripheral wall of the dropping pipe (22);
the special glass slide (3), the special glass slide (3) is clamped between the two limiting plates (11);
limiting mechanism (4), limiting mechanism (4) are including joint in slide glass clamping piece (41) between two limiting plates (11), slide glass clamping piece (41) are located the below of special slide glass (3) and closely laminate.
2. An exfoliated cell immunochemical slide according to claim 1, wherein: two draw-in grooves (111) have been seted up respectively on the opposite face of limiting plate (11), glass carrier clamping piece (41) both ends respectively downwardly extending have with parting bead (42) of draw-in groove (111) looks adaptation.
3. An exfoliated cell immunochemical slide as claimed in claim 2, wherein: the division bar (42) is arranged in a wedge shape.
4. An exfoliated cell immunochemical slide according to claim 1, wherein: dropping liquid mechanism (2) still establish including the cover filter tube net (23) of installation pipe (21) inner chamber, filter tube net (23) top with dropwise add pipe (22) integrated into one piece, filter tube net (23) bottom fixedly connected with sealing ring (24), just be located on carrier piece body (1) mounting groove (14) have been seted up in the outside of installation pipe (21), sealing ring (24) cup joint in mounting groove (14).
5. An exfoliated cell immunochemical slide according to claim 4, wherein: two spacing grooves (16) have been seted up to the symmetry on mounting groove (14), sealing ring (24) periphery wall fixedly connected with and two spacing piece (241) of spacing groove (16) looks adaptation, two spacing piece (241) joint respectively in two in spacing groove (16).
6. An exfoliated cell immunochemical slide according to claim 1, wherein: the upper surface of the carrier body (1) is positioned between the two front blocking pieces (12) and is provided with a through finger groove (15).
7. An exfoliated cell immunochemical slide according to claim 1, wherein: the height of the front baffle plate (12) is smaller than that of the rear baffle plate (13).
8. An exfoliated cell immunochemical slide according to claim 1, wherein: the lower surface of the glass carrying clamping piece (41) is provided with a penetrating finger hole (44).
9. An exfoliated cell immunochemical slide according to claim 8, wherein: glass carrier clamping piece (41) lower surface just is located one side integrated into one piece that indicates hole (44) has a plurality of equidistance, sand grip (43) of evenly arranging, glass carrier clamping piece (41) lower surface just is located the opposite side integrated into one piece that indicates hole (44) has lug (45).
10. A method of using a tableting machine as claimed in any of claims 1 to 9, characterised by: the method comprises the following steps:
step one, adopting a 50ml disposable pointed-bottom centrifuge tube, taking 50-100 ml of liquid at the bottom of a body cavity effusion container (1-2 centrifuge tubes per sample), centrifuging for 5 minutes at the rotating speed of 2500-3000 rpm, and discarding supernatant.
And step two, when the content of the precipitated blood is more than 1/2 of the precipitate, washing the precipitate for 5-10 minutes by 15-20ml of 10% glacial acetic acid, washing the precipitate for 5 minutes at the rotation speed of 1200 rpm, washing the precipitate for 5 minutes by using Phosphate Buffer Solution (PBS), and centrifuging the precipitate for 5 minutes at the rotation speed of 1200 rpm twice. This step can be omitted when the blood content is low.
And step three, when the amount of the precipitate is greater than 2ml, sucking 1/2 of the precipitate, placing the precipitate into a liquid-based storage bottle for fixation and other treatment, and when the amount is less than 2ml, placing all the precipitate into the liquid-based storage bottle.
Step four, 3ml of the cell suspension is added into the sheet making device, and then 2ml of cell cleaning solution is added;
and step five, placing the tabletting machine in a centrifugal tabletting machine, and centrifuging for 5 minutes at the rotating speed of 1200 rpm.
And step six, taking out the slide maker from the centrifugal slide maker, dumping liquid on the upper part of the slide maker, removing the limitation of the limiting mechanism in the slide maker, and taking out the special glass slide for natural drying or drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010996245.XA CN112113814A (en) | 2020-09-21 | 2020-09-21 | Exfoliative cell immunochemistry sheet making device and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010996245.XA CN112113814A (en) | 2020-09-21 | 2020-09-21 | Exfoliative cell immunochemistry sheet making device and using method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN112113814A true CN112113814A (en) | 2020-12-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010996245.XA Pending CN112113814A (en) | 2020-09-21 | 2020-09-21 | Exfoliative cell immunochemistry sheet making device and using method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130034874A1 (en) * | 2010-03-22 | 2013-02-07 | Novacyt | Automatic process and automated device for preparing and analysing a plurality of cell suspensions |
| CN209215037U (en) * | 2018-10-23 | 2019-08-06 | 东海县裕隆医学检验实验室有限公司 | A kind of Thinprep pap test is pressed from both sides with film-making |
| CN211453055U (en) * | 2019-11-08 | 2020-09-08 | 常丽珍 | Exfoliative cell immunohistochemical film-making device |
-
2020
- 2020-09-21 CN CN202010996245.XA patent/CN112113814A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130034874A1 (en) * | 2010-03-22 | 2013-02-07 | Novacyt | Automatic process and automated device for preparing and analysing a plurality of cell suspensions |
| CN209215037U (en) * | 2018-10-23 | 2019-08-06 | 东海县裕隆医学检验实验室有限公司 | A kind of Thinprep pap test is pressed from both sides with film-making |
| CN211453055U (en) * | 2019-11-08 | 2020-09-08 | 常丽珍 | Exfoliative cell immunohistochemical film-making device |
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Application publication date: 20201222 |