CN112111410B - Preparation method of dibenzooxepinone compound - Google Patents

Abstract

The invention relates to a preparation method of dibenzooxepinone compounds from marine fungi WH4-2, wherein the marine fungi staphyloccocus has high-efficiency anti-vibrio parahaemolyticus activity, and the yield of active substances, namely the isopentenyl-containing dibenzooxepinone compounds, is high, so that the compounds are expected to be applied to the development of novel anti-vibrio parahaemolyticus drugs.

Description

Preparation method of dibenzoxepin compound

The application is a divisional application of a Chinese patent 201810207291X dibenzoxepin compound and a preparation method and application thereof, and the application date of the original application is 2018-03-14; application No. 201810207291X; the invention creates the name: dibenzoxepin compounds, and preparation methods and uses thereof.

Technical Field

The invention relates to a preparation method of a compound, in particular to a preparation method of a secondary metabolite of marine fungi, namely isopentenyl-containing dibenzoxepin compound, and application of the secondary metabolite of the marine fungi in the aspects of vibrio parahaemolyticus (V.parahaemolyticus) resistance, bacillus subtilis resistance, methicillin-resistant staphylococcus aureus and enterococcus faecium activity.

Background

Parahemolytic vibrio is vibrio of vibrionaceae, and is rod-shaped, filamentous or slightly bent, gram-negative bacterium, halophilic. The distribution is extremely wide, but the pathogenic bacteria are mainly distributed in seawater and marine products, are the most common pathogenic bacteria in mariculture, and once outbreak occurs, the process is fast and is not easy to control. Antibiotic drugs are the main means of prevention and treatment at present, but a large amount of long-term use pollutes the marine water environment, and simultaneously causes the continuous generation of drug-resistant strains. Food containing vibrio parahaemolyticus is taken by people to cause food poisoning, severe patients can cause shock due to dehydration, and a few patients can die. In the aspect of treatment, quinolone antibacterial drugs can be used for patients with serious illness, but the drug resistance of bacteria is easily caused by the large use of quinolone antibiotics. The development of new, highly effective, low-toxic and environmentally friendly vibrio parahemolyticus drugs is imminent.

In earlier work, a marine fungus WH4-2 with antibacterial activity on multiple pathogenic bacteria is separated and screened from bottom sediment of a sea area near the Weihai, and the marine fungus is identified as the panus armeniaca through ITS complete sequence analysis. The secondary metabolite research of Caliper currently reports only 4. Lynn DG et al, from Caliper species, obtained spiroketal structures that block the outflow of potassium ions, leading to muscle dysfunctionPolyether compounds of (1) [ Journal of the American Chemical Society,1982,104(25):7319-7322]. O' Sullivan et al isolated and identified Paecilomyces petiolatus and studied its biosynthetic pathway [ Bioorganic Chemistry,1995,23(2),131-]. Zang Y et al found polyketides from Talaromyces, one of which had significant antimalarial activity against chloroquine-tolerant Plasmodium [ Phytochemistry 2015,119,70-75 [ ]]. Zang Y et al also isolated polyketides that significantly inhibited the growth of gram-positive bacteria and Hela tumor cells [ Journal of Natural Products,2016,79(12):2991-]. The crude extract of the fermentation broth of the fungus Talaromyces subthaeformis has never been found in the previous literature and patents to be resistant to Vibrio parahaemolyticus. The subject group finds that the isopentenyl-containing dibenzoxepin compound is an antibacterial active ingredient of the compound through further analysis, and the compound is obtained from the pannaga fungi for the first time. Isopentenyl-containing dibenzoxepin compounds Only 17 Natural Products were found so far, all isolated from fungi of different origins [ Journal of Natural Products,2006,69(7), 995-1000; organic Letters,2013,15(8), 2058-2061; molecules,2016,21,1184-1195]. Few researches on the biological activity of the isopentenyl-containing dibenzoxepin compound are reported. Dibenzoxepin Arugosins H has anti-chlorella activity. In the antibacterial aspect, dibenzoxepin Arugosins A, B and H have certain antibacterial activity; antitumor dibenzoxepin Arugosins A and B mixtures show moderate inhibitory activity against multiple tumor cells at a concentration of 10. mu.g/mL [ Journal of Natural Products,2006,69(7),995 1000-]. The isopentenyl dibenzoxepin 5R-Arugosine K, 5S-Arugosine K, Arugosine N and CAS:160585-91-1 compounds of this patent, which contain antibacterial active substances, were isolated in 2016 from another of a different species from the same genus, but no biological activity analysis was carried out [ Molecules,2016,21,1184-]. The compound "Arugosin K" appears under the same name in another document [ Journal of Antibiotics,2017,70(2),174-]However, the structure is not the dibenzoxepin Arugosin K isolated from us, and the invention adopts the corresponding name of the structure in this paper in the published orderCalled [ Molecules,2016,21,1184-]. Japanese patent application No. JP06271561 discloses that a compound isolated from Penicillium fungi (CAS:160585-91-1) has the activity of inhibiting leukemia cell HL-60 (IC)₅₀At 5 μ g/mL) but did not involve any antimicrobial activity studies.

Disclosure of Invention

The purpose is as follows: the invention provides a preparation method of dibenzooxepin compounds derived from marine fungi.

The technical scheme is as follows: in order to solve the technical problems, the technical scheme adopted by the invention is as follows:

the invention discovers for the first time that the ocean fungi socallella sessilifolia has high-efficiency vibrio parahaemolyticus resistance activity, discovers for the first time that a secondary metabolite 'isopentenyl-containing dibenzooxepinone compound' has remarkable vibrio parahaemolyticus resistance activity, and shows certain antibacterial activity on Bacillus subtilis, methicillin-resistant Staphylococcus aureus (Staphylococcus aureus ATCC29213) and Enterococcus faecium (Enterococcus faecium ATCC 35667).

The invention aims to obtain marine fungi with remarkable vibrio parahaemolyticus resistance activity, and isolate and identify isopentenyl-containing dibenzooxepin compounds with high-efficiency vibrio parahaemolyticus inhibition activity from the marine fungi, and further antibacterial experimental analysis shows that the compounds also have certain inhibition activity on bacillus subtilis, methicillin-resistant staphylococcus aureus and enterococcus faecium.

According to the invention, marine fungi are separated from offshore sediment collected from a sea area near the Weihai in Shandong (the sampling time is 2015 year and 10 months), a filter paper method is adopted to screen the vibrio parahaemolyticus inhibition activity of a separated strain fermentation product, a strain WH4-2 with the vibrio parahaemolyticus inhibition activity is found, and the primary screening of the filter paper method shows that a crude extract of a fermentation liquid of the strain has an obvious inhibition effect on test bacteria at 150 mu g/piece. The ITS complete sequence analysis (sequencing analysis by Shanghai Biotechnology engineering service Co., Ltd.) finds that the similarity of the strain WH4-2 and the Talaromyces stipitatus reaches 98%, and the strain WH4-2 is identified as the Talaromyces by integrating the colony morphology and the structural characteristics of the compound obtained by later separation. GenBank sequence number is MG 877637. The strain is fermented in a large scale, and Fr.A eluted from the extract by silica gel reduced pressure column chromatography and dichloromethane has obvious inhibition effect on vibrio parahaemolyticus when the Fr.A is 150 mu g/tablet. Fr.a is chromatographed on a silica gel atmospheric pressure column with a petroleum ether/ethyl acetate gradient, where petroleum ether: ethyl acetate 30:1 from fr.a-1 and petroleum ether: Fr.A-5 eluted from ethyl acetate 3:1 is an anti-vibrio parahaemolyticus active component. Fr.a-1 was purified by semi-preparative HPLC with mobile phase methanol: water 90:10 gave compound 1(TA-4,100.0 mg). Fr.a-5 was purified by semi-preparative HPLC, methanol: 80 portions of water, and 20 portions of compound 2(TA-7,100.0 mg). Compound 1 and compound 2 are both isovalerenyl-containing dibenzoxepin compounds, compound 1 is a mixture of the 5R-Arugosine K and 5S-Arugosine K enantiomers, and compound 2 is a mixture of Arugosine N and its isomer (CAS: 160585-91-1). The MIC values of the minimum inhibitory concentrations of Compound 1 and Compound 2 were found to be 12.5 and 3.13. mu.g/mL, respectively, using the microdilution method. Further antibacterial experiment analysis shows that the compound 2 has certain inhibitory activity on bacillus subtilis, methicillin-resistant staphylococcus aureus and enterococcus faecium, and the minimum inhibitory concentration MIC values are 3.13,12.5 and 12.5 mu g/mL respectively.

The stipes lobata WH4-2(Talaromyces stipitatus) is preserved in China general microbiological culture Collection center in 2018, 1 month and 31 days, the preservation address is No. 3 of Xilu 1 of Beijing Kogyo sunward, and the strain preservation number is CGMCC NO. 15280.

Has the advantages that: the marine fungi obtained by separation has high-efficiency vibrio parahaemolyticus resisting activity, and the minimum inhibitory concentration MIC values of the prepared dibenzoxepin compound 1 and the compound 2 are 12.5 mu g/mL and 3.13 mu g/mL respectively. The strain has high yield of active ingredients (5.0mg/L), and is expected to be applied to the development of medicaments for resisting vibrio parahaemolyticus. The compound 2 has certain inhibitory activity on bacillus subtilis, methicillin-resistant staphylococcus aureus and enterococcus faecium, the minimum inhibitory concentration MIC values are 3.13,12.5 and 12.5 mu g/mL respectively, and the compound is expected to be applied to development of broad-spectrum antibacterial drugs.

Drawings

FIG. 1 is a characteristic colony plot of strain WH 4-2;

FIG. 2 is a drawing of Compound 1¹H NMR spectrum;

FIG. 3 is an APT spectrum of Compound 1;

FIG. 4 is an HSQC spectrum of Compound 1;

FIG. 5 is an HMBC spectrum of compound 1;

FIG. 6 is a HR-ESI-MS spectrum of Compound 1;

FIG. 7 is a drawing of Compound 2¹H NMR spectrum;

FIG. 8 is a HR-ESI-MS spectrum of Compound 2.

Detailed Description

To further illustrate the present invention, a series of examples are given below, which are purely illustrative and are intended to be a detailed description of the invention only and should not be understood as limiting the invention.

Collecting samples: samples of sediment were collected offshore in the sea area near the east west sea in 2015 for 10 months and stored in sterile bottles.

1 isolation and culture of Strain WH4-2

The coating dilution method is adopted. Taking 10mL of sediment sample, placing the sediment sample in a small 50mL beaker, adding 10mL of sterile seawater, fully mixing the mixture, and standing the mixture for 10 minutes. A10-fold dilution of 1mL of the supernatant in sterile water was used as a gradient control. 0.2mL each of the supernatant liquid and 10-fold diluted liquid was taken by a pipette and added to PDA (potato 200g/L, glucose 20g/L, sea salt 30g/L and agar 18g/L) and Bengal red plate containing antibiotics, and they were cross-coated with a coater. Each concentration gradient of each medium was performed in 3 replicates. Sealing with sealing film, and culturing in a fungus culture box at 28 deg.C by inversion for 2-3 w. Observing the growth condition of the fungi from the 3 rd day of culture, observing for 3 times every day, observing once in the morning, at noon and evening, finding out new bacterial colony growing out, inoculating the bacterial colony onto another new plate by using a sterile bamboo stick, culturing for 4-5 days in an inverted incubator at 28 ℃, and repeating the processes until a single bacterial colony with single bacterial colony characteristic is obtained. A1000 mL conical flask was filled with 500mL of PDB (agar was not added to PDA formulation) liquid medium, sterilized at high temperature, and the separated strain WH4-2 and the other 22 strains were picked up from the plate with tweezers and inoculated into PDB medium (2 flasks were inoculated per strain) respectively, and then cultured at 28 ℃ for 40 days to obtain 1L of fermentation broth. Extracting with ethyl acetate solution containing 5% acetone twice, collecting ethyl acetate, and concentrating under reduced pressure to obtain crude extract 375 mg.

2 antibacterial Activity screening (Filter paper method)

Pouring sterilized LB solid culture medium (5 g of yeast extract, 10.0g of tryptone, 10.0g of NaCl, 1000mL of pure water and 18g of agar powder added into the solid culture medium) into a culture dish, uniformly coating overnight liquid-cultured vibrio parahaemolyticus on the solid culture medium, preparing a solution with the concentration of 50mg/mL from a crude extract by using methanol, sucking 3 mu L (namely 150 mu g/piece) of the solution, dropwise adding the solution onto a 6 mm-diameter filter paper piece, volatilizing the solvent, attaching a medicine-adding face to the culture medium, enabling each sample to be parallel, sealing the culture dish attached with the filter paper piece, placing the culture dish in a 28 ℃ constant-temperature culture box for inverted culture for 18-24 hours, and measuring the diameter of a bacteriostatic circle to be 15 mm.

Identification of 3 Strain WH4-2

On the PDA solid culture medium, the front surface of the colony is yellow white, the mycelium is short, the edge is ring-shaped, and the back surface of the colony is orange yellow (see figure 1). The ITS complete sequence analysis of the strain is determined (sequencing analysis is carried out by Shanghai biological engineering technical service Co., Ltd.), BLAST retrieval is carried out on the sequence, MEGA 5 software is applied, a phylogenetic tree is analyzed and constructed, and 1000 bootstraps tests are carried out, so that the similarity of the strain WH4-2 and the pannaga is found to reach 98%. In combination with the structural types of the compounds identified by the following separation, strain WH4-2 was identified as Caulerpa, GenBank accession number MG 877637.

4 bacterial strain large-scale fermentation

Taking out the strain from an ultralow temperature refrigerator at minus 80 ℃, standing to room temperature, clamping a strain block containing hyphae, sticking the strain block on a PDA culture medium, and performing inverted culture at 28 ℃ for 3-5 days to obtain a pure colony. And (3) filling 100mL of PDB liquid culture medium into a 250mL conical flask, sterilizing at high temperature of 120 ℃, inoculating a proper amount of recovered bacterial colonies, and performing shaking culture at the temperature of 28 ℃ and 120r/min for 3d by using a shaking table to obtain a seed solution. And (3) filling 400mL of PDB liquid culture medium into a 1000mL conical flask, sterilizing at high temperature, adding the seed solution according to the inoculation amount of 1:40, inoculating 100 bottles, and standing and culturing for 40 days at 28 ℃ to obtain about 40 liters of fermentation liquor.

5 Activity directed separation

Filtering the fermentation liquid, collecting 40L culture solution, extracting with ethyl acetate solution containing 5% acetone for 2 times, collecting ethyl acetate, concentrating under reduced pressure to obtain total extract 18g, and performing antibacterial activity analysis by using filter paper sheet method, wherein the diameter of the antibacterial zone is 15mm (150 μ g/sheet). Subjecting the extract to silica gel reduced pressure column chromatography, and gradient eluting with chloroform/methanol system to obtain 12 components, wherein Fr.A (1.5g) eluted from dichloromethane has strong inhibitory activity, and the diameter of inhibition zone is 19mm (150 μ g/tablet). From.a, silica gel atmospheric pressure column chromatography, eluting with petroleum ether/ethyl acetate gradient, yielded 5 components, where petroleum ether: ethyl acetate 30:1 from fr.a-1(400mg) and petroleum ether: ethyl acetate 3:1 elution fr.a-5(200mg) was the antibacterial active ingredient, with the fr.a-1 and fr.a-5 zone diameters of 10 and 25mm (150 μ g/tablet), respectively. Fr.a-1 was purified by semi-preparative HPLC with mobile phase methanol: water 90:10 gave compound 1(TA-4,100.0 mg). Fr.a-5 was purified by semi-preparative HPLC, methanol: 80 portions of water, and 20 portions of compound 2(TA-7,100.0 mg).

2.6 structural characterization of Compound 1 and Compound 2

Comprehensive utilization of multiple spectroscopic methods (nuclear magnetic resonance spectroscopy, hydrogen spectroscopy, etc.)¹H NMR, proton coupling test (APT), and correlation spectrum of hydrogen homonuclear chemical shift (¹H-¹H COSY), heteronuclear single quantum correlation spectrum (HSQC), heteronuclear multiple bond correlation spectrum (HMBC), high resolution mass spectrum (HRESIMS)]The combination literature comparison confirms that the compound 1 and the compound 2 are both isopentenyl-containing dibenzoxepin compounds. Wherein Compound 1 (5R-Arugosine K and 5S-Arugosine K) is a mixture of a pair of enantiomers. Compound 2(Arugosin N and CAS:160585-91-1) is a mixture of a pair of tautomers which cannot be separated by HPLC, and the interconversion is due to the presence of an unstable hemiacetal (C-5) fragment in both structures, the hemiacetal ring opening, the phenyl ring (ring A) connecting the isopentenyl groupsCan rotate through a C11-C12 bond, and when the positions of two hydroxyl groups (C-6 and C-10) at meta positions on the A ring are exchanged, the rotated hydroxyl groups can perform aldol condensation with the C-5 position to form isomers (Molecules,2016,21, 1184-1195). The spectral data for compound 1 and compound 2 are as follows:

5R(S)Arugosin K,

0°(c 0.5,CDCl₃)，HRESI-MS m/z：377.1376[M+Na]⁺(calc.377.1359)。¹H NMR(600MHz,CDCl₃):δ_H 13.63(1H,s,10-OH),11.46(1H,s,14-OH),7.34(1H,d,J＝8.2Hz,H-8),6.94(1H,s,H-3),6.87(1H,s,H-15),6.57(1H,d,J＝8.2Hz,H-7),5.65(1H,s,H-5),5.24(1H,brt,J＝7.2Hz,H-2’),3.57(3H,s,5-OCH₃),3.33(2H,d,J＝7.2Hz,H-1’),2.38(3H,s,H-1),1.77(3H,s,H-4’),1.73(3H,s,H-5’)。¹³C NMR(100MHz,CDCl₃):δ_C 197.6(C-12,C),162.9(C-10,C),162.5(C-14,C),154.7(C-6,C),147.3(C-2,C),138.6(C-4,C),137.8(C-8,CH),133.4(C-3’,C),125.0(C-9,C),121.9(C-2’,CH),119.7(C-15,CH),117.0(C-3,CH),117.0(C-13,C),113.8(C-9,C),109.4(C-7,CH),103.5(C-5,CH),57.1(5-OCH₃),28.0(C-1’,CH₂),26.0(C-4’,CH₃),22.0(C-1,CH₃),17.9(C-5’,CH₃)。

CAS:160585-91-1,HRESI-MS m/z：363.1206[M+Na]⁺(calc.363.1203)。¹H NMR(600MHz,CDCl₃):δ_H 13.61(1H,s,10-OH),11.54(1H,s,14-OH),7.32(1H,d,J＝8.2Hz,H-8),7.04(1H,s,H-3),6.89(1H,s,H-15),6.87(1H,s,H-15),6.53(1H,d,J＝8.2Hz,H-7),6.12(1H,s,H-5),5.31(1H,brt,J＝7.2Hz,H-2’),3.33(2H,d,J＝7.2Hz,H-1’),2.40(3H,s,H-1),1.76(3H,s,H-4’),1.72(3H,s,H-5’)。

Arugosin N,HRESI-MS m/z：363.1206[M+Na]⁺(calc.363.1203)。¹H NMR(600MHz,CDCl₃):δ_H 12.95(1H,s,6-OH),11.82(1H,s,14-OH),7.34(1H,d,J＝8.2Hz,H-8),7.07(1H,s,H-3),6.89(1H,s,H-15),6.71(1H,d,J＝8.2Hz,H-7),6.11(1H,s,H-5),5.24(1H,brt,J＝7.2Hz,H-2’),3.36(1H,m,H-1’a),3.32(1H,m,H-1’b),2.40(3H,s,H-1),1.76(3H,s,H-4’),1.74(3H,s,H-5’)。

2.7 antibacterial Activity test (microdilution method) for Compound 1 and Compound 2

Diluting the prepared bacterial seed culture solution to 1:800 of the original concentration. And (3) taking a 96-hole micro-dilution plate, adding 198 mu L of bacterial liquid into the first row of the plate, then adding 2 mu L of sample, adding 100 mu L of bacterial liquid into the other holes, sucking the uniformly mixed sample and bacterial liquid in the 100 mu L first row, sequentially adding the uniformly mixed sample and bacterial liquid downwards, diluting by twice decreasing concentration, finally filling 200 mu L of each hole, and setting three parallel concentrations. And setting ampicillin sodium positive control, a culture medium blank control group and DMSO negative control, culturing at 28 ℃ for 18-24h, and measuring absorbance at 600nm by using an enzyme-labeling instrument to obtain the inhibition rate and the Minimum Inhibitory Concentration (MIC).

In-vitro antibacterial experiments show that the compound 1 and the compound 2 have obvious inhibitory activity on vibrio parahaemolyticus, and MIC values are 12.5 and 3.13 mu g/mL respectively. The compound 2 is found to show certain inhibitory activity to bacillus subtilis, methicillin-resistant staphylococcus aureus and enterococcus faecium, and the minimum inhibitory concentration MIC values are 3.13,12.5 and 12.5 mu g/mL respectively.

Example 1

Synthesis of 2- (1-hydroxy-n-pentyl) benzoic acid (III):

1.24g (6.5mmol) of NBP was dissolved in 10mL of methanol, 10mL of 2M NaOH solution was added, the mixture was refluxed and stirred for 0.5h, the methanol was distilled off under reduced pressure, 10mL of distilled water was added for dilution, the mixture was cooled to-5 ℃ and acidified to pH 2-3 with 5% dilute hydrochloric acid under vigorous stirring, extracted with ether (15 mL. times.3) and directly put into the next reaction without any purification.

Example 2

Synthesis of 2- (1-acetyl-n-pentyl) benzoic acid:

the above ether solution containing III was diluted with 200mL of dichloromethane, 2.7mL (19.6mmol) of triethylamine and 0.5g of DMAP were added dropwise at-10 ℃ to 1.4mL (19.6mmol) of acetyl chloride, and after completion of dropwise addition, the mixture was stirred at-10 ℃ for 5 hours, 10mL of water was added,stirring at room temperature for 0.5h, separating organic layer, Na₂SO₄Drying, filtering, concentrating to obtain a waxy solid, and recrystallizing with n-hexane to obtain white needle crystal 1.06g with yield of 65%. mp 65-66 deg.C, MS (ESI) M/z 249.1[ M-H ]]-.¹H NMR(300MHz,CDCl₃):δ0.93(t,3H,CH₃,J＝8.5Hz),1.37–1.42(m,4H,2×CH₂),1.88–1.91(m,2H,CH₂),2.13–2.33(m,3H,COCH₃),6.61–6.72(m,1H,OCHCH₂),7.37–7.40(m,1H,ArH),7.56–7.62(m,2H,ArH),8.05(d,1H,ArH,J＝8.1Hz),10.98(brs,1H,COOH).¹³C NMR(75MHz,CDCl₃):δ172.0,166.5,140.8,133.1,130.3,130.0,127.1,125.7,74.8,41.0,36.3,27.8,22.4,13.8。

Example 3

Coupling of diols on the basis of 2- (1-acetyl-n-pentyl) benzoic acid A representative intermediate V₁The synthesis of (2):

2- (1-acetyl-n-pentyl) benzoic acid (2.50g,10.0mmol) was dissolved in anhydrous dichloromethane (50mL), EDAC (2.29g,12.0mmol) and a catalytic amount of DMAP were added, the mixture was stirred at room temperature for 0.5h, ethylene glycol (0.62g,10.0mmol) was added, the mixture was stirred at room temperature for 5h, filtered, concentrated under reduced pressure, and subjected to column chromatography [ petroleum ether: ethyl acetate (v: v) ═ 30: 1: (v: v) ]]1.71g of oil was obtained in 58% yield. MS (ESI) M/z 317.1[ M + Na ]]⁺.1H NMR(300MHz,CDCl₃):δ0.807(t,3H,CH₃,J＝7.0Hz),1.181-1.356(m,4H,2×CH₂),1.730-1.777(m,2H,CH₂),1.965(s,3H,COCH₃),3.823-3.862(m,2H,CH₂),4.269-4.474(m,2H,CH₂),5.206(s,1H,OH),6.452(t,1H,COOCH,J＝6.7Hz),7.197-7.265(m,1H,ArH),7.441-7.444(m,2H,ArH),7.750-7.777(m,1H,ArH).¹³C NMR(75MHz,CDCl₃):δ170.90,167.51,142.37,132.15,129.94,129.34,127.39,126.46,72.79,67.05,60.88,36.32,27.90,22.42,21.18,13.92。

Example 4

Representative intermediate V for coupling diamines on the basis of 2- (1-acetyl-n-pentyl) benzoic acid₃The synthesis of (2):

2- (1-acetyl-n-pentyl) benzoic acid (2.50g,10.0mmol) was dissolved in anhydrous dichloromethane (50mL), EDAC (2.29g,12.0mmol) and a catalytic amount of DMAP were added, the mixture was stirred at room temperature for 0.5h, octanediamine (1.44g,10.0mmol) was added, the mixture was stirred at room temperature for 8h, filtered, concentrated under reduced pressure, and subjected to column chromatography [ dichloromethane: methanol (v: v) ═ 10:1] to give 1.92g of an oil, yield 51%.

Example 5

A representative intermediate VI of Telmisartan coupled diamine₃Synthesis of (2)

Dissolving telmisartan (2.57g,5.0mmol) in anhydrous dichloromethane (200mL), adding EDAC (1.15g,6.0mmol) and a catalytic amount of DMAP, stirring at room temperature for 0.5h, adding octanediamine (1.44g,10.0mmol), stirring at room temperature for 5h, filtering, concentrating under reduced pressure, and performing column chromatography [ dichloromethane: methanol (v: v) ═ 30: 1%]1.48g of a white solid was obtained with a yield of 46%. mp 113 deg.C, MS (ESI) M/z641.4[ M + H [)]⁺.1H NMR(300MHz,CDCl₃):δ0.799(t,3H,CH₃,J＝6.8Hz),0.943-1.058(m,8H,4×CH₂),1.506(m,4H,2×CH₂),1.728-1.798(m,2H,CH₂),2.374(s,3H,CH₃),2.731(t,2H,NH₂CH₂,J＝7.2Hz),2.839(t,2H,CH₂,J＝7.7Hz),3.137(t,2H,NHCH₂,J＝6.1Hz),3.728(s,3H,NCH₃),5.398(s,2H,NCH₂),7.015(s,1H,ArH),7.041(s,1H,ArH),7.204(m,4H,ArH),7.251-7.312(m,6H,ArH),7.405(m,1H,NH),7.468-7.491(m,1H,ArH),7.670-7.698(m,1H,ArH).¹³C NMR(75MHz,CDCl₃):169.59,159.58,154.56,143.08,142.47,140.04,138.58,136.45,136.05,135.33,135.08,130.12,129.99,129.44,129.31,128.42,127.69,126.40,123.69,122.71,122.52,119.29,109.63,109.02,56.14,43.98,39.66,35.15,31.90,31.81,29.70,29.33,26.41,26.36,22.66,16.94,14.08。

Example 6

2-O- {2- [ (1-acetoxy) n-pentyl]Benzoyl telmisartan ethylene glycol ester (I)₁) And (4) synthesizing.

Reacting the intermediate V₁(1.47g,5.0mmol) was dissolved in anhydrous dichloromethane (30mL), EDAC (1.15g,6.0mmol) and a catalytic amount of DMAP were added, stirred at room temperature for 0.5h, telmisartan (2.57g,5.0mmol) was added, stirred at room temperature for 8h, filtered, concentrated under reduced pressure, and subjected to column chromatography [ dichloromethane: methanol (v: v) ═ 50:1]1.69g of a white solid was obtained in a yield of 43%. MP is 82-83 ℃ and MS(ESI):m/z 791.4[M+H]+,813.4[M+Na]+.¹H NMR(300MHz,CDCl₃):δ0.772(m,3H,CH₃),0.989(m,3H,CH₃),1.181(m,4H,2×CH₂),1.712(m,2H,CH₂),1.808(m,2H,CH₂),1.973(s,3H,ArCH₃),2.691(s,3H,COCH₃),2.871(m,2H,NCNCH₂),3.716(s,3H,NCH₃),3.973-4.310(m,4H,2×OCH₂),5.369(s,2H,NCH₂),6.478(m,1H,OCH),6.948(s,1H,ArH),7.023(s,1H,ArH),7.120-7.302(m,7H,ArH),7.323-7.423(m,5H,ArH),7.722-7.796(m,3H,ArH).¹³C NMR(75MHz,CDCl₃):δ169.19,167.27,165.83,156.09,154.24,143.63,142.67,142.37,141.70,140.76,136.14,134.49,132.12,131.09,130.26,130.01,129.73,129.58,128.97,128.49,126.94,126.65,125.60,125.32,123.44,121.97,121.80,119.02,109.04,108.53,72.35,62.25,61.78,46.50,36.10,31.29,29.29,27.57,21.98,21.45,20.68,16.41,13.59,13.50。

Example 7

4-O- {2- [ (1-acetoxy) n-pentyl]Benzoyl-butanediol telmisartan ester (I)₂) Synthesis of (2)

Referring to the procedure of example 5, the intermediate (1.61g,5.0mmol) and telmisartan (2.57g,5.0mmol) were reacted by column chromatography to give 1.84g of a white solid in 45% yield. mp 83 deg.C, MS (ESI) M/z 819.5[ M + H]+，841.4[M+Na]+.¹H NMR(300MHz,CDCl₃):δ0.779(t,2H,CH₃,J＝6.9Hz),0.855(t,1H,CH₃,J＝7.1Hz),0.963(t,3H,CH₃,J＝7.3Hz),1.160-1.304(m,4H,2×CH₂),1.495(m,2H,CH₂),1.725-1.826(m,4H,2×CH₂),1.952(s,3H,ArCH₃),2.676(s,3H,COCH₃),2.847(t,2H,NCNCH₂,J＝7.8Hz),3.671(s,3H,NCH₃),3.926-4.078(m,4H,2×OCH₂),5.339(s,2H,NCH₂),6.438(q,1H,OCH,J＝4.9Hz),7.011(m,2H,ArH),7.149-7.413(m,14H,ArH),7.695-7.741(m,3H,ArH).¹³C NMR(75MHz,CDCl₃):δ156.49,143.71,141.73,141.29,136.64,134.99,134.87,132.30,131.36,131.25,130.76,130.63,130.13,129.93,129.85,129.46,129.07,127.41,127.11,126.15,126.02,125.83,123.90,122.51,122.33,119.49,109.55,108.98,72.86,64.44,64.38,47.04,36.60,31.80,29.81,28.08,25.19,25.08,22.49,21.87,21.18,16.91,14.10,14.01。

Example 8

8-N- {2- [ (1-acetoxy) N-pentyl group]Benzoyl octanediamine telmisartan amide (I)₃) Synthesis of (2)

Referring to the procedure of example 5, the intermediate (1.88g,5.0mmol) and telmisartan (2.57g,5.0mmol) were reacted by column chromatography to give 1.78g of white solid in 41% yield. mp 94-95 deg.C, MS (ESI) M/z 873.6[ M + H ]]+，895.6[M+Na]+.¹H NMR(300MHz,CDCl₃):δ0.723(t,3H,CH₃,J＝7.2Hz),0.917(t,3H,CH₃,J＝7.3Hz),1.130(m,8H,4×CH₂),1.157(m,4H,2×CH₂),1.212(m,2H,CH₂),1.414-1.459(m,2H,CH₂),1.670-1.775(m,4H,2×CH₂),1.912(s,3H,ArCH₃),2.636(s,3H,COCH₃),2.786(t,2H,NCNCH₂,J＝7.8Hz),2.956(q,2H,NHCH₂,J＝6.6Hz),3.266(q,2H,NHCH₂,J＝6.8Hz),3.666(s,3H,NCH₃),5.302(s,2H,NCH₂),5.710(q,1H,OCH,J＝5.7Hz),6.954(s,1H,ArH),6.981(s,1H,ArH),7.134-7.157(m,5H,ArH),7.210-7.263(m,9H,ArH),7.289(m,1H,NH),7.340(m,1H,NH),7.433-7.458(m,1H,ArH),7.603-7.632(m,1H,ArH).¹³C NMR(75MHz,CDCl₃):δ171.16,169.02,168.61,155.93,154.05,142.60,142.16,139.55,138.00,137.87,136.06,135.62,135.58,134.72,134.53,129.56,129.49,128.93,128.82,127.97,127.40,127.20,127.16,125.85,125.25,123.33,122.10,121.89,118.88,109.09,108.48,73.78,46.49,39.40,39.26,36.26,31.42,31.33,30.93,29.68,29.29,29.19,28.86,28.59,28.51,27.17,26.39,26.12,21.82,21.33,20.72,16.44,13.60,13.38。

Example 9

5-O- {2- [ (1-acetoxy) n-pentyl]Benzoyl telmisartan pentadiol ester (I)₄) Synthesis of (2)

Referring to the procedure of example 5, the intermediate (1.68g,5.0mmol) and telmisartan (2.57g,5.0mmol) were reacted by column chromatography to give 1.98g of white solid in 48% yield. mp 78-79 deg.C, MS (ESI) M/z 833.5[ M + H ]]+,855.5[M+Na]+.¹H NMR(300MHz,CDCl₃):δ0.786(t,3H,CH₃,J＝6.9Hz),0.964(t,3H,CH₃,J＝7.4Hz),1.223-1.256(m,6H,3×CH₂),1.345-1.393(m,2H,CH₂),1.571(t,2H,CH₂,J＝7.4Hz),1.707-1.802(m,4H,2×CH₂),1.955(s,3H,ArCH),32.681(s,3H,COCH₃),2.849(t,2H,NCNCH₂,J＝7.8Hz),3.686(s,3H,NCH₃),3.958(t,2H,OCH₂,J＝6.5Hz),4.132(t,2H,OCH₂,J＝6.6Hz),5.356(s,2H,NCH₂),6.448(q,1H,OCH,J＝4.7Hz),6.995(s,1H,ArH),7.022(s,1H,ArH),7.140-7.205(m,6H,ArH),7.240-7.314(m,2H,ArH),7.349-7.416(m,5H,ArH),7.696-7.754(m,3H,ArH).¹³C NMR(75MHz,CDCl₃):δ169.82,167.74,166.41,155.98,154.16,143.15,142.63,142.31,141.25,140.78,136.13,134.50,134.28,131.74,130.82,130.27,129.65,129.40,128.95,128.57,126.90,126.59,125.64,125.47,125.33,123.37,123.33,122.03,121.85,119.00,109.05,108.49,72.38,64.34,64.21,46.56,36.12,31.32,29.32,27.68,27.58,27.48,22.00,21.93,21.37,20.69,16.42,13.61,13.52。

Example 10

6-O- {2- [ (1-acetoxy) n-pentyl]Benzoyl telmisartan hexanediol (I)₅) Synthesis of (2)

Referring to the procedure of example 5, the intermediate (1.75g,5.0mmol) and telmisartan (2.57g,5.0mmol) were reacted by column chromatography to give 1.82g of white solid in 43% yield. mp is 82-83 deg.C, MS (ESI) M/z 847.5[ M + H ]]+，869.5[M+Na]+.¹H NMR(300MHz,CDCl₃):δ0.796(m,3H,CH₃),0.973(t,3H,CH₃,J＝7.2Hz),1.176-1.256(m,10H,5×CH₂),1.600(m,2H,CH₂),1.757-1.806(m,4H,2×CH₂),1.966(s,3H,ArCH₃),2.687(s,3H,COCH₃),2.849(t,2H,NCNCH₂,J＝7.7Hz),3.702(s,3H,NCH₃),3.939-3.961(m,2H,OCH₂),4.169(m,2H,OCH₂),5.349(s,2H,NCH₂),6.443(q,1H,OCH),7.001-7.026(m,2H,ArH),7.154-7.203(m,8H,ArH),7.302-7.405(m,5H,ArH),7.720-7.775(m,3H,ArH).¹³C NMR(75MHz,CDCl₃):δ169.81,167.77,166.47,155.97,154.15,143.08,142.64,142.30,141.25,140.80,136.13,134.52,134.22,131.68,130.79,130.35,130.25,129.66,129.38,128.96,128.58,126.90,126.59,125.61,125.42,123.35,122.04,121.86,119.01,109.04,108.49,72.42,64.55,64.37,46.56,36.14,31.34,29.24,28.03,27.73,27.59,25.12,25.05,22.01,21.37,20.69,16.42,13.61,13.52。

Example 11

8-O- {2- [ (1-acetoxy) n-pentyl]Benzoyl-octanediol telmisartan ester (I)₆) Synthesis of (2)

Referring to the procedure of example 5, the intermediate (1.89g,5.0mmol) and telmisartan (2.57g,5.0mmol) were reacted by column chromatography to give 1.96g of white solid in 45% yield. mp 83-84 deg.C, MS (ESI) M/z 875.6[ M + H ]]+,897.5[M+Na]+.¹H NMR(300MHz,CDCl₃):δ0.806(t,3H,CH₃,J＝6.8Hz),0.981(t,3H,CH₃,J＝7.3Hz),1.179-1.237(m,8H,4×CH₂),1.452-1.474(m,6H,3×CH₂),1.627-1.695(m,2H,CH₂),1.722-1.843(m,4H,2×CH₂),1.980(s,3H,ArCH₃),2.692(s,3H,COCH₃),2.861(t,2H,NCNCH₂,J＝7.8Hz),3.712(s,3H,NCH₃),3.931(t,2H,OCH₂,J＝6.4Hz),4.204(t,2H,OCH₂,J＝6.6Hz),5.364(s,2H,NCH₂),6.455(q,1H,OCH,J＝4.6Hz),7.001-7.027(m,2H,ArH),7.158-7.286(m,8H,ArH),7.337-7.418(m,5H,ArH),7.703-7.797(m,3H,ArH).¹³C NMR(75MHz,CDCl₃):δ169.81,167.79,166.55,155.97,154.15,143.12,142.60,141.24,140.81,136.11,134.51,134.16,131.63,130.77,130.40,130.24,129.69,129.37,128.95,128.59,126.89,126.58,125.59,125.38,123.39,122.04,121.86,119.02,109.02,108.50,72.47,64.75,64.54,62.40,46.59,36.15,32.24,31.33,29.33,28.85,28.63,28.55,25.45,25.17,22.02,21.37,20.70,16.42,13.61,13.52。

Example 12

6-N- {2- [ (1-acetoxy) N-pentyl]Benzoyl-hexamethylenediamine telmisartan amide (I)₇) Synthesis of (2)

Referring to the procedure of example 5, the intermediate (1.74g,5.0mmol) and telmisartan (2.57g,5.0mmol) were reacted by column chromatography to give 1.69g of white solid in 40% yield. mp is 96-98 deg.C, MS (ESI) M/z 845.6[ M + H ]]+，867.5[M+Na]+.¹H NMR(300MHz,CDCl₃):δ0.768(m,3H,CH₃),0.970(t,3H,CH₃,J＝7.2Hz),1.181(m,10H,5×CH₂),1.417(m,2H,CH₂),1.776-1.801(m,4H,2×CH₂),1.936(s,3H,ArCH₃),2.645(s,3H,COCH₃),2.843(t,2H,NCNCH₂,J＝7.6Hz),3.013-3.032(m,2H,NHCH₂),3.259-3.279(m,2H,NHCH₂),3.751(s,3H,NCH₃),5.365(s,2H,NCH₂),5.749(m,1H,OCH),7.022-7.046(m,2H,ArH),7.195-7.218(m,6H,ArH),7.299-7.328(m,9H,ArH),7.423(m,1H,NH),7.501-7.524(m,1H,NH),7.4674(m,1H,ArH).¹³C NMR(75MHz,CDCl₃):δ171.17,169.04,168.62,155.97,154.09,142.63,142.17,139.57,137.94,136.08,135.64,135.52,134.78,134.58,129.62,129.51,128.95,128.84,127.98,127.39,127.23,127.12,125.89,125.29,123.29,122.11,121.92,118.92,109.09,108.50,73.75,46.50,39.17,39.04,36.28,31.37,29.19,28.79,28.43,27.17,25.87,25.68,21.83,21.33,20.69,16.44,13.60,13.38。

Example 13

4-N- {2- [ (1-acetoxy) N-pentyl]Benzoyl butanediamine telmisartan amide (I)₈) Synthesis of (2)

Referring to the procedure of example 5, the intermediate (1.60g,5.0mmol) and telmisartan (2.57g,5.0mmol) were reacted by column chromatography to give 1.88g of white solid in 46% yield. mp 97-99 deg.C, MS (ESI) M/z 817.5[ M + H]+,839.5[M+Na]+.¹H NMR(300MHz,CDCl₃):δ0.743-0.766(m,3H,CH₃),0.974(t,3H,CH₃,J＝7.3Hz),1.834-1.260(m,12H,6×CH₂),1.835(s,3H,ArCH₃),2.696(s,3H,COCH₃),2.856(t,2H,NCNCH₂,J＝7.7Hz),3.097(m,4H,2×NHCH₂),3.748(s,3H,NCH₃),5.382(s,2H,NCH₂),5.903(m,1H,OCH),6.998-7.024(m,2H,ArH),7.191-7.215(m,6H,ArH),7.280(m,9H,ArH),7.474(m,2H,2×NH),7.638(m,1H,ArH).¹³C NMR(75MHz,CDCl₃):δ170.96,169.22,168.62,156.16,153.91,142.70,139.66,138.30,138.08,135.84,135.30,134.74,129.57,129.33,129.03,128.89,127.87,127.25,127.10,126.84,125.78,125.46,123.27,122.29,121.15,118.69,109.14,108.70,73.52,46.47,38.76,38.52,36.16,31.35,29.16,27.14,26.21,25.69,21.78,21.27,20.52,16.38,13.54,13.31。

Example 14

After 4L fermentation, 1.5g of active fermentation product was obtained, and further compound 1(11mg) and compound 2(8.5mg) were isolated, and MIC values of compound 1 and compound 2 were 12.5 and 3.13. mu.g/mL, respectively.

(1) Large-scale fermentation of strains

And (3) filling 100mL of PDB liquid culture medium into a 250mL conical flask, sterilizing at high temperature of 120 ℃, inoculating a proper amount of bacterial colony, and performing shaking culture at the temperature of 28 ℃ and 120r/min for 3d by a shaking table to obtain a seed solution. 400mL of PDB liquid culture medium is filled in a 1000mL conical flask, high-temperature sterilization is carried out, the seed liquid is added according to the inoculation amount of 1:40, 10 bottles are inoculated, standing culture is carried out for 40d at 28 ℃, and about 4 liters of fermentation liquid is obtained.

(2) Antibacterial Activity test (Filter paper method)

Pouring the sterilized LB solid culture medium into a culture dish, uniformly coating overnight liquid-cultured vibrio parahaemolyticus on the solid culture medium, preparing a sample into a solution with the concentration of 50mg/mL by using methanol, sucking 3 mu L (namely 150 mu g/piece) and dripping the solution onto a filter paper piece with the diameter of 6mm, volatilizing the solvent, attaching a dosing surface to the culture medium, enabling each sample to be parallel, sealing the culture dish with the filter paper piece, placing the culture dish in a constant-temperature incubator at 28 ℃ for inverted culture for 18-24h, and measuring the diameter of a bacteriostatic circle to be 14.5 mm.

(3) Isolation and Structure identification

4L of culture solution is filtered, filtrate is extracted for 2 times by ethyl acetate solution containing 5 percent acetone, the ethyl acetate is collected and concentrated under reduced pressure to obtain 1.5g of total extract. Subjecting the extract to silica gel reduced pressure column chromatography with gradient elution of chloroform/methanol system, wherein Fr.A (160mg) eluted from dichloromethane is subjected to silica gel atmospheric pressure column chromatography with gradient elution of petroleum ether/ethyl acetate, wherein petroleum ether: ethyl acetate 30:1 from elution fr.a-1(50mg) and petroleum ether: fr.a-5(25mg) eluted at 3:1 ethyl acetate was the target component. Fr.a-1 was purified by semi-preparative HPLC with mobile phase methanol: water 90:10 to give compound 1(TA-4,11mg), fr.a-5 purified by semi-preparative HPLC, methanol: 80 percent of water, 20 to obtain the compound 2(TA-7,8.5mg), and the identification result is the same as the above.

(4) Antibacterial Activity test (microdilution method) for Compound 1 and Compound 2

Diluting the prepared bacterial seed culture solution to 1:800 of the original concentration. And (3) taking a 96-hole micro-dilution plate, adding 198 mu L of bacterial liquid into the first row of the plate, then adding 2 mu L of sample, adding 100 mu L of bacterial liquid into the other holes, sucking the uniformly mixed sample and bacterial liquid in the 100 mu L first row, sequentially adding the uniformly mixed sample and bacterial liquid downwards, diluting by twice decreasing concentration, finally filling 200 mu L of each hole, and setting three parallel concentrations. And setting ampicillin sodium positive control, a culture medium blank control group and DMSO negative control, culturing at 28 ℃ for 18-24h, and measuring absorbance at 600nm by using an enzyme-labeling instrument to obtain the minimum inhibitory concentration. In-vitro antibacterial experiments show that the compound 1 and the compound 2 have obvious inhibitory activity on vibrio parahemolyticus, and MIC values are 12.5 mu g/mL and 3.13 mu g/mL respectively. MIC values of the compound 2 to Bacillus subtilis, methicillin-resistant Staphylococcus aureus and enterococcus faecium were 3.13,12.5 and 12.5. mu.g/mL, respectively.

The above description is only of the preferred embodiments of the present invention, and it should be noted that: it will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the principles of the invention and these are intended to be within the scope of the invention.

Claims (5)

1. A marine fungus WH4-2 is characterized by being a pannaga (a)Talaromyces stipitatus) WH4-2, wherein the strain is preserved in China general microbiological culture Collection center in 2018, 1 month and 31 days, the preservation address is No. 3 of West Lu No.1 of Beijing Kogyo-Yang district, and the preservation number of the strain is CGMCC NO. 15280.

2. A process for preparing dibenzoxepin compounds, comprising: the marine fungus WH4-2 as claimed in claim 1 is fermented in large scale by using a fermentation culture medium, the extract is subjected to silica gel reduced pressure column chromatography, and Fr.A eluted by dichloromethane has a remarkable inhibition effect on vibrio parahaemolyticus when the Fr.A is 150 μ g/tablet;

the dibenzoxepin compound is a compound shown as a general formula I; the structural formula of the compound is shown as a formula I;

wherein: r₁Is OH or OCH₃；R₂Is isopentenyl, R₂The substitution position of the isopentenyl group is C-7 position or C-9 position; the C-5 position is S configuration or R configuration.

3. The method of claim 2, wherein: a, silica gel atmospheric pressure column chromatography, eluting with a petroleum ether/ethyl acetate gradient, wherein petroleum ether: ethyl acetate 30:1 from fr.a-1 and petroleum ether: Fr.A-5 eluted from ethyl acetate 3:1 is an anti-vibrio parahaemolyticus active component.

4. The production method according to claim 3, characterized in that: fr.a-1 was purified by semi-preparative HPLC with mobile phase methanol: water 90:10 to give mixture 1;

mixture 1 is a mixture of 5R-Arugosine K and 5S-Arugosine K;

5. the production method according to claim 3, characterized in that: fr.a-5 was purified by semi-preparative HPLC, methanol: obtaining a mixture 2 by 80: 20;

mixture 2 is a mixture of Arugosin N and CAS: 160585-91-1;

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