CN112111399A - Animal cell reaction device capable of controlling temperature and supplying oxygen uniformly - Google Patents
Animal cell reaction device capable of controlling temperature and supplying oxygen uniformly Download PDFInfo
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- CN112111399A CN112111399A CN202011022655.0A CN202011022655A CN112111399A CN 112111399 A CN112111399 A CN 112111399A CN 202011022655 A CN202011022655 A CN 202011022655A CN 112111399 A CN112111399 A CN 112111399A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The invention relates to the technical field of animal cells, and discloses an animal cell reaction device capable of controlling temperature and supplying oxygen uniformly, which comprises a reaction tank, wherein a cover plate is fixedly connected to the inner top of the reaction tank, a stirring shaft is fixedly connected to the bottom of the cover plate, an air pipe is fixedly connected to the inside of the stirring shaft, a connecting rod is movably connected to the top of the water pipe, a transmission wheel is fixedly connected to the front of the connecting rod, a crank wheel is connected to the top of the transmission wheel in a transmission manner, a swing rod is movably connected to the front of the crank wheel, a sliding block is movably connected to the top of the swing rod, and a friction block is fixedly connected to. This can even animal cells reaction unit of controlled temperature oxygen suppliment ventilates through the trachea, and gaseous promotion piston rod removes, and the piston rod drives the valve ball and rotates, and during gaseous admission valve ball, gaseous promotion swing door swing simultaneously, rethread swing door and buffer spring's cooperation was used to the even effect of oxygen suppliment has been reached.
Description
Technical Field
The invention relates to the technical field of animal cells, in particular to an animal cell reaction device capable of controlling temperature and supplying oxygen uniformly.
Background
The animal cell has nucleus, cytoplasm and cell membrane, no cell wall, unobvious vacuole and lysosome, and needs reaction equipment during in vitro culture, which is a key device in the whole culture process and provides a proper growth environment for the cell to rapidly proliferate and form the required biological tissue product.
The existing animal cell reaction device has the advantages that when the cells are cultured, oxygen supply is not uniform enough, the quality of animal cell culture can be influenced, meanwhile, the animal cells need to have a constant-temperature environment when being cultured, heat can be generated during cell culture, heat dissipation is needed, the existing device can only preserve heat, the heat dissipation effect is poor, the local temperature is too high when the cell culture can be caused, and the growth of the cells is influenced.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the animal cell reaction device capable of controlling the temperature and supplying oxygen uniformly, which has the advantages of uniform oxygen supply, capability of ensuring constant culture temperature, good heat dissipation effect, high culture quality and the like, and solves the problems that the quality of animal cell culture is influenced because the oxygen supply is not uniform enough, the existing device only can preserve heat, the heat dissipation effect is poor, and the local temperature is too high during cell culture.
(II) technical scheme
In order to realize the purposes of uniform oxygen supply, constant culture temperature, good heat dissipation effect and high culture quality, the invention provides the following technical scheme: an animal cell reaction device capable of controlling temperature and supplying oxygen uniformly comprises a reaction tank, wherein a cover plate is fixedly connected to the inner top of the reaction tank, a stirring shaft is fixedly connected to the bottom of the cover plate, an air pipe is fixedly connected to the inner part of the stirring shaft, a tension spring is fixedly connected to the inner part of the air pipe, a piston rod is fixedly connected to the top of the tension spring, a valve ball is movably connected to one side of the piston rod away from the tension spring, the inner wall of the valve ball is movably connected with a swing door, a buffer spring is fixedly connected to the bottom of the swing door, a vessel is fixedly connected to the inner part of the reaction tank, a temperature control box is fixedly connected to the outer side of the vessel, a supporting spring is fixedly connected to the inner top of the temperature control box, a push rod is fixedly connected to the bottom of the supporting spring, the top of the water pipe is connected with an ejector rod in a sliding mode, the bottom of the ejector rod is fixedly connected with a return spring, the top of the water pipe is movably connected with a connecting rod, the front of the connecting rod is fixedly connected with a driving wheel, the top of the driving wheel is connected with a crank wheel in a driving mode, the front of the crank wheel is movably connected with a swing rod, the top of the swing rod is movably connected with a sliding block, and the bottom of the utensil is fixedly connected with a friction block.
Preferably, the outer side of the stirring shaft is provided with an air hole which is matched with the valve ball for use, so that oxygen supply is uniform.
Preferably, the valve ball is connected to a gas pipe so that gas can enter the valve ball.
Preferably, the push rod is connected inside the temperature control box in a sliding mode, and transmission stability of the push rod is guaranteed.
Preferably, the water pipe is slidably connected to the inside of the reaction tank, and the water pipe can be stably moved.
Preferably, the driving wheel is in transmission connection with a water wheel, so that the water wheel can drive the driving wheel to rotate.
Preferably, the connecting rod is movably connected in the reaction tank, so that the connecting rod is prevented from interfering the water pipe to rise, and meanwhile, the connecting rod is used for changing the transmission direction.
Preferably, the sliding block is connected to the top of the connecting rod in a sliding manner, so that the sliding block slides left and right.
(III) advantageous effects
Compared with the prior art, the invention provides the animal cell reaction device capable of controlling the temperature and supplying oxygen uniformly, and the animal cell reaction device has the following beneficial effects:
1. this can even animal cells reaction unit of controlled temperature oxygen suppliment ventilates through the trachea, and gaseous promotion piston rod removes, and the piston rod drives the valve ball and rotates, and during gaseous admission valve ball, gaseous promotion swing door swing simultaneously, rethread swing door and buffer spring's cooperation was used to the even effect of oxygen suppliment has been reached.
2. This can even animal cell reaction unit of controlled temperature oxygen suppliment drives the water pipe through the push rod decline and descends, and the water pipe descends and makes the ejector pin rise under reset spring's effect, and the ejector pin rises and makes water wheels rotate, and water wheels drive the drive wheel and rotate, and the drive wheel drives the crank wheel and rotates, and the crank wheel drives the pendulum rod swing, and the pendulum rod drives the slider and slides, and the rethread slider uses with the cooperation of clutch blocks to reached and to guarantee to cultivate constancy of temperature, the good effect of radiating effect.
Drawings
FIG. 1 is a schematic view of the present invention;
FIG. 2 is a partial schematic view of a structural mixer shaft according to the present invention;
FIG. 3 is an enlarged view of part A of FIG. 2;
FIG. 4 is a schematic left side cross-sectional view of a ball valve portion of the present invention construction;
fig. 5 is a partial schematic view of a structural water wheel of the invention.
In the figure: 1. a reaction tank; 2. a cover plate; 3. a stirring shaft; 4. an air tube; 5. a tension spring; 6. a piston rod; 7. a valve ball; 8. a swing door; 9. a buffer spring; 10. a vessel; 11. a temperature control box; 12. a support spring; 13. a push rod; 14. a water pipe; 15. a water wheel; 16. a top rod; 17. a return spring; 18. a driving wheel; 19. a connecting rod; 20. a crank wheel; 21. a swing rod; 22. a slider; 23. a friction block.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, an animal cell reaction device capable of controlling temperature and supplying oxygen uniformly comprises a reaction tank 1, a cover plate 2 fixedly connected to the inner top of the reaction tank 1, a stirring shaft 3 fixedly connected to the bottom of the cover plate 2, an air hole provided on the outer side of the stirring shaft 3 for matching with a valve ball 7 to supply oxygen uniformly, an air pipe 4 fixedly connected to the inner side of the stirring shaft 3, a tension spring 5 fixedly connected to the inner side of the air pipe 4, a piston rod 6 fixedly connected to the top of the tension spring 5, a valve ball 7 movably connected to the side of the piston rod 6 away from the tension spring 5, the valve ball 7 connected to the air pipe 4 to allow gas to enter the valve ball 7, a swing door 8 movably connected to the inner wall of the valve ball 7, a buffer spring 9 fixedly connected to the bottom of the swing door 8, a vessel 10 fixedly connected to the inner side of the reaction tank 1, the liquid in the temperature control box 11 is mercury and is easy to expand when being heated, a support spring 12 is fixedly connected to the inner top of the temperature control box 11, a push rod 13 is fixedly connected to the bottom of the support spring 12, the push rod 13 is slidably connected to the inner portion of the temperature control box 11 to ensure stable transmission of the push rod 13, a water pipe 14 is fixedly connected to the bottom of the push rod 13, water in the water pipe 14 flows all the time, the water pipe 14 is slidably connected to the inner portion of the reaction tank 1, the water pipe 14 can move stably, a water wheel 15 is fixedly connected to the inner portion of the water pipe 14, a push rod 16 is slidably connected to the top of the water pipe 14, a return spring 17 is fixedly connected to the bottom of the push rod 16, a connecting rod 19 is movably connected to the top of the water pipe 14, the connecting rod 19 is movably connected to the inner portion of the reaction tank 1 to ensure that the, the water wheel 15 can drive the transmission wheel 18 to rotate, the top of the transmission wheel 18 is in transmission connection with the crank wheel 20, the front face of the crank wheel 20 is movably connected with the swing rod 21, the top of the swing rod 21 is movably connected with the sliding block 22, the sliding block 22 is in sliding connection with the top of the connecting rod 19, the sliding block 22 slides left and right, the bottom of the utensil 10 is fixedly connected with the friction block 23, and the friction block 23 generates heat through friction, so that energy can be saved.
The working principle is that when reaction is needed, the air cylinder drives the cover plate 2 to descend, the cover plate 2 drives the stirring shaft 3 to descend, the stirring shaft 3 descends into the vessel 10, the air pipe 4 is ventilated, the air pushes the piston rod 6 to move towards the valve ball 7, the piston rod 6 drives the valve ball 7 to rotate, the valve ball 7 drives the swing door 8 to rotate to the air hole position outside the stirring shaft 3, meanwhile, the air enters the valve ball 7 through the air pipe 4, the air pushes the swing door 8 to swing towards the outside of the valve ball 7, the valve ball 7 is exhausted, the swing door 8 swings towards the outside to be conical, cells are prevented from entering the valve ball 7, meanwhile, when the stirring shaft 3 is stirred, the oxygen can be uniformly supplied into the cells, when the stirring is completed, the air pipe 4 stops ventilating, the piston rod 6 is far away from the valve ball 7 under the action of the tension spring 5, the piston rod 6 drives the valve ball 7 to rotate, the valve ball, when the temperature in the vessel 10 is raised by cell reaction, mercury in the temperature control box 11 is heated to expand, the mercury expands to drive the push rod 13 to rise, the push rod 13 rises to drive the water pipe 14 to rise, the water pipe 14 rises to drive the connecting rod 19 to swing, the connecting rod 19 swings to enable the sliding block 22 to be far away from the friction block 23, when the water pipe 14 rises to be in contact with the vessel 10, the vessel 10 pushes the push rod 16 to fall, the push rod 16 falls to clamp the water wheel 15, at the moment, water flow in the water pipe 14 enables the vessel 10 to cool, so that the vessel 10 can effectively dissipate heat, when the temperature in the vessel 10 is lower, water silver in the temperature control box 11 contracts, the push rod 13 falls to drive the water pipe 14 to fall, the water pipe 14 falls to drive the connecting rod 19 to swing, the connecting rod 19 swings to drive the sliding block 22 to be in contact with the, the water wheel 15 rotates to drive the transmission wheel 18 to rotate, the transmission wheel 18 rotates to drive the crank wheel 20 to rotate, the crank wheel 20 rotates to drive the swing rod 21 to swing, the swing rod 21 swings to drive the slide block 22 to slide, the slide block 22 slides to rub the friction block 23, and the friction block 23 generates heat, so that the temperature of the vessel 10 is raised, and the reaction temperature is constant.
To sum up, this can even animal cells reaction unit of controlled temperature oxygen suppliment ventilates through trachea 4, and gaseous promotion piston rod 6 removes, and piston rod 6 drives valve ball 7 and rotates, and gaseous entering valve ball 7 simultaneously, and gaseous promotion swing door 8 swings, uses through the cooperation of swing door 8 with buffer spring 9 again to the even effect of oxygen suppliment has been reached. This can control even animal cells reaction unit of temperature oxygen suppliment, it descends to drive water pipe 14 through push rod 13 descends, water pipe 14 descends and makes ejector pin 16 rise under reset spring 17's effect, ejector pin 16 rises and makes water wheels 15 rotate, water wheels 15 drive wheel 18 and rotates, drive wheel 18 drives crank wheel 20 and rotates, crank wheel 20 drives the pendulum rod 21 swing, pendulum rod 21 drives slider 22 and slides, and the cooperation of rethread slider 22 and clutch blocks 23 is used, thereby reached and to guarantee to cultivate the constancy of temperature, the good effect of radiating effect, application scope is more extensive.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides an animal cell reaction unit that can control temperature oxygen suppliment is even, includes retort (1), its characterized in that: the reaction tank is characterized in that the cover plate (2) is fixedly connected to the inner top of the reaction tank (1), the stirring shaft (3) is fixedly connected to the bottom of the cover plate (2), the air pipe (4) is fixedly connected to the inner portion of the stirring shaft (3), the tension spring (5) is fixedly connected to the inner portion of the air pipe (4), the piston rod (6) is fixedly connected to the top of the tension spring (5), the valve ball (7) is movably connected to one side, away from the tension spring (5), of the piston rod (6), the swing door (8) is movably connected to the inner wall of the valve ball (7), the buffer spring (9) is fixedly connected to the bottom of the swing door (8), the vessel (10) is fixedly connected to the inner portion of the reaction tank (1), the temperature control box (11) is fixedly connected to the outer side of the vessel (10), and the supporting, the bottom fixedly connected with push rod (13) of supporting spring (12), the bottom fixedly connected with water pipe (14) of push rod (13), the inside fixedly connected with water wheels (15) of water pipe (14), the top sliding connection of water pipe (14) has ejector pin (16), the bottom fixedly connected with reset spring (17) of ejector pin (16), the top swing joint of water pipe (14) has connecting rod (19), the positive fixedly connected with drive wheel (18) of connecting rod (19), the top transmission of drive wheel (18) is connected with crank wheel (20), the positive swing joint of crank wheel (20) has pendulum rod (21), the top swing joint of pendulum rod (21) has slider (22), the bottom fixedly connected with clutch blocks (23) of household utensils (10).
2. The apparatus for reacting animal cells capable of controlling temperature and supplying oxygen uniformly as claimed in claim 1, wherein: the outer side of the stirring shaft (3) is provided with air holes.
3. The apparatus for reacting animal cells capable of controlling temperature and supplying oxygen uniformly as claimed in claim 1, wherein: the valve ball (7) is connected with the air pipe (4).
4. The apparatus for reacting animal cells capable of controlling temperature and supplying oxygen uniformly as claimed in claim 1, wherein: the push rod (13) is connected inside the temperature control box (11) in a sliding mode.
5. The apparatus for reacting animal cells capable of controlling temperature and supplying oxygen uniformly as claimed in claim 1, wherein: the water pipe (14) is connected to the inside of the reaction tank (1) in a sliding manner.
6. The apparatus for reacting animal cells capable of controlling temperature and supplying oxygen uniformly as claimed in claim 1, wherein: the transmission wheel (18) is in transmission connection with the water wheel (15).
7. The apparatus for reacting animal cells capable of controlling temperature and supplying oxygen uniformly as claimed in claim 1, wherein: the connecting rod (19) is movably connected inside the reaction tank (1).
8. The apparatus for reacting animal cells capable of controlling temperature and supplying oxygen uniformly as claimed in claim 1, wherein: the sliding block (22) is connected to the top of the connecting rod (19) in a sliding mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011022655.0A CN112111399A (en) | 2020-09-25 | 2020-09-25 | Animal cell reaction device capable of controlling temperature and supplying oxygen uniformly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011022655.0A CN112111399A (en) | 2020-09-25 | 2020-09-25 | Animal cell reaction device capable of controlling temperature and supplying oxygen uniformly |
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| Publication Number | Publication Date |
|---|---|
| CN112111399A true CN112111399A (en) | 2020-12-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011022655.0A Withdrawn CN112111399A (en) | 2020-09-25 | 2020-09-25 | Animal cell reaction device capable of controlling temperature and supplying oxygen uniformly |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112834396A (en) * | 2020-12-30 | 2021-05-25 | 刘艳娜 | Can detect not co-altitude moisture interference's empty gas detection surveys device |
| CN113717844A (en) * | 2021-09-07 | 2021-11-30 | 苏州硕亚环保科技有限公司 | Riverway indigenous PGPR microorganism growth promoting equipment |
-
2020
- 2020-09-25 CN CN202011022655.0A patent/CN112111399A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112834396A (en) * | 2020-12-30 | 2021-05-25 | 刘艳娜 | Can detect not co-altitude moisture interference's empty gas detection surveys device |
| CN112834396B (en) * | 2020-12-30 | 2024-04-30 | 西华县利顺信息技术有限公司 | Air detection device capable of detecting waterproof vapor interference at different heights |
| CN113717844A (en) * | 2021-09-07 | 2021-11-30 | 苏州硕亚环保科技有限公司 | Riverway indigenous PGPR microorganism growth promoting equipment |
| CN113717844B (en) * | 2021-09-07 | 2023-12-12 | 苏州硕亚环保科技有限公司 | River course local PGPR microorganism device of hastening parturition |
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Application publication date: 20201222 |