CN112105730B - 热不稳定蛋白酶 - Google Patents

热不稳定蛋白酶 Download PDF

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Publication number
CN112105730B
CN112105730B CN201980017089.3A CN201980017089A CN112105730B CN 112105730 B CN112105730 B CN 112105730B CN 201980017089 A CN201980017089 A CN 201980017089A CN 112105730 B CN112105730 B CN 112105730B
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sample
protease
concentration
minutes
seq
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Chinese (zh)
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CN112105730A (zh
Inventor
B·K·斯特里伯尼
C·佩德森
J·R·亨利克森
O·拉内斯
M·S·洛伦森
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Arctic Enzyme AS
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Arctic Enzyme AS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201980017089.3A 2018-03-07 2019-03-07 热不稳定蛋白酶 Active CN112105730B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1803654.1 2018-03-07
GBGB1803654.1A GB201803654D0 (en) 2018-03-07 2018-03-07 Thermolabile proteinases
PCT/EP2019/055724 WO2019170809A1 (en) 2018-03-07 2019-03-07 Thermolabile proteinases

Publications (2)

Publication Number Publication Date
CN112105730A CN112105730A (zh) 2020-12-18
CN112105730B true CN112105730B (zh) 2024-10-18

Family

ID=61972698

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980017089.3A Active CN112105730B (zh) 2018-03-07 2019-03-07 热不稳定蛋白酶

Country Status (10)

Country Link
US (1) US11591584B2 (https=)
EP (1) EP3762490A1 (https=)
JP (3) JP2021516993A (https=)
KR (1) KR20200142001A (https=)
CN (1) CN112105730B (https=)
BR (1) BR112020018050A2 (https=)
CA (1) CA3092916A1 (https=)
GB (1) GB201803654D0 (https=)
WO (1) WO2019170809A1 (https=)
ZA (1) ZA202006186B (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL244825B1 (pl) 2021-11-26 2024-03-11 Blirt Spolka Akcyjna Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017006266A1 (en) * 2015-07-06 2017-01-12 Denovo Genomics, Inc. Variants of the subtilisin carlsberg polypeptide with decreased thermostability

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3012377B2 (ja) * 1991-10-11 2000-02-21 名糖産業株式会社 低耐熱性プロテアーゼとその関連物及びその生産法
JPH06261752A (ja) * 1993-03-17 1994-09-20 Amano Pharmaceut Co Ltd 新規セリンプロテイナーゼ及びその製造法
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
JP3664804B2 (ja) * 1996-05-10 2005-06-29 花王株式会社 低温アルカリプロテアーゼｘ、それを生産する微生物、その製造法、並びに当該酵素を含有する洗浄剤組成物及び食品加工用酵素製剤
US5981235A (en) * 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
EP1476573B1 (en) 2002-01-28 2010-01-06 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
EP1769240B1 (en) * 2004-06-17 2011-11-09 ZyGEM Corporation Limited Purification methods and uses thereof
DE102005028295A1 (de) 2005-06-18 2006-11-16 Henkel Kgaa Proteasen aus psychrophilen Organismen
EP2147119B1 (en) 2007-05-03 2011-05-25 Roche Diagnostics GmbH SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
JP5283154B2 (ja) * 2008-01-22 2013-09-04 長瀬産業株式会社 トリプシン様酵素
ES2539856T3 (es) 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
CN101586086B (zh) * 2009-05-15 2011-05-04 南京工业大学 一种耐有机溶剂蛋白酶及其产生菌株
JP2016036327A (ja) * 2014-08-11 2016-03-22 国立大学法人福島大学 プロテアーゼ含有洗浄組成物及びプロテアーゼの安定化方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017006266A1 (en) * 2015-07-06 2017-01-12 Denovo Genomics, Inc. Variants of the subtilisin carlsberg polypeptide with decreased thermostability

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Atle Noralf Larsen 等.Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia.The FEBS Journal.2006,第273卷(第1期),图1、图7以及第52页左栏第2段. *
Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia;Atle Noralf Larsen 等;The FEBS Journal;第273卷(第1期);图1、图7以及第52页左栏第2段 *

Also Published As

Publication number Publication date
JP2024178230A (ja) 2024-12-24
WO2019170809A1 (en) 2019-09-12
CN112105730A (zh) 2020-12-18
GB201803654D0 (en) 2018-04-25
CA3092916A1 (en) 2019-09-12
JP2021516993A (ja) 2021-07-15
JP2024160310A (ja) 2024-11-13
EP3762490A1 (en) 2021-01-13
BR112020018050A2 (pt) 2020-12-29
JP7854473B2 (ja) 2026-05-01
US20200407701A1 (en) 2020-12-31
ZA202006186B (en) 2021-10-27
US11591584B2 (en) 2023-02-28
KR20200142001A (ko) 2020-12-21

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